Restriction map for
pFN21ASDA1269
(Circular) (Six-base) MAPSORT of: pFN21ASDA1269.seq Check: 5003 from: 1 to: 6319
pFN21ASDA1269 6319 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2552 3133 2552
Size: 581 5738
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4129 278
Size: 53 83 186 936 2593 2468
Fragments arranged by size:
2593 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3662 1937
Size: 1725 4594
Acc65I G'GTAC_C
Cuts at: 3640 3640
Size: 6319
AclI AA'CG_TT
Cuts at: 1563 4446 4819 1563
Size: 2883 373 3063
Fragments arranged by size:
3063 2883 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2283 2459 2762 3041 3251 4382 5766 6034
Size: 651 176 303 279 210 1131 1384 268
Cuts at: 6034 1632
Size: 1917
Fragments arranged by size:
1917 1384 1131 651 303 279 268 210
176
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6122
AflIII A'CryG_T
Cuts at: 1203 3372 5218 6195 1203
Size: 2169 1846 977 1327
Fragments arranged by size:
2169 1846 1327 977
AgeI A'CCGG_T
Cuts at: 5150 5150
Size: 6319
AhdI GACnn_n'nnGTC
Cuts at: 5048 5048
Size: 6319
AleI CACnn'nnGTG
Cuts at: 1166 2087 3521 1166
Size: 921 1434 3964
Fragments arranged by size:
3964 1434 921
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6319
AlwNI CAG_nnn'CTG
Cuts at: 2791 3542 4062 5634 2791
Size: 751 520 1572 3476
Fragments arranged by size:
3476 1572 751 520
ApaI G_GGCC'C
Cuts at: 3476 3476
Size: 6319
ApaLI G'TGCA_C
Cuts at: 2045 2414 4376 5532 2045
Size: 369 1962 1156 2832
Fragments arranged by size:
2832 1962 1156 369
ApoI r'AATT_y
Cuts at: 1493 2408 3628 3854 4006 6115 1493
Size: 915 1220 226 152 2109 1697
Fragments arranged by size:
2109 1697 1220 915 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4873 160
Size: 872 3841 1606
Fragments arranged by size:
3841 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6319
AvaI C'yCGr_G
Cuts at: 1870 1943 2961 3178 3305 3548 3644 1870
Size: 73 1018 217 127 243 96 4545
Fragments arranged by size:
4545 1018 243 217 127 96 73
BamHI G'GATC_C
Cuts at: 3649 4016 3649
Size: 367 5952
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3271 3640 5095 618
Size: 325 217 627 1484 369 1455 1842
Fragments arranged by size:
1842 1484 1455 627 369 325 217
BanII G_rGCy'C
Cuts at: 1391 2578 3476 3638 1391
Size: 1187 898 162 4072
Fragments arranged by size:
4072 1187 898 162
BbeI G_GCGC'C
Cuts at: 3275 3275
Size: 6319
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2123 2688 928
Size: 971 224 565 4559
Fragments arranged by size:
4559 971 565 224
BbvCI CC'TCA_GC
Cuts at: 3041 3041
Size: 6319
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4510 4544 1903
Size: 34 2573 34 3678
Fragments arranged by size:
3678 2573 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3305 4211 5421 3305
Size: 906 1210 4203
Fragments arranged by size:
4203 1210 906
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6319
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3134 3167 4930
Size: 107 122 71 631 605 1462 33 1763
Cuts at: 4930 136
Size: 1525
Fragments arranged by size:
1763 1525 1462 631 605 122 107 71
33
BglII A'GATC_T
Cuts at: 6314 6314
Size: 6319
BlpI GC'TnA_GC
Cuts at: 3736 3736
Size: 6319
Bme1580I G_kGCm'C
Cuts at: 1790 2049 2418 3189 3213 3476 3534 4380 5536
Size: 259 369 771 24 263 58 846 1156
Cuts at: 5536 1790
Size: 2573
Fragments arranged by size:
2573 1156 846 771 369 263 259 58
24
BmgBI CAC'GTC
Cuts at: 1318 2038 2601 2641 1318
Size: 720 563 40 4996
Fragments arranged by size:
4996 720 563 40
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3148 3158 4050
Size: 416 53 83 399 24 1731 10 892
Cuts at: 4050 5008 442
Size: 958 1753
Fragments arranged by size:
1753 1731 958 892 416 399 83 53
24 10
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6319
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2345 2345
Size: 6319
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2356 2999 3496 4979 1239
Size: 1117 643 497 1483 2579
Fragments arranged by size:
2579 1483 1117 643 497
Bpu10I CC'TnA_GC
Cuts at: 2480 2588 3041 3392 2480
Size: 108 453 351 5407
Fragments arranged by size:
5407 453 351 108
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2219 4444 5309 5607 2219
Size: 2225 865 298 2931
Fragments arranged by size:
2931 2225 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2655 2666 2792 3076 4982 882
Size: 617 1156 11 126 284 1906 2219
Fragments arranged by size:
2219 1906 1156 617 284 126 11
BsaAI yAC'GTr
Cuts at: 493 1204 3110 3616 4110 493
Size: 711 1906 506 494 2702
Fragments arranged by size:
2702 1906 711 506 494
BsaBI GATnn'nnATC
Cuts at: 2997 6313 2997
Size: 3316 3003
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2748 3065 3272 4126
Size: 53 83 186 936 1215 317 207 854
Cuts at: 4126 4508 5143 275
Size: 382 635 1451
Fragments arranged by size:
1451 1215 936 854 635 382 317 207
186 83 53
BsaWI w'CCGG_w
Cuts at: 2077 4752 5150 5424 5571 5982 2077
Size: 2675 398 274 147 411 2414
Fragments arranged by size:
2675 2414 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2565 2595 2972 3002 1178
Size: 30 165 30 1162 30 377 30 4495
Fragments arranged by size:
4495 1162 377 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2069 2215 3572 1186
Size: 883 146 1357 3933
Fragments arranged by size:
3933 1357 883 146
BseYI C'CCAG_C
Cuts at: 2229 3033 3195 3347 5522 6018 2229
Size: 804 162 152 2175 496 2530
Fragments arranged by size:
2530 2175 804 496 162 152
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2897 3428 1163
Size: 573 1161 531 4054
Fragments arranged by size:
4054 1161 573 531
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2607 3129 3402 3769 4530 5558
Size: 275 186 606 522 273 367 761 1028
Cuts at: 5558 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 606 522 367 275 273
186
BsiHKAI G_wGCw'C
Cuts at: 2049 2418 3054 3150 3398 3564 4380 4465 5536
Size: 369 636 96 248 166 816 85 1071
Cuts at: 5536 2049
Size: 2832
Fragments arranged by size:
2832 1071 816 636 369 248 166 96
85
BsmI GAATG_Cn'
Cuts at: 3830 3923 3830
Size: 93 6226
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2049 2418 2578 3054 3150 3189 3213
Size: 399 259 369 160 476 96 39 24
Cuts at: 3213 3398 3476 3534 3564 3638 4380 4465 5536
Size: 185 78 58 30 74 742 85 1071
Cuts at: 5536 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 476 399 369 259 185
160 96 85 78 74 58 39 30
24
BspEI T'CCGG_A
Cuts at: 2077 5982 2077
Size: 3905 2414
BspHI T'CATG_A
Cuts at: 3054 4208 5938 3054
Size: 1154 1730 3435
Fragments arranged by size:
3435 1730 1154
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2552 3133 3674 844
Size: 1708 581 541 3489
Fragments arranged by size:
3489 1708 581 541
BsrBI CCG'CTC
Cuts at: 1394 1823 2943 3607 4206 1394
Size: 429 1120 664 599 3507
Fragments arranged by size:
3507 1120 664 599 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4814 4988 65
Size: 1183 3566 174 1396
Fragments arranged by size:
3566 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2750 3067 3124 4963 5150 1700
Size: 110 940 317 57 1839 187 2869
Fragments arranged by size:
2869 1839 940 317 187 110 57
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6319
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6319
BssSI C'ACGA_G
Cuts at: 3602 4379 5391 3602
Size: 777 1012 4530
Fragments arranged by size:
4530 1012 777
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3542 794
Size: 2748 3571
BstBI TT'CG_AA
Cuts at: 2007 5123 2007
Size: 3116 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6319
BstXI CCAn_nnnn'nTGG
Cuts at: 3250 3355 3580 3250
Size: 105 225 5989
Fragments arranged by size:
5989 225 105
BstYI r'GATC_y
Cuts at: 1972 3360 3649 4016 4401 4418 5870 5956 5968
Size: 1388 289 367 385 17 1452 86 12
Cuts at: 5968 6314 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1388 385 367 346 289 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3555 3555
Size: 6319
BtgI C'CryG_G
Cuts at: 513 1065 2022 2208 2700 3132 3588 513
Size: 552 957 186 492 432 456 3244
Fragments arranged by size:
3244 957 552 492 456 432 186
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5580
Fragments arranged by size:
5580 683 56
BtsI GCAGTG_nn'
Cuts at: 2082 2094 2104 2993 3835 4629 4649 2082
Size: 12 10 889 842 794 20 3752
Fragments arranged by size:
3752 889 842 794 20 12 10
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6319
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6284
DraI TTT'AAA
Cuts at: 3623 3623
Size: 6319
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5326 809
Size: 507 4010 1802
Fragments arranged by size:
4010 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2421 2604 2703
Size: 54 1425 50 275 18 591 183 99
Cuts at: 2703 2916 3126 3399 3567 3766 4655 6284 8
Size: 213 210 273 168 199 889 1629 43
Fragments arranged by size:
1629 1425 889 591 275 273 213 210
199 183 168 99 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2604 3126 3399 3766 1537
Size: 275 792 522 273 367 4090
Fragments arranged by size:
4090 792 522 367 275 273
EarI CTCTTCn'nnn_
Cuts at: 1353 2259 2444 3334 4249 1353
Size: 906 185 890 915 3423
Fragments arranged by size:
3423 915 906 890 185
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4906 5280 5426 387
Size: 1064 138 3317 374 146 1280
Fragments arranged by size:
3317 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2283 2356 2459 2762 2999 3041 3251
Size: 393 651 73 103 303 237 42 210
Cuts at: 3251 3496 4382 4979 5766 6034 1239
Size: 245 886 597 787 268 1524
Fragments arranged by size:
1524 886 787 651 597 393 303 268
245 237 210 103 73 42
EcoNI CCTnn'n_nnAGG
Cuts at: 3254 3254
Size: 6319
EcoO109I rG'GnC_Cy
Cuts at: 2830 2965 3473 2830
Size: 135 508 5676
Fragments arranged by size:
5676 508 135
EcoRI G'AATT_C
Cuts at: 3628 4006 3628
Size: 378 5941
FspI TGC'GCA
Cuts at: 4825 4825
Size: 6319
HaeII r_GCGC'y
Cuts at: 1678 3173 3275 5466 1678
Size: 1495 102 2191 2531
Fragments arranged by size:
2531 2191 1495 102
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3303 3335 4960 4992 5034
Size: 32 135 32 1731 32 1625 32 42
Cuts at: 5034 5066 1373
Size: 32 2626
Fragments arranged by size:
2626 1731 1625 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3663 3909 669
Size: 988 281 1725 246 3079
Fragments arranged by size:
3079 1725 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6319
HpaI GTT'AAC
Cuts at: 3909 3909
Size: 6319
KasI G'GCGC_C
Cuts at: 3271 3271
Size: 6319
KpnI G_GTAC'C
Cuts at: 3644 3644
Size: 6319
MfeI C'AATT_G
Cuts at: 3519 3918 3519
Size: 399 5920
MluI A'CGCG_T
Cuts at: 3372 6195 3372
Size: 2823 3496
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2562 5433 5617 1305
Size: 1257 2871 184 2007
Fragments arranged by size:
2871 2007 1257 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2918 10
Size: 54 1425 343 1086 3411
Fragments arranged by size:
3411 1425 1086 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2087 2363 2462 3521 3578 4277 4636
Size: 648 921 276 99 1059 57 699 359
Cuts at: 4636 4795 518
Size: 159 2042
Fragments arranged by size:
2042 1059 921 699 648 359 276 159
99 57
MspA1I CmG'CkG
Cuts at: 1606 2195 2204 2210 2702 2821 3199 3301 3445
Size: 589 9 6 492 119 378 102 144
Cuts at: 3445 3451 3735 4412 5560 5805 1606
Size: 6 284 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 589 492 378 284 245
144 119 102 9 6 6
NaeI GCC'GGC
Cuts at: 2752 2752
Size: 6319
NarI GG'CG_CC
Cuts at: 3272 3272
Size: 6319
NcoI C'CATG_G
Cuts at: 513 1065 2022 3132 3588 513
Size: 552 957 1110 456 3244
Fragments arranged by size:
3244 1110 957 552 456
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6319
NgoMIV G'CCGG_C
Cuts at: 2750 2750
Size: 6319
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6319
NotI GC'GGCC_GC
Cuts at: 3766 3766
Size: 6319
NspI r_CATG'y
Cuts at: 1132 1229 2393 2893 3677 5222 1132
Size: 97 1164 500 784 1545 2229
Fragments arranged by size:
2229 1545 1164 784 500 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6319
PciI A'CATG_T
Cuts at: 5218 5218
Size: 6319
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2791 1108
Size: 1683 4636
PfoI T'CCnGG_A
Cuts at: 2772 2826 2772
Size: 54 6265
PmeI GTTT'AAAC
Cuts at: 3623 3623
Size: 6319
PmlI CAC'GTG
Cuts at: 3110 3110
Size: 6319
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4388 4420 5901 5933 4388
Size: 32 1481 32 4774
Fragments arranged by size:
4774 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2830 2965 2830
Size: 135 6184
PshAI GACnn'nnGTC
Cuts at: 1613 3068 1613
Size: 1455 4864
PsiI TTA'TAA
Cuts at: 3889 3889
Size: 6319
PspOMI G'GGCC_C
Cuts at: 3472 3472
Size: 6319
PstI C_TGCA'G
Cuts at: 830 2089 2817 2844 3450 3486 3671 830
Size: 1259 728 27 606 36 185 3478
Fragments arranged by size:
3478 1259 728 606 185 36 27
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6319
PvuII CAG'CTG
Cuts at: 2204 3199 3451 2204
Size: 995 252 5072
Fragments arranged by size:
5072 995 252
SacII CC_GC'GG
Cuts at: 2211 2703 2211
Size: 492 5827
SalI G'TCGA_C
Cuts at: 1936 3661 1936
Size: 1725 4594
SbfI CC_TGCA'GG
Cuts at: 3671 3671
Size: 6319
ScaI AGT'ACT
Cuts at: 4567 6279 4567
Size: 1712 4607
SexAI A'CCwGG_T
Cuts at: 2898 2898
Size: 6319
SfcI C'TryA_G
Cuts at: 826 1045 2085 2813 2840 3446 3482 3667 4802
Size: 219 1040 728 27 606 36 185 1135
Cuts at: 4802 5483 5674 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1040 728 681 606 219 191
185 36 27
SfiI GGCCn_nnn'nGGCC
Cuts at: 3134 3134
Size: 6319
SfoI GGC'GCC
Cuts at: 3273 3273
Size: 6319
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6319
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6319
SmaI CCC'GGG
Cuts at: 1872 2963 3180 3550 3646 1872
Size: 1091 217 370 96 4545
Fragments arranged by size:
4545 1091 370 217 96
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2234 4423 5324 5586 820
Size: 197 926 291 2189 901 262 1553
Fragments arranged by size:
2189 1553 926 901 291 262 197
SnaBI TAC'GTA
Cuts at: 493 3616 4110 493
Size: 3123 494 2702
Fragments arranged by size:
3123 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6319
SphI G_CATG'C
Cuts at: 1132 2893 3677 1132
Size: 1761 784 3774
Fragments arranged by size:
3774 1761 784
SrfI GCCC'GGGC
Cuts at: 3180 3180
Size: 6319
SspI AAT'ATT
Cuts at: 5 52 4243 5
Size: 47 4191 2081
Fragments arranged by size:
4191 2081 47
StuI AGG'CCT
Cuts at: 1517 2811 3500 1517
Size: 1294 689 4336
Fragments arranged by size:
4336 1294 689
StyI C'CwwG_G
Cuts at: 513 1065 2022 3012 3132 3214 3588 3758 513
Size: 552 957 990 120 82 374 170 3074
Fragments arranged by size:
3074 990 957 552 374 170 120 82
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3167 4346 4363 4516 4701 5146 325
Size: 1255 1587 1179 17 153 185 445 1498
Fragments arranged by size:
1587 1498 1255 1179 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2610 2857 4565
Size: 274 80 33 51 1443 633 247 1708
Cuts at: 4565 6277 96
Size: 1712 138
Fragments arranged by size:
1712 1708 1443 633 274 247 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2565 2597 2972 3004 6279 6311 2565
Size: 32 375 32 3275 32 2573
Fragments arranged by size:
3275 2573 375 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2528 2765 1316
Size: 1212 237 4870
Fragments arranged by size:
4870 1212 237
XbaI T'CTAG_A
Cuts at: 3655 3655
Size: 6319
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3503 3577 3503
Size: 74 6245
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6319
XmaI C'CCGG_G
Cuts at: 1870 2961 3178 3548 3644 1870
Size: 1091 217 370 96 4545
Fragments arranged by size:
4545 1091 370 217 96
XmnI GAAnn'nnTTC
Cuts at: 4448 4448
Size: 6319
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4127 276
Size: 53 83 186 936 2593 2468
Fragments arranged by size:
2593 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NruI
NsiI PacI PspXI PsrI RsrII SacI SanDI SapI
SwaI