Restriction map for
pFN21ASDA1215
(Circular) (Six-base) MAPSORT of: pFN21ASDA1215.seq Check: 6205 from: 1 to: 6292
pFN21ASDA1215 6292 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4102 278
Size: 53 83 186 936 2566 2468
Fragments arranged by size:
2566 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2877 3635 1937
Size: 940 758 4594
Fragments arranged by size:
4594 940 758
Acc65I G'GTAC_C
Cuts at: 3613 3613
Size: 6292
AclI AA'CG_TT
Cuts at: 1563 2808 4419 4792 1563
Size: 1245 1611 373 3063
Fragments arranged by size:
3063 1611 1245 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3460 4355 5739 6007 1632
Size: 1828 895 1384 268 1917
Fragments arranged by size:
1917 1828 1384 895 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6095
AflIII A'CryG_T
Cuts at: 1203 5191 6168 1203
Size: 3988 977 1327
Fragments arranged by size:
3988 1327 977
AgeI A'CCGG_T
Cuts at: 5123 5123
Size: 6292
AhdI GACnn_n'nnGTC
Cuts at: 2032 2191 5021 2032
Size: 159 2830 3303
Fragments arranged by size:
3303 2830 159
AleI CACnn'nnGTG
Cuts at: 1166 2343 2419 1166
Size: 1177 76 5039
Fragments arranged by size:
5039 1177 76
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6292
AlwNI CAG_nnn'CTG
Cuts at: 4035 5607 4035
Size: 1572 4720
ApaLI G'TGCA_C
Cuts at: 2708 4349 5505 2708
Size: 1641 1156 3495
Fragments arranged by size:
3495 1641 1156
ApoI r'AATT_y
Cuts at: 1493 3296 3527 3601 3827 3979 6088 1493
Size: 1803 231 74 226 152 2109 1697
Fragments arranged by size:
2109 1803 1697 231 226 152 74
AseI AT'TA_AT
Cuts at: 160 1032 4846 160
Size: 872 3814 1606
Fragments arranged by size:
3814 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6292
AvaI C'yCGr_G
Cuts at: 1870 1943 2044 2168 2643 2991 3183 3617 1870
Size: 73 101 124 475 348 192 434 4545
Fragments arranged by size:
4545 475 434 348 192 124 101 73
BamHI G'GATC_C
Cuts at: 2844 3535 3622 3989 2844
Size: 691 87 367 5147
Fragments arranged by size:
5147 691 367 87
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2252 2789 3613 5068 618
Size: 325 217 627 465 537 824 1455 1842
Fragments arranged by size:
1842 1455 824 627 537 465 325 217
BanII G_rGCy'C
Cuts at: 1391 2178 2518 3611 1391
Size: 787 340 1093 4072
Fragments arranged by size:
4072 1093 787 340
BbeI G_GCGC'C
Cuts at: 2793 2793
Size: 6292
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5321
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4483 4517 1903
Size: 34 2546 34 3678
Fragments arranged by size:
3678 2546 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4184 5394 4184
Size: 1210 5082
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6292
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2366 4903 136
Size: 107 122 71 631 605 694 2537 1525
Fragments arranged by size:
2537 1525 694 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6287 6287
Size: 6292
BlpI GC'TnA_GC
Cuts at: 3060 3709 3060
Size: 649 5643
Bme1580I G_kGCm'C
Cuts at: 1790 2255 2572 2712 4353 5509 1790
Size: 465 317 140 1641 1156 2573
Fragments arranged by size:
2573 1641 1156 465 317 140
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6292
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2032 2233 2513
Size: 416 53 83 399 24 615 201 280
Cuts at: 2513 4023 4981 442
Size: 1510 958 1753
Fragments arranged by size:
1753 1510 958 615 416 399 280 201
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6292
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2456 2732 2753 2870 4952 1239
Size: 1217 276 21 117 2082 2579
Fragments arranged by size:
2579 2082 1217 276 117 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2750 3422 4417 5282 5580 2750
Size: 672 995 865 298 3462
Fragments arranged by size:
3462 995 865 672 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2608 3569 4955 882
Size: 617 1109 961 1386 2219
Fragments arranged by size:
2219 1386 1109 961 617
BsaAI yAC'GTr
Cuts at: 493 1204 3589 4083 493
Size: 711 2385 494 2702
Fragments arranged by size:
2702 2385 711 494
BsaBI GATnn'nnATC
Cuts at: 6286 6286
Size: 6292
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2694 2790 3320 4099
Size: 53 83 186 936 1161 96 530 779
Cuts at: 4099 4481 5116 275
Size: 382 635 1451
Fragments arranged by size:
1451 1161 936 779 635 530 382 186
96 83 53
BsaWI w'CCGG_w
Cuts at: 4725 5123 5397 5544 5955 4725
Size: 398 274 147 411 5062
Fragments arranged by size:
5062 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2255 2285 1178
Size: 30 165 30 852 30 5185
Fragments arranged by size:
5185 852 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2444 2882 3068 3100 3128 3164 3431 1186
Size: 1258 438 186 32 28 36 267 4047
Fragments arranged by size:
4047 1258 438 267 186 36 32 28
BseYI C'CCAG_C
Cuts at: 2511 5495 5991 2511
Size: 2984 496 2812
Fragments arranged by size:
2984 2812 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2690 1163
Size: 573 954 4765
Fragments arranged by size:
4765 954 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3742 4503 5531 1540
Size: 275 186 1741 761 1028 2301
Fragments arranged by size:
2301 1741 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2265 2712 4353 4438 5509 2265
Size: 447 1641 85 1071 3048
Fragments arranged by size:
3048 1641 1071 447 85
BsmI GAATG_Cn'
Cuts at: 3803 3896 3803
Size: 93 6199
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2178 2255 2265 2518 2572 2712 3611
Size: 399 388 77 10 253 54 140 899
Cuts at: 3611 4353 4438 5509 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 899 742 399 388 253 140
85 77 54 10
BspEI T'CCGG_A
Cuts at: 5955 5955
Size: 6292
BspHI T'CATG_A
Cuts at: 3558 4181 5911 3558
Size: 623 1730 3939
Fragments arranged by size:
3939 1730 623
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2907 3647 844
Size: 2063 740 3489
Fragments arranged by size:
3489 2063 740
BsrBI CCG'CTC
Cuts at: 1394 1823 2115 2212 4179 1394
Size: 429 292 97 1967 3507
Fragments arranged by size:
3507 1967 429 292 97
BsrDI GCAATG_nn'
Cuts at: 65 1248 4787 4961 65
Size: 1183 3539 174 1396
Fragments arranged by size:
3539 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2529 3421 4936 5123 1700
Size: 110 719 892 1515 187 2869
Fragments arranged by size:
2869 1515 892 719 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6292
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6292
BssSI C'ACGA_G
Cuts at: 2199 2597 4352 5364 2199
Size: 398 1755 1012 3127
Fragments arranged by size:
3127 1755 1012 398
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6292
BstBI TT'CG_AA
Cuts at: 2007 5096 2007
Size: 3089 3203
BstEII G'GTnAC_C
Cuts at: 1184 3424 1184
Size: 2240 4052
BstXI CCAn_nnnn'nTGG
Cuts at: 2305 2421 2305
Size: 116 6176
BstYI r'GATC_y
Cuts at: 1972 2844 3535 3622 3989 4374 4391 5843 5929
Size: 872 691 87 367 385 17 1452 86
Cuts at: 5929 5941 6287 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 872 691 385 367 346 87
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2721 3213 3270 513
Size: 552 957 699 492 57 3535
Fragments arranged by size:
3535 957 699 552 492 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2278 487
Size: 683 56 1052 4501
Fragments arranged by size:
4501 1052 683 56
BtsI GCAGTG_nn'
Cuts at: 2369 2414 2844 3808 4602 4622 2369
Size: 45 430 964 794 20 4039
Fragments arranged by size:
4039 964 794 430 45 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6292
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6257
DraI TTT'AAA
Cuts at: 3596 3596
Size: 6292
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2873 5299 809
Size: 507 1557 2426 1802
Fragments arranged by size:
2426 1802 1557 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2718 2812 2916
Size: 54 1425 50 275 18 888 94 104
Cuts at: 2916 2983 3390 3739 4628 6257 8
Size: 67 407 349 889 1629 43
Fragments arranged by size:
1629 1425 889 888 407 349 275 104
94 67 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3739 1537
Size: 275 1927 4090
Fragments arranged by size:
4090 1927 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2471 2621 3141 4222 1353
Size: 1118 150 520 1081 3423
Fragments arranged by size:
3423 1118 1081 520 150
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2516 4879 5253 5399 387
Size: 1064 138 927 2363 374 146 1280
Fragments arranged by size:
2363 1280 1064 927 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2456 2732 2753 2870 3460 4355 4952
Size: 393 824 276 21 117 590 895 597
Cuts at: 4952 5739 6007 1239
Size: 787 268 1524
Fragments arranged by size:
1524 895 824 787 597 590 393 276
268 117 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2954 2954
Size: 6292
EcoO109I rG'GnC_Cy
Cuts at: 2461 3502 2461
Size: 1041 5251
EcoRI G'AATT_C
Cuts at: 3296 3601 3979 3296
Size: 305 378 5609
Fragments arranged by size:
5609 378 305
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3349 3349
Size: 6292
FspI TGC'GCA
Cuts at: 2630 3080 4798 2630
Size: 450 1718 4124
Fragments arranged by size:
4124 1718 450
FspAI rTGC'GCAy
Cuts at: 2630 2630
Size: 6292
HaeII r_GCGC'y
Cuts at: 1678 2793 5439 1678
Size: 1115 2646 2531
Fragments arranged by size:
2646 2531 1115
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2018 2050 2177 2209 3348
Size: 32 135 32 446 32 127 32 1139
Cuts at: 3348 3380 3527 3559 4933 4965 5007 5039 1373
Size: 32 147 32 1374 32 42 32 2626
Fragments arranged by size:
2626 1374 1139 446 147 135 127 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3636 3882 669
Size: 988 281 1698 246 3079
Fragments arranged by size:
3079 1698 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6292
HpaI GTT'AAC
Cuts at: 3882 3882
Size: 6292
KasI G'GCGC_C
Cuts at: 2789 2789
Size: 6292
KpnI G_GTAC'C
Cuts at: 3617 3617
Size: 6292
MfeI C'AATT_G
Cuts at: 3891 3891
Size: 6292
MluI A'CGCG_T
Cuts at: 6168 6168
Size: 6292
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5406 5590 1305
Size: 4101 184 2007
Fragments arranged by size:
4101 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2814 2918 2985 3392 10
Size: 54 1425 343 982 104 67 407 2910
Fragments arranged by size:
2910 1425 982 407 343 104 67 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2343 2419 4250 4609 4768 518
Size: 648 1177 76 1831 359 159 2042
Fragments arranged by size:
2042 1831 1177 648 359 159 76
MspA1I CmG'CkG
Cuts at: 1606 2354 2511 3008 3708 4385 5533 5778 1606
Size: 748 157 497 700 677 1148 245 2120
Fragments arranged by size:
2120 1148 748 700 677 497 245 157
NaeI GCC'GGC
Cuts at: 2531 2531
Size: 6292
NarI GG'CG_CC
Cuts at: 2790 2790
Size: 6292
NcoI C'CATG_G
Cuts at: 513 1065 2022 3213 3270 513
Size: 552 957 1191 57 3535
Fragments arranged by size:
3535 1191 957 552 57
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6292
NgoMIV G'CCGG_C
Cuts at: 2529 2529
Size: 6292
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6292
NotI GC'GGCC_GC
Cuts at: 3739 3739
Size: 6292
NspI r_CATG'y
Cuts at: 1132 1229 3650 5195 1132
Size: 97 2421 1545 2229
Fragments arranged by size:
2421 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3165 1348
Size: 1817 4475
PciI A'CATG_T
Cuts at: 5191 5191
Size: 6292
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2665 2833 3271 3399 1108
Size: 1557 168 438 128 4001
Fragments arranged by size:
4001 1557 438 168 128
PfoI T'CCnGG_A
Cuts at: 2634 2847 2634
Size: 213 6079
PmeI GTTT'AAAC
Cuts at: 3596 3596
Size: 6292
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4361 4393 5874 5906 4361
Size: 32 1481 32 4747
Fragments arranged by size:
4747 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3502 3502
Size: 6292
PshAI GACnn'nnGTC
Cuts at: 1613 3030 1613
Size: 1417 4875
PsiI TTA'TAA
Cuts at: 3862 3862
Size: 6292
PstI C_TGCA'G
Cuts at: 830 3144 3288 3570 3644 830
Size: 2314 144 282 74 3478
Fragments arranged by size:
3478 2314 282 144 74
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6292
PvuII CAG'CTG
Cuts at: 2511 2511
Size: 6292
SalI G'TCGA_C
Cuts at: 1936 3634 1936
Size: 1698 4594
SapI GCTCTTCn'nnn_
Cuts at: 2621 2621
Size: 6292
SbfI CC_TGCA'GG
Cuts at: 3644 3644
Size: 6292
ScaI AGT'ACT
Cuts at: 2042 3044 4540 6252 2042
Size: 1002 1496 1712 2082
Fragments arranged by size:
2082 1712 1496 1002
SfcI C'TryA_G
Cuts at: 826 1045 3140 3284 3566 3640 4775 5456 5647
Size: 219 2095 144 282 74 1135 681 191
Cuts at: 5647 826
Size: 1471
Fragments arranged by size:
2095 1471 1135 681 282 219 191 144
74
SfoI GGC'GCC
Cuts at: 2791 2791
Size: 6292
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6292
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6292
SmaI CCC'GGG
Cuts at: 1872 2170 3185 3619 1872
Size: 298 1015 434 4545
Fragments arranged by size:
4545 1015 434 298
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2765 3437 4396 5297 5559 820
Size: 197 926 822 672 959 901 262 1553
Fragments arranged by size:
1553 959 926 901 822 672 262 197
SnaBI TAC'GTA
Cuts at: 493 3589 4083 493
Size: 3096 494 2702
Fragments arranged by size:
3096 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6292
SphI G_CATG'C
Cuts at: 1132 3650 1132
Size: 2518 3774
SspI AAT'ATT
Cuts at: 5 52 4216 5
Size: 47 4164 2081
Fragments arranged by size:
4164 2081 47
StuI AGG'CCT
Cuts at: 1517 2540 3119 3329 1517
Size: 1023 579 210 4480
Fragments arranged by size:
4480 1023 579 210
StyI C'CwwG_G
Cuts at: 513 1065 2022 2298 2535 3213 3270 3324 3731
Size: 552 957 276 237 678 57 54 407
Cuts at: 3731 513
Size: 3074
Fragments arranged by size:
3074 957 678 552 407 276 237 57
54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2121 2333 4319 4336 4489 4674 5119
Size: 1255 541 212 1986 17 153 185 445
Cuts at: 5119 325
Size: 1498
Fragments arranged by size:
1986 1498 1255 541 445 212 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2040 3042 4538
Size: 274 80 33 51 1443 63 1002 1496
Cuts at: 4538 6250 96
Size: 1712 138
Fragments arranged by size:
1712 1496 1443 1002 274 138 80 63
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2255 2287 6252 6284 2255
Size: 32 3965 32 2263
Fragments arranged by size:
3965 2263 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2315 1316
Size: 999 5293
XbaI T'CTAG_A
Cuts at: 3628 3628
Size: 6292
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2911 3100 3172 3331 2911
Size: 189 72 159 5872
Fragments arranged by size:
5872 189 159 72
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6292
XmaI C'CCGG_G
Cuts at: 1870 2168 3183 3617 1870
Size: 298 1015 434 4545
Fragments arranged by size:
4545 1015 434 298
XmnI GAAnn'nnTTC
Cuts at: 4421 4421
Size: 6292
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4100 276
Size: 53 83 186 936 2566 2468
Fragments arranged by size:
2566 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoRV FseI NruI NsiI PacI PmlI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI
SrfI SwaI