Restriction map for
pFN21ASDA1200
(Circular) (Six-base) MAPSORT of: pFN21ASDA1200.seq Check: 7973 from: 1 to: 8821
pFN21ASDA1200 8821 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2488 4397 2488
Size: 1909 6912
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6631 278
Size: 53 83 186 936 5095 2468
Fragments arranged by size:
5095 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2058 3414 3438 6164 1937
Size: 121 1356 24 2726 4594
Fragments arranged by size:
4594 2726 1356 121 24
Acc65I G'GTAC_C
Cuts at: 6142 6142
Size: 8821
AclI AA'CG_TT
Cuts at: 1563 5846 6107 6948 7321 1563
Size: 4283 261 841 373 3063
Fragments arranged by size:
4283 3063 841 373 261
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2270 2583 2675 2733 2830 3470 4055 4996
Size: 638 313 92 58 97 640 585 941
Cuts at: 4996 5820 6884 8268 8536 1632
Size: 824 1064 1384 268 1917
Fragments arranged by size:
1917 1384 1064 941 824 640 638 585
313 268 97 92 58
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8624
AflIII A'CryG_T
Cuts at: 1203 5278 7720 8697 1203
Size: 4075 2442 977 1327
Fragments arranged by size:
4075 2442 1327 977
AgeI A'CCGG_T
Cuts at: 7652 7652
Size: 8821
AhdI GACnn_n'nnGTC
Cuts at: 7550 7550
Size: 8821
AleI CACnn'nnGTG
Cuts at: 1166 4313 1166
Size: 3147 5674
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5039 1184
Size: 3855 4966
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5340 5372 5340
Size: 32 8789
AlwNI CAG_nnn'CTG
Cuts at: 2158 2170 2467 2488 2653 3692 4030 4403 5098
Size: 12 297 21 165 1039 338 373 695
Cuts at: 5098 5489 6564 8136 2158
Size: 391 1075 1572 2843
Fragments arranged by size:
2843 1572 1075 1039 695 391 373 338
297 165 21 12
ApaI G_GGCC'C
Cuts at: 5575 5575
Size: 8821
ApaLI G'TGCA_C
Cuts at: 5024 5813 6878 8034 5024
Size: 789 1065 1156 5811
Fragments arranged by size:
5811 1156 1065 789
ApoI r'AATT_y
Cuts at: 1493 6130 6356 6508 8617 1493
Size: 4637 226 152 2109 1697
Fragments arranged by size:
4637 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7375 160
Size: 872 6343 1606
Fragments arranged by size:
6343 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8821
AvaI C'yCGr_G
Cuts at: 1870 1943 3120 3132 3591 3859 4694 5164 6146
Size: 73 1177 12 459 268 835 470 982
Cuts at: 6146 1870
Size: 4545
Fragments arranged by size:
4545 1177 982 835 470 459 268 73
12
BamHI G'GATC_C
Cuts at: 2504 4011 6151 6518 2504
Size: 1507 2140 367 4807
Fragments arranged by size:
4807 2140 1507 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2044 2560 2684 2978 3012
Size: 325 217 627 257 516 124 294 34
Cuts at: 3012 4076 4358 4500 5592 6142 7597 618
Size: 1064 282 142 1092 550 1455 1842
Fragments arranged by size:
1842 1455 1092 1064 627 550 516 325
294 282 257 217 142 124 34
BanII G_rGCy'C
Cuts at: 1391 2328 2813 2974 3203 3632 5575 5906 6140
Size: 937 485 161 229 429 1943 331 234
Cuts at: 6140 1391
Size: 4072
Fragments arranged by size:
4072 1943 937 485 429 331 234 229
161
BbeI G_GCGC'C
Cuts at: 2048 2048
Size: 8821
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3575 3806 5727 928
Size: 971 1676 231 1921 4022
Fragments arranged by size:
4022 1921 1676 971 231
BbvCI CC'TCA_GC
Cuts at: 4815 5588 4815
Size: 773 8048
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7012 7046 1903
Size: 34 5075 34 3678
Fragments arranged by size:
5075 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4369 6713 7923 4369
Size: 2344 1210 5267
Fragments arranged by size:
5267 2344 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8821
BfrBI ATG'CAT
Cuts at: 2829 2829
Size: 8821
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5441 5493 7432
Size: 107 122 71 631 605 3769 52 1939
Cuts at: 7432 136
Size: 1525
Fragments arranged by size:
3769 1939 1525 631 605 122 107 71
52
BglII A'GATC_T
Cuts at: 4710 5205 5885 8816 4710
Size: 495 680 2931 4715
Fragments arranged by size:
4715 2931 680 495
BlpI GC'TnA_GC
Cuts at: 2346 3219 5514 5718 6238 2346
Size: 873 2295 204 520 4929
Fragments arranged by size:
4929 2295 873 520 204
Bme1580I G_kGCm'C
Cuts at: 1790 2565 2689 3045 4433 5028 5575 5817 6882
Size: 775 124 356 1388 595 547 242 1065
Cuts at: 6882 8038 1790
Size: 1156 2573
Fragments arranged by size:
2573 1388 1156 1065 775 595 547 356
242 124
BmgBI CAC'GTC
Cuts at: 1318 3804 1318
Size: 2486 6335
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 6552 7510 442
Size: 416 53 83 399 24 5135 958 1753
Fragments arranged by size:
5135 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8821
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2918 3097 2918
Size: 179 8642
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2180 2195 2372 3134 3245 3779 4007 4022
Size: 941 15 177 762 111 534 228 15
Cuts at: 4022 4041 4296 4516 5075 5198 5510 5511 7481
Size: 19 255 220 559 123 312 1 1970
Cuts at: 7481 1239
Size: 2579
Fragments arranged by size:
2579 1970 941 762 559 534 312 255
228 220 177 123 111 19 15 15
1
Bpu10I CC'TnA_GC
Cuts at: 3108 3693 4815 5588 3108
Size: 585 1122 773 6341
Fragments arranged by size:
6341 1122 773 585
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2018 2417 2468 3867 4568 5444 6946 7811 8109
Size: 399 51 1399 701 876 1502 865 298
Cuts at: 8109 2018
Size: 2730
Fragments arranged by size:
2730 1502 1399 876 865 701 399 298
51
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2198 7484 882
Size: 617 699 5286 2219
Fragments arranged by size:
5286 2219 699 617
BsaAI yAC'GTr
Cuts at: 493 1204 4380 6118 6612 493
Size: 711 3176 1738 494 2702
Fragments arranged by size:
3176 2702 1738 711 494
BsaBI GATnn'nnATC
Cuts at: 8815 8815
Size: 8821
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2045 3609 3660 5781
Size: 53 83 186 936 512 1564 51 2121
Cuts at: 5781 6628 7010 7645 275
Size: 847 382 635 1451
Fragments arranged by size:
2121 1564 1451 936 847 635 512 382
186 83 53 51
BsaWI w'CCGG_w
Cuts at: 2100 2280 3873 5937 7254 7652 7926 8073 8484
Size: 180 1593 2064 1317 398 274 147 411
Cuts at: 8484 2100
Size: 2437
Fragments arranged by size:
2437 2064 1593 1317 411 398 274 180
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2621 2651 4039 4069 5340
Size: 30 165 30 1218 30 1388 30 1271
Cuts at: 5340 5370 1178
Size: 30 4629
Fragments arranged by size:
4629 1388 1271 1218 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3176 3544 4019 4340 4775 1186
Size: 1990 368 475 321 435 5232
Fragments arranged by size:
5232 1990 475 435 368 321
BseYI C'CCAG_C
Cuts at: 3172 3238 3736 3992 5727 8024 8520 3172
Size: 66 498 256 1735 2297 496 3473
Fragments arranged by size:
3473 2297 1735 498 496 256 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2261 3251 4061 4291 5234 1163
