Restriction map for
pFN21ASDA1135
(Circular) (Six-base) MAPSORT of: pFN21ASDA1135.seq Check: 7018 from: 1 to: 6997
pFN21ASDA1135 6997 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3346 3346
Size: 6997
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4807 278
Size: 53 83 186 936 3271 2468
Fragments arranged by size:
3271 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3446 4340 1937
Size: 1509 894 4594
Fragments arranged by size:
4594 1509 894
Acc65I G'GTAC_C
Cuts at: 4318 4318
Size: 6997
AclI AA'CG_TT
Cuts at: 1563 5124 5497 1563
Size: 3561 373 3063
Fragments arranged by size:
3561 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3501 4251 5060 6444 6712 1632
Size: 1869 750 809 1384 268 1917
Fragments arranged by size:
1917 1869 1384 809 750 268
AflII C'TTAA_G
Cuts at: 820 1017 2756 2879 820
Size: 197 1739 123 4938
Fragments arranged by size:
4938 1739 197 123
AflIII A'CryG_T
Cuts at: 1203 4233 5896 6873 1203
Size: 3030 1663 977 1327
Fragments arranged by size:
3030 1663 1327 977
AgeI A'CCGG_T
Cuts at: 5828 5828
Size: 6997
AhdI GACnn_n'nnGTC
Cuts at: 5726 5726
Size: 6997
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6997
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3378 3914 1184
Size: 2194 536 4267
Fragments arranged by size:
4267 2194 536
AlwNI CAG_nnn'CTG
Cuts at: 3628 3730 4740 6312 3628
Size: 102 1010 1572 4313
Fragments arranged by size:
4313 1572 1010 102
ApaI G_GGCC'C
Cuts at: 4204 4204
Size: 6997
ApaLI G'TGCA_C
Cuts at: 2688 5054 6210 2688
Size: 2366 1156 3475
Fragments arranged by size:
3475 2366 1156
ApoI r'AATT_y
Cuts at: 1493 3527 3665 4306 4532 4684 6793 1493
Size: 2034 138 641 226 152 2109 1697
Fragments arranged by size:
2109 2034 1697 641 226 152 138
AseI AT'TA_AT
Cuts at: 160 1032 5551 160
Size: 872 4519 1606
Fragments arranged by size:
4519 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6997
AvaI C'yCGr_G
Cuts at: 1870 1943 2099 2130 4322 1870
Size: 73 156 31 2192 4545
Fragments arranged by size:
4545 2192 156 73 31
BamHI G'GATC_C
Cuts at: 4327 4694 4327
Size: 367 6630
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2303 2317 3358 4318 5773
Size: 325 217 627 516 14 1041 960 1455
Cuts at: 5773 618
Size: 1842
Fragments arranged by size:
1842 1455 1041 960 627 516 325 217
14
BanII G_rGCy'C
Cuts at: 1391 2100 2129 2715 3391 4204 4316 1391
Size: 709 29 586 676 813 112 4072
Fragments arranged by size:
4072 813 709 676 586 112 29
BbeI G_GCGC'C
Cuts at: 2307 2321 2307
Size: 14 6983
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6026
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5188 5222 1903
Size: 34 3251 34 3678
Fragments arranged by size:
3678 3251 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4889 6099 4889
Size: 1210 5787
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6997
BfrBI ATG'CAT
Cuts at: 3715 3715
Size: 6997
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2046 3640 5608
Size: 107 122 71 631 605 374 1594 1968
Cuts at: 5608 136
Size: 1525
Fragments arranged by size:
1968 1594 1525 631 605 374 122 107
71
BglII A'GATC_T
Cuts at: 4287 6992 4287
Size: 2705 4292
BlpI GC'TnA_GC
Cuts at: 2141 4414 2141
Size: 2273 4724
Bme1580I G_kGCm'C
Cuts at: 1790 2692 2833 4204 5058 6214 1790
Size: 902 141 1371 854 1156 2573
Fragments arranged by size:
2573 1371 1156 902 854 141
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6997
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2345 2836 3671
Size: 416 53 83 399 24 928 491 835
Cuts at: 3671 4728 5686 442
Size: 1057 958 1753
Fragments arranged by size:
1753 1057 958 928 835 491 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6997
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2168 2314 3515 4064 4129 5657 1239
Size: 929 146 1201 549 65 1528 2579
Fragments arranged by size:
2579 1528 1201 929 549 146 65
Bpu10I CC'TnA_GC
Cuts at: 2028 2028
Size: 6997
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3680 5122 5987 6285 3680
Size: 1442 865 298 4392
Fragments arranged by size:
4392 1442 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5660 882
Size: 617 4161 2219
Fragments arranged by size:
4161 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4294 4788 493
Size: 711 3090 494 2702
Fragments arranged by size:
3090 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 6991 6991
Size: 6997
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2304 2318 4804 5186
Size: 53 83 186 936 771 14 2486 382
Cuts at: 5186 5821 275
Size: 635 1451
Fragments arranged by size:
2486 1451 936 771 635 382 186 83
53 14
BsaWI w'CCGG_w
Cuts at: 5430 5828 6102 6249 6660 5430
Size: 398 274 147 411 5767
Fragments arranged by size:
5767 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2180 2210 3111 3141 3488
Size: 30 165 30 777 30 901 30 347
Cuts at: 3488 3518 1178
Size: 30 4657
Fragments arranged by size:
4657 901 777 347 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2165 2201 3203 3371 4030 1186
Size: 979 36 1002 168 659 4153
Fragments arranged by size:
4153 1002 979 659 168 36
BseYI C'CCAG_C
Cuts at: 3552 6200 6696 3552
Size: 2648 496 3853
Fragments arranged by size:
3853 2648 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3348 3422 1163
Size: 573 1612 74 4738
Fragments arranged by size:
4738 1612 573 74
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2121 2277 2328 4447 5208 6236
Size: 275 186 120 156 51 2119 761 1028
Cuts at: 6236 1540
Size: 2301
Fragments arranged by size:
2301 2119 1028 761 275 186 156 120
51
BsiHKAI G_wGCw'C
Cuts at: 2692 2715 3034 3881 4115 5058 5143 6214 2692
Size: 23 319 847 234 943 85 1071 3475
Fragments arranged by size:
3475 1071 943 847 319 234 85 23
BsmI GAATG_Cn'
Cuts at: 3689 4508 4601 3689
Size: 819 93 6085
Fragments arranged by size:
6085 819 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3556 3574 3556
Size: 18 6979
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2100 2129 2692 2715 2833 3034 3391
Size: 399 310 29 563 23 118 201 357
Cuts at: 3391 3881 4115 4204 4316 5058 5143 6214 1391
Size: 490 234 89 112 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 563 490 399 357 310
234 201 118 112 89 85 29 23
BspEI T'CCGG_A
Cuts at: 6660 6660
Size: 6997
BspHI T'CATG_A
Cuts at: 2619 3034 4886 6616 2619
Size: 415 1852 1730 3000
Fragments arranged by size:
3000 1852 1730 415
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3346 4352 844
Size: 2502 1006 3489
Fragments arranged by size:
3489 2502 1006
BsrBI CCG'CTC
Cuts at: 1394 1823 2896 3654 4884 1394
Size: 429 1073 758 1230 3507
Fragments arranged by size:
3507 1230 1073 758 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2536 3219 4026 5492 5666 65
Size: 1183 1288 683 807 1466 174 1396
Fragments arranged by size:
1466 1396 1288 1183 807 683 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2108 3871 4170 5641 5828 1700
Size: 110 298 1763 299 1471 187 2869
Fragments arranged by size:
2869 1763 1471 299 298 187 110
BsrGI T'GTAC_A
Cuts at: 96 2166 96
Size: 2070 4927
BssHII G'CGCG_C
Cuts at: 1925 2230 1925
Size: 305 6692
BssSI C'ACGA_G
Cuts at: 5057 6069 5057
Size: 1012 5985
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2696 3628 794
Size: 1902 932 4163
Fragments arranged by size:
4163 1902 932
BstBI TT'CG_AA
Cuts at: 2007 5801 2007
Size: 3794 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6997
BstXI CCAn_nnnn'nTGG
Cuts at: 2512 2512
Size: 6997
BstYI r'GATC_y
Cuts at: 1972 2154 2283 3059 4287 4327 4694 5079 5096
Size: 182 129 776 1228 40 367 385 17
Cuts at: 5096 6548 6634 6646 6992 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1228 776 385 367 346 182
129 86 40 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2060 2090 2322 3113 3119 4116
Size: 552 957 38 30 232 791 6 997
Cuts at: 4116 513
Size: 3394
Fragments arranged by size:
3394 997 957 791 552 232 38 30
6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2799 487
Size: 683 56 1573 4685
Fragments arranged by size:
4685 1573 683 56
BtsI GCAGTG_nn'
Cuts at: 2079 3999 4513 5307 5327 2079
Size: 1920 514 794 20 3749
Fragments arranged by size:
3749 1920 794 514 20
ClaI AT'CG_AT
Cuts at: 1554 2493 1554
Size: 939 6058
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6962
DraI TTT'AAA
Cuts at: 3075 4301 3075
Size: 1226 5771
DraIII CAC_nnn'GTG
Cuts at: 2266 2266
Size: 6997
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2306 6004 809
Size: 507 990 3698 1802
Fragments arranged by size:
3698 1802 990 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2118 2274 2325
Size: 54 1425 50 275 18 288 156 51
Cuts at: 2325 2502 3116 3218 3399 4013 4188 4444 5333
Size: 177 614 102 181 614 175 256 889
Cuts at: 5333 6962 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 614 614 288 275 256
181 177 175 156 102 54 51 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2118 2274 2325 4444 1537
Size: 275 306 156 51 2119 4090
Fragments arranged by size:
4090 2119 306 275 156 51
EarI CTCTTCn'nnn_
Cuts at: 1353 2802 3477 3933 4927 1353
Size: 1449 675 456 994 3423
Fragments arranged by size:
3423 1449 994 675 456
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5584 5958 6104 387
Size: 1064 138 3995 374 146 1280
Fragments arranged by size:
3995 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2713 2713
Size: 6997
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2168 2314 3501 3515 4064 4129 4251
Size: 393 536 146 1187 14 549 65 122
Cuts at: 4251 5060 5657 6444 6712 1239
Size: 809 597 787 268 1524
Fragments arranged by size:
1524 1187 809 787 597 549 536 393
268 146 122 65 14
EcoNI CCTnn'n_nnAGG
Cuts at: 3734 3734
Size: 6997
EcoO109I rG'GnC_Cy
Cuts at: 3727 3947 4200 4247 3727
Size: 220 253 47 6477
Fragments arranged by size:
6477 253 220 47
EcoRI G'AATT_C
Cuts at: 3665 4306 4684 3665
Size: 641 378 5978
Fragments arranged by size:
5978 641 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2771 3820 2771
Size: 1049 5948
FspI TGC'GCA
Cuts at: 5503 5503
Size: 6997
HaeII r_GCGC'y
Cuts at: 1678 2307 2321 6144 1678
Size: 629 14 3823 2531
Fragments arranged by size:
3823 2531 629 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3488 3520 5638 5670 5712
Size: 32 135 32 1916 32 2118 32 42
Cuts at: 5712 5744 1373
Size: 32 2626
Fragments arranged by size:
2626 2118 1916 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3447 4341 4587 669
Size: 988 281 1509 894 246 3079
Fragments arranged by size:
3079 1509 988 894 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6997
HpaI GTT'AAC
Cuts at: 4587 4587
Size: 6997
KasI G'GCGC_C
Cuts at: 2303 2317 2303
Size: 14 6983
KpnI G_GTAC'C
Cuts at: 4322 4322
Size: 6997
MfeI C'AATT_G
Cuts at: 4596 4596
Size: 6997
MluI A'CGCG_T
Cuts at: 6873 6873
Size: 6997
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6111 6295 1305
Size: 4806 184 2007
Fragments arranged by size:
4806 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2504 4190 10
Size: 54 1425 343 672 1686 2817
Fragments arranged by size:
2817 1686 1425 672 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4121 4955 5314 5473 518
Size: 648 2955 834 359 159 2042
Fragments arranged by size:
2955 2042 834 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2062 2092 2324 3370 4413 5090 6238 6483
Size: 456 30 232 1046 1043 677 1148 245
Cuts at: 6483 1606
Size: 2120
Fragments arranged by size:
2120 1148 1046 1043 677 456 245 232
30
NaeI GCC'GGC
Cuts at: 2110 4172 2110
Size: 2062 4935
NarI GG'CG_CC
Cuts at: 2304 2318 2304
Size: 14 6983
NcoI C'CATG_G
Cuts at: 513 1065 2022 4116 513
Size: 552 957 2094 3394
Fragments arranged by size:
3394 2094 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6997
NgoMIV G'CCGG_C
Cuts at: 2108 4170 2108
Size: 2062 4935
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6997
NotI GC'GGCC_GC
Cuts at: 4444 4444
Size: 6997
