(Circular) (Six-base) MAPSORT of: pFN21ASDA1246.seq Check: 3570 from: 1 to: 7096 pFN21ASDA1246 7096 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3104 3104 Size: 7096 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4082 4906 278 Size: 53 83 186 936 2546 824 2468 Fragments arranged by size: 2546 2468 936 824 186 83 53 AccI GT'mk_AC Cuts at: 1937 2076 4389 4439 1937 Size: 139 2313 50 4594 Fragments arranged by size: 4594 2313 139 50 Acc65I G'GTAC_C Cuts at: 4417 4417 Size: 7096 AclI AA'CG_TT Cuts at: 1563 5223 5596 1563 Size: 3660 373 3063 Fragments arranged by size: 3660 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3330 5159 6543 6811 1632 Size: 1698 1829 1384 268 1917 Fragments arranged by size: 1917 1829 1698 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6899 AflIII A'CryG_T Cuts at: 1203 5995 6972 1203 Size: 4792 977 1327 Fragments arranged by size: 4792 1327 977 AgeI A'CCGG_T Cuts at: 5927 5927 Size: 7096 AhdI GACnn_n'nnGTC Cuts at: 2537 5825 2537 Size: 3288 3808 AleI CACnn'nnGTG Cuts at: 1166 2947 1166 Size: 1781 5315 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2372 4328 1184 Size: 1188 1956 3952 Fragments arranged by size: 3952 1956 1188 AlwNI CAG_nnn'CTG Cuts at: 2110 2617 2656 3476 3610 4839 6411 2110 Size: 507 39 820 134 1229 1572 2795 Fragments arranged by size: 2795 1572 1229 820 507 134 39 ApaI G_GGCC'C Cuts at: 3805 3818 3937 4125 4345 3805 Size: 13 119 188 220 6556 Fragments arranged by size: 6556 220 188 119 13 ApaLI G'TGCA_C Cuts at: 5153 6309 5153 Size: 1156 5940 ApoI r'AATT_y Cuts at: 1493 4405 4631 4783 6892 1493 Size: 2912 226 152 2109 1697 Fragments arranged by size: 2912 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5650 160 Size: 872 4618 1606 Fragments arranged by size: 4618 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7096 AvaI C'yCGr_G Cuts at: 1870 1943 2318 2789 3363 4086 4134 4344 4421 Size: 73 375 471 574 723 48 210 77 Cuts at: 4421 1870 Size: 4545 Fragments arranged by size: 4545 723 574 471 375 210 77 73 48 BamHI G'GATC_C Cuts at: 3945 4426 4793 3945 Size: 481 367 6248 Fragments arranged by size: 6248 481 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2594 3047 3086 3617 3764 Size: 325 217 627 807 453 39 531 147 Cuts at: 3764 4417 5872 618 Size: 653 1455 1842 Fragments arranged by size: 1842 1455 807 653 627 531 453 325 217 147 39 BanII G_rGCy'C Cuts at: 1391 3805 3818 3846 3937 4125 4345 4415 1391 Size: 2414 13 28 91 188 220 70 4072 Fragments arranged by size: 4072 2414 220 188 91 70 28 13 BbeI G_GCGC'C Cuts at: 3768 3768 Size: 7096 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3658 928 Size: 971 1759 4366 Fragments arranged by size: 4366 1759 971 BbvCI CC'TCA_GC Cuts at: 3167 3167 Size: 7096 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5287 5321 1903 Size: 34 3350 34 3678 Fragments arranged by size: 3678 3350 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4988 6198 4988 Size: 1210 5886 BclI T'GATC_A Cuts at: 1548 1548 Size: 7096 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2202 3122 3866 Size: 107 122 71 631 605 530 920 744 Cuts at: 3866 5707 136 Size: 1841 1525 Fragments arranged by size: 1841 1525 920 744 631 605 530 122 107 71 BglII A'GATC_T Cuts at: 7091 7091 Size: 7096 BlpI GC'TnA_GC Cuts at: 2555 4513 2555 Size: 1958 5138 Bme1580I G_kGCm'C Cuts at: 1790 2138 2597 3805 3818 3937 4125 4345 5157 Size: 348 459 1208 13 119 188 220 812 Cuts at: 5157 6313 1790 Size: 1156 2573 Fragments arranged by size: 2573 1208 1156 812 459 348 220 188 119 13 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7096 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2132 2995 3550 Size: 416 53 83 399 24 715 863 555 Cuts at: 3550 4827 5785 442 Size: 1277 958 1753 Fragments arranged by size: 1753 1277 958 863 715 555 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2051 1056 Size: 995 6101 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2945 3211 3877 3961 4033 4041 5756 1239 Size: 1706 266 666 84 72 8 1715 2579 Fragments arranged by size: 2579 1715 1706 666 266 84 72 8 Bpu10I CC'TnA_GC Cuts at: 3167 3167 Size: 7096 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2819 2930 3007 4001 5221 6086 6384 2819 Size: 111 77 994 1220 865 298 3531 Fragments arranged by size: 3531 1220 994 865 298 111 77 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2021 3148 4270 5759 882 Size: 617 522 1127 1122 1489 2219 Fragments arranged by size: 2219 1489 1127 1122 617 522 BsaAI yAC'GTr Cuts at: 493 1204 4393 4887 493 Size: 711 3189 494 2702 Fragments arranged by size: 3189 2702 711 494 BsaBI GATnn'nnATC Cuts at: 7090 7090 Size: 7096 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2079 3765 4079 4903 Size: 53 83 186 936 546 1686 314 824 Cuts at: 4903 5285 5920 275 Size: 382 635 1451 Fragments arranged by size: 1686 1451 936 824 635 546 382 314 186 83 53 BsaWI w'CCGG_w Cuts at: 5529 5927 6201 6348 6759 5529 Size: 398 274 147 411 5866 Fragments arranged by size: 5866 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2861 2891 4218 4248 1178 Size: 30 165 30 1458 30 1327 30 4026 Fragments arranged by size: 4026 1458 1327 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2879 2882 3010 3874 4049 4245 4337 1186 Size: 1693 3 128 864 175 196 92 3945 Fragments arranged by size: 3945 1693 864 196 175 128 92 3 BseYI C'CCAG_C Cuts at: 2428 2898 2969 3430 3716 3768 3925 3974 4073 Size: 470 71 461 286 52 157 49 99 Cuts at: 4073 4117 6299 6795 2428 Size: 44 2182 496 2729 Fragments arranged by size: 2729 2182 496 470 461 286 157 99 71 52 49 44 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3174 1163 Size: 573 1438 5085 Fragments arranged by size: 5085 1438 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3735 4546 5307 6335 1540 Size: 275 186 1734 811 761 1028 2301 Fragments arranged by size: 2301 1734 1028 811 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3846 4385 5157 5242 6313 3846 Size: 539 772 85 1071 4629 Fragments arranged by size: 4629 1071 772 539 85 BsmI GAATG_Cn' Cuts at: 4607 4700 4607 Size: 93 7003 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2138 2597 3805 3818 3846 3937 4125 Size: 399 348 459 1208 13 28 91 188 Cuts at: 4125 4345 4385 4415 5157 5242 6313 1391 Size: 220 40 30 742 85 1071 2174 Fragments arranged by size: 2174 1208 1071 742 459 399 348 220 188 91 85 40 30 28 13 BspEI T'CCGG_A Cuts at: 6759 6759 Size: 7096 BspHI T'CATG_A Cuts at: 4985 6715 4985 Size: 1730 5366 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2406 2580 2616 3104 4451 844 Size: 1562 174 36 488 1347 3489 Fragments arranged by size: 3489 1562 1347 488 174 36 BsrBI CCG'CTC Cuts at: 1394 1823 2331 2682 2794 4983 1394 Size: 429 508 351 112 2189 3507 Fragments arranged by size: 3507 2189 508 429 351 112 BsrDI GCAATG_nn' Cuts at: 65 1248 3099 3748 5591 5765 65 Size: 1183 1851 649 1843 174 1396 Fragments arranged by size: 1851 1843 1396 1183 649 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2175 5740 5927 1700 Size: 110 365 3565 187 2869 Fragments arranged by size: 3565 2869 365 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7096 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7096 BssSI C'ACGA_G Cuts at: 3707 5156 6168 3707 Size: 1449 1012 4635 Fragments arranged by size: 4635 1449 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2656 794 Size: 1862 5234 BstBI TT'CG_AA Cuts at: 2007 5900 2007 Size: 3893 3203 BstEII G'GTnAC_C Cuts at: 1184 2744 1184 Size: 1560 5536 BstXI CCAn_nnnn'nTGG Cuts at: 3133 3544 3628 3661 3724 3901 3133 Size: 411 84 33 63 177 6328 Fragments arranged by size: 6328 411 177 84 63 33 BstYI r'GATC_y Cuts at: 1972 2479 2629 3945 4426 4793 5178 5195 6647 Size: 507 150 1316 481 367 385 17 1452 Cuts at: 6647 6733 6745 7091 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1316 507 481 385 367 346 150 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2070 2506 2516 3126 3132 513 Size: 552 957 48 436 10 610 6 4477 Fragments arranged by size: 4477 957 610 552 436 48 10 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2422 2569 487 Size: 683 56 1196 147 5014 Fragments arranged by size: 5014 1196 683 147 56 BtsI GCAGTG_nn' Cuts at: 2755 3186 3262 3590 3913 4112 4612 5406 5426 Size: 431 76 328 323 199 500 794 20 Cuts at: 5426 2755 Size: 4425 Fragments arranged by size: 4425 794 500 431 328 323 199 76 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7096 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2695 2730 529 Size: 35 2131 35 4895 Fragments arranged by size: 4895 2131 35 35 DraI TTT'AAA Cuts at: 4400 4400 Size: 7096 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6103 809 Size: 507 4787 1802 Fragments arranged by size: 4787 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2937 3129 3498 Size: 54 1425 50 275 18 1107 192 369 Cuts at: 3498 3531 3651 3732 4543 5432 7061 8 Size: 33 120 81 811 889 1629 43 Fragments arranged by size: 1629 1425 1107 889 811 369 275 192 120 81 54 50 43 33 18 EagI C'GGCC_G Cuts at: 1537 1812 3732 4543 1537 Size: 275 1920 811 4090 Fragments arranged by size: 4090 1920 811 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3892 5026 1353 Size: 2539 1134 3423 Fragments arranged by size: 3423 2539 1134 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2194 3279 3946 5683 6057 6203 Size: 1064 138 605 1085 667 1737 374 146 Cuts at: 6203 387 Size: 1280 Fragments arranged by size: 1737 1280 1085 1064 667 605 374 146 138 EcoICRI GAG'CTC Cuts at: 3844 3844 Size: 7096 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2945 3211 3330 3877 3961 4033 4041 Size: 393 1313 266 119 547 84 72 8 Cuts at: 4041 5159 5756 6543 6811 1239 Size: 1118 597 787 268 1524 Fragments arranged by size: 1524 1313 1118 787 597 547 393 268 266 119 84 72 8 EcoNI CCTnn'n_nnAGG Cuts at: 2369 2369 Size: 7096 EcoO109I rG'GnC_Cy Cuts at: 2127 2386 2830 2965 3712 3801 3802 3814 3934 Size: 259 444 135 747 89 1 12 120 Cuts at: 3934 3996 4121 4122 4341 2127 Size: 62 125 1 219 4882 Fragments arranged by size: 4882 747 444 259 219 135 125 120 89 62 12 1 1 EcoRI G'AATT_C Cuts at: 4405 4783 4405 Size: 378 6718 FspI TGC'GCA Cuts at: 3836 5602 3836 Size: 1766 5330 HaeII r_GCGC'y Cuts at: 1678 3768 6243 1678 Size: 2090 2475 2531 Fragments arranged by size: 2531 2475 2090 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2523 2555 2621 2653 5737 Size: 32 135 32 951 32 66 32 3084 Cuts at: 5737 5769 5811 5843 1373 Size: 32 42 32 2626 Fragments arranged by size: 3084 2626 951 135 66 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2077 2395 2542 3457 4300 4440 Size: 988 281 139 318 147 915 843 140 Cuts at: 4440 4686 669 Size: 246 3079 Fragments arranged by size: 3079 988 915 843 318 281 246 147 140 139 HindIII A'AGCT_T Cuts at: 748 748 Size: 7096 HpaI GTT'AAC Cuts at: 4686 4686 Size: 7096 KasI G'GCGC_C Cuts at: 3764 3764 Size: 7096 KpnI G_GTAC'C Cuts at: 4421 4421 Size: 7096 MfeI C'AATT_G Cuts at: 2761 4695 2761 Size: 1934 5162 MluI A'CGCG_T Cuts at: 6972 6972 Size: 7096 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3462 4143 6210 6394 1305 Size: 2157 681 2067 184 2007 Fragments arranged by size: 2157 2067 2007 681 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3500 3653 10 Size: 54 1425 343 1668 153 3453 Fragments arranged by size: 3453 1668 1425 343 153 54 MslI CAynn'nnrTG Cuts at: 518 1166 2859 2947 3131 3647 3680 5054 5413 Size: 648 1693 88 184 516 33 1374 359 Cuts at: 5413 5572 518 Size: 159 2042 Fragments arranged by size: 2042 1693 1374 648 516 359 184 159 88 33 MspA1I CmG'CkG Cuts at: 1606 2428 2554 3032 3157 3382 3731 4512 5189 Size: 822 126 478 125 225 349 781 677 Cuts at: 5189 6337 6582 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 822 781 677 478 349 245 225 126 125 NarI GG'CG_CC Cuts at: 3765 3765 Size: 7096 NcoI C'CATG_G Cuts at: 513 1065 2022 2506 3132 513 Size: 552 957 484 626 4477 Fragments arranged by size: 4477 957 626 552 484 NdeI CA'TA_TG Cuts at: 387 387 Size: 7096 NheI G'CTAG_C Cuts at: 1052 2047 1052 Size: 995 6101 NotI GC'GGCC_GC Cuts at: 4543 4543 Size: 7096 NspI r_CATG'y Cuts at: 1132 1229 2858 4309 4454 5999 1132 Size: 97 1629 1451 145 1545 2229 Fragments arranged by size: 2229 1629 1545 1451 145 97 PasI CC'CwG_GG Cuts at: 1348 2825 3901 1348 Size: 1477 1076 4543 Fragments arranged by size: 4543 1477 1076 PciI A'CATG_T Cuts at: 5995 5995 Size: 7096 PflMI CCAn_nnn'nTGG Cuts at: 1108 2366 3610 1108 Size: 1258 1244 4594 Fragments arranged by size: 4594 1258 1244 PfoI T'CCnGG_A Cuts at: 2273 3846 4049 2273 Size: 1573 203 5320 Fragments arranged by size: 5320 1573 203 PmeI GTTT'AAAC Cuts at: 4400 4400 Size: 7096 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5165 5197 6678 6710 5165 Size: 32 1481 32 5551 Fragments arranged by size: 5551 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2127 2965 2127 Size: 838 6258 PshAI GACnn'nnGTC Cuts at: 1613 3032 1613 Size: 1419 5677 PsiI TTA'TAA Cuts at: 4666 4666 Size: 7096 PspOMI G'GGCC_C Cuts at: 3801 3814 3933 4121 4341 3801 Size: 13 119 188 220 6556 Fragments arranged by size: 6556 220 188 119 13 PspXI vC'TCGA_Gb Cuts at: 2789 2789 Size: 7096 PstI C_TGCA'G Cuts at: 830 2403 2613 2652 3387 4448 830 Size: 1573 210 39 735 1061 3478 Fragments arranged by size: 3478 1573 1061 735 210 39 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7096 PvuII CAG'CTG Cuts at: 2428 2554 3157 3382 2428 Size: 126 603 225 6142 Fragments arranged by size: 6142 603 225 126 RsrII CG'GwC_CG Cuts at: 4102 4102 Size: 7096 SacI G_AGCT'C Cuts at: 3846 3846 Size: 7096 SalI G'TCGA_C Cuts at: 1936 2075 4438 1936 Size: 139 2363 4594 Fragments arranged by size: 4594 2363 139 SanDI GG'GwC_CC Cuts at: 2127 2965 2127 Size: 838 6258 SbfI CC_TGCA'GG Cuts at: 4448 4448 Size: 7096 ScaI AGT'ACT Cuts at: 2093 5344 7056 2093 Size: 3251 1712 2133 Fragments arranged by size: 3251 2133 1712 SexAI A'CCwGG_T Cuts at: 3462 4332 3462 Size: 870 6226 SfcI C'TryA_G Cuts at: 826 1045 2399 2609 2648 3383 4246 4444 5579 Size: 219 1354 210 39 735 863 198 1135 Cuts at: 5579 6260 6451 826 Size: 681 191 1471 Fragments arranged by size: 1471 1354 1135 863 735 681 219 210 198 191 39 SfoI GGC'GCC Cuts at: 3766 3766 Size: 7096 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7096 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7096 SmaI CCC'GGG Cuts at: 1872 3365 4088 4136 4346 4423 1872 Size: 1493 723 48 210 77 4545 Fragments arranged by size: 4545 1493 723 210 77 48 SmlI C'TyrA_G Cuts at: 820 1017 1943 2318 2789 2834 2945 3022 4016 Size: 197 926 375 471 45 111 77 994 Cuts at: 4016 5200 6101 6363 820 Size: 1184 901 262 1553 Fragments arranged by size: 1553 1184 994 926 901 471 375 262 197 111 77 45 SnaBI TAC'GTA Cuts at: 493 4393 4887 493 Size: 3900 494 2702 Fragments arranged by size: 3900 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7096 SphI G_CATG'C Cuts at: 1132 4309 4454 1132 Size: 3177 145 3774 Fragments arranged by size: 3774 3177 145 SrfI GCCC'GGGC Cuts at: 4136 4136 Size: 7096 SspI AAT'ATT Cuts at: 5 52 5020 5 Size: 47 4968 2081 Fragments arranged by size: 4968 2081 47 StuI AGG'CCT Cuts at: 1517 3446 1517 Size: 1929 5167 StyI C'CwwG_G Cuts at: 513 1065 2022 2142 2365 2506 3132 3567 4535 Size: 552 957 120 223 141 626 435 968 Cuts at: 4535 513 Size: 3074 Fragments arranged by size: 3074 968 957 626 552 435 223 141 120 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2912 4119 4360 5123 5140 5293 5478 Size: 1255 1332 1207 241 763 17 153 185 Cuts at: 5478 5923 325 Size: 445 1498 Fragments arranged by size: 1498 1332 1255 1207 763 445 241 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2091 3387 3747 Size: 274 80 33 51 1443 114 1296 360 Cuts at: 3747 5342 7054 96 Size: 1595 1712 138 Fragments arranged by size: 1712 1595 1443 1296 360 274 138 114 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2861 2893 4218 4250 7056 7088 2861 Size: 32 1325 32 2806 32 2869 Fragments arranged by size: 2869 2806 1325 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2269 1316 Size: 953 6143 XbaI T'CTAG_A Cuts at: 4432 4432 Size: 7096 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3133 3328 3541 3133 Size: 195 213 6688 Fragments arranged by size: 6688 213 195 XhoI C'TCGA_G Cuts at: 1943 2318 2789 1943 Size: 375 471 6250 Fragments arranged by size: 6250 471 375 XmaI C'CCGG_G Cuts at: 1870 3363 4086 4134 4344 4421 1870 Size: 1493 723 48 210 77 4545 Fragments arranged by size: 4545 1493 723 210 77 48 XmnI GAAnn'nnTTC Cuts at: 5225 5225 Size: 7096 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4080 4904 276 Size: 53 83 186 936 2546 824 2468 Fragments arranged by size: 2546 2468 936 824 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PsrI SacII SapI SfiI SwaI