Restriction map for
pFN21ASDA1152
(Circular) (Six-base) MAPSORT of: pFN21ASDA1152.seq Check: 3687 from: 1 to: 5782
pFN21ASDA1152 5782 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3592 278
Size: 53 83 186 936 2056 2468
Fragments arranged by size:
2468 2056 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2392 2950 3125 1937
Size: 455 558 175 4594
Fragments arranged by size:
4594 558 455 175
Acc65I G'GTAC_C
Cuts at: 2928 3103 2928
Size: 175 5607
AclI AA'CG_TT
Cuts at: 1563 3909 4282 1563
Size: 2346 373 3063
Fragments arranged by size:
3063 2346 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2435 2483 2868 3845 5229 5497 1632
Size: 803 48 385 977 1384 268 1917
Fragments arranged by size:
1917 1384 977 803 385 268 48
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5585
AflIII A'CryG_T
Cuts at: 1203 2673 4681 5658 1203
Size: 1470 2008 977 1327
Fragments arranged by size:
2008 1470 1327 977
AgeI A'CCGG_T
Cuts at: 4613 4613
Size: 5782
AhdI GACnn_n'nnGTC
Cuts at: 4511 4511
Size: 5782
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5782
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2986 1184
Size: 1802 3980
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2149 2181 2149
Size: 32 5750
AlwNI CAG_nnn'CTG
Cuts at: 2461 2524 3525 5097 2461
Size: 63 1001 1572 3146
Fragments arranged by size:
3146 1572 1001 63
ApaLI G'TGCA_C
Cuts at: 3839 4995 3839
Size: 1156 4626
ApoI r'AATT_y
Cuts at: 1493 2879 3091 3317 3469 5578 1493
Size: 1386 212 226 152 2109 1697
Fragments arranged by size:
2109 1697 1386 226 212 152
AseI AT'TA_AT
Cuts at: 160 1032 4336 160
Size: 872 3304 1606
Fragments arranged by size:
3304 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5782
AvaI C'yCGr_G
Cuts at: 1870 1943 3107 1870
Size: 73 1164 4545
Fragments arranged by size:
4545 1164 73
BamHI G'GATC_C
Cuts at: 3112 3479 3112
Size: 367 5415
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2928 3103 4558 618
Size: 325 217 627 1141 175 1455 1842
Fragments arranged by size:
1842 1455 1141 627 325 217 175
BanII G_rGCy'C
Cuts at: 1391 2117 2663 2724 2833 3101 1391
Size: 726 546 61 109 268 4072
Fragments arranged by size:
4072 726 546 268 109 61
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4811
BbvCI CC'TCA_GC
Cuts at: 2050 2050
Size: 5782
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3973 4007 1903
Size: 34 2036 34 3678
Fragments arranged by size:
3678 2036 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3079 3674 4884 3079
Size: 595 1210 3977
Fragments arranged by size:
3977 1210 595
BclI T'GATC_A
Cuts at: 1548 2836 1548
Size: 1288 4494
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4393 136
Size: 107 122 71 631 605 2721 1525
Fragments arranged by size:
2721 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5777 5777
Size: 5782
BlpI GC'TnA_GC
Cuts at: 2111 3199 2111
Size: 1088 4694
Bme1580I G_kGCm'C
Cuts at: 1790 3843 4999 1790
Size: 2053 1156 2573
Fragments arranged by size:
2573 2053 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5782
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2727 3513 4471
Size: 416 53 83 399 24 1310 786 958
Cuts at: 4471 442
Size: 1753
Fragments arranged by size:
1753 1310 958 786 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2448 1056
Size: 1392 4390
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2976 4442 1239
Size: 1737 1466 2579
Fragments arranged by size:
2579 1737 1466
Bpu10I CC'TnA_GC
Cuts at: 2050 2603 2050
Size: 553 5229
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3907 4772 5070 3907
Size: 865 298 4619
Fragments arranged by size:
4619 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4445 882
Size: 617 2946 2219
Fragments arranged by size:
2946 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3079 3573 493
Size: 711 1875 494 2702
Fragments arranged by size:
2702 1875 711 494
BsaBI GATnn'nnATC
Cuts at: 5776 5776
Size: 5782
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3589 3971 4606 275
Size: 53 83 186 936 2056 382 635 1451
Fragments arranged by size:
2056 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2263 4215 4613 4887 5034 5445 2263
Size: 1952 398 274 147 411 2600
Fragments arranged by size:
2600 1952 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2775 2805 1178
Size: 30 165 30 1372 30 4155
Fragments arranged by size:
4155 1372 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2114 1186
Size: 928 4854
BseYI C'CCAG_C
Cuts at: 2314 4985 5481 2314
Size: 2671 496 2615
Fragments arranged by size:
2671 2615 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5209
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3232 3993 5021 1540
Size: 275 186 1231 761 1028 2301
Fragments arranged by size:
2301 1231 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3843 3928 4999 3843
Size: 85 1071 4626
Fragments arranged by size:
4626 1071 85
BsmI GAATG_Cn'
Cuts at: 3293 3386 3293
Size: 93 5689
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2117 2663 2724 2833 3101 3843 3928
Size: 399 327 546 61 109 268 742 85
Cuts at: 3928 4999 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 546 399 327 268 109
85 61
BspEI T'CCGG_A
Cuts at: 5445 5445
Size: 5782
BspHI T'CATG_A
Cuts at: 3671 5401 3671
Size: 1730 4052
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2204 2487 3137 844
Size: 1360 283 650 3489
Fragments arranged by size:
3489 1360 650 283
BsrBI CCG'CTC
Cuts at: 1394 1823 3669 1394
Size: 429 1846 3507
Fragments arranged by size:
3507 1846 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4277 4451 65
Size: 1183 3029 174 1396
Fragments arranged by size:
3029 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4426 4613 1700
Size: 110 2616 187 2869
Fragments arranged by size:
2869 2616 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5782
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5782
BssSI C'ACGA_G
Cuts at: 3842 4854 3842
Size: 1012 4770
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5782
BstBI TT'CG_AA
Cuts at: 2007 4586 2007
Size: 2579 3203
BstEII G'GTnAC_C
Cuts at: 1184 2266 1184
Size: 1082 4700
BstYI r'GATC_y
Cuts at: 1972 3112 3479 3864 3881 5333 5419 5431 5777
Size: 1140 367 385 17 1452 86 12 346
Cuts at: 5777 1972
Size: 1977
Fragments arranged by size:
1977 1452 1140 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2566 513
Size: 552 957 544 3729
Fragments arranged by size:
3729 957 552 544
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5043
Fragments arranged by size:
5043 683 56
BtsI GCAGTG_nn'
Cuts at: 2701 2716 3298 4092 4112 2701
Size: 15 582 794 20 4371
Fragments arranged by size:
4371 794 582 20 15
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5782
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5747
DraI TTT'AAA
Cuts at: 3086 3086
Size: 5782
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4789 809
Size: 507 3473 1802
Fragments arranged by size:
3473 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2912 3229 4118
Size: 54 1425 50 275 18 1082 317 889
Cuts at: 4118 5747 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1082 889 317 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3229 1537
Size: 275 1417 4090
Fragments arranged by size:
4090 1417 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3712 1353
Size: 2359 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2425 4369 4743 4889 387
Size: 1064 138 836 1944 374 146 1280
Fragments arranged by size:
1944 1280 1064 836 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2435 2483 2868 2976 3845 4442 5229
Size: 393 803 48 385 108 869 597 787
Cuts at: 5229 5497 1239
Size: 268 1524
Fragments arranged by size:
1524 869 803 787 597 393 385 268
108 48
EcoNI CCTnn'n_nnAGG
Cuts at: 2978 2978
Size: 5782
EcoRI G'AATT_C
Cuts at: 3091 3469 3091
Size: 378 5404
FspI TGC'GCA
Cuts at: 4288 4288
Size: 5782
HaeII r_GCGC'y
Cuts at: 1678 2111 4929 1678
Size: 433 2818 2531
Fragments arranged by size:
2818 2531 433
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2033 2051 2065 2083 2660
Size: 32 135 32 461 18 14 18 577
Cuts at: 2660 2692 2775 2790 2807 2822 4423 4455 4497
Size: 32 83 15 17 15 1601 32 42
Cuts at: 4497 4529 1373
Size: 32 2626
Fragments arranged by size:
2626 1601 577 461 135 83 42 32
32 32 32 32 18 18 17 15
15 14
HincII GTy'rAC
Cuts at: 669 1657 1938 2393 3126 3372 669
Size: 988 281 455 733 246 3079
Fragments arranged by size:
3079 988 733 455 281 246
HindIII A'AGCT_T
Cuts at: 748 2081 748
Size: 1333 4449
HpaI GTT'AAC
Cuts at: 3372 3372
Size: 5782
KpnI G_GTAC'C
Cuts at: 2932 3107 2932
Size: 175 5607
MfeI C'AATT_G
Cuts at: 3381 3381
Size: 5782
MluI A'CGCG_T
Cuts at: 5658 5658
Size: 5782
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2135 4896 5080 1305
Size: 830 2761 184 2007
Fragments arranged by size:
2761 2007 830 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3960
Fragments arranged by size:
3960 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3740 4099 4258 518
Size: 648 2574 359 159 2042
Fragments arranged by size:
2574 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2532 3198 3875 5023 5268 1606
Size: 926 666 677 1148 245 2120
Fragments arranged by size:
2120 1148 926 677 666 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4273
Fragments arranged by size:
4273 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5782
NheI G'CTAG_C
Cuts at: 1052 2444 1052
Size: 1392 4390
NotI GC'GGCC_GC
Cuts at: 3229 3229
Size: 5782
NspI r_CATG'y
Cuts at: 1132 1229 2677 3140 4685 1132
Size: 97 1448 463 1545 2229
Fragments arranged by size:
2229 1545 1448 463 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5782
PciI A'CATG_T
Cuts at: 2673 4681 2673
Size: 2008 3774
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5782
PmeI GTTT'AAAC
Cuts at: 3086 3086
Size: 5782
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3851 3883 5364 5396 3851
Size: 32 1481 32 4237
Fragments arranged by size:
4237 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5782
PsiI TTA'TAA
Cuts at: 3352 3352
Size: 5782
PstI C_TGCA'G
Cuts at: 830 2498 2965 3134 830
Size: 1668 467 169 3478
Fragments arranged by size:
3478 1668 467 169
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5782
SalI G'TCGA_C
Cuts at: 1936 2391 3124 1936
Size: 455 733 4594
Fragments arranged by size:
4594 733 455
SbfI CC_TGCA'GG
Cuts at: 3134 3134
Size: 5782
ScaI AGT'ACT
Cuts at: 4030 5742 4030
Size: 1712 4070
SfcI C'TryA_G
Cuts at: 826 1045 2203 2494 2961 3130 4265 4946 5137
Size: 219 1158 291 467 169 1135 681 191
Cuts at: 5137 826
Size: 1471
Fragments arranged by size:
1471 1158 1135 681 467 291 219 191
169
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5782
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5782
SmaI CCC'GGG
Cuts at: 1872 3109 1872
Size: 1237 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3886 4787 5049 820
Size: 197 926 1943 901 262 1553
Fragments arranged by size:
1943 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3079 3573 493
Size: 2586 494 2702
Fragments arranged by size:
2702 2586 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5782
SphI G_CATG'C
Cuts at: 1132 3140 1132
Size: 2008 3774
SspI AAT'ATT
Cuts at: 5 52 3049 3706 5
Size: 47 2997 657 2081
Fragments arranged by size:
2997 2081 657 47
StuI AGG'CCT
Cuts at: 1517 2213 1517
Size: 696 5086
StyI C'CwwG_G
Cuts at: 513 1065 2022 2055 3221 513
Size: 552 957 33 1166 3074
Fragments arranged by size:
3074 1166 957 552 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2328 3809 3826 3979 4164 4609 325
Size: 1255 748 1481 17 153 185 445 1498
Fragments arranged by size:
1498 1481 1255 748 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4028 5740 96
Size: 274 80 33 51 1443 2051 1712 138
Fragments arranged by size:
2051 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2620 2652 5742 5774 2620
Size: 32 3090 32 2628
Fragments arranged by size:
3090 2628 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5782
XbaI T'CTAG_A
Cuts at: 3118 3118
Size: 5782
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2029 2029
Size: 5782
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5782
XmaI C'CCGG_G
Cuts at: 1870 3107 1870
Size: 1237 4545
XmnI GAAnn'nnTTC
Cuts at: 2863 3911 2863
Size: 1048 4734
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3590 276
Size: 53 83 186 936 2056 2468
Fragments arranged by size:
2468 2056 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI