Restriction map for
pFN21ASDA1158
(Circular) (Six-base) MAPSORT of: pFN21ASDA1158.seq Check: 33 from: 1 to: 5374
pFN21ASDA1158 5374 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2387 2387
Size: 5374
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3184 278
Size: 53 83 186 936 1648 2468
Fragments arranged by size:
2468 1648 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2717 1937
Size: 780 4594
Acc65I G'GTAC_C
Cuts at: 2653 2695 2653
Size: 42 5332
AclI AA'CG_TT
Cuts at: 1563 3501 3874 1563
Size: 1938 373 3063
Fragments arranged by size:
3063 1938 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3437 4821 5089 1632
Size: 1805 1384 268 1917
Fragments arranged by size:
1917 1805 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5177
AflIII A'CryG_T
Cuts at: 1203 2328 2604 4273 5250 1203
Size: 1125 276 1669 977 1327
Fragments arranged by size:
1669 1327 1125 977 276
AgeI A'CCGG_T
Cuts at: 4205 4205
Size: 5374
AhdI GACnn_n'nnGTC
Cuts at: 4103 4103
Size: 5374
AleI CACnn'nnGTG
Cuts at: 1166 2195 2198 2486 1166
Size: 1029 3 288 4054
Fragments arranged by size:
4054 1029 288 3
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2336 1184
Size: 1152 4222
AlwNI CAG_nnn'CTG
Cuts at: 2085 3117 4689 2085
Size: 1032 1572 2770
Fragments arranged by size:
2770 1572 1032
ApaLI G'TGCA_C
Cuts at: 3431 4587 3431
Size: 1156 4218
ApoI r'AATT_y
Cuts at: 1493 2683 2909 3061 5170 1493
Size: 1190 226 152 2109 1697
Fragments arranged by size:
2109 1697 1190 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3928 160
Size: 872 2896 1606
Fragments arranged by size:
2896 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5374
AvaI C'yCGr_G
Cuts at: 1870 1943 2219 2391 2699 1870
Size: 73 276 172 308 4545
Fragments arranged by size:
4545 308 276 172 73
BamHI G'GATC_C
Cuts at: 2704 3071 2704
Size: 367 5007
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2292 2653 2695 4150 618
Size: 325 217 627 505 361 42 1455 1842
Fragments arranged by size:
1842 1455 627 505 361 325 217 42
BanII G_rGCy'C
Cuts at: 1391 2282 2693 1391
Size: 891 411 4072
Fragments arranged by size:
4072 891 411
BbeI G_GCGC'C
Cuts at: 2296 2296
Size: 5374
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2435 2515 928
Size: 971 536 80 3787
Fragments arranged by size:
3787 971 536 80
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3565 3599 1903
Size: 34 1628 34 3678
Fragments arranged by size:
3678 1628 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3266 4476 3266
Size: 1210 4164
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5374
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2588 3985 136
Size: 107 122 71 631 605 916 1397 1525
Fragments arranged by size:
1525 1397 916 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5369 5369
Size: 5374
BlpI GC'TnA_GC
Cuts at: 2791 2791
Size: 5374
Bme1580I G_kGCm'C
Cuts at: 1790 2112 3435 4591 1790
Size: 322 1323 1156 2573
Fragments arranged by size:
2573 1323 1156 322
BmgBI CAC'GTC
Cuts at: 1318 2329 1318
Size: 1011 4363
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2083 3105 4063
Size: 416 53 83 399 24 666 1022 958
Cuts at: 4063 442
Size: 1753
Fragments arranged by size:
1753 1022 958 666 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5374
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2490 4034 1239
Size: 1251 1544 2579
Fragments arranged by size:
2579 1544 1251
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3499 4364 4662 3499
Size: 865 298 4211
Fragments arranged by size:
4211 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2497 2578 4037 882
Size: 617 998 81 1459 2219
Fragments arranged by size:
2219 1459 998 617 81
BsaAI yAC'GTr
Cuts at: 493 1204 2671 3165 493
Size: 711 1467 494 2702
Fragments arranged by size:
2702 1467 711 494
BsaBI GATnn'nnATC
Cuts at: 2510 5368 2510
Size: 2858 2516
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2293 3181 3563 4198
Size: 53 83 186 936 760 888 382 635
Cuts at: 4198 275
Size: 1451
Fragments arranged by size:
1451 936 888 760 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3807 4205 4479 4626 5037 3807
Size: 398 274 147 411 4144
Fragments arranged by size:
4144 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2387 2417 1178
Size: 30 165 30 984 30 4135
Fragments arranged by size:
4135 984 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2192 2477 1186
Size: 1006 285 4083
Fragments arranged by size:
4083 1006 285
BseYI C'CCAG_C
Cuts at: 4577 5073 4577
Size: 496 4878
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2150 1163
Size: 573 414 4387
Fragments arranged by size:
4387 573 414
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2824 3585 4613 1540
Size: 275 186 823 761 1028 2301
Fragments arranged by size:
2301 1028 823 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2346 3435 3520 4591 2346
Size: 1089 85 1071 3129
Fragments arranged by size:
3129 1089 1071 85
BsmI GAATG_Cn'
Cuts at: 2885 2978 2885
Size: 93 5281
BsmBI CGTCTCn'nnnn_
Cuts at: 2111 2111
Size: 5374
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2112 2282 2346 2693 3435 3520 4591
Size: 399 322 170 64 347 742 85 1071
Cuts at: 4591 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 347 322 170 85
64
BspEI T'CCGG_A
Cuts at: 5037 5037
Size: 5374
BspHI T'CATG_A
Cuts at: 2502 3263 4993 2502
Size: 761 1730 2883
Fragments arranged by size:
2883 1730 761
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2328 2387 2729 844
Size: 1484 59 342 3489
Fragments arranged by size:
3489 1484 342 59
BsrBI CCG'CTC
Cuts at: 1394 1823 3261 1394
Size: 429 1438 3507
Fragments arranged by size:
3507 1438 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2389 3869 4043 65
Size: 1183 1141 1480 174 1396
Fragments arranged by size:
1480 1396 1183 1141 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4018 4205 1700
Size: 110 2208 187 2869
Fragments arranged by size:
2869 2208 187 110
BsrGI T'GTAC_A
Cuts at: 96 2508 96
Size: 2412 2962
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5374
BssSI C'ACGA_G
Cuts at: 2060 3434 4446 2060
Size: 1374 1012 2988
Fragments arranged by size:
2988 1374 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2163 794
Size: 1369 4005
BstBI TT'CG_AA
Cuts at: 2007 4178 2007
Size: 2171 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5374
BstXI CCAn_nnnn'nTGG
Cuts at: 2197 2200 2197
Size: 3 5371
BstYI r'GATC_y
Cuts at: 1972 2704 3071 3456 3473 4925 5011 5023 5369
Size: 732 367 385 17 1452 86 12 346
Cuts at: 5369 1972
Size: 1977
Fragments arranged by size:
1977 1452 732 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2193 513
Size: 552 1128 3694
Fragments arranged by size:
3694 1128 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4635
Fragments arranged by size:
4635 683 56
BtsI GCAGTG_nn'
Cuts at: 2306 2890 3684 3704 2306
Size: 584 794 20 3976
Fragments arranged by size:
3976 794 584 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5374
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5339
DraI TTT'AAA
Cuts at: 2678 2678
Size: 5374
DraIII CAC_nnn'GTG
Cuts at: 2535 2535
Size: 5374
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4381 809
Size: 507 3065 1802
Fragments arranged by size:
3065 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2260 2821 3710
Size: 54 1425 50 275 18 430 561 889
Cuts at: 3710 5339 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 561 430 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2821 1537
Size: 275 1009 4090
Fragments arranged by size:
4090 1009 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2165 2580 3304 1353
Size: 812 415 724 3423
Fragments arranged by size:
3423 812 724 415
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3961 4335 4481 387
Size: 1064 138 2372 374 146 1280
Fragments arranged by size:
2372 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2490 3437 4034 4821 5089 1239
Size: 393 858 947 597 787 268 1524
Fragments arranged by size:
1524 947 858 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2317 2317
Size: 5374
EcoRI G'AATT_C
Cuts at: 2683 3061 2683
Size: 378 4996
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2255 2255
Size: 5374
FspI TGC'GCA
Cuts at: 3880 3880
Size: 5374
HaeII r_GCGC'y
Cuts at: 1678 2296 4521 1678
Size: 618 2225 2531
Fragments arranged by size:
2531 2225 618
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2468 2500 4015 4047 4089
Size: 32 135 32 896 32 1515 32 42
Cuts at: 4089 4121 1373
Size: 32 2626
Fragments arranged by size:
2626 1515 896 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2718 2964 669
Size: 988 281 780 246 3079
Fragments arranged by size:
3079 988 780 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5374
HpaI GTT'AAC
Cuts at: 2964 2964
Size: 5374
KasI G'GCGC_C
Cuts at: 2292 2292
Size: 5374
KpnI G_GTAC'C
Cuts at: 2657 2699 2657
Size: 42 5332
MfeI C'AATT_G
Cuts at: 2973 2973
Size: 5374
MluI A'CGCG_T
Cuts at: 2604 5250 2604
Size: 2646 2728
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4488 4672 1305
Size: 3183 184 2007
Fragments arranged by size:
3183 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2262 10
Size: 54 1425 343 430 3122
Fragments arranged by size:
3122 1425 430 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2195 2198 2339 2486 2549 3332 3691
Size: 648 1029 3 141 147 63 783 359
Cuts at: 3691 3850 518
Size: 159 2042
Fragments arranged by size:
2042 1029 783 648 359 159 147 141
63 3
MspA1I CmG'CkG
Cuts at: 1606 2790 3467 4615 4860 1606
Size: 1184 677 1148 245 2120
Fragments arranged by size:
2120 1184 1148 677 245
NarI GG'CG_CC
Cuts at: 2293 2293
Size: 5374
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4822
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5374
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5374
NotI GC'GGCC_GC
Cuts at: 2821 2821
Size: 5374
NspI r_CATG'y
Cuts at: 1132 1229 2732 4277 1132
Size: 97 1503 1545 2229
Fragments arranged by size:
2229 1545 1503 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5374
PciI A'CATG_T
Cuts at: 4273 4273
Size: 5374
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2272 1108
Size: 1164 4210
PmeI GTTT'AAAC
Cuts at: 2678 2678
Size: 5374
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3443 3475 4956 4988 3443
Size: 32 1481 32 3829
Fragments arranged by size:
3829 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2317 2317
Size: 5374
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5374
PsiI TTA'TAA
Cuts at: 2944 2944
Size: 5374
PstI C_TGCA'G
Cuts at: 830 2301 2325 2726 830
Size: 1471 24 401 3478
Fragments arranged by size:
3478 1471 401 24
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5374
SalI G'TCGA_C
Cuts at: 1936 2716 1936
Size: 780 4594
SbfI CC_TGCA'GG
Cuts at: 2325 2726 2325
Size: 401 4973
ScaI AGT'ACT
Cuts at: 3622 5334 3622
Size: 1712 3662
SfcI C'TryA_G
Cuts at: 826 1045 2212 2297 2321 2563 2722 3857 4538
Size: 219 1167 85 24 242 159 1135 681
Cuts at: 4538 4729 826
Size: 191 1471
Fragments arranged by size:
1471 1167 1135 681 242 219 191 159
85 24
SfoI GGC'GCC
Cuts at: 2294 2294
Size: 5374
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5374
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5374
SmaI CCC'GGG
Cuts at: 1872 2393 2701 1872
Size: 521 308 4545
Fragments arranged by size:
4545 521 308
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3478 4379 4641 820
Size: 197 926 1535 901 262 1553
Fragments arranged by size:
1553 1535 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2671 3165 493
Size: 2178 494 2702
Fragments arranged by size:
2702 2178 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5374
SphI G_CATG'C
Cuts at: 1132 2732 1132
Size: 1600 3774
SspI AAT'ATT
Cuts at: 5 52 3298 5
Size: 47 3246 2081
Fragments arranged by size:
3246 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5374
StyI C'CwwG_G
Cuts at: 513 1065 2813 513
Size: 552 1748 3074
Fragments arranged by size:
3074 1748 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3401 3418 3571 3756 4201 325
Size: 1255 1821 17 153 185 445 1498
Fragments arranged by size:
1821 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2508 3620 5332
Size: 274 80 33 51 1443 531 1112 1712
Cuts at: 5332 96
Size: 138
Fragments arranged by size:
1712 1443 1112 531 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5334 5366 5334
Size: 32 5342
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5374
XbaI T'CTAG_A
Cuts at: 2710 2710
Size: 5374
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2197 2197
Size: 5374
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5374
XmaI C'CCGG_G
Cuts at: 1870 2391 2699 1870
Size: 521 308 4545
Fragments arranged by size:
4545 521 308
XmnI GAAnn'nnTTC
Cuts at: 3503 3503
Size: 5374
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3182 276
Size: 53 83 186 936 1648 2468
Fragments arranged by size:
2468 1648 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SrfI SwaI