Restriction map for
pFN21ASDA1349
(Circular) (Six-base) MAPSORT of: pFN21ASDA1349.seq Check: 4087 from: 1 to: 6946
pFN21ASDA1349 6946 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4756 278
Size: 53 83 186 936 3220 2468
Fragments arranged by size:
3220 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4289 1937
Size: 2352 4594
Acc65I G'GTAC_C
Cuts at: 4267 4267
Size: 6946
AclI AA'CG_TT
Cuts at: 1563 5073 5446 1563
Size: 3510 373 3063
Fragments arranged by size:
3510 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2094 2409 2982 3402 3910 4078 4162 5009
Size: 462 315 573 420 508 168 84 847
Cuts at: 5009 6393 6661 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 847 573 508 462 420 315
268 168 84
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6749
AflIII A'CryG_T
Cuts at: 1203 3856 5845 6822 1203
Size: 2653 1989 977 1327
Fragments arranged by size:
2653 1989 1327 977
AgeI A'CCGG_T
Cuts at: 5777 5777
Size: 6946
AhdI GACnn_n'nnGTC
Cuts at: 5675 5675
Size: 6946
AleI CACnn'nnGTG
Cuts at: 1166 2877 1166
Size: 1711 5235
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6946
AlwNI CAG_nnn'CTG
Cuts at: 4689 6261 4689
Size: 1572 5374
ApaLI G'TGCA_C
Cuts at: 3698 5003 6159 3698
Size: 1305 1156 4485
Fragments arranged by size:
4485 1305 1156
ApoI r'AATT_y
Cuts at: 1493 2332 2344 2350 2700 2784 3184 3204 3456
Size: 839 12 6 350 84 400 20 252
Cuts at: 3456 3540 4044 4255 4481 4633 6742 1493
Size: 84 504 211 226 152 2109 1697
Fragments arranged by size:
2109 1697 839 504 400 350 252 226
211 152 84 84 20 12 6
AseI AT'TA_AT
Cuts at: 160 1032 3258 5500 160
Size: 872 2226 2242 1606
Fragments arranged by size:
2242 2226 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6946
AvaI C'yCGr_G
Cuts at: 1870 1943 4271 1870
Size: 73 2328 4545
Fragments arranged by size:
4545 2328 73
BamHI G'GATC_C
Cuts at: 4276 4643 4276
Size: 367 6579
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4267 5722 618
Size: 325 217 627 2480 1455 1842
Fragments arranged by size:
2480 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2031 4265 1391
Size: 640 2234 4072
Fragments arranged by size:
4072 2234 640
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2234 2957 928
Size: 971 335 723 4917
Fragments arranged by size:
4917 971 723 335
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5137 5171 1903
Size: 34 3200 34 3678
Fragments arranged by size:
3678 3200 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4838 6048 4838
Size: 1210 5736
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6946
BfrBI ATG'CAT
Cuts at: 3292 4132 3292
Size: 840 6106
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5557 136
Size: 107 122 71 631 605 3885 1525
Fragments arranged by size:
3885 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6941 6941
Size: 6946
BlpI GC'TnA_GC
Cuts at: 4363 4363
Size: 6946
Bme1580I G_kGCm'C
Cuts at: 1790 2764 3702 5007 6163 1790
Size: 974 938 1305 1156 2573
Fragments arranged by size:
2573 1305 1156 974 938
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6946
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3138 4062 4677
Size: 416 53 83 399 24 1721 924 615
Cuts at: 4677 5635 442
Size: 958 1753
Fragments arranged by size:
1753 1721 958 924 615 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6946
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2646 2814 3066 3486 3570 3654 3738 3822
Size: 1407 168 252 420 84 84 84 84
Cuts at: 3822 3990 4158 5606 1239
Size: 168 168 1448 2579
Fragments arranged by size:
2579 1448 1407 420 252 168 168 168
84 84 84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2207 3509 4144 5071 5936 6234 2207
Size: 1302 635 927 865 298 2919
Fragments arranged by size:
2919 1302 927 865 635 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5609 882
Size: 617 4110 2219
Fragments arranged by size:
4110 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4243 4737 493
Size: 711 3039 494 2702
Fragments arranged by size:
3039 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 2260 6940 2260
Size: 4680 2266
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4753 5135 5770 275
Size: 53 83 186 936 3220 382 635 1451
Fragments arranged by size:
3220 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5379 5777 6051 6198 6609 5379
Size: 398 274 147 411 5716
Fragments arranged by size:
5716 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6721
Fragments arranged by size:
6721 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6946
BseYI C'CCAG_C
Cuts at: 6149 6645 6149
Size: 496 6450
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6373
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4396 5157 6185 1540
Size: 275 186 2395 761 1028 2301
Fragments arranged by size:
2395 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3702 5007 5092 6163 3702
Size: 1305 85 1071 4485
Fragments arranged by size:
4485 1305 1071 85
BsmI GAATG_Cn'
Cuts at: 2053 2825 3167 3251 3294 3503 4457 4550 2053
Size: 772 342 84 43 209 954 93 4449
Fragments arranged by size:
4449 954 772 342 209 93 84 43
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2031 2764 3702 4265 5007 5092 6163
Size: 399 241 733 938 563 742 85 1071
Cuts at: 6163 1391
Size: 2174
Fragments arranged by size:
2174 1071 938 742 733 563 399 241
85
BspEI T'CCGG_A
Cuts at: 6609 6609
Size: 6946
BspHI T'CATG_A
Cuts at: 4835 6565 4835
Size: 1730 5216
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2044 4301 844
Size: 1200 2257 3489
Fragments arranged by size:
3489 2257 1200
BsrBI CCG'CTC
Cuts at: 1394 1823 4833 1394
Size: 429 3010 3507
Fragments arranged by size:
3507 3010 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2286 2510 2667 3409 3745 5441 5615
Size: 1183 1038 224 157 742 336 1696 174
Cuts at: 5615 65
Size: 1396
Fragments arranged by size:
1696 1396 1183 1038 742 336 224 174
157
BsrFI r'CCGG_y
Cuts at: 1700 1810 5590 5777 1700
Size: 110 3780 187 2869
Fragments arranged by size:
3780 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2941 3277 3445 3613 4117 4201 96
Size: 2845 336 168 168 504 84 2841
Fragments arranged by size:
2845 2841 504 336 168 168 84
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6946
BssSI C'ACGA_G
Cuts at: 5006 6018 5006
Size: 1012 5934
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6946
BstBI TT'CG_AA
Cuts at: 2007 5750 2007
Size: 3743 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6946
BstYI r'GATC_y
Cuts at: 1972 4276 4643 5028 5045 6497 6583 6595 6941
Size: 2304 367 385 17 1452 86 12 346
Cuts at: 6941 1972
Size: 1977
Fragments arranged by size:
2304 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5437
Fragments arranged by size:
5437 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3163 487
Size: 683 56 1937 4270
Fragments arranged by size:
4270 1937 683 56
BtsI GCAGTG_nn'
Cuts at: 4462 5256 5276 4462
Size: 794 20 6132
Fragments arranged by size:
6132 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6946
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2971 3006 3391 3426 3727 3762 529
Size: 35 2407 35 385 35 301 35 3713
Fragments arranged by size:
3713 2407 385 301 35 35 35 35
DraI TTT'AAA
Cuts at: 2349 3817 4250 2349
Size: 1468 433 5045
Fragments arranged by size:
5045 1468 433
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5953 809
Size: 507 4637 1802
Fragments arranged by size:
4637 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4393 5282 6911
Size: 54 1425 50 275 18 2563 889 1629
Cuts at: 6911 8
Size: 43
Fragments arranged by size:
2563 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4393 1537
Size: 275 2581 4090
Fragments arranged by size:
4090 2581 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2292 2958 3378 4876 1353
Size: 939 666 420 1498 3423
Fragments arranged by size:
3423 1498 939 666 420
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5533 5907 6053 387
Size: 1064 138 3944 374 146 1280
Fragments arranged by size:
3944 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2094 2409 2646 2814 2982 3066 3402
Size: 393 462 315 237 168 168 84 336
Cuts at: 3402 3486 3570 3654 3738 3822 3910 3990 4078
Size: 84 84 84 84 84 88 80 88
Cuts at: 4078 4158 4162 5009 5606 6393 6661 1239
Size: 80 4 847 597 787 268 1524
Fragments arranged by size:
1524 847 787 597 462 393 336 315
268 237 168 168 88 88 84 84
84 84 84 84 80 80 4
EcoNI CCTnn'n_nnAGG
Cuts at: 3928 3928
Size: 6946
EcoO109I rG'GnC_Cy
Cuts at: 3624 4062 3624
Size: 438 6508
EcoRI G'AATT_C
Cuts at: 2700 2784 3204 3456 4044 4255 4633 2700
Size: 84 420 252 588 211 378 5013
Fragments arranged by size:
5013 588 420 378 252 211 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2439 2439
Size: 6946
FspI TGC'GCA
Cuts at: 5452 5452
Size: 6946
HaeII r_GCGC'y
Cuts at: 1678 6093 1678
Size: 4415 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2499 2531 5587 5619 5661
Size: 32 135 32 927 32 3056 32 42
Cuts at: 5661 5693 1373
Size: 32 2626
Fragments arranged by size:
3056 2626 927 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4290 4536 669
Size: 988 281 2352 246 3079
Fragments arranged by size:
3079 2352 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2829 748
Size: 2081 4865
HpaI GTT'AAC
Cuts at: 4536 4536
Size: 6946
KpnI G_GTAC'C
Cuts at: 4271 4271
Size: 6946
MfeI C'AATT_G
Cuts at: 4545 4545
Size: 6946
MluI A'CGCG_T
Cuts at: 6822 6822
Size: 6946
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6060 6244 1305
Size: 4755 184 2007
Fragments arranged by size:
4755 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5124
Fragments arranged by size:
5124 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2877 2977 3481 3512 3649 3817 4904
Size: 648 1711 100 504 31 137 168 1087
Cuts at: 4904 5263 5422 518
Size: 359 159 2042
Fragments arranged by size:
2042 1711 1087 648 504 359 168 159
137 100 31
MspA1I CmG'CkG
Cuts at: 1606 4362 5039 6187 6432 1606
Size: 2756 677 1148 245 2120
Fragments arranged by size:
2756 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5437
Fragments arranged by size:
5437 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6946
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6946
NotI GC'GGCC_GC
Cuts at: 4393 4393
Size: 6946
NsiI A_TGCA'T
Cuts at: 3294 4134 3294
Size: 840 6106
NspI r_CATG'y
Cuts at: 1132 1229 2487 3860 4304 5849 1132
Size: 97 1258 1373 444 1545 2229
Fragments arranged by size:
2229 1545 1373 1258 444 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6946
PciI A'CATG_T
Cuts at: 3856 5845 3856
Size: 1989 4957
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6946
PmeI GTTT'AAAC
Cuts at: 4250 4250
Size: 6946
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5015 5047 6528 6560 5015
Size: 32 1481 32 5401
Fragments arranged by size:
5401 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3624 3624
Size: 6946
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6946
PsiI TTA'TAA
Cuts at: 3312 4516 3312
Size: 1204 5742
PstI C_TGCA'G
Cuts at: 830 4298 830
Size: 3468 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6946
SalI G'TCGA_C
Cuts at: 1936 4288 1936
Size: 2352 4594
SapI GCTCTTCn'nnn_
Cuts at: 2292 2292
Size: 6946
SbfI CC_TGCA'GG
Cuts at: 4298 4298
Size: 6946
ScaI AGT'ACT
Cuts at: 5194 6906 5194
Size: 1712 5234
SfcI C'TryA_G
Cuts at: 826 1045 3085 4294 5429 6110 6301 826
Size: 219 2040 1209 1135 681 191 1471
Fragments arranged by size:
2040 1471 1209 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6946
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6946
SmaI CCC'GGG
Cuts at: 1872 4273 1872
Size: 2401 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2222 3524 4123 5050 5951 6213
Size: 197 926 279 1302 599 927 901 262
Cuts at: 6213 820
Size: 1553
Fragments arranged by size:
1553 1302 927 926 901 599 279 262
197
SnaBI TAC'GTA
Cuts at: 493 4243 4737 493
Size: 3750 494 2702
Fragments arranged by size:
3750 2702 494
SpeI A'CTAG_T
Cuts at: 152 2237 152
Size: 2085 4861
SphI G_CATG'C
Cuts at: 1132 4304 1132
Size: 3172 3774
SspI AAT'ATT
Cuts at: 5 52 4870 5
Size: 47 4818 2081
Fragments arranged by size:
4818 2081 47
StuI AGG'CCT
Cuts at: 1517 2495 2663 3335 1517
Size: 978 168 672 5128
Fragments arranged by size:
5128 978 672 168
StyI C'CwwG_G
Cuts at: 513 1065 2022 4385 513
Size: 552 957 2363 3074
Fragments arranged by size:
3074 2363 957 552
SwaI ATTT'AAAT
Cuts at: 2349 3817 2349
Size: 1468 5478
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4973 4990 5143 5328 5773 325
Size: 1255 3393 17 153 185 445 1498
Fragments arranged by size:
3393 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2480 2941 3277
Size: 274 80 33 51 1443 503 461 336
Cuts at: 3277 3317 3445 3485 3613 4117 4201 5192 6904
Size: 40 128 40 128 504 84 991 1712
Cuts at: 6904 96
Size: 138
Fragments arranged by size:
1712 1443 991 504 503 461 336 274
138 128 128 84 80 51 40 40
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6906 6938 6906
Size: 32 6914
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6946
XbaI T'CTAG_A
Cuts at: 4282 4282
Size: 6946
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6946
XmaI C'CCGG_G
Cuts at: 1870 4271 1870
Size: 2401 4545
XmnI GAAnn'nnTTC
Cuts at: 2265 2301 2579 3094 3167 3251 3671 5075 2265
Size: 36 278 515 73 84 420 1404 4136
Fragments arranged by size:
4136 1404 515 420 278 84 73 36
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4754 276
Size: 53 83 186 936 3220 2468
Fragments arranged by size:
3220 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI XcmI