Restriction map for
pFN21ASDA1252
(Circular) (Six-base) MAPSORT of: pFN21ASDA1252.seq Check: 6042 from: 1 to: 6433
pFN21ASDA1252 6433 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4243 278
Size: 53 83 186 936 2707 2468
Fragments arranged by size:
2707 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2085 2964 3776 1937
Size: 148 879 812 4594
Fragments arranged by size:
4594 879 812 148
Acc65I G'GTAC_C
Cuts at: 3754 3754
Size: 6433
AclI AA'CG_TT
Cuts at: 1563 4560 4933 1563
Size: 2997 373 3063
Fragments arranged by size:
3063 2997 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2940 4496 5880 6148 1632
Size: 1308 1556 1384 268 1917
Fragments arranged by size:
1917 1556 1384 1308 268
AflII C'TTAA_G
Cuts at: 820 1017 2411 820
Size: 197 1394 4842
Fragments arranged by size:
4842 1394 197
AflIII A'CryG_T
Cuts at: 1203 2328 5332 6309 1203
Size: 1125 3004 977 1327
Fragments arranged by size:
3004 1327 1125 977
AgeI A'CCGG_T
Cuts at: 5264 5264
Size: 6433
AhdI GACnn_n'nnGTC
Cuts at: 5162 5162
Size: 6433
AleI CACnn'nnGTG
Cuts at: 1166 3255 1166
Size: 2089 4344
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2166 1184
Size: 982 5451
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2584 2616 2584
Size: 32 6401
AlwNI CAG_nnn'CTG
Cuts at: 2383 4176 5748 2383
Size: 1793 1572 3068
Fragments arranged by size:
3068 1793 1572
ApaLI G'TGCA_C
Cuts at: 4490 5646 4490
Size: 1156 5277
ApoI r'AATT_y
Cuts at: 1493 3742 3968 4120 6229 1493
Size: 2249 226 152 2109 1697
Fragments arranged by size:
2249 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4987 160
Size: 872 3955 1606
Fragments arranged by size:
3955 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6433
AvaI C'yCGr_G
Cuts at: 1870 1943 3758 1870
Size: 73 1815 4545
Fragments arranged by size:
4545 1815 73
BamHI G'GATC_C
Cuts at: 3383 3763 4130 3383
Size: 380 367 5686
Fragments arranged by size:
5686 380 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3590 3754 5209 618
Size: 325 217 627 1803 164 1455 1842
Fragments arranged by size:
1842 1803 1455 627 325 217 164
BanII G_rGCy'C
Cuts at: 1391 2064 2139 2390 2499 3752 1391
Size: 673 75 251 109 1253 4072
Fragments arranged by size:
4072 1253 673 251 109 75
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2194 3581 3646 928
Size: 971 295 1387 65 3715
Fragments arranged by size:
3715 1387 971 295 65
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4624 4658 1903
Size: 34 2687 34 3678
Fragments arranged by size:
3678 2687 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3005 4325 5535 3005
Size: 1320 1210 3903
Fragments arranged by size:
3903 1320 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6433
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2933 3236 5044
Size: 107 122 71 631 605 1261 303 1808
Cuts at: 5044 136
Size: 1525
Fragments arranged by size:
1808 1525 1261 631 605 303 122 107
71
BglII A'GATC_T
Cuts at: 6428 6428
Size: 6433
BlpI GC'TnA_GC
Cuts at: 3850 3850
Size: 6433
Bme1580I G_kGCm'C
Cuts at: 1790 2746 4494 5650 1790
Size: 956 1748 1156 2573
Fragments arranged by size:
2573 1748 1156 956
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6433
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3459 4164 5122
Size: 416 53 83 399 24 2042 705 958
Cuts at: 5122 442
Size: 1753
Fragments arranged by size:
2042 1753 958 705 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6433
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2440 3002 2440
Size: 562 5871
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2703 2836 3041 5093 1239
Size: 1464 133 205 2052 2579
Fragments arranged by size:
2579 2052 1464 205 133
Bpu10I CC'TnA_GC
Cuts at: 3429 3429
Size: 6433
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3296 4558 5423 5721 3296
Size: 1262 865 298 4008
Fragments arranged by size:
4008 1262 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5096 882
Size: 617 3597 2219
Fragments arranged by size:
3597 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3730 4224 493
Size: 711 2526 494 2702
Fragments arranged by size:
2702 2526 711 494
BsaBI GATnn'nnATC
Cuts at: 6427 6427
Size: 6433
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2433 4240 4622 5257
Size: 53 83 186 936 900 1807 382 635
Cuts at: 5257 275
Size: 1451
Fragments arranged by size:
1807 1451 936 900 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4866 5264 5538 5685 6096 4866
Size: 398 274 147 411 5203
Fragments arranged by size:
5203 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6208
Fragments arranged by size:
6208 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2495 1186
Size: 1309 5124
BseYI C'CCAG_C
Cuts at: 5636 6132 5636
Size: 496 5937
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5860
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3883 4644 5672 1540
Size: 275 186 1882 761 1028 2301
Fragments arranged by size:
2301 1882 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2390 2499 3027 4494 4579 5650 2390
Size: 109 528 1467 85 1071 3173
Fragments arranged by size:
3173 1467 1071 528 109 85
BsmI GAATG_Cn'
Cuts at: 3944 4037 3944
Size: 93 6340
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2064 2139 2390 2499 2746 3027 3752
Size: 399 274 75 251 109 247 281 725
Cuts at: 3752 4494 4579 5650 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 725 399 281 274 251
247 109 85 75
BspEI T'CCGG_A
Cuts at: 6096 6096
Size: 6433
BspHI T'CATG_A
Cuts at: 3555 4322 6052 3555
Size: 767 1730 3936
Fragments arranged by size:
3936 1730 767
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3788 844
Size: 2944 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4320 1394
Size: 429 2497 3507
Fragments arranged by size:
3507 2497 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2119 2841 3372 4928 5102 65
Size: 1183 871 722 531 1556 174 1396
Fragments arranged by size:
1556 1396 1183 871 722 531 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5077 5264 1700
Size: 110 3267 187 2869
Fragments arranged by size:
3267 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2469 3669 96
Size: 2373 1200 2860
Fragments arranged by size:
2860 2373 1200
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6433
BssSI C'ACGA_G
Cuts at: 4493 5505 4493
Size: 1012 5421
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6433
BstBI TT'CG_AA
Cuts at: 2007 5237 2007
Size: 3230 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6433
BstXI CCAn_nnnn'nTGG
Cuts at: 3083 3586 3707 3083
Size: 503 121 5809
Fragments arranged by size:
5809 503 121
BstYI r'GATC_y
Cuts at: 1972 2689 2908 3383 3763 4130 4515 4532 5984
Size: 717 219 475 380 367 385 17 1452
Cuts at: 5984 6070 6082 6428 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 717 475 385 380 367 346
219 86 17 12
BstZ17I GTA'TAC
Cuts at: 2086 2086
Size: 6433
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5881
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3181 487
Size: 683 56 1955 3739
Fragments arranged by size:
3739 1955 683 56
BtsI GCAGTG_nn'
Cuts at: 2859 3949 4743 4763 2859
Size: 1090 794 20 4529
Fragments arranged by size:
4529 1090 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6433
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6398
DraI TTT'AAA
Cuts at: 2233 3737 2233
Size: 1504 4929
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5440 809
Size: 507 4124 1802
Fragments arranged by size:
4124 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2925 3880 4769
Size: 54 1425 50 275 18 1095 955 889
Cuts at: 4769 6398 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1095 955 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3880 1537
Size: 275 2068 4090
Fragments arranged by size:
4090 2068 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2836 4363 1353
Size: 1483 1527 3423
Fragments arranged by size:
3423 1527 1483
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5020 5394 5540 387
Size: 1064 138 3431 374 146 1280
Fragments arranged by size:
3431 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2388 2497 2388
Size: 109 6324
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2703 2836 2940 3041 4496 5093 5880
Size: 393 1071 133 104 101 1455 597 787
Cuts at: 5880 6148 1239
Size: 268 1524
Fragments arranged by size:
1524 1455 1071 787 597 393 268 133
104 101
EcoNI CCTnn'n_nnAGG
Cuts at: 2504 2504
Size: 6433
EcoO109I rG'GnC_Cy
Cuts at: 2797 2797
Size: 6433
EcoRI G'AATT_C
Cuts at: 3742 4120 3742
Size: 378 6055
EcoRV GAT'ATC
Cuts at: 2551 2551
Size: 6433
FspI TGC'GCA
Cuts at: 4939 4939
Size: 6433
HaeII r_GCGC'y
Cuts at: 1678 5580 1678
Size: 3902 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2701 2733 3528 3560 5074
Size: 32 135 32 1129 32 795 32 1514
Cuts at: 5074 5106 5148 5180 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1514 1129 795 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2965 3622 3777 4023 669
Size: 988 281 1027 657 155 246 3079
Fragments arranged by size:
3079 1027 988 657 281 246 155
HindIII A'AGCT_T
Cuts at: 748 2364 748
Size: 1616 4817
HpaI GTT'AAC
Cuts at: 4023 4023
Size: 6433
KpnI G_GTAC'C
Cuts at: 3758 3758
Size: 6433
MfeI C'AATT_G
Cuts at: 3362 4032 3362
Size: 670 5763
MluI A'CGCG_T
Cuts at: 6309 6309
Size: 6433
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5547 5731 1305
Size: 4242 184 2007
Fragments arranged by size:
4242 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2927 10
Size: 54 1425 343 1095 3516
Fragments arranged by size:
3516 1425 1095 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2666 2864 3081 3255 3584 4391 4750
Size: 648 1500 198 217 174 329 807 359
Cuts at: 4750 4909 518
Size: 159 2042
Fragments arranged by size:
2042 1500 807 648 359 329 217 198
174 159
MspA1I CmG'CkG
Cuts at: 1606 3068 3849 4526 5674 5919 1606
Size: 1462 781 677 1148 245 2120
Fragments arranged by size:
2120 1462 1148 781 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5881
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6433
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6433
NotI GC'GGCC_GC
Cuts at: 3880 3880
Size: 6433
NspI r_CATG'y
Cuts at: 1132 1229 2332 3791 5336 1132
Size: 97 1103 1459 1545 2229
Fragments arranged by size:
2229 1545 1459 1103 97
PasI CC'CwG_GG
Cuts at: 1348 2377 3691 1348
Size: 1029 1314 4090
Fragments arranged by size:
4090 1314 1029
PciI A'CATG_T
Cuts at: 2328 5332 2328
Size: 3004 3429
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6433
PfoI T'CCnGG_A
Cuts at: 2852 2852
Size: 6433
PmeI GTTT'AAAC
Cuts at: 3737 3737
Size: 6433
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4502 4534 6015 6047 4502
Size: 32 1481 32 4888
Fragments arranged by size:
4888 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2797 2797
Size: 6433
PshAI GACnn'nnGTC
Cuts at: 1613 2970 1613
Size: 1357 5076
PsiI TTA'TAA
Cuts at: 4003 4003
Size: 6433
PstI C_TGCA'G
Cuts at: 830 3785 830
Size: 2955 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6433
PvuII CAG'CTG
Cuts at: 3068 3068
Size: 6433
SacI G_AGCT'C
Cuts at: 2390 2499 2390
Size: 109 6324
SalI G'TCGA_C
Cuts at: 1936 2963 3775 1936
Size: 1027 812 4594
Fragments arranged by size:
4594 1027 812
SanDI GG'GwC_CC
Cuts at: 2797 2797
Size: 6433
SapI GCTCTTCn'nnn_
Cuts at: 2836 2836
Size: 6433
SbfI CC_TGCA'GG
Cuts at: 3785 3785
Size: 6433
ScaI AGT'ACT
Cuts at: 4681 6393 4681
Size: 1712 4721
SfcI C'TryA_G
Cuts at: 826 1045 3781 4916 5597 5788 826
Size: 219 2736 1135 681 191 1471
Fragments arranged by size:
2736 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6433
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6433
SmaI CCC'GGG
Cuts at: 1872 3760 1872
Size: 1888 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2411 3311 4537 5438 5700 820
Size: 197 926 468 900 1226 901 262 1553
Fragments arranged by size:
1553 1226 926 901 900 468 262 197
SnaBI TAC'GTA
Cuts at: 493 3730 4224 493
Size: 3237 494 2702
Fragments arranged by size:
3237 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6433
SphI G_CATG'C
Cuts at: 1132 3791 1132
Size: 2659 3774
SspI AAT'ATT
Cuts at: 5 52 4357 5
Size: 47 4305 2081
Fragments arranged by size:
4305 2081 47
StuI AGG'CCT
Cuts at: 1517 2054 1517
Size: 537 5896
StyI C'CwwG_G
Cuts at: 513 1065 2307 3872 513
Size: 552 1242 1565 3074
Fragments arranged by size:
3074 1565 1242 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3042 3089 3478 3578 3704 4460 4477
Size: 1255 1462 47 389 100 126 756 17
Cuts at: 4477 4630 4815 5260 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1462 1255 756 445 389 185 153
126 100 47 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2418 2469 3132
Size: 274 80 33 51 1443 441 51 663
Cuts at: 3132 3669 4679 6391 96
Size: 537 1010 1712 138
Fragments arranged by size:
1712 1443 1010 663 537 441 274 138
80 51 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6393 6425 6393
Size: 32 6401
Tth111I GACn'n_nGTC
Cuts at: 1316 3683 1316
Size: 2367 4066
XbaI T'CTAG_A
Cuts at: 3769 3769
Size: 6433
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2217 3601 2217
Size: 1384 5049
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6433
XmaI C'CCGG_G
Cuts at: 1870 3758 1870
Size: 1888 4545
XmnI GAAnn'nnTTC
Cuts at: 4562 4562
Size: 6433
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4241 276
Size: 53 83 186 936 2707 2468
Fragments arranged by size:
2707 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI Bsu36I DraIII FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspOMI PspXI PsrI RsrII SacII SexAI SfiI SfoI
SrfI SwaI