Restriction map for
pFN21ASDA1187
(Circular) (Six-base) MAPSORT of: pFN21ASDA1187.seq Check: 1664 from: 1 to: 7138
pFN21ASDA1187 7138 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4948 278
Size: 53 83 186 936 3412 2468
Fragments arranged by size:
3412 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4481 1937
Size: 2544 4594
Acc65I G'GTAC_C
Cuts at: 4459 4459
Size: 7138
AclI AA'CG_TT
Cuts at: 1563 5265 5638 1563
Size: 3702 373 3063
Fragments arranged by size:
3702 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2310 5201 6585 6853 1632
Size: 678 2891 1384 268 1917
Fragments arranged by size:
2891 1917 1384 678 268
AfeI AGC'GCT
Cuts at: 2651 2711 2651
Size: 60 7078
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6941
AflIII A'CryG_T
Cuts at: 1203 6037 7014 1203
Size: 4834 977 1327
Fragments arranged by size:
4834 1327 977
AgeI A'CCGG_T
Cuts at: 5969 5969
Size: 7138
AhdI GACnn_n'nnGTC
Cuts at: 5867 5867
Size: 7138
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7138
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2616 3357 4262 1184
Size: 1432 741 905 4060
Fragments arranged by size:
4060 1432 905 741
AlwNI CAG_nnn'CTG
Cuts at: 3238 3623 3650 3985 4142 4881 6453 3238
Size: 385 27 335 157 739 1572 3923
Fragments arranged by size:
3923 1572 739 385 335 157 27
ApaI G_GGCC'C
Cuts at: 2330 2731 3480 2330
Size: 401 749 5988
Fragments arranged by size:
5988 749 401
ApaLI G'TGCA_C
Cuts at: 3047 5195 6351 3047
Size: 2148 1156 3834
Fragments arranged by size:
3834 2148 1156
ApoI r'AATT_y
Cuts at: 1493 4447 4673 4825 6934 1493
Size: 2954 226 152 2109 1697
Fragments arranged by size:
2954 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5692 160
Size: 872 4660 1606
Fragments arranged by size:
4660 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7138
AvaI C'yCGr_G
Cuts at: 1870 1943 2633 3207 3402 3479 3741 3837 4463
Size: 73 690 574 195 77 262 96 626
Cuts at: 4463 1870
Size: 4545
Fragments arranged by size:
4545 690 626 574 262 195 96 77
73
AvrII C'CTAG_G
Cuts at: 2252 2252
Size: 7138
BamHI G'GATC_C
Cuts at: 4468 4835 4468
Size: 367 6771
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2559 3407 3547 3559 3686
Size: 325 217 627 772 848 140 12 127
Cuts at: 3686 3732 4459 5914 618
Size: 46 727 1455 1842
Fragments arranged by size:
1842 1455 848 772 727 627 325 217
140 127 46 12
BanII G_rGCy'C
Cuts at: 1391 2234 2330 2731 3288 3464 3480 4206 4457
Size: 843 96 401 557 176 16 726 251
Cuts at: 4457 1391
Size: 4072
Fragments arranged by size:
4072 843 726 557 401 251 176 96
16
BbeI G_GCGC'C
Cuts at: 2563 3563 3736 2563
Size: 1000 173 5965
Fragments arranged by size:
5965 1000 173
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2690 928
Size: 971 791 5376
Fragments arranged by size:
5376 971 791
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5329 5363 1903
Size: 34 3392 34 3678
Fragments arranged by size:
3678 3392 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5030 6240 5030
Size: 1210 5928
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7138
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2492 2939 3545
Size: 107 122 71 631 605 820 447 606
Cuts at: 3545 5749 136
Size: 2204 1525
Fragments arranged by size:
2204 1525 820 631 606 605 447 122
107 71
BglII A'GATC_T
Cuts at: 7133 7133
Size: 7138
BlpI GC'TnA_GC
Cuts at: 2777 3287 4555 2777
Size: 510 1268 5360
Fragments arranged by size:
5360 1268 510
Bme1580I G_kGCm'C
Cuts at: 1790 2330 2731 2996 3051 3202 3259 3480 5199
Size: 540 401 265 55 151 57 221 1719
Cuts at: 5199 6355 1790
Size: 1156 2573
Fragments arranged by size:
2573 1719 1156 540 401 265 221 151
57 55
BmgBI CAC'GTC
Cuts at: 1318 2688 1318
Size: 1370 5768
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4869 5827 442
Size: 416 53 83 399 24 3452 958 1753
Fragments arranged by size:
3452 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7138
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2251 2251
Size: 7138
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2091 2525 2531 2588 2630 2730 4007 4067
Size: 852 434 6 57 42 100 1277 60
Cuts at: 4067 5798 1239
Size: 1731 2579
Fragments arranged by size:
2579 1731 1277 852 434 100 60 57
42 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3206 4373 5263 6128 6426 3206
Size: 1167 890 865 298 3918
Fragments arranged by size:
3918 1167 890 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3602 5801 882
Size: 617 2103 2199 2219
Fragments arranged by size:
2219 2199 2103 617
BsaAI yAC'GTr
Cuts at: 493 1204 2916 3254 4435 4929 493
Size: 711 1712 338 1181 494 2702
Fragments arranged by size:
2702 1712 1181 711 494 338
BsaBI GATnn'nnATC
Cuts at: 7132 7132
Size: 7138
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2560 2816 3023 3560
Size: 53 83 186 936 1027 256 207 537
Cuts at: 3560 3733 4945 5327 5962 275
Size: 173 1212 382 635 1451
Fragments arranged by size:
1451 1212 1027 936 635 537 382 256
207 186 173 83 53
BsaWI w'CCGG_w
Cuts at: 3115 5571 5969 6243 6390 6801 3115
Size: 2456 398 274 147 411 3452
Fragments arranged by size:
3452 2456 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3367 3397 4148 4178 4208
Size: 30 165 30 1964 30 751 30 30
Cuts at: 4208 4238 1178
Size: 30 4078
Fragments arranged by size:
4078 1964 751 165 30 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2337 2528 2585 3461 3776 3824 3887 3926
Size: 1151 191 57 876 315 48 63 39
Cuts at: 3926 3956 4064 4214 1186
Size: 30 108 150 4110
Fragments arranged by size:
4110 1151 876 315 191 150 108 63
57 48 39 30
BseYI C'CCAG_C
Cuts at: 2047 2065 3356 3464 3535 3574 3643 4282 6341
Size: 18 1291 108 71 39 69 639 2059
Cuts at: 6341 6837 2047
Size: 496 2348
Fragments arranged by size:
2348 2059 1291 639 496 108 71 69
39 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2622 2717 3049 3167 4211 4424 1163
Size: 573 886 95 332 118 1044 213 3877
Fragments arranged by size:
3877 1044 886 573 332 213 118 95
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2875 2962 3131 3959 4588 5349
Size: 275 186 874 87 169 828 629 761
Cuts at: 5349 6377 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 874 828 761 629 275 186
169 87
BsiHKAI G_wGCw'C
Cuts at: 3051 3288 5199 5284 6355 3051
Size: 237 1911 85 1071 3834
Fragments arranged by size:
3834 1911 1071 237 85
BsmI GAATG_Cn'
Cuts at: 2197 3807 4649 4742 2197
Size: 1610 842 93 4593
Fragments arranged by size:
4593 1610 842 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2234 2330 2731 2996 3051 3202 3259
Size: 399 444 96 401 265 55 151 57
Cuts at: 3259 3288 3464 3480 4206 4457 5199 5284 6355
Size: 29 176 16 726 251 742 85 1071
Cuts at: 6355 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 726 444 401 399 265
251 176 151 96 85 57 55 29
16
BspEI T'CCGG_A
Cuts at: 6801 6801
Size: 7138
BspHI T'CATG_A
Cuts at: 4056 5027 6757 4056
Size: 971 1730 4437
Fragments arranged by size:
4437 1730 971
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2233 4405 4493 844
Size: 1389 2172 88 3489
Fragments arranged by size:
3489 2172 1389 88
BsrBI CCG'CTC
Cuts at: 1394 1823 2031 2455 2467 3113 3175 3772 3847
Size: 429 208 424 12 646 62 597 75
Cuts at: 3847 3922 3976 3991 4024 5025 1394
Size: 75 54 15 33 1001 3507
Fragments arranged by size:
3507 1001 646 597 429 424 208 75
75 62 54 33 15 12
BsrDI GCAATG_nn'
Cuts at: 65 1248 5633 5807 65
Size: 1183 4385 174 1396
Fragments arranged by size:
4385 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2556 2562 2986 3065 3126 3142 3544
Size: 110 746 6 424 79 61 16 402
Cuts at: 3544 3556 3683 5782 5969 1700
Size: 12 127 2099 187 2869
Fragments arranged by size:
2869 2099 746 424 402 187 127 110
79 61 16 12 6
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7138
BssHII G'CGCG_C
Cuts at: 1925 2942 1925
Size: 1017 6121
BssSI C'ACGA_G
Cuts at: 3885 5198 6210 3885
Size: 1313 1012 4813
Fragments arranged by size:
4813 1313 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7138
BstBI TT'CG_AA
Cuts at: 2007 5942 2007
Size: 3935 3203
BstEII G'GTnAC_C
Cuts at: 1184 2114 3661 1184
Size: 930 1547 4661
Fragments arranged by size:
4661 1547 930
BstXI CCAn_nnnn'nTGG
Cuts at: 2479 4298 2479
Size: 1819 5319
BstYI r'GATC_y
Cuts at: 1972 4468 4835 5220 5237 6689 6775 6787 7133
Size: 2496 367 385 17 1452 86 12 346
Cuts at: 7133 1972
Size: 1977
Fragments arranged by size:
2496 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2236 2236
Size: 7138
BtgI C'CryG_G
Cuts at: 513 1065 2022 2322 2330 3603 513
Size: 552 957 300 8 1273 4048
Fragments arranged by size:
4048 1273 957 552 300 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6399
Fragments arranged by size:
6399 683 56
BtsI GCAGTG_nn'
Cuts at: 2385 2502 2858 3411 4654 5448 5468 2385
Size: 117 356 553 1243 794 20 4055
Fragments arranged by size:
4055 1243 794 553 356 117 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7138
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7103
DraI TTT'AAA
Cuts at: 4442 4442
Size: 7138
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3626 4072 6145 809
Size: 507 2310 446 2073 1802
Fragments arranged by size:
2310 2073 1802 507 446
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2484 2872 3128
Size: 54 1425 50 275 18 654 388 256
Cuts at: 3128 3681 4585 5474 7103 8
Size: 553 904 889 1629 43
Fragments arranged by size:
1629 1425 904 889 654 553 388 275
256 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2872 3128 4585 1537
Size: 275 1060 256 1457 4090
Fragments arranged by size:
4090 1457 1060 275 256
EarI CTCTTCn'nnn_
Cuts at: 1353 2268 2457 3188 3960 4055 5068 1353
Size: 915 189 731 772 95 1013 3423
Fragments arranged by size:
3423 1013 915 772 731 189 95
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2998 3256 3857 3869 3986 5725
Size: 1064 138 1409 258 601 12 117 1739
Cuts at: 5725 6099 6245 387
Size: 374 146 1280
Fragments arranged by size:
1739 1409 1280 1064 601 374 258 146
138 117 12
EcoICRI GAG'CTC
Cuts at: 3286 3286
Size: 7138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2091 2310 2525 2531 2588 2630 2730
Size: 393 459 219 215 6 57 42 100
Cuts at: 2730 4007 4067 5201 5798 6585 6853 1239
Size: 1277 60 1134 597 787 268 1524
Fragments arranged by size:
1524 1277 1134 787 597 459 393 268
219 215 100 60 57 42 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2904 3334 2904
Size: 430 6708
EcoO109I rG'GnC_Cy
Cuts at: 2082 2306 2727 2728 2909 3476 2082
Size: 224 421 1 181 567 5744
Fragments arranged by size:
5744 567 421 224 181 1
EcoRI G'AATT_C
Cuts at: 4447 4825 4447
Size: 378 6760
FspI TGC'GCA
Cuts at: 5644 5644
Size: 7138
HaeII r_GCGC'y
Cuts at: 1678 2531 2557 2563 2653 2713 3085 3232 3471
Size: 853 26 6 90 60 372 147 239
Cuts at: 3471 3563 3736 6285 1678
Size: 92 173 2549 2531
Fragments arranged by size:
2549 2531 853 372 239 173 147 92
90 60 26 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2806 2838 3509 3541 4148
Size: 32 135 32 1234 32 671 32 607
Cuts at: 4148 4180 5779 5811 5853 5885 1373
Size: 32 1599 32 42 32 2626
Fragments arranged by size:
2626 1599 1234 671 607 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4482 4728 669
Size: 988 281 2544 246 3079
Fragments arranged by size:
3079 2544 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7138
HpaI GTT'AAC
Cuts at: 4728 4728
Size: 7138
KasI G'GCGC_C
Cuts at: 2559 3559 3732 2559
Size: 1000 173 5965
Fragments arranged by size:
5965 1000 173
KpnI G_GTAC'C
Cuts at: 4463 4463
Size: 7138
MfeI C'AATT_G
Cuts at: 4737 4737
Size: 7138
MluI A'CGCG_T
Cuts at: 7014 7014
Size: 7138
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2396 2415 2692 6252 6436 1305
Size: 1091 19 277 3560 184 2007
Fragments arranged by size:
3560 2007 1091 277 184 19
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5316
Fragments arranged by size:
5316 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5096 5455 5614 518
Size: 648 3930 359 159 2042
Fragments arranged by size:
3930 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2069 2623 2776 3054 3605 3751 3817 4554
Size: 463 554 153 278 551 146 66 737
Cuts at: 4554 5231 6379 6624 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 737 677 554 551 463 278
245 153 146 66
NaeI GCC'GGC
Cuts at: 2558 2564 3128 3558 3685 2558
Size: 6 564 430 127 6011
Fragments arranged by size:
6011 564 430 127 6
NarI GG'CG_CC
Cuts at: 2560 3560 3733 2560
Size: 1000 173 5965
Fragments arranged by size:
5965 1000 173
NcoI C'CATG_G
Cuts at: 513 1065 2022 2322 513
Size: 552 957 300 5329
Fragments arranged by size:
5329 957 552 300
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7138
NgoMIV G'CCGG_C
Cuts at: 2556 2562 3126 3556 3683 2556
Size: 6 564 430 127 6011
Fragments arranged by size:
6011 564 430 127 6
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7138
NotI GC'GGCC_GC
Cuts at: 4585 4585
Size: 7138
NspI r_CATG'y
Cuts at: 1132 1229 4496 6041 1132
Size: 97 3267 1545 2229
Fragments arranged by size:
3267 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2900 3362 4169 1348
Size: 1552 462 807 4317
Fragments arranged by size:
4317 1552 807 462
PciI A'CATG_T
Cuts at: 6037 6037
Size: 7138
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3330 1108
Size: 2222 4916
PfoI T'CCnGG_A
Cuts at: 2547 2746 2547
Size: 199 6939
PmeI GTTT'AAAC
Cuts at: 4442 4442
Size: 7138
PmlI CAC'GTG
Cuts at: 2916 3254 2916
Size: 338 6800
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5207 5239 6720 6752 5207
Size: 32 1481 32 5593
Fragments arranged by size:
5593 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2082 2306 2082
Size: 224 6914
PshAI GACnn'nnGTC
Cuts at: 1613 4332 1613
Size: 2719 4419
PsiI TTA'TAA
Cuts at: 4708 4708
Size: 7138
PspOMI G'GGCC_C
Cuts at: 2326 2727 3476 2326
Size: 401 749 5988
Fragments arranged by size:
5988 749 401
PstI C_TGCA'G
Cuts at: 830 2775 2980 3783 3930 4179 4275 4490 830
Size: 1945 205 803 147 249 96 215 3478
Fragments arranged by size:
3478 1945 803 249 215 205 147 96
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7138
PvuII CAG'CTG
Cuts at: 2069 2776 3817 2069
Size: 707 1041 5390
Fragments arranged by size:
5390 1041 707
SacI G_AGCT'C
Cuts at: 3288 3288
Size: 7138
SacII CC_GC'GG
Cuts at: 3606 3606
Size: 7138
SalI G'TCGA_C
Cuts at: 1936 4480 1936
Size: 2544 4594
SapI GCTCTTCn'nnn_
Cuts at: 2457 2457
Size: 7138
SbfI CC_TGCA'GG
Cuts at: 4490 4490
Size: 7138
ScaI AGT'ACT
Cuts at: 5386 7098 5386
Size: 1712 5426
SfcI C'TryA_G
Cuts at: 826 1045 2771 2976 3615 3779 3926 4175 4271
Size: 219 1726 205 639 164 147 249 96
Cuts at: 4271 4486 5621 6302 6493 826
Size: 215 1135 681 191 1471
Fragments arranged by size:
1726 1471 1135 681 639 249 219 215
205 191 164 147 96
SfoI GGC'GCC
Cuts at: 2561 3561 3734 2561
Size: 1000 173 5965
Fragments arranged by size:
5965 1000 173
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7138
SgrAI Cr'CCGG_yG
Cuts at: 1700 2556 3065 3142 3556 1700
Size: 856 509 77 414 5282
Fragments arranged by size:
5282 856 509 414 77
SmaI CCC'GGG
Cuts at: 1872 3209 3743 3839 4465 1872
Size: 1337 534 96 626 4545
Fragments arranged by size:
4545 1337 626 534 96
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2633 3221 4388 5242 6143 6405
Size: 197 926 690 588 1167 854 901 262
Cuts at: 6405 820
Size: 1553
Fragments arranged by size:
1553 1167 926 901 854 690 588 262
197
SnaBI TAC'GTA
Cuts at: 493 4435 4929 493
Size: 3942 494 2702
Fragments arranged by size:
3942 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7138
SphI G_CATG'C
Cuts at: 1132 4496 1132
Size: 3364 3774
SrfI GCCC'GGGC
Cuts at: 3839 3839
Size: 7138
SspI AAT'ATT
Cuts at: 5 52 5062 5
Size: 47 5010 2081
Fragments arranged by size:
5010 2081 47
StuI AGG'CCT
Cuts at: 1517 3137 3340 1517
Size: 1620 203 5315
Fragments arranged by size:
5315 1620 203
StyI C'CwwG_G
Cuts at: 513 1065 2022 2252 2322 2523 3300 3423 4577
Size: 552 957 230 70 201 777 123 1154
Cuts at: 4577 513
Size: 3074
Fragments arranged by size:
3074 1154 957 777 552 230 201 123
70
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3711 3945 4152 5165 5182 5335 5520
Size: 1255 2131 234 207 1013 17 153 185
Cuts at: 5520 5965 325
Size: 445 1498
Fragments arranged by size:
2131 1498 1255 1013 445 234 207 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5384 7096 96
Size: 274 80 33 51 1443 3407 1712 138
Fragments arranged by size:
3407 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3367 3399 4208 4240 7098 7130 3367
Size: 32 809 32 2858 32 3375
Fragments arranged by size:
3375 2858 809 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7138
XbaI T'CTAG_A
Cuts at: 4474 4474
Size: 7138
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3715 3715
Size: 7138
XhoI C'TCGA_G
Cuts at: 1943 2633 1943
Size: 690 6448
XmaI C'CCGG_G
Cuts at: 1870 3207 3741 3837 4463 1870
Size: 1337 534 96 626 4545
Fragments arranged by size:
4545 1337 626 534 96
XmnI GAAnn'nnTTC
Cuts at: 4301 5267 4301
Size: 966 6172
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4946 276
Size: 53 83 186 936 3412 2468
Fragments arranged by size:
3412 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI BaeI BbvCI BfrBI Bpu10I BsiWI
BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI NruI
NsiI PacI PspXI PsrI RsrII SanDI SexAI SfiI
SwaI