Restriction map for
pFN21ASDA1345
(Circular) (Six-base) MAPSORT of: pFN21ASDA1345.seq Check: 5551 from: 1 to: 9265
pFN21ASDA1345 9265 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2575 2575
Size: 9265
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7075 278
Size: 53 83 186 936 5539 2468
Fragments arranged by size:
5539 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5646 6489 6608 1937
Size: 3709 843 119 4594
Fragments arranged by size:
4594 3709 843 119
Acc65I G'GTAC_C
Cuts at: 6586 6586
Size: 9265
AclI AA'CG_TT
Cuts at: 1563 7392 7765 1563
Size: 5829 373 3063
Fragments arranged by size:
5829 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4037 4173 5247 6159 6426 7328 8712 8980
Size: 2405 136 1074 912 267 902 1384 268
Cuts at: 8980 1632
Size: 1917
Fragments arranged by size:
2405 1917 1384 1074 912 902 268 267
136
AfeI AGC'GCT
Cuts at: 3914 6158 3914
Size: 2244 7021
AflII C'TTAA_G
Cuts at: 820 1017 3392 820
Size: 197 2375 6693
Fragments arranged by size:
6693 2375 197
AflIII A'CryG_T
Cuts at: 1203 5600 8164 9141 1203
Size: 4397 2564 977 1327
Fragments arranged by size:
4397 2564 1327 977
AgeI A'CCGG_T
Cuts at: 8096 8096
Size: 9265
AhdI GACnn_n'nnGTC
Cuts at: 3966 7994 3966
Size: 4028 5237
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9265
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9265
AlwNI CAG_nnn'CTG
Cuts at: 7008 8580 7008
Size: 1572 7693
ApaLI G'TGCA_C
Cuts at: 7322 8478 7322
Size: 1156 8109
ApoI r'AATT_y
Cuts at: 1493 2165 2300 3082 3218 3336 3353 3528 4643
Size: 672 135 782 136 118 17 175 1115
Cuts at: 4643 5060 6055 6473 6574 6800 6952 9061 1493
Size: 417 995 418 101 226 152 2109 1697
Fragments arranged by size:
2109 1697 1115 995 782 672 418 417
226 175 152 136 135 118 101 17
AseI AT'TA_AT
Cuts at: 160 1032 7819 160
Size: 872 6787 1606
Fragments arranged by size:
6787 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9265
AvaI C'yCGr_G
Cuts at: 1870 1943 3710 3889 6590 1870
Size: 73 1767 179 2701 4545
Fragments arranged by size:
4545 2701 1767 179 73
BamHI G'GATC_C
Cuts at: 2922 6595 6962 2922
Size: 3673 367 5225
Fragments arranged by size:
5225 3673 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4636 6586 8041 618
Size: 325 217 627 2849 1950 1455 1842
Fragments arranged by size:
2849 1950 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2079 3747 3819 4147 5278 5637 6584 1391
Size: 688 1668 72 328 1131 359 947 4072
Fragments arranged by size:
4072 1668 1131 947 688 359 328 72
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 5073 928
Size: 971 3174 5120
Fragments arranged by size:
5120 3174 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5190 5224 7456 7490 1903
Size: 34 3253 34 2232 34 3678
Fragments arranged by size:
3678 3253 2232 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3717 4308 7157 8367 3717
Size: 591 2849 1210 4615
Fragments arranged by size:
4615 2849 1210 591
BclI T'GATC_A
Cuts at: 1548 3057 5761 1548
Size: 1509 2704 5052
Fragments arranged by size:
5052 2704 1509
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3167 4436 7876
Size: 107 122 71 631 605 1495 1269 3440
Cuts at: 7876 136
Size: 1525
Fragments arranged by size:
3440 1525 1495 1269 631 605 122 107
71
BglII A'GATC_T
Cuts at: 3062 9260 3062
Size: 6198 3067
BlpI GC'TnA_GC
Cuts at: 3836 6180 6682 3836
Size: 2344 502 6419
Fragments arranged by size:
6419 2344 502
Bme1580I G_kGCm'C
Cuts at: 1790 3875 4221 7326 8482 1790
