(Circular) (Six-base) MAPSORT of: pFN21ASDA1195.seq Check: 3206 from: 1 to: 6856 pFN21ASDA1195 6856 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2148 2269 2283 2148 Size: 121 14 6721 Fragments arranged by size: 6721 121 14 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4666 278 Size: 53 83 186 936 3130 2468 Fragments arranged by size: 3130 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4199 1937 Size: 2262 4594 Acc65I G'GTAC_C Cuts at: 2563 4177 2563 Size: 1614 5242 AclI AA'CG_TT Cuts at: 1563 4983 5356 1563 Size: 3420 373 3063 Fragments arranged by size: 3420 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2419 3147 3454 4034 4919 6303 6571 1632 Size: 787 728 307 580 885 1384 268 1917 Fragments arranged by size: 1917 1384 885 787 728 580 307 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6659 AflIII A'CryG_T Cuts at: 1203 2163 2946 5755 6732 1203 Size: 960 783 2809 977 1327 Fragments arranged by size: 2809 1327 977 960 783 AgeI A'CCGG_T Cuts at: 5687 5687 Size: 6856 AhdI GACnn_n'nnGTC Cuts at: 3520 5585 3520 Size: 2065 4791 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6856 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6856 AlwNI CAG_nnn'CTG Cuts at: 2708 4599 6171 2708 Size: 1891 1572 3393 Fragments arranged by size: 3393 1891 1572 ApaLI G'TGCA_C Cuts at: 2957 4913 6069 2957 Size: 1956 1156 3744 Fragments arranged by size: 3744 1956 1156 ApoI r'AATT_y Cuts at: 1493 2719 3464 3733 4165 4391 4543 6652 1493 Size: 1226 745 269 432 226 152 2109 1697 Fragments arranged by size: 2109 1697 1226 745 432 269 226 152 AseI AT'TA_AT Cuts at: 160 1032 5410 160 Size: 872 4378 1606 Fragments arranged by size: 4378 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6856 AvaI C'yCGr_G Cuts at: 1870 1943 4095 4181 1870 Size: 73 2152 86 4545 Fragments arranged by size: 4545 2152 86 73 BamHI G'GATC_C Cuts at: 4186 4553 4186 Size: 367 6489 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2052 2150 2563 3911 4177 Size: 325 217 627 265 98 413 1348 266 Cuts at: 4177 5632 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1348 627 413 325 266 265 217 98 BanII G_rGCy'C Cuts at: 1391 3106 3399 3642 4175 1391 Size: 1715 293 243 533 4072 Fragments arranged by size: 4072 1715 533 293 243 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5885 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2837 2871 5047 5081 1903 Size: 34 900 34 2176 34 3678 Fragments arranged by size: 3678 2176 900 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3151 4748 5958 3151 Size: 1597 1210 4049 Fragments arranged by size: 4049 1597 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6856 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5467 136 Size: 107 122 71 631 605 3795 1525 Fragments arranged by size: 3795 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3202 3936 6851 3202 Size: 734 2915 3207 Fragments arranged by size: 3207 2915 734 BlpI GC'TnA_GC Cuts at: 3680 4273 3680 Size: 593 6263 Bme1580I G_kGCm'C Cuts at: 1790 2055 2288 2961 4917 6073 1790 Size: 265 233 673 1956 1156 2573 Fragments arranged by size: 2573 1956 1156 673 265 233 BmgBI CAC'GTC Cuts at: 1318 3256 1318 Size: 1938 4918 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2376 4587 5545 Size: 416 53 83 399 24 959 2211 958 Cuts at: 5545 442 Size: 1753 Fragments arranged by size: 2211 1753 959 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2633 1056 Size: 1577 5279 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3059 3206 3657 3776 3940 5516 1239 Size: 1820 147 451 119 164 1576 2579 Fragments arranged by size: 2579 1820 1576 451 164 147 119 Bpu10I CC'TnA_GC Cuts at: 3045 3045 Size: 6856 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3626 4981 5846 6144 3626 Size: 1355 865 298 4338 Fragments arranged by size: 4338 1355 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2369 5519 882 Size: 617 870 3150 2219 Fragments arranged by size: 3150 2219 870 617 BsaAI yAC'GTr Cuts at: 493 1204 3391 4153 4647 493 Size: 711 2187 762 494 2702 Fragments arranged by size: 2702 2187 762 711 494 BsaBI GATnn'nnATC Cuts at: 6850 6850 Size: 6856 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4663 5045 5680 275 Size: 53 83 186 936 3130 382 635 1451 Fragments arranged by size: 3130 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5289 5687 5961 6108 6519 5289 Size: 398 274 147 411 5626 Fragments arranged by size: 5626 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2523 2553 1178 Size: 30 165 30 1120 30 5481 Fragments arranged by size: 5481 1120 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2180 2402 2501 2547 1186 Size: 994 222 99 46 5495 Fragments arranged by size: 5495 994 222 99 46 BseYI C'CCAG_C Cuts at: 3776 3804 3975 6059 6555 3776 Size: 28 171 2084 496 4077 Fragments arranged by size: 4077 2084 496 171 28 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3476 1163 Size: 573 1740 4543 Fragments arranged by size: 4543 1740 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4306 5067 6095 1540 Size: 275 186 2305 761 1028 2301 Fragments arranged by size: 2305 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2184 2700 2961 3106 3399 3642 4011 4044 4917 Size: 516 261 145 293 243 369 33 873 Cuts at: 4917 5002 6073 2184 Size: 85 1071 2967 Fragments arranged by size: 2967 1071 873 516 369 293 261 243 145 85 33 BsmI GAATG_Cn' Cuts at: 4367 4460 4367 Size: 93 6763 BsmBI CGTCTCn'nnnn_ Cuts at: 4150 4150 Size: 6856 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2055 2184 2288 2700 2961 3106 3399 Size: 399 265 129 104 412 261 145 293 Cuts at: 3399 3642 4011 4044 4175 4917 5002 6073 1391 Size: 243 369 33 131 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 412 399 369 293 265 261 243 145 131 129 104 85 33 BspEI T'CCGG_A Cuts at: 6519 6519 Size: 6856 BspHI T'CATG_A Cuts at: 2356 2367 4745 6475 2356 Size: 11 2378 1730 2737 Fragments arranged by size: 2737 2378 1730 11 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2148 2197 2269 2283 3198 4211 844 Size: 1304 49 72 14 915 1013 3489 Fragments arranged by size: 3489 1304 1013 915 72 49 14 BsrBI CCG'CTC Cuts at: 1394 1823 4743 1394 Size: 429 2920 3507 Fragments arranged by size: 3507 2920 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3771 3887 5351 5525 65 Size: 1183 2523 116 1464 174 1396 Fragments arranged by size: 2523 1464 1396 1183 174 116 BsrFI r'CCGG_y Cuts at: 1700 1810 5500 5687 1700 Size: 110 3690 187 2869 Fragments arranged by size: 3690 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3002 3575 96 Size: 2906 573 3377 Fragments arranged by size: 3377 2906 573 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6856 BssSI C'ACGA_G Cuts at: 4131 4916 5928 4131 Size: 785 1012 5059 Fragments arranged by size: 5059 1012 785 BstAPI GCAn_nnn'nTGC Cuts at: 794 2708 794 Size: 1914 4942 BstBI TT'CG_AA Cuts at: 2007 5660 2007 Size: 3653 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6856 BstYI r'GATC_y Cuts at: 1972 2039 2438 3202 3936 4186 4553 4938 4955 Size: 67 399 764 734 250 367 385 17 Cuts at: 4955 6407 6493 6505 6851 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 764 734 399 385 367 346 250 86 67 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2310 2865 3904 513 Size: 552 957 288 555 1039 3465 Fragments arranged by size: 3465 1039 957 555 552 288 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6117 Fragments arranged by size: 6117 683 56 BtsI GCAGTG_nn' Cuts at: 2086 2544 3159 4372 5166 5186 2086 Size: 458 615 1213 794 20 3756 Fragments arranged by size: 3756 1213 794 615 458 20 ClaI AT'CG_AT Cuts at: 1554 3550 1554 Size: 1996 4860 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2045 2080 2863 2898 529 Size: 35 1481 35 783 35 4487 Fragments arranged by size: 4487 1481 783 35 35 35 DraI TTT'AAA Cuts at: 4160 4160 Size: 6856 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5863 809 Size: 507 4547 1802 Fragments arranged by size: 4547 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2265 2327 2668 Size: 54 1425 50 275 18 435 62 341 Cuts at: 2668 2782 4303 5192 6821 8 Size: 114 1521 889 1629 43 Fragments arranged by size: 1629 1521 1425 889 435 341 275 114 62 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4303 1537 Size: 275 2491 4090 Fragments arranged by size: 4090 2491 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2438 3962 4786 1353 Size: 1085 1524 824 3423 Fragments arranged by size: 3423 1524 1085 824 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2983 5443 5817 5963 387 Size: 1064 138 1394 2460 374 146 1280 Fragments arranged by size: 2460 1394 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3104 3397 3640 3104 Size: 293 243 6320 Fragments arranged by size: 6320 293 243 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2419 3059 3147 3206 3454 3657 3776 Size: 393 787 640 88 59 248 203 119 Cuts at: 3776 3940 4034 4919 5516 6303 6571 1239 Size: 164 94 885 597 787 268 1524 Fragments arranged by size: 1524 885 787 787 640 597 393 268 248 203 164 119 94 88 59 EcoO109I rG'GnC_Cy Cuts at: 2428 3187 2428 Size: 759 6097 EcoRI G'AATT_C Cuts at: 3733 4165 4543 3733 Size: 432 378 6046 Fragments arranged by size: 6046 432 378 EcoRV GAT'ATC Cuts at: 2554 2554 Size: 6856 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2449 2449 Size: 6856 FspI TGC'GCA Cuts at: 5362 5362 Size: 6856 HaeII r_GCGC'y Cuts at: 1678 2811 6003 1678 Size: 1133 3192 2531 Fragments arranged by size: 3192 2531 1133 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2538 2570 3506 3538 3543 Size: 32 135 32 966 32 936 32 5 Cuts at: 3543 3575 3855 3887 5497 5529 5571 5603 1373 Size: 32 280 32 1610 32 42 32 2626 Fragments arranged by size: 2626 1610 966 936 280 135 42 32 32 32 32 32 32 32 32 5 HincII GTy'rAC Cuts at: 669 1657 1938 4200 4446 669 Size: 988 281 2262 246 3079 Fragments arranged by size: 3079 2262 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6856 HpaI GTT'AAC Cuts at: 4446 4446 Size: 6856 KpnI G_GTAC'C Cuts at: 2567 4181 2567 Size: 1614 5242 MfeI C'AATT_G Cuts at: 4455 4455 Size: 6856 MluI A'CGCG_T Cuts at: 6732 6732 Size: 6856 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5970 6154 1305 Size: 4665 184 2007 Fragments arranged by size: 4665 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2267 2329 2670 2784 10 Size: 54 1425 343 435 62 341 114 4082 Fragments arranged by size: 4082 1425 435 343 341 114 62 54 MslI CAynn'nnrTG Cuts at: 518 1166 2099 3998 4814 5173 5332 518 Size: 648 933 1899 816 359 159 2042 Fragments arranged by size: 2042 1899 933 816 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2325 2924 3368 4272 4949 6097 6342 1606 Size: 719 599 444 904 677 1148 245 2120 Fragments arranged by size: 2120 1148 904 719 677 599 444 245 NcoI C'CATG_G Cuts at: 513 1065 2022 2310 2865 3904 513 Size: 552 957 288 555 1039 3465 Fragments arranged by size: 3465 1039 957 555 552 288 NdeI CA'TA_TG Cuts at: 387 387 Size: 6856 NheI G'CTAG_C Cuts at: 1052 2629 1052 Size: 1577 5279 NotI GC'GGCC_GC Cuts at: 4303 4303 Size: 6856 NspI r_CATG'y Cuts at: 1132 1229 2167 2950 4075 4214 5759 1132 Size: 97 938 783 1125 139 1545 2229 Fragments arranged by size: 2229 1545 1125 938 783 139 97 PasI CC'CwG_GG Cuts at: 1348 3377 3605 1348 Size: 2029 228 4599 Fragments arranged by size: 4599 2029 228 PciI A'CATG_T Cuts at: 2163 2946 5755 2163 Size: 783 2809 3264 Fragments arranged by size: 3264 2809 783 PflMI CCAn_nnn'nTGG Cuts at: 1108 2263 2316 2791 3709 1108 Size: 1155 53 475 918 4255 Fragments arranged by size: 4255 1155 918 475 53 PfoI T'CCnGG_A Cuts at: 3917 3917 Size: 6856 PmeI GTTT'AAAC Cuts at: 4160 4160 Size: 6856 PmlI CAC'GTG Cuts at: 3391 3391 Size: 6856 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4925 4957 6438 6470 4925 Size: 32 1481 32 5311 Fragments arranged by size: 5311 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2428 3187 2428 Size: 759 6097 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6856 PsiI TTA'TAA Cuts at: 4426 4426 Size: 6856 PstI C_TGCA'G Cuts at: 830 2280 2418 4208 830 Size: 1450 138 1790 3478 Fragments arranged by size: 3478 1790 1450 138 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6856 PvuII CAG'CTG Cuts at: 2924 2924 Size: 6856 SacI G_AGCT'C Cuts at: 3106 3399 3642 3106 Size: 293 243 6320 Fragments arranged by size: 6320 293 243 SalI G'TCGA_C Cuts at: 1936 4198 1936 Size: 2262 4594 SbfI CC_TGCA'GG Cuts at: 2280 2418 4208 2280 Size: 138 1790 4928 Fragments arranged by size: 4928 1790 138 ScaI AGT'ACT Cuts at: 5104 6816 5104 Size: 1712 5144 SexAI A'CCwGG_T Cuts at: 2655 2655 Size: 6856 SfcI C'TryA_G Cuts at: 826 1045 2276 2414 3628 4204 5339 6020 6211 Size: 219 1231 138 1214 576 1135 681 191 Cuts at: 6211 826 Size: 1471 Fragments arranged by size: 1471 1231 1214 1135 681 576 219 191 138 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6856 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6856 SmaI CCC'GGG Cuts at: 1872 4183 1872 Size: 2311 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3641 4960 5861 6123 820 Size: 197 926 1698 1319 901 262 1553 Fragments arranged by size: 1698 1553 1319 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4153 4647 493 Size: 3660 494 2702 Fragments arranged by size: 3660 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6856 SphI G_CATG'C Cuts at: 1132 4214 1132 Size: 3082 3774 SspI AAT'ATT Cuts at: 5 52 2683 4780 5 Size: 47 2631 2097 2081 Fragments arranged by size: 2631 2097 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6856 StyI C'CwwG_G Cuts at: 513 1065 2022 2310 2865 2888 3009 3904 4295 Size: 552 957 288 555 23 121 895 391 Cuts at: 4295 513 Size: 3074 Fragments arranged by size: 3074 957 895 555 552 391 288 121 23 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2531 3022 4883 4900 5053 5238 5683 Size: 1255 951 491 1861 17 153 185 445 Cuts at: 5683 325 Size: 1498 Fragments arranged by size: 1861 1498 1255 951 491 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3002 3575 3768 Size: 274 80 33 51 1443 1025 573 193 Cuts at: 3768 5102 6814 96 Size: 1334 1712 138 Fragments arranged by size: 1712 1443 1334 1025 573 274 193 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2523 2555 3375 3407 3826 3858 4001 4033 6816 Size: 32 820 32 419 32 143 32 2783 Cuts at: 6816 6848 2523 Size: 32 2531 Fragments arranged by size: 2783 2531 820 419 143 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2657 3791 1316 Size: 1341 1134 4381 Fragments arranged by size: 4381 1341 1134 XbaI T'CTAG_A Cuts at: 4192 4192 Size: 6856 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2144 2647 3751 2144 Size: 503 1104 5249 Fragments arranged by size: 5249 1104 503 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6856 XmaI C'CCGG_G Cuts at: 1870 4181 1870 Size: 2311 4545 XmnI GAAnn'nnTTC Cuts at: 4985 4985 Size: 6856 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4664 276 Size: 53 83 186 936 3130 2468 Fragments arranged by size: 3130 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI