Restriction map for
pFN21ASDA1112
(Circular) (Six-base) MAPSORT of: pFN21ASDA1112.seq Check: 908 from: 1 to: 6739
pFN21ASDA1112 6739 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2033 2937 3783 3832 2033
Size: 904 846 49 4940
Fragments arranged by size:
4940 904 846 49
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2682 4549 278
Size: 53 83 186 936 1146 1867 2468
Fragments arranged by size:
2468 1867 1146 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4082 1937
Size: 2145 4594
Acc65I G'GTAC_C
Cuts at: 4060 4060
Size: 6739
AclI AA'CG_TT
Cuts at: 1563 4866 5239 1563
Size: 3303 373 3063
Fragments arranged by size:
3303 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2640 4802 6186 6454 1632
Size: 1008 2162 1384 268 1917
Fragments arranged by size:
2162 1917 1384 1008 268
AfeI AGC'GCT
Cuts at: 3809 3809
Size: 6739
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6542
AflIII A'CryG_T
Cuts at: 1203 2979 3216 3639 5638 6615 1203
Size: 1776 237 423 1999 977 1327
Fragments arranged by size:
1999 1776 1327 977 423 237
AgeI A'CCGG_T
Cuts at: 5570 5570
Size: 6739
AhdI GACnn_n'nnGTC
Cuts at: 5468 5468
Size: 6739
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6739
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2468 1184
Size: 1284 5455
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3056 3088 3056
Size: 32 6707
AlwNI CAG_nnn'CTG
Cuts at: 2159 2447 2659 4482 6054 2159
Size: 288 212 1823 1572 2844
Fragments arranged by size:
2844 1823 1572 288 212
ApaI G_GGCC'C
Cuts at: 2137 2137
Size: 6739
ApaLI G'TGCA_C
Cuts at: 2303 3781 4796 5952 2303
Size: 1478 1015 1156 3090
Fragments arranged by size:
3090 1478 1156 1015
ApoI r'AATT_y
Cuts at: 1493 4048 4274 4426 6535 1493
Size: 2555 226 152 2109 1697
Fragments arranged by size:
2555 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5293 160
Size: 872 4261 1606
Fragments arranged by size:
4261 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6739
AvaI C'yCGr_G
Cuts at: 1870 1943 3529 3543 3794 4064 1870
Size: 73 1586 14 251 270 4545
Fragments arranged by size:
4545 1586 270 251 73 14
AvrII C'CTAG_G
Cuts at: 2186 2186
Size: 6739
BamHI G'GATC_C
Cuts at: 4069 4436 4069
Size: 367 6372
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3107 3755 4060 5515 618
Size: 325 217 627 1320 648 305 1455 1842
Fragments arranged by size:
1842 1455 1320 648 627 325 305 217
BanII G_rGCy'C
Cuts at: 1391 2137 2467 2592 2793 3189 3473 3515 4058
Size: 746 330 125 201 396 284 42 543
Cuts at: 4058 1391
Size: 4072
Fragments arranged by size:
4072 746 543 396 330 284 201 125
42
BbeI G_GCGC'C
Cuts at: 3759 3759
Size: 6739
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2246 928
Size: 971 347 5421
Fragments arranged by size:
5421 971 347
BbvCI CC'TCA_GC
Cuts at: 2771 2900 2771
Size: 129 6610
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3089 3123 3437 3471 4930 4964 1903
Size: 34 1152 34 314 34 1459 34 3678
Fragments arranged by size:
3678 1459 1152 314 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4631 5841 4631
Size: 1210 5529
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6739
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2813 5350 136
Size: 107 122 71 631 605 1141 2537 1525
Fragments arranged by size:
2537 1525 1141 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3141 3300 3496 6734 3141
Size: 159 196 3238 3146
Fragments arranged by size:
3238 3146 196 159
BlpI GC'TnA_GC
Cuts at: 3206 4156 3206
Size: 950 5789
Bme1580I G_kGCm'C
Cuts at: 1790 2137 2307 3785 4800 5956 1790
Size: 347 170 1478 1015 1156 2573
Fragments arranged by size:
2573 1478 1156 1015 347 170
BmgBI CAC'GTC
Cuts at: 1318 2275 3640 1318
Size: 957 1365 4417
Fragments arranged by size:
4417 1365 957
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2049 3493 4470
Size: 416 53 83 399 24 632 1444 977
Cuts at: 4470 5428 442
Size: 958 1753
Fragments arranged by size:
1753 1444 977 958 632 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6739
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3515 3515
Size: 6739
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2255 2334 2347 2518 2577 3083 3140 3698
Size: 1016 79 13 171 59 506 57 558
Cuts at: 3698 3713 5399 1239
Size: 15 1686 2579
Fragments arranged by size:
2579 1686 1016 558 506 171 79 59
57 15 13
Bpu10I CC'TnA_GC
Cuts at: 2771 2900 3095 2771
Size: 129 195 6415
Fragments arranged by size:
6415 195 129
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3458 3543 3800 4864 5729 6027 3458
Size: 85 257 1064 865 298 4170
Fragments arranged by size:
4170 1064 865 298 257 85
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3705 5402 882
Size: 617 2206 1697 2219
Fragments arranged by size:
2219 2206 1697 617
BsaAI yAC'GTr
Cuts at: 493 1204 3217 4036 4530 493
Size: 711 2013 819 494 2702
Fragments arranged by size:
2702 2013 819 711 494
BsaBI GATnn'nnATC
Cuts at: 3260 6733 3260
Size: 3473 3266
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2679 2805 3756 4546
Size: 53 83 186 936 1146 126 951 790
Cuts at: 4546 4928 5563 275
Size: 382 635 1451
Fragments arranged by size:
1451 1146 951 936 790 635 382 186
126 83 53
BsaWI w'CCGG_w
Cuts at: 3289 5172 5570 5844 5991 6402 3289
Size: 1883 398 274 147 411 3626
Fragments arranged by size:
3626 1883 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2498 2528 3056 3086 1178
Size: 30 165 30 1095 30 528 30 4831
Fragments arranged by size:
4831 1095 528 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2129 2519 3005 3957 1186
Size: 943 390 486 952 3968
Fragments arranged by size:
3968 952 943 486 390
BseYI C'CCAG_C
Cuts at: 2659 2672 3103 3351 3408 5942 6438 2659
Size: 13 431 248 57 2534 496 2960
Fragments arranged by size:
2960 2534 496 431 248 57 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2273 2510 3314 3731 3866 1163
Size: 573 537 237 804 417 135 4036
Fragments arranged by size:
4036 804 573 537 417 237 135
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4189 4950 5978 1540
Size: 275 186 2188 761 1028 2301
Fragments arranged by size:
2301 2188 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2307 2401 2436 2467 2592 3189 3473 3515 3785
Size: 94 35 31 125 597 284 42 270
Cuts at: 3785 4800 4885 5956 2307
Size: 1015 85 1071 3090
Fragments arranged by size:
3090 1071 1015 597 284 270 125 94
85 42 35 31
BsmI GAATG_Cn'
Cuts at: 3913 4250 4343 3913
Size: 337 93 6309
Fragments arranged by size:
6309 337 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2137 2307 2401 2436 2467 2592 2793
Size: 399 347 170 94 35 31 125 201
Cuts at: 2793 3189 3473 3515 3785 4058 4800 4885 5956
Size: 396 284 42 270 273 742 85 1071
Cuts at: 5956 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 396 347 284 273
270 201 170 125 94 85 42 35
31
BspEI T'CCGG_A
Cuts at: 6402 6402
Size: 6739
BspHI T'CATG_A
Cuts at: 4628 6358 4628
Size: 1730 5009
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2033 2532 2937 3633 3783 3832 3913 4094
Size: 1189 499 405 696 150 49 81 181
Cuts at: 4094 844
Size: 3489
Fragments arranged by size:
3489 1189 696 499 405 181 150 81
49
BsrBI CCG'CTC
Cuts at: 1394 1823 2205 2831 2911 3424 4626 1394
Size: 429 382 626 80 513 1202 3507
Fragments arranged by size:
3507 1202 626 513 429 382 80
BsrDI GCAATG_nn'
Cuts at: 65 1248 5234 5408 65
Size: 1183 3986 174 1396
Fragments arranged by size:
3986 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3237 3657 5383 5570 1700
Size: 110 1427 420 1726 187 2869
Fragments arranged by size:
2869 1726 1427 420 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6739
BssHII G'CGCG_C
Cuts at: 1925 2971 1925
Size: 1046 5693
BssSI C'ACGA_G
Cuts at: 4799 5811 4799
Size: 1012 5727
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3901 794
Size: 3107 3632
BstBI TT'CG_AA
Cuts at: 2007 5543 2007
Size: 3536 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6739
BstXI CCAn_nnnn'nTGG
Cuts at: 2122 2122
Size: 6739
BstYI r'GATC_y
Cuts at: 1972 2895 3141 3300 3496 4069 4436 4821 4838
Size: 923 246 159 196 573 367 385 17
Cuts at: 4838 6290 6376 6388 6734 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 923 573 385 367 346 246
196 159 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2643 2793 3751 513
Size: 552 1578 150 958 3501
Fragments arranged by size:
3501 1578 958 552 150
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2613 487
Size: 683 56 1387 4613
Fragments arranged by size:
4613 1387 683 56
BtsI GCAGTG_nn'
Cuts at: 2307 2445 2611 2966 4255 5049 5069 2307
Size: 138 166 355 1289 794 20 3977
Fragments arranged by size:
3977 1289 794 355 166 138 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6739
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6704
DraI TTT'AAA
Cuts at: 4043 4043
Size: 6739
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2477 5746 809
Size: 507 1161 3269 1802
Fragments arranged by size:
3269 1802 1161 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2426 2814 3324
Size: 54 1425 50 275 18 596 388 510
Cuts at: 3324 4186 5075 6704 8
Size: 862 889 1629 43
Fragments arranged by size:
1629 1425 889 862 596 510 388 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4186 1537
Size: 275 2374 4090
Fragments arranged by size:
4090 2374 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4669 1353
Size: 3316 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2861 3620 3964 5326 5700 5846
Size: 1064 138 1272 759 344 1362 374 146
Cuts at: 5846 387
Size: 1280
Fragments arranged by size:
1362 1280 1272 1064 759 374 344 146
138
EcoICRI GAG'CTC
Cuts at: 2465 2590 3187 3471 3513 2465
Size: 125 597 284 42 5691
Fragments arranged by size:
5691 597 284 125 42
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2255 2334 2347 2518 2577 2640 3083
Size: 393 623 79 13 171 59 63 443
Cuts at: 3083 3140 3698 3713 4802 5399 6186 6454 1239
Size: 57 558 15 1089 597 787 268 1524
Fragments arranged by size:
1524 1089 787 623 597 558 443 393
268 171 79 63 59 57 15 13
EcoNI CCTnn'n_nnAGG
Cuts at: 3826 3826
Size: 6739
EcoO109I rG'GnC_Cy
Cuts at: 2134 2509 3058 3622 2134
Size: 375 549 564 5251
Fragments arranged by size:
5251 564 549 375
EcoRI G'AATT_C
Cuts at: 4048 4426 4048
Size: 378 6361
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2125 3754 2125
Size: 1629 5110
FspI TGC'GCA
Cuts at: 5245 5245
Size: 6739
HaeII r_GCGC'y
Cuts at: 1678 3088 3759 3811 5886 1678
Size: 1410 671 52 2075 2531
Fragments arranged by size:
2531 2075 1410 671 52
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2764 2796 5380 5412 5454
Size: 32 135 32 1192 32 2584 32 42
Cuts at: 5454 5486 1373
Size: 32 2626
Fragments arranged by size:
2626 2584 1192 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4083 4329 669
Size: 988 281 2145 246 3079
Fragments arranged by size:
3079 2145 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6739
HpaI GTT'AAC
Cuts at: 4329 4329
Size: 6739
KasI G'GCGC_C
Cuts at: 3755 3755
Size: 6739
KpnI G_GTAC'C
Cuts at: 4064 4064
Size: 6739
MfeI C'AATT_G
Cuts at: 4338 4338
Size: 6739
MluI A'CGCG_T
Cuts at: 6615 6615
Size: 6739
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2426 5853 6037 1305
Size: 1121 3427 184 2007
Fragments arranged by size:
3427 2007 1121 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2428 10
Size: 54 1425 343 596 4321
Fragments arranged by size:
4321 1425 596 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2120 2310 2450 2687 4697 5056 5215
Size: 648 954 190 140 237 2010 359 159
Cuts at: 5215 518
Size: 2042
Fragments arranged by size:
2042 2010 954 648 359 237 190 159
140
MspA1I CmG'CkG
Cuts at: 1606 2477 2575 2795 2826 2998 3036 3322 3850
Size: 871 98 220 31 172 38 286 528
Cuts at: 3850 4155 4832 5980 6225 1606
Size: 305 677 1148 245 2120
Fragments arranged by size:
2120 1148 871 677 528 305 286 245
220 172 98 38 31
NaeI GCC'GGC
Cuts at: 3239 3659 3239
Size: 420 6319
NarI GG'CG_CC
Cuts at: 3756 3756
Size: 6739
NcoI C'CATG_G
Cuts at: 513 1065 2643 513
Size: 552 1578 4609
Fragments arranged by size:
4609 1578 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6739
NgoMIV G'CCGG_C
Cuts at: 3237 3657 3237
Size: 420 6319
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6739
NotI GC'GGCC_GC
Cuts at: 4186 4186
Size: 6739
NspI r_CATG'y
Cuts at: 1132 1229 2983 3392 4097 5642 1132
Size: 97 1754 409 705 1545 2229
