Restriction map for
pFN21ASDA1334
(Circular) (Six-base) MAPSORT of: pFN21ASDA1334.seq Check: 5568 from: 1 to: 7669
pFN21ASDA1334 7669 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5479 278
Size: 53 83 186 936 3943 2468
Fragments arranged by size:
3943 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5012 1937
Size: 3075 4594
Acc65I G'GTAC_C
Cuts at: 4372 4990 4372
Size: 618 7051
AclI AA'CG_TT
Cuts at: 1563 5796 6169 1563
Size: 4233 373 3063
Fragments arranged by size:
4233 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2324 3066 3387 4940 5732 7116 7384 1632
Size: 692 742 321 1553 792 1384 268 1917
Fragments arranged by size:
1917 1553 1384 792 742 692 321 268
AflII C'TTAA_G
Cuts at: 820 1017 4283 820
Size: 197 3266 4206
Fragments arranged by size:
4206 3266 197
AflIII A'CryG_T
Cuts at: 1203 2215 4089 6568 7545 1203
Size: 1012 1874 2479 977 1327
Fragments arranged by size:
2479 1874 1327 1012 977
AgeI A'CCGG_T
Cuts at: 6500 6500
Size: 7669
AhdI GACnn_n'nnGTC
Cuts at: 6398 6398
Size: 7669
AleI CACnn'nnGTG
Cuts at: 1166 2172 1166
Size: 1006 6663
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7669
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4709 4741 4709
Size: 32 7637
AlwNI CAG_nnn'CTG
Cuts at: 3020 3322 3637 4774 5412 6984 3020
Size: 302 315 1137 638 1572 3705
Fragments arranged by size:
3705 1572 1137 638 315 302
ApaI G_GGCC'C
Cuts at: 3209 3209
Size: 7669
ApaLI G'TGCA_C
Cuts at: 5726 6882 5726
Size: 1156 6513
ApoI r'AATT_y
Cuts at: 1493 2718 3312 4586 4978 5204 5356 7465 1493
Size: 1225 594 1274 392 226 152 2109 1697
Fragments arranged by size:
2109 1697 1274 1225 594 392 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6223 160
Size: 872 5191 1606
Fragments arranged by size:
5191 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7669
AvaI C'yCGr_G
Cuts at: 1870 1943 4994 1870
Size: 73 3051 4545
Fragments arranged by size:
4545 3051 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4364 4397 4364
Size: 33 7636
BamHI G'GATC_C
Cuts at: 4999 5366 4999
Size: 367 7302
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4372 4990 6445 618
Size: 325 217 627 2585 618 1455 1842
Fragments arranged by size:
2585 1842 1455 627 618 325 217
BanII G_rGCy'C
Cuts at: 1391 2453 3209 3365 4640 4988 1391
Size: 1062 756 156 1275 348 4072
Fragments arranged by size:
4072 1275 1062 756 348 156
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4374 928
Size: 971 2475 4223
Fragments arranged by size:
4223 2475 971
BbvCI CC'TCA_GC
Cuts at: 3464 3596 3903 3464
Size: 132 307 7230
Fragments arranged by size:
7230 307 132
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5860 5894 1903
Size: 34 3923 34 3678
Fragments arranged by size:
3923 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2669 5561 6771 2669
Size: 2892 1210 3567
Fragments arranged by size:
3567 2892 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7669
BfrBI ATG'CAT
Cuts at: 2325 3647 4867 2325
Size: 1322 1220 5127
Fragments arranged by size:
5127 1322 1220
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6280 136
Size: 107 122 71 631 605 4608 1525
Fragments arranged by size:
4608 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2520 3061 3340 7664 2520
Size: 541 279 4324 2525
Fragments arranged by size:
4324 2525 541 279
BlpI GC'TnA_GC
Cuts at: 3993 5086 3993
Size: 1093 6576
Bme1580I G_kGCm'C
Cuts at: 1790 3209 5730 6886 1790
Size: 1419 2521 1156 2573
Fragments arranged by size:
2573 2521 1419 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7669
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2866 5400 6358
Size: 416 53 83 399 24 1449 2534 958
Cuts at: 6358 442
Size: 1753
Fragments arranged by size:
2534 1753 1449 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7669
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3472 4038 3472