Size: 2085 346 3105 1156 2573
Fragments arranged by size:
3105 2573 2085 1156 346
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9265
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2271 2736 3166
Size: 416 53 83 399 24 854 465 430
Cuts at: 3166 6996 7954 442
Size: 3830 958 1753
Fragments arranged by size:
3830 1753 958 854 465 430 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 4097 1056
Size: 3041 6224
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2145 2747 2145
Size: 602 8663
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3087 3646 3723 4565 4775 5770 6250 6462
Size: 1848 559 77 842 210 995 480 212
Cuts at: 6462 7925 1239
Size: 1463 2579
Fragments arranged by size:
2579 1848 1463 995 842 559 480 212
210 77
Bpu10I CC'TnA_GC
Cuts at: 4901 4901
Size: 9265
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2350 7390 8255 8553 2350
Size: 5040 865 298 3062
Fragments arranged by size:
5040 3062 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3063 3892 7928 882
Size: 617 1564 829 4036 2219
Fragments arranged by size:
4036 2219 1564 829 617
BsaAI yAC'GTr
Cuts at: 493 1204 2653 5124 6562 7056 493
Size: 711 1449 2471 1438 494 2702
Fragments arranged by size:
2702 2471 1449 1438 711 494
BsaBI GATnn'nnATC
Cuts at: 6037 9259 6037
Size: 3222 6043
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 7072 7454 8089 275
Size: 53 83 186 936 5539 382 635 1451
Fragments arranged by size:
5539 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2106 7698 8096 8370 8517 8928 2106
Size: 5592 398 274 147 411 2443
Fragments arranged by size:
5592 2443 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3442 3472 4939 4969 6306
Size: 30 165 30 2039 30 1467 30 1337
Cuts at: 6306 6336 1178
Size: 30 4107
Fragments arranged by size:
4107 2039 1467 1337 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2087 2241 2552 2555 2592 3584 3728 3817
Size: 901 154 311 3 37 992 144 89
Cuts at: 3817 5616 1186
Size: 1799 4835
Fragments arranged by size:
4835 1799 992 901 311 154 144 89
37 3
BseYI C'CCAG_C
Cuts at: 3731 5422 6358 8468 8964 3731
Size: 1691 936 2110 496 4032
Fragments arranged by size:
4032 2110 1691 936 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2087 2373 2553 1163
Size: 573 351 286 180 7875
Fragments arranged by size:
7875 573 351 286 180
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6715 7476 8504 1540
Size: 275 186 4714 761 1028 2301
Fragments arranged by size:
4714 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3564 5637 5756 7326 7411 8482 3564
Size: 2073 119 1570 85 1071 4347
Fragments arranged by size:
4347 2073 1570 1071 119 85
BsmI GAATG_Cn'
Cuts at: 6776 6869 6776
Size: 93 9172
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2079 3564 3747 3819 3875 4147 4221
Size: 399 289 1485 183 72 56 272 74
Cuts at: 4221 5278 5637 5756 6584 7326 7411 8482 1391
Size: 1057 359 119 828 742 85 1071 2174
Fragments arranged by size:
2174 1485 1071 1057 828 742 399 359
289 272 183 119 85 74 72 56
BspEI T'CCGG_A
Cuts at: 2106 8928 2106
Size: 6822 2443
BspHI T'CATG_A
Cuts at: 7154 8884 7154
Size: 1730 7535
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2575 2689 4786 6620 844
Size: 1731 114 2097 1834 3489
Fragments arranged by size:
3489 2097 1834 1731 114
BsrBI CCG'CTC
Cuts at: 1394 1823 2522 3881 7152 1394
Size: 429 699 1359 3271 3507
Fragments arranged by size:
