Restriction map for
pFN21ASDA1201
(Circular) (Six-base) MAPSORT of: pFN21ASDA1201.seq Check: 9496 from: 1 to: 6823
pFN21ASDA1201 6823 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3621 3759 3621
Size: 138 6685
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4633 278
Size: 53 83 186 936 3097 2468
Fragments arranged by size:
3097 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4166 1937
Size: 2229 4594
Acc65I G'GTAC_C
Cuts at: 4144 4144
Size: 6823
AclI AA'CG_TT
Cuts at: 1563 3443 4950 5323 1563
Size: 1880 1507 373 3063
Fragments arranged by size:
3063 1880 1507 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2179 2261 3034 4037 4886 6270 6538 1632
Size: 547 82 773 1003 849 1384 268 1917
Fragments arranged by size:
1917 1384 1003 849 773 547 268 82
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6626
AflIII A'CryG_T
Cuts at: 1203 3691 4080 5722 6699 1203
Size: 2488 389 1642 977 1327
Fragments arranged by size:
2488 1642 1327 977 389
AgeI A'CCGG_T
Cuts at: 5654 5654
Size: 6823
AhdI GACnn_n'nnGTC
Cuts at: 3392 4081 5552 3392
Size: 689 1471 4663
Fragments arranged by size:
4663 1471 689
AleI CACnn'nnGTG
Cuts at: 1166 3365 1166
Size: 2199 4624
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6823
AlwNI CAG_nnn'CTG
Cuts at: 4566 6138 4566
Size: 1572 5251
ApaI G_GGCC'C
Cuts at: 2467 3940 2467
Size: 1473 5350
ApaLI G'TGCA_C
Cuts at: 2253 4880 6036 2253
Size: 2627 1156 3040
Fragments arranged by size:
3040 2627 1156
ApoI r'AATT_y
Cuts at: 1493 2128 4132 4358 4510 6619 1493
Size: 635 2004 226 152 2109 1697
Fragments arranged by size:
2109 2004 1697 635 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5377 160
Size: 872 4345 1606
Fragments arranged by size:
4345 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6823
AvaI C'yCGr_G
Cuts at: 1870 1943 2466 4148 1870
Size: 73 523 1682 4545
Fragments arranged by size:
4545 1682 523 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3947 3980 3947
Size: 33 6790
BamHI G'GATC_C
Cuts at: 4153 4520 4153
Size: 367 6456
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4144 5599 618
Size: 325 217 627 2357 1455 1842
Fragments arranged by size:
2357 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2039 2467 2544 2859 2958 3647 3940 3981
Size: 648 428 77 315 99 689 293 41
Cuts at: 3981 4142 1391
Size: 161 4072
Fragments arranged by size:
4072 689 648 428 315 293 161 99
77 41
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2196 3034 928
Size: 971 297 838 4717
Fragments arranged by size:
4717 971 838 297
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5014 5048 1903
Size: 34 3077 34 3678
Fragments arranged by size:
3678 3077 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2633 4715 5925 2633
Size: 2082 1210 3531
Fragments arranged by size:
3531 2082 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6823
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2118 5434 136
Size: 107 122 71 631 605 446 3316 1525
Fragments arranged by size:
3316 1525 631 605 446 122 107 71
BglII A'GATC_T
Cuts at: 6818 6818
Size: 6823
BlpI GC'TnA_GC
Cuts at: 4240 4240
Size: 6823
Bme1580I G_kGCm'C
Cuts at: 1790 2257 2467 2613 3321 3940 4884 6040 1790
Size: 467 210 146 708 619 944 1156 2573
Fragments arranged by size:
2573 1156 944 708 619 467 210 146
BmgBI CAC'GTC
Cuts at: 1318 3316 4083 1318
Size: 1998 767 4058
Fragments arranged by size:
4058 1998 767
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2098 3913 4554
Size: 416 53 83 399 24 681 1815 641
Cuts at: 4554 5512 442
Size: 958 1753
Fragments arranged by size:
1815 1753 958 681 641 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6823
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2053 2288 2053
Size: 235 6588
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2834 3484 3494 5483 1239
Size: 1595 650 10 1989 2579
Fragments arranged by size:
2579 1989 1595 650 10
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4948 5813 6111 4948
Size: 865 298 5660
Fragments arranged by size:
5660 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2699 3400 5486 882
Size: 617 1200 701 2086 2219
Fragments arranged by size:
2219 2086 1200 701 617
BsaAI yAC'GTr
Cuts at: 493 1204 2608 3031 3286 4120 4614 493
Size: 711 1404 423 255 834 494 2702
Fragments arranged by size:
2702 1404 834 711 494 423 255
BsaBI GATnn'nnATC
Cuts at: 6817 6817
Size: 6823
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4630 5012 5647 275
Size: 53 83 186 936 3097 382 635 1451
Fragments arranged by size:
3097 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2071 5256 5654 5928 6075 6486 2071
Size: 3185 398 274 147 411 2408
Fragments arranged by size:
3185 2408 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3583 3613 1178
Size: 30 165 30 2180 30 4388
Fragments arranged by size:
4388 2180 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2038 2089 3065 3173 3604 1186
Size: 852 51 976 108 431 4405
Fragments arranged by size:
4405 976 852 431 108 51
BseYI C'CCAG_C
Cuts at: 2334 3939 6026 6522 2334
Size: 1605 2087 496 2635
Fragments arranged by size:
2635 2087 1605 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2105 1163
Size: 573 369 5881
Fragments arranged by size:
5881 573 369
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2113 4273 5034 6062 1540
Size: 275 186 112 2160 761 1028 2301
Fragments arranged by size:
2301 2160 1028 761 275 186 112
BsiHKAI G_wGCw'C
Cuts at: 2257 2544 2859 2958 3524 3981 4884 4969 6040
Size: 287 315 99 566 457 903 85 1071
Cuts at: 6040 2257
Size: 3040
Fragments arranged by size:
3040 1071 903 566 457 315 287 99
85
BsmI GAATG_Cn'
Cuts at: 2512 4334 4427 2512
Size: 1822 93 4908
Fragments arranged by size:
4908 1822 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2797 2829 2797
Size: 32 6791
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2039 2257 2467 2544 2613 2859 2958
Size: 399 249 218 210 77 69 246 99
Cuts at: 2958 3321 3524 3647 3940 3981 4142 4884 4969
Size: 363 203 123 293 41 161 742 85
Cuts at: 4969 6040 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 363 293 249 246
218 210 203 161 123 99 85 77
69 41
BspEI T'CCGG_A
Cuts at: 6486 6486
Size: 6823
BspHI T'CATG_A
Cuts at: 4712 6442 4712
Size: 1730 5093
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3621 3689 3759 4178 844
Size: 2777 68 70 419 3489
Fragments arranged by size:
3489 2777 419 70 68
BsrBI CCG'CTC
Cuts at: 1394 1823 2118 4710 1394
Size: 429 295 2592 3507
Fragments arranged by size:
3507 2592 429 295
BsrDI GCAATG_nn'
Cuts at: 65 1248 5318 5492 65
Size: 1183 4070 174 1396
Fragments arranged by size:
4070 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3021 5467 5654 1700
Size: 110 1211 2446 187 2869
Fragments arranged by size:
2869 2446 1211 187 110
BsrGI T'GTAC_A
Cuts at: 96 3255 96
Size: 3159 3664
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6823
BssSI C'ACGA_G
Cuts at: 4883 5895 4883
Size: 1012 5811
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2083 794
Size: 1289 5534
BstBI TT'CG_AA
Cuts at: 2007 5627 2007
Size: 3620 3203
BstEII G'GTnAC_C
Cuts at: 1184 3649 3912 1184
Size: 2465 263 4095
Fragments arranged by size:
4095 2465 263
BstXI CCAn_nnnn'nTGG
Cuts at: 2567 3214 2567
Size: 647 6176
BstYI r'GATC_y
Cuts at: 1972 2307 2652 4153 4520 4905 4922 6374 6460
Size: 335 345 1501 367 385 17 1452 86
Cuts at: 6460 6472 6818 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1501 1452 385 367 346 345 335
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 3146 513
Size: 552 957 1124 4190
Fragments arranged by size:
4190 1124 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6084
Fragments arranged by size:
6084 683 56
