Restriction map for
pFN21ASDA1186
(Circular) (Six-base) MAPSORT of: pFN21ASDA1186.seq Check: 988 from: 1 to: 5938
pFN21ASDA1186 5938 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3092 3092
Size: 5938
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3748 278
Size: 53 83 186 936 2212 2468
Fragments arranged by size:
2468 2212 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3281 1937
Size: 1344 4594
Acc65I G'GTAC_C
Cuts at: 3259 3259
Size: 5938
AclI AA'CG_TT
Cuts at: 1563 4065 4438 1563
Size: 2502 373 3063
Fragments arranged by size:
3063 2502 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2600 3002 3087 4001 5385 5653 1632
Size: 968 402 85 914 1384 268 1917
Fragments arranged by size:
1917 1384 968 914 402 268 85
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5741
AflIII A'CryG_T
Cuts at: 1203 4837 5814 1203
Size: 3634 977 1327
Fragments arranged by size:
3634 1327 977
AgeI A'CCGG_T
Cuts at: 4769 4769
Size: 5938
AhdI GACnn_n'nnGTC
Cuts at: 4667 4667
Size: 5938
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5938
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2442 1184
Size: 1258 4680
AlwNI CAG_nnn'CTG
Cuts at: 2272 2380 3681 5253 2272
Size: 108 1301 1572 2957
Fragments arranged by size:
2957 1572 1301 108
ApaLI G'TGCA_C
Cuts at: 3995 5151 3995
Size: 1156 4782
ApoI r'AATT_y
Cuts at: 1493 3247 3473 3625 5734 1493
Size: 1754 226 152 2109 1697
Fragments arranged by size:
2109 1754 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4492 160
Size: 872 3460 1606
Fragments arranged by size:
3460 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5938
AvaI C'yCGr_G
Cuts at: 1870 1943 3263 1870
Size: 73 1320 4545
Fragments arranged by size:
4545 1320 73
BamHI G'GATC_C
Cuts at: 3268 3635 3268
Size: 367 5571
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2731 2750 3221 3259 4714
Size: 325 217 627 944 19 471 38 1455
Cuts at: 4714 618
Size: 1842
Fragments arranged by size:
1842 1455 944 627 471 325 217 38
19
BanII G_rGCy'C
Cuts at: 1391 2051 3257 1391
Size: 660 1206 4072
Fragments arranged by size:
4072 1206 660
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2990 928
Size: 971 1091 3876
Fragments arranged by size:
3876 1091 971
BbvCI CC'TCA_GC
Cuts at: 2384 2384
Size: 5938
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4129 4163 1903
Size: 34 2192 34 3678
Fragments arranged by size:
3678 2192 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3830 5040 3830
Size: 1210 4728
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5938
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4549 136
Size: 107 122 71 631 605 2877 1525
Fragments arranged by size:
2877 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5933 5933
Size: 5938
BlpI GC'TnA_GC
Cuts at: 3355 3355
Size: 5938
Bme1580I G_kGCm'C
Cuts at: 1790 2431 2753 3999 5155 1790
Size: 641 322 1246 1156 2573
Fragments arranged by size:
2573 1246 1156 641 322
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5938
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2306 3669 4627
Size: 416 53 83 399 24 889 1363 958
Cuts at: 4627 442
Size: 1753
Fragments arranged by size:
1753 1363 958 889 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5938
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2561 2561
Size: 5938
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2235 4598 1239
Size: 996 2363 2579
Fragments arranged by size:
2579 2363 996
Bpu10I CC'TnA_GC
Cuts at: 2384 2384
Size: 5938
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2486 4063 4928 5226 2486
Size: 1577 865 298 3198
Fragments arranged by size:
3198 1577 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2456 2829 4601 882
Size: 617 957 373 1772 2219
Fragments arranged by size:
2219 1772 957 617 373
BsaAI yAC'GTr
Cuts at: 493 1204 3235 3729 493
Size: 711 2031 494 2702
Fragments arranged by size:
2702 2031 711 494
BsaBI GATnn'nnATC
Cuts at: 5932 5932
Size: 5938
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3745 4127 4762 275
Size: 53 83 186 936 2212 382 635 1451
Fragments arranged by size:
2212 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2190 4371 4769 5043 5190 5601 2190
Size: 2181 398 274 147 411 2527
Fragments arranged by size:
2527 2181 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2946 2976 1178
Size: 30 165 30 1543 30 4140
Fragments arranged by size:
4140 1543 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2875 1186
Size: 1689 4249
BseYI C'CCAG_C
Cuts at: 3211 5141 5637 3211
Size: 1930 496 3512
Fragments arranged by size:
3512 1930 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2888 1163
Size: 573 1152 4213
Fragments arranged by size:
4213 1152 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3388 4149 5177 1540
Size: 275 186 1387 761 1028 2301
Fragments arranged by size:
2301 1387 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3999 4084 5155 3999
Size: 85 1071 4782
Fragments arranged by size:
4782 1071 85
BsmI GAATG_Cn'
Cuts at: 3449 3542 3449
Size: 93 5845
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2051 2431 2753 3257 3999 4084 5155
Size: 399 261 380 322 504 742 85 1071
Cuts at: 5155 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 504 399 380 322 261
85
BspEI T'CCGG_A
Cuts at: 2190 5601 2190
Size: 3411 2527
BspHI T'CATG_A
Cuts at: 3827 5557 3827
Size: 1730 4208
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2175 2388 3092 3293 844
Size: 1331 213 704 201 3489
Fragments arranged by size:
3489 1331 704 213 201
BsrBI CCG'CTC
Cuts at: 1394 1823 2028 2957 3825 1394
Size: 429 205 929 868 3507
Fragments arranged by size:
3507 929 868 429 205
BsrDI GCAATG_nn'
Cuts at: 65 1248 4433 4607 65
Size: 1183 3185 174 1396
Fragments arranged by size:
3185 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2104 4582 4769 1700
Size: 110 294 2478 187 2869
Fragments arranged by size:
2869 2478 294 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5938
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5938
BssSI C'ACGA_G
Cuts at: 3998 5010 3998
Size: 1012 4926
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5938
BstBI TT'CG_AA
Cuts at: 2007 4742 2007
Size: 2735 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5938
BstXI CCAn_nnnn'nTGG
Cuts at: 2446 2446
Size: 5938
BstYI r'GATC_y
Cuts at: 1972 2907 3268 3635 4020 4037 5489 5575 5587
Size: 935 361 367 385 17 1452 86 12
Cuts at: 5587 5933 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 935 385 367 361 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2930 2930
Size: 5938
BtgI C'CryG_G
Cuts at: 513 1065 2878 513
Size: 552 1813 3573
Fragments arranged by size:
3573 1813 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5199
Fragments arranged by size:
5199 683 56
BtsI GCAGTG_nn'
Cuts at: 3094 3454 4248 4268 3094
Size: 360 794 20 4764
Fragments arranged by size:
4764 794 360 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5938
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5903
DraI TTT'AAA
Cuts at: 3242 3242
Size: 5938
DraIII CAC_nnn'GTG
Cuts at: 2866 2896 2866
Size: 30 5908
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4945 809
Size: 507 3629 1802
Fragments arranged by size:
3629 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2070 3385 4274
Size: 54 1425 50 275 18 240 1315 889
Cuts at: 4274 5903 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1315 889 275 240 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3385 1537
Size: 275 1573 4090
Fragments arranged by size:
4090 1573 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2918 3189 3868 1353
Size: 1565 271 679 3423
Fragments arranged by size:
3423 1565 679 271
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4525 4899 5045 387
Size: 1064 138 2936 374 146 1280
Fragments arranged by size:
2936 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2235 2600 3002 3087 4001 4598 5385
Size: 393 603 365 402 85 914 597 787
Cuts at: 5385 5653 1239
Size: 268 1524
Fragments arranged by size:
1524 914 787 603 597 402 393 365
268 85
EcoNI CCTnn'n_nnAGG
Cuts at: 2651 2651
Size: 5938
EcoO109I rG'GnC_Cy
Cuts at: 2377 3075 3169 2377
Size: 698 94 5146
Fragments arranged by size:
5146 698 94
EcoRI G'AATT_C
Cuts at: 3247 3625 3247
Size: 378 5560
FspI TGC'GCA
Cuts at: 4444 4444
Size: 5938
HaeII r_GCGC'y
Cuts at: 1678 5085 1678
Size: 3407 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3173 3205 4579 4611 4653
Size: 32 135 32 1601 32 1374 32 42
Cuts at: 4653 4685 1373
Size: 32 2626
Fragments arranged by size:
2626 1601 1374 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3282 3528 669
Size: 988 281 1344 246 3079
Fragments arranged by size:
3079 1344 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2158 748
Size: 1410 4528
HpaI GTT'AAC
Cuts at: 3528 3528