NsiI A_TGCA'T
Cuts at: 3717 3717
Size: 6997
NspI r_CATG'y
Cuts at: 1132 1229 3232 4237 4355 5900 1132
Size: 97 2003 1005 118 1545 2229
Fragments arranged by size:
2229 2003 1545 1005 118 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6997
PciI A'CATG_T
Cuts at: 4233 5896 4233
Size: 1663 5334
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3730 1108
Size: 2622 4375
PfoI T'CCnGG_A
Cuts at: 3492 3492
Size: 6997
PmeI GTTT'AAAC
Cuts at: 4301 4301
Size: 6997
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2011 2043 5066 5098 6579 6611 2011
Size: 32 3023 32 1481 32 2397
Fragments arranged by size:
3023 2397 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3727 3947 3727
Size: 220 6777
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6997
PsiI TTA'TAA
Cuts at: 2424 4567 2424
Size: 2143 4854
PspOMI G'GGCC_C
Cuts at: 4200 4200
Size: 6997
PstI C_TGCA'G
Cuts at: 830 2554 4349 830
Size: 1724 1795 3478
Fragments arranged by size:
3478 1795 1724
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6997
SacI G_AGCT'C
Cuts at: 2715 2715
Size: 6997
SacII CC_GC'GG
Cuts at: 2063 2093 2325 2063
Size: 30 232 6735
Fragments arranged by size:
6735 232 30
SalI G'TCGA_C
Cuts at: 1936 3445 4339 1936
Size: 1509 894 4594
Fragments arranged by size:
4594 1509 894
SapI GCTCTTCn'nnn_
Cuts at: 2802 3477 2802
Size: 675 6322
SbfI CC_TGCA'GG
Cuts at: 4349 4349
Size: 6997
ScaI AGT'ACT
Cuts at: 4259 5245 6957 4259
Size: 986 1712 4299
Fragments arranged by size:
4299 1712 986
SfcI C'TryA_G
Cuts at: 826 1045 2550 4345 5480 6161 6352 826
Size: 219 1505 1795 1135 681 191 1471
Fragments arranged by size:
1795 1505 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2305 2319 2305
Size: 14 6983
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6997
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6997
SmaI CCC'GGG
Cuts at: 1872 2101 2132 4324 1872
Size: 229 31 2192 4545
Fragments arranged by size:
4545 2192 229 31
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2756 2879 3659 5101 6002 6264
Size: 197 926 813 123 780 1442 901 262
Cuts at: 6264 820
Size: 1553
Fragments arranged by size:
1553 1442 926 901 813 780 262 197
123
SnaBI TAC'GTA
Cuts at: 493 4294 4788 493
Size: 3801 494 2702
Fragments arranged by size:
3801 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6997
SphI G_CATG'C
Cuts at: 1132 4355 1132
Size: 3223 3774
SspI AAT'ATT
Cuts at: 5 52 3141 4921 5
Size: 47 3089 1780 2081
Fragments arranged by size:
3089 2081 1780 47
StuI AGG'CCT
Cuts at: 1517 2562 1517
Size: 1045 5952
StyI C'CwwG_G
Cuts at: 513 1065 2022 4116 4436 513
Size: 552 957 2094 320 3074
Fragments arranged by size:
3074 2094 957 552 320
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2592 5024 5041 5194 5379 5824 325
Size: 1255 1012 2432 17 153 185 445 1498
Fragments arranged by size:
2432 1498 1255 1012 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2166 2901 4257
Size: 274 80 33 51 1443 189 735 1356
Cuts at: 4257 5243 6955 96
Size: 986 1712 138
Fragments arranged by size:
1712 1443 1356 986 735 274 189 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2180 2212 3111 3143 4267 4299 6957 6989 2180
Size: 32 899 32 1124 32 2658 32 2188
Fragments arranged by size:
2658 2188 1124 899 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6997
XbaI T'CTAG_A
Cuts at: 4333 4333
Size: 6997
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4096 4096
Size: 6997
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6997
XmaI C'CCGG_G
Cuts at: 1870 2099 2130 4322 1870
Size: 229 31 2192 4545
Fragments arranged by size:
4545 2192 229 31
XmnI GAAnn'nnTTC
Cuts at: 5126 5126
Size: 6997
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4805 276
Size: 53 83 186 936 3271 2468
Fragments arranged by size:
3271 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BplI BsiWI
BstZ17I Bsu36I EcoRV FseI FspAI NruI PacI PmlI
PspXI PsrI PvuII RsrII SanDI SexAI SfiI SrfI
SwaI