Size: 566 7103
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4019 4346 4382 4670 4790 4796 6329 1239
Size: 2780 327 36 288 120 6 1533 2579
Fragments arranged by size:
2780 2579 1533 327 288 120 36 6
Bpu10I CC'TnA_GC
Cuts at: 2406 3167 3464 3596 3903 4599 2406
Size: 761 297 132 307 696 5476
Fragments arranged by size:
5476 761 696 307 297 132
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5794 6659 6957 5794
Size: 865 298 6506
Fragments arranged by size:
6506 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4510 6332 882
Size: 617 3011 1822 2219
Fragments arranged by size:
3011 2219 1822 617
BsaAI yAC'GTr
Cuts at: 493 1204 2218 4966 5460 493
Size: 711 1014 2748 494 2702
Fragments arranged by size:
2748 2702 1014 711 494
BsaBI GATnn'nnATC
Cuts at: 7663 7663
Size: 7669
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5476 5858 6493 275
Size: 53 83 186 936 3943 382 635 1451
Fragments arranged by size:
3943 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2276 6102 6500 6774 6921 7332 2276
Size: 3826 398 274 147 411 2613
Fragments arranged by size:
3826 2613 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2538 2568 2896 2926 4709
Size: 30 165 30 1135 30 328 30 1783
Cuts at: 4709 4739 1178
Size: 30 4108
Fragments arranged by size:
4108 1783 1135 328 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3515 1186
Size: 2329 5340
BseYI C'CCAG_C
Cuts at: 2353 4164 4728 6872 7368 2353
Size: 1811 564 2144 496 2654
Fragments arranged by size:
2654 2144 1811 564 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2447 2667 3548 4904 1163
Size: 573 711 220 881 1356 3928
Fragments arranged by size:
3928 1356 881 711 573 220
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5119 5880 6908 1540
Size: 275 186 3118 761 1028 2301
Fragments arranged by size:
3118 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3365 4640 5730 5815 6886 3365
Size: 1275 1090 85 1071 4148
Fragments arranged by size:
4148 1275 1090 1071 85
BsmI GAATG_Cn'
Cuts at: 2228 2327 3649 4829 4869 5180 5273 2228
Size: 99 1322 1180 40 311 93 4624
Fragments arranged by size:
4624 1322 1180 311 99 93 40
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2453 3209 3365 4640 4988 5730 5815
Size: 399 663 756 156 1275 348 742 85
Cuts at: 5815 6886 1391
Size: 1071 2174
Fragments arranged by size:
2174 1275 1071 756 742 663 399 348
156 85
BspEI T'CCGG_A
Cuts at: 7332 7332
Size: 7669
BspHI T'CATG_A
Cuts at: 2235 3846 5558 7288 2235
Size: 1611 1712 1730 2616
Fragments arranged by size:
2616 1730 1712 1611
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5024 844
Size: 4180 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5556 1394
Size: 429 3733 3507
Fragments arranged by size:
3733 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4374 6164 6338 65
Size: 1183 3126 1790 174 1396
Fragments arranged by size:
3126 1790 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2085 6313 6500 1700
Size: 110 275 4228 187 2869
Fragments arranged by size:
4228 2869 275 187 110
BsrGI T'GTAC_A
Cuts at: 96 2500 3975 96
Size: 2404 1475 3790
Fragments arranged by size:
3790 2404 1475
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7669
BssSI C'ACGA_G
Cuts at: 3590 5729 6741 3590
Size: 2139 1012 4518
Fragments arranged by size:
4518 2139 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3089 3637 4112 794
Size: 2295 548 475 4351
Fragments arranged by size:
4351 2295 548 475
BstBI TT'CG_AA
Cuts at: 2007 6473 2007
Size: 4466 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7669
BstYI r'GATC_y
Cuts at: 1972 2520 3061 3340 3634 4999 5366 5751 5768
Size: 548 541 279 294 1365 367 385 17
Cuts at: 5768 7220 7306 7318 7664 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1365 548 541 385 367 346
294 279 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2228 2228
Size: 7669
BtgI C'CryG_G
Cuts at: 513 1065 2100 513
Size: 552 1035 6082
Fragments arranged by size:
6082 1035 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6930
Fragments arranged by size:
6930 683 56
BtsI GCAGTG_nn'
Cuts at: 2132 5185 5979 5999 2132
Size: 3053 794 20 3802
Fragments arranged by size:
3802 3053 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7669
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7634
DraI TTT'AAA
Cuts at: 2763 4973 2763
Size: 2210 5459
DraIII CAC_nnn'GTG
Cuts at: 2249 2249
Size: 7669
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3305 4090 6676 809
Size: 507 1989 785 2586 1802
Fragments arranged by size:
2586 1989 1802 785 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5116 6005 7634
Size: 54 1425 50 275 18 3286 889 1629
Cuts at: 7634 8
Size: 43
Fragments arranged by size:
3286 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5116 1537
Size: 275 3304 4090
Fragments arranged by size:
4090 3304 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2021 2045 2893 3344 3603 3738 4007 4338
Size: 668 24 848 451 259 135 269 331
Cuts at: 4338 4645 5599 1353
Size: 307 954 3423
Fragments arranged by size:
3423 954 848 668 451 331 307 269
259 135 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3170 4835 6256 6630 6776 387
Size: 1064 138 1581 1665 1421 374 146 1280
Fragments arranged by size:
1665 1581 1421 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3363 4638 3363
Size: 1275 6394
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2324 3066 3387 4019 4346 4382 4670
Size: 393 692 742 321 632 327 36 288
Cuts at: 4670 4790 4796 4940 5732 6329 7116 7384 1239
Size: 120 6 144 792 597 787 268 1524
Fragments arranged by size:
1524 792 787 742 692 632 597 393
327 321 288 268 144 120 36 6
EcoRI G'AATT_C
Cuts at: 2718 3312 4978 5356 2718
Size: 594 1666 378 5031
Fragments arranged by size:
5031 1666 594 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3536 4729 3536
Size: 1193 6476
FspI TGC'GCA
Cuts at: 6175 6175
Size: 7669
HaeII r_GCGC'y
Cuts at: 1678 2289 4784 6816 1678
Size: 611 2495 2032 2531
Fragments arranged by size:
2531 2495 2032 611
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2538 2570 2783 2815 3053
Size: 32 135 32 966 32 213 32 238
Cuts at: 3053 3085 3236 3268 4103 4135 4835 4867 6310
Size: 32 151 32 835 32 700 32 1443
Cuts at: 6310 6342 6384 6416 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1443 966 835 700 238 213 151
135 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2368 4161 5013 5259 669
Size: 988 281 430 1793 852 246 3079
Fragments arranged by size:
3079 1793 988 852 430 281 246
HindIII A'AGCT_T
Cuts at: 748 3169 748
Size: 2421 5248
HpaI GTT'AAC
Cuts at: 5259 5259
Size: 7669
KpnI G_GTAC'C
Cuts at: 4376 4994 4376
Size: 618 7051
MfeI C'AATT_G
Cuts at: 4079 5268 4079
Size: 1189 6480
MluI A'CGCG_T
Cuts at: 7545 7545
Size: 7669
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6783 6967 1305
Size: 5478 184 2007
Fragments arranged by size:
5478 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5847
Fragments arranged by size:
5847 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2172 5627 5986 6145 518
Size: 648 1006 3455 359 159 2042
Fragments arranged by size:
3455 2042 1006 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2402 2488 3973 4040 4774 4816 5085 5762
Size: 796 86 1485 67 734 42 269 677
Cuts at: 5762 6910 7155 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1485 1148 796 734 677 269 245
86 67 42
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7117
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7669
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7669
NotI GC'GGCC_GC
Cuts at: 5116 5116
Size: 7669
NsiI A_TGCA'T
Cuts at: 2327 3649 4869 2327
Size: 1322 1220 5127
Fragments arranged by size:
5127 1322 1220
NspI r_CATG'y
Cuts at: 1132 1229 5027 6572 1132
Size: 97 3798 1545 2229
Fragments arranged by size:
3798 2229 1545 97
PacI TTA_AT'TAA
Cuts at: 2637 2637
Size: 7669
PasI CC'CwG_GG
Cuts at: 1348 3214 1348
Size: 1866 5803
PciI A'CATG_T
Cuts at: 6568 6568
Size: 7669
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2067 1108
Size: 959 6710
PfoI T'CCnGG_A
Cuts at: 2535 2561 2535
Size: 26 7643
PmeI GTTT'AAAC
Cuts at: 4973 4973
Size: 7669
PmlI CAC'GTG
Cuts at: 2218 2218
Size: 7669
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4190 4222 4709 4741 5738 5770 7251 7283 4190
Size: 32 487 32 997 32 1481 32 4576
Fragments arranged by size:
4576 1481 997 487 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7669
PsiI TTA'TAA
Cuts at: 5239 5239
Size: 7669
PspOMI G'GGCC_C
Cuts at: 3205 3205
Size: 7669
PstI C_TGCA'G
Cuts at: 830 2097 3642 4252 4788 5021 830
Size: 1267 1545 610 536 233 3478
Fragments arranged by size:
3478 1545 1267 610 536 233
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7669
PvuII CAG'CTG
Cuts at: 3973 4040 4774 4816 3973
Size: 67 734 42 6826
Fragments arranged by size:
6826 734 67 42
SacI G_AGCT'C
Cuts at: 3365 4640 3365
Size: 1275 6394
SalI G'TCGA_C
Cuts at: 1936 5011 1936
Size: 3075 4594
SapI GCTCTTCn'nnn_
Cuts at: 2021 4645 2021
Size: 2624 5045
SbfI CC_TGCA'GG
Cuts at: 5021 5021
Size: 7669
ScaI AGT'ACT
Cuts at: 3010 4917 5917 7629 3010
Size: 1907 1000 1712 3050
Fragments arranged by size:
3050 1907 1712 1000
SexAI A'CCwGG_T
Cuts at: 3223 3223
Size: 7669
SfcI C'TryA_G
Cuts at: 826 1045 2093 3012 3344 3386 3638 4248 4784
Size: 219 1048 919 332 42 252 610 536
Cuts at: 4784 5017 6152 6833 7024 826
Size: 233 1135 681 191 1471
Fragments arranged by size:
1471 1135 1048 919 681 610 536 332
252 233 219 191 42
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7669
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7669
SmaI CCC'GGG
Cuts at: 1872 4996 1872
Size: 3124 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4283 5773 6674 6936 820
Size: 197 926 2340 1490 901 262 1553
Fragments arranged by size:
2340 1553 1490 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4966 5460 493
Size: 4473 494 2702
Fragments arranged by size:
4473 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7669
SphI G_CATG'C
Cuts at: 1132 5027 1132
Size: 3895 3774
SspI AAT'ATT
Cuts at: 5 52 4534 5593 5
Size: 47 4482 1059 2081
Fragments arranged by size:
4482 2081 1059 47
StuI AGG'CCT
Cuts at: 1517 4712 1517
Size: 3195 4474
StyI C'CwwG_G
Cuts at: 513 1065 3147 5108 513
Size: 552 2082 1961 3074
Fragments arranged by size:
3074 2082 1961 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3160 3206 3536 4368 5696 5713 5866
Size: 1255 1580 46 330 832 1328 17 153
Cuts at: 5866 6051 6496 325
Size: 185 445 1498
Fragments arranged by size:
1580 1498 1328 1255 832 445 330 185
153 46 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2500 3008 3975
Size: 274 80 33 51 1443 523 508 967
Cuts at: 3975 4915 5915 7627 96
Size: 940 1000 1712 138
Fragments arranged by size:
1712 1443 1000 967 940 523 508 274
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2557 2589 7629 7661 2557
Size: 32 5040 32 2565
Fragments arranged by size:
5040 2565 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2795 1316
Size: 1479 6190
XbaI T'CTAG_A
Cuts at: 4024 5005 4024
Size: 981 6688
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7669
XmaI C'CCGG_G
Cuts at: 1870 4994 1870
Size: 3124 4545
XmnI GAAnn'nnTTC
Cuts at: 4533 5798 4533
Size: 1265 6404
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5477 276
Size: 53 83 186 936 3943 2468
Fragments arranged by size:
3943 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BbeI BsiWI BsmBI BstXI
BstZ17I EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI PpuMI PspXI PsrI RsrII SacII
SanDI SfiI SfoI SrfI SwaI XcmI