Restriction map for
pFN21ASDA1205
(Circular) (Six-base) MAPSORT of: pFN21ASDA1205.seq Check: 3992 from: 1 to: 8374
pFN21ASDA1205 8374 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2805 2805
Size: 8374
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6184 278
Size: 53 83 186 936 4648 2468
Fragments arranged by size:
4648 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5717 1937
Size: 3780 4594
Acc65I G'GTAC_C
Cuts at: 4482 5695 4482
Size: 1213 7161
AclI AA'CG_TT
Cuts at: 1563 6501 6874 1563
Size: 4938 373 3063
Fragments arranged by size:
4938 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3245 3689 4985 5270 5608 6437 7821 8089
Size: 1613 444 1296 285 338 829 1384 268
Cuts at: 8089 1632
Size: 1917
Fragments arranged by size:
1917 1613 1384 1296 829 444 338 285
268
AflII C'TTAA_G
Cuts at: 820 1017 3233 820
Size: 197 2216 5961
Fragments arranged by size:
5961 2216 197
AflIII A'CryG_T
Cuts at: 1203 4802 5292 7273 8250 1203
Size: 3599 490 1981 977 1327
Fragments arranged by size:
3599 1981 1327 977 490
AgeI A'CCGG_T
Cuts at: 7205 7205
Size: 8374
AhdI GACnn_n'nnGTC
Cuts at: 5026 7103 5026
Size: 2077 6297
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8374
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4452 1184
Size: 3268 5106
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2402 2434 2843 2875 2402
Size: 32 409 32 7901
Fragments arranged by size:
7901 409 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3305 4118 5069 5167 5527 5661 6117 7689 3305
Size: 813 951 98 360 134 456 1572 3990
Fragments arranged by size:
3990 1572 951 813 456 360 134 98
ApaI G_GGCC'C
Cuts at: 2535 2568 3120 5003 2535
Size: 33 552 1883 5906
Fragments arranged by size:
5906 1883 552 33
ApaLI G'TGCA_C
Cuts at: 6431 7587 6431
Size: 1156 7218
ApoI r'AATT_y
Cuts at: 1493 4397 5683 5909 6061 8170 1493
Size: 2904 1286 226 152 2109 1697
Fragments arranged by size:
2904 2109 1697 1286 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6928 160
Size: 872 5896 1606
Fragments arranged by size:
5896 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8374
AvaI C'yCGr_G
Cuts at: 1870 1943 2108 2168 2210 2567 3159 3325 3336
Size: 73 165 60 42 357 592 166 11
Cuts at: 3336 3516 3680 3902 4172 4745 5699 1870
Size: 180 164 222 270 573 954 4545
Fragments arranged by size:
4545 954 592 573 357 270 222 180
166 165 164 73 60 42 11
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2238 2271 2238
Size: 33 8341
BamHI G'GATC_C
Cuts at: 2182 2281 3084 5704 6071 2182
Size: 99 803 2620 367 4485
Fragments arranged by size:
4485 2620 803 367 99
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2041 2192 2236 2291 2318
Size: 325 217 627 254 151 44 55 27
Cuts at: 2318 2326 2671 2805 3018 3146 3206 3593 4482
Size: 8 345 134 213 128 60 387 889
Cuts at: 4482 5543 5695 7150 618
Size: 1061 152 1455 1842
Fragments arranged by size:
1842 1455 1061 889 627 387 345 325
254 217 213 152 151 134 128 60
55 44 27 8
BanII G_rGCy'C
Cuts at: 1391 2142 2218 2535 2568 3120 3222 3876 3909
Size: 751 76 317 33 552 102 654 33
Cuts at: 3909 4149 4737 5003 5044 5333 5425 5693 1391
Size: 240 588 266 41 289 92 268 4072
Fragments arranged by size:
4072 751 654 588 552 317 289 268
266 240 102 92 76 41 33 33
BbeI G_GCGC'C
Cuts at: 2196 2240 2295 2330 2675 2809 3022 3150 3597
Size: 44 55 35 345 134 213 128 447
Cuts at: 3597 5547 2196
Size: 1950 5023
Fragments arranged by size:
5023 1950 447 345 213 134 128 55
44 35
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3720 4173 928
Size: 971 1821 453 5129
Fragments arranged by size:
5129 1821 971 453
BbvCI CC'TCA_GC
Cuts at: 3192 3960 3966 4311 3192
Size: 768 6 345 7255
Fragments arranged by size:
7255 768 345 6
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6565 6599 1903
Size: 34 4628 34 3678
Fragments arranged by size:
4628 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2239 5450 6266 7476 2239
Size: 3211 816 1210 3137
Fragments arranged by size:
3211 3137 1210 816
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8374
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2316 3064 3602
Size: 107 122 71 631 605 644 748 538
Cuts at: 3602 3839 4127 5007 6985 136
Size: 237 288 880 1978 1525
Fragments arranged by size:
1978 1525 880 748 644 631 605 538
288 237 122 107 71
BglII A'GATC_T
Cuts at: 8369 8369
Size: 8374
BlpI GC'TnA_GC
Cuts at: 3479 5264 5393 5791 3479
Size: 1785 129 398 6062
Fragments arranged by size:
6062 1785 398 129
Bme1580I G_kGCm'C
Cuts at: 1790 2535 2568 3120 3209 3255 5003 6435 7591
Size: 745 33 552 89 46 1748 1432 1156
Cuts at: 7591 1790
Size: 2573
Fragments arranged by size:
2573 1748 1432 1156 745 552 89 46
33
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8374
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2978 3873 4565
Size: 416 53 83 399 24 1561 895 692
Cuts at: 4565 6105 7063 442
Size: 1540 958 1753
Fragments arranged by size:
1753 1561 1540 958 895 692 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8374
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3351 3733 3769 4771 3351
Size: 382 36 1002 6954
Fragments arranged by size:
6954 1002 382 36
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2156 2240 2920 3116 3620 3938 5058 5189
Size: 917 84 680 196 504 318 1120 131
Cuts at: 5189 7034 1239
Size: 1845 2579
Fragments arranged by size:
2579 1845 1120 917 680 504 318 196
131 84
Bpu10I CC'TnA_GC
Cuts at: 3192 3960 3966 4311 4731 3192
Size: 768 6 345 420 6835
Fragments arranged by size:
6835 768 420 345 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2762 3410 4313 5387 6499 7364 7662 2762
Size: 648 903 1074 1112 865 298 3474
Fragments arranged by size:
3474 1112 1074 903 865 648 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2938 3729 4664 7037 882
Size: 617 1439 791 935 2373 2219
Fragments arranged by size:
2373 2219 1439 935 791 617
BsaAI yAC'GTr
Cuts at: 493 1204 4534 4805 5119 5671 6165 493
Size: 711 3330 271 314 552 494 2702
Fragments arranged by size:
3330 2702 711 552 494 314 271
BsaBI GATnn'nnATC
Cuts at: 8368 8368
Size: 8374
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2193 2237 2292 2327
Size: 53 83 186 936 660 44 55 35
Cuts at: 2327 2552 2672 2806 3019 3147 3470 3594 5074
Size: 225 120 134 213 128 323 124 1480
Cuts at: 5074 5544 6181 6563 7198 275
Size: 470 637 382 635 1451
Fragments arranged by size:
1480 1451 936 660 637 635 470 382
323 225 213 186 134 128 124 120
83 55 53 44 35
BsaWI w'CCGG_w
Cuts at: 3572 4405 5109 5335 6807 7205 7479 7626 8037
Size: 833 704 226 1472 398 274 147 411
Cuts at: 8037 3572
Size: 3909
Fragments arranged by size:
3909 1472 833 704 411 398 274 226
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2402 2432 2843 2873 2918
Size: 30 165 30 999 30 411 30 45
Cuts at: 2918 2948 3377 3407 3928 3958 1178
Size: 30 429 30 521 30 5594
Fragments arranged by size:
5594 999 521 429 411 165 45 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2186 2399 2426 3209 3386 3677 3691 3745
Size: 1000 213 27 783 177 291 14 54
Cuts at: 3745 3935 3948 4125 4157 4262 5005 5422 5579
Size: 190 13 177 32 105 743 417 157
Cuts at: 5579 1186
Size: 3981
Fragments arranged by size:
3981 1000 783 743 417 291 213 190
177 177 157 105 54 32 27 14
13
BseYI C'CCAG_C
Cuts at: 2150 2306 2492 2638 3531 3791 4037 4616 4648
Size: 156 186 146 893 260 246 579 32
Cuts at: 4648 5045 5269 5366 7577 8073 2150
Size: 397 224 97 2211 496 2451
Fragments arranged by size:
2451 2211 893 579 496 397 260 246
224 186 156 146 97 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4614 5273 1163
Size: 573 2878 659 4264
Fragments arranged by size:
4264 2878 659 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2072 5824 6585 7613 1540
Size: 275 186 71 3752 761 1028 2301
Fragments arranged by size:
3752 2301 1028 761 275 186 71
BsiHKAI G_wGCw'C
Cuts at: 4549 5138 5235 6435 6520 7591 4549
Size: 589 97 1200 85 1071 5332
Fragments arranged by size:
5332 1200 1071 589 97 85
BsmI GAATG_Cn'
Cuts at: 5885 5978 5885
Size: 93 8281
BsmBI CGTCTCn'nnnn_
Cuts at: 3677 4164 4211 4232 4712 3677
Size: 487 47 21 480 7339
Fragments arranged by size:
7339 487 480 47 21
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2142 2218 2535 2568 3120 3209 3222
Size: 399 352 76 317 33 552 89 13
Cuts at: 3222 3255 3876 3909 4149 4549 4737 5003 5044
Size: 33 621 33 240 400 188 266 41
Cuts at: 5044 5138 5235 5333 5425 5693 6435 6520 7591
Size: 94 97 98 92 268 742 85 1071
Cuts at: 7591 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 621 552 400 399 352
317 268 266 240 188 98 97 94
92 89 85 76 41 33 33 33
13
BspEI T'CCGG_A
Cuts at: 3572 4405 5335 8037 3572
Size: 833 930 2702 3909
Fragments arranged by size:
3909 2702 930 833
BspHI T'CATG_A
Cuts at: 6263 7993 6263
Size: 1730 6644
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2805 3747 4894 5202 5640 5729 844
Size: 1961 942 1147 308 438 89 3489
Fragments arranged by size:
3489 1961 1147 942 438 308 89
BsrBI CCG'CTC
Cuts at: 1394 1823 2344 2946 3478 4813 4831 4864 5059
Size: 429 521 602 532 1335 18 33 195
Cuts at: 5059 6261 1394
Size: 1202 3507
Fragments arranged by size:
3507 1335 1202 602 532 521 429 195
33 18
BsrDI GCAATG_nn'
Cuts at: 65 1248 3898 4817 6869 7043 65
Size: 1183 2650 919 2052 174 1396
Fragments arranged by size:
2650 2052 1396 1183 919 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2710 4253 7018 7205 1700
Size: 110 900 1543 2765 187 2869
Fragments arranged by size:
2869 2765 1543 900 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8374
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8374
BssSI C'ACGA_G
Cuts at: 6434 7446 6434
Size: 1012 7362
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2206 5584 794
Size: 1412 3378 3584
Fragments arranged by size:
3584 3378 1412
BstBI TT'CG_AA
Cuts at: 2007 7178 2007
Size: 5171 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8374
BstXI CCAn_nnnn'nTGG
Cuts at: 4368 4368
Size: 8374
BstYI r'GATC_y
Cuts at: 1972 2182 2281 3084 5704 6071 6456 6473 7925
Size: 210 99 803 2620 367 385 17 1452
Cuts at: 7925 8011 8023 8369 1972
Size: 86 12 346 1977
Fragments arranged by size:
2620 1977 1452 803 385 367 346 210
99 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2463 4140 4641 2463
Size: 1677 501 6196
Fragments arranged by size:
6196 1677 501
BtgI C'CryG_G
Cuts at: 513 1065 2022 2128 2142 2406 2433 2619 3022
Size: 552 957 106 14 264 27 186 403
Cuts at: 3022 3068 3837 4875 5597 513
Size: 46 769 1038 722 3290
Fragments arranged by size:
3290 1038 957 769 722 552 403 264
186 106 46 27 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5108 487
Size: 683 56 3882 3753
Fragments arranged by size:
3882 3753 683 56
BtsI GCAGTG_nn'
Cuts at: 3108 4233 5585 5890 6684 6704 3108
Size: 1125 1352 305 794 20 4778
Fragments arranged by size:
4778 1352 1125 794 305 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8374
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8339
DraI TTT'AAA
Cuts at: 5678 5678
Size: 8374
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4298 7381 809
Size: 507 2982 3083 1802
Fragments arranged by size:
3083 2982 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2545 3025 3065
Size: 54 1425 50 275 18 715 480 40
Cuts at: 3065 4203 4420 4722 4814 4856 5529 5821 6710
Size: 1138 217 302 92 42 673 292 889
Cuts at: 6710 8339 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1138 889 715 673 480 302
292 275 217 92 54 50 43 42
40 18
EagI C'GGCC_G
Cuts at: 1537 1812 5821 1537
Size: 275 4009 4090
Fragments arranged by size:
4090 4009 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3870 4943 5334 6304 1353
Size: 2517 1073 391 970 3423
Fragments arranged by size:
3423 2517 1073 970 391
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2175 2679 2882 2891 3077 3748
Size: 1064 138 586 504 203 9 186 671
Cuts at: 3748 3963 3975 4281 6961 7335 7481 387
Size: 215 12 306 2680 374 146 1280
Fragments arranged by size:
2680 1280 1064 671 586 504 374 306
215 203 186 146 138 12 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2156 2240 2920 3116 3245 3620 3689
Size: 393 524 84 680 196 129 375 69
Cuts at: 3689 3938 4985 5058 5189 5270 5608 6437 7034
Size: 249 1047 73 131 81 338 829 597
Cuts at: 7034 7821 8089 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1047 829 787 680 597 524 393
375 338 268 249 196 131 129 84
81 73 69
EcoNI CCTnn'n_nnAGG
Cuts at: 5558 5558
Size: 8374
EcoO109I rG'GnC_Cy
Cuts at: 2051 2476 2532 3002 3078 3100 3116 4384 4645
Size: 425 56 470 76 22 16 1268 261
Cuts at: 4645 4999 5164 5551 2051
Size: 354 165 387 4874
Fragments arranged by size:
4874 1268 470 425 387 354 261 165
76 56 22 16
EcoRI G'AATT_C
Cuts at: 5683 6061 5683
Size: 378 7996
FspI TGC'GCA
Cuts at: 6880 6880
Size: 8374
HaeII r_GCGC'y
Cuts at: 1678 2196 2240 2295 2330 2493 2675 2809 2920
Size: 518 44 55 35 163 182 134 111
Cuts at: 2920 3022 3150 3442 3597 4492 5547 7521 1678
Size: 102 128 292 155 895 1055 1974 2531
Fragments arranged by size:
2531 1974 1055 895 518 292 182 163
155 134 128 111 102 55 44 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2167 2199 2918 2950 3281
Size: 32 135 32 595 32 719 32 331
Cuts at: 3281 3313 3377 3409 3604 3636 4260 4292 5012
Size: 32 64 32 195 32 624 32 720
Cuts at: 5012 5044 7015 7047 7089 7121 1373
Size: 32 1971 32 42 32 2626
Fragments arranged by size:
2626 1971 720 719 624 595 331 195
135 64 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2626 5718 5964 669
Size: 988 281 688 3092 246 3079
Fragments arranged by size:
3092 3079 988 688 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8374
HpaI GTT'AAC
Cuts at: 5964 5964
Size: 8374
KasI G'GCGC_C
Cuts at: 2192 2236 2291 2326 2671 2805 3018 3146 3593
Size: 44 55 35 345 134 213 128 447
Cuts at: 3593 5543 2192
Size: 1950 5023
Fragments arranged by size:
5023 1950 447 345 213 134 128 55
44 35
KpnI G_GTAC'C
Cuts at: 4486 5699 4486
Size: 1213 7161
MfeI C'AATT_G
Cuts at: 5973 5973
Size: 8374
MluI A'CGCG_T
Cuts at: 8250 8250
Size: 8374
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3195 7488 7672 1305
Size: 1890 4293 184 2007
Fragments arranged by size:
4293 2007 1890 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4205 5531 10
Size: 54 1425 343 2373 1326 2853
Fragments arranged by size:
2853 2373 1425 1326 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 6332 6691 6850 518
Size: 648 5166 359 159 2042
Fragments arranged by size:
5166 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2032 2144 2227 2435 2638 2940 3839 3913
Size: 426 112 83 208 203 302 899 74
Cuts at: 3913 4021 4060 4118 4842 5305 5661 5790 6467
Size: 108 39 58 724 463 356 129 677
Cuts at: 6467 7615 7860 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 899 724 677 463 426 356
302 245 208 203 129 112 108 83
74 58 39
NaeI GCC'GGC
Cuts at: 2712 4255 2712
Size: 1543 6831
NarI GG'CG_CC
Cuts at: 2193 2237 2292 2327 2672 2806 3019 3147 3594
Size: 44 55 35 345 134 213 128 447
Cuts at: 3594 5544 2193
Size: 1950 5023
Fragments arranged by size:
5023 1950 447 345 213 134 128 55
44 35
NcoI C'CATG_G
Cuts at: 513 1065 2022 2128 2406 513
Size: 552 957 106 278 6481
Fragments arranged by size:
6481 957 552 278 106
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8374
NgoMIV G'CCGG_C
Cuts at: 2710 4253 2710
Size: 1543 6831
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8374
NotI GC'GGCC_GC
Cuts at: 5821 5821
Size: 8374
NspI r_CATG'y
Cuts at: 1132 1229 5587 5732 7277 1132
Size: 97 4358 145 1545 2229
Fragments arranged by size:
4358 2229 1545 145 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8374
PciI A'CATG_T
Cuts at: 7273 7273
Size: 8374
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2134 5167 5527 1108
Size: 1026 3033 360 3955
Fragments arranged by size:
3955 3033 1026 360
PfoI T'CCnGG_A
Cuts at: 4281 4506 4281
Size: 225 8149
PmeI GTTT'AAAC
Cuts at: 5678 5678
Size: 8374
PmlI CAC'GTG
Cuts at: 4805 4805
Size: 8374
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2402 2434 2843 2875 6443 6475 7956 7988 2402
Size: 32 409 32 3568 32 1481 32 2788
Fragments arranged by size:
3568 2788 1481 409 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2476 3002 3100 5551 2476
Size: 526 98 2451 5299
Fragments arranged by size:
5299 2451 526 98
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8374
PsiI TTA'TAA
Cuts at: 5944 5944
Size: 8374
PspOMI G'GGCC_C
Cuts at: 2531 2564 3116 4999 2531
Size: 33 552 1883 5906
Fragments arranged by size:
5906 1883 552 33
PspXI vC'TCGA_Gb
Cuts at: 2108 3325 2108
Size: 1217 7157
PstI C_TGCA'G
Cuts at: 830 2106 3528 3610 3744 3847 4395 5280 5726
Size: 1276 1422 82 134 103 548 885 446
Cuts at: 5726 830
Size: 3478
Fragments arranged by size:
3478 1422 1276 885 548 446 134 103
82
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8374
PvuII CAG'CTG
Cuts at: 2032 2940 5305 2032
Size: 908 2365 5101
Fragments arranged by size:
5101 2365 908
SacII CC_GC'GG
Cuts at: 2145 2436 3840 2145
Size: 291 1404 6679
Fragments arranged by size:
6679 1404 291
SalI G'TCGA_C
Cuts at: 1936 5716 1936
Size: 3780 4594
SanDI GG'GwC_CC
Cuts at: 2476 2476
Size: 8374
SbfI CC_TGCA'GG
Cuts at: 3744 5280 5726 3744
Size: 1536 446 6392
Fragments arranged by size:
6392 1536 446
ScaI AGT'ACT
Cuts at: 6622 8334 6622
Size: 1712 6662
SexAI A'CCwGG_T
Cuts at: 2928 2928
Size: 8374
SfcI C'TryA_G
Cuts at: 826 1045 2102 3524 3606 3740 3843 4391 4986
Size: 219 1057 1422 82 134 103 548 595
Cuts at: 4986 5276 5722 6857 7538 7729 826
Size: 290 446 1135 681 191 1471
Fragments arranged by size:
1471 1422 1135 1057 681 595 548 446
290 219 191 134 103 82
SfiI GGCCn_nnn'nGGCC
Cuts at: 3064 3064
Size: 8374
SfoI GGC'GCC
Cuts at: 2194 2238 2293 2328 2673 2807 3020 3148 3595
Size: 44 55 35 345 134 213 128 447
Cuts at: 3595 5545 2194
Size: 1950 5023
Fragments arranged by size:
5023 1950 447 345 213 134 128 55
44 35
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8374
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8374
SmaI CCC'GGG
Cuts at: 1872 3161 5701 1872
Size: 1289 2540 4545
Fragments arranged by size:
4545 2540 1289
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2108 2777 3233 3325 3425 3680
Size: 197 926 165 669 456 92 100 255
Cuts at: 3680 4328 5402 6478 7379 7641 820
Size: 648 1074 1076 901 262 1553
Fragments arranged by size:
1553 1076 1074 926 901 669 648 456
262 255 197 165 100 92
SnaBI TAC'GTA
Cuts at: 493 5671 6165 493
Size: 5178 494 2702
Fragments arranged by size:
5178 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8374
SphI G_CATG'C
Cuts at: 1132 5587 5732 1132
Size: 4455 145 3774
Fragments arranged by size:
4455 3774 145
SspI AAT'ATT
Cuts at: 5 52 6298 5
Size: 47 6246 2081
Fragments arranged by size:
6246 2081 47
StuI AGG'CCT
Cuts at: 1517 3522 1517
Size: 2005 6369
StyI C'CwwG_G
Cuts at: 513 1065 2022 2128 2322 2406 2481 2724 3255
Size: 552 957 106 194 84 75 243 531
Cuts at: 3255 3828 4920 5813 513
Size: 573 1092 893 3074
Fragments arranged by size:
3074 1092 957 893 573 552 531 243
194 106 84 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2107 3007 3459 3949 4378 6401 6418
Size: 1255 527 900 452 490 429 2023 17
Cuts at: 6418 6571 6756 7201 325
Size: 153 185 445 1498
Fragments arranged by size:
2023 1498 1255 900 527 490 452 445
429 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6620 8332 96
Size: 274 80 33 51 1443 4643 1712 138
Fragments arranged by size:
4643 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3928 3960 8334 8366 3928
Size: 32 4374 32 3936
Fragments arranged by size:
4374 3936 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2930 3719 1316
Size: 1614 789 5971
Fragments arranged by size:
5971 1614 789
XbaI T'CTAG_A
Cuts at: 5710 5710
Size: 8374
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4450 4450
Size: 8374
XhoI C'TCGA_G
Cuts at: 1943 2108 3325 3680 1943
Size: 165 1217 355 6637
Fragments arranged by size:
6637 1217 355 165
XmaI C'CCGG_G
Cuts at: 1870 3159 5699 1870
Size: 1289 2540 4545
Fragments arranged by size:
4545 2540 1289
XmnI GAAnn'nnTTC
Cuts at: 6503 6503
Size: 8374
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6182 276
Size: 53 83 186 936 4648 2468
Fragments arranged by size:
4648 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SacII SalI SanDI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BfrBI BsiWI BstZ17I DraIII EcoICRI
EcoRV FalI FseI FspAI NruI NsiI PacI PsrI
RsrII SacI SapI SrfI SwaI