Restriction map for
pFN21ASDA1218
(Circular) (Six-base) MAPSORT of: pFN21ASDA1218.seq Check: 6079 from: 1 to: 7228
pFN21ASDA1218 7228 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2885 2885
Size: 7228
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5038 278
Size: 53 83 186 936 3502 2468
Fragments arranged by size:
3502 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2317 4571 1937
Size: 380 2254 4594
Fragments arranged by size:
4594 2254 380
Acc65I G'GTAC_C
Cuts at: 4549 4549
Size: 7228
AclI AA'CG_TT
Cuts at: 1563 5355 5728 1563
Size: 3792 373 3063
Fragments arranged by size:
3792 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3051 3555 5291 6675 6943 1632
Size: 1419 504 1736 1384 268 1917
Fragments arranged by size:
1917 1736 1419 1384 504 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7031
AflIII A'CryG_T
Cuts at: 1203 6127 7104 1203
Size: 4924 977 1327
Fragments arranged by size:
4924 1327 977
AgeI A'CCGG_T
Cuts at: 3217 4372 6059 3217
Size: 1155 1687 4386
Fragments arranged by size:
4386 1687 1155
AhdI GACnn_n'nnGTC
Cuts at: 5957 5957
Size: 7228
AleI CACnn'nnGTG
Cuts at: 1166 3448 1166
Size: 2282 4946
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7228
AlwNI CAG_nnn'CTG
Cuts at: 2250 2528 2615 4971 6543 2250
Size: 278 87 2356 1572 2935
Fragments arranged by size:
2935 2356 1572 278 87
ApaLI G'TGCA_C
Cuts at: 4037 5285 6441 4037
Size: 1248 1156 4824
Fragments arranged by size:
4824 1248 1156
ApoI r'AATT_y
Cuts at: 1493 2547 2724 2807 3439 4537 4763 4915 7024
Size: 1054 177 83 632 1098 226 152 2109
Cuts at: 7024 1493
Size: 1697
Fragments arranged by size:
2109 1697 1098 1054 632 226 177 152
83
AseI AT'TA_AT
Cuts at: 160 1032 5782 160
Size: 872 4750 1606
Fragments arranged by size:
4750 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7228
AvaI C'yCGr_G
Cuts at: 1870 1943 2843 2967 3586 3658 4285 4553 1870
Size: 73 900 124 619 72 627 268 4545
Fragments arranged by size:
4545 900 627 619 268 124 73 72
BamHI G'GATC_C
Cuts at: 2839 4558 4925 2839
Size: 1719 367 5142
Fragments arranged by size:
5142 1719 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2573 2762 4375 4549 6004
Size: 325 217 627 786 189 1613 174 1455
Cuts at: 6004 618
Size: 1842
Fragments arranged by size:
1842 1613 1455 786 627 325 217 189
174
BanII G_rGCy'C
Cuts at: 1391 3077 3207 4064 4086 4547 1391
Size: 1686 130 857 22 461 4072
Fragments arranged by size:
4072 1686 857 461 130 22
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2305 3057 3876 4227 928
Size: 971 406 752 819 351 3929
Fragments arranged by size:
3929 971 819 752 406 351
BbvCI CC'TCA_GC
Cuts at: 3710 3710
Size: 7228
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5419 5453 1903
Size: 34 3482 34 3678
Fragments arranged by size:
3678 3482 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5120 6330 5120
Size: 1210 6018
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7228
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5839 136
Size: 107 122 71 631 605 4167 1525
Fragments arranged by size:
4167 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2706 7223 2706
Size: 4517 2711
BlpI GC'TnA_GC
Cuts at: 2894 4645 2894
Size: 1751 5477
Bme1580I G_kGCm'C
Cuts at: 1790 2049 4041 4182 4380 5289 6445 1790
Size: 259 1992 141 198 909 1156 2573
Fragments arranged by size:
2573 1992 1156 909 259 198 141
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7228
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2043 3546 4959
Size: 416 53 83 399 24 626 1503 1413
Cuts at: 4959 5917 442
Size: 958 1753
Fragments arranged by size:
1753 1503 1413 958 626 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7228
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3248 3248
Size: 7228
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2316 2325 2446 3183 4100 5888 1239
Size: 1077 9 121 737 917 1788 2579
Fragments arranged by size:
2579 1788 1077 917 737 121 9
Bpu10I CC'TnA_GC
Cuts at: 3710 3710
Size: 7228
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5353 6218 6516 5353
Size: 865 298 6065
Fragments arranged by size:
6065 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5891 882
Size: 617 4392 2219
Fragments arranged by size:
4392 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4525 5019 493
Size: 711 3321 494 2702
Fragments arranged by size:
3321 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 7222 7222
Size: 7228
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2094 5035 5417 6052
Size: 53 83 186 936 561 2941 382 635
Cuts at: 6052 275
Size: 1451
Fragments arranged by size:
2941 1451 936 635 561 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2053 3217 4372 5661 6059 6333 6480 6891 2053
Size: 1164 1155 1289 398 274 147 411 2390
Fragments arranged by size:
2390 1289 1164 1155 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4382 4412 1178
Size: 30 165 30 2979 30 3994
Fragments arranged by size:
3994 2979 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2611 2930 3886 3889 3892 3895 3898 3901
Size: 1425 319 956 3 3 3 3 3
Cuts at: 3901 3904 4390 1186
Size: 3 486 4024
Fragments arranged by size:
4024 1425 956 486 319 3 3 3
3 3 3
BseYI C'CCAG_C
Cuts at: 2212 4358 6431 6927 2212
Size: 2146 2073 496 2513
Fragments arranged by size:
2513 2146 2073 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2263 1163
Size: 573 527 6128
Fragments arranged by size:
6128 573 527
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4678 5439 6467 1540
Size: 275 186 2677 761 1028 2301
Fragments arranged by size:
2677 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3077 4041 5289 5374 6445 3077
Size: 964 1248 85 1071 3860
Fragments arranged by size:
3860 1248 1071 964 85
BsmI GAATG_Cn'
Cuts at: 3985 4739 4832 3985
Size: 754 93 6381
Fragments arranged by size:
6381 754 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2647 3945 2647
Size: 1298 5930
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2049 3077 3207 4041 4064 4086 4182
Size: 399 259 1028 130 834 23 22 96
Cuts at: 4182 4380 4547 5289 5374 6445 1391
Size: 198 167 742 85 1071 2174
Fragments arranged by size:
2174 1071 1028 834 742 399 259 198
167 130 96 85 23 22
BspEI T'CCGG_A
Cuts at: 2053 6891 2053
Size: 4838 2390
BspHI T'CATG_A
Cuts at: 5117 6847 5117
Size: 1730 5498
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2885 4399 4583 844
Size: 2041 1514 184 3489
Fragments arranged by size:
3489 2041 1514 184
BsrBI CCG'CTC
Cuts at: 1394 1823 5115 1394
Size: 429 3292 3507
Fragments arranged by size:
3507 3292 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5723 5897 65
Size: 1183 4475 174 1396
Fragments arranged by size:
4475 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3040 3217 4372 5872 6059 1700
Size: 110 1230 177 1155 1500 187 2869
Fragments arranged by size:
2869 1500 1230 1155 187 177 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7228
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7228
BssSI C'ACGA_G
Cuts at: 3010 5288 6300 3010
Size: 2278 1012 3938
Fragments arranged by size:
3938 2278 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2573 2615 3104 794
Size: 1779 42 489 4918
Fragments arranged by size:
4918 1779 489 42
BstBI TT'CG_AA
Cuts at: 2007 6032 2007
Size: 4025 3203
BstEII G'GTnAC_C
Cuts at: 1184 3227 1184
Size: 2043 5185
BstXI CCAn_nnnn'nTGG
Cuts at: 3328 3328
Size: 7228
BstYI r'GATC_y
Cuts at: 1972 2706 2839 2867 3459 4558 4925 5310 5327
Size: 734 133 28 592 1099 367 385 17
Cuts at: 5327 6779 6865 6877 7223 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1099 734 592 385 367 346
133 86 28 17 12
Bsu36I CC'TnA_GG
Cuts at: 4087 4087
Size: 7228
BtgI C'CryG_G
Cuts at: 513 1065 2022 3420 4116 513
Size: 552 957 1398 696 3625
Fragments arranged by size:
3625 1398 957 696 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3633 487
Size: 683 56 2407 4082
Fragments arranged by size:
4082 2407 683 56
BtsI GCAGTG_nn'
Cuts at: 2107 2251 2287 4162 4744 5538 5558 2107
Size: 144 36 1875 582 794 20 3777
Fragments arranged by size:
3777 1875 794 582 144 36 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7228
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7193
DraI TTT'AAA
Cuts at: 4532 4532
Size: 7228
DraIII CAC_nnn'GTG
Cuts at: 4070 4070
Size: 7228
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6235 809
Size: 507 4919 1802
Fragments arranged by size:
4919 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2203 2398 3190
Size: 54 1425 50 275 18 373 195 792
Cuts at: 3190 3699 4675 5564 7193 8
Size: 509 976 889 1629 43
Fragments arranged by size:
1629 1425 976 889 792 509 373 275
195 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4675 1537
Size: 275 2863 4090
Fragments arranged by size:
4090 2863 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2127 3604 3922 4521 5158 1353
Size: 774 1477 318 599 637 3423
Fragments arranged by size:
3423 1477 774 637 599 318
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3733 5815 6189 6335 387
Size: 1064 138 2144 2082 374 146 1280
Fragments arranged by size:
2144 2082 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3075 3075
Size: 7228
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2316 2325 2446 3051 3183 3555 4100
Size: 393 684 9 121 605 132 372 545
Cuts at: 4100 5291 5888 6675 6943 1239
Size: 1191 597 787 268 1524
Fragments arranged by size:
1524 1191 787 684 605 597 545 393
372 268 132 121 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2493 2561 2493
Size: 68 7160
EcoO109I rG'GnC_Cy
Cuts at: 3736 3953 4204 3736
Size: 217 251 6760
Fragments arranged by size:
6760 251 217
EcoRI G'AATT_C
Cuts at: 2724 3439 4537 4915 2724
Size: 715 1098 378 5037
Fragments arranged by size:
5037 1098 715 378
FspI TGC'GCA
Cuts at: 5734 5734
Size: 7228
HaeII r_GCGC'y
Cuts at: 1678 3666 6375 1678
Size: 1988 2709 2531
Fragments arranged by size:
2709 2531 1988
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2020 2052 4498 4530 5869
Size: 32 135 32 448 32 2446 32 1339
Cuts at: 5869 5901 5943 5975 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2446 1339 448 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4390 4572 4818 669
Size: 988 281 2452 182 246 3079
Fragments arranged by size:
3079 2452 988 281 246 182
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7228
HpaI GTT'AAC
Cuts at: 4390 4818 4390
Size: 428 6800
KpnI G_GTAC'C
Cuts at: 4553 4553
Size: 7228
MfeI C'AATT_G
Cuts at: 4827 4827
Size: 7228
MluI A'CGCG_T
Cuts at: 7104 7104
Size: 7228
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2139 3202 6342 6526 1305
Size: 834 1063 3140 184 2007
Fragments arranged by size:
3140 2007 1063 834 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2205 10
Size: 54 1425 343 373 5033
Fragments arranged by size:
5033 1425 373 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2757 3448 4319 5186 5545 5704 518
Size: 648 1591 691 871 867 359 159 2042
Fragments arranged by size:
2042 1591 871 867 691 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2397 4118 4644 5321 6469 6714 1606
Size: 791 1721 526 677 1148 245 2120
Fragments arranged by size:
2120 1721 1148 791 677 526 245
NaeI GCC'GGC
Cuts at: 3042 3042
Size: 7228
NcoI C'CATG_G
Cuts at: 513 1065 2022 3420 513
Size: 552 957 1398 4321
Fragments arranged by size:
4321 1398 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7228
NgoMIV G'CCGG_C
Cuts at: 3040 3040
Size: 7228
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7228
NotI GC'GGCC_GC
Cuts at: 4675 4675
Size: 7228
NspI r_CATG'y
Cuts at: 1132 1229 4586 6131 1132
Size: 97 3357 1545 2229
Fragments arranged by size:
3357 2229 1545 97
PacI TTA_AT'TAA
Cuts at: 2661 2661
Size: 7228
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7228
PciI A'CATG_T
Cuts at: 6127 6127
Size: 7228
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3221 1108
Size: 2113 5115
PfoI T'CCnGG_A
Cuts at: 2084 2084
Size: 7228
PmeI GTTT'AAAC
Cuts at: 4532 4532
Size: 7228
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5297 5329 6810 6842 5297
Size: 32 1481 32 5683
Fragments arranged by size:
5683 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4204 4204
Size: 7228
PshAI GACnn'nnGTC
Cuts at: 1613 4164 1613
Size: 2551 4677
PsiI TTA'TAA
Cuts at: 4798 4798
Size: 7228
PspXI vC'TCGA_Gb
Cuts at: 3586 4285 3586
Size: 699 6529
PstI C_TGCA'G
Cuts at: 830 2611 4580 830
Size: 1781 1969 3478
Fragments arranged by size:
3478 1969 1781
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7228
SacI G_AGCT'C
Cuts at: 3077 3077
Size: 7228
SacII CC_GC'GG
Cuts at: 4119 4119
Size: 7228
SalI G'TCGA_C
Cuts at: 1936 4570 1936
Size: 2634 4594
SbfI CC_TGCA'GG
Cuts at: 4580 4580
Size: 7228
ScaI AGT'ACT
Cuts at: 5476 7188 5476
Size: 1712 5516
SexAI A'CCwGG_T
Cuts at: 2256 2256
Size: 7228
SfcI C'TryA_G
Cuts at: 826 1045 2529 2607 4576 5711 6392 6583 826
Size: 219 1484 78 1969 1135 681 191 1471
Fragments arranged by size:
1969 1484 1471 1135 681 219 191 78
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7228
SgrAI Cr'CCGG_yG
Cuts at: 1700 4372 1700
Size: 2672 4556
SmaI CCC'GGG
Cuts at: 1872 2969 4555 1872
Size: 1097 1586 4545
Fragments arranged by size:
4545 1586 1097
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3586 4285 5332 6233 6495 820
Size: 197 926 1643 699 1047 901 262 1553
Fragments arranged by size:
1643 1553 1047 926 901 699 262 197
SnaBI TAC'GTA
Cuts at: 493 4525 5019 493
Size: 4032 494 2702
Fragments arranged by size:
4032 2702 494
SpeI A'CTAG_T
Cuts at: 152 2365 152
Size: 2213 5015
SphI G_CATG'C
Cuts at: 1132 4586 1132
Size: 3454 3774
SspI AAT'ATT
Cuts at: 5 52 5152 5
Size: 47 5100 2081
Fragments arranged by size:
5100 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7228
StyI C'CwwG_G
Cuts at: 513 1065 2022 3420 4667 513
Size: 552 957 1398 1247 3074
Fragments arranged by size:
3074 1398 1247 957 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3136 5255 5272 5425 5610 6055 325
Size: 1255 1556 2119 17 153 185 445 1498
Fragments arranged by size:
2119 1556 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2368 3130 5474
Size: 274 80 33 51 1443 391 762 2344
Cuts at: 5474 7186 96
Size: 1712 138
Fragments arranged by size:
2344 1712 1443 762 391 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2262 2294 2470 2502 3502 3534 3776 3808 7188
Size: 32 176 32 1000 32 242 32 3380
Cuts at: 7188 7220 2262
Size: 32 2270
Fragments arranged by size:
3380 2270 1000 242 176 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7228
XbaI T'CTAG_A
Cuts at: 4564 4564
Size: 7228
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2029 2029
Size: 7228
XhoI C'TCGA_G
Cuts at: 1943 3586 4285 1943
Size: 1643 699 4886
Fragments arranged by size:
4886 1643 699
XmaI C'CCGG_G
Cuts at: 1870 2967 4553 1870
Size: 1097 1586 4545
Fragments arranged by size:
4545 1586 1097
XmnI GAAnn'nnTTC
Cuts at: 3021 5357 3021
Size: 2336 4892
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5036 276
Size: 53 83 186 936 3502 2468
Fragments arranged by size:
3502 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PacI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspXI PstI PvuI SacI SacII SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BstZ17I EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PmlI PspOMI PsrI PvuII RsrII SanDI
SapI SfiI SfoI SrfI SwaI