Restriction map for
pFN21ASDA1142
(Circular) (Six-base) MAPSORT of: pFN21ASDA1142.seq Check: 9224 from: 1 to: 6043
pFN21ASDA1142 6043 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3853 278
Size: 53 83 186 936 2317 2468
Fragments arranged by size:
2468 2317 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2184 3051 3386 1937
Size: 247 867 335 4594
Fragments arranged by size:
4594 867 335 247
Acc65I G'GTAC_C
Cuts at: 3364 3364
Size: 6043
AclI AA'CG_TT
Cuts at: 1563 4170 4543 1563
Size: 2607 373 3063
Fragments arranged by size:
3063 2607 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3188 3218 3284 4106 5490 5758 1632
Size: 1556 30 66 822 1384 268 1917
Fragments arranged by size:
1917 1556 1384 822 268 66 30
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5846
AflIII A'CryG_T
Cuts at: 1203 4942 5919 1203
Size: 3739 977 1327
Fragments arranged by size:
3739 1327 977
AgeI A'CCGG_T
Cuts at: 4874 4874
Size: 6043
AhdI GACnn_n'nnGTC
Cuts at: 3058 4772 3058
Size: 1714 4329
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6043
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2984 1184
Size: 1800 4243
AlwNI CAG_nnn'CTG
Cuts at: 2146 2845 3786 5358 2146
Size: 699 941 1572 2831
Fragments arranged by size:
2831 1572 941 699
ApaI G_GGCC'C
Cuts at: 2221 2421 2221
Size: 200 5843
ApaLI G'TGCA_C
Cuts at: 2084 4100 5256 2084
Size: 2016 1156 2871
Fragments arranged by size:
2871 2016 1156
ApoI r'AATT_y
Cuts at: 1493 3352 3578 3730 5839 1493
Size: 1859 226 152 2109 1697
Fragments arranged by size:
2109 1859 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4597 160
Size: 872 3565 1606
Fragments arranged by size:
3565 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6043
AvaI C'yCGr_G
Cuts at: 1870 1943 2213 2316 2322 2590 3368 1870
Size: 73 270 103 6 268 778 4545
Fragments arranged by size:
4545 778 270 268 103 73 6
BamHI G'GATC_C
Cuts at: 3373 3740 3373
Size: 367 5676
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2327 2765 3364 4819 618
Size: 325 217 627 540 438 599 1455 1842
Fragments arranged by size:
1842 1455 627 599 540 438 325 217
BanII G_rGCy'C
Cuts at: 1391 2221 2323 2370 2421 2555 2736 3021 3102
Size: 830 102 47 51 134 181 285 81
Cuts at: 3102 3362 1391
Size: 260 4072
Fragments arranged by size:
4072 830 285 260 181 134 102 81
51 47
BbeI G_GCGC'C
Cuts at: 2769 2769
Size: 6043
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5072
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4234 4268 1903
Size: 34 2297 34 3678
Fragments arranged by size:
3678 2297 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2470 3935 5145 2470
Size: 1465 1210 3368
Fragments arranged by size:
3368 1465 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6043
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3242 4654 136
Size: 107 122 71 631 605 1570 1412 1525
Fragments arranged by size:
1570 1525 1412 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2052 6038 2052
Size: 3986 2057
BlpI GC'TnA_GC
Cuts at: 3460 3460
Size: 6043
Bme1580I G_kGCm'C
Cuts at: 1790 2088 2221 2332 2421 2973 3312 4104 5260
Size: 298 133 111 89 552 339 792 1156
Cuts at: 5260 1790
Size: 2573
Fragments arranged by size:
2573 1156 792 552 339 298 133 111
89
BmgBI CAC'GTC
Cuts at: 1318 2242 1318
Size: 924 5119
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2278 3774 4732
Size: 416 53 83 399 24 861 1496 958
Cuts at: 4732 442
Size: 1753
Fragments arranged by size:
1753 1496 958 861 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6043
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2786 3040 2786
Size: 254 5789
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2361 2367 4703 1239
Size: 1122 6 2336 2579
Fragments arranged by size:
2579 2336 1122 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4168 5033 5331 4168
Size: 865 298 4880
Fragments arranged by size:
4880 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3222 4706 882
Size: 617 1723 1484 2219
Fragments arranged by size:
2219 1723 1484 617
BsaAI yAC'GTr
Cuts at: 493 1204 3340 3834 493
Size: 711 2136 494 2702
Fragments arranged by size:
2702 2136 711 494
BsaBI GATnn'nnATC
Cuts at: 6037 6037
Size: 6043
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2766 2868 3850 4232
Size: 53 83 186 936 1233 102 982 382
Cuts at: 4232 4867 275
Size: 635 1451
Fragments arranged by size:
1451 1233 982 936 635 382 186 102
83 53
BsaWI w'CCGG_w
Cuts at: 4476 4874 5148 5295 5706 4476
Size: 398 274 147 411 4813
Fragments arranged by size:
4813 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2501 2531 1178
Size: 30 165 30 1098 30 4690
Fragments arranged by size:
4690 1098 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2168 2281 2284 2287 2290 2819 1186
Size: 982 113 3 3 3 529 4410
Fragments arranged by size:
4410 982 529 113 3 3 3
BseYI C'CCAG_C
Cuts at: 2333 2347 2370 3064 3237 5246 5742 2333
Size: 14 23 694 173 2009 496 2634
Fragments arranged by size:
2634 2009 694 496 173 23 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2374 3161 1163
Size: 573 638 787 4045
Fragments arranged by size:
4045 787 638 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2820 3493 4254 5282 1540
Size: 275 186 819 673 761 1028 2301
Fragments arranged by size:
2301 1028 819 761 673 275 186
BsiHKAI G_wGCw'C
Cuts at: 2079 2088 2736 2856 3021 4104 4189 5260 2079
Size: 9 648 120 165 1083 85 1071 2862
Fragments arranged by size:
2862 1083 1071 648 165 120 85 9
BsmI GAATG_Cn'
Cuts at: 3554 3647 3554
Size: 93 5950
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2079 2088 2221 2323 2332 2370 2421
Size: 399 289 9 133 102 9 38 51
Cuts at: 2421 2555 2736 2856 2973 3021 3102 3312 3362
Size: 134 181 120 117 48 81 210 50
Cuts at: 3362 4104 4189 5260 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 289 210 181 134
133 120 117 102 85 81 51 50
48 38 9 9
BspEI T'CCGG_A
Cuts at: 5706 5706
Size: 6043
BspHI T'CATG_A
Cuts at: 2236 2670 3932 5662 2236
Size: 434 1262 1730 2617
Fragments arranged by size:
2617 1730 1262 434
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2631 3162 3183 3398 844
Size: 1787 531 21 215 3489
Fragments arranged by size:
3489 1787 531 215 21
BsrBI CCG'CTC
Cuts at: 1394 1823 2433 2517 3930 1394
Size: 429 610 84 1413 3507
Fragments arranged by size:
3507 1413 610 429 84
BsrDI GCAATG_nn'
Cuts at: 65 1248 4538 4712 65
Size: 1183 3290 174 1396
Fragments arranged by size:
3290 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2166 4687 4874 1700
Size: 110 356 2521 187 2869
Fragments arranged by size:
2869 2521 356 187 110
BsrGI T'GTAC_A
Cuts at: 96 2706 96
Size: 2610 3433
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6043
BssSI C'ACGA_G
Cuts at: 2105 2687 4103 5115 2105
Size: 582 1416 1012 3033
Fragments arranged by size:
3033 1416 1012 582
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2083 2568 794
Size: 1289 485 4269
Fragments arranged by size:
4269 1289 485
BstBI TT'CG_AA
Cuts at: 2007 4847 2007
Size: 2840 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6043
BstYI r'GATC_y
Cuts at: 1972 2052 3373 3740 4125 4142 5594 5680 5692
Size: 80 1321 367 385 17 1452 86 12
Cuts at: 5692 6038 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1321 385 367 346 86 80
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2556 3246 513
Size: 552 1491 690 3310
Fragments arranged by size:
3310 1491 690 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2557 487
Size: 683 56 1331 3973
Fragments arranged by size:
3973 1331 683 56
BtsI GCAGTG_nn'
Cuts at: 2838 3559 4353 4373 2838
Size: 721 794 20 4508
Fragments arranged by size:
4508 794 721 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6043
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6008
DraI TTT'AAA
Cuts at: 3347 3347
Size: 6043
DraIII CAC_nnn'GTG
Cuts at: 2083 3184 2083
Size: 1101 4942
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5050 809
Size: 507 3734 1802
Fragments arranged by size:
3734 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2274 2604 2817
Size: 54 1425 50 275 18 444 330 213
Cuts at: 2817 3279 3490 4379 6008 8
Size: 462 211 889 1629 43
Fragments arranged by size:
1629 1425 889 462 444 330 275 213
211 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2817 3490 1537
Size: 275 1005 673 4090
Fragments arranged by size:
4090 1005 673 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2027 2657 3973 1353
Size: 674 630 1316 3423
Fragments arranged by size:
3423 1316 674 630
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4630 5004 5150 387
Size: 1064 138 3041 374 146 1280
Fragments arranged by size:
3041 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2734 3019 2734
Size: 285 5758
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2361 2367 3188 3218 3284 4106 4703
Size: 393 729 6 821 30 66 822 597
Cuts at: 4703 5490 5758 1239
Size: 787 268 1524
Fragments arranged by size:
1524 822 821 787 729 597 393 268
66 30 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2150 3179 3281 2150
Size: 1029 102 4912
Fragments arranged by size:
4912 1029 102
EcoO109I rG'GnC_Cy
Cuts at: 2217 2218 2269 2351 2418 2842 2217
Size: 1 51 82 67 424 5418
Fragments arranged by size:
5418 424 82 67 51 1
EcoRI G'AATT_C
Cuts at: 3352 3730 3352
Size: 378 5665
FspI TGC'GCA
Cuts at: 2585 2627 4549 2585
Size: 42 1922 4079
Fragments arranged by size:
4079 1922 42
HaeII r_GCGC'y
Cuts at: 1678 2769 5190 1678
Size: 1091 2421 2531
Fragments arranged by size:
2531 2421 1091
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2501 2533 3044 3076 4684
Size: 32 135 32 929 32 511 32 1608
Cuts at: 4684 4716 4758 4790 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1608 929 511 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2185 3387 3633 669
Size: 988 281 247 1202 246 3079
Fragments arranged by size:
3079 1202 988 281 247 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6043
HpaI GTT'AAC
Cuts at: 3633 3633
Size: 6043
KasI G'GCGC_C
Cuts at: 2765 2765
Size: 6043
KpnI G_GTAC'C
Cuts at: 3368 3368
Size: 6043
MfeI C'AATT_G
Cuts at: 3642 3642
Size: 6043
MluI A'CGCG_T
Cuts at: 5919 5919
Size: 6043
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2091 5157 5341 1305
Size: 786 3066 184 2007
Fragments arranged by size:
3066 2007 786 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2276 3281 10
Size: 54 1425 343 444 1005 2772
Fragments arranged by size:
2772 1425 1005 444 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2691 2795 3137 4001 4360 4519 518
Size: 648 1525 104 342 864 359 159 2042
Fragments arranged by size:
2042 1525 864 648 359 342 159 104
MspA1I CmG'CkG
Cuts at: 1606 2264 2374 2443 2491 3241 3459 4136 5284
Size: 658 110 69 48 750 218 677 1148
Cuts at: 5284 5529 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 750 677 658 245 218 110
69 48
NaeI GCC'GGC
Cuts at: 2168 2168
Size: 6043
NarI GG'CG_CC
Cuts at: 2766 2766
Size: 6043
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5491
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6043
NgoMIV G'CCGG_C
Cuts at: 2166 2166
Size: 6043
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6043
NotI GC'GGCC_GC
Cuts at: 3490 3490
Size: 6043
NspI r_CATG'y
Cuts at: 1132 1229 3401 4946 1132
Size: 97 2172 1545 2229
Fragments arranged by size:
2229 2172 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2863 1348
Size: 1515 4528
PciI A'CATG_T
Cuts at: 4942 4942
Size: 6043
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6043
PfoI T'CCnGG_A
Cuts at: 2754 3207 2754
Size: 453 5590
PmeI GTTT'AAAC
Cuts at: 3347 3347
Size: 6043
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2099 2131 4112 4144 5625 5657 2099
Size: 32 1981 32 1481 32 2485
Fragments arranged by size:
2485 1981 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2351 2351
Size: 6043
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6043
PsiI TTA'TAA
Cuts at: 3613 3613
Size: 6043
PspOMI G'GGCC_C
Cuts at: 2217 2417 2217
Size: 200 5843
PstI C_TGCA'G
Cuts at: 830 2490 2841 3395 830
Size: 1660 351 554 3478
Fragments arranged by size:
3478 1660 554 351
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6043
PvuII CAG'CTG
Cuts at: 2374 2491 2374
Size: 117 5926
SacI G_AGCT'C
Cuts at: 2736 3021 2736
Size: 285 5758
SalI G'TCGA_C
Cuts at: 1936 2183 3385 1936
Size: 247 1202 4594
Fragments arranged by size:
4594 1202 247
SanDI GG'GwC_CC
Cuts at: 2351 2351
Size: 6043
SapI GCTCTTCn'nnn_
Cuts at: 2657 2657
Size: 6043
SbfI CC_TGCA'GG
Cuts at: 3395 3395
Size: 6043
ScaI AGT'ACT
Cuts at: 4291 6003 4291
Size: 1712 4331
SexAI A'CCwGG_T
Cuts at: 2718 2718
Size: 6043
SfcI C'TryA_G
Cuts at: 826 1045 2486 2837 3391 4526 5207 5398 826
Size: 219 1441 351 554 1135 681 191 1471
Fragments arranged by size:
1471 1441 1135 681 554 351 219 191
SfoI GGC'GCC
Cuts at: 2767 2767
Size: 6043
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6043
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6043
SmaI CCC'GGG
Cuts at: 1872 2215 3370 1872
Size: 343 1155 4545
Fragments arranged by size:
4545 1155 343
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4147 5048 5310 820
Size: 197 926 2204 901 262 1553
Fragments arranged by size:
2204 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3340 3834 493
Size: 2847 494 2702
Fragments arranged by size:
2847 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6043
SphI G_CATG'C
Cuts at: 1132 3401 1132
Size: 2269 3774
SspI AAT'ATT
Cuts at: 5 52 3967 5
Size: 47 3915 2081
Fragments arranged by size:
3915 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6043
StyI C'CwwG_G
Cuts at: 513 1065 2223 3282 3482 513
Size: 552 1158 1059 200 3074
Fragments arranged by size:
3074 1158 1059 552 200
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2728 2801 3285 4070 4087 4240 4425
Size: 1255 1148 73 484 785 17 153 185
Cuts at: 4425 4870 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1148 785 484 445 185 153
73 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2706 4289 6001
Size: 274 80 33 51 1443 729 1583 1712
Cuts at: 6001 96
Size: 138
Fragments arranged by size:
1712 1583 1443 729 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6003 6035 6003
Size: 32 6011
Tth111I GACn'n_nGTC
Cuts at: 1316 2780 1316
Size: 1464 4579
XbaI T'CTAG_A
Cuts at: 3379 3379
Size: 6043
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6043
XmaI C'CCGG_G
Cuts at: 1870 2213 3368 1870
Size: 343 1155 4545
Fragments arranged by size:
4545 1155 343
XmnI GAAnn'nnTTC
Cuts at: 3203 4172 3203
Size: 969 5074
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3851 276
Size: 53 83 186 936 2317 2468
Fragments arranged by size:
2468 2317 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FalI
FseI FspAI NruI NsiI PacI PmlI PspXI PsrI
RsrII SacII SfiI SrfI SwaI XcmI