Restriction map for
pFN21ASDA1281
(Circular) (Six-base) MAPSORT of: pFN21ASDA1281.seq Check: 516 from: 1 to: 9265
pFN21ASDA1281 9265 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 6408 6496 6408
Size: 88 9177
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7075 278
Size: 53 83 186 936 5539 2468
Fragments arranged by size:
5539 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5703 5760 6608 1937
Size: 3766 57 848 4594
Fragments arranged by size:
4594 3766 848 57
Acc65I G'GTAC_C
Cuts at: 3735 6586 3735
Size: 2851 6414
AclI AA'CG_TT
Cuts at: 1563 7392 7765 1563
Size: 5829 373 3063
Fragments arranged by size:
5829 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2660 5585 7328 8712 8980 1632
Size: 1028 2925 1743 1384 268 1917
Fragments arranged by size:
2925 1917 1743 1384 1028 268
AfeI AGC'GCT
Cuts at: 2478 2478
Size: 9265
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9068
AflIII A'CryG_T
Cuts at: 1203 3083 8164 9141 1203
Size: 1880 5081 977 1327
Fragments arranged by size:
5081 1880 1327 977
AgeI A'CCGG_T
Cuts at: 3037 8096 3037
Size: 5059 4206
AhdI GACnn_n'nnGTC
Cuts at: 4882 5296 7994 4882
Size: 414 2698 6153
Fragments arranged by size:
6153 2698 414
AleI CACnn'nnGTG
Cuts at: 1166 4748 1166
Size: 3582 5683
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3862 5082 6528 1184
Size: 2678 1220 1446 3921
Fragments arranged by size:
3921 2678 1446 1220
AlwNI CAG_nnn'CTG
Cuts at: 5362 7008 8580 5362
Size: 1646 1572 6047
Fragments arranged by size:
6047 1646 1572
ApaI G_GGCC'C
Cuts at: 3126 3265 3126
Size: 139 9126
ApaLI G'TGCA_C
Cuts at: 7322 8478 7322
Size: 1156 8109
ApoI r'AATT_y
Cuts at: 1493 2116 2162 2273 2308 2470 3148 3598 3997
Size: 623 46 111 35 162 678 450 399
Cuts at: 3997 4150 6574 6800 6952 9061 1493
Size: 153 2424 226 152 2109 1697
Fragments arranged by size:
2424 2109 1697 678 623 450 399 226
162 153 152 111 46 35
AseI AT'TA_AT
Cuts at: 160 1032 4136 7819 160
Size: 872 3104 3683 1606
Fragments arranged by size:
3683 3104 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9265
AvaI C'yCGr_G
Cuts at: 1870 1943 2405 3355 4726 5713 5997 6448 6590
Size: 73 462 950 1371 987 284 451 142
Cuts at: 6590 1870
Size: 4545
Fragments arranged by size:
4545 1371 987 950 462 451 284 142
73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3852 3885 3852
Size: 33 9232
BamHI G'GATC_C
Cuts at: 2059 6595 6962 2059
Size: 4536 367 4362
Fragments arranged by size:
4536 4362 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2445 2621 2721 3735 3892
Size: 325 217 627 658 176 100 1014 157
Cuts at: 3892 4762 4771 5066 6396 6586 8041 618
Size: 870 9 295 1330 190 1455 1842
Fragments arranged by size:
1842 1455 1330 1014 870 658 627 325
295 217 190 176 157 100 9
BanII G_rGCy'C
Cuts at: 1391 2768 2847 3126 3265 3362 4416 5283 5421
Size: 1377 79 279 139 97 1054 867 138
Cuts at: 5421 5907 6584 1391
Size: 486 677 4072
Fragments arranged by size:
4072 1377 1054 867 677 486 279 139
138 97 79
BbeI G_GCGC'C
Cuts at: 2449 2625 2449
Size: 176 9089
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 8294
BbvCI CC'TCA_GC
Cuts at: 3483 4665 5205 3483
Size: 1182 540 7543
Fragments arranged by size:
7543 1182 540
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7456 7490 1903
Size: 34 5519 34 3678
Fragments arranged by size:
5519 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7157 8367 7157
Size: 1210 8055
BclI T'GATC_A
Cuts at: 1548 5220 1548
Size: 3672 5593
BfrBI ATG'CAT
Cuts at: 5273 5273
Size: 9265
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7876 136
Size: 107 122 71 631 605 6204 1525
Fragments arranged by size:
6204 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3804 9260 3804
Size: 5456 3809
BlpI GC'TnA_GC
Cuts at: 5622 6339 6682 5622
Size: 717 343 8205
Fragments arranged by size:
8205 717 343
Bme1580I G_kGCm'C
Cuts at: 1790 2724 2786 2833 3126 3265 4228 5071 5868
Size: 934 62 47 293 139 963 843 797
Cuts at: 5868 7326 8482 1790
Size: 1458 1156 2573
Fragments arranged by size:
2573 1458 1156 963 934 843 797 293
139 62 47
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9265
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2796 3268 4317
Size: 416 53 83 399 24 1379 472 1049
Cuts at: 4317 5397 6996 7954 442
Size: 1080 1599 958 1753
Fragments arranged by size:
1753 1599 1379 1080 1049 958 472 416
399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9265
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5859 5859
Size: 9265
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2215 2472 3320 3541 3654 3797 6144 6263
Size: 976 257 848 221 113 143 2347 119
Cuts at: 6263 7925 1239
Size: 1662 2579
Fragments arranged by size:
2579 2347 1662 976 848 257 221 143
119 113
Bpu10I CC'TnA_GC
Cuts at: 2257 3483 4665 5205 2257
Size: 1226 1182 540 6317
Fragments arranged by size:
6317 1226 1182 540
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3609 7390 8255 8553 3609
Size: 3781 865 298 4321
Fragments arranged by size:
4321 3781 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2315 7928 882
Size: 617 816 5613 2219
Fragments arranged by size:
5613 2219 816 617
BsaAI yAC'GTr
Cuts at: 493 1204 3207 3226 6562 7056 493
Size: 711 2003 19 3336 494 2702
Fragments arranged by size:
3336 2702 2003 711 494 19
BsaBI GATnn'nnATC
Cuts at: 9259 9259
Size: 9265
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2446 2622 7072 7454
Size: 53 83 186 936 913 176 4450 382
Cuts at: 7454 8089 275
Size: 635 1451
Fragments arranged by size:
4450 1451 936 913 635 382 186 176
83 53
BsaWI w'CCGG_w
Cuts at: 3037 5500 7698 8096 8370 8517 8928 3037
Size: 2463 2198 398 274 147 411 3374
Fragments arranged by size:
3374 2463 2198 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3539 3569 1178
Size: 30 165 30 2136 30 6874
Fragments arranged by size:
6874 2136 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2603 2894 2897 2900 2903 5409 5570 5915
Size: 1417 291 3 3 3 2506 161 345
Cuts at: 5915 5957 6010 1186
Size: 42 53 4441
Fragments arranged by size:
4441 2506 1417 345 291 161 53 42
3 3 3
BseYI C'CCAG_C
Cuts at: 2479 3989 4246 4429 4528 4703 5434 5867 6092
Size: 1510 257 183 99 175 731 433 225
Cuts at: 6092 8468 8964 2479
Size: 2376 496 2780
Fragments arranged by size:
2780 2376 1510 731 496 433 257 225
183 175 99
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3229 6386 1163
Size: 573 1493 3157 4042
Fragments arranged by size:
4042 3157 1493 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6715 7476 8504 1540
Size: 275 186 4714 761 1028 2301
Fragments arranged by size:
4714 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5096 5421 7326 7411 8482 5096
Size: 325 1905 85 1071 5879
Fragments arranged by size:
5879 1905 1071 325 85
BsiWI C'GTAC_G
Cuts at: 3222 3222
Size: 9265
BsmI GAATG_Cn'
Cuts at: 3851 4742 4957 6776 6869 3851
Size: 891 215 1819 93 6247
Fragments arranged by size:
6247 1819 891 215 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4493 4493
Size: 9265
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2724 2768 2786 2833 2847 3126 3265
Size: 399 934 44 18 47 14 279 139
Cuts at: 3265 3362 4228 4416 5071 5096 5283 5421 5868
Size: 97 866 188 655 25 187 138 447
Cuts at: 5868 5907 6584 7326 7411 8482 1391
Size: 39 677 742 85 1071 2174
Fragments arranged by size:
2174 1071 934 866 742 677 655 447
399 279 188 187 139 138 97 85
47 44 39 25 18 14
BspEI T'CCGG_A
Cuts at: 5500 8928 5500
Size: 3428 5837
BspHI T'CATG_A
Cuts at: 7154 8884 7154
Size: 1730 7535
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2679 4872 5050 6408 6496 6620 844
Size: 1835 2193 178 1358 88 124 3489
Fragments arranged by size:
3489 2193 1835 1358 178 124 88
BsrBI CCG'CTC
Cuts at: 1394 1823 6343 7152 1394
Size: 429 4520 809 3507
Fragments arranged by size:
4520 3507 809 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2755 3964 4233 7760 7934 65
Size: 1183 1507 1209 269 3527 174 1396
Fragments arranged by size:
3527 1507 1396 1209 1183 269 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3037 3895 7909 8096 1700
Size: 110 1227 858 4014 187 2869
Fragments arranged by size:
4014 2869 1227 858 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9265
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9265
BssSI C'ACGA_G
Cuts at: 7325 8337 7325
Size: 1012 8253
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2671 3341 794
Size: 1877 670 6718
Fragments arranged by size:
6718 1877 670
BstBI TT'CG_AA
Cuts at: 2007 8069 2007
Size: 6062 3203
BstEII G'GTnAC_C
Cuts at: 1184 6434 1184
Size: 5250 4015
BstXI CCAn_nnnn'nTGG
Cuts at: 2690 4405 5776 2690
Size: 1715 1371 6179
Fragments arranged by size:
6179 1715 1371
BstYI r'GATC_y
Cuts at: 1972 2059 3804 6595 6962 7347 7364 8816 8902
Size: 87 1745 2791 367 385 17 1452 86
Cuts at: 8902 8914 9260 1972
Size: 12 346 1977
Fragments arranged by size:
2791 1977 1745 1452 385 367 346 87
86 17 12
BstZ17I GTA'TAC
Cuts at: 5761 5761
Size: 9265
Bsu36I CC'TnA_GG
Cuts at: 3731 3731
Size: 9265
BtgI C'CryG_G
Cuts at: 513 1065 2556 513
Size: 552 1491 7222
Fragments arranged by size:
7222 1491 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2106 487
Size: 683 56 880 7646
Fragments arranged by size:
7646 880 683 56
BtsI GCAGTG_nn'
Cuts at: 2701 2773 3856 4281 6781 7575 7595 2701
Size: 72 1083 425 2500 794 20 4371
Fragments arranged by size:
4371 2500 1083 794 425 72 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9265
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2197 2232 2737 2772 529
Size: 35 1633 35 505 35 7022
Fragments arranged by size:
7022 1633 505 35 35 35
DraI TTT'AAA
Cuts at: 4584 5722 6569 4584
Size: 1138 847 7280
Fragments arranged by size:
7280 1138 847
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8272 809
Size: 507 6956 1802
Fragments arranged by size:
6956 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4650 5263 5307
Size: 54 1425 50 275 18 2820 613 44
Cuts at: 5307 5472 6388 6712 7601 9230 8
Size: 165 916 324 889 1629 43
Fragments arranged by size:
2820 1629 1425 916 889 613 324 275
165 54 50 44 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6712 1537
Size: 275 4900 4090
Fragments arranged by size:
4900 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2871 3323 3670 6198 6547 7195 1353
Size: 1518 452 347 2528 349 648 3423
Fragments arranged by size:
3423 2528 1518 648 452 349 347
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7852 8226 8372 387
Size: 1064 138 6263 374 146 1280
Fragments arranged by size:
6263 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5419 5419
Size: 9265
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2215 2472 2660 3320 3541 3654 3797
Size: 393 583 257 188 660 221 113 143
Cuts at: 3797 5585 6144 6263 7328 7925 8712 8980 1239
Size: 1788 559 119 1065 597 787 268 1524
Fragments arranged by size:
1788 1524 1065 787 660 597 583 559
393 268 257 221 188 143 119 113
EcoNI CCTnn'n_nnAGG
Cuts at: 2675 4713 6468 2675
Size: 2038 1755 5472
Fragments arranged by size:
5472 2038 1755
EcoO109I rG'GnC_Cy
Cuts at: 2707 3123 3262 3927 4015 2707
Size: 416 139 665 88 7957
Fragments arranged by size:
7957 665 416 139 88
EcoRI G'AATT_C
Cuts at: 2308 2470 3997 6574 6952 2308
Size: 162 1527 2577 378 4621
Fragments arranged by size:
4621 2577 1527 378 162
EcoRV GAT'ATC
Cuts at: 3490 3490
Size: 9265
FspI TGC'GCA
Cuts at: 7771 7771
Size: 9265
HaeII r_GCGC'y
Cuts at: 1678 2449 2480 2625 2957 4725 8412 1678
Size: 771 31 145 332 1768 3687 2531
Fragments arranged by size:
3687 2531 1768 771 332 145 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3539 3571 3791 3823 4064
Size: 32 135 32 1967 32 220 32 241
Cuts at: 4064 4096 4401 4433 4868 4900 5282 5314 5696
Size: 32 305 32 435 32 382 32 382
Cuts at: 5696 5728 5918 5950 6000 6022 6032 6054 6362
Size: 32 190 32 50 22 10 22 308
Cuts at: 6362 6394 7906 7938 7980 8012 1373
Size: 32 1512 32 42 32 2626
Fragments arranged by size:
2626 1967 1512 435 382 382 308 305
241 220 190 135 50 42 32 32
32 32 32 32 32 32 32 32
32 32 32 22 22 10
HincII GTy'rAC
Cuts at: 669 1657 1938 2932 4788 5886 6238 6609 6855
Size: 988 281 994 1856 1098 352 371 246
Cuts at: 6855 669
Size: 3079
Fragments arranged by size:
3079 1856 1098 994 988 371 352 281
246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9265
HpaI GTT'AAC
Cuts at: 5886 6855 5886
Size: 969 8296
KasI G'GCGC_C
Cuts at: 2445 2621 2445
Size: 176 9089
KpnI G_GTAC'C
Cuts at: 3739 6590 3739
Size: 2851 6414
MfeI C'AATT_G
Cuts at: 6864 6864
Size: 9265
MluI A'CGCG_T
Cuts at: 9141 9141
Size: 9265
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2092 3073 4077 4608 4866 8379 8563 1305
Size: 787 981 1004 531 258 3513 184 2007
Fragments arranged by size:
3513 2007 1004 981 787 531 258 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4652 5265 5309 10
Size: 54 1425 343 2820 613 44 3966
Fragments arranged by size:
3966 2820 1425 613 343 54 44
MslI CAynn'nnrTG
Cuts at: 518 1166 2027 2688 2727 3844 4403 4421 4748
Size: 648 861 661 39 1117 559 18 327
Cuts at: 4748 5019 5754 7223 7582 7741 518
Size: 271 735 1469 359 159 2042
Fragments arranged by size:
2042 1469 1117 861 735 661 648 559
359 327 271 159 39 18
MspA1I CmG'CkG
Cuts at: 1606 2499 2508 2558 2807 3453 4814 5078 6338
Size: 893 9 50 249 646 1361 264 1260
Cuts at: 6338 6394 6681 7358 8506 8751 1606
Size: 56 287 677 1148 245 2120
Fragments arranged by size:
2120 1361 1260 1148 893 677 646 287
264 249 245 56 50 9
NarI GG'CG_CC
Cuts at: 2446 2622 2446
Size: 176 9089
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 8713
NdeI CA'TA_TG
Cuts at: 387 5275 387
Size: 4888 4377
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9265
NotI GC'GGCC_GC
Cuts at: 6712 6712
Size: 9265
NsiI A_TGCA'T
Cuts at: 5275 5275
Size: 9265
NspI r_CATG'y
Cuts at: 1132 1229 3087 4784 5050 6065 6290 6623 8168
Size: 97 1858 1697 266 1015 225 333 1545
Cuts at: 8168 1132
Size: 2229
Fragments arranged by size:
2229 1858 1697 1545 1015 333 266 225
97
PasI CC'CwG_GG
Cuts at: 1348 2302 5054 1348
Size: 954 2752 5559
Fragments arranged by size:
5559 2752 954
PciI A'CATG_T
Cuts at: 3083 8164 3083
Size: 5081 4184
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5362 1108
Size: 4254 5011
PfoI T'CCnGG_A
Cuts at: 2651 6160 6541 2651
Size: 3509 381 5375
Fragments arranged by size:
5375 3509 381
PmeI GTTT'AAAC
Cuts at: 6569 6569
Size: 9265
PmlI CAC'GTG
Cuts at: 3207 3207
Size: 9265
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3545 3577 7334 7366 8847 8879 3545
Size: 32 3757 32 1481 32 3931
Fragments arranged by size:
3931 3757 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3927 3927
Size: 9265
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9265
PsiI TTA'TAA
Cuts at: 6835 6835
Size: 9265
PspOMI G'GGCC_C
Cuts at: 3122 3261 3122
Size: 139 9126
PspXI vC'TCGA_Gb
Cuts at: 4726 4726
Size: 9265
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3206 3238 3206
Size: 32 9233
PstI C_TGCA'G
Cuts at: 830 2047 2812 3396 5077 6617 830
Size: 1217 765 584 1681 1540 3478
Fragments arranged by size:
3478 1681 1540 1217 765 584
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9265
PvuII CAG'CTG
Cuts at: 4814 5078 6338 4814
Size: 264 1260 7741
Fragments arranged by size:
7741 1260 264
SacI G_AGCT'C
Cuts at: 5421 5421
Size: 9265
SacII CC_GC'GG
Cuts at: 2559 2559
Size: 9265
SalI G'TCGA_C
Cuts at: 1936 6607 1936
Size: 4671 4594
SanDI GG'GwC_CC
Cuts at: 3927 3927
Size: 9265
SapI GCTCTTCn'nnn_
Cuts at: 3670 3670
Size: 9265
SbfI CC_TGCA'GG
Cuts at: 6617 6617
Size: 9265
ScaI AGT'ACT
Cuts at: 5345 6056 7513 9225 5345
Size: 711 1457 1712 5385
Fragments arranged by size:
5385 1712 1457 711
SfcI C'TryA_G
Cuts at: 826 1045 2043 2808 3392 4968 5073 5323 5658
Size: 219 998 765 584 1576 105 250 335
Cuts at: 5658 6333 6613 7748 8429 8620 826
Size: 675 280 1135 681 191 1471
Fragments arranged by size:
1576 1471 1135 998 765 681 675 584
335 280 250 219 191 105
SfoI GGC'GCC
Cuts at: 2447 2623 2447
Size: 176 9089
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9265
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9265
SmaI CCC'GGG
Cuts at: 1872 3357 6450 6592 1872
Size: 1485 3093 142 4545
Fragments arranged by size:
4545 3093 1485 142
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3624 4726 5713 7369 8270 8532
Size: 197 926 1681 1102 987 1656 901 262
Cuts at: 8532 820
Size: 1553
Fragments arranged by size:
1681 1656 1553 1102 987 926 901 262
197
SnaBI TAC'GTA
Cuts at: 493 6562 7056 493
Size: 6069 494 2702
Fragments arranged by size:
6069 2702 494
SpeI A'CTAG_T
Cuts at: 152 2192 152
Size: 2040 7225
SphI G_CATG'C
Cuts at: 1132 6065 6290 6623 1132
Size: 4933 225 333 3774
Fragments arranged by size:
4933 3774 333 225
SspI AAT'ATT
Cuts at: 5 52 3028 7189 5
Size: 47 2976 4161 2081
Fragments arranged by size:
4161 2976 2081 47
StuI AGG'CCT
Cuts at: 1517 2291 1517
Size: 774 8491
StyI C'CwwG_G
Cuts at: 513 1065 2208 2440 3655 4548 6704 513
Size: 552 1143 232 1215 893 2156 3074
Fragments arranged by size:
3074 2156 1215 1143 893 552 232
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3062 3708 6040 6434 7292 7309 7462
Size: 1255 1482 646 2332 394 858 17 153
Cuts at: 7462 7647 8092 325
Size: 185 445 1498
Fragments arranged by size:
2332 1498 1482 1255 858 646 445 394
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3711 5343 6054
Size: 274 80 33 51 1443 1734 1632 711
Cuts at: 6054 7511 9223 96
Size: 1457 1712 138
Fragments arranged by size:
1734 1712 1632 1457 1443 711 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2040 2072 5994 6026 9225 9257 2040
Size: 32 3922 32 3199 32 2048
Fragments arranged by size:
3922 3199 2048 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9265
XbaI T'CTAG_A
Cuts at: 6601 6601
Size: 9265
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2447 2693 3148 5273 6422 2447
Size: 246 455 2125 1149 5290
Fragments arranged by size:
5290 2125 1149 455 246
XhoI C'TCGA_G
Cuts at: 1943 4726 5713 1943
Size: 2783 987 5495
Fragments arranged by size:
5495 2783 987
XmaI C'CCGG_G
Cuts at: 1870 3355 6448 6590 1870
Size: 1485 3093 142 4545
Fragments arranged by size:
4545 3093 1485 142
XmnI GAAnn'nnTTC
Cuts at: 7394 7394
Size: 9265
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7073 276
Size: 53 83 186 936 5539 2468
Fragments arranged by size:
5539 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII DraIII FalI FseI FspAI NaeI
NgoMIV NruI PacI RsrII SexAI SfiI SrfI SwaI