Restriction map for
pFN21ASDA1130
(Circular) (Six-base) MAPSORT of: pFN21ASDA1130.seq Check: 7178 from: 1 to: 6355
pFN21ASDA1130 6355 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3550 3550
Size: 6355
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4165 278
Size: 53 83 186 936 2629 2468
Fragments arranged by size:
2629 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2536 3279 3698 1937
Size: 599 743 419 4594
Fragments arranged by size:
4594 743 599 419
Acc65I G'GTAC_C
Cuts at: 3264 3676 3264
Size: 412 5943
AclI AA'CG_TT
Cuts at: 1563 4482 4855 1563
Size: 2919 373 3063
Fragments arranged by size:
3063 2919 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2279 2497 2983 3046 4418 5802 6070 1632
Size: 647 218 486 63 1372 1384 268 1917
Fragments arranged by size:
1917 1384 1372 647 486 268 218 63
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6158
AflIII A'CryG_T
Cuts at: 1203 5254 6231 1203
Size: 4051 977 1327
Fragments arranged by size:
4051 1327 977
AgeI A'CCGG_T
Cuts at: 5186 5186
Size: 6355
AhdI GACnn_n'nnGTC
Cuts at: 5084 5084
Size: 6355
AleI CACnn'nnGTG
Cuts at: 1166 2723 1166
Size: 1557 4798
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6355
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3236 3268 3236
Size: 32 6323
AlwNI CAG_nnn'CTG
Cuts at: 2783 4098 5670 2783
Size: 1315 1572 3468
Fragments arranged by size:
3468 1572 1315
ApaLI G'TGCA_C
Cuts at: 4412 5568 4412
Size: 1156 5199
ApoI r'AATT_y
Cuts at: 1493 2983 3664 3890 4042 6151 1493
Size: 1490 681 226 152 2109 1697
Fragments arranged by size:
2109 1697 1490 681 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4909 160
Size: 872 3877 1606
Fragments arranged by size:
3877 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6355
AvaI C'yCGr_G
Cuts at: 1870 1943 2604 3599 3680 1870
Size: 73 661 995 81 4545
Fragments arranged by size:
4545 995 661 81 73
BamHI G'GATC_C
Cuts at: 2070 2550 3515 3685 4052 2070
Size: 480 965 170 367 4373
Fragments arranged by size:
4373 965 480 367 170
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3072 3264 3676 5131 618
Size: 325 217 627 1285 192 412 1455 1842
Fragments arranged by size:
1842 1455 1285 627 412 325 217 192
BanII G_rGCy'C
Cuts at: 1391 2113 2324 2994 3291 3643 3674 1391
Size: 722 211 670 297 352 31 4072
Fragments arranged by size:
4072 722 670 352 297 211 31
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2535 3052 928
Size: 971 636 517 4231
Fragments arranged by size:
4231 971 636 517
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4546 4580 1903
Size: 34 2609 34 3678
Fragments arranged by size:
3678 2609 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4247 5457 4247
Size: 1210 5145
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6355
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3051 3604 4966
Size: 107 122 71 631 605 1379 553 1362
Cuts at: 4966 136
Size: 1525
Fragments arranged by size:
1525 1379 1362 631 605 553 122 107
71
BglII A'GATC_T
Cuts at: 6350 6350
Size: 6355
BlpI GC'TnA_GC
Cuts at: 2318 3772 2318
Size: 1454 4901
Bme1580I G_kGCm'C
Cuts at: 1790 2081 4416 5572 1790
Size: 291 2335 1156 2573
Fragments arranged by size:
2573 2335 1156 291
BmgBI CAC'GTC
Cuts at: 1318 2623 1318
Size: 1305 5050
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3515 4086 5044
Size: 416 53 83 399 24 2098 571 958
Cuts at: 5044 442
Size: 1753
Fragments arranged by size:
2098 1753 958 571 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6355
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2286 2324 2830 2922 3047 3290 5015 1239
Size: 1047 38 506 92 125 243 1725 2579
Fragments arranged by size:
2579 1725 1047 506 243 125 92 38
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2864 4480 5345 5643 2864
Size: 1616 865 298 3576
Fragments arranged by size:
3576 1616 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5018 882
Size: 617 3519 2219
Fragments arranged by size:
3519 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3652 4146 493
Size: 711 2448 494 2702
Fragments arranged by size:
2702 2448 711 494
BsaBI GATnn'nnATC
Cuts at: 2966 6349 2966
Size: 3383 2972
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2888 4162 4544 5179
Size: 53 83 186 936 1355 1274 382 635
Cuts at: 5179 275
Size: 1451
Fragments arranged by size:
1451 1355 1274 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2524 2598 4788 5186 5460 5607 6018 2524
Size: 74 2190 398 274 147 411 2861
Fragments arranged by size:
2861 2190 411 398 274 147 74
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2194 2224 1178
Size: 30 165 30 791 30 5309
Fragments arranged by size:
5309 791 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2368 1186
Size: 1182 5173
BseYI C'CCAG_C
Cuts at: 3591 5558 6054 3591
Size: 1967 496 3892
Fragments arranged by size:
3892 1967 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5782
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2134 3805 4566 5594 1540
Size: 275 186 133 1671 761 1028 2301
Fragments arranged by size:
2301 1671 1028 761 275 186 133
BsiHKAI G_wGCw'C
Cuts at: 2994 3291 4416 4501 5572 2994
Size: 297 1125 85 1071 3777
Fragments arranged by size:
3777 1125 1071 297 85
BsmI GAATG_Cn'
Cuts at: 3866 3959 3866
Size: 93 6262
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2081 2113 2324 2994 3291 3643 3674
Size: 399 291 32 211 670 297 352 31
Cuts at: 3674 4416 4501 5572 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 670 399 352 297 291
211 85 32 31
BspEI T'CCGG_A
Cuts at: 2524 6018 2524
Size: 3494 2861
BspHI T'CATG_A
Cuts at: 4244 5974 4244
Size: 1730 4625
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2394 3550 3710 844
Size: 1550 1156 160 3489
Fragments arranged by size:
3489 1550 1156 160
BsrBI CCG'CTC
Cuts at: 1394 1823 2194 4242 1394
Size: 429 371 2048 3507
Fragments arranged by size:
3507 2048 429 371
BsrDI GCAATG_nn'
Cuts at: 65 1248 2581 4850 5024 65
Size: 1183 1333 2269 174 1396
Fragments arranged by size:
2269 1396 1333 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2328 4999 5186 1700
Size: 110 518 2671 187 2869
Fragments arranged by size:
2869 2671 518 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6355
BssHII G'CGCG_C
Cuts at: 1925 2139 1925
Size: 214 6141
BssSI C'ACGA_G
Cuts at: 4415 5427 4415
Size: 1012 5343
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2783 794
Size: 1989 4366
BstBI TT'CG_AA
Cuts at: 2007 5159 2007
Size: 3152 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6355
BstYI r'GATC_y
Cuts at: 1972 2031 2070 2521 2550 2835 3396 3515 3685
Size: 59 39 451 29 285 561 119 170
Cuts at: 3685 4052 4437 4454 5906 5992 6004 6350 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 561 451 385 367 346 285
170 119 86 59 39 29 17 12
Bsu36I CC'TnA_GG
Cuts at: 3090 3614 3090
Size: 524 5831
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5803
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2032 487
Size: 683 56 806 4810
Fragments arranged by size:
4810 806 683 56
BtsI GCAGTG_nn'
Cuts at: 2659 3129 3214 3871 4665 4685 2659
Size: 470 85 657 794 20 4329
Fragments arranged by size:
4329 794 657 470 85 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6355
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6320
DraI TTT'AAA
Cuts at: 3659 3659
Size: 6355
DraIII CAC_nnn'GTG
Cuts at: 2679 2679
Size: 6355
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5362 809
Size: 507 4046 1802
Fragments arranged by size:
4046 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2131 2247 3186
Size: 54 1425 50 275 18 301 116 939
Cuts at: 3186 3802 4691 6320 8
Size: 616 889 1629 43
Fragments arranged by size:
1629 1425 939 889 616 301 275 116
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2131 3802 1537
Size: 275 319 1671 4090
Fragments arranged by size:
4090 1671 319 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2447 4285 1353
Size: 1094 1838 3423
Fragments arranged by size:
3423 1838 1094
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2633 4942 5316 5462 387
Size: 1064 138 1044 2309 374 146 1280
Fragments arranged by size:
2309 1280 1064 1044 374 146 138
EcoICRI GAG'CTC
Cuts at: 2992 3289 2992
Size: 297 6058
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2279 2286 2324 2497 2830 2922 2983
Size: 393 647 7 38 173 333 92 61
Cuts at: 2983 3046 3047 3290 4418 5015 5802 6070 1239
Size: 63 1 243 1128 597 787 268 1524
Fragments arranged by size:
1524 1128 787 647 597 393 333 268
243 173 92 63 61 38 7 1
EcoNI CCTnn'n_nnAGG
Cuts at: 3112 3549 3112
Size: 437 5918
EcoO109I rG'GnC_Cy
Cuts at: 2272 2272
Size: 6355
EcoRI G'AATT_C
Cuts at: 3664 4042 3664
Size: 378 5977
FspI TGC'GCA
Cuts at: 2444 2573 4861 2444
Size: 129 2288 3938
Fragments arranged by size:
3938 2288 129
HaeII r_GCGC'y
Cuts at: 1678 5502 1678
Size: 3824 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2194 2226 2501 2533 3507
Size: 32 135 32 622 32 275 32 974
Cuts at: 3507 3539 4996 5028 5070 5102 1373
Size: 32 1457 32 42 32 2626
Fragments arranged by size:
2626 1457 974 622 275 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3699 3945 669
Size: 988 281 1761 246 3079
Fragments arranged by size:
3079 1761 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6355
HpaI GTT'AAC
Cuts at: 3945 3945
Size: 6355
KpnI G_GTAC'C
Cuts at: 3268 3680 3268
Size: 412 5943
MfeI C'AATT_G
Cuts at: 3954 3954
Size: 6355
MluI A'CGCG_T
Cuts at: 6231 6231
Size: 6355
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2142 5469 5653 1305
Size: 837 3327 184 2007
Fragments arranged by size:
3327 2007 837 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4533
Fragments arranged by size:
4533 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2501 2723 3500 4313 4672 4831 518
Size: 648 1335 222 777 813 359 159 2042
Fragments arranged by size:
2042 1335 813 777 648 359 222 159
MspA1I CmG'CkG
Cuts at: 1606 2257 2302 2358 2701 3261 3371 3771 4448
Size: 651 45 56 343 560 110 400 677
Cuts at: 4448 5596 5841 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 651 560 400 343 245
110 56 45
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5803
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6355
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6355
NotI GC'GGCC_GC
Cuts at: 3802 3802
Size: 6355
NspI r_CATG'y
Cuts at: 1132 1229 3713 5258 1132
Size: 97 2484 1545 2229
Fragments arranged by size:
2484 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6355
PciI A'CATG_T
Cuts at: 5254 5254
Size: 6355
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6355
PfoI T'CCnGG_A
Cuts at: 2337 2487 2337
Size: 150 6205
PmeI GTTT'AAAC
Cuts at: 3659 3659
Size: 6355
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4424 4456 5937 5969 4424
Size: 32 1481 32 4810
Fragments arranged by size:
4810 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2272 2272
Size: 6355
PshAI GACnn'nnGTC
Cuts at: 1613 2891 1613
Size: 1278 5077
PsiI TTA'TAA
Cuts at: 3925 3925
Size: 6355
PstI C_TGCA'G
Cuts at: 830 2632 2700 3044 3136 3404 3534 3707 830
Size: 1802 68 344 92 268 130 173 3478
Fragments arranged by size:
3478 1802 344 268 173 130 92 68
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6355
PvuII CAG'CTG
Cuts at: 2257 2302 2358 3371 2257
Size: 45 56 1013 5241
Fragments arranged by size:
5241 1013 56 45
SacI G_AGCT'C
Cuts at: 2994 3291 2994
Size: 297 6058
SalI G'TCGA_C
Cuts at: 1936 3697 1936
Size: 1761 4594
SbfI CC_TGCA'GG
Cuts at: 3707 3707
Size: 6355
ScaI AGT'ACT
Cuts at: 4603 6315 4603
Size: 1712 4643
SfcI C'TryA_G
Cuts at: 826 1045 2278 2628 2696 3040 3132 3400 3530
Size: 219 1233 350 68 344 92 268 130
Cuts at: 3530 3703 4838 5519 5710 826
Size: 173 1135 681 191 1471
Fragments arranged by size:
1471 1233 1135 681 350 344 268 219
191 173 130 92 68
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6355
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6355
SmaI CCC'GGG
Cuts at: 1872 3682 1872
Size: 1810 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2843 4459 5360 5622 820
Size: 197 926 900 1616 901 262 1553
Fragments arranged by size:
1616 1553 926 901 900 262 197
SnaBI TAC'GTA
Cuts at: 493 3652 4146 493
Size: 3159 494 2702
Fragments arranged by size:
3159 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6355
SphI G_CATG'C
Cuts at: 1132 3713 1132
Size: 2581 3774
SspI AAT'ATT
Cuts at: 5 52 4279 5
Size: 47 4227 2081
Fragments arranged by size:
4227 2081 47
StuI AGG'CCT
Cuts at: 1517 2237 3200 1517
Size: 720 963 4672
Fragments arranged by size:
4672 963 720
StyI C'CwwG_G
Cuts at: 513 1065 2556 3628 3794 513
Size: 552 1491 1072 166 3074
Fragments arranged by size:
3074 1491 1072 552 166
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4382 4399 4552 4737 5182 325
Size: 1255 2802 17 153 185 445 1498
Fragments arranged by size:
2802 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4601 6313 96
Size: 274 80 33 51 1443 2624 1712 138
Fragments arranged by size:
2624 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2105 2137 2898 2930 3001 3033 6315 6347 2105
Size: 32 761 32 71 32 3282 32 2113
Fragments arranged by size:
3282 2113 761 71 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6355
XbaI T'CTAG_A
Cuts at: 3691 3691
Size: 6355
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3368 3368
Size: 6355
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6355
XmaI C'CCGG_G
Cuts at: 1870 3680 1870
Size: 1810 4545
XmnI GAAnn'nnTTC
Cuts at: 3200 4484 3200
Size: 1284 5071
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4163 276
Size: 53 83 186 936 2629 2468
Fragments arranged by size:
2629 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI