Restriction map for
pFN21ASDA1293
(Circular) (Six-base) MAPSORT of: pFN21ASDA1293.seq Check: 5520 from: 1 to: 5986
pFN21ASDA1293 5986 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3796 278
Size: 53 83 186 936 2260 2468
Fragments arranged by size:
2468 2260 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3329 1937
Size: 1392 4594
Acc65I G'GTAC_C
Cuts at: 2136 3307 2136
Size: 1171 4815
AclI AA'CG_TT
Cuts at: 1563 4113 4486 1563
Size: 2550 373 3063
Fragments arranged by size:
3063 2550 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2669 3098 4049 5433 5701 1632
Size: 1037 429 951 1384 268 1917
Fragments arranged by size:
1917 1384 1037 951 429 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5789
AflIII A'CryG_T
Cuts at: 1203 4885 5862 1203
Size: 3682 977 1327
Fragments arranged by size:
3682 1327 977
AgeI A'CCGG_T
Cuts at: 4817 4817
Size: 5986
AhdI GACnn_n'nnGTC
Cuts at: 4715 4715
Size: 5986
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5986
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2916 1184
Size: 1732 4254
AlwNI CAG_nnn'CTG
Cuts at: 2737 3043 3076 3729 5301 2737
Size: 306 33 653 1572 3422
Fragments arranged by size:
3422 1572 653 306 33
ApaLI G'TGCA_C
Cuts at: 2129 4043 5199 2129
Size: 1914 1156 2916
Fragments arranged by size:
2916 1914 1156
ApoI r'AATT_y
Cuts at: 1493 2236 3295 3521 3673 5782 1493
Size: 743 1059 226 152 2109 1697
Fragments arranged by size:
2109 1697 1059 743 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4540 160
Size: 872 3508 1606
Fragments arranged by size:
3508 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5986
AvaI C'yCGr_G
Cuts at: 1870 1943 2088 2196 3117 3311 1870
Size: 73 145 108 921 194 4545
Fragments arranged by size:
4545 921 194 145 108 73
BamHI G'GATC_C
Cuts at: 3316 3683 3316
Size: 367 5619
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2083 2136 2172 2322 3307
Size: 325 217 627 296 53 36 150 985
Cuts at: 3307 4762 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 985 627 325 296 217 150
53 36
BanII G_rGCy'C
Cuts at: 1391 2203 2697 3305 1391
Size: 812 494 608 4072
Fragments arranged by size:
4072 812 608 494
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2050 3183 928
Size: 971 151 1133 3731
Fragments arranged by size:
3731 1133 971 151
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2187 2221 4177 4211 1903
Size: 34 250 34 1956 34 3678
Fragments arranged by size:
3678 1956 250 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3878 5088 3878
Size: 1210 4776
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5986
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4597 136
Size: 107 122 71 631 605 2925 1525
Fragments arranged by size:
2925 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2045 2565 5981 2045
Size: 520 3416 2050
Fragments arranged by size:
3416 2050 520
BlpI GC'TnA_GC
Cuts at: 3403 3403
Size: 5986
Bme1580I G_kGCm'C
Cuts at: 1790 2086 2133 2164 2325 4047 5203 1790
Size: 296 47 31 161 1722 1156 2573
Fragments arranged by size:
2573 1722 1156 296 161 47 31
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5986
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2089 2135 2970
Size: 416 53 83 399 24 672 46 835
Cuts at: 2970 3717 4675 442
Size: 747 958 1753
Fragments arranged by size:
1753 958 835 747 672 416 399 83
53 46 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5986
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2387 2450 2939 3042 4646 1239
Size: 1148 63 489 103 1604 2579
Fragments arranged by size:
2579 1604 1148 489 103 63
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2339 4111 4976 5274 2339
Size: 1772 865 298 3051
Fragments arranged by size:
3051 1772 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2223 4649 882
Size: 617 724 2426 2219
Fragments arranged by size:
2426 2219 724 617
BsaAI yAC'GTr
Cuts at: 493 1204 3283 3777 493
Size: 711 2079 494 2702
Fragments arranged by size:
2702 2079 711 494
BsaBI GATnn'nnATC
Cuts at: 5980 5980
Size: 5986
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3793 4175 4810 275
Size: 53 83 186 936 2260 382 635 1451
Fragments arranged by size:
2260 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4419 4817 5091 5238 5649 4419
Size: 398 274 147 411 4756
Fragments arranged by size:
4756 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5761
Fragments arranged by size:
5761 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2200 2735 3152 1186
Size: 1014 535 417 4020
Fragments arranged by size:
4020 1014 535 417
BseYI C'CCAG_C
Cuts at: 2101 3232 5189 5685 2101
Size: 1131 1957 496 2402
Fragments arranged by size:
2402 1957 1131 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5413
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3436 4197 5225 1540
Size: 275 186 1435 761 1028 2301
Fragments arranged by size:
2301 1435 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2133 2697 2895 4047 4132 5203 2133
Size: 564 198 1152 85 1071 2916
Fragments arranged by size:
2916 1152 1071 564 198 85
BsmI GAATG_Cn'
Cuts at: 2420 3497 3590 2420
Size: 1077 93 4816
Fragments arranged by size:
4816 1077 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2086 2133 2164 2203 2325 2697 2895
Size: 399 296 47 31 39 122 372 198
Cuts at: 2895 3305 4047 4132 5203 1391
Size: 410 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 410 399 372 296 198
122 85 47 39 31
BspEI T'CCGG_A
Cuts at: 5649 5649
Size: 5986
BspHI T'CATG_A
Cuts at: 3875 5605 3875
Size: 1730 4256
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3341 844
Size: 2497 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3873 1394
Size: 429 2050 3507
Fragments arranged by size:
3507 2050 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2770 4481 4655 65
Size: 1183 1522 1711 174 1396
Fragments arranged by size:
1711 1522 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2984 4630 4817 1700
Size: 110 1174 1646 187 2869
Fragments arranged by size:
2869 1646 1174 187 110
BsrGI T'GTAC_A
Cuts at: 96 2862 96
Size: 2766 3220
BssHII G'CGCG_C
Cuts at: 1925 3254 1925
Size: 1329 4657
BssSI C'ACGA_G
Cuts at: 4046 5058 4046
Size: 1012 4974
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5986
BstBI TT'CG_AA
Cuts at: 2007 4790 2007
Size: 2783 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5986
BstXI CCAn_nnnn'nTGG
Cuts at: 2866 2866
Size: 5986
BstYI r'GATC_y
Cuts at: 1972 2045 2565 2770 2910 3072 3145 3316 3683
Size: 73 520 205 140 162 73 171 367
Cuts at: 3683 4068 4085 5537 5623 5635 5981 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 520 385 367 346 205 171
162 140 86 73 73 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5434
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5247
Fragments arranged by size:
5247 683 56
BtsI GCAGTG_nn'
Cuts at: 3162 3502 4296 4316 3162
Size: 340 794 20 4832
Fragments arranged by size:
4832 794 340 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5986
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5951
DraI TTT'AAA
Cuts at: 3290 3290
Size: 5986
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4993 809
Size: 507 3677 1802
Fragments arranged by size:
3677 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3433 4322 5951
Size: 54 1425 50 275 18 1603 889 1629
Cuts at: 5951 8
Size: 43
Fragments arranged by size:
1629 1603 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3433 1537
Size: 275 1621 4090
Fragments arranged by size:
4090 1621 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2702 3916 1353
Size: 1349 1214 3423
Fragments arranged by size:
3423 1349 1214
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4573 4947 5093 387
Size: 1064 138 2984 374 146 1280
Fragments arranged by size:
2984 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2695 2695
Size: 5986
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2387 2450 2669 2939 3042 3098 4049
Size: 393 755 63 219 270 103 56 951
Cuts at: 4049 4646 5433 5701 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 951 787 755 597 393 270 268
219 103 63 56
EcoO109I rG'GnC_Cy
Cuts at: 2362 2971 2362
Size: 609 5377
EcoRI G'AATT_C
Cuts at: 2236 3295 3673 2236
Size: 1059 378 4549
Fragments arranged by size:
4549 1059 378
FspI TGC'GCA
Cuts at: 4492 4492
Size: 5986
HaeII r_GCGC'y
Cuts at: 1678 3131 5133 1678
Size: 1453 2002 2531
Fragments arranged by size:
2531 2002 1453
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2698 2730 4627 4659 4701
Size: 32 135 32 1126 32 1897 32 42
Cuts at: 4701 4733 1373
Size: 32 2626
Fragments arranged by size:
2626 1897 1126 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3330 3576 669
Size: 988 281 1392 246 3079
Fragments arranged by size:
3079 1392 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2508 748
Size: 1760 4226
HpaI GTT'AAC
Cuts at: 3576 3576
Size: 5986
KpnI G_GTAC'C
Cuts at: 2140 3311 2140
Size: 1171 4815
MfeI C'AATT_G
Cuts at: 3585 3585
Size: 5986
MluI A'CGCG_T
Cuts at: 5862 5862
Size: 5986
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5100 5284 1305
Size: 3795 184 2007
Fragments arranged by size:
3795 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4164
Fragments arranged by size:
4164 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2864 2898 3944 4303 4462 518
Size: 648 1698 34 1046 359 159 2042
Fragments arranged by size:
2042 1698 1046 648 359 159 34
MspA1I CmG'CkG
Cuts at: 1606 2037 2166 2470 3024 3402 4079 5227 5472
Size: 431 129 304 554 378 677 1148 245
Cuts at: 5472 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 554 431 378 304 245
129
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5434
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5986
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5986
NotI GC'GGCC_GC
Cuts at: 3433 3433
Size: 5986
NspI r_CATG'y
Cuts at: 1132 1229 2634 3344 4889 1132
Size: 97 1405 710 1545 2229
Fragments arranged by size:
2229 1545 1405 710 97
PasI CC'CwG_GG
Cuts at: 1348 2758 1348
Size: 1410 4576
PciI A'CATG_T
Cuts at: 4885 4885
Size: 5986
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3196 1108
Size: 2088 3898
PfoI T'CCnGG_A
Cuts at: 2364 2424 2622 3006 2364
Size: 60 198 384 5344
Fragments arranged by size:
5344 384 198 60
PmeI GTTT'AAAC
Cuts at: 3290 3290
Size: 5986
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4055 4087 5568 5600 4055
Size: 32 1481 32 4441
Fragments arranged by size:
4441 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2362 2971 2362
Size: 609 5377
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5986
PsiI TTA'TAA
Cuts at: 3556 3556
Size: 5986
PspXI vC'TCGA_Gb
Cuts at: 2196 2196
Size: 5986
PstI C_TGCA'G
Cuts at: 830 2706 2857 3338 830
Size: 1876 151 481 3478
Fragments arranged by size:
3478 1876 481 151
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5986
PvuII CAG'CTG
Cuts at: 3024 3024
Size: 5986
SacI G_AGCT'C
Cuts at: 2697 2697
Size: 5986
SalI G'TCGA_C
Cuts at: 1936 3328 1936
Size: 1392 4594
SanDI GG'GwC_CC
Cuts at: 2971 2971
Size: 5986
SapI GCTCTTCn'nnn_
Cuts at: 2702 2702
Size: 5986
SbfI CC_TGCA'GG
Cuts at: 3338 3338
Size: 5986
ScaI AGT'ACT
Cuts at: 4234 5946 4234
Size: 1712 4274
SfcI C'TryA_G
Cuts at: 826 1045 2702 2847 2853 3334 4469 5150 5341
Size: 219 1657 145 6 481 1135 681 191
Cuts at: 5341 826
Size: 1471
Fragments arranged by size:
1657 1471 1135 681 481 219 191 145
6
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5986
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5986
SmaI CCC'GGG
Cuts at: 1872 2090 3119 3313 1872
Size: 218 1029 194 4545
Fragments arranged by size:
4545 1029 218 194
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2196 2354 4090 4991 5253 820
Size: 197 926 253 158 1736 901 262 1553
Fragments arranged by size:
1736 1553 926 901 262 253 197 158
SnaBI TAC'GTA
Cuts at: 493 3283 3777 493
Size: 2790 494 2702
Fragments arranged by size:
2790 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5986
SphI G_CATG'C
Cuts at: 1132 3344 1132
Size: 2212 3774
SspI AAT'ATT
Cuts at: 5 52 3910 5
Size: 47 3858 2081
Fragments arranged by size:
3858 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5986
StyI C'CwwG_G
Cuts at: 513 1065 2441 3425 513
Size: 552 1376 984 3074
Fragments arranged by size:
3074 1376 984 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2340 4013 4030 4183 4368 4813 325
Size: 1255 760 1673 17 153 185 445 1498
Fragments arranged by size:
1673 1498 1255 760 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2370 2814 2862
Size: 274 80 33 51 1443 393 444 48
Cuts at: 2862 4232 5944 96
Size: 1370 1712 138
Fragments arranged by size:
1712 1443 1370 444 393 274 138 80
51 48 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5946 5978 5946
Size: 32 5954
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5986
XbaI T'CTAG_A
Cuts at: 3322 3322
Size: 5986
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2076 2148 2076
Size: 72 5914
XhoI C'TCGA_G
Cuts at: 1943 2196 1943
Size: 253 5733
XmaI C'CCGG_G
Cuts at: 1870 2088 3117 3311 1870
Size: 218 1029 194 4545
Fragments arranged by size:
4545 1029 218 194
XmnI GAAnn'nnTTC
Cuts at: 4115 4115
Size: 5986
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3794 276
Size: 53 83 186 936 2260 2468
Fragments arranged by size:
2468 2260 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI
PvuI PvuII SacI SalI SanDI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI
RsrII SacII SexAI SfiI SfoI SrfI SwaI