Fragments arranged by size:
2229 1754 1545 705 409 97
PasI CC'CwG_GG
Cuts at: 1348 2028 1348
Size: 680 6059
PciI A'CATG_T
Cuts at: 2979 5638 2979
Size: 2659 4080
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2028 2659 1108
Size: 920 631 5188
Fragments arranged by size:
5188 920 631
PfoI T'CCnGG_A
Cuts at: 3117 3117
Size: 6739
PmeI GTTT'AAAC
Cuts at: 4043 4043
Size: 6739
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3056 3088 4808 4840 6321 6353 3056
Size: 32 1720 32 1481 32 3442
Fragments arranged by size:
3442 1720 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2509 3622 2509
Size: 1113 5626
PshAI GACnn'nnGTC
Cuts at: 1613 2882 1613
Size: 1269 5470
PsiI TTA'TAA
Cuts at: 4309 4309
Size: 6739
PspOMI G'GGCC_C
Cuts at: 2133 2133
Size: 6739
PspXI vC'TCGA_Gb
Cuts at: 3543 3543
Size: 6739
PstI C_TGCA'G
Cuts at: 830 2155 3249 3321 3888 4091 830
Size: 1325 1094 72 567 203 3478
Fragments arranged by size:
3478 1325 1094 567 203 72
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6739
PvuII CAG'CTG
Cuts at: 2477 2575 2998 3322 3850 2477
Size: 98 423 324 528 5366
Fragments arranged by size:
5366 528 423 324 98
SacI G_AGCT'C
Cuts at: 2467 2592 3189 3473 3515 2467
Size: 125 597 284 42 5691
Fragments arranged by size:
5691 597 284 125 42
SacII CC_GC'GG
Cuts at: 2796 2796
Size: 6739
SalI G'TCGA_C
Cuts at: 1936 4081 1936
Size: 2145 4594
SbfI CC_TGCA'GG
Cuts at: 4091 4091
Size: 6739
ScaI AGT'ACT
Cuts at: 3155 4987 6699 3155
Size: 1832 1712 3195
Fragments arranged by size:
3195 1832 1712
SfcI C'TryA_G
Cuts at: 826 1045 2151 2545 3245 3317 3884 4087 5222
Size: 219 1106 394 700 72 567 203 1135
Cuts at: 5222 5903 6094 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1106 700 681 567 394 219
203 191 72
SfoI GGC'GCC
Cuts at: 3757 3757
Size: 6739
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6739
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6739
SmaI CCC'GGG
Cuts at: 1872 4066 1872
Size: 2194 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3437 3543 3558 3815 4843 5744
Size: 197 926 1494 106 15 257 1028 901
Cuts at: 5744 6006 820
Size: 262 1553
Fragments arranged by size:
1553 1494 1028 926 901 262 257 197
106 15
SnaBI TAC'GTA
Cuts at: 493 4036 4530 493
Size: 3543 494 2702
Fragments arranged by size:
3543 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6739
SphI G_CATG'C
Cuts at: 1132 3392 4097 1132
Size: 2260 705 3774
Fragments arranged by size:
3774 2260 705
SspI AAT'ATT
Cuts at: 5 52 4663 5
Size: 47 4611 2081
Fragments arranged by size:
4611 2081 47
StuI AGG'CCT
Cuts at: 1517 3047 3233 3821 1517
Size: 1530 186 588 4435
Fragments arranged by size:
4435 1530 588 186
StyI C'CwwG_G
Cuts at: 513 1065 2186 2643 4178 513
Size: 552 1121 457 1535 3074
Fragments arranged by size:
3074 1535 1121 552 457
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3095 4766 4783 4936 5121 5566 325
Size: 1255 1515 1671 17 153 185 445 1498
Fragments arranged by size:
1671 1515 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3153 3219 3645
Size: 274 80 33 51 1443 1176 66 426
Cuts at: 3645 4985 6697 96
Size: 1340 1712 138
Fragments arranged by size:
1712 1443 1340 1176 426 274 138 80
66 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2213 2245 6699 6731 2213
Size: 32 4454 32 2221
Fragments arranged by size:
4454 2221 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2636 1316
Size: 1320 5419
XbaI T'CTAG_A
Cuts at: 4075 4075
Size: 6739
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3178 3400 3178
Size: 222 6517
XhoI C'TCGA_G
Cuts at: 1943 3543 1943
Size: 1600 5139
XmaI C'CCGG_G
Cuts at: 1870 4064 1870
Size: 2194 4545
XmnI GAAnn'nnTTC
Cuts at: 2078 3047 3737 4868 2078
Size: 969 690 1131 3949
Fragments arranged by size:
3949 1131 969 690
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2680 4547 276
Size: 53 83 186 936 1146 1867 2468
Fragments arranged by size:
2468 1867 1146 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SacI SacII SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoRV FseI FspAI NruI NsiI PacI PmlI PsrI
RsrII SanDI SapI SexAI SfiI SrfI SwaI