Restriction map for
pFN21ASDA1284
(Circular) (Six-base) MAPSORT of: pFN21ASDA1284.seq Check: 5594 from: 1 to: 7555
pFN21ASDA1284 7555 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2779 2779
Size: 7555
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5365 278
Size: 53 83 186 936 3829 2468
Fragments arranged by size:
3829 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3557 3642 4310 4898 1937
Size: 1620 85 668 588 4594
Fragments arranged by size:
4594 1620 668 588 85
Acc65I G'GTAC_C
Cuts at: 4876 4876
Size: 7555
AclI AA'CG_TT
Cuts at: 1563 5682 6055 1563
Size: 4119 373 3063
Fragments arranged by size:
4119 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3045 3219 3335 3780 3798 5618 7002 7270
Size: 1413 174 116 445 18 1820 1384 268
Cuts at: 7270 1632
Size: 1917
Fragments arranged by size:
1917 1820 1413 1384 445 268 174 116
18
AfeI AGC'GCT
Cuts at: 2402 2402
Size: 7555
AflII C'TTAA_G
Cuts at: 820 1017 4289 820
Size: 197 3272 4086
Fragments arranged by size:
4086 3272 197
AflIII A'CryG_T
Cuts at: 1203 6454 7431 1203
Size: 5251 977 1327
Fragments arranged by size:
5251 1327 977
AgeI A'CCGG_T
Cuts at: 6386 6386
Size: 7555
AhdI GACnn_n'nnGTC
Cuts at: 4401 6284 4401
Size: 1883 5672
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7555
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3417 3626 1184
Size: 2233 209 5113
Fragments arranged by size:
5113 2233 209
AlwNI CAG_nnn'CTG
Cuts at: 4619 5298 6870 4619
Size: 679 1572 5304
Fragments arranged by size:
5304 1572 679
ApaLI G'TGCA_C
Cuts at: 5612 6768 5612
Size: 1156 6399
ApoI r'AATT_y
Cuts at: 1493 2370 3000 3141 3257 4522 4864 5090 5242
Size: 877 630 141 116 1265 342 226 152
Cuts at: 5242 7351 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1265 877 630 342 226 152
141 116
AseI AT'TA_AT
Cuts at: 160 1032 6109 160
Size: 872 5077 1606
Fragments arranged by size:
5077 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7555
AvaI C'yCGr_G
Cuts at: 1870 1943 2134 4880 1870
Size: 73 191 2746 4545
Fragments arranged by size:
4545 2746 191 73
BamHI G'GATC_C
Cuts at: 4885 5252 4885
Size: 367 7188
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4876 6331 618
Size: 325 217 627 3089 1455 1842
Fragments arranged by size:
3089 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2060 2070 4259 4874 1391
Size: 669 10 2189 615 4072
Fragments arranged by size:
4072 2189 669 615 10
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2474 3564 4626 928
Size: 971 575 1090 1062 3857
Fragments arranged by size:
3857 1090 1062 971 575
BbvCI CC'TCA_GC
Cuts at: 2149 3723 4509 2149
Size: 1574 786 5195
Fragments arranged by size:
5195 1574 786
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5746 5780 1903
Size: 34 3809 34 3678
Fragments arranged by size:
3809 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2449 2924 5447 6657 2449
Size: 475 2523 1210 3347
Fragments arranged by size:
3347 2523 1210 475
BclI T'GATC_A
Cuts at: 1548 3699 4696 1548
Size: 2151 997 4407
Fragments arranged by size:
4407 2151 997
BfrBI ATG'CAT
Cuts at: 2813 2813
Size: 7555
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2033 2185 6166
Size: 107 122 71 631 605 361 152 3981
Cuts at: 6166 136
Size: 1525
Fragments arranged by size:
3981 1525 631 605 361 152 122 107
71
BglII A'GATC_T
Cuts at: 7550 7550
Size: 7555
BlpI GC'TnA_GC
Cuts at: 3390 4972 3390
Size: 1582 5973
Bme1580I G_kGCm'C
Cuts at: 1790 5616 6772 1790
Size: 3826 1156 2573
Fragments arranged by size:
3826 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7555
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3105 3378 4738
Size: 416 53 83 399 24 1688 273 1360
Cuts at: 4738 5286 6244 442
Size: 548 958 1753
Fragments arranged by size:
1753 1688 1360 958 548 416 399 273
