Restriction map for
pFN21ASDA1258
(Circular) (Six-base) MAPSORT of: pFN21ASDA1258.seq Check: 2286 from: 1 to: 6091
pFN21ASDA1258 6091 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3349 3349
Size: 6091
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3901 278
Size: 53 83 186 936 2365 2468
Fragments arranged by size:
2468 2365 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3434 1937
Size: 1497 4594
Acc65I G'GTAC_C
Cuts at: 3412 3412
Size: 6091
AclI AA'CG_TT
Cuts at: 1563 4218 4591 1563
Size: 2655 373 3063
Fragments arranged by size:
3063 2655 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3112 4154 5538 5806 1632
Size: 1480 1042 1384 268 1917
Fragments arranged by size:
1917 1480 1384 1042 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5894
AflIII A'CryG_T
Cuts at: 1203 4990 5967 1203
Size: 3787 977 1327
Fragments arranged by size:
3787 1327 977
AgeI A'CCGG_T
Cuts at: 4922 4922
Size: 6091
AhdI GACnn_n'nnGTC
Cuts at: 4820 4820
Size: 6091
AleI CACnn'nnGTG
Cuts at: 1166 2810 1166
Size: 1644 4447
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2327 2878 1184
Size: 1143 551 4397
Fragments arranged by size:
4397 1143 551
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2346 2378 2346
Size: 32 6059
AlwNI CAG_nnn'CTG
Cuts at: 3834 5406 3834
Size: 1572 4519
ApaLI G'TGCA_C
Cuts at: 4148 5304 4148
Size: 1156 4935
ApoI r'AATT_y
Cuts at: 1493 2498 3203 3400 3626 3778 5887 1493
Size: 1005 705 197 226 152 2109 1697
Fragments arranged by size:
2109 1697 1005 705 226 197 152
AseI AT'TA_AT
Cuts at: 160 1032 3087 4645 160
Size: 872 2055 1558 1606
Fragments arranged by size:
2055 1606 1558 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6091
AvaI C'yCGr_G
Cuts at: 1870 1943 3416 1870
Size: 73 1473 4545
Fragments arranged by size:
4545 1473 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2997 3030 2997
Size: 33 6058
BamHI G'GATC_C
Cuts at: 3421 3788 3421
Size: 367 5724
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3412 4867 618
Size: 325 217 627 1625 1455 1842
Fragments arranged by size:
1842 1625 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3410 1391
Size: 2019 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3118 928
Size: 971 1219 3901
Fragments arranged by size:
3901 1219 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4282 4316 1903
Size: 34 2345 34 3678
Fragments arranged by size:
3678 2345 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3983 5193 3983
Size: 1210 4881
BclI T'GATC_A
Cuts at: 1548 3219 1548
Size: 1671 4420
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4702 136
Size: 107 122 71 631 605 3030 1525
Fragments arranged by size:
3030 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6086 6086
Size: 6091
BlpI GC'TnA_GC
Cuts at: 2945 3508 2945
Size: 563 5528
Bme1580I G_kGCm'C
Cuts at: 1790 4152 5308 1790
Size: 2362 1156 2573
Fragments arranged by size:
2573 2362 1156
BmgBI CAC'GTC
Cuts at: 1318 3013 1318
Size: 1695 4396
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2708 2784 3822
Size: 416 53 83 399 24 1291 76 1038
Cuts at: 3822 4780 442
Size: 958 1753
Fragments arranged by size:
1753 1291 1038 958 416 399 83 76
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6091
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4751 1239
Size: 3512 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4216 5081 5379 4216
Size: 865 298 4928
Fragments arranged by size:
4928 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2561 4754 882
Size: 617 1062 2193 2219
Fragments arranged by size:
2219 2193 1062 617
BsaAI yAC'GTr
Cuts at: 493 1204 3388 3882 493
Size: 711 2184 494 2702
Fragments arranged by size:
2702 2184 711 494
BsaBI GATnn'nnATC
Cuts at: 6085 6085
Size: 6091
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3898 4280 4915 275
Size: 53 83 186 936 2365 382 635 1451
Fragments arranged by size:
2365 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4524 4922 5196 5343 5754 4524
Size: 398 274 147 411 4861
Fragments arranged by size:
4861 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5866
Fragments arranged by size:
5866 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2111 2919 1186
Size: 925 808 4358
Fragments arranged by size:
4358 925 808
BseYI C'CCAG_C
Cuts at: 3001 5294 5790 3001
Size: 2293 496 3302
Fragments arranged by size:
3302 2293 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5518
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3541 4302 5330 1540
Size: 275 186 1540 761 1028 2301
Fragments arranged by size:
2301 1540 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4152 4237 5308 4152
Size: 85 1071 4935
Fragments arranged by size:
4935 1071 85
BsmI GAATG_Cn'
Cuts at: 3602 3695 3602
Size: 93 5998
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3410 4152 4237 5308 1391
Size: 399 1620 742 85 1071 2174
Fragments arranged by size:
2174 1620 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5754 5754
Size: 6091
BspHI T'CATG_A
Cuts at: 3980 5710 3980
Size: 1730 4361
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2099 2319 3349 3446 844
Size: 1255 220 1030 97 3489
Fragments arranged by size:
3489 1255 1030 220 97
BsrBI CCG'CTC
Cuts at: 1394 1823 2033 3978 1394
Size: 429 210 1945 3507
Fragments arranged by size:
3507 1945 429 210
BsrDI GCAATG_nn'
Cuts at: 65 1248 2706 4586 4760 65
Size: 1183 1458 1880 174 1396
Fragments arranged by size:
1880 1458 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4735 4922 1700
Size: 110 2925 187 2869
Fragments arranged by size:
2925 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6091
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6091
BssSI C'ACGA_G
Cuts at: 4151 5163 4151
Size: 1012 5079
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2529 794
Size: 1735 4356
BstBI TT'CG_AA
Cuts at: 2007 4895 2007
Size: 2888 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6091
BstYI r'GATC_y
Cuts at: 1972 3421 3788 4173 4190 5642 5728 5740 6086
Size: 1449 367 385 17 1452 86 12 346
Cuts at: 6086 1972
Size: 1977
Fragments arranged by size:
1977 1452 1449 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2097 513
Size: 552 1032 4507
Fragments arranged by size:
4507 1032 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2899 487
Size: 683 56 1673 3679
Fragments arranged by size:
3679 1673 683 56
BtsI GCAGTG_nn'
Cuts at: 2956 3066 3607 4401 4421 2956
Size: 110 541 794 20 4626
Fragments arranged by size:
4626 794 541 110 20
ClaI AT'CG_AT
Cuts at: 1554 2582 1554
Size: 1028 5063
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6056
DraI TTT'AAA
Cuts at: 3395 3395
Size: 6091
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5098 809
Size: 507 3782 1802
Fragments arranged by size:
3782 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2184 3538 4427
Size: 54 1425 50 275 18 354 1354 889
Cuts at: 4427 6056 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1354 889 354 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3538 1537
Size: 275 1726 4090
Fragments arranged by size:
4090 1726 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3049 3330 4021 1353
Size: 1696 281 691 3423
Fragments arranged by size:
3423 1696 691 281
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3366 4678 5052 5198 387
Size: 1064 138 1777 1312 374 146 1280
Fragments arranged by size:
1777 1312 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3112 4154 4751 5538 5806 1239
Size: 393 1480 1042 597 787 268 1524
Fragments arranged by size:
1524 1480 1042 787 597 393 268
EcoRI G'AATT_C
Cuts at: 3400 3778 3400
Size: 378 5713
FspI TGC'GCA
Cuts at: 4597 4597
Size: 6091
HaeII r_GCGC'y
Cuts at: 1678 5238 1678
Size: 3560 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2898 2930 4732 4764 4806
Size: 32 135 32 1326 32 1802 32 42
Cuts at: 4806 4838 1373
Size: 32 2626
Fragments arranged by size:
2626 1802 1326 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3435 3681 669
Size: 988 281 1497 246 3079
Fragments arranged by size:
3079 1497 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6091
HpaI GTT'AAC
Cuts at: 3681 3681
Size: 6091
KpnI G_GTAC'C
Cuts at: 3416 3416
Size: 6091
MfeI C'AATT_G
Cuts at: 3690 3690
Size: 6091
MluI A'CGCG_T
Cuts at: 5967 5967
Size: 6091
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3261 5205 5389 1305
Size: 1956 1944 184 2007
Fragments arranged by size:
2007 1956 1944 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2186 10
Size: 54 1425 343 354 3915
Fragments arranged by size:
3915 1425 354 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2102 2271 2745 2810 4049 4408 4567
Size: 648 936 169 474 65 1239 359 159
Cuts at: 4567 518
Size: 2042
Fragments arranged by size:
2042 1239 936 648 474 359 169 159
65
MspA1I CmG'CkG
Cuts at: 1606 2139 2509 3507 4184 5332 5577 1606
Size: 533 370 998 677 1148 245 2120
Fragments arranged by size:
2120 1148 998 677 533 370 245
NcoI C'CATG_G
Cuts at: 513 1065 2097 513
Size: 552 1032 4507
Fragments arranged by size:
4507 1032 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6091
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6091
NotI GC'GGCC_GC
Cuts at: 3538 3538
Size: 6091
NspI r_CATG'y
Cuts at: 1132 1229 3449 4994 1132
Size: 97 2220 1545 2229
Fragments arranged by size:
2229 2220 1545 97
PacI TTA_AT'TAA
Cuts at: 3087 3087
Size: 6091
PasI CC'CwG_GG
Cuts at: 1348 3160 1348
Size: 1812 4279
PciI A'CATG_T
Cuts at: 4990 4990
Size: 6091
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2193 3160 1108
Size: 1085 967 4039
Fragments arranged by size:
4039 1085 967
PmeI GTTT'AAAC
Cuts at: 3395 3395
Size: 6091
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4160 4192 5673 5705 4160
Size: 32 1481 32 4546
Fragments arranged by size:
4546 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2072 1613
Size: 459 5632
PsiI TTA'TAA
Cuts at: 2796 3661 2796
Size: 865 5226
PstI C_TGCA'G
Cuts at: 830 2353 2878 3443 830
Size: 1523 525 565 3478
Fragments arranged by size:
3478 1523 565 525
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6091
SalI G'TCGA_C
Cuts at: 1936 3433 1936
Size: 1497 4594
SapI GCTCTTCn'nnn_
Cuts at: 3049 3049
Size: 6091
SbfI CC_TGCA'GG
Cuts at: 3443 3443
Size: 6091
ScaI AGT'ACT
Cuts at: 4339 6051 4339
Size: 1712 4379
SfcI C'TryA_G
Cuts at: 826 1045 2349 2874 3439 4574 5255 5446 826
Size: 219 1304 525 565 1135 681 191 1471
Fragments arranged by size:
1471 1304 1135 681 565 525 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6091
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6091
SmaI CCC'GGG
Cuts at: 1872 3418 1872
Size: 1546 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4195 5096 5358 820
Size: 197 926 2252 901 262 1553
Fragments arranged by size:
2252 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3388 3882 493
Size: 2895 494 2702
Fragments arranged by size:
2895 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6091
SphI G_CATG'C
Cuts at: 1132 3449 1132
Size: 2317 3774
SspI AAT'ATT
Cuts at: 5 52 3331 4015 5
Size: 47 3279 684 2081
Fragments arranged by size:
3279 2081 684 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6091
StyI C'CwwG_G
Cuts at: 513 1065 2097 3530 513
Size: 552 1032 1433 3074
Fragments arranged by size:
3074 1433 1032 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2681 4118 4135 4288 4473 4918 325
Size: 1255 1101 1437 17 153 185 445 1498
Fragments arranged by size:
1498 1437 1255 1101 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2337 4337 6049
Size: 274 80 33 51 1443 360 2000 1712
Cuts at: 6049 96
Size: 138
Fragments arranged by size:
2000 1712 1443 360 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3345 3377 6051 6083 3345
Size: 32 2674 32 3353
Fragments arranged by size:
3353 2674 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6091
XbaI T'CTAG_A
Cuts at: 2633 3427 2633
Size: 794 5297
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2322 2322
Size: 6091
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6091
XmaI C'CCGG_G
Cuts at: 1870 3416 1870
Size: 1546 4545
XmnI GAAnn'nnTTC
Cuts at: 2891 4220 2891
Size: 1329 4762
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3899 276
Size: 53 83 186 936 2365 2468
Fragments arranged by size:
2468 2365 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PacI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI