Restriction map for
pFN21ASDA1277
(Circular) (Six-base) MAPSORT of: pFN21ASDA1277.seq Check: 4002 from: 1 to: 7756
pFN21ASDA1277 7756 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4781 5566 278
Size: 53 83 186 936 3245 785 2468
Fragments arranged by size:
3245 2468 936 785 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4443 5099 1937
Size: 2506 656 4594
Fragments arranged by size:
4594 2506 656
Acc65I G'GTAC_C
Cuts at: 3666 5077 3666
Size: 1411 6345
AclI AA'CG_TT
Cuts at: 1563 2837 5883 6256 1563
Size: 1274 3046 373 3063
Fragments arranged by size:
3063 3046 1274 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2522 2872 3671 3818 3866 4027 4029 4589
Size: 890 350 799 147 48 161 2 560
Cuts at: 4589 4743 5030 5819 7203 7471 1632
Size: 154 287 789 1384 268 1917
Fragments arranged by size:
1917 1384 890 799 789 560 350 287
268 161 154 147 48 2
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7559
AflIII A'CryG_T
Cuts at: 1203 2769 6655 7632 1203
Size: 1566 3886 977 1327
Fragments arranged by size:
3886 1566 1327 977
AgeI A'CCGG_T
Cuts at: 6587 6587
Size: 7756
AhdI GACnn_n'nnGTC
Cuts at: 4252 6485 4252
Size: 2233 5523
AleI CACnn'nnGTG
Cuts at: 1166 4992 1166
Size: 3826 3930
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7756
AlwNI CAG_nnn'CTG
Cuts at: 3628 4196 5499 7071 3628
Size: 568 1303 1572 4313
Fragments arranged by size:
4313 1572 1303 568
ApaI G_GGCC'C
Cuts at: 4671 4671
Size: 7756
ApaLI G'TGCA_C
Cuts at: 5813 6969 5813
Size: 1156 6600
ApoI r'AATT_y
Cuts at: 1493 3220 3301 5033 5065 5291 5443 7552 1493
Size: 1727 81 1732 32 226 152 2109 1697
Fragments arranged by size:
2109 1732 1727 1697 226 152 81 32
AseI AT'TA_AT
Cuts at: 160 1032 6310 160
Size: 872 5278 1606
Fragments arranged by size:
5278 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7756
AvaI C'yCGr_G
Cuts at: 1870 1943 2519 3681 4235 5081 1870
Size: 73 576 1162 554 846 4545
Fragments arranged by size:
4545 1162 846 576 554 73
BamHI G'GATC_C
Cuts at: 2428 2554 5086 5453 2428
Size: 126 2532 367 4731
Fragments arranged by size:
4731 2532 367 126
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2197 3658 3666 5077 6532
Size: 325 217 627 410 1461 8 1411 1455
Cuts at: 6532 618
Size: 1842
Fragments arranged by size:
1842 1461 1455 1411 627 410 325 217
8
BanII G_rGCy'C
Cuts at: 1391 2703 2783 4671 4954 5075 1391
Size: 1312 80 1888 283 121 4072
Fragments arranged by size:
4072 1888 1312 283 121 80
BbeI G_GCGC'C
Cuts at: 3662 3662
Size: 7756
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2983 3042 3141 3979 4858 928
Size: 971 1084 59 99 838 879 3826
Fragments arranged by size:
3826 1084 971 879 838 99 59
BbvCI CC'TCA_GC
Cuts at: 3834 4271 3834
Size: 437 7319
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3644 3678 3940 3974 5947 5981 1903
Size: 34 1707 34 262 34 1973 34 3678
Fragments arranged by size:
3678 1973 1707 262 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5648 6858 5648
Size: 1210 6546
BclI T'GATC_A
Cuts at: 1548 2046 1548
Size: 498 7258
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2078 6367 136
Size: 107 122 71 631 605 406 4289 1525
Fragments arranged by size:
4289 1525 631 605 406 122 107 71
BglII A'GATC_T
Cuts at: 7751 7751
Size: 7756
BlpI GC'TnA_GC
Cuts at: 4346 5173 4346
Size: 827 6929
Bme1580I G_kGCm'C
Cuts at: 1790 2202 4671 5817 6973 1790
Size: 412 2469 1146 1156 2573
Fragments arranged by size:
2573 2469 1156 1146 412
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7756
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2554 3369 3529
Size: 416 53 83 399 24 1137 815 160
Cuts at: 3529 3853 4971 5487 6445 442
Size: 324 1118 516 958 1753
Fragments arranged by size:
1753 1137 1118 958 815 516 416 399
324 160 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7756
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3568 3568
Size: 7756
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3957 4005 4334 4471 4880 6416 1239
Size: 2718 48 329 137 409 1536 2579
Fragments arranged by size:
2718 2579 1536 409 329 137 48
Bpu10I CC'TnA_GC