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3962 1056
Size: 2906 4649
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2094 2629 3456 3864 3954 6215 1239
Size: 855 535 827 408 90 2261 2579
Fragments arranged by size:
2579 2261 855 827 535 408 90
Bpu10I CC'TnA_GC
Cuts at: 2149 3723 4509 2149
Size: 1574 786 5195
Fragments arranged by size:
5195 1574 786
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2195 2339 2543 3736 4749 5680 6545 6843 2195
Size: 144 204 1193 1013 931 865 298 2907
Fragments arranged by size:
2907 1193 1013 931 865 298 204 144
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2070 4599 6218 882
Size: 617 571 2529 1619 2219
Fragments arranged by size:
2529 2219 1619 617 571
BsaAI yAC'GTr
Cuts at: 493 1204 4016 4852 5346 493
Size: 711 2812 836 494 2702
Fragments arranged by size:
2812 2702 836 711 494
BsaBI GATnn'nnATC
Cuts at: 7549 7549
Size: 7555
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5362 5744 6379 275
Size: 53 83 186 936 3829 382 635 1451
Fragments arranged by size:
3829 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2618 3441 3769 4222 5988 6386 6660 6807 7218
Size: 823 328 453 1766 398 274 147 411
Cuts at: 7218 2618
Size: 2955
Fragments arranged by size:
2955 1766 823 453 411 398 328 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2466 2496 2889 2919 1178
Size: 30 165 30 1063 30 393 30 5814
Fragments arranged by size:
5814 1063 393 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2928 1186
Size: 1742 5813
BseYI C'CCAG_C
Cuts at: 6758 7254 6758
Size: 496 7059
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2219 3432 4572 1163
Size: 573 483 1213 1140 4146
Fragments arranged by size:
4146 1213 1140 573 483
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5005 5766 6794 1540
Size: 275 186 3004 761 1028 2301
Fragments arranged by size:
3004 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2060 2070 3349 3391 5616 5701 6772 2060
Size: 10 1279 42 2225 85 1071 2843
Fragments arranged by size:
2843 2225 1279 1071 85 42 10
BsmI GAATG_Cn'
Cuts at: 2772 3367 5066 5159 2772
Size: 595 1699 93 5168
Fragments arranged by size:
5168 1699 595 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2060 2070 3349 3391 4259 4874 5616
Size: 399 270 10 1279 42 868 615 742
Cuts at: 5616 5701 6772 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1279 1071 868 742 615 399 270
85 42 10
BspEI T'CCGG_A
Cuts at: 3769 7218 3769
Size: 3449 4106
BspHI T'CATG_A
Cuts at: 4786 5444 7174 4786
Size: 658 1730 5167
Fragments arranged by size:
5167 1730 658
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2779 4910 844
Size: 1935 2131 3489
Fragments arranged by size:
3489 2131 1935
BsrBI CCG'CTC
Cuts at: 1394 1823 2688 5442 1394
Size: 429 865 2754 3507
Fragments arranged by size:
3507 2754 865 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2428 6050 6224 65
Size: 1183 1180 3622 174 1396
Fragments arranged by size:
3622 1396 1183 1180 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6199 6386 1700
Size: 110 4389 187 2869
Fragments arranged by size:
4389 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7555
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7555
BssSI C'ACGA_G
Cuts at: 5615 6627 5615
Size: 1012 6543
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3515 794
Size: 2721 4834
BstBI TT'CG_AA
Cuts at: 2007 6359 2007
Size: 4352 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7555
BstXI CCAn_nnnn'nTGG
Cuts at: 2924 3929 2924
Size: 1005 6550
BstYI r'GATC_y
Cuts at: 1972 2672 4166 4885 5252 5637 5654 7106 7192
Size: 700 1494 719 367 385 17 1452 86
Cuts at: 7192 7204 7550 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1494 1452 719 700 385 367 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 3558 3643 3558
Size: 85 7470
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 6046
Fragments arranged by size:
6046 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6816
Fragments arranged by size:
6816 683 56
BtsI GCAGTG_nn'
Cuts at: 5071 5865 5885 5071
Size: 794 20 6741
Fragments arranged by size:
6741 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7555
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7520
DraI TTT'AAA
Cuts at: 3129 4825 4859 3129
Size: 1696 34 5825
Fragments arranged by size:
5825 1696 34
DraIII CAC_nnn'GTG
Cuts at: 3074 3074
Size: 7555
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6562 809
Size: 507 5246 1802
Fragments arranged by size:
5246 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5002 5891 7520
Size: 54 1425 50 275 18 3172 889 1629
Cuts at: 7520 8
Size: 43
Fragments arranged by size:
3172 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5002 1537
Size: 275 3190 4090
Fragments arranged by size:
4090 3190 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2089 2678 2895 2985 3216 3921 4320 4470
Size: 736 589 217 90 231 705 399 150
Cuts at: 4470 4572 5485 1353
Size: 102 913 3423
Fragments arranged by size:
3423 913 736 705 589 399 231 217
150 102 90
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6142 6516 6662 387
Size: 1064 138 4553 374 146 1280
Fragments arranged by size:
4553 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2058 2068 2058
Size: 10 7545
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2094 2629 3045 3219 3335 3456 3780
Size: 393 462 535 416 174 116 121 324
Cuts at: 3780 3798 3864 3954 5618 6215 7002 7270 1239
Size: 18 66 90 1664 597 787 268 1524
Fragments arranged by size:
1664 1524 787 597 535 462 416 393
324 268 174 121 116 90 66 18
EcoNI CCTnn'n_nnAGG
Cuts at: 4475 4475
Size: 7555
EcoO109I rG'GnC_Cy
Cuts at: 4281 4351 4281
Size: 70 7485
EcoRI G'AATT_C
Cuts at: 4864 5242 4864
Size: 378 7177
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3227 3227
Size: 7555
FspI TGC'GCA
Cuts at: 3853 6061 3853
Size: 2208 5347
HaeII r_GCGC'y
Cuts at: 1678 2404 3378 6702 1678
Size: 726 974 3324 2531
Fragments arranged by size:
3324 2531 974 726
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2466 2498 2824 2856 3589
Size: 32 135 32 894 32 326 32 733
Cuts at: 3589 3621 4387 4419 6196 6228 6270 6302 1373
Size: 32 766 32 1777 32 42 32 2626
Fragments arranged by size:
2626 1777 894 766 733 326 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4842 4899 5145 669
Size: 988 281 2904 57 246 3079
Fragments arranged by size:
3079 2904 988 281 246 57
HindIII A'AGCT_T
Cuts at: 748 2561 2885 748
Size: 1813 324 5418
Fragments arranged by size:
5418 1813 324
HpaI GTT'AAC
Cuts at: 4842 5145 4842
Size: 303 7252
KpnI G_GTAC'C
Cuts at: 4880 4880
Size: 7555
MfeI C'AATT_G
Cuts at: 5154 5154
Size: 7555
MluI A'CGCG_T
Cuts at: 7431 7431
Size: 7555
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2509 2560 2889 3760 4115 4585 6669 6853
Size: 1204 51 329 871 355 470 2084 184
Cuts at: 6853 1305
Size: 2007
Fragments arranged by size:
2084 2007 1204 871 470 355 329 184
51
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5733
Fragments arranged by size:
5733 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5513 5872 6031 518
Size: 648 4347 359 159 2042
Fragments arranged by size:
4347 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2491 3925 4971 5648 6796 7041 1606
Size: 885 1434 1046 677 1148 245 2120
Fragments arranged by size:
2120 1434 1148 1046 885 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 6046
Fragments arranged by size:
6046 957 552
NdeI CA'TA_TG
Cuts at: 387 2815 3704 387
Size: 2428 889 4238
Fragments arranged by size:
4238 2428 889
NheI G'CTAG_C
Cuts at: 1052 3958 1052
Size: 2906 4649
NotI GC'GGCC_GC
Cuts at: 5002 5002
Size: 7555
NsiI A_TGCA'T
Cuts at: 2815 2815
Size: 7555
NspI r_CATG'y
Cuts at: 1132 1229 3082 4098 4913 6458 1132
Size: 97 1853 1016 815 1545 2229
Fragments arranged by size:
2229 1853 1545 1016 815 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7555
PciI A'CATG_T
Cuts at: 6454 6454
Size: 7555
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7555
PfoI T'CCnGG_A
Cuts at: 2776 3874 2776
Size: 1098 6457
PmeI GTTT'AAAC
Cuts at: 4859 4859
Size: 7555
PmlI CAC'GTG
Cuts at: 4016 4016
Size: 7555
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2337 2369 5624 5656 7137 7169 2337
Size: 32 3255 32 1481 32 2723
Fragments arranged by size:
3255 2723 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4281 4351 4281
Size: 70 7485
PshAI GACnn'nnGTC
Cuts at: 1613 2474 1613
Size: 861 6694
PsiI TTA'TAA
Cuts at: 5125 5125
Size: 7555
PstI C_TGCA'G
Cuts at: 830 3511 4907 830
Size: 2681 1396 3478
Fragments arranged by size:
3478 2681 1396
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7555
PvuII CAG'CTG
Cuts at: 3925 3925
Size: 7555
SacI G_AGCT'C
Cuts at: 2060 2070 2060
Size: 10 7545
SalI G'TCGA_C
Cuts at: 1936 4897 1936
Size: 2961 4594
SapI GCTCTTCn'nnn_
Cuts at: 2678 2985 2678
Size: 307 7248
SbfI CC_TGCA'GG
Cuts at: 4907 4907
Size: 7555
ScaI AGT'ACT
Cuts at: 3417 5803 7515 3417
Size: 2386 1712 3457
Fragments arranged by size:
3457 2386 1712
SfcI C'TryA_G
Cuts at: 826 1045 2963 3507 4153 4903 6038 6719 6910
Size: 219 1918 544 646 750 1135 681 191
Cuts at: 6910 826
Size: 1471
Fragments arranged by size:
1918 1471 1135 750 681 646 544 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7555
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7555
SmaI CCC'GGG
Cuts at: 1872 4882 1872
Size: 3010 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2174 2354 2558 3751 4289 4764
Size: 197 926 231 180 204 1193 538 475
Cuts at: 4764 5659 6560 6822 820
Size: 895 901 262 1553
Fragments arranged by size:
1553 1193 926 901 895 538 475 262
231 204 197 180
SnaBI TAC'GTA
Cuts at: 493 4852 5346 493
Size: 4359 494 2702
Fragments arranged by size:
4359 2702 494
SpeI A'CTAG_T
Cuts at: 152 3110 152
Size: 2958 4597
SphI G_CATG'C
Cuts at: 1132 4913 1132
Size: 3781 3774
SspI AAT'ATT
Cuts at: 5 52 5479 5
Size: 47 5427 2081
Fragments arranged by size:
5427 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7555
StyI C'CwwG_G
Cuts at: 513 1065 2022 3615 4994 513
Size: 552 957 1593 1379 3074
Fragments arranged by size:
3074 1593 1379 957 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5582 5599 5752 5937 6382 325
Size: 1255 4002 17 153 185 445 1498
Fragments arranged by size:
4002 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3415 3829 5801
Size: 274 80 33 51 1443 1438 414 1972
Cuts at: 5801 7513 96
Size: 1712 138
Fragments arranged by size:
1972 1712 1443 1438 414 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7515 7547 7515
Size: 32 7523
Tth111I GACn'n_nGTC
Cuts at: 1316 2860 4793 1316
Size: 1544 1933 4078
Fragments arranged by size:
4078 1933 1544
XbaI T'CTAG_A
Cuts at: 4891 4891
Size: 7555
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2029 2029
Size: 7555
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7555
XmaI C'CCGG_G
Cuts at: 1870 4880 1870
Size: 3010 4545
XmnI GAAnn'nnTTC
Cuts at: 3204 3717 3765 5684 3204
Size: 513 48 1919 5075
Fragments arranged by size:
5075 1919 513 48
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5363 276
Size: 53 83 186 936 3829 2468
Fragments arranged by size:
3829 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI
BsmBI Bsu36I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PspOMI PspXI PsrI RsrII SacII
SanDI SexAI SfiI SfoI SrfI SwaI