(Circular) (Six-base) MAPSORT of: pFN21ASDA1201.seq Check: 9496 from: 1 to: 6823 pFN21ASDA1201 6823 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3621 3759 3621 Size: 138 6685 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4633 278 Size: 53 83 186 936 3097 2468 Fragments arranged by size: 3097 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4166 1937 Size: 2229 4594 Acc65I G'GTAC_C Cuts at: 4144 4144 Size: 6823 AclI AA'CG_TT Cuts at: 1563 3443 4950 5323 1563 Size: 1880 1507 373 3063 Fragments arranged by size: 3063 1880 1507 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2179 2261 3034 4037 4886 6270 6538 1632 Size: 547 82 773 1003 849 1384 268 1917 Fragments arranged by size: 1917 1384 1003 849 773 547 268 82 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6626 AflIII A'CryG_T Cuts at: 1203 3691 4080 5722 6699 1203 Size: 2488 389 1642 977 1327 Fragments arranged by size: 2488 1642 1327 977 389 AgeI A'CCGG_T Cuts at: 5654 5654 Size: 6823 AhdI GACnn_n'nnGTC Cuts at: 3392 4081 5552 3392 Size: 689 1471 4663 Fragments arranged by size: 4663 1471 689 AleI CACnn'nnGTG Cuts at: 1166 3365 1166 Size: 2199 4624 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6823 AlwNI CAG_nnn'CTG Cuts at: 4566 6138 4566 Size: 1572 5251 ApaI G_GGCC'C Cuts at: 2467 3940 2467 Size: 1473 5350 ApaLI G'TGCA_C Cuts at: 2253 4880 6036 2253 Size: 2627 1156 3040 Fragments arranged by size: 3040 2627 1156 ApoI r'AATT_y Cuts at: 1493 2128 4132 4358 4510 6619 1493 Size: 635 2004 226 152 2109 1697 Fragments arranged by size: 2109 2004 1697 635 226 152 AseI AT'TA_AT Cuts at: 160 1032 5377 160 Size: 872 4345 1606 Fragments arranged by size: 4345 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6823 AvaI C'yCGr_G Cuts at: 1870 1943 2466 4148 1870 Size: 73 523 1682 4545 Fragments arranged by size: 4545 1682 523 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3947 3980 3947 Size: 33 6790 BamHI G'GATC_C Cuts at: 4153 4520 4153 Size: 367 6456 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4144 5599 618 Size: 325 217 627 2357 1455 1842 Fragments arranged by size: 2357 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2039 2467 2544 2859 2958 3647 3940 3981 Size: 648 428 77 315 99 689 293 41 Cuts at: 3981 4142 1391 Size: 161 4072 Fragments arranged by size: 4072 689 648 428 315 293 161 99 77 41 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2196 3034 928 Size: 971 297 838 4717 Fragments arranged by size: 4717 971 838 297 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5014 5048 1903 Size: 34 3077 34 3678 Fragments arranged by size: 3678 3077 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2633 4715 5925 2633 Size: 2082 1210 3531 Fragments arranged by size: 3531 2082 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6823 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2118 5434 136 Size: 107 122 71 631 605 446 3316 1525 Fragments arranged by size: 3316 1525 631 605 446 122 107 71 BglII A'GATC_T Cuts at: 6818 6818 Size: 6823 BlpI GC'TnA_GC Cuts at: 4240 4240 Size: 6823 Bme1580I G_kGCm'C Cuts at: 1790 2257 2467 2613 3321 3940 4884 6040 1790 Size: 467 210 146 708 619 944 1156 2573 Fragments arranged by size: 2573 1156 944 708 619 467 210 146 BmgBI CAC'GTC Cuts at: 1318 3316 4083 1318 Size: 1998 767 4058 Fragments arranged by size: 4058 1998 767 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2098 3913 4554 Size: 416 53 83 399 24 681 1815 641 Cuts at: 4554 5512 442 Size: 958 1753 Fragments arranged by size: 1815 1753 958 681 641 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6823 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2053 2288 2053 Size: 235 6588 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2834 3484 3494 5483 1239 Size: 1595 650 10 1989 2579 Fragments arranged by size: 2579 1989 1595 650 10 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4948 5813 6111 4948 Size: 865 298 5660 Fragments arranged by size: 5660 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2699 3400 5486 882 Size: 617 1200 701 2086 2219 Fragments arranged by size: 2219 2086 1200 701 617 BsaAI yAC'GTr Cuts at: 493 1204 2608 3031 3286 4120 4614 493 Size: 711 1404 423 255 834 494 2702 Fragments arranged by size: 2702 1404 834 711 494 423 255 BsaBI GATnn'nnATC Cuts at: 6817 6817 Size: 6823 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4630 5012 5647 275 Size: 53 83 186 936 3097 382 635 1451 Fragments arranged by size: 3097 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2071 5256 5654 5928 6075 6486 2071 Size: 3185 398 274 147 411 2408 Fragments arranged by size: 3185 2408 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3583 3613 1178 Size: 30 165 30 2180 30 4388 Fragments arranged by size: 4388 2180 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2038 2089 3065 3173 3604 1186 Size: 852 51 976 108 431 4405 Fragments arranged by size: 4405 976 852 431 108 51 BseYI C'CCAG_C Cuts at: 2334 3939 6026 6522 2334 Size: 1605 2087 496 2635 Fragments arranged by size: 2635 2087 1605 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2105 1163 Size: 573 369 5881 Fragments arranged by size: 5881 573 369 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2113 4273 5034 6062 1540 Size: 275 186 112 2160 761 1028 2301 Fragments arranged by size: 2301 2160 1028 761 275 186 112 BsiHKAI G_wGCw'C Cuts at: 2257 2544 2859 2958 3524 3981 4884 4969 6040 Size: 287 315 99 566 457 903 85 1071 Cuts at: 6040 2257 Size: 3040 Fragments arranged by size: 3040 1071 903 566 457 315 287 99 85 BsmI GAATG_Cn' Cuts at: 2512 4334 4427 2512 Size: 1822 93 4908 Fragments arranged by size: 4908 1822 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2797 2829 2797 Size: 32 6791 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2039 2257 2467 2544 2613 2859 2958 Size: 399 249 218 210 77 69 246 99 Cuts at: 2958 3321 3524 3647 3940 3981 4142 4884 4969 Size: 363 203 123 293 41 161 742 85 Cuts at: 4969 6040 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 363 293 249 246 218 210 203 161 123 99 85 77 69 41 BspEI T'CCGG_A Cuts at: 6486 6486 Size: 6823 BspHI T'CATG_A Cuts at: 4712 6442 4712 Size: 1730 5093 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3621 3689 3759 4178 844 Size: 2777 68 70 419 3489 Fragments arranged by size: 3489 2777 419 70 68 BsrBI CCG'CTC Cuts at: 1394 1823 2118 4710 1394 Size: 429 295 2592 3507 Fragments arranged by size: 3507 2592 429 295 BsrDI GCAATG_nn' Cuts at: 65 1248 5318 5492 65 Size: 1183 4070 174 1396 Fragments arranged by size: 4070 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3021 5467 5654 1700 Size: 110 1211 2446 187 2869 Fragments arranged by size: 2869 2446 1211 187 110 BsrGI T'GTAC_A Cuts at: 96 3255 96 Size: 3159 3664 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6823 BssSI C'ACGA_G Cuts at: 4883 5895 4883 Size: 1012 5811 BstAPI GCAn_nnn'nTGC Cuts at: 794 2083 794 Size: 1289 5534 BstBI TT'CG_AA Cuts at: 2007 5627 2007 Size: 3620 3203 BstEII G'GTnAC_C Cuts at: 1184 3649 3912 1184 Size: 2465 263 4095 Fragments arranged by size: 4095 2465 263 BstXI CCAn_nnnn'nTGG Cuts at: 2567 3214 2567 Size: 647 6176 BstYI r'GATC_y Cuts at: 1972 2307 2652 4153 4520 4905 4922 6374 6460 Size: 335 345 1501 367 385 17 1452 86 Cuts at: 6460 6472 6818 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1501 1452 385 367 346 345 335 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 3146 513 Size: 552 957 1124 4190 Fragments arranged by size: 4190 1124 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6084 Fragments arranged by size: 6084 683 56 BtsI GCAGTG_nn' Cuts at: 2785 3879 4339 5133 5153 2785 Size: 1094 460 794 20 4455 Fragments arranged by size: 4455 1094 794 460 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6823 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6788 DraI TTT'AAA Cuts at: 4127 4127 Size: 6823 DraIII CAC_nnn'GTG Cuts at: 3316 3316 Size: 6823 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5830 809 Size: 507 4514 1802 Fragments arranged by size: 4514 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2110 3019 3507 Size: 54 1425 50 275 18 280 909 488 Cuts at: 3507 4270 5159 6788 8 Size: 763 889 1629 43 Fragments arranged by size: 1629 1425 909 889 763 488 280 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2110 4270 1537 Size: 275 298 2160 4090 Fragments arranged by size: 4090 2160 298 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2643 2658 2814 2971 3083 3627 3842 4753 Size: 1290 15 156 157 112 544 215 911 Cuts at: 4753 1353 Size: 3423 Fragments arranged by size: 3423 1290 911 544 215 157 156 112 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3476 5410 5784 5930 387 Size: 1064 138 1887 1934 374 146 1280 Fragments arranged by size: 1934 1887 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2542 2857 2956 3979 2542 Size: 315 99 1023 5386 Fragments arranged by size: 5386 1023 315 99 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2179 2261 2834 3034 3484 3494 4037 Size: 393 547 82 573 200 450 10 543 Cuts at: 4037 4886 5483 6270 6538 1239 Size: 849 597 787 268 1524 Fragments arranged by size: 1524 849 787 597 573 547 543 450 393 268 200 82 10 EcoNI CCTnn'n_nnAGG Cuts at: 2849 3632 2849 Size: 783 6040 EcoO109I rG'GnC_Cy Cuts at: 2463 2785 2841 3010 4068 2463 Size: 322 56 169 1058 5218 Fragments arranged by size: 5218 1058 322 169 56 EcoRI G'AATT_C Cuts at: 2128 4132 4510 2128 Size: 2004 378 4441 Fragments arranged by size: 4441 2004 378 EcoRV GAT'ATC Cuts at: 3133 3133 Size: 6823 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2464 2464 Size: 6823 FspI TGC'GCA Cuts at: 5329 5329 Size: 6823 HaeII r_GCGC'y Cuts at: 1678 2233 2828 3124 5970 1678 Size: 555 595 296 2846 2531 Fragments arranged by size: 2846 2531 595 555 296 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2033 2065 2277 2309 2672 Size: 32 135 32 461 32 212 32 363 Cuts at: 2672 2704 2884 2916 3378 3410 3583 3615 4067 Size: 32 180 32 462 32 173 32 452 Cuts at: 4067 4099 5464 5496 5538 5570 1373 Size: 32 1365 32 42 32 2626 Fragments arranged by size: 2626 1365 462 461 452 363 212 180 173 135 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4167 4413 669 Size: 988 281 2229 246 3079 Fragments arranged by size: 3079 2229 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6823 HpaI GTT'AAC Cuts at: 4413 4413 Size: 6823 KpnI G_GTAC'C Cuts at: 4148 4148 Size: 6823 MfeI C'AATT_G Cuts at: 4422 4422 Size: 6823 MluI A'CGCG_T Cuts at: 6699 6699 Size: 6823 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2994 3837 4065 5937 6121 1305 Size: 1689 843 228 1872 184 2007 Fragments arranged by size: 2007 1872 1689 843 228 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3509 10 Size: 54 1425 343 1677 3324 Fragments arranged by size: 3324 1677 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3365 4781 5140 5299 518 Size: 648 2199 1416 359 159 2042 Fragments arranged by size: 2199 2042 1416 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2094 2334 2731 3100 3118 3786 4239 4916 Size: 488 240 397 369 18 668 453 677 Cuts at: 4916 6064 6309 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 668 488 453 397 369 245 240 18 NaeI GCC'GGC Cuts at: 3023 3023 Size: 6823 NcoI C'CATG_G Cuts at: 513 1065 2022 3146 513 Size: 552 957 1124 4190 Fragments arranged by size: 4190 1124 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6823 NgoMIV G'CCGG_C Cuts at: 3021 3021 Size: 6823 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6823 NotI GC'GGCC_GC Cuts at: 4270 4270 Size: 6823 NspI r_CATG'y Cuts at: 1132 1229 3695 4181 5726 1132 Size: 97 2466 486 1545 2229 Fragments arranged by size: 2466 2229 1545 486 97 PasI CC'CwG_GG Cuts at: 1348 3426 1348 Size: 2078 4745 PciI A'CATG_T Cuts at: 3691 5722 3691 Size: 2031 4792 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6823 PfoI T'CCnGG_A Cuts at: 3723 3723 Size: 6823 PmeI GTTT'AAAC Cuts at: 4127 4127 Size: 6823 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4892 4924 6405 6437 4892 Size: 32 1481 32 5278 Fragments arranged by size: 5278 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2841 3010 4068 2841 Size: 169 1058 5596 Fragments arranged by size: 5596 1058 169 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6823 PsiI TTA'TAA Cuts at: 4393 4393 Size: 6823 PspOMI G'GGCC_C Cuts at: 2463 3936 2463 Size: 1473 5350 PstI C_TGCA'G Cuts at: 830 3756 4175 830 Size: 2926 419 3478 Fragments arranged by size: 3478 2926 419 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6823 PvuII CAG'CTG Cuts at: 2094 2731 3786 2094 Size: 637 1055 5131 Fragments arranged by size: 5131 1055 637 SacI G_AGCT'C Cuts at: 2544 2859 2958 3981 2544 Size: 315 99 1023 5386 Fragments arranged by size: 5386 1023 315 99 SalI G'TCGA_C Cuts at: 1936 4165 1936 Size: 2229 4594 SanDI GG'GwC_CC Cuts at: 2841 2841 Size: 6823 SapI GCTCTTCn'nnn_ Cuts at: 3842 3842 Size: 6823 SbfI CC_TGCA'GG Cuts at: 4175 4175 Size: 6823 ScaI AGT'ACT Cuts at: 5071 6783 5071 Size: 1712 5111 SfcI C'TryA_G Cuts at: 826 1045 2317 3752 4171 5306 5987 6178 826 Size: 219 1272 1435 419 1135 681 191 1471 Fragments arranged by size: 1471 1435 1272 1135 681 419 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6823 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6823 SmaI CCC'GGG Cuts at: 1872 2468 4150 1872 Size: 596 1682 4545 Fragments arranged by size: 4545 1682 596 SmlI C'TyrA_G Cuts at: 820 1017 1943 4927 5828 6090 820 Size: 197 926 2984 901 262 1553 Fragments arranged by size: 2984 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4120 4614 493 Size: 3627 494 2702 Fragments arranged by size: 3627 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6823 SphI G_CATG'C Cuts at: 1132 4181 1132 Size: 3049 3774 SspI AAT'ATT Cuts at: 5 52 4747 5 Size: 47 4695 2081 Fragments arranged by size: 4695 2081 47 StuI AGG'CCT Cuts at: 1517 2999 1517 Size: 1482 5341 StyI C'CwwG_G Cuts at: 513 1065 2022 2535 3146 4262 513 Size: 552 957 513 611 1116 3074 Fragments arranged by size: 3074 1116 957 611 552 513 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3323 4850 4867 5020 5205 5650 325 Size: 1255 1743 1527 17 153 185 445 1498 Fragments arranged by size: 1743 1527 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3255 5069 6781 Size: 274 80 33 51 1443 1278 1814 1712 Cuts at: 6781 96 Size: 138 Fragments arranged by size: 1814 1712 1443 1278 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6783 6815 6783 Size: 32 6791 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6823 XbaI T'CTAG_A Cuts at: 4159 4159 Size: 6823 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3772 3772 Size: 6823 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6823 XmaI C'CCGG_G Cuts at: 1870 2466 4148 1870 Size: 596 1682 4545 Fragments arranged by size: 4545 1682 596 XmnI GAAnn'nnTTC Cuts at: 3443 3458 4952 3443 Size: 15 1494 5314 Fragments arranged by size: 5314 1494 15 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4631 276 Size: 53 83 186 936 3097 2468 Fragments arranged by size: 3097 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI