Restriction map for
pFN21ASDA1171
(Circular) (Six-base) MAPSORT of: pFN21ASDA1171.seq Check: 5343 from: 1 to: 6406
pFN21ASDA1171 6406 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2311 2311
Size: 6406
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2715 4216 278
Size: 53 83 186 936 1179 1501 2468
Fragments arranged by size:
2468 1501 1179 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3749 1937
Size: 1812 4594
Acc65I G'GTAC_C
Cuts at: 3727 3727
Size: 6406
AclI AA'CG_TT
Cuts at: 1563 4533 4906 1563
Size: 2970 373 3063
Fragments arranged by size:
3063 2970 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2147 2975 4469 5853 6121 1632
Size: 515 828 1494 1384 268 1917
Fragments arranged by size:
1917 1494 1384 828 515 268
AfeI AGC'GCT
Cuts at: 2663 3101 3344 2663
Size: 438 243 5725
Fragments arranged by size:
5725 438 243
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6209
AflIII A'CryG_T
Cuts at: 1203 5305 6282 1203
Size: 4102 977 1327
Fragments arranged by size:
4102 1327 977
AgeI A'CCGG_T
Cuts at: 5237 5237
Size: 6406
AhdI GACnn_n'nnGTC
Cuts at: 3082 5135 3082
Size: 2053 4353
AleI CACnn'nnGTG
Cuts at: 1166 2321 1166
Size: 1155 5251
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2132 2306 2664 1184
Size: 948 174 358 4926
Fragments arranged by size:
4926 948 358 174
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2873 2905 2873
Size: 32 6374
AlwNI CAG_nnn'CTG
Cuts at: 2086 2125 2194 2286 2334 2800 2929 3534 4149
Size: 39 69 92 48 466 129 605 615
Cuts at: 4149 5721 2086
Size: 1572 2771
Fragments arranged by size:
2771 1572 615 605 466 129 92 69
48 39
ApaI G_GGCC'C
Cuts at: 2094 2158 2094
Size: 64 6342
ApaLI G'TGCA_C
Cuts at: 4463 5619 4463
Size: 1156 5250
ApoI r'AATT_y
Cuts at: 1493 3715 3941 4093 6202 1493
Size: 2222 226 152 2109 1697
Fragments arranged by size:
2222 2109 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2138 2138
Size: 6406
AseI AT'TA_AT
Cuts at: 160 1032 4960 160
Size: 872 3928 1606
Fragments arranged by size:
3928 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6406
AvaI C'yCGr_G
Cuts at: 1870 1943 2300 2700 3095 3371 3731 1870
Size: 73 357 400 395 276 360 4545
Fragments arranged by size:
4545 400 395 360 357 276 73
BamHI G'GATC_C
Cuts at: 3736 4103 3736
Size: 367 6039
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2323 3727 5182 618
Size: 325 217 627 536 1404 1455 1842
Fragments arranged by size:
1842 1455 1404 627 536 325 217
BanII G_rGCy'C
Cuts at: 1391 2094 2158 3411 3725 1391
Size: 703 64 1253 314 4072
Fragments arranged by size:
4072 1253 703 314 64
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2707 2861 928
Size: 971 808 154 4473
Fragments arranged by size:
4473 971 808 154
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3086 3120 4597 4631 1903
Size: 34 1149 34 1477 34 3678
Fragments arranged by size:
3678 1477 1149 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2030 4298 5508 2030
Size: 2268 1210 2928
Fragments arranged by size:
2928 2268 1210
BclI T'GATC_A
Cuts at: 1548 3360 1548
Size: 1812 4594
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2426 2435 3668
Size: 107 122 71 631 605 754 9 1233
Cuts at: 3668 5017 136
Size: 1349 1525
Fragments arranged by size:
1525 1349 1233 754 631 605 122 107
71 9
BglII A'GATC_T
Cuts at: 6401 6401
Size: 6406
BlpI GC'TnA_GC
Cuts at: 2102 3823 2102
Size: 1721 4685
Bme1580I G_kGCm'C
Cuts at: 1790 2094 2158 2328 4467 5623 1790
Size: 304 64 170 2139 1156 2573
Fragments arranged by size:
2573 2139 1156 304 170 64
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6406
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2161 3296 3691
Size: 416 53 83 399 24 744 1135 395
Cuts at: 3691 4137 5095 442
Size: 446 958 1753
Fragments arranged by size:
1753 1135 958 744 446 416 399 395
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6406
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2016 2822 2900 3277 5066 1239
Size: 777 806 78 377 1789 2579
Fragments arranged by size:
2579 1789 806 777 377 78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2750 4531 5396 5694 2750
Size: 1781 865 298 3462
Fragments arranged by size:
3462 1781 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5069 882
Size: 617 3570 2219
Fragments arranged by size:
3570 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3703 4197 493
Size: 711 2499 494 2702
Fragments arranged by size:
2702 2499 711 494
BsaBI GATnn'nnATC
Cuts at: 6400 6400
Size: 6406
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2232 2712 4213 4595
Size: 53 83 186 936 699 480 1501 382
Cuts at: 4595 5230 275
Size: 635 1451
Fragments arranged by size:
1501 1451 936 699 635 480 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3302 4839 5237 5511 5658 6069 3302
Size: 1537 398 274 147 411 3639
Fragments arranged by size:
3639 1537 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2873 2903 3419 3449 3479
Size: 30 165 30 1470 30 516 30 30
Cuts at: 3479 3509 1178
Size: 30 4075
Fragments arranged by size:
4075 1470 516 165 30 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3124 3427 3567 1186
Size: 1938 303 140 4025
Fragments arranged by size:
4025 1938 303 140
BseYI C'CCAG_C
Cuts at: 2327 5609 6105 2327
Size: 3282 496 2628
Fragments arranged by size:
3282 2628 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2092 2432 3359 1163
Size: 573 356 340 927 4210
Fragments arranged by size:
4210 927 573 356 340
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2430 3856 4617 5645 1540
Size: 275 186 429 1426 761 1028 2301
Fragments arranged by size:
2301 1426 1028 761 429 275 186
BsiHKAI G_wGCw'C
Cuts at: 4467 4552 5623 4467
Size: 85 1071 5250
Fragments arranged by size:
5250 1071 85
BsmI GAATG_Cn'
Cuts at: 2347 3314 3917 4010 2347
Size: 967 603 93 4743
Fragments arranged by size:
4743 967 603 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2094 2158 2328 3411 3725 4467 4552
Size: 399 304 64 170 1083 314 742 85
Cuts at: 4552 5623 1391
Size: 1071 2174
Fragments arranged by size:
2174 1083 1071 742 399 314 304 170
85 64
BspEI T'CCGG_A
Cuts at: 6069 6069
Size: 6406
BspHI T'CATG_A
Cuts at: 4295 6025 4295
Size: 1730 4676
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2311 2632 3498 3761 844
Size: 1467 321 866 263 3489
Fragments arranged by size:
3489 1467 866 321 263
BsrBI CCG'CTC
Cuts at: 1394 1823 3045 3476 4293 1394
Size: 429 1222 431 817 3507
Fragments arranged by size:
3507 1222 817 431 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2512 3654 4901 5075 65
Size: 1183 1264 1142 1247 174 1396
Fragments arranged by size:
1396 1264 1247 1183 1142 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2425 5050 5237 1700
Size: 110 615 2625 187 2869
Fragments arranged by size:
2869 2625 615 187 110
BsrGI T'GTAC_A
Cuts at: 96 2241 3013 96
Size: 2145 772 3489
Fragments arranged by size:
3489 2145 772
BssHII G'CGCG_C
Cuts at: 1925 2138 1925
Size: 213 6193
BssSI C'ACGA_G
Cuts at: 4466 5478 4466
Size: 1012 5394
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2286 3534 794
Size: 1492 1248 3666
Fragments arranged by size:
3666 1492 1248
BstBI TT'CG_AA
Cuts at: 2007 5210 2007
Size: 3203 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6406
BstXI CCAn_nnnn'nTGG
Cuts at: 3517 3517
Size: 6406
BstYI r'GATC_y
Cuts at: 1972 2370 2919 3736 4103 4488 4505 5957 6043
Size: 398 549 817 367 385 17 1452 86
Cuts at: 6043 6055 6401 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 817 549 398 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3333 3333
Size: 6406
BtgI C'CryG_G
Cuts at: 513 1065 2022 2430 3663 513
Size: 552 957 408 1233 3256
Fragments arranged by size:
3256 1233 957 552 408
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2833 487
Size: 683 56 1607 4060
Fragments arranged by size:
4060 1607 683 56
BtsI GCAGTG_nn'
Cuts at: 3607 3922 4716 4736 3607
Size: 315 794 20 5277
Fragments arranged by size:
5277 794 315 20
ClaI AT'CG_AT
Cuts at: 1554 3576 1554
Size: 2022 4384
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6371
DraI TTT'AAA
Cuts at: 3710 3710
Size: 6406
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5413 809
Size: 507 4097 1802
Fragments arranged by size:
4097 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2385 2427 2619
Size: 54 1425 50 275 18 555 42 192
Cuts at: 2619 2757 2937 3021 3159 3471 3559 3853 4742
Size: 138 180 84 138 312 88 294 889
Cuts at: 4742 6371 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 555 312 294 275 192
180 138 138 88 84 54 50 43
42 18
EagI C'GGCC_G
Cuts at: 1537 1812 2427 3853 1537
Size: 275 615 1426 4090
Fragments arranged by size:
4090 1426 615 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2978 4336 1353
Size: 1625 1358 3423
Fragments arranged by size:
3423 1625 1358
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2888 3421 4993 5367 5513 387
Size: 1064 138 1299 533 1572 374 146 1280
Fragments arranged by size:
1572 1299 1280 1064 533 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2016 2147 2822 2900 2975 3277 4469
Size: 393 384 131 675 78 75 302 1192
Cuts at: 4469 5066 5853 6121 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1192 787 675 597 393 384 302
268 131 78 75
EcoNI CCTnn'n_nnAGG
Cuts at: 2723 2723
Size: 6406
EcoO109I rG'GnC_Cy
Cuts at: 2083 2090 2575 3091 3583 3685 2083
Size: 7 485 516 492 102 4804
Fragments arranged by size:
4804 516 492 485 102 7
EcoRI G'AATT_C
Cuts at: 3715 4093 3715
Size: 378 6028
FspI TGC'GCA
Cuts at: 2366 4912 2366
Size: 2546 3860
HaeII r_GCGC'y
Cuts at: 1678 2194 2665 2756 3103 3346 3569 5553 1678
Size: 516 471 91 347 243 223 1984 2531
Fragments arranged by size:
2531 1984 516 471 347 243 223 91
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3068 3100 3443 3475 5047
Size: 32 135 32 1496 32 343 32 1572
Cuts at: 5047 5079 5121 5153 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1572 1496 343 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3750 3996 669
Size: 988 281 1812 246 3079
Fragments arranged by size:
3079 1812 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6406
HpaI GTT'AAC
Cuts at: 3996 3996
Size: 6406
KpnI G_GTAC'C
Cuts at: 3731 3731
Size: 6406
MfeI C'AATT_G
Cuts at: 4005 4005
Size: 6406
MluI A'CGCG_T
Cuts at: 6282 6282
Size: 6406
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2607 2787 5520 5704 1305
Size: 1302 180 2733 184 2007
Fragments arranged by size:
2733 2007 1302 184 180
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2387 2759 2939 3161 10
Size: 54 1425 343 555 372 180 222 3255
Fragments arranged by size:
3255 1425 555 372 343 222 180 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2321 2972 3515 4364 4723 4882 518
Size: 648 1155 651 543 849 359 159 2042
Fragments arranged by size:
2042 1155 849 651 648 543 359 159
MspA1I CmG'CkG
Cuts at: 1606 2134 2286 2296 2800 3449 3822 4499 5647
Size: 528 152 10 504 649 373 677 1148
Cuts at: 5647 5892 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 649 528 504 373 245
152 10
NaeI GCC'GGC
Cuts at: 2427 2427
Size: 6406
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4897
Fragments arranged by size:
4897 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6406
NgoMIV G'CCGG_C
Cuts at: 2425 2425
Size: 6406
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6406
NotI GC'GGCC_GC
Cuts at: 3853 3853
Size: 6406
NruI TCG'CGA
Cuts at: 2852 2852
Size: 6406
NspI r_CATG'y
Cuts at: 1132 1229 3764 5309 1132
Size: 97 2535 1545 2229
Fragments arranged by size:
2535 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6406
PciI A'CATG_T
Cuts at: 5305 5305
Size: 6406
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2086 3255 1108
Size: 978 1169 4259
Fragments arranged by size:
4259 1169 978
PfoI T'CCnGG_A
Cuts at: 2032 2079 2199 3681 2032
Size: 47 120 1482 4757
Fragments arranged by size:
4757 1482 120 47
PmeI GTTT'AAAC
Cuts at: 3710 3710
Size: 6406
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2873 2905 3029 3061 3497 3529 4475 4507 5988
Size: 32 124 32 436 32 946 32 1481
Cuts at: 5988 6020 2873
Size: 32 3259
Fragments arranged by size:
3259 1481 946 436 124 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 2083 2575 3091 3583 3685 2083
Size: 492 516 492 102 4804
Fragments arranged by size:
4804 516 492 492 102
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6406
PsiI TTA'TAA
Cuts at: 3976 3976
Size: 6406
PspOMI G'GGCC_C
Cuts at: 2090 2154 2090
Size: 64 6342
PstI C_TGCA'G
Cuts at: 830 2121 2643 2934 3144 3330 3758 830
Size: 1291 522 291 210 186 428 3478
Fragments arranged by size:
3478 1291 522 428 291 210 186
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6406
PvuII CAG'CTG
Cuts at: 2134 2286 2134
Size: 152 6254
SalI G'TCGA_C
Cuts at: 1936 3748 1936
Size: 1812 4594
SanDI GG'GwC_CC
Cuts at: 3091 3091
Size: 6406
SbfI CC_TGCA'GG
Cuts at: 2643 3758 2643
Size: 1115 5291
ScaI AGT'ACT
Cuts at: 2591 4654 6366 2591
Size: 2063 1712 2631
Fragments arranged by size:
2631 2063 1712
SexAI A'CCwGG_T
Cuts at: 2577 2577
Size: 6406
SfcI C'TryA_G
Cuts at: 826 1045 2117 2452 2639 2930 3140 3326 3754
Size: 219 1072 335 187 291 210 186 428
Cuts at: 3754 4889 5570 5761 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1072 681 428 335 291 219
210 191 187 186
SfiI GGCCn_nnn'nGGCC
Cuts at: 2435 2435
Size: 6406
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6406
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6406
SmaI CCC'GGG
Cuts at: 1872 2702 3733 1872
Size: 830 1031 4545
Fragments arranged by size:
4545 1031 830
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2765 4510 5411 5673 820
Size: 197 926 822 1745 901 262 1553
Fragments arranged by size:
1745 1553 926 901 822 262 197
SnaBI TAC'GTA
Cuts at: 493 3703 4197 493
Size: 3210 494 2702
Fragments arranged by size:
3210 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6406
SphI G_CATG'C
Cuts at: 1132 3764 1132
Size: 2632 3774
SspI AAT'ATT
Cuts at: 5 52 4330 5
Size: 47 4278 2081
Fragments arranged by size:
4278 2081 47
StuI AGG'CCT
Cuts at: 1517 2486 3671 1517
Size: 969 1185 4252
Fragments arranged by size:
4252 1185 969
StyI C'CwwG_G
Cuts at: 513 1065 2022 2094 3845 513
Size: 552 957 72 1751 3074
Fragments arranged by size:
3074 1751 957 552 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3343 3518 3710 4433 4450 4603 4788
Size: 1255 1763 175 192 723 17 153 185
Cuts at: 4788 5233 325
Size: 445 1498
Fragments arranged by size:
1763 1498 1255 723 445 192 185 175
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2241 2589 3013
Size: 274 80 33 51 1443 264 348 424
Cuts at: 3013 4652 6364 96
Size: 1639 1712 138
Fragments arranged by size:
1712 1639 1443 424 348 274 264 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3419 3451 6366 6398 3419
Size: 32 2915 32 3427
Fragments arranged by size:
3427 2915 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2860 3505 1316
Size: 1544 645 4217
Fragments arranged by size:
4217 1544 645
XbaI T'CTAG_A
Cuts at: 3742 3742
Size: 6406
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2083 2083
Size: 6406
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6406
XmaI C'CCGG_G
Cuts at: 1870 2700 3731 1870
Size: 830 1031 4545
Fragments arranged by size:
4545 1031 830
XmnI GAAnn'nnTTC
Cuts at: 2888 4535 2888
Size: 1647 4759
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2713 4214 276
Size: 53 83 186 936 1179 1501 2468
Fragments arranged by size:
2468 1501 1179 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI
BanII BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NruI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI
BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI
KasI NarI NsiI PacI PmlI PspXI PsrI RsrII
SacI SacII SapI SfoI SrfI SwaI