Restriction map for
pFN21ASDA1260
(Circular) (Six-base) MAPSORT of: pFN21ASDA1260.seq Check: 2607 from: 1 to: 7177
pFN21ASDA1260 7177 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4295 4295
Size: 7177
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3066 3573 4987 278
Size: 53 83 186 936 1530 507 1414 2468
Fragments arranged by size:
2468 1530 1414 936 507 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4470 4520 1937
Size: 2533 50 4594
Fragments arranged by size:
4594 2533 50
Acc65I G'GTAC_C
Cuts at: 4498 4498
Size: 7177
AclI AA'CG_TT
Cuts at: 1563 5304 5677 1563
Size: 3741 373 3063
Fragments arranged by size:
3741 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3173 3273 3529 4399 5240 6624 6892 1632
Size: 1541 100 256 870 841 1384 268 1917
Fragments arranged by size:
1917 1541 1384 870 841 268 256 100
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6980
AflIII A'CryG_T
Cuts at: 1203 6076 7053 1203
Size: 4873 977 1327
Fragments arranged by size:
4873 1327 977
AgeI A'CCGG_T
Cuts at: 2660 6008 2660
Size: 3348 3829
AhdI GACnn_n'nnGTC
Cuts at: 5906 5906
Size: 7177
AleI CACnn'nnGTG
Cuts at: 1166 2716 4248 1166
Size: 1550 1532 4095
Fragments arranged by size:
4095 1550 1532
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7177
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3641 3673 3641
Size: 32 7145
AlwNI CAG_nnn'CTG
Cuts at: 2041 2362 3088 3995 4920 6492 2041
Size: 321 726 907 925 1572 2726
Fragments arranged by size:
2726 1572 925 907 726 321
ApaLI G'TGCA_C
Cuts at: 5234 6390 5234
Size: 1156 6021
ApoI r'AATT_y
Cuts at: 1493 4438 4486 4712 4864 6973 1493
Size: 2945 48 226 152 2109 1697
Fragments arranged by size:
2945 2109 1697 226 152 48
AseI AT'TA_AT
Cuts at: 160 1032 5731 160
Size: 872 4699 1606
Fragments arranged by size:
4699 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7177
AvaI C'yCGr_G
Cuts at: 1870 1943 2779 4502 1870
Size: 73 836 1723 4545
Fragments arranged by size:
4545 1723 836 73
BamHI G'GATC_C
Cuts at: 4507 4874 4507
Size: 367 6810
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2561 2864 3893 4131 4149
Size: 325 217 627 774 303 1029 238 18
Cuts at: 4149 4498 5953 618
Size: 349 1455 1842
Fragments arranged by size:
1842 1455 1029 774 627 349 325 303
238 217 18
BanII G_rGCy'C
Cuts at: 1391 2694 3000 3543 4496 1391
Size: 1303 306 543 953 4072
Fragments arranged by size:
4072 1303 953 543 306
BbeI G_GCGC'C
Cuts at: 4135 4153 4135
Size: 18 7159
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2460 3502 928
Size: 971 561 1042 4603
Fragments arranged by size:
4603 1042 971 561
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5368 5402 1903
Size: 34 3431 34 3678
Fragments arranged by size:
3678 3431 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2166 2413 3643 5069 6279 2166
Size: 247 1230 1426 1210 3064
Fragments arranged by size:
3064 1426 1230 1210 247
BclI T'GATC_A
Cuts at: 1548 2503 2616 1548
Size: 955 113 6109
Fragments arranged by size:
6109 955 113
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4067 5788 136
Size: 107 122 71 631 605 2395 1721 1525
Fragments arranged by size:
2395 1721 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2217 7172 2217
Size: 4955 2222
BlpI GC'TnA_GC
Cuts at: 3578 4594 3578
Size: 1016 6161
Bme1580I G_kGCm'C
Cuts at: 1790 2354 2484 2564 5238 6394 1790
Size: 564 130 80 2674 1156 2573
Fragments arranged by size:
2674 2573 1156 564 130 80
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7177
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3310 4149 4908
Size: 416 53 83 399 24 1893 839 759
Cuts at: 4908 5866 442
Size: 958 1753
Fragments arranged by size:
1893 1753 958 839 759 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7177
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2292 5837 1239
Size: 1053 3545 2579
Fragments arranged by size:
3545 2579 1053
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5302 6167 6465 5302
Size: 865 298 6014
Fragments arranged by size:
6014 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2840 5840 882
