Restriction map for
pFN21ASDA1100
(Circular) (Six-base) MAPSORT of: pFN21ASDA1100.seq Check: 535 from: 1 to: 6025
pFN21ASDA1100 6025 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2308 2308
Size: 6025
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3835 278
Size: 53 83 186 936 2299 2468
Fragments arranged by size:
2468 2299 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3368 1937
Size: 1431 4594
Acc65I G'GTAC_C
Cuts at: 3346 3346
Size: 6025
AclI AA'CG_TT
Cuts at: 1563 4152 4525 1563
Size: 2589 373 3063
Fragments arranged by size:
3063 2589 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3157 4088 5472 5740 1632
Size: 1525 931 1384 268 1917
Fragments arranged by size:
1917 1525 1384 931 268
AfeI AGC'GCT
Cuts at: 2177 2177
Size: 6025
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5828
AflIII A'CryG_T
Cuts at: 1203 3269 4924 5901 1203
Size: 2066 1655 977 1327
Fragments arranged by size:
2066 1655 1327 977
AgeI A'CCGG_T
Cuts at: 4856 4856
Size: 6025
AhdI GACnn_n'nnGTC
Cuts at: 4754 4754
Size: 6025
AleI CACnn'nnGTG
Cuts at: 1166 2360 1166
Size: 1194 4831
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6025
AlwNI CAG_nnn'CTG
Cuts at: 2515 2809 2884 3157 3768 5340 2515
Size: 294 75 273 611 1572 3200
Fragments arranged by size:
3200 1572 611 294 273 75
ApaI G_GGCC'C
Cuts at: 2147 2985 2147
Size: 838 5187
ApaLI G'TGCA_C
Cuts at: 2321 2500 4082 5238 2321
Size: 179 1582 1156 3108
Fragments arranged by size:
3108 1582 1156 179
ApoI r'AATT_y
Cuts at: 1493 3334 3560 3712 5821 1493
Size: 1841 226 152 2109 1697
Fragments arranged by size:
2109 1841 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4579 160
Size: 872 3547 1606
Fragments arranged by size:
3547 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6025
AvaI C'yCGr_G
Cuts at: 1870 1943 2110 2138 2166 3078 3350 1870
Size: 73 167 28 28 912 272 4545
Fragments arranged by size:
4545 912 272 167 73 28 28
BamHI G'GATC_C
Cuts at: 3355 3722 3355
Size: 367 5658
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2614 2767 3301 3346 4801
Size: 325 217 627 827 153 534 45 1455
Cuts at: 4801 618
Size: 1842
Fragments arranged by size:
1842 1455 827 627 534 325 217 153
45
BanII G_rGCy'C
Cuts at: 1391 2038 2147 2261 2294 2985 3344 1391
Size: 647 109 114 33 691 359 4072
Fragments arranged by size:
4072 691 647 359 114 109 33
BbeI G_GCGC'C
Cuts at: 2618 2618
Size: 6025
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5054
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4216 4250 1903
Size: 34 2279 34 3678
Fragments arranged by size:
3678 2279 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3917 5127 3917
Size: 1210 4815
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6025
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2142 2623 4636
Size: 107 122 71 631 605 470 481 2013
Cuts at: 4636 136
Size: 1525
Fragments arranged by size:
2013 1525 631 605 481 470 122 107
71
BglII A'GATC_T
Cuts at: 6020 6020
Size: 6025
BlpI GC'TnA_GC
Cuts at: 3442 3442
Size: 6025
Bme1580I G_kGCm'C
Cuts at: 1790 2147 2325 2419 2504 2526 2772 2985 3306
Size: 357 178 94 85 22 246 213 321
Cuts at: 3306 4086 5242 1790
Size: 780 1156 2573
Fragments arranged by size:
2573 1156 780 357 321 246 213 178
94 85 22
BmgBI CAC'GTC
Cuts at: 1318 2686 3007 3270 1318
Size: 1368 321 263 4073
Fragments arranged by size:
4073 1368 321 263
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3756 4714 442
Size: 416 53 83 399 24 2339 958 1753
Fragments arranged by size:
2339 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6025
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2536 2651 4685 1239
Size: 1297 115 2034 2579
Fragments arranged by size:
2579 2034 1297 115
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4150 5015 5313 4150
Size: 865 298 4862
Fragments arranged by size:
4862 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3089 4688 882
Size: 617 1590 1599 2219
Fragments arranged by size:
2219 1599 1590 617
BsaAI yAC'GTr
Cuts at: 493 1204 3322 3816 493
Size: 711 2118 494 2702
Fragments arranged by size:
2702 2118 711 494
BsaBI GATnn'nnATC
Cuts at: 6019 6019
Size: 6025
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2615 3291 3832 4214
Size: 53 83 186 936 1082 676 541 382
Cuts at: 4214 4849 275
Size: 635 1451
Fragments arranged by size:
1451 1082 936 676 635 541 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3264 4458 4856 5130 5277 5688 3264
Size: 1194 398 274 147 411 3601
Fragments arranged by size:
3601 1194 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2534 2564 2693 2723 3302
Size: 30 165 30 1131 30 129 30 579
Cuts at: 3302 3332 1178
Size: 30 3871
Fragments arranged by size:
3871 1131 579 165 129 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2240 1186
Size: 1054 4971
BseYI C'CCAG_C
Cuts at: 2187 2275 2283 2418 2428 2598 2646 2749 2927
Size: 88 8 135 10 170 48 103 178
Cuts at: 2927 3061 5228 5724 2187
Size: 134 2167 496 2488
Fragments arranged by size:
2488 2167 496 178 170 135 134 103
88 48 10 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2255 2555 2759 2783 1163
Size: 573 519 300 204 24 4405
Fragments arranged by size:
4405 573 519 300 204 24
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3475 4236 5264 1540
Size: 275 186 1474 761 1028 2301
Fragments arranged by size:
2301 1474 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2325 2504 2858 4086 4171 5242 2325
Size: 179 354 1228 85 1071 3108
Fragments arranged by size:
3108 1228 1071 354 179 85
BsmI GAATG_Cn'
Cuts at: 2271 3536 3629 2271
Size: 1265 93 4667
Fragments arranged by size:
4667 1265 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2038 2147 2261 2294 2325 2419 2504
Size: 399 248 109 114 33 31 94 85
Cuts at: 2504 2526 2772 2858 2985 3306 3344 4086 4171
Size: 22 246 86 127 321 38 742 85
Cuts at: 4171 5242 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 321 248 246 127
114 109 94 86 85 85 38 33
31 22
BspEI T'CCGG_A
Cuts at: 5688 5688
Size: 6025
BspHI T'CATG_A
Cuts at: 3914 5644 3914
Size: 1730 4295
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2308 2700 2790 3380 844
Size: 1464 392 90 590 3489
Fragments arranged by size:
3489 1464 590 392 90
BsrBI CCG'CTC
Cuts at: 1394 1823 3058 3912 1394
Size: 429 1235 854 3507
Fragments arranged by size:
3507 1235 854 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2982 4520 4694 65
Size: 1183 1734 1538 174 1396
Fragments arranged by size:
1734 1538 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2249 2584 4669 4856 1700
Size: 110 439 335 2085 187 2869
Fragments arranged by size:
2869 2085 439 335 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6025
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6025
BssSI C'ACGA_G
Cuts at: 2312 4085 5097 2312
Size: 1773 1012 3240
Fragments arranged by size:
3240 1773 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2515 2782 2884 794
Size: 1721 267 102 3935
Fragments arranged by size:
3935 1721 267 102
BstBI TT'CG_AA
Cuts at: 2007 4829 2007
Size: 2822 3203
BstEII G'GTnAC_C
Cuts at: 1184 2353 1184
Size: 1169 4856
BstXI CCAn_nnnn'nTGG
Cuts at: 2611 2995 2611
Size: 384 5641
BstYI r'GATC_y
Cuts at: 1972 3355 3722 4107 4124 5576 5662 5674 6020
Size: 1383 367 385 17 1452 86 12 346
Cuts at: 6020 1972
Size: 1977
Fragments arranged by size:
1977 1452 1383 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2030 2070 2843 513
Size: 552 965 40 773 3695
Fragments arranged by size:
3695 965 773 552 40
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5286
Fragments arranged by size:
5286 683 56
BtsI GCAGTG_nn'
Cuts at: 2049 2419 2882 3541 4335 4355 2049
Size: 370 463 659 794 20 3719
Fragments arranged by size:
3719 794 659 463 370 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6025
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5990
DraI TTT'AAA
Cuts at: 3329 3329
Size: 6025
DraIII CAC_nnn'GTG
Cuts at: 3237 3237
Size: 6025
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5032 809
Size: 507 3716 1802
Fragments arranged by size:
3716 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2054 2151 3051
Size: 54 1425 50 275 18 224 97 900
Cuts at: 3051 3472 4361 5990 8
Size: 421 889 1629 43
Fragments arranged by size:
1629 1425 900 889 421 275 224 97
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3472 1537
Size: 275 1660 4090
Fragments arranged by size:
4090 1660 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3955 1353
Size: 2602 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2053 4612 4986 5132 387
Size: 1064 138 464 2559 374 146 1280
Fragments arranged by size:
2559 1280 1064 464 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2536 2651 3157 4088 4685 5472 5740
Size: 393 904 115 506 931 597 787 268
Cuts at: 5740 1239
Size: 1524
Fragments arranged by size:
1524 931 904 787 597 506 393 268
115
EcoO109I rG'GnC_Cy
Cuts at: 2096 2143 2144 2330 2981 3037 2096
Size: 47 1 186 651 56 5084
Fragments arranged by size:
5084 651 186 56 47 1
EcoRI G'AATT_C
Cuts at: 3334 3712 3334
Size: 378 5647
FspI TGC'GCA
Cuts at: 4531 4531
Size: 6025
HaeII r_GCGC'y
Cuts at: 1678 2179 2233 2618 5172 1678
Size: 501 54 385 2554 2531
Fragments arranged by size:
2554 2531 501 385 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2693 2725 4666 4698 4740
Size: 32 135 32 1121 32 1941 32 42
Cuts at: 4740 4772 1373
Size: 32 2626
Fragments arranged by size:
2626 1941 1121 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3244 3369 3615 669
Size: 988 281 1306 125 246 3079
Fragments arranged by size:
3079 1306 988 281 246 125
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6025
HpaI GTT'AAC
Cuts at: 3615 3615
Size: 6025
KasI G'GCGC_C
Cuts at: 2614 2614
Size: 6025
KpnI G_GTAC'C
Cuts at: 3350 3350
Size: 6025
MfeI C'AATT_G
Cuts at: 3624 3624
Size: 6025
MluI A'CGCG_T
Cuts at: 5901 5901
Size: 6025
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3126 5139 5323 1305
Size: 1821 2013 184 2007
Fragments arranged by size:
2013 2007 1821 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2056 2153 10
Size: 54 1425 343 224 97 3882
Fragments arranged by size:
3882 1425 343 224 97 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2360 2609 3983 4342 4501 518
Size: 648 1194 249 1374 359 159 2042
Fragments arranged by size:
2042 1374 1194 648 359 249 159
MspA1I CmG'CkG
Cuts at: 1606 2080 2515 2671 2797 3196 3441 4118 5266
Size: 474 435 156 126 399 245 677 1148
Cuts at: 5266 5511 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 474 435 399 245 245
156 126
NaeI GCC'GGC
Cuts at: 2251 2251
Size: 6025
NarI GG'CG_CC
Cuts at: 2615 2615
Size: 6025
NcoI C'CATG_G
Cuts at: 513 1065 2030 513
Size: 552 965 4508
Fragments arranged by size:
4508 965 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6025
NgoMIV G'CCGG_C
Cuts at: 2249 2249
Size: 6025
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6025
NotI GC'GGCC_GC
Cuts at: 3472 3472
Size: 6025
NspI r_CATG'y
Cuts at: 1132 1229 2436 3383 4928 1132
Size: 97 1207 947 1545 2229
Fragments arranged by size:
2229 1545 1207 947 97
PasI CC'CwG_GG
Cuts at: 1348 2620 2713 3306 1348
Size: 1272 93 593 4067
Fragments arranged by size:
4067 1272 593 93
PciI A'CATG_T
Cuts at: 4924 4924
Size: 6025
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6025
PmeI GTTT'AAAC
Cuts at: 3329 3329
Size: 6025
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4094 4126 5607 5639 4094
Size: 32 1481 32 4480
Fragments arranged by size:
4480 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2096 2330 2096
Size: 234 5791
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6025
PsiI TTA'TAA
Cuts at: 3595 3595
Size: 6025
PspOMI G'GGCC_C
Cuts at: 2143 2981 2143
Size: 838 5187
PspXI vC'TCGA_Gb
Cuts at: 2138 2138
Size: 6025
PstI C_TGCA'G
Cuts at: 830 2414 2643 2877 3377 830
Size: 1584 229 234 500 3478
Fragments arranged by size:
3478 1584 500 234 229
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6025
PvuII CAG'CTG
Cuts at: 2515 3196 2515
Size: 681 5344
SalI G'TCGA_C
Cuts at: 1