Size: 573 525 990 810 230 943 4750
Fragments arranged by size:
4750 990 943 810 573 525 230
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3984 6271 7032 8060 1540
Size: 275 186 1983 2287 761 1028 2301
Fragments arranged by size:
2301 2287 1983 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2328 3632 3699 4335 4801 5028 5184 5817 5906
Size: 1304 67 636 466 227 156 633 89
Cuts at: 5906 6882 6967 8038 2328
Size: 976 85 1071 3111
Fragments arranged by size:
3111 1304 1071 976 636 633 466 227
156 89 85 67
BsmI GAATG_Cn'
Cuts at: 6332 6425 6332
Size: 93 8728
BsmBI CGTCTCn'nnnn_
Cuts at: 3616 5330 3616
Size: 1714 7107
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2328 2565 2689 2813 2974 3045 3203
Size: 399 538 237 124 124 161 71 158
Cuts at: 3203 3632 3699 4335 4433 4801 5028 5184 5575
Size: 429 67 636 98 368 227 156 391
Cuts at: 5575 5817 5906 6140 6882 6967 8038 1391
Size: 242 89 234 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 636 538 429 399 391
368 242 237 234 227 161 158 156
124 124 98 89 85 71 67
BspEI T'CCGG_A
Cuts at: 3873 5937 8484 3873
Size: 2064 2547 4210
Fragments arranged by size:
4210 2547 2064
BspHI T'CATG_A
Cuts at: 4725 6710 8440 4725
Size: 1985 1730 5106
Fragments arranged by size:
5106 1985 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2488 3783 4397 4666 5521 6176 844
Size: 1644 1295 614 269 855 655 3489
Fragments arranged by size:
3489 1644 1295 855 655 614 269
BsrBI CCG'CTC
Cuts at: 1394 1823 4492 4918 4939 6708 1394
Size: 429 2669 426 21 1769 3507
Fragments arranged by size:
3507 2669 1769 429 426 21
BsrDI GCAATG_nn'
Cuts at: 65 1248 5627 5995 7316 7490 65
Size: 1183 4379 368 1321 174 1396
Fragments arranged by size:
4379 1396 1321 1183 368 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2047 4621 7465 7652 1700
Size: 110 237 2574 2844 187 2869
Fragments arranged by size:
2869 2844 2574 237 187 110
BsrGI T'GTAC_A
Cuts at: 96 5295 96
Size: 5199 3622
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8821
BssSI C'ACGA_G
Cuts at: 6881 7893 6881
Size: 1012 7809
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2170 3703 4507 5188 794
Size: 1376 1533 804 681 4427
Fragments arranged by size:
4427 1533 1376 804 681
BstBI TT'CG_AA
Cuts at: 2007 7625 2007
Size: 5618 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8821
BstXI CCAn_nnnn'nTGG
Cuts at: 2633 3098 4000 5054 2633
Size: 465 902 1054 6400
Fragments arranged by size:
6400 1054 902 465
BstYI r'GATC_y
Cuts at: 1972 2504 3294 3895 4011 4477 4710 4864 5205
Size: 532 790 601 116 466 233 154 341
Cuts at: 5205 5885 6151 6518 6903 6920 8372 8458 8470
Size: 680 266 367 385 17 1452 86 12
Cuts at: 8470 8816 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 790 680 601 532 466 385
367 346 341 266 233 154 116 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2984 3037 5073 5673 513
Size: 552 957 962 53 2036 600 3661
Fragments arranged by size:
3661 2036 962 957 600 552 53
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4938 487
Size: 683 56 3712 4370
Fragments arranged by size:
4370 3712 683 56
BtsI GCAGTG_nn'
Cuts at: 4303 4423 4431 4670 4778 4917 5182 6337 7131
Size: 120 8 239 108 139 265 1155 794
Cuts at: 7131 7151 4303
Size: 20 5973
Fragments arranged by size:
5973 1155 794 265 239 139 120 108
20 8
ClaI AT'CG_AT
Cuts at: 1554 4243 1554
Size: 2689 6132
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8786
DraI TTT'AAA
Cuts at: 6125 6125
Size: 8821
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7828 809
Size: 507 6512 1802
Fragments arranged by size:
6512 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2049 3003 3049
Size: 54 1425 50 275 18 219 954 46
Cuts at: 3049 3306 3981 4120 4882 5676 5979 6268 7157
Size: 257 675 139 762 794 303 289 889
Cuts at: 7157 8786 8
Size: 1629 43
Fragments arranged by size:
1629 1425 954 889 794 762 675 303
289 275 257 219 139 54 50 46
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3981 6268 1537
Size: 275 2169 2287 4090
Fragments arranged by size:
4090 2287 2169 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2220 2797 3803 4595 4613 5074 5153 5839
Size: 867 577 1006 792 18 461 79 686
Cuts at: 5839 6751 1353
Size: 912 3423
Fragments arranged by size:
3423 1006 912 867 792 686 577 461
79 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2096 7408 7782 7928 387
Size: 1064 138 507 5312 374 146 1280
Fragments arranged by size:
5312 1280 1064 507 374 146 138
EcoICRI GAG'CTC
Cuts at: 2326 3630 5904 2326
Size: 1304 2274 5243
Fragments arranged by size:
5243 2274 1304
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2180 2195 2270 2372 2583 2675 2733
Size: 393 548 15 75 102 211 92 58
Cuts at: 2733 2830 3134 3245 3470 3779 4007 4022 4041
Size: 97 304 111 225 309 228 15 19
Cuts at: 4041 4055 4296 4516 4996 5075 5198 5510 5511
Size: 14 241 220 480 79 123 312 1
Cuts at: 5511 5820 6884 7481 8268 8536 1239
Size: 309 1064 597 787 268 1524
Fragments arranged by size:
1524 1064 787 597 548 480 393 312
309 309 304 268 241 228 225 220
211 123 111 102 97 92 79 75
58 19 15 15 14 1
EcoNI CCTnn'n_nnAGG
Cuts at: 2679 2923 2960 3830 4001 4034 5167 2679
Size: 244 37 870 171 33 1133 6333
Fragments arranged by size:
6333 1133 870 244 171 37 33
EcoO109I rG'GnC_Cy
Cuts at: 3129 3208 4579 5173 5486 5533 5571 5572 3129
Size: 79 1371 594 313 47 38 1 6378
Fragments arranged by size:
6378 1371 594 313 79 47 38 1
EcoRI G'AATT_C
Cuts at: 6130 6508 6130
Size: 378 8443
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5950 5950
Size: 8821
FspI TGC'GCA
Cuts at: 4224 7327 4224
Size: 3103 5718
FspAI rTGC'GCAy
Cuts at: 4224 4224
Size: 8821
HaeII r_GCGC'y
Cuts at: 1678 2048 2488 3817 5560 7968 1678
Size: 370 440 1329 1743 2408 2531
Fragments arranged by size:
2531 2408 1743 1329 440 370
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2443 2475 2621 2653 3101
Size: 32 135 32 871 32 146 32 448
Cuts at: 3101 3133 3482 3506 3514 3538 3650 3682 3887
Size: 32 349 24 8 24 112 32 205
Cuts at: 3887 3919 5147 5179 5204 5236 5888 5920 5932
Size: 32 1228 32 25 32 652 32 12
Cuts at: 5932 5964 7462 7494 7536 7568 1373
Size: 32 1498 32 42 32 2626
Fragments arranged by size:
2626 1498 1228 871 652 448 349 205
146 135 112 42 32 32 32 32
32 32 32 32 32 32 32 32
32 25 24 24 12 8
HincII GTy'rAC
Cuts at: 669 1657 1938 5610 6165 6411 669
Size: 988 281 3672 555 246 3079
Fragments arranged by size:
3672 3079 988 555 281 246
HindIII A'AGCT_T
Cuts at: 748 2444 2834 748
Size: 1696 390 6735
Fragments arranged by size:
6735 1696 390
HpaI GTT'AAC
Cuts at: 6411 6411
Size: 8821
KasI G'GCGC_C
Cuts at: 2044 2044
Size: 8821
KpnI G_GTAC'C
Cuts at: 6146 6146
Size: 8821
MfeI C'AATT_G
Cuts at: 6420 6420
Size: 8821
MluI A'CGCG_T
Cuts at: 5278 8697 5278
Size: 3419 5402
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4876 7935 8119 1305
Size: 3571 3059 184 2007
Fragments arranged by size:
3571 3059 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3005 3308 4122 4884 5678
Size: 54 1425 343 1173 303 814 762 794
Cuts at: 5678 5981 10
Size: 303 2850
Fragments arranged by size:
2850 1425 1173 814 794 762 343 303
303 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4313 4616 4964 5021 5876 6779 7138
Size: 648 3147 303 348 57 855 903 359
Cuts at: 7138 7297 518
Size: 159 2042
Fragments arranged by size:
3147 2042 903 855 648 359 348 303
159 57
MspA1I CmG'CkG
Cuts at: 1606 2350 2663 2986 3265 3576 3909 4740 4906
Size: 744 313 323 279 311 333 831 166
Cuts at: 4906 5095 5579 5587 5731 6046 6237 6914 8062
Size: 189 484 8 144 315 191 677 1148
Cuts at: 8062 8307 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 831 744 677 484 333 323
315 313 311 279 245 191 189 166
144 8
NaeI GCC'GGC
Cuts at: 2049 2049
Size: 8821
NarI GG'CG_CC
Cuts at: 2045 2045
Size: 8821
NcoI C'CATG_G
Cuts at: 513 1065 2022 3037 513
Size: 552 957 1015 6297
Fragments arranged by size:
6297 1015 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8821
NgoMIV G'CCGG_C
Cuts at: 2047 2047
Size: 8821
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8821
NotI GC'GGCC_GC
Cuts at: 6268 6268
Size: 8821
NsiI A_TGCA'T
Cuts at: 2831 2831
Size: 8821
NspI r_CATG'y
Cuts at: 1132 1229 3322 3360 3481 6179 7724 1132
Size: 97 2093 38 121 2698 1545 2229
Fragments arranged by size:
2698 2229 2093 1545 121 97 38
PasI CC'CwG_GG
Cuts at: 1348 2647 2906 3392 4198 1348
Size: 1299 259 486 806 5971
Fragments arranged by size:
5971 1299 806 486 259
PciI A'CATG_T
Cuts at: 7720 7720
Size: 8821
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2620 5489 5619 5685 1108
Size: 1512 2869 130 66 4244
Fragments arranged by size:
4244 2869 1512 130 66
PfoI T'CCnGG_A
Cuts at: 3563 5790 3563
Size: 2227 6594
PmeI GTTT'AAAC
Cuts at: 6125 6125
Size: 8821
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5340 5372 6890 6922 8403 8435 5340
Size: 32 1518 32 1481 32 5726
Fragments arranged by size:
5726 1518 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3129 5173 5533 3129
Size: 2044 360 6417
Fragments arranged by size:
6417 2044 360
PshAI GACnn'nnGTC
Cuts at: 1613 5279 1613
Size: 3666 5155
PsiI TTA'TAA
Cuts at: 6391 6391
Size: 8821
PspOMI G'GGCC_C
Cuts at: 5571 5571
Size: 8821
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5294 5326 5294
Size: 32 8789
PstI C_TGCA'G
Cuts at: 830 2581 2694 3521 3708 4408 4677 4704 5193
Size: 1751 113 827 187 700 269 27 489
Cuts at: 5193 5861 6173 830
Size: 668 312 3478
Fragments arranged by size:
3478 1751 827 700 668 489 312 269
187 113 27
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8821
PvuII CAG'CTG
Cuts at: 2350 2663 4740 5587 5731 6046 2350
Size: 313 2077 847 144 315 5125
Fragments arranged by size:
5125 2077 847 315 313 144
RsrII CG'GwC_CG
Cuts at: 4929 5341 4929
Size: 412 8409
SacI G_AGCT'C
Cuts at: 2328 3632 5906 2328
Size: 1304 2274 5243
Fragments arranged by size:
5243 2274 1304
SacII CC_GC'GG
Cuts at: 2987 2987
Size: 8821
SalI G'TCGA_C
Cuts at: 1936 6163 1936
Size: 4227 4594
SapI GCTCTTCn'nnn_
Cuts at: 4595 4613 5074 4595
Size: 18 461 8342
Fragments arranged by size:
8342 461 18
SbfI CC_TGCA'GG
Cuts at: 5193 6173 5193
Size: 980 7841
ScaI AGT'ACT
Cuts at: 4020 4760 7069 8781 4020
Size: 740 2309 1712 4060
Fragments arranged by size:
4060 2309 1712 740
SfcI C'TryA_G
Cuts at: 826 1045 2111 2577 2690 3517 3704 4404 4516
Size: 219 1066 466 113 827 187 700 112
Cuts at: 4516 4673 4700 5189 5560 5857 6169 7304 7985
Size: 157 27 489 371 297 312 1135 681
Cuts at: 7985 8176 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1066 827 700 681 489 466
371 312 297 219 191 187 157 113
112 27
SfoI GGC'GCC
Cuts at: 2046 2046
Size: 8821
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8821
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8821
SmaI CCC'GGG
Cuts at: 1872 3134 3593 6148 1872
Size: 1262 459 2555 4545
Fragments arranged by size:
4545 2555 1262 459
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2033 2432 2447 3882 4583 5423
Size: 197 926 90 399 15 1435 701 840
Cuts at: 5423 6925 7826 8088 820
Size: 1502 901 262 1553
Fragments arranged by size:
1553 1502 1435 926 901 840 701 399
262 197 90 15
SnaBI TAC'GTA
Cuts at: 493 6118 6612 493
Size: 5625 494 2702
Fragments arranged by size:
5625 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8821
SphI G_CATG'C
Cuts at: 1132 3322 3360 6179 1132
Size: 2190 38 2819 3774
Fragments arranged by size:
3774 2819 2190 38
SspI AAT'ATT
Cuts at: 5 52 6745 5
Size: 47 6693 2081
Fragments arranged by size:
6693 2081 47
StuI AGG'CCT
Cuts at: 1517 3077 4565 1517
Size: 1560 1488 5773
Fragments arranged by size:
5773 1560 1488
StyI C'CwwG_G
Cuts at: 513 1065 2022 2727 3037 3211 4107 4128 4422
Size: 552 957 705 310 174 896 21 294
Cuts at: 4422 5508 5739 6096 6260 513
Size: 1086 231 357 164 3074
Fragments arranged by size:
3074 1086 957 896 705 552 357 310
294 231 174 164 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2204 2413 3535 4006 5358 6848 6865
Size: 1255 624 209 1122 471 1352 1490 17
Cuts at: 6865 7018 7203 7648 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1490 1352 1255 1122 624 471 445
209 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3846 4018 4758
Size: 274 80 33 51 1443 1869 172 740
Cuts at: 4758 5295 5310 7067 8779 96
Size: 537 15 1757 1712 138
Fragments arranged by size:
1869 1757 1712 1443 740 537 274 172
138 80 51 33 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4039 4071 5143 5175 8781 8813 4039
Size: 32 1072 32 3606 32 4047
Fragments arranged by size:
4047 3606 1072 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8821
XbaI T'CTAG_A
Cuts at: 6157 6157
Size: 8821
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3035 3044 3733 5453 5974 3035
Size: 9 689 1720 521 5882
Fragments arranged by size:
5882 1720 689 521 9
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8821
XmaI C'CCGG_G
Cuts at: 1870 3132 3591 6146 1870
Size: 1262 459 2555 4545
Fragments arranged by size:
4545 2555 1262 459
XmnI GAAnn'nnTTC
Cuts at: 2529 4160 6950 2529
Size: 1631 2790 4400
Fragments arranged by size:
4400 2790 1631
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6629 276
Size: 53 83 186 936 5095 2468
Fragments arranged by size:
5095 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BsiWI BstZ17I Bsu36I DraIII
EcoRV FseI NruI PacI PmlI PspXI SanDI SexAI
SfiI SrfI SwaI