Size: 5938
KpnI G_GTAC'C
Cuts at: 3263 3263
Size: 5938
MfeI C'AATT_G
Cuts at: 3537 3537
Size: 5938
MluI A'CGCG_T
Cuts at: 5814 5814
Size: 5938
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2157 2287 5052 5236 1305
Size: 852 130 2765 184 2007
Fragments arranged by size:
2765 2007 852 184 130
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2072 10
Size: 54 1425 343 240 3876
Fragments arranged by size:
3876 1425 343 240 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3896 4255 4414 518
Size: 648 2730 359 159 2042
Fragments arranged by size:
2730 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3007 3211 3354 4031 5179 5424 1606
Size: 1401 204 143 677 1148 245 2120
Fragments arranged by size:
2120 1401 1148 677 245 204 143
NaeI GCC'GGC
Cuts at: 2106 2106
Size: 5938
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5386
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5938
NgoMIV G'CCGG_C
Cuts at: 2104 2104
Size: 5938
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5938
NotI GC'GGCC_GC
Cuts at: 3385 3385
Size: 5938
NspI r_CATG'y
Cuts at: 1132 1229 2201 2554 3296 4841 1132
Size: 97 972 353 742 1545 2229
Fragments arranged by size:
2229 1545 972 742 353 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5938
PciI A'CATG_T
Cuts at: 4837 4837
Size: 5938
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2896 1108
Size: 1788 4150
PfoI T'CCnGG_A
Cuts at: 2091 2091
Size: 5938
PmeI GTTT'AAAC
Cuts at: 3242 3242
Size: 5938
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4007 4039 5520 5552 4007
Size: 32 1481 32 4393
Fragments arranged by size:
4393 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2377 3075 3169 2377
Size: 698 94 5146
Fragments arranged by size:
5146 698 94
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5938
PsiI TTA'TAA
Cuts at: 3508 3508
Size: 5938
PstI C_TGCA'G
Cuts at: 830 2172 3089 3290 830
Size: 1342 917 201 3478
Fragments arranged by size:
3478 1342 917 201
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5938
PvuII CAG'CTG
Cuts at: 3211 3211
Size: 5938
SalI G'TCGA_C
Cuts at: 1936 3280 1936
Size: 1344 4594
SanDI GG'GwC_CC
Cuts at: 3075 3075
Size: 5938
SbfI CC_TGCA'GG
Cuts at: 3290 3290
Size: 5938
ScaI AGT'ACT
Cuts at: 4186 5898 4186
Size: 1712 4226
SexAI A'CCwGG_T
Cuts at: 2727 2838 3057 2727
Size: 111 219 5608
Fragments arranged by size:
5608 219 111
SfcI C'TryA_G
Cuts at: 826 1045 2168 3085 3127 3286 4421 5102 5293
Size: 219 1123 917 42 159 1135 681 191
Cuts at: 5293 826
Size: 1471
Fragments arranged by size:
1471 1135 1123 917 681 219 191 159
42
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5938
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5938
SmaI CCC'GGG
Cuts at: 1872 3265 1872
Size: 1393 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2501 4042 4943 5205 820
Size: 197 926 558 1541 901 262 1553
Fragments arranged by size:
1553 1541 926 901 558 262 197
SnaBI TAC'GTA
Cuts at: 493 3235 3729 493
Size: 2742 494 2702
Fragments arranged by size:
2742 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5938
SphI G_CATG'C
Cuts at: 1132 3296 1132
Size: 2164 3774
SspI AAT'ATT
Cuts at: 5 52 3862 5
Size: 47 3810 2081
Fragments arranged by size:
3810 2081 47
StuI AGG'CCT
Cuts at: 1517 2531 1517
Size: 1014 4924
StyI C'CwwG_G
Cuts at: 513 1065 2346 3377 513
Size: 552 1281 1031 3074
Fragments arranged by size:
3074 1281 1031 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3965 3982 4135 4320 4765 325
Size: 1255 2385 17 153 185 445 1498
Fragments arranged by size:
2385 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4184 5896 96
Size: 274 80 33 51 1443 2207 1712 138
Fragments arranged by size:
2207 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5898 5930 5898
Size: 32 5906
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5938
XbaI T'CTAG_A
Cuts at: 3274 3274
Size: 5938
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5938
XmaI C'CCGG_G
Cuts at: 1870 3263 1870
Size: 1393 4545
XmnI GAAnn'nnTTC
Cuts at: 4067 4067
Size: 5938
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3746 276
Size: 53 83 186 936 2212 2468
Fragments arranged by size:
2468 2212 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SanDI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I EcoICRI EcoRV FalI FseI FspAI
KasI NarI NruI NsiI PacI PmlI PspOMI PspXI
PsrI RsrII SacI SacII SapI SfiI SfoI SrfI
SwaI XcmI