Restriction map for
pFN21ASDA1234
(Circular) (Six-base) MAPSORT of: pFN21ASDA1234.seq Check: 874 from: 1 to: 6886
pFN21ASDA1234 6886 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4031 4031
Size: 6886
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4696 278
Size: 53 83 186 936 3160 2468
Fragments arranged by size:
3160 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4229 1937
Size: 2292 4594
Acc65I G'GTAC_C
Cuts at: 4207 4207
Size: 6886
AclI AA'CG_TT
Cuts at: 1563 5013 5386 1563
Size: 3450 373 3063
Fragments arranged by size:
3450 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4949 6333 6601 1632
Size: 3317 1384 268 1917
Fragments arranged by size:
3317 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6689
AflIII A'CryG_T
Cuts at: 1203 5785 6762 1203
Size: 4582 977 1327
Fragments arranged by size:
4582 1327 977
AgeI A'CCGG_T
Cuts at: 5717 5717
Size: 6886
AhdI GACnn_n'nnGTC
Cuts at: 5615 5615
Size: 6886
AleI CACnn'nnGTG
Cuts at: 1166 4078 1166
Size: 2912 3974
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3506 4190 1184
Size: 2322 684 3880
Fragments arranged by size:
3880 2322 684
AlwNI CAG_nnn'CTG
Cuts at: 2354 4629 6201 2354
Size: 2275 1572 3039
Fragments arranged by size:
3039 2275 1572
ApaI G_GGCC'C
Cuts at: 2641 3142 2641
Size: 501 6385
ApaLI G'TGCA_C
Cuts at: 2053 3515 3993 4165 4943 6099 2053
Size: 1462 478 172 778 1156 2840
Fragments arranged by size:
2840 1462 1156 778 478 172
ApoI r'AATT_y
Cuts at: 1493 2822 4195 4421 4573 6682 1493
Size: 1329 1373 226 152 2109 1697
Fragments arranged by size:
2109 1697 1373 1329 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5440 160
Size: 872 4408 1606
Fragments arranged by size:
4408 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6886
AvaI C'yCGr_G
Cuts at: 1870 1943 2731 2745 2783 4109 4211 1870
Size: 73 788 14 38 1326 102 4545
Fragments arranged by size:
4545 1326 788 102 73 38 14
BamHI G'GATC_C
Cuts at: 4216 4583 4216
Size: 367 6519
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2315 2848 2899 3320 4105
Size: 325 217 627 528 533 51 421 785
Cuts at: 4105 4207 5662 618
Size: 102 1455 1842
Fragments arranged by size:
1842 1455 785 627 533 528 421 325
217 102 51
BanII G_rGCy'C
Cuts at: 1391 2297 2641 3142 3197 3452 3580 3871 3951
Size: 906 344 501 55 255 128 291 80
Cuts at: 3951 4065 4205 1391
Size: 114 140 4072
Fragments arranged by size:
4072 906 501 344 291 255 140 128
114 80 55
BbeI G_GCGC'C
Cuts at: 2319 2852 2319
Size: 533 6353
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5915
BbvCI CC'TCA_GC
Cuts at: 3567 4035 3567
Size: 468 6418
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3652 3686 5077 5111 1903
Size: 34 1715 34 1391 34 3678
Fragments arranged by size:
3678 1715 1391 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4778 5988 4778
Size: 1210 5676
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6886
BfrBI ATG'CAT
Cuts at: 2438 2438
Size: 6886
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2111 5497 136
Size: 107 122 71 631 605 439 3386 1525
Fragments arranged by size:
3386 1525 631 605 439 122 107 71
BglII A'GATC_T
Cuts at: 6881 6881
Size: 6886
BlpI GC'TnA_GC
Cuts at: 3230 3579 3870 4303 3230
Size: 349 291 433 5813
Fragments arranged by size:
5813 433 349 291
Bme1580I G_kGCm'C
Cuts at: 1790 2057 2641 2654 3124 3142 3416 3519 3738
Size: 267 584 13 470 18 274 103 219
Cuts at: 3738 3855 3997 4110 4169 4947 6103 1790
Size: 117 142 113 59 778 1156 2573
Fragments arranged by size:
2573 1156 778 584 470 274 267 219
142 117 113 103 59 18 13
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6886
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2560 3453 3961
Size: 416 53 83 399 24 1143 893 508
Cuts at: 3961 4072 4617 5575 442
Size: 111 545 958 1753
Fragments arranged by size:
1753 1143 958 893 545 508 416 399
111 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6886
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2311 2882 3282 3718 3835 2311
Size: 571 400 436 117 5362
Fragments arranged by size:
5362 571 436 400 117
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2384 3252 3264 3454 3594 3965 5546 1239
Size: 1145 868 12 190 140 371 1581 2579
Fragments arranged by size:
2579 1581 1145 868 371 190 140 12
Bpu10I CC'TnA_GC
Cuts at: 2633 3092 3196 3567 4035 2633
Size: 459 104 371 468 5484
Fragments arranged by size:
5484 468 459 371 104
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5011 5876 6174 5011
Size: 865 298 5723
Fragments arranged by size:
5723 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5549 882
Size: 617 4050 2219
Fragments arranged by size:
4050 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2058 2515 4183 4677 493
Size: 711 854 457 1668 494 2702
Fragments arranged by size:
2702 1668 854 711 494 457
BsaBI GATnn'nnATC
Cuts at: 6880 6880
Size: 6886
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2316 2849 4693 5075
Size: 53 83 186 936 783 533 1844 382
Cuts at: 5075 5710 275
Size: 635 1451
Fragments arranged by size:
1844 1451 936 783 635 533 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5319 5717 5991 6138 6549 5319
Size: 398 274 147 411 5656
Fragments arranged by size:
5656 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2042 2072 2192 2222 2892
Size: 30 165 30 639 30 120 30 670
Cuts at: 2892 2922 3344 3374 1178
Size: 30 422 30 4690
Fragments arranged by size:
4690 670 639 422 165 120 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2092 2900 2953 3224 3227 3559 1186
Size: 906 808 53 271 3 332 4513
Fragments arranged by size:
4513 906 808 332 271 53 3
BseYI C'CCAG_C
Cuts at: 3341 3737 3751 3775 3893 6089 6585 3341
Size: 396 14 24 118 2196 496 3642
Fragments arranged by size:
3642 2196 496 396 118 24 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2303 2436 3912 4003 1163
Size: 573 567 133 1476 91 4046
Fragments arranged by size:
4046 1476 573 567 133 91
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2313 2451 2524 4336 5097 6125
Size: 275 186 312 138 73 1812 761 1028
Cuts at: 6125 1540
Size: 2301
Fragments arranged by size:
2301 1812 1028 761 312 275 186 138
73
BsiHKAI G_wGCw'C
Cuts at: 2057 2297 3093 3519 3580 3997 4169 4947 5032
Size: 240 796 426 61 417 172 778 85
Cuts at: 5032 6103 2057
Size: 1071 2840
Fragments arranged by size:
2840 1071 796 778 426 417 240 172
85 61
BsiWI C'GTAC_G
Cuts at: 2058 2058
Size: 6886
BsmI GAATG_Cn'
Cuts at: 4397 4490 4397
Size: 93 6793
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2057 2297 2641 2654 3093 3124 3142
Size: 399 267 240 344 13 439 31 18
Cuts at: 3142 3197 3416 3452 3519 3580 3738 3855 3871
Size: 55 219 36 67 61 158 117 16
Cuts at: 3871 3951 3997 4065 4110 4169 4205 4947 5032
Size: 80 46 68 45 59 36 742 85
Cuts at: 5032 6103 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 439 399 344 267 240
219 158 117 85 80 68 67 61
59 55 46 45 36 36 31 18
16 13
BspEI T'CCGG_A
Cuts at: 6549 6549
Size: 6886
BspHI T'CATG_A
Cuts at: 2793 4775 6505 2793
Size: 1982 1730 3174
Fragments arranged by size:
3174 1982 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4031 4241 844
Size: 3187 210 3489
Fragments arranged by size:
3489 3187 210
BsrBI CCG'CTC
Cuts at: 1394 1823 3078 4773 1394
Size: 429 1255 1695 3507
Fragments arranged by size:
3507 1695 1255 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2892 5381 5555 65
Size: 1183 1644 2489 174 1396
Fragments arranged by size:
2489 1644 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2312 2538 5530 5717 1700
Size: 110 502 226 2992 187 2869
Fragments arranged by size:
2992 2869 502 226 187 110
BsrGI T'GTAC_A
Cuts at: 96 3834 96
Size: 3738 3148
BssHII G'CGCG_C
Cuts at: 1925 2943 1925
Size: 1018 5868
BssSI C'ACGA_G
Cuts at: 3592 4946 5958 3592
Size: 1354 1012 4520
Fragments arranged by size:
4520 1354 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2354 3226 3859 3935 794
Size: 1560 872 633 76 3745
Fragments arranged by size:
3745 1560 872 633 76
BstBI TT'CG_AA
Cuts at: 2007 5690 2007
Size: 3683 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6886
BstXI CCAn_nnnn'nTGG
Cuts at: 3691 3691
Size: 6886
BstYI r'GATC_y
Cuts at: 1972 4216 4583 4968 4985 6437 6523 6535 6881
Size: 2244 367 385 17 1452 86 12 346
Cuts at: 6881 1972
Size: 1977
Fragments arranged by size:
2244 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2951 2951
Size: 6886
BtgI C'CryG_G
Cuts at: 513 1065 2106 2556 2873 2985 3379 3606 3877
Size: 552 1041 450 317 112 394 227 271
Cuts at: 3877 513
Size: 3522
Fragments arranged by size:
3522 1041 552 450 394 317 271 227
112
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6147
Fragments arranged by size:
6147 683 56
BtsI GCAGTG_nn'
Cuts at: 3888 4402 5196 5216 3888
Size: 514 794 20 5558
Fragments arranged by size:
5558 794 514 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6886
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2433 2468 529
Size: 35 1869 35 4947
Fragments arranged by size:
4947 1869 35 35
DraI TTT'AAA
Cuts at: 4190 4190
Size: 6886
DraIII CAC_nnn'GTG
Cuts at: 3859 3935 3859
Size: 76 6810
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5893 809
Size: 507 4577 1802
Fragments arranged by size:
4577 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2175 2310 3112
Size: 54 1425 50 275 18 345 135 802
Cuts at: 3112 4134 4333 5222 6851 8
Size: 1022 199 889 1629 43
Fragments arranged by size:
1629 1425 1022 889 802 345 275 199
135 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2310 4333 1537
Size: 275 498 2023 4090
Fragments arranged by size:
4090 2023 498 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2258 4816 1353
Size: 905 2558 3423
Fragments arranged by size:
3423 2558 905
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3271 5473 5847 5993 387
Size: 1064 138 1682 2202 374 146 1280
Fragments arranged by size:
2202 1682 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2295 3578 2295
Size: 1283 5603
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2384 3252 3264 3454 3594 3965 4949
Size: 393 752 868 12 190 140 371 984
Cuts at: 4949 5546 6333 6601 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 984 868 787 752 597 393 371
268 190 140 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2346 2346
Size: 6886
EcoO109I rG'GnC_Cy
Cuts at: 2082 2587 2637 3138 3139 3237 3272 3649 2082
Size: 505 50 501 1 98 35 377 5319
Fragments arranged by size:
5319 505 501 377 98 50 35 1
EcoRI G'AATT_C
Cuts at: 4195 4573 4195
Size: 378 6508
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2287 2287
Size: 6886
FspI TGC'GCA
Cuts at: 5392 5392
Size: 6886
HaeII r_GCGC'y
Cuts at: 1678 2319 2852 6033 1678
Size: 641 533 3181 2531
Fragments arranged by size:
3181 2531 641 533
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2192 2224 2714 2746 3344
Size: 32 135 32 620 32 490 32 598
Cuts at: 3344 3376 3586 3618 5527 5559 5601 5633 1373
Size: 32 210 32 1909 32 42 32 2626
Fragments arranged by size:
2626 1909 620 598 490 210 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4230 4476 669
Size: 988 281 2292 246 3079
Fragments arranged by size:
3079 2292 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2222 4155 748
Size: 1474 1933 3479
Fragments arranged by size:
3479 1933 1474
HpaI GTT'AAC
Cuts at: 4476 4476
Size: 6886
KasI G'GCGC_C
Cuts at: 2315 2848 2315
Size: 533 6353
KpnI G_GTAC'C
Cuts at: 4211 4211
Size: 6886
MfeI C'AATT_G
Cuts at: 4485 4485
Size: 6886
MluI A'CGCG_T
Cuts at: 6762 6762
Size: 6886
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2145 2148 2361 3686 3951 6000 6184 1305
Size: 840 3 213 1325 265 2049 184 2007
Fragments arranged by size:
2049 2007 1325 840 265 213 184 3
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2177 4136 10
Size: 54 1425 343 345 1959 2760
Fragments arranged by size:
2760 1959 1425 345 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2513 2798 3148 3689 4078 4844 5203
Size: 648 1347 285 350 541 389 766 359
Cuts at: 5203 5362 518
Size: 159 2042
Fragments arranged by size:
2042 1347 766 648 541 389 359 350
285 159
MspA1I CmG'CkG
Cuts at: 1606 2337 2691 2767 2875 2924 2987 3299 3381
Size: 731 354 76 108 49 63 312 82
Cuts at: 3381 4302 4979 6127 6372 1606
Size: 921 677 1148 245 2120
Fragments arranged by size:
2120 1148 921 731 677 354 312 245
108 82 76 63 49
NaeI GCC'GGC
Cuts at: 2314 2540 2314
Size: 226 6660
NarI GG'CG_CC
Cuts at: 2316 2849 2316
Size: 533 6353
NcoI C'CATG_G
Cuts at: 513 1065 2556 3606 3877 513
Size: 552 1491 1050 271 3522
Fragments arranged by size:
3522 1491 1050 552 271
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6886
NgoMIV G'CCGG_C
Cuts at: 2312 2538 2312
Size: 226 6660
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6886
NotI GC'GGCC_GC
Cuts at: 4333 4333
Size: 6886
NsiI A_TGCA'T
Cuts at: 2440 2440
Size: 6886
NspI r_CATG'y
Cuts at: 1132 1229 4244 5789 1132
Size: 97 3015 1545 2229
Fragments arranged by size:
3015 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6886
PciI A'CATG_T
Cuts at: 5785 5785
Size: 6886
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4102 1108
Size: 2994 3892
PfoI T'CCnGG_A
Cuts at: 3268 3268
Size: 6886
PmeI GTTT'AAAC
Cuts at: 4190 4190
Size: 6886
PmlI CAC'GTG
Cuts at: 2515 2515
Size: 6886
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4955 4987 6468 6500 4955
Size: 32 1481 32 5341
Fragments arranged by size:
5341 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3272 3649 3272
Size: 377 6509
PshAI GACnn'nnGTC
Cuts at: 1613 2337 1613
Size: 724 6162
PsiI TTA'TAA
Cuts at: 4456 4456
Size: 6886
PspOMI G'GGCC_C
Cuts at: 2637 3138 2637
Size: 501 6385
PstI C_TGCA'G
Cuts at: 830 2091 3339 3555 3724 4028 4238 830
Size: 1261 1248 216 169 304 210 3478
Fragments arranged by size:
3478 1261 1248 304 216 210 169
PvuI CG_AT'CG
Cuts at: 2001 2451 2001
Size: 450 6436
PvuII CAG'CTG
Cuts at: 2337 2767 2337
Size: 430 6456
SacI G_AGCT'C
Cuts at: 2297 3580 2297
Size: 1283 5603
SacII CC_GC'GG
Cuts at: 2876 2988 3382 2876
Size: 112 394 6380
Fragments arranged by size:
6380 394 112
SalI G'TCGA_C
Cuts at: 1936 4228 1936
Size: 2292 4594
SapI GCTCTTCn'nnn_
Cuts at: 2258 2258
Size: 6886
SbfI CC_TGCA'GG
Cuts at: 4238 4238
Size: 6886
ScaI AGT'ACT
Cuts at: 2672 5134 6846 2672
Size: 2462 1712 2712
Fragments arranged by size:
2712 2462 1712
SexAI A'CCwGG_T
Cuts at: 3510 3510
Size: 6886
SfcI C'TryA_G
Cuts at: 826 1045 2087 3335 3551 3720 4024 4234 5369
Size: 219 1042 1248 216 169 304 210 1135
Cuts at: 5369 6050 6241 826
Size: 681 191 1471
Fragments arranged by size:
1471 1248 1135 1042 681 304 219 216
210 191 169
SfoI GGC'GCC
Cuts at: 2317 2850 2317
Size: 533 6353
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6886
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6886
SmaI CCC'GGG
Cuts at: 1872 2747 4111 4213 1872
Size: 875 1364 102 4545
Fragments arranged by size:
4545 1364 875 102
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4990 5891 6153 820
Size: 197 926 3047 901 262 1553
Fragments arranged by size:
3047 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4183 4677 493
Size: 3690 494 2702
Fragments arranged by size:
3690 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6886
SphI G_CATG'C
Cuts at: 1132 4244 1132
Size: 3112 3774
SspI AAT'ATT
Cuts at: 5 52 4810 5
Size: 47 4758 2081
Fragments arranged by size:
4758 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6886
StyI C'CwwG_G
Cuts at: 513 1065 2556 2595 3186 3606 3877 4065 4131
Size: 552 1491 39 591 420 271 188 66
Cuts at: 4131 4325 513
Size: 194 3074
Fragments arranged by size:
3074 1491 591 552 420 271 194 188
66 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3696 3789 4913 4930 5083 5268 5713
Size: 1255 2116 93 1124 17 153 185 445
Cuts at: 5713 325
Size: 1498
Fragments arranged by size:
2116 1498 1255 1124 445 185 153 93
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2670 3834 5132
Size: 274 80 33 51 1443 693 1164 1298
Cuts at: 5132 6844 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1298 1164 693 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2892 2924 3692 3724 6846 6878 2892
Size: 32 768 32 3122 32 2900
Fragments arranged by size:
3122 2900 768 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3173 1316
Size: 1857 5029
XbaI T'CTAG_A
Cuts at: 4222 4222
Size: 6886
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2576 4129 2576
Size: 1553 5333
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6886
XmaI C'CCGG_G
Cuts at: 1870 2745 4109 4211 1870
Size: 875 1364 102 4545
Fragments arranged by size:
4545 1364 875 102
XmnI GAAnn'nnTTC
Cuts at: 2267 5015 2267
Size: 2748 4138
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4694 276
Size: 53 83 186 936 3160 2468
Fragments arranged by size:
3160 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BsmBI BstZ17I EcoRV
FseI FspAI NruI PacI PspXI PsrI RsrII SanDI
SfiI SrfI SwaI