BtsI GCAGTG_nn'
Cuts at: 2785 3879 4339 5133 5153 2785
Size: 1094 460 794 20 4455
Fragments arranged by size:
4455 1094 794 460 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6823
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6788
DraI TTT'AAA
Cuts at: 4127 4127
Size: 6823
DraIII CAC_nnn'GTG
Cuts at: 3316 3316
Size: 6823
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5830 809
Size: 507 4514 1802
Fragments arranged by size:
4514 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2110 3019 3507
Size: 54 1425 50 275 18 280 909 488
Cuts at: 3507 4270 5159 6788 8
Size: 763 889 1629 43
Fragments arranged by size:
1629 1425 909 889 763 488 280 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2110 4270 1537
Size: 275 298 2160 4090
Fragments arranged by size:
4090 2160 298 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2643 2658 2814 2971 3083 3627 3842 4753
Size: 1290 15 156 157 112 544 215 911
Cuts at: 4753 1353
Size: 3423
Fragments arranged by size:
3423 1290 911 544 215 157 156 112
15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3476 5410 5784 5930 387
Size: 1064 138 1887 1934 374 146 1280
Fragments arranged by size:
1934 1887 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2542 2857 2956 3979 2542
Size: 315 99 1023 5386
Fragments arranged by size:
5386 1023 315 99
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2179 2261 2834 3034 3484 3494 4037
Size: 393 547 82 573 200 450 10 543
Cuts at: 4037 4886 5483 6270 6538 1239
Size: 849 597 787 268 1524
Fragments arranged by size:
1524 849 787 597 573 547 543 450
393 268 200 82 10
EcoNI CCTnn'n_nnAGG
Cuts at: 2849 3632 2849
Size: 783 6040
EcoO109I rG'GnC_Cy
Cuts at: 2463 2785 2841 3010 4068 2463
Size: 322 56 169 1058 5218
Fragments arranged by size:
5218 1058 322 169 56
EcoRI G'AATT_C
Cuts at: 2128 4132 4510 2128
Size: 2004 378 4441
Fragments arranged by size:
4441 2004 378
EcoRV GAT'ATC
Cuts at: 3133 3133
Size: 6823
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2464 2464
Size: 6823
FspI TGC'GCA
Cuts at: 5329 5329
Size: 6823
HaeII r_GCGC'y
Cuts at: 1678 2233 2828 3124 5970 1678
Size: 555 595 296 2846 2531
Fragments arranged by size:
2846 2531 595 555 296
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2033 2065 2277 2309 2672
Size: 32 135 32 461 32 212 32 363
Cuts at: 2672 2704 2884 2916 3378 3410 3583 3615 4067
Size: 32 180 32 462 32 173 32 452
Cuts at: 4067 4099 5464 5496 5538 5570 1373
Size: 32 1365 32 42 32 2626
Fragments arranged by size:
2626 1365 462 461 452 363 212 180
173 135 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4167 4413 669
Size: 988 281 2229 246 3079
Fragments arranged by size:
3079 2229 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6823
HpaI GTT'AAC
Cuts at: 4413 4413
Size: 6823
KpnI G_GTAC'C
Cuts at: 4148 4148
Size: 6823
MfeI C'AATT_G
Cuts at: 4422 4422
Size: 6823
MluI A'CGCG_T
Cuts at: 6699 6699
Size: 6823
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2994 3837 4065 5937 6121 1305
Size: 1689 843 228 1872 184 2007
Fragments arranged by size:
2007 1872 1689 843 228 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3509 10
Size: 54 1425 343 1677 3324
Fragments arranged by size:
3324 1677 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3365 4781 5140 5299 518
Size: 648 2199 1416 359 159 2042
Fragments arranged by size:
2199 2042 1416 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2094 2334 2731 3100 3118 3786 4239 4916
Size: 488 240 397 369 18 668 453 677
Cuts at: 4916 6064 6309 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 668 488 453 397 369
245 240 18
NaeI GCC'GGC
Cuts at: 3023 3023
Size: 6823
NcoI C'CATG_G
Cuts at: 513 1065 2022 3146 513
Size: 552 957 1124 4190
Fragments arranged by size:
4190 1124 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6823
NgoMIV G'CCGG_C
Cuts at: 3021 3021
Size: 6823
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6823
NotI GC'GGCC_GC
Cuts at: 4270 4270
Size: 6823
NspI r_CATG'y
Cuts at: 1132 1229 3695 4181 5726 1132
Size: 97 2466 486 1545 2229
Fragments arranged by size:
2466 2229 1545 486 97
PasI CC'CwG_GG
Cuts at: 1348 3426 1348
Size: 2078 4745
PciI A'CATG_T
Cuts at: 3691 5722 3691
Size: 2031 4792
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6823
PfoI T'CCnGG_A
Cuts at: 3723 3723
Size: 6823
PmeI GTTT'AAAC
Cuts at: 4127 4127
Size: 6823
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4892 4924 6405 6437 4892
Size: 32 1481 32 5278
Fragments arranged by size:
5278 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2841 3010 4068 2841
Size: 169 1058 5596
Fragments arranged by size:
5596 1058 169
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6823
PsiI TTA'TAA
Cuts at: 4393 4393
Size: 6823
PspOMI G'GGCC_C
Cuts at: 2463 3936 2463
Size: 1473 5350
PstI C_TGCA'G
Cuts at: 830 3756 4175 830
Size: 2926 419 3478
Fragments arranged by size:
3478 2926 419
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6823
PvuII CAG'CTG
Cuts at: 2094 2731 3786 2094
Size: 637 1055 5131
Fragments arranged by size:
5131 1055 637
SacI G_AGCT'C
Cuts at: 2544 2859 2958 3981 2544
Size: 315 99 1023 5386
Fragments arranged by size:
5386 1023 315 99
SalI G'TCGA_C
Cuts at: 1936 4165 1936
Size: 2229 4594
SanDI GG'GwC_CC
Cuts at: 2841 2841
Size: 6823
SapI GCTCTTCn'nnn_
Cuts at: 3842 3842
Size: 6823
SbfI CC_TGCA'GG
Cuts at: 4175 4175
Size: 6823
ScaI AGT'ACT
Cuts at: 5071 6783 5071
Size: 1712 5111
SfcI C'TryA_G
Cuts at: 826 1045 2317 3752 4171 5306 5987 6178 826
Size: 219 1272 1435 419 1135 681 191 1471
Fragments arranged by size:
1471 1435 1272 1135 681 419 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6823
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6823
SmaI CCC'GGG
Cuts at: 1872 2468 4150 1872
Size: 596 1682 4545
Fragments arranged by size:
4545 1682 596
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4927 5828 6090 820
Size: 197 926 2984 901 262 1553
Fragments arranged by size:
2984 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4120 4614 493
Size: 3627 494 2702
Fragments arranged by size:
3627 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6823
SphI G_CATG'C
Cuts at: 1132 4181 1132
Size: 3049 3774
SspI AAT'ATT
Cuts at: 5 52 4747 5
Size: 47 4695 2081
Fragments arranged by size:
4695 2081 47
StuI AGG'CCT
Cuts at: 1517 2999 1517
Size: 1482 5341
StyI C'CwwG_G
Cuts at: 513 1065 2022 2535 3146 4262 513
Size: 552 957 513 611 1116 3074
Fragments arranged by size:
3074 1116 957 611 552 513
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3323 4850 4867 5020 5205 5650 325
Size: 1255 1743 1527 17 153 185 445 1498
Fragments arranged by size:
1743 1527 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3255 5069 6781
Size: 274 80 33 51 1443 1278 1814 1712
Cuts at: 6781 96
Size: 138
Fragments arranged by size:
1814 1712 1443 1278 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6783 6815 6783
Size: 32 6791
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6823
XbaI T'CTAG_A
Cuts at: 4159 4159
Size: 6823
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3772 3772
Size: 6823
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6823
XmaI C'CCGG_G
Cuts at: 1870 2466 4148 1870
Size: 596 1682 4545
Fragments arranged by size:
4545 1682 596
XmnI GAAnn'nnTTC
Cuts at: 3443 3458 4952 3443
Size: 15 1494 5314
Fragments arranged by size:
5314 1494 15
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4631 276
Size: 53 83 186 936 3097 2468
Fragments arranged by size:
3097 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BbvCI BfrBI Bpu10I
BsiWI BstZ17I Bsu36I FseI FspAI KasI NarI NruI
NsiI PacI PmlI PspXI PsrI RsrII SacII SexAI
SfiI SfoI SrfI SwaI