Cuts at: 2405 2709 3834 4271 4505 2405
Size: 304 1125 437 234 5656
Fragments arranged by size:
5656 1125 437 304 234
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2783 5881 6746 7044 2783
Size: 3098 865 298 3495
Fragments arranged by size:
3495 3098 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3423 6419 882
Size: 617 1924 2996 2219
Fragments arranged by size:
2996 2219 1924 617
BsaAI yAC'GTr
Cuts at: 493 1204 3769 4243 5053 5547 493
Size: 711 2565 474 810 494 2702
Fragments arranged by size:
2702 2565 810 711 494 474
BsaBI GATnn'nnATC
Cuts at: 7750 7750
Size: 7756
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3659 4463 4778 5563
Size: 53 83 186 936 2126 804 315 785
Cuts at: 5563 5945 6580 275
Size: 382 635 1451
Fragments arranged by size:
2126 1451 936 804 785 635 382 315
186 83 53
BsaWI w'CCGG_w
Cuts at: 2754 3501 6189 6587 6861 7008 7419 2754
Size: 747 2688 398 274 147 411 3091
Fragments arranged by size:
3091 2688 747 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2260 2290 1178
Size: 30 165 30 857 30 6644
Fragments arranged by size:
6644 857 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2281 2284 2904 2938 3134 3325 3614 4255
Size: 1095 3 620 34 196 191 289 641
Cuts at: 4255 4333 4808 4825 1186
Size: 78 475 17 4117
Fragments arranged by size:
4117 1095 641 620 475 289 196 191
78 34 17 3
BseYI C'CCAG_C
Cuts at: 2059 2081 2213 2436 3094 3395 4144 6959 7455
Size: 22 132 223 658 301 749 2815 496
Cuts at: 7455 2059
Size: 2360
Fragments arranged by size:
2815 2360 749 658 496 301 223 132
22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2193 3027 1163
Size: 573 457 834 5892
Fragments arranged by size:
5892 834 573 457
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5206 5967 6995 1540
Size: 275 186 3205 761 1028 2301
Fragments arranged by size:
3205 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2339 4242 4457 4989 5817 5902 6973 2339
Size: 1903 215 532 828 85 1071 3122
Fragments arranged by size:
3122 1903 1071 828 532 215 85
BsmI GAATG_Cn'
Cuts at: 5267 5360 5267
Size: 93 7663
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2202 2339 2703 2783 4242 4457 4671
Size: 399 412 137 364 80 1459 215 214
Cuts at: 4671 4954 4989 5075 5817 5902 6973 1391
Size: 283 35 86 742 85 1071 2174
Fragments arranged by size:
2174 1459 1071 742 412 399 364 283
215 214 137 86 85 80 35
BspEI T'CCGG_A
Cuts at: 3501 7419 3501
Size: 3918 3838
BspHI T'CATG_A
Cuts at: 3822 4116 5645 7375 3822
Size: 294 1529 1730 4203
Fragments arranged by size:
4203 1730 1529 294
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2497 3292 5111 844
Size: 1653 795 1819 3489
Fragments arranged by size:
3489 1819 1653 795
BsrBI CCG'CTC
Cuts at: 1394 1823 4906 5643 1394
Size: 429 3083 737 3507
Fragments arranged by size:
3507 3083 737 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4332 6251 6425 65
Size: 1183 3084 1919 174 1396
Fragments arranged by size:
3084 1919 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4554 4917 6400 6587 1700
Size: 110 2744 363 1483 187 2869
Fragments arranged by size:
2869 2744 1483 363 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7756
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7756
BssSI C'ACGA_G
Cuts at: 5816 6828 5816
Size: 1012 6744
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7756
BstBI TT'CG_AA
Cuts at: 2007 6560 2007
Size: 4553 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7756
BstYI r'GATC_y
Cuts at: 1972 2428 2554 3145 4059 5086 5453 5838 5855
Size: 456 126 591 914 1027 367 385 17
Cuts at: 5855 7307 7393 7405 7751 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1027 914 591 456 385 367
346 126 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 4437 513
Size: 552 957 2415 3832
Fragments arranged by size:
3832 2415 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4388 487
Size: 683 56 3162 3855
Fragments arranged by size:
3855 3162 683 56
BtsI GCAGTG_nn'
Cuts at: 2370 2587 4499 5272 6066 6086 2370
Size: 217 1912 773 794 20 4040
Fragments arranged by size:
4040 1912 794 773 217 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7756
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7721
DraI TTT'AAA
Cuts at: 5060 5060
Size: 7756
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6763 809
Size: 507 5447 1802
Fragments arranged by size:
5447 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5203 6092 7721
Size: 54 1425 50 275 18 3373 889 1629
Cuts at: 7721 8
Size: 43
Fragments arranged by size:
3373 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5203 1537
Size: 275 3391 4090
Fragments arranged by size:
4090 3391 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3247 3446 3518 3719 4265 4518 5686 1353
Size: 1894 199 72 201 546 253 1168 3423
Fragments arranged by size:
3423 1894 1168 546 253 201 199 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3138 4268 4838 6343 6717 6863
Size: 1064 138 1549 1130 570 1505 374 146
Cuts at: 6863 387
Size: 1280
Fragments arranged by size:
1549 1505 1280 1130 1064 570 374 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2522 2872 3671 3818 3866 3957 4005
Size: 393 890 350 799 147 48 91 48
Cuts at: 4005 4027 4029 4334 4471 4589 4743 4880 5030
Size: 22 2 305 137 118 154 137 150
Cuts at: 5030 5819 6416 7203 7471 1239
Size: 789 597 787 268 1524
Fragments arranged by size:
1524 890 799 789 787 597 393 350
305 268 154 150 147 137 137 118
91 48 48 22 2
EcoO109I rG'GnC_Cy
Cuts at: 2383 2745 3363 4528 4667 4668 2383
Size: 362 618 1165 139 1 5471
Fragments arranged by size:
5471 1165 618 362 139 1
EcoRI G'AATT_C
Cuts at: 5065 5443 5065
Size: 378 7378
EcoRV GAT'ATC
Cuts at: 2172 2172
Size: 7756
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2491 3232 4547 2491
Size: 741 1315 5700
Fragments arranged by size:
5700 1315 741
FspI TGC'GCA
Cuts at: 6262 6262
Size: 7756
HaeII r_GCGC'y
Cuts at: 1678 3628 3662 4003 4385 6903 1678
Size: 1950 34 341 382 2518 2531
Fragments arranged by size:
2531 2518 1950 382 341 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2156 2188 2477 2509 2600
Size: 32 135 32 584 32 289 32 91
Cuts at: 2600 2632 4074 4106 4238 4270 4568 4600 4703
Size: 32 1442 32 132 32 298 32 103
Cuts at: 4703 4735 6397 6429 6471 6503 1373
Size: 32 1662 32 42 32 2626
Fragments arranged by size:
2626 1662 1442 584 298 289 135 132
103 91 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3634 4444 4540 4549 5100 5346
Size: 988 281 1696 810 96 9 551 246
Cuts at: 5346 669
Size: 3079
Fragments arranged by size:
3079 1696 988 810 551 281 246 96
9
HindIII A'AGCT_T
Cuts at: 748 4601 748
Size: 3853 3903
HpaI GTT'AAC
Cuts at: 5346 5346
Size: 7756
KasI G'GCGC_C
Cuts at: 3658 3658
Size: 7756
KpnI G_GTAC'C
Cuts at: 3670 5081 3670
Size: 1411 6345
MfeI C'AATT_G
Cuts at: 5355 5355
Size: 7756
MluI A'CGCG_T
Cuts at: 7632 7632
Size: 7756
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3319 3783 6870 7054 1305
Size: 2014 464 3087 184 2007
Fragments arranged by size:
3087 2014 2007 464 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5934
Fragments arranged by size:
5934 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2027 4421 4992 5714 6073 6232 518
Size: 648 861 2394 571 722 359 159 2042
Fragments arranged by size:
2394 2042 861 722 648 571 359 159
MspA1I CmG'CkG
Cuts at: 1606 2063 4222 4519 4810 4992 5172 5849 6997
Size: 457 2159 297 291 182 180 677 1148
Cuts at: 6997 7242 1606
Size: 245 2120
Fragments arranged by size:
2159 2120 1148 677 457 297 291 245
182 180
NaeI GCC'GGC
Cuts at: 4919 4919
Size: 7756
NarI GG'CG_CC
Cuts at: 3659 3659
Size: 7756
NcoI C'CATG_G
Cuts at: 513 1065 2022 4437 513
Size: 552 957 2415 3832
Fragments arranged by size:
3832 2415 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7756
NgoMIV G'CCGG_C
Cuts at: 4917 4917
Size: 7756
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7756
NotI GC'GGCC_GC
Cuts at: 5203 5203
Size: 7756
NspI r_CATG'y
Cuts at: 1132 1229 2849 5114 6659 1132
Size: 97 1620 2265 1545 2229
Fragments arranged by size:
2265 2229 1620 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2748 4150 1348
Size: 1400 1402 4954
Fragments arranged by size:
4954 1402 1400
PciI A'CATG_T
Cuts at: 6655 6655
Size: 7756
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2462 3541 3628 3885 4375 4858 1108
Size: 1354 1079 87 257 490 483 4006
Fragments arranged by size:
4006 1354 1079 490 483 257 87
PfoI T'CCnGG_A
Cuts at: 4614 4857 4899 4614
Size: 243 42 7471
Fragments arranged by size:
7471 243 42
PmeI GTTT'AAAC
Cuts at: 5060 5060
Size: 7756
PmlI CAC'GTG
Cuts at: 4243 4243
Size: 7756
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5825 5857 7338 7370 5825
Size: 32 1481 32 6211
Fragments arranged by size:
6211 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2383 3363 4528 2383
Size: 980 1165 5611
Fragments arranged by size:
5611 1165 980
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7756
PsiI TTA'TAA
Cuts at: 5326 5326
Size: 7756
PspOMI G'GGCC_C
Cuts at: 4667 4667
Size: 7756
PspXI vC'TCGA_Gb
Cuts at: 2519 4235 2519
Size: 1716 6040
PstI C_TGCA'G
Cuts at: 830 2068 3048 4809 5108 830
Size: 1238 980 1761 299 3478
Fragments arranged by size:
3478 1761 1238 980 299
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7756
PvuII CAG'CTG
Cuts at: 2063 2063
Size: 7756
SalI G'TCGA_C
Cuts at: 1936 4442 5098 1936
Size: 2506 656 4594
Fragments arranged by size:
4594 2506 656
SanDI GG'GwC_CC
Cuts at: 3363 3363
Size: 7756
SapI GCTCTTCn'nnn_
Cuts at: 3719 3719
Size: 7756
SbfI CC_TGCA'GG
Cuts at: 5108 5108
Size: 7756
ScaI AGT'ACT
Cuts at: 3722 6004 7716 3722
Size: 2282 1712 3762
Fragments arranged by size:
3762 2282 1712
SexAI A'CCwGG_T
Cuts at: 4284 4284
Size: 7756
SfcI C'TryA_G
Cuts at: 826 1045 2064 3044 4805 5104 6239 6920 7111
Size: 219 1019 980 1761 299 1135 681 191
Cuts at: 7111 826
Size: 1471
Fragments arranged by size:
1761 1471 1135 1019 980 681 299 219
191
SfoI GGC'GCC
Cuts at: 3660 3660
Size: 7756
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7756
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7756
SmaI CCC'GGG
Cuts at: 1872 3683 5083 1872
Size: 1811 1400 4545
Fragments arranged by size:
4545 1811 1400
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2519 2762 4235 5860 6761 7023
Size: 197 926 576 243 1473 1625 901 262
Cuts at: 7023 820
Size: 1553
Fragments arranged by size:
1625 1553 1473 926 901 576 262 243
197
SnaBI TAC'GTA
Cuts at: 493 5053 5547 493
Size: 4560 494 2702
Fragments arranged by size:
4560 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7756
SphI G_CATG'C
Cuts at: 1132 5114 1132
Size: 3982 3774
SspI AAT'ATT
Cuts at: 5 52 5680 5
Size: 47 5628 2081
Fragments arranged by size:
5628 2081 47
StuI AGG'CCT
Cuts at: 1517 4817 1517
Size: 3300 4456
StyI C'CwwG_G
Cuts at: 513 1065 2022 3424 3879 4437 5195 513
Size: 552 957 1402 455 558 758 3074
Fragments arranged by size:
3074 1402 957 758 558 552 455
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2227 3797 5783 5800 5953 6138 6583
Size: 1255 647 1570 1986 17 153 185 445
Cuts at: 6583 325
Size: 1498
Fragments arranged by size:
1986 1570 1498 1255 647 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3720 6002 7714
Size: 274 80 33 51 1443 1743 2282 1712
Cuts at: 7714 96
Size: 138
Fragments arranged by size:
2282 1743 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2260 2292 3005 3037 4185 4217 7716 7748 2260
Size: 32 713 32 1148 32 3499 32 2268
Fragments arranged by size:
3499 2268 1148 713 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4544 1316
Size: 3228 4528
XbaI T'CTAG_A
Cuts at: 3142 5092 3142
Size: 1950 5806
XhoI C'TCGA_G
Cuts at: 1943 2519 4235 1943
Size: 576 1716 5464
Fragments arranged by size:
5464 1716 576
XmaI C'CCGG_G
Cuts at: 1870 3681 5081 1870
Size: 1811 1400 4545
Fragments arranged by size:
4545 1811 1400
XmnI GAAnn'nnTTC
Cuts at: 3138 4623 5885 3138
Size: 1485 1262 5009
Fragments arranged by size:
5009 1485 1262
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4779 5564 276
Size: 53 83 186 936 3245 785 2468
Fragments arranged by size:
3245 2468 936 785 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI
FspAI NruI NsiI PacI PsrI RsrII SacI SacII
SfiI SrfI SwaI XcmI