3507 3271 1359 699 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2228 3386 5869 7760 7934 65
Size: 1183 980 1158 2483 1891 174 1396
Fragments arranged by size:
2483 1891 1396 1183 1158 980 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5047 7909 8096 1700
Size: 110 3237 2862 187 2869
Fragments arranged by size:
3237 2869 2862 187 110
BsrGI T'GTAC_A
Cuts at: 96 2785 96
Size: 2689 6576
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9265
BssSI C'ACGA_G
Cuts at: 4196 7325 8337 4196
Size: 3129 1012 5124
Fragments arranged by size:
5124 3129 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2225 794
Size: 1431 7834
BstBI TT'CG_AA
Cuts at: 2007 8069 2007
Size: 6062 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9265
BstXI CCAn_nnnn'nTGG
Cuts at: 2800 2800
Size: 9265
BstYI r'GATC_y
Cuts at: 1972 2922 3062 4595 6528 6595 6962 7347 7364
Size: 950 140 1533 1933 67 367 385 17
Cuts at: 7364 8816 8902 8914 9260 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1933 1533 1452 950 385 367 346
140 86 67 17 12
BstZ17I GTA'TAC
Cuts at: 6490 6490
Size: 9265
Bsu36I CC'TnA_GG
Cuts at: 4267 5628 5874 4267
Size: 1361 246 7658
Fragments arranged by size:
7658 1361 246
BtgI C'CryG_G
Cuts at: 513 1065 2022 2117 513
Size: 552 957 95 7661
Fragments arranged by size:
7661 957 552 95
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8526
Fragments arranged by size:
8526 683 56
BtsI GCAGTG_nn'
Cuts at: 3765 4934 5058 5944 6781 7575 7595 3765
Size: 1169 124 886 837 794 20 5435
Fragments arranged by size:
5435 1169 886 837 794 124 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9265
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9230
DraI TTT'AAA
Cuts at: 5619 6569 5619
Size: 950 8315
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4080 8272 809
Size: 507 2764 4192 1802
Fragments arranged by size:
4192 2764 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3971 4034 4428
Size: 54 1425 50 275 18 2141 63 394
Cuts at: 4428 4940 5158 6712 7601 9230 8
Size: 512 218 1554 889 1629 43
Fragments arranged by size:
2141 1629 1554 1425 889 512 394 275
218 63 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6712 1537
Size: 275 4900 4090
Fragments arranged by size:
4900 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2436 2738 3552 6135 6393 7195 1353
Size: 1083 302 814 2583 258 802 3423
Fragments arranged by size:
3423 2583 1083 814 802 302 258
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7852 8226 8372 387
Size: 1064 138 6263 374 146 1280
Fragments arranged by size:
6263 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5635 5635
Size: 9265
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3087 3646 3723 4037 4173 4565 4775
Size: 393 1455 559 77 314 136 392 210
Cuts at: 4775 5247 5770 6159 6250 6426 6462 7328 7925
Size: 472 523 389 91 176 36 866 597
Cuts at: 7925 8712 8980 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1455 866 787 597 559 523 472
393 392 389 314 268 210 176 136
91 77 36
EcoNI CCTnn'n_nnAGG
Cuts at: 2571 4793 5159 2571
Size: 2222 366 6677
Fragments arranged by size:
6677 2222 366
EcoO109I rG'GnC_Cy
Cuts at: 2887 3718 6002 2887
Size: 831 2284 6150
Fragments arranged by size:
6150 2284 831
EcoRI G'AATT_C
Cuts at: 4643 6574 6952 4643
Size: 1931 378 6956
Fragments arranged by size:
6956 1931 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2624 2624
Size: 9265
FspI TGC'GCA
Cuts at: 7771 7771
Size: 9265
HaeII r_GCGC'y
Cuts at: 1678 3916 6160 8412 1678
Size: 2238 2244 2252 2531
Fragments arranged by size:
2531 2252 2244 2238
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2914 2946 3314 3346 3410
Size: 32 135 32 1342 32 368 32 64
Cuts at: 3410 3442 3870 3902 3952 3984 4370 4402 4939
Size: 32 428 32 50 32 386 32 537
Cuts at: 4939 4971 5708 5740 7906 7938 7980 8012 1373
Size: 32 737 32 2166 32 42 32 2626
Fragments arranged by size:
2626 2166 1342 737 537 428 386 368
135 64 50 42 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2350 4030 6609 6855 669
Size: 988 281 412 1680 2579 246 3079
Fragments arranged by size:
3079 2579 1680 988 412 281 246
HindIII A'AGCT_T
Cuts at: 748 2771 3240 748
Size: 2023 469 6773
Fragments arranged by size:
6773 2023 469
HpaI GTT'AAC
Cuts at: 6855 6855
Size: 9265
KpnI G_GTAC'C
Cuts at: 6590 6590
Size: 9265
MfeI C'AATT_G
Cuts at: 6864 6864
Size: 9265
MluI A'CGCG_T
Cuts at: 9141 9141
Size: 9265
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3112 4051 4186 4937 5906 8379 8563 1305
Size: 1807 939 135 751 969 2473 184 2007
Fragments arranged by size:
2473 2007 1807 969 939 751 184 135
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4430 10
Size: 54 1425 343 2598 4845
Fragments arranged by size:
4845 2598 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3684 5036 5949 7223 7582 7741 518
Size: 648 2518 1352 913 1274 359 159 2042
Fragments arranged by size:
2518 2042 1352 1274 913 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4252 4552 6173 6179 6358 6681 7358 8506
Size: 2646 300 1621 6 179 323 677 1148
Cuts at: 8506 8751 1606
Size: 245 2120
Fragments arranged by size:
2646 2120 1621 1148 677 323 300 245
179 6
NaeI GCC'GGC
Cuts at: 5049 5049
Size: 9265
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 7756
Fragments arranged by size:
7756 957 552
NdeI CA'TA_TG
Cuts at: 387 3517 387
Size: 3130 6135
NgoMIV G'CCGG_C
Cuts at: 5047 5047
Size: 9265
NheI G'CTAG_C
Cuts at: 1052 4093 1052
Size: 3041 6224
NotI GC'GGCC_GC
Cuts at: 6712 6712
Size: 9265
NspI r_CATG'y
Cuts at: 1132 1229 3056 3152 4184 5108 5804 6397 6623
Size: 97 1827 96 1032 924 696 593 226
Cuts at: 6623 8168 1132
Size: 1545 2229
Fragments arranged by size:
2229 1827 1545 1032 924 696 593 226
97 96
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9265
PciI A'CATG_T
Cuts at: 8164 8164
Size: 9265
PflMI CCAn_nnn'nTGG
Cuts at: 1108 6081 6116 1108
Size: 4973 35 4257
Fragments arranged by size:
4973 4257 35
PfoI T'CCnGG_A
Cuts at: 2799 3076 3828 3956 4701 2799
Size: 277 752 128 745 7363
Fragments arranged by size:
7363 752 745 277 128
PmeI GTTT'AAAC
Cuts at: 6569 6569
Size: 9265
PmlI CAC'GTG
Cuts at: 2653 2653
Size: 9265
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7334 7366 8847 8879 7334
Size: 32 1481 32 7720
Fragments arranged by size:
7720 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2887 3718 6002 2887
Size: 831 2284 6150
Fragments arranged by size:
6150 2284 831
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9265
PsiI TTA'TAA
Cuts at: 4656 6835 4656
Size: 2179 7086
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3021 3053 5790 5822 3021
Size: 32 2737 32 6464
Fragments arranged by size:
6464 2737 32 32
PstI C_TGCA'G
Cuts at: 830 2238 2440 2686 2715 5310 5674 5939 6178
Size: 1408 202 246 29 2595 364 265 239
Cuts at: 6178 6617 830
Size: 439 3478
Fragments arranged by size:
3478 2595 1408 439 364 265 246 239
202 29
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9265
PvuII CAG'CTG
Cuts at: 4252 4552 6173 6179 6358 4252
Size: 300 1621 6 179 7159
Fragments arranged by size:
7159 1621 300 179 6
SacI G_AGCT'C
Cuts at: 5637 5637
Size: 9265
SalI G'TCGA_C
Cuts at: 1936 6607 1936
Size: 4671 4594
SapI GCTCTTCn'nnn_
Cuts at: 2738 3552 6135 2738
Size: 814 2583 5868
Fragments arranged by size:
5868 2583 814
SbfI CC_TGCA'GG
Cuts at: 6617 6617
Size: 9265
ScaI AGT'ACT
Cuts at: 2973 5352 5808 6439 7513 9225 2973
Size: 2379 456 631 1074 1712 3013
Fragments arranged by size:
3013 2379 1712 1074 631 456
SfcI C'TryA_G
Cuts at: 826 1045 2234 2436 2682 2711 5306 5660 5670
Size: 219 1189 202 246 29 2595 354 10
Cuts at: 5670 5935 6174 6367 6613 7748 8429 8620 826
Size: 265 239 193 246 1135 681 191 1471
Fragments arranged by size:
2595 1471 1189 1135 681 354 265 246
246 239 219 202 193 191 29 10
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9265
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9265
SmaI CCC'GGG
Cuts at: 1872 6592 1872
Size: 4720 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2365 3392 3889 7369 8270 8532
Size: 197 926 422 1027 497 3480 901 262
Cuts at: 8532 820
Size: 1553
Fragments arranged by size:
3480 1553 1027 926 901 497 422 262
197
SnaBI TAC'GTA
Cuts at: 493 6562 7056 493
Size: 6069 494 2702
Fragments arranged by size:
6069 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9265
SphI G_CATG'C
Cuts at: 1132 5108 6623 1132
Size: 3976 1515 3774
Fragments arranged by size:
3976 3774 1515
SspI AAT'ATT
Cuts at: 5 52 4841 6013 6025 6454 7189 5
Size: 47 4789 1172 12 429 735 2081
Fragments arranged by size:
4789 2081 1172 735 429 47 12
StuI AGG'CCT
Cuts at: 1517 2447 2814 3623 3808 5846 1517
Size: 930 367 809 185 2038 4936
Fragments arranged by size:
4936 2038 930 809 367 185
StyI C'CwwG_G
Cuts at: 513 1065 2022 2044 3809 4746 4943 6704 513
Size: 552 957 22 1765 937 197 1761 3074
Fragments arranged by size:
3074 1765 1761 957 937 552 197 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3874 4692 4778 6513 7292 7309 7462
Size: 1255 2294 818 86 1735 779 17 153
Cuts at: 7462 7647 8092 325
Size: 185 445 1498
Fragments arranged by size:
2294 1735 1498 1255 818 779 445 185
153 86 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2785 2971 3921
Size: 274 80 33 51 1443 808 186 950
Cuts at: 3921 5350 5806 6437 6445 7511 9223 96
Size: 1429 456 631 8 1066 1712 138
Fragments arranged by size:
1712 1443 1429 1066 950 808 631 456
274 186 138 80 51 33 8
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2412 2444 3112 3144 4200 4232 5080 5112 9225
Size: 32 668 32 1056 32 848 32 4113
Cuts at: 9225 9257 2412
Size: 32 2420
Fragments arranged by size:
4113 2420 1056 848 668 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5720 1316
Size: 4404 4861
XbaI T'CTAG_A
Cuts at: 6601 6601
Size: 9265
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2420 4202 5241 2420
Size: 1782 1039 6444
Fragments arranged by size:
6444 1782 1039
XhoI C'TCGA_G
Cuts at: 1943 3889 1943
Size: 1946 7319
XmaI C'CCGG_G
Cuts at: 1870 6590 1870
Size: 4720 4545
XmnI GAAnn'nnTTC
Cuts at: 4104 4875 6089 7394 4104
Size: 771 1214 1305 5975
Fragments arranged by size:
5975 1305 1214 771
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7073 276
Size: 53 83 186 936 5539 2468
Fragments arranged by size:
5539 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BsmBI DraIII EcoRV FseI FspAI KasI NarI
NruI NsiI PacI PspOMI PspXI RsrII SacII SanDI
SexAI SfiI SfoI SrfI SwaI