Restriction map for
pFN21ASDA1242
(Circular) (Six-base) MAPSORT of: pFN21ASDA1242.seq Check: 4083 from: 1 to: 6208
pFN21ASDA1242 6208 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2651 2651
Size: 6208
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4018 278
Size: 53 83 186 936 2482 2468
Fragments arranged by size:
2482 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3551 1937
Size: 1614 4594
Acc65I G'GTAC_C
Cuts at: 3529 3529
Size: 6208
AclI AA'CG_TT
Cuts at: 1563 4335 4708 1563
Size: 2772 373 3063
Fragments arranged by size:
3063 2772 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4271 5655 5923 1632
Size: 2639 1384 268 1917
Fragments arranged by size:
2639 1917 1384 268
AfeI AGC'GCT
Cuts at: 2429 2429
Size: 6208
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6011
AflIII A'CryG_T
Cuts at: 1203 2089 2839 5107 6084 1203
Size: 886 750 2268 977 1327
Fragments arranged by size:
2268 1327 977 886 750
AgeI A'CCGG_T
Cuts at: 5039 5039
Size: 6208
AhdI GACnn_n'nnGTC
Cuts at: 2608 4937 2608
Size: 2329 3879
AleI CACnn'nnGTG
Cuts at: 1166 2645 3202 1166
Size: 1479 557 4172
Fragments arranged by size:
4172 1479 557
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2918 1184
Size: 1734 4474
AlwNI CAG_nnn'CTG
Cuts at: 2500 3951 5523 2500
Size: 1451 1572 3185
Fragments arranged by size:
3185 1572 1451
ApaLI G'TGCA_C
Cuts at: 4265 5421 4265
Size: 1156 5052
ApoI r'AATT_y
Cuts at: 1493 3517 3743 3895 6004 1493
Size: 2024 226 152 2109 1697
Fragments arranged by size:
2109 2024 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4762 160
Size: 872 3730 1606
Fragments arranged by size:
3730 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6208
AvaI C'yCGr_G
Cuts at: 1870 1943 2567 3010 3049 3139 3533 1870
Size: 73 624 443 39 90 394 4545
Fragments arranged by size:
4545 624 443 394 90 73 39
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3148 3181 3148
Size: 33 6175
BamHI G'GATC_C
Cuts at: 3495 3538 3905 3495
Size: 43 367 5798
Fragments arranged by size:
5798 367 43
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2043 2324 2639 3529 4984
Size: 325 217 627 256 281 315 890 1455
Cuts at: 4984 618
Size: 1842
Fragments arranged by size:
1842 1455 890 627 325 315 281 256
217
BanII G_rGCy'C
Cuts at: 1391 2979 3062 3221 3527 1391
Size: 1588 83 159 306 4072
Fragments arranged by size:
4072 1588 306 159 83
BbeI G_GCGC'C
Cuts at: 2047 2047
Size: 6208
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5237
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4399 4433 1903
Size: 34 2462 34 3678
Fragments arranged by size:
3678 2462 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3176 4100 5310 3176
Size: 924 1210 4074
Fragments arranged by size:
4074 1210 924
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6208
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3098 4819 136
Size: 107 122 71 631 605 1426 1721 1525
Fragments arranged by size:
1721 1525 1426 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2532 2784 6203 2532
Size: 252 3419 2537
Fragments arranged by size:
3419 2537 252
BlpI GC'TnA_GC
Cuts at: 2624 2865 3625 2624
Size: 241 760 5207
Fragments arranged by size:
5207 760 241
Bme1580I G_kGCm'C
Cuts at: 1790 2765 3348 4269 5425 1790
Size: 975 583 921 1156 2573
Fragments arranged by size:
2573 1156 975 921 583
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6208
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2126 2229 3939
Size: 416 53 83 399 24 709 103 1710
Cuts at: 3939 4897 442
Size: 958 1753
Fragments arranged by size:
1753 1710 958 709 416 399 103 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6208
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2158 2212 2416 3429 4868 1239
Size: 919 54 204 1013 1439 2579
Fragments arranged by size:
2579 1439 1013 919 204 54
Bpu10I CC'TnA_GC
Cuts at: 2417 3056 2417
Size: 639 5569
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2291 2963 4333 5198 5496 2291
Size: 672 1370 865 298 3003
Fragments arranged by size:
3003 1370 865 672 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2151 3410 4871 882
Size: 617 652 1259 1461 2219
Fragments arranged by size:
2219 1461 1259 652 617
BsaAI yAC'GTr
Cuts at: 493 1204 2409 3505 3999 493
Size: 711 1205 1096 494 2702
Fragments arranged by size:
2702 1205 1096 711 494
BsaBI GATnn'nnATC
Cuts at: 2063 6202 2063
Size: 4139 2069
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2044 2117 2259 4015
Size: 53 83 186 936 511 73 142 1756
Cuts at: 4015 4397 5032 275
Size: 382 635 1451
Fragments arranged by size:
1756 1451 936 635 511 382 186 142
83 73 53
BsaWI w'CCGG_w
Cuts at: 2244 2505 3498 4641 5039 5313 5460 5871 2244
Size: 261 993 1143 398 274 147 411 2581
Fragments arranged by size:
2581 1143 993 411 398 274 261 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2650 2680 3203 3233 3455
Size: 30 165 30 1247 30 523 30 222
Cuts at: 3455 3485 1178
Size: 30 3901
Fragments arranged by size:
3901 1247 523 222 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2993 1186
Size: 1807 4401
BseYI C'CCAG_C
Cuts at: 2144 2320 5411 5907 2144
Size: 176 3091 496 2445
Fragments arranged by size:
3091 2445 496 176
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2363 2801 3086 1163
Size: 573 627 438 285 4285
Fragments arranged by size:
4285 627 573 438 285
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2893 3093 3658 4419 5447 1540
Size: 275 186 892 200 565 761 1028 2301
Fragments arranged by size:
2301 1028 892 761 565 275 200 186
BsiHKAI G_wGCw'C
Cuts at: 2625 3084 3272 3480 4269 4354 5425 2625
Size: 459 188 208 789 85 1071 3408
Fragments arranged by size:
3408 1071 789 459 208 188 85
BsmI GAATG_Cn'
Cuts at: 2866 3719 3812 2866
Size: 853 93 5262
Fragments arranged by size:
5262 853 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3405 3405
Size: 6208
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2625 2765 2979 3062 3084 3221 3272
Size: 399 835 140 214 83 22 137 51
Cuts at: 3272 3348 3480 3527 4269 4354 5425 1391
Size: 76 132 47 742 85 1071 2174
Fragments arranged by size:
2174 1071 835 742 399 214 140 137
132 85 83 76 51 47 22
BspEI T'CCGG_A
Cuts at: 5871 5871
Size: 6208
BspHI T'CATG_A
Cuts at: 4097 5827 4097
Size: 1730 4478
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2651 3563 844
Size: 1807 912 3489
Fragments arranged by size:
3489 1807 912
BsrBI CCG'CTC
Cuts at: 1394 1823 2041 2740 2941 4095 1394
Size: 429 218 699 201 1154 3507
Fragments arranged by size:
3507 1154 699 429 218 201
BsrDI GCAATG_nn'
Cuts at: 65 1248 3378 4703 4877 65
Size: 1183 2130 1325 174 1396
Fragments arranged by size:
2130 1396 1325 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2327 2958 4852 5039 1700
Size: 110 517 631 1894 187 2869
Fragments arranged by size:
2869 1894 631 517 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6208
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6208
BssSI C'ACGA_G
Cuts at: 4268 5280 4268
Size: 1012 5196
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3130 794
Size: 2336 3872
BstBI TT'CG_AA
Cuts at: 2007 5012 2007
Size: 3005 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6208
BstXI CCAn_nnnn'nTGG
Cuts at: 3262 3262
Size: 6208
BstYI r'GATC_y
Cuts at: 1972 2532 2541 2784 3294 3495 3538 3905 4290
Size: 560 9 243 510 201 43 367 385
Cuts at: 4290 4307 5759 5845 5857 6203 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 560 510 385 367 346 243
201 86 43 17 12 9
Bsu36I CC'TnA_GG
Cuts at: 3422 3422
Size: 6208
BtgI C'CryG_G
Cuts at: 513 1065 2022 2724 513
Size: 552 957 702 3997
Fragments arranged by size:
3997 957 702 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3088 487
Size: 683 56 1862 3607
Fragments arranged by size:
3607 1862 683 56
BtsI GCAGTG_nn'
Cuts at: 2927 3197 3209 3724 4518 4538 2927
Size: 270 12 515 794 20 4597
Fragments arranged by size:
4597 794 515 270 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6208
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6173
DraI TTT'AAA
Cuts at: 3512 3512
Size: 6208
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3463 5215 809
Size: 507 2147 1752 1802
Fragments arranged by size:
2147 1802 1752 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3090 3330 3655
Size: 54 1425 50 275 18 1260 240 325
Cuts at: 3655 4544 6173 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1260 889 325 275 240 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3090 3655 1537
Size: 275 1278 565 4090
Fragments arranged by size:
4090 1278 565 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3491 4138 1353
Size: 2138 647 3423
Fragments arranged by size:
3423 2138 647
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2122 4795 5169 5315 387
Size: 1064 138 533 2673 374 146 1280
Fragments arranged by size:
2673 1280 1064 533 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2158 2212 2416 3429 4271 4868 5655
Size: 393 526 54 204 1013 842 597 787
Cuts at: 5655 5923 1239
Size: 268 1524
Fragments arranged by size:
1524 1013 842 787 597 526 393 268
204 54
EcoNI CCTnn'n_nnAGG
Cuts at: 3323 3323
Size: 6208
EcoO109I rG'GnC_Cy
Cuts at: 2347 2347
Size: 6208
EcoRI G'AATT_C
Cuts at: 3517 3895 3517
Size: 378 5830
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2563 2563
Size: 6208
FspI TGC'GCA
Cuts at: 4714 4714
Size: 6208
HaeII r_GCGC'y
Cuts at: 1678 2047 2431 2852 5355 1678
Size: 369 384 421 2503 2531
Fragments arranged by size:
2531 2503 421 384 369
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2249 2281 2594 2626 2858
Size: 32 135 32 677 32 313 32 232
Cuts at: 2858 2890 3203 3235 3455 3487 4849 4881 4923
Size: 32 313 32 220 32 1362 32 42
Cuts at: 4923 4955 1373
Size: 32 2626
Fragments arranged by size:
2626 1362 677 313 313 232 220 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3552 3798 669
Size: 988 281 1614 246 3079
Fragments arranged by size:
3079 1614 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6208
HpaI GTT'AAC
Cuts at: 3798 3798
Size: 6208
KasI G'GCGC_C
Cuts at: 2043 2043
Size: 6208
KpnI G_GTAC'C
Cuts at: 3533 3533
Size: 6208
MfeI C'AATT_G
Cuts at: 3807 3807
Size: 6208
MluI A'CGCG_T
Cuts at: 2089 2839 6084 2089
Size: 750 3245 2213
Fragments arranged by size:
3245 2213 750
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2802 2916 5322 5506 1305
Size: 1497 114 2406 184 2007
Fragments arranged by size:
2406 2007 1497 184 114
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3332 10
Size: 54 1425 343 1500 2886
Fragments arranged by size:
2886 1500 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2099 2645 3202 4166 4525 4684 518
Size: 648 933 546 557 964 359 159 2042
Fragments arranged by size:
2042 964 933 648 557 546 359 159
MspA1I CmG'CkG
Cuts at: 1606 2872 3155 3162 3624 4301 5449 5694 1606
Size: 1266 283 7 462 677 1148 245 2120
Fragments arranged by size:
2120 1266 1148 677 462 283 245 7
NaeI GCC'GGC
Cuts at: 2329 2329
Size: 6208
NarI GG'CG_CC
Cuts at: 2044 2044
Size: 6208
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4699
Fragments arranged by size:
4699 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6208
NgoMIV G'CCGG_C
Cuts at: 2327 2327
Size: 6208
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6208
NotI GC'GGCC_GC
Cuts at: 3655 3655
Size: 6208
NspI r_CATG'y
Cuts at: 1132 1229 3566 5111 1132
Size: 97 2337 1545 2229
Fragments arranged by size:
2337 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6208
PciI A'CATG_T
Cuts at: 5107 5107
Size: 6208
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3108 1108
Size: 2000 4208
PfoI T'CCnGG_A
Cuts at: 2879 2879
Size: 6208
PmeI GTTT'AAAC
Cuts at: 3512 3512
Size: 6208
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4277 4309 5790 5822 4277
Size: 32 1481 32 4663
Fragments arranged by size:
4663 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2347 2347
Size: 6208
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6208
PsiI TTA'TAA
Cuts at: 3778 3778
Size: 6208
PspXI vC'TCGA_Gb
Cuts at: 3049 3049
Size: 6208
PstI C_TGCA'G
Cuts at: 830 2376 2577 2907 2934 2952 3204 3560 830
Size: 1546 201 330 27 18 252 356 3478
Fragments arranged by size:
3478 1546 356 330 252 201 27 18
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6208
PvuII CAG'CTG
Cuts at: 2872 3155 2872
Size: 283 5925
SalI G'TCGA_C
Cuts at: 1936 3550 1936
Size: 1614 4594
SbfI CC_TGCA'GG
Cuts at: 3560 3560
Size: 6208
ScaI AGT'ACT
Cuts at: 4456 6168 4456
Size: 1712 4496
SexAI A'CCwGG_T
Cuts at: 2748 2748
Size: 6208
SfcI C'TryA_G
Cuts at: 826 1045 2372 2573 2903 2930 2948 3200 3556
Size: 219 1327 201 330 27 18 252 356
Cuts at: 3556 4691 5372 5563 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1327 1135 681 356 330 252 219
201 191 27 18
SfiI GGCCn_nnn'nGGCC
Cuts at: 3098 3098
Size: 6208
SfoI GGC'GCC
Cuts at: 2045 2045
Size: 6208
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6208
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6208
SmaI CCC'GGG
Cuts at: 1872 3535 1872
Size: 1663 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2306 2978 3049 4312 5213 5475
Size: 197 926 363 672 71 1263 901 262
Cuts at: 5475 820
Size: 1553
Fragments arranged by size:
1553 1263 926 901 672 363 262 197
71
SnaBI TAC'GTA
Cuts at: 493 3505 3999 493
Size: 3012 494 2702
Fragments arranged by size:
3012 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6208
SphI G_CATG'C
Cuts at: 1132 3566 1132
Size: 2434 3774
SspI AAT'ATT
Cuts at: 5 52 4132 5
Size: 47 4080 2081
Fragments arranged by size:
4080 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6208
StyI C'CwwG_G
Cuts at: 513 1065 2022 2253 3647 513
Size: 552 957 231 1394 3074
Fragments arranged by size:
3074 1394 957 552 231
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2312 4235 4252 4405 4590 5035 325
Size: 1255 732 1923 17 153 185 445 1498
Fragments arranged by size:
1923 1498 1255 732 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3072 4454 6166
Size: 274 80 33 51 1443 1095 1382 1712
Cuts at: 6166 96
Size: 138
Fragments arranged by size:
1712 1443 1382 1095 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6168 6200 6168
Size: 32 6176
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6208
XbaI T'CTAG_A
Cuts at: 3544 3544
Size: 6208
XhoI C'TCGA_G
Cuts at: 1943 3049 1943
Size: 1106 5102
XmaI C'CCGG_G
Cuts at: 1870 3533 1870
Size: 1663 4545
XmnI GAAnn'nnTTC
Cuts at: 4337 4337
Size: 6208
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4016 276
Size: 53 83 186 936 2482 2468
Fragments arranged by size:
2482 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspXI PstI PvuI PvuII SalI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BbvCI BfrBI BplI BsiWI
BstZ17I DraIII EcoICRI EcoRV FseI FspAI NruI NsiI
PacI PmlI PspOMI PsrI RsrII SacI SacII SanDI
SapI SrfI SwaI XcmI