Restriction map for
pFN21ASDA1244
(Circular) (Six-base) MAPSORT of: pFN21ASDA1244.seq Check: 2755 from: 1 to: 10246
pFN21ASDA1244 10246 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3310 5998 3310
Size: 2688 7558
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4827 8056 278
Size: 53 83 186 936 3291 3229 2468
Fragments arranged by size:
3291 3229 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2679 2748 7589 1937
Size: 742 69 4841 4594
Fragments arranged by size:
4841 4594 742 69
Acc65I G'GTAC_C
Cuts at: 7567 7567
Size: 10246
AclI AA'CG_TT
Cuts at: 1563 8373 8746 1563
Size: 6810 373 3063
Fragments arranged by size:
6810 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2411 3017 3266 3720 4078 4460 5270 5714
Size: 779 606 249 454 358 382 810 444
Cuts at: 5714 5899 6398 6410 6965 7184 7231 8309 9693
Size: 185 499 12 555 219 47 1078 1384
Cuts at: 9693 9961 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1078 810 779 606 555 499
454 444 382 358 268 249 219 185
47 12
AfeI AGC'GCT
Cuts at: 4156 4156
Size: 10246
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 10049
AflIII A'CryG_T
Cuts at: 1203 2886 4045 4047 4678 9145 10122 1203
Size: 1683 1159 2 631 4467 977 1327
Fragments arranged by size:
4467 1683 1327 1159 977 631 2
AgeI A'CCGG_T
Cuts at: 2958 5351 9077 2958
Size: 2393 3726 4127
Fragments arranged by size:
4127 3726 2393
AhdI GACnn_n'nnGTC
Cuts at: 2899 8975 2899
Size: 6076 4170
AleI CACnn'nnGTG
Cuts at: 1166 3902 1166
Size: 2736 7510
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3132 1184
Size: 1948 8298
AlwNI CAG_nnn'CTG
Cuts at: 3046 3190 3928 4252 5199 5399 7989 9561 3046
Size: 144 738 324 947 200 2590 1572 3731
Fragments arranged by size:
3731 2590 1572 947 738 324 200 144
ApaI G_GGCC'C
Cuts at: 5798 5798
Size: 10246
ApaLI G'TGCA_C
Cuts at: 8303 9459 8303
Size: 1156 9090
ApoI r'AATT_y
Cuts at: 1493 7555 7781 7933 10042 1493
Size: 6062 226 152 2109 1697
Fragments arranged by size:
6062 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 8800 160
Size: 872 7768 1606
Fragments arranged by size:
7768 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10246
AvaI C'yCGr_G
Cuts at: 1870 1943 2555 2582 2872 2940 4175 4196 6371
Size: 73 612 27 290 68 1235 21 2175
Cuts at: 6371 6622 6915 7259 7571 1870
Size: 251 293 344 312 4545
Fragments arranged by size:
4545 2175 1235 612 344 312 293 290
251 73 68 27 21
AvrII C'CTAG_G
Cuts at: 2950 2950
Size: 10246
BamHI G'GATC_C
Cuts at: 7000 7576 7943 7000
Size: 576 367 9303
Fragments arranged by size:
9303 576 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2312 3289 4073 4103 6413
Size: 325 217 627 525 977 784 30 2310
Cuts at: 6413 7567 9022 618
Size: 1154 1455 1842
Fragments arranged by size:
2310 1842 1455 1154 977 784 627 525
325 217 30
BanII G_rGCy'C
Cuts at: 1391 2126 2328 3375 3671 4190 5155 5798 7310
Size: 735 202 1047 296 519 965 643 1512
Cuts at: 7310 7565 1391
Size: 255 4072
Fragments arranged by size:
4072 1512 1047 965 735 643 519 296
255 202
BbeI G_GCGC'C
Cuts at: 3293 6417 3293
Size: 3124 7122
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3340 5287 6594 928
Size: 971 1441 1947 1307 4580
Fragments arranged by size:
4580 1947 1441 1307 971
BbvCI CC'TCA_GC
Cuts at: 3176 3814 3998 4577 3176
Size: 638 184 579 8845
Fragments arranged by size:
8845 638 579 184
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3733 3767 6238 6272 8437 8471 1903
Size: 34 1796 34 2471 34 2165 34 3678
Fragments arranged by size:
3678 2471 2165 1796 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 8138 9348 8138
Size: 1210 9036
BclI T'GATC_A
Cuts at: 1548 2842 5679 1548
Size: 1294 2837 6115
Fragments arranged by size:
6115 2837 1294
BfrBI ATG'CAT
Cuts at: 2253 2253
Size: 10246
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3830 5726 6272
Size: 107 122 71 631 605 2158 1896 546
Cuts at: 6272 8857 136
Size: 2585 1525
Fragments arranged by size:
2585 2158 1896 1525 631 605 546 122
107 71
BglII A'GATC_T
Cuts at: 10241 10241
Size: 10246
BlpI GC'TnA_GC
Cuts at: 2327 2651 3779 6668 7663 2327
Size: 324 1128 2889 995 4910
Fragments arranged by size:
4910 2889 1128 995 324
Bme1580I G_kGCm'C
Cuts at: 1790 2317 4076 4915 5140 5798 5839 6655 8307
Size: 527 1759 839 225 658 41 816 1652
Cuts at: 8307 9463 1790
Size: 1156 2573
Fragments arranged by size:
2573 1759 1652 1156 839 816 658 527
225 41
BmgBI CAC'GTC
Cuts at: 1318 2887 3976 1318
Size: 1569 1089 7588
Fragments arranged by size:
7588 1569 1089
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3879 5111 5581
Size: 416 53 83 399 24 2462 1232 470
Cuts at: 5581 6022 7178 7977 8935 442
Size: 441 1156 799 958 1753
Fragments arranged by size:
2462 1753 1232 1156 958 799 470 441
416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10246
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2078 3804 2078
Size: 1726 8520
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2021 3950 4031 4100 4648 4980 5402 5734
Size: 782 1929 81 69 548 332 422 332
Cuts at: 5734 6167 6255 6794 7406 8906 1239
Size: 433 88 539 612 1500 2579
Fragments arranged by size:
2579 1929 1500 782 612 548 539 433
422 332 332 88 81 69
Bpu10I CC'TnA_GC
Cuts at: 3133 3176 3814 3998 4226 4577 6938 7291 3133
Size: 43 638 184 228 351 2361 353 6088
Fragments arranged by size:
6088 2361 638 353 351 228 184 43
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2365 2402 2600 4512 5849 8371 9236 9534 2365
Size: 37 198 1912 1337 2522 865 298 3077
Fragments arranged by size:
3077 2522 1912 1337 865 298 198 37
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4891 8909 882
Size: 617 3392 4018 2219
Fragments arranged by size:
4018 3392 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4048 4069 4615 7465 7543 8037 493
Size: 711 2844 21 546 2850 78 494 2702
Fragments arranged by size:
2850 2844 2702 711 546 494 78 21
BsaBI GATnn'nnATC
Cuts at: 10240 10240
Size: 10246
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3290 3735 4824 6414
Size: 53 83 186 936 1757 445 1089 1590
Cuts at: 6414 8053 8435 9070 275
Size: 1639 382 635 1451
Fragments arranged by size:
1757 1639 1590 1451 1089 936 635 445
382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2958 5351 8679 9077 9351 9498 9909 2958
Size: 2393 3328 398 274 147 411 3295
Fragments arranged by size:
3328 3295 2393 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3517 3547 1178
Size: 30 165 30 2114 30 7877
Fragments arranged by size:
7877 2114 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2207 2399 2518 3047 3104 3227 3383 5507
Size: 1021 192 119 529 57 123 156 2124
Cuts at: 5507 5825 5947 6803 1186
Size: 318 122 856 4629
Fragments arranged by size:
4629 2124 1021 856 529 318 192 156
123 122 119 57
BseYI C'CCAG_C
Cuts at: 2076 2153 2308 5140 5884 6003 6564 6977 7004
Size: 77 155 2832 744 119 561 413 27
Cuts at: 7004 7209 7504 9449 9945 2076
Size: 205 295 1945 496 2377
Fragments arranged by size:
2832 2377 1945 744 561 496 413 295
205 155 119 77 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2284 2717 3204 3344 3947 3968 6704
Size: 573 548 433 487 140 603 21 2736
Cuts at: 6704 1163
Size: 4705
Fragments arranged by size:
4705 2736 603 573 548 487 433 140
21
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3281 6510 7696 8457 9485 1540
Size: 275 186 1280 3229 1186 761 1028 2301
Fragments arranged by size:
3229 2301 1280 1186 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2037 2328 3375 3390 3671 4086 4203 4232 4353
Size: 291 1047 15 281 415 117 29 121
Cuts at: 4353 5637 6035 6924 7310 8307 8392 9463 2037
Size: 1284 398 889 386 997 85 1071 2820
Fragments arranged by size:
2820 1284 1071 1047 997 889 415 398
386 291 281 121 117 85 29 15
BsmI GAATG_Cn'
Cuts at: 4573 7036 7240 7757 7850 4573
Size: 2463 204 517 93 6969
Fragments arranged by size:
6969 2463 517 204 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2138 2639 7323 2138
Size: 501 4684 5061
Fragments arranged by size:
5061 4684 501
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2037 2126 2317 2328 3375 3390 3671
Size: 399 247 89 191 11 1047 15 281
Cuts at: 3671 4076 4086 4190 4203 4232 4353 4915 5140
Size: 405 10 104 13 29 121 562 225
Cuts at: 5140 5155 5637 5798 5839 6035 6655 6924 7310
Size: 15 482 161 41 196 620 269 386
Cuts at: 7310 7565 8307 8392 9463 1391
Size: 255 742 85 1071 2174
Fragments arranged by size:
2174 1071 1047 742 620 562 482 405
399 386 281 269 255 247 225 196
191 161 121 104 89 85 41 29
15 15 13 11 10
BspEI T'CCGG_A
Cuts at: 9909 9909
Size: 10246
BspHI T'CATG_A
Cuts at: 3312 4320 8135 9865 3312
Size: 1008 3815 1730 3693
Fragments arranged by size:
3815 3693 1730 1008
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2868 3310 5889 5998 6333 6972 7601 844
Size: 2024 442 2579 109 335 639 629 3489
Fragments arranged by size:
3489 2579 2024 639 629 442 335 109
BsrBI CCG'CTC
Cuts at: 1394 1823 3104 3730 4326 7290 8133 1394
Size: 429 1281 626 596 2964 843 3507
Fragments arranged by size:
3507 2964 1281 843 626 596 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4509 8741 8915 65
Size: 1183 3261 4232 174 1396
Fragments arranged by size:
4232 3261 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2958 5267 5351 6950 8890 9077 1700
Size: 110 1148 2309 84 1599 1940 187 2869
Fragments arranged by size:
2869 2309 1940 1599 1148 187 110 84
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 10246
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10246
BssSI C'ACGA_G
Cuts at: 8306 9318 8306
Size: 1012 9234
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2368 794
Size: 1574 8672
BstBI TT'CG_AA
Cuts at: 2007 9050 2007
Size: 7043 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 10246
BstXI CCAn_nnnn'nTGG
Cuts at: 3679 3679
Size: 10246
BstYI r'GATC_y
Cuts at: 1972 2212 2771 4881 7000 7344 7576 7943 8328
Size: 240 559 2110 2119 344 232 367 385
Cuts at: 8328 8345 9797 9883 9895 10241 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
2119 2110 1977 1452 559 385 367 346
344 240 232 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 5192 5192
Size: 10246
BtgI C'CryG_G
Cuts at: 513 1065 2421 2483 3256 4306 4977 5414 5997
Size: 552 1356 62 773 1050 671 437 583
Cuts at: 5997 7053 513
Size: 1056 3706
Fragments arranged by size:
3706 1356 1056 1050 773 671 583 552
437 62
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4113 4434 6455 487
Size: 683 56 2887 321 2021 4278
Fragments arranged by size:
4278 2887 2021 683 321 56
BtsI GCAGTG_nn'
Cuts at: 3223 3496 3966 5311 5511 6575 7437 7762 8556
Size: 273 470 1345 200 1064 862 325 794
Cuts at: 8556 8576 3223
Size: 20 4893
Fragments arranged by size:
4893 1345 1064 862 794 470 325 273
200 20
ClaI AT'CG_AT
Cuts at: 1554 3627 1554
Size: 2073 8173
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 10211
DraI TTT'AAA
Cuts at: 7550 7550
Size: 10246
DraIII CAC_nnn'GTG
Cuts at: 2056 2086 2484 4651 6030 2056
Size: 30 398 2167 1379 6272
Fragments arranged by size:
6272 2167 1379 398 30
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4838 9253 809
Size: 507 3522 4415 1802
Fragments arranged by size:
4415 3522 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3278 3502 4040
Size: 54 1425 50 275 18 1448 224 538
Cuts at: 4040 4338 5214 5727 6507 7693 8582 10211 8
Size: 298 876 513 780 1186 889 1629 43
Fragments arranged by size:
1629 1448 1425 1186 889 876 780 538
513 298 275 224 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3278 6507 7693 1537
Size: 275 1466 3229 1186 4090
Fragments arranged by size:
4090 3229 1466 1186 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2922 3858 4781 6204 6632 8176 1353
Size: 1569 936 923 1423 428 1544 3423
Fragments arranged by size:
3423 1569 1544 1423 936 923 428
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3026 4547 5534 8833 9207 9353
Size: 1064 138 1437 1521 987 3299 374 146
Cuts at: 9353 387
Size: 1280
Fragments arranged by size:
3299 1521 1437 1280 1064 987 374 146
138
EcoICRI GAG'CTC
Cuts at: 2326 3373 3669 7308 2326
Size: 1047 296 3639 5264
Fragments arranged by size:
5264 3639 1047 296
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2021 2411 3017 3266 3720 3950 4031
Size: 393 389 390 606 249 454 230 81
Cuts at: 4031 4078 4100 4460 4648 4980 5270 5402 5714
Size: 47 22 360 188 332 290 132 312
Cuts at: 5714 5734 5899 6167 6255 6398 6410 6794 6965
Size: 20 165 268 88 143 12 384 171
Cuts at: 6965 7184 7231 7406 8309 8906 9693 9961 1239
Size: 219 47 175 903 597 787 268 1524
Fragments arranged by size:
1524 903 787 606 597 454 393 390
389 384 360 332 312 290 268 268
249 230 219 188 175 171 165 143
132 88 81 47 47 22 20 12
EcoNI CCTnn'n_nnAGG
Cuts at: 4652 6533 4652
Size: 1881 8365
EcoO109I rG'GnC_Cy
Cuts at: 2150 2763 4171 4264 5698 5795 5878 6224 6526
Size: 613 1408 93 1434 97 83 346 302
Cuts at: 6526 6961 7172 7256 7284 2150
Size: 435 211 84 28 5112
Fragments arranged by size:
5112 1434 1408 613 435 346 302 211
97 93 84 83 28
EcoRI G'AATT_C
Cuts at: 7555 7933 7555
Size: 378 9868
EcoRV GAT'ATC
Cuts at: 5662 5662
Size: 10246
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2132 5719 6153 2132
Size: 3587 434 6225
Fragments arranged by size:
6225 3587 434
FspI TGC'GCA
Cuts at: 8752 8752
Size: 10246
HaeII r_GCGC'y
Cuts at: 1678 3027 3293 4158 4222 4545 5200 6417 9393
Size: 1349 266 865 64 323 655 1217 2976
Cuts at: 9393 1678
Size: 2531
Fragments arranged by size:
2976 2531 1349 1217 865 655 323 266
64
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2338 2370 2834 2866 2885
Size: 32 135 32 766 32 464 32 19
Cuts at: 2885 2917 3133 3165 3620 3652 4091 4123 5567
Size: 32 216 32 455 32 439 32 1444
Cuts at: 5567 5599 5837 5869 8887 8919 8961 8993 1373
Size: 32 238 32 3018 32 42 32 2626
Fragments arranged by size:
3018 2626 1444 766 464 455 439 238
216 135 42 32 32 32 32 32
32 32 32 32 32 32 32 19
HincII GTy'rAC
Cuts at: 669 1657 1938 7590 7836 669
Size: 988 281 5652 246 3079
Fragments arranged by size:
5652 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2271 5016 5644 748
Size: 1523 2745 628 5350
Fragments arranged by size:
5350 2745 1523 628
HpaI GTT'AAC
Cuts at: 7836 7836
Size: 10246
KasI G'GCGC_C
Cuts at: 3289 6413 3289
Size: 3124 7122
KpnI G_GTAC'C
Cuts at: 7571 7571
Size: 10246
MfeI C'AATT_G
Cuts at: 5407 7845 5407
Size: 2438 7808
MluI A'CGCG_T
Cuts at: 10122 10122
Size: 10246
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2883 2907 4323 4804 9360 9544 1305
Size: 1578 24 1416 481 4556 184 2007
Fragments arranged by size:
4556 2007 1578 1416 481 184 24
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3504 4042 5216 5729 10
Size: 54 1425 343 1672 538 1174 513 4527
Fragments arranged by size:
4527 1672 1425 1174 538 513 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3458 3743 3902 4764 6296 6758 7535
Size: 648 2292 285 159 862 1532 462 777
Cuts at: 7535 8204 8563 8722 518
Size: 669 359 159 2042
Fragments arranged by size:
2292 2042 1532 862 777 669 648 462
359 285 159 159
MspA1I CmG'CkG
Cuts at: 1606 2448 2455 3218 3333 3508 3836 4308 4438
Size: 842 7 763 115 175 328 472 130
Cuts at: 4438 4804 5389 5396 5792 5901 5919 7195 7453
Size: 366 585 7 396 109 18 1276 258
Cuts at: 7453 7662 8339 9487 9732 1606
Size: 209 677 1148 245 2120
Fragments arranged by size:
2120 1276 1148 842 763 677 585 472
396 366 328 258 245 209 175 130
115 109 18 7 7
NaeI GCC'GGC
Cuts at: 5269 5269
Size: 10246
NarI GG'CG_CC
Cuts at: 3290 6414 3290
Size: 3124 7122
NcoI C'CATG_G
Cuts at: 513 1065 2421 4977 5997 7053 513
Size: 552 1356 2556 1020 1056 3706
Fragments arranged by size:
3706 2556 1356 1056 1020 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 10246
NgoMIV G'CCGG_C
Cuts at: 5267 5267
Size: 10246
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10246
NotI GC'GGCC_GC
Cuts at: 7693 7693
Size: 10246
NsiI A_TGCA'T
Cuts at: 2255 2255
Size: 10246
NspI r_CATG'y
Cuts at: 1132 1229 2253 3403 4682 7604 9149 1132
Size: 97 1024 1150 1279 2922 1545 2229
Fragments arranged by size:
2922 2229 1545 1279 1150 1024 97
PasI CC'CwG_GG
Cuts at: 1348 7176 1348
Size: 5828 4418
PciI A'CATG_T
Cuts at: 4678 9145 4678
Size: 4467 5779
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3511 3650 5492 6469 1108
Size: 2403 139 1842 977 4885
Fragments arranged by size:
4885 2403 1842 977 139
PfoI T'CCnGG_A
Cuts at: 4260 4272 6232 4260
Size: 12 1960 8274
Fragments arranged by size:
8274 1960 12
PmeI GTTT'AAAC
Cuts at: 7550 7550
Size: 10246
PmlI CAC'GTG
Cuts at: 4048 4615 7465 4048
Size: 567 2850 6829
Fragments arranged by size:
6829 2850 567
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3104 3136 8315 8347 9828 9860 3104
Size: 32 5179 32 1481 32 3490
Fragments arranged by size:
5179 3490 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2150 2763 4171 4264 5698 5878 6526 6961 7172
Size: 613 1408 93 1434 180 648 435 211
Cuts at: 7172 2150
Size: 5224
Fragments arranged by size:
5224 1434 1408 648 613 435 211 180
93
PshAI GACnn'nnGTC
Cuts at: 1613 4681 4724 5352 1613
Size: 3068 43 628 6507
Fragments arranged by size:
6507 3068 628 43
PsiI TTA'TAA
Cuts at: 7816 7816
Size: 10246
PspOMI G'GGCC_C
Cuts at: 5794 5794
Size: 10246
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 7031 7063 7031
Size: 32 10214
PstI C_TGCA'G
Cuts at: 830 2756 3111 7598 830
Size: 1926 355 4487 3478
Fragments arranged by size:
4487 3478 1926 355
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10246
PvuII CAG'CTG
Cuts at: 3218 3508 3836 4438 4804 5389 7453 3218
Size: 290 328 602 366 585 2064 6011
Fragments arranged by size:
6011 2064 602 585 366 328 290
SacI G_AGCT'C
Cuts at: 2328 3375 3671 7310 2328
Size: 1047 296 3639 5264
Fragments arranged by size:
5264 3639 1047 296
SacII CC_GC'GG
Cuts at: 4309 4309
Size: 10246
SalI G'TCGA_C
Cuts at: 1936 7588 1936
Size: 5652 4594
SanDI GG'GwC_CC
Cuts at: 4171 7172 4171
Size: 3001 7245
SapI GCTCTTCn'nnn_
Cuts at: 2922 2922
Size: 10246
SbfI CC_TGCA'GG
Cuts at: 7598 7598
Size: 10246
ScaI AGT'ACT
Cuts at: 2849 5981 8494 10206 2849
Size: 3132 2513 1712 2889
Fragments arranged by size:
3132 2889 2513 1712
SexAI A'CCwGG_T
Cuts at: 4647 4833 4959 4647
Size: 186 126 9934
Fragments arranged by size:
9934 186 126
SfcI C'TryA_G
Cuts at: 826 1045 2752 3107 3268 3525 7594 8729 9410
Size: 219 1707 355 161 257 4069 1135 681
Cuts at: 9410 9601 826
Size: 191 1471
Fragments arranged by size:
4069 1707 1471 1135 681 355 257 219
191 161
SfoI GGC'GCC
Cuts at: 3291 6415 3291
Size: 3124 7122
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10246
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10246
SmaI CCC'GGG
Cuts at: 1872 2942 6373 6917 7573 1872
Size: 1070 3431 544 656 4545
Fragments arranged by size:
4545 3431 1070 656 544
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2344 2381 2555 2615 4527 5864
Size: 197 926 401 37 174 60 1912 1337
Cuts at: 5864 8350 9251 9513 820
Size: 2486 901 262 1553
Fragments arranged by size:
2486 1912 1553 1337 926 901 401 262
197 174 60 37
SnaBI TAC'GTA
Cuts at: 493 7543 8037 493
Size: 7050 494 2702
Fragments arranged by size:
7050 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10246
SphI G_CATG'C
Cuts at: 1132 2253 7604 1132
Size: 1121 5351 3774
Fragments arranged by size:
5351 3774 1121
SspI AAT'ATT
Cuts at: 5 52 5247 8170 5
Size: 47 5195 2923 2081
Fragments arranged by size:
5195 2923 2081 47
StuI AGG'CCT
Cuts at: 1517 3054 3359 4008 1517
Size: 1537 305 649 7755
Fragments arranged by size:
7755 1537 649 305
StyI C'CwwG_G
Cuts at: 513 1065 2421 2950 4165 4341 4416 4977 5997
Size: 552 1356 529 1215 176 75 561 1020
Cuts at: 5997 7053 7225 7685 513
Size: 1056 172 460 3074
Fragments arranged by size:
3074 1356 1215 1056 1020 561 552 529
460 176 172 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2258 2300 3482 4548 6041 6688 7103
Size: 1255 678 42 1182 1066 1493 647 415
Cuts at: 7103 7484 8273 8290 8443 8628 9073 325
Size: 381 789 17 153 185 445 1498
Fragments arranged by size:
1498 1493 1255 1182 1066 789 678 647
445 415 381 185 153 42 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2847 5979 8492
Size: 274 80 33 51 1443 870 3132 2513
Cuts at: 8492 10204 96
Size: 1712 138
Fragments arranged by size:
3132 2513 1712 1443 870 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4163 4195 6014 6046 6053 6085 7106 7138 10206
Size: 32 1819 32 7 32 1021 32 3068
Cuts at: 10206 10238 4163
Size: 32 4171
Fragments arranged by size:
4171 3068 1819 1021 32 32 32 32
32 7
Tth111I GACn'n_nGTC
Cuts at: 1316 2761 4268 1316
Size: 1445 1507 7294
Fragments arranged by size:
7294 1507 1445
XbaI T'CTAG_A
Cuts at: 7582 7582
Size: 10246
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4097 4145 4855 5752 6985 4097
Size: 48 710 897 1233 7358
Fragments arranged by size:
7358 1233 897 710 48
XhoI C'TCGA_G
Cuts at: 1943 2555 1943
Size: 612 9634
XmaI C'CCGG_G
Cuts at: 1870 2940 6371 6915 7571 1870
Size: 1070 3431 544 656 4545
Fragments arranged by size:
4545 3431 1070 656 544
XmnI GAAnn'nnTTC
Cuts at: 3308 5019 8375 3308
Size: 1711 3356 5179
Fragments arranged by size:
5179 3356 1711
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4825 8054 276
Size: 53 83 186 936 3291 3229 2468
Fragments arranged by size:
3291 3229 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BsiWI BstZ17I FseI FspAI NruI
PacI PspXI RsrII SfiI SrfI SwaI