Size: 617 1341 3000 2219
Fragments arranged by size:
3000 2219 1341 617
BsaAI yAC'GTr
Cuts at: 493 1204 2479 4474 4968 493
Size: 711 1275 1995 494 2702
Fragments arranged by size:
2702 1995 1275 711 494
BsaBI GATnn'nnATC
Cuts at: 3630 7171 3630
Size: 3541 3636
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3063 3132 3194 3570
Size: 53 83 186 936 1530 69 62 376
Cuts at: 3570 4070 4132 4150 4346 4984 5366 6001 275
Size: 500 62 18 196 638 382 635 1451
Fragments arranged by size:
1530 1451 936 638 635 500 382 376
196 186 83 69 62 62 53 18
BsaWI w'CCGG_w
Cuts at: 2660 5610 6008 6282 6429 6840 2660
Size: 2950 398 274 147 411 2997
Fragments arranged by size:
2997 2950 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6952
Fragments arranged by size:
6952 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2741 4067 1186
Size: 1555 1326 4296
Fragments arranged by size:
4296 1555 1326
BseYI C'CCAG_C
Cuts at: 2355 2786 2880 3127 3467 3471 3937 6380 6876
Size: 431 94 247 340 4 466 2443 496
Cuts at: 6876 2355
Size: 2656
Fragments arranged by size:
2656 2443 496 466 431 340 247 94
4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3383 1163
Size: 573 1647 4957
Fragments arranged by size:
4957 1647 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4322 4627 5388 6416 1540
Size: 275 186 2321 305 761 1028 2301
Fragments arranged by size:
2321 2301 1028 761 305 275 186
BsiHKAI G_wGCw'C
Cuts at: 3000 3543 5238 5323 6394 3000
Size: 543 1695 85 1071 3783
Fragments arranged by size:
3783 1695 1071 543 85
BsmI GAATG_Cn'
Cuts at: 2975 4688 4781 2975
Size: 1713 93 5371
Fragments arranged by size:
5371 1713 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3317 3317
Size: 7177
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2354 2484 2564 2694 3000 3543 4496
Size: 399 564 130 80 130 306 543 953
Cuts at: 4496 5238 5323 6394 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 953 742 564 543 399 306
130 130 85 80
BspEI T'CCGG_A
Cuts at: 6840 6840
Size: 7177
BspHI T'CATG_A
Cuts at: 2500 3591 5066 6796 2500
Size: 1091 1475 1730 2881
Fragments arranged by size:
2881 1730 1475 1091
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3405 4295 4532 844
Size: 2561 890 237 3489
Fragments arranged by size:
3489 2561 890 237
BsrBI CCG'CTC
Cuts at: 1394 1823 2862 5064 1394
Size: 429 1039 2202 3507
Fragments arranged by size:
3507 2202 1039 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4056 5672 5846 65
Size: 1183 2808 1616 174 1396
Fragments arranged by size:
2808 1616 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2564 2660 2899 3046 4317 5821 6008
Size: 110 754 96 239 147 1271 1504 187
Cuts at: 6008 1700
Size: 2869
Fragments arranged by size:
2869 1504 1271 754 239 187 147 110
96
BsrGI T'GTAC_A
Cuts at: 96 3291 96
Size: 3195 3982
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7177
BssSI C'ACGA_G
Cuts at: 4244 5237 6249 4244
Size: 993 1012 5172
Fragments arranged by size:
5172 1012 993
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2389 4396 794
Size: 1595 2007 3575
Fragments arranged by size:
3575 2007 1595
BstBI TT'CG_AA
Cuts at: 2007 5981 2007
Size: 3974 3203
BstEII G'GTnAC_C
Cuts at: 1184 2663 1184
Size: 1479 5698
BstXI CCAn_nnnn'nTGG
Cuts at: 2962 3229 2962
Size: 267 6910
BstYI r'GATC_y
Cuts at: 1972 2217 2372 2546 3084 4507 4874 5259 5276
Size: 245 155 174 538 1423 367 385 17
Cuts at: 5276 6728 6814 6826 7172 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1423 538 385 367 346 245
174 155 86 17 12
BstZ17I GTA'TAC
Cuts at: 4471 4471
Size: 7177
Bsu36I CC'TnA_GG
Cuts at: 3645 3996 3645
Size: 351 6826
BtgI C'CryG_G
Cuts at: 513 1065 2484 2539 2955 3381 3487 3585 4447
Size: 552 1419 55 416 426 106 98 862
Cuts at: 4447 513
Size: 3243
Fragments arranged by size:
3243 1419 862 552 426 416 106 98
55
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6438
Fragments arranged by size:
6438 683 56
BtsI GCAGTG_nn'
Cuts at: 2711 2892 3445 4693 5487 5507 2711
Size: 181 553 1248 794 20 4381
Fragments arranged by size:
4381 1248 794 553 181 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7177
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3762 3797 529
Size: 35 3198 35 3909
Fragments arranged by size:
3909 3198 35 35
DraI TTT'AAA
Cuts at: 4481 4481
Size: 7177
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3062 4007 6184 809
Size: 507 1746 945 2177 1802
Fragments arranged by size:
2177 1802 1746 945 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2901 3021 3384
Size: 54 1425 50 275 18 1071 120 363
Cuts at: 3384 3920 4624 5513 7142 8
Size: 536 704 889 1629 43
Fragments arranged by size:
1629 1425 1071 889 704 536 363 275
120 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4624 1537
Size: 275 2812 4090
Fragments arranged by size:
4090 2812 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2843 3548 4085 4197 5107 1353
Size: 1490 705 537 112 910 3423
Fragments arranged by size:
3423 1490 910 705 537 112
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3346 5764 6138 6284 387
Size: 1064 138 1757 2418 374 146 1280
Fragments arranged by size:
2418 1757 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2998 3541 2998
Size: 543 6634
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2292 3173 3273 3529 4399 5240 5837
Size: 393 660 881 100 256 870 841 597
Cuts at: 5837 6624 6892 1239
Size: 787 268 1524
Fragments arranged by size:
1524 881 870 841 787 660 597 393
268 256 100
EcoO109I rG'GnC_Cy
Cuts at: 2191 2248 2752 2790 3499 3976 3992 2191
Size: 57 504 38 709 477 16 5376
Fragments arranged by size:
5376 709 504 477 57 38 16
EcoRI G'AATT_C
Cuts at: 4486 4864 4486
Size: 378 6799
EcoRV GAT'ATC
Cuts at: 4183 4183
Size: 7177
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2477 2477
Size: 7177
FspI TGC'GCA
Cuts at: 5683 5683
Size: 7177
HaeII r_GCGC'y
Cuts at: 1678 3585 4109 4135 4153 6324 1678
Size: 1907 524 26 18 2171 2531
Fragments arranged by size:
2531 2171 1907 524 26 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2209 2241 2357 2389 2754
Size: 32 135 32 637 32 116 32 365
Cuts at: 2754 2786 3863 3895 4196 4198 4228 4230 4238
Size: 32 1077 32 301 2 30 2 8
Cuts at: 4238 4270 5818 5850 5892 5924 1373
Size: 32 1548 32 42 32 2626
Fragments arranged by size:
2626 1548 1077 637 365 301 135 116
42 32 32 32 32 32 32 32
32 32 30 8 2 2
HincII GTy'rAC
Cuts at: 669 1657 1938 2109 2167 2811 3136 4521 4767
Size: 988 281 171 58 644 325 1385 246
Cuts at: 4767 669
Size: 3079
Fragments arranged by size:
3079 1385 988 644 325 281 246 171
58
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7177
HpaI GTT'AAC
Cuts at: 2109 4767 2109
Size: 2658 4519
KasI G'GCGC_C
Cuts at: 4131 4149 4131
Size: 18 7159
KpnI G_GTAC'C
Cuts at: 4502 4502
Size: 7177
MfeI C'AATT_G
Cuts at: 4776 4776
Size: 7177
MluI A'CGCG_T
Cuts at: 7053 7053
Size: 7177
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3246 3774 6291 6475 1305
Size: 1941 528 2517 184 2007
Fragments arranged by size:
2517 2007 1941 528 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3386 3922 10
Size: 54 1425 343 1554 536 3265
Fragments arranged by size:
3265 1554 1425 536 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2364 2477 2716 2960 3492 3521 4248
Size: 648 1198 113 239 244 532 29 727
Cuts at: 4248 4361 5135 5494 5653 518
Size: 113 774 359 159 2042
Fragments arranged by size:
2042 1198 774 727 648 532 359 244
239 159 113 113 29
MspA1I CmG'CkG
Cuts at: 1606 2880 3471 4231 4593 5270 6418 6663 1606
Size: 1274 591 760 362 677 1148 245 2120
Fragments arranged by size:
2120 1274 1148 760 677 591 362 245
NaeI GCC'GGC
Cuts at: 2901 2901
Size: 7177
NarI GG'CG_CC
Cuts at: 4132 4150 4132
Size: 18 7159
NcoI C'CATG_G
Cuts at: 513 1065 2539 3487 513
Size: 552 1474 948 4203
Fragments arranged by size:
4203 1474 948 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7177
NgoMIV G'CCGG_C
Cuts at: 2899 2899
Size: 7177
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7177
NotI GC'GGCC_GC
Cuts at: 4624 4624
Size: 7177
NspI r_CATG'y
Cuts at: 1132 1229 2392 2947 4535 6080 1132
Size: 97 1163 555 1588 1545 2229
Fragments arranged by size:
2229 1588 1545 1163 555 97
PasI CC'CwG_GG
Cuts at: 1348 2035 1348
Size: 687 6490
PciI A'CATG_T
Cuts at: 6076 6076
Size: 7177
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2362 3370 3918 1108
Size: 1254 1008 548 4367
Fragments arranged by size:
4367 1254 1008 548
PfoI T'CCnGG_A
Cuts at: 2309 2694 2309
Size: 385 6792
PmeI GTTT'AAAC
Cuts at: 4481 4481
Size: 7177
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5246 5278 6759 6791 5246
Size: 32 1481 32 5632
Fragments arranged by size:
5632 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2248 2752 3499 3976 2248
Size: 504 747 477 5449
Fragments arranged by size:
5449 747 504 477
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7177
PsiI TTA'TAA
Cuts at: 4747 4747
Size: 7177
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2972 3004 2972
Size: 32 7145
PstI C_TGCA'G
Cuts at: 830 4221 4263 4529 830
Size: 3391 42 266 3478
Fragments arranged by size:
3478 3391 266 42
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7177
PvuII CAG'CTG
Cuts at: 2880 3471 4231 2880
Size: 591 760 5826
Fragments arranged by size:
5826 760 591
SacI G_AGCT'C
Cuts at: 3000 3543 3000
Size: 543 6634
SalI G'TCGA_C
Cuts at: 1936 4519 1936
Size: 2583 4594
SanDI GG'GwC_CC
Cuts at: 2248 2752 2248
Size: 504 6673
SapI GCTCTTCn'nnn_
Cuts at: 3548 3548
Size: 7177
SbfI CC_TGCA'GG
Cuts at: 4529 4529
Size: 7177
ScaI AGT'ACT
Cuts at: 2240 5425 7137 2240
Size: 3185 1712 2280
Fragments arranged by size:
3185 2280 1712
SfcI C'TryA_G
Cuts at: 826 1045 4217 4259 4525 5660 6341 6532 826
Size: 219 3172 42 266 1135 681 191 1471
Fragments arranged by size:
3172 1471 1135 681 266 219 191 42
SfoI GGC'GCC
Cuts at: 4133 4151 4133
Size: 18 7159
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7177
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7177
SmaI CCC'GGG
Cuts at: 1872 4504 1872
Size: 2632 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5281 6182 6444 820
Size: 197 926 3338 901 262 1553
Fragments arranged by size:
3338 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4474 4968 493
Size: 3981 494 2702
Fragments arranged by size:
3981 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7177
SphI G_CATG'C
Cuts at: 1132 4535 1132
Size: 3403 3774
SspI AAT'ATT
Cuts at: 5 52 5101 5
Size: 47 5049 2081
Fragments arranged by size:
5049 2081 47
StuI AGG'CCT
Cuts at: 1517 3150 1517
Size: 1633 5544
StyI C'CwwG_G
Cuts at: 513 1065 2539 3139 3487 4616 513
Size: 552 1474 600 348 1129 3074
Fragments arranged by size:
3074 1474 1129 600 552 348
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3358 3518 5204 5221 5374 5559 6004
Size: 1255 1778 160 1686 17 153 185 445
Cuts at: 6004 325
Size: 1498
Fragments arranged by size:
1778 1686 1498 1255 445 185 160 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2238 2907 3291
Size: 274 80 33 51 1443 261 669 384
Cuts at: 3291 4110 4433 5423 7135 96
Size: 819 323 990 1712 138
Fragments arranged by size:
1712 1443 990 819 669 384 323 274
261 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3844 3876 4351 4383 4439 4471 7137 7169 3844
Size: 32 475 32 56 32 2666 32 3852
Fragments arranged by size:
3852 2666 475 56 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2815 2930 1316
Size: 1499 115 5563
Fragments arranged by size:
5563 1499 115
XbaI T'CTAG_A
Cuts at: 4513 4513
Size: 7177
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2218 2218
Size: 7177
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7177
XmaI C'CCGG_G
Cuts at: 1870 4502 1870
Size: 2632 4545
XmnI GAAnn'nnTTC
Cuts at: 3801 5306 3801
Size: 1505 5672
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3064 3571 4985 276
Size: 53 83 186 936 1530 507 1414 2468
Fragments arranged by size:
2468 1530 1414 936 507 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PsrI PstI PvuI PvuII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI DraIII EcoNI FseI FspAI NruI NsiI
PacI PmlI PspOMI PspXI RsrII SacII SexAI SfiI
SrfI SwaI