(Circular) (Six-base) MAPSORT of: pFN21ASDA1259.seq Check: 8759 from: 1 to: 9397 pFN21ASDA1259 9397 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3754 3754 Size: 9397 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7207 278 Size: 53 83 186 936 5671 2468 Fragments arranged by size: 5671 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3752 6740 1937 Size: 1815 2988 4594 Fragments arranged by size: 4594 2988 1815 Acc65I G'GTAC_C Cuts at: 6718 6718 Size: 9397 AclI AA'CG_TT Cuts at: 1563 7524 7897 1563 Size: 5961 373 3063 Fragments arranged by size: 5961 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2072 2399 2754 2898 3398 4724 5156 7460 Size: 440 327 355 144 500 1326 432 2304 Cuts at: 7460 8844 9112 1632 Size: 1384 268 1917 Fragments arranged by size: 2304 1917 1384 1326 500 440 432 355 327 268 144 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9200 AflIII A'CryG_T Cuts at: 1203 6465 8296 9273 1203 Size: 5262 1831 977 1327 Fragments arranged by size: 5262 1831 1327 977 AgeI A'CCGG_T Cuts at: 8228 8228 Size: 9397 AhdI GACnn_n'nnGTC Cuts at: 8126 8126 Size: 9397 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 9397 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2859 6134 6176 6512 1184 Size: 1675 3275 42 336 4069 Fragments arranged by size: 4069 3275 1675 336 42 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4352 4384 6576 6608 4352 Size: 32 2192 32 7141 Fragments arranged by size: 7141 2192 32 32 AlwNI CAG_nnn'CTG Cuts at: 3376 4339 4950 7140 8712 3376 Size: 963 611 2190 1572 4061 Fragments arranged by size: 4061 2190 1572 963 611 ApaI G_GGCC'C Cuts at: 2107 5185 5653 2107 Size: 3078 468 5851 Fragments arranged by size: 5851 3078 468 ApaLI G'TGCA_C Cuts at: 7454 8610 7454 Size: 1156 8241 ApoI r'AATT_y Cuts at: 1493 2254 2356 2654 3797 4145 4365 6261 6706 Size: 761 102 298 1143 348 220 1896 445 Cuts at: 6706 6932 7084 9193 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1896 1697 1143 761 445 348 298 226 220 152 102 AseI AT'TA_AT Cuts at: 160 1032 3633 4668 7951 160 Size: 872 2601 1035 3283 1606 Fragments arranged by size: 3283 2601 1606 1035 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9397 AvaI C'yCGr_G Cuts at: 1870 1943 6204 6722 1870 Size: 73 4261 518 4545 Fragments arranged by size: 4545 4261 518 73 BamHI G'GATC_C Cuts at: 2221 6727 7094 2221 Size: 4506 367 4524 Fragments arranged by size: 4524 4506 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2039 2628 3768 5705 6668 Size: 325 217 627 252 589 1140 1937 963 Cuts at: 6668 6718 8173 618 Size: 50 1455 1842 Fragments arranged by size: 1937 1842 1455 1140 963 627 589 325 252 217 50 BanII G_rGCy'C Cuts at: 1391 2107 4102 4403 5185 5653 6716 1391 Size: 716 1995 301 782 468 1063 4072 Fragments arranged by size: 4072 1995 1063 782 716 468 301 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2457 3285 3872 4383 4570 5813 928 Size: 971 558 828 587 511 187 1243 4512 Fragments arranged by size: 4512 1243 971 828 587 558 511 187 BbvCI CC'TCA_GC Cuts at: 4253 6190 4253 Size: 1937 7460 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7588 7622 1903 Size: 34 5651 34 3678 Fragments arranged by size: 5651 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3260 7289 8499 3260 Size: 4029 1210 4158 Fragments arranged by size: 4158 4029 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 9397 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2993 3467 3692 Size: 107 122 71 631 605 1321 474 225 Cuts at: 3692 3701 8008 136 Size: 9 4307 1525 Fragments arranged by size: 4307 1525 1321 631 605 474 225 122 107 71 9 BglII A'GATC_T Cuts at: 5478 6099 9392 5478 Size: 621 3293 5483 Fragments arranged by size: 5483 3293 621 BlpI GC'TnA_GC Cuts at: 2867 6814 2867 Size: 3947 5450 Bme1580I G_kGCm'C Cuts at: 1790 2107 3031 5185 5653 7458 8614 1790 Size: 317 924 2154 468 1805 1156 2573 Fragments arranged by size: 2573 2154 1805 1156 924 468 317 BmgBI CAC'GTC Cuts at: 1318 6293 1318 Size: 4975 4422 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2206 3498 5573 Size: 416 53 83 399 24 789 1292 2075 Cuts at: 5573 7128 8086 442 Size: 1555 958 1753 Fragments arranged by size: 2075 1753 1555 1292 958 789 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9397 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2298 3877 4698 5262 5441 5577 6702 8057 Size: 1059 1579 821 564 179 136 1125 1355 Cuts at: 8057 1239 Size: 2579 Fragments arranged by size: 2579 1579 1355 1125 1059 821 564 179 136 Bpu10I CC'TnA_GC Cuts at: 3984 4253 5659 6190 3984 Size: 269 1406 531 7191 Fragments arranged by size: 7191 1406 531 269 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3977 5345 5352 7522 8387 8685 3977 Size: 1368 7 2170 865 298 4689 Fragments arranged by size: 4689 2170 1368 865 298 7 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4512 4650 8060 882 Size: 617 3013 138 3410 2219 Fragments arranged by size: 3410 3013 2219 617 138 BsaAI yAC'GTr Cuts at: 493 1204 6694 7188 493 Size: 711 5490 494 2702 Fragments arranged by size: 5490 2702 711 494 BsaBI GATnn'nnATC Cuts at: 4209 6104 9391 4209 Size: 1895 3287 4215 Fragments arranged by size: 4215 3287 1895 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 7204 7586 8221 275 Size: 53 83 186 936 5671 382 635 1451 Fragments arranged by size: 5671 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2109 6651 7830 8228 8502 8649 9060 2109 Size: 4542 1179 398 274 147 411 2446 Fragments arranged by size: 4542 2446 1179 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3306 3309 3336 3339 4492 Size: 30 165 30 1903 3 27 3 1153 Cuts at: 4492 4522 6113 6143 6170 6200 6202 6232 1178 Size: 30 1591 30 27 30 2 30 4343 Fragments arranged by size: 4343 1903 1591 1153 165 30 30 30 30 30 30 27 27 3 3 2 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3330 4270 4999 6178 1186 Size: 2144 940 729 1179 4405 Fragments arranged by size: 4405 2144 1179 940 729 BseYI C'CCAG_C Cuts at: 6181 8600 9096 6181 Size: 2419 496 6482 Fragments arranged by size: 6482 2419 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2044 2604 3947 5133 6104 1163 Size: 573 308 560 1343 1186 971 4456 Fragments arranged by size: 4456 1343 1186 971 573 560 308 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6847 7608 8636 1540 Size: 275 186 4846 761 1028 2301 Fragments arranged by size: 4846 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3093 7458 7543 8614 3093 Size: 4365 85 1071 3876 Fragments arranged by size: 4365 3876 1071 85 BsmI GAATG_Cn' Cuts at: 2406 2848 3388 3639 5721 6908 7001 2406 Size: 442 540 251 2082 1187 93 4802 Fragments arranged by size: 4802 2082 1187 540 442 251 93 BsmBI CGTCTCn'nnnn_ Cuts at: 6247 6247 Size: 9397 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2107 3031 3093 4102 4403 5185 5653 Size: 399 317 924 62 1009 301 782 468 Cuts at: 5653 6716 7458 7543 8614 1391 Size: 1063 742 85 1071 2174 Fragments arranged by size: 2174 1071 1063 1009 924 782 742 468 399 317 301 85 62 BspEI T'CCGG_A Cuts at: 6651 9060 6651 Size: 2409 6988 BspHI T'CATG_A Cuts at: 3216 3241 3829 4437 7286 9016 3216 Size: 25 588 608 2849 1730 3597 Fragments arranged by size: 3597 2849 1730 608 588 25 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3754 5665 6752 844 Size: 2910 1911 1087 3489 Fragments arranged by size: 3489 2910 1911 1087 BsrBI CCG'CTC Cuts at: 1394 1823 7284 1394 Size: 429 5461 3507 Fragments arranged by size: 5461 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2258 3681 5107 6405 7892 8066 65 Size: 1183 1010 1423 1426 1298 1487 174 1396 Fragments arranged by size: 1487 1426 1423 1396 1298 1183 1010 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5688 8041 8228 1700 Size: 110 3878 2353 187 2869 Fragments arranged by size: 3878 2869 2353 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9397 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9397 BssSI C'ACGA_G Cuts at: 3024 4590 5067 5550 7457 8469 3024 Size: 1566 477 483 1907 1012 3952 Fragments arranged by size: 3952 1907 1566 1012 483 477 BstAPI GCAn_nnn'nTGC Cuts at: 794 6389 794 Size: 5595 3802 BstBI TT'CG_AA Cuts at: 2007 8201 2007 Size: 6194 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9397 BstXI CCAn_nnnn'nTGG Cuts at: 5812 6139 5812 Size: 327 9070 BstYI r'GATC_y Cuts at: 1972 2221 4020 5478 6099 6727 7094 7479 7496 Size: 249 1799 1458 621 628 367 385 17 Cuts at: 7496 8948 9034 9046 9392 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1799 1458 1452 628 621 385 367 346 249 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3266 4853 3266 Size: 1587 7810 BtgI C'CryG_G Cuts at: 513 1065 2022 4083 5640 6378 513 Size: 552 957 2061 1557 738 3532 Fragments arranged by size: 3532 2061 1557 957 738 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8658 Fragments arranged by size: 8658 683 56 BtsI GCAGTG_nn' Cuts at: 4965 5085 5171 5577 6118 6160 6394 6913 7707 Size: 120 86 406 541 42 234 519 794 Cuts at: 7707 7727 4965 Size: 20 6635 Fragments arranged by size: 6635 794 541 519 406 234 120 86 42 20 ClaI AT'CG_AT Cuts at: 1554 3303 1554 Size: 1749 7648 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9362 DraI TTT'AAA Cuts at: 3364 6701 3364 Size: 3337 6060 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4982 6164 6647 8404 809 Size: 507 3666 1182 483 1757 1802 Fragments arranged by size: 3666 1802 1757 1182 507 483 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3609 4752 5058 Size: 54 1425 50 275 18 1779 1143 306 Cuts at: 5058 6844 7733 9362 8 Size: 1786 889 1629 43 Fragments arranged by size: 1786 1779 1629 1425 1143 889 306 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6844 1537 Size: 275 5032 4090 Fragments arranged by size: 5032 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2707 5738 5832 5865 6559 7327 1353 Size: 1354 3031 94 33 694 768 3423 Fragments arranged by size: 3423 3031 1354 768 694 94 33 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6472 7984 8358 8504 387 Size: 1064 138 4883 1512 374 146 1280 Fragments arranged by size: 4883 1512 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2072 2298 2399 2754 2898 3398 3877 Size: 393 440 226 101 355 144 500 479 Cuts at: 3877 4698 4724 5156 5262 5441 5577 6702 7460 Size: 821 26 432 106 179 136 1125 758 Cuts at: 7460 8057 8844 9112 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1125 821 787 758 597 500 479 440 432 393 355 268 226 179 144 136 106 101 26 EcoNI CCTnn'n_nnAGG Cuts at: 3041 4792 5420 5455 3041 Size: 1751 628 35 6983 Fragments arranged by size: 6983 1751 628 35 EcoO109I rG'GnC_Cy Cuts at: 2103 2104 3373 3725 4065 5181 5182 5380 5649 Size: 1 1269 352 340 1116 1 198 269 Cuts at: 5649 2103 Size: 5851 Fragments arranged by size: 5851 1269 1116 352 340 269 198 1 1 EcoRI G'AATT_C Cuts at: 6706 7084 6706 Size: 378 9019 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3034 4042 6570 3034 Size: 1008 2528 5861 Fragments arranged by size: 5861 2528 1008 FspI TGC'GCA Cuts at: 7903 7903 Size: 9397 HaeII r_GCGC'y Cuts at: 1678 3992 4826 6552 8544 1678 Size: 2314 834 1726 1992 2531 Fragments arranged by size: 2531 2314 1992 1726 834 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5027 5059 5456 5488 5553 Size: 32 135 32 3455 32 397 32 65 Cuts at: 5553 5585 6170 6202 8038 8070 8112 8144 1373 Size: 32 585 32 1836 32 42 32 2626 Fragments arranged by size: 3455 2626 1836 585 397 135 65 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3328 6741 6987 669 Size: 988 281 1390 3413 246 3079 Fragments arranged by size: 3413 3079 1390 988 281 246 HindIII A'AGCT_T Cuts at: 748 2910 2948 3114 748 Size: 2162 38 166 7031 Fragments arranged by size: 7031 2162 166 38 HpaI GTT'AAC Cuts at: 3328 6987 3328 Size: 3659 5738 KpnI G_GTAC'C Cuts at: 6722 6722 Size: 9397 MfeI C'AATT_G Cuts at: 3763 6996 3763 Size: 3233 6164 MluI A'CGCG_T Cuts at: 9273 9273 Size: 9397 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2094 3114 3995 4022 4663 5823 6054 8511 Size: 789 1020 881 27 641 1160 231 2457 Cuts at: 8511 8695 1305 Size: 184 2007 Fragments arranged by size: 2457 2007 1160 1020 881 789 641 231 184 27 MscI TGG'CCA Cuts at: 10 64 1489 1832 3611 4754 5060 10 Size: 54 1425 343 1779 1143 306 4347 Fragments arranged by size: 4347 1779 1425 1143 343 306 54 MslI CAynn'nnrTG Cuts at: 518 1166 5531 6065 7355 7714 7873 518 Size: 648 4365 534 1290 359 159 2042 Fragments arranged by size: 4365 2042 1290 648 534 359 159 MspA1I CmG'CkG Cuts at: 1606 2839 3127 3458 3919 3949 4914 4993 5240 Size: 1233 288 331 461 30 965 79 247 Cuts at: 5240 5758 6284 6428 6440 6813 7490 8638 8883 Size: 518 526 144 12 373 677 1148 245 Cuts at: 8883 1606 Size: 2120 Fragments arranged by size: 2120 1233 1148 965 677 526 518 461 373 331 288 247 245 144 79 30 12 NcoI C'CATG_G Cuts at: 513 1065 2022 4083 5640 513 Size: 552 957 2061 1557 4270 Fragments arranged by size: 4270 2061 1557 957 552 NdeI CA'TA_TG Cuts at: 387 2602 387 Size: 2215 7182 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9397 NotI GC'GGCC_GC Cuts at: 6844 6844 Size: 9397 NspI r_CATG'y Cuts at: 1132 1229 2503 6163 6755 8300 1132 Size: 97 1274 3660 592 1545 2229 Fragments arranged by size: 3660 2229 1545 1274 592 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 9397 PciI A'CATG_T Cuts at: 8296 8296 Size: 9397 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 9397 PfoI T'CCnGG_A Cuts at: 3252 3252 Size: 9397 PmeI GTTT'AAAC Cuts at: 6701 6701 Size: 9397 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2460 2492 7466 7498 8979 9011 2460 Size: 32 4974 32 1481 32 2846 Fragments arranged by size: 4974 2846 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3725 4065 5380 3725 Size: 340 1315 7742 Fragments arranged by size: 7742 1315 340 PshAI GACnn'nnGTC Cuts at: 1613 2134 2768 1613 Size: 521 634 8242 Fragments arranged by size: 8242 634 521 PsiI TTA'TAA Cuts at: 6967 6967 Size: 9397 PspOMI G'GGCC_C Cuts at: 2103 5181 5649 2103 Size: 3078 468 5851 Fragments arranged by size: 5851 3078 468 PstI C_TGCA'G Cuts at: 830 5200 5587 6289 6427 6439 6501 6749 830 Size: 4370 387 702 138 12 62 248 3478 Fragments arranged by size: 4370 3478 702 387 248 138 62 12 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9397 PvuII CAG'CTG Cuts at: 2839 3458 3919 4914 5240 6284 6440 2839 Size: 619 461 995 326 1044 156 5796 Fragments arranged by size: 5796 1044 995 619 461 326 156 SalI G'TCGA_C Cuts at: 1936 6739 1936 Size: 4803 4594 SanDI GG'GwC_CC Cuts at: 4065 4065 Size: 9397 SbfI CC_TGCA'GG Cuts at: 6749 6749 Size: 9397 ScaI AGT'ACT Cuts at: 2093 2576 7645 9357 2093 Size: 483 5069 1712 2133 Fragments arranged by size: 5069 2133 1712 483 SexAI A'CCwGG_T Cuts at: 4977 5994 4977 Size: 1017 8380 SfcI C'TryA_G Cuts at: 826 1045 2327 2578 4792 5196 5583 6285 6423 Size: 219 1282 251 2214 404 387 702 138 Cuts at: 6423 6435 6497 6745 7880 8561 8752 826 Size: 12 62 248 1135 681 191 1471 Fragments arranged by size: 2214 1471 1282 1135 702 681 404 387 251 248 219 191 138 62 12 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9397 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9397 SmaI CCC'GGG Cuts at: 1872 6724 1872 Size: 4852 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3992 5360 5367 6204 7501 8402 Size: 197 926 2049 1368 7 837 1297 901 Cuts at: 8402 8664 820 Size: 262 1553 Fragments arranged by size: 2049 1553 1368 1297 926 901 837 262 197 7 SnaBI TAC'GTA Cuts at: 493 6694 7188 493 Size: 6201 494 2702 Fragments arranged by size: 6201 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 9397 SphI G_CATG'C Cuts at: 1132 6755 1132 Size: 5623 3774 SspI AAT'ATT Cuts at: 5 52 2158 7321 5 Size: 47 2106 5163 2081 Fragments arranged by size: 5163 2106 2081 47 StuI AGG'CCT Cuts at: 1517 2589 1517 Size: 1072 8325 StyI C'CwwG_G Cuts at: 513 1065 2022 3042 3661 4083 4164 5640 5736 Size: 552 957 1020 619 422 81 1476 96 Cuts at: 5736 6836 513 Size: 1100 3074 Fragments arranged by size: 3074 1476 1100 1020 957 619 552 422 96 81 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2027 5121 7424 7441 7594 7779 8224 Size: 1255 447 3094 2303 17 153 185 445 Cuts at: 8224 325 Size: 1498 Fragments arranged by size: 3094 2303 1498 1255 447 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2091 2574 3057 Size: 274 80 33 51 1443 114 483 483 Cuts at: 3057 3673 7643 9355 96 Size: 616 3970 1712 138 Fragments arranged by size: 3970 1712 1443 616 483 483 274 138 114 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5211 5243 6113 6145 9357 9389 5211 Size: 32 870 32 3212 32 5219 Fragments arranged by size: 5219 3212 870 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3421 6294 1316 Size: 2105 2873 4419 Fragments arranged by size: 4419 2873 2105 XbaI T'CTAG_A Cuts at: 5827 6733 5827 Size: 906 8491 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3916 3916 Size: 9397 XhoI C'TCGA_G Cuts at: 1943 6204 1943 Size: 4261 5136 XmaI C'CCGG_G Cuts at: 1870 6722 1870 Size: 4852 4545 XmnI GAAnn'nnTTC Cuts at: 2490 5721 7526 2490 Size: 3231 1805 4361 Fragments arranged by size: 4361 3231 1805 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7205 276 Size: 53 83 186 936 5671 2468 Fragments arranged by size: 5671 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SapI SfiI SfoI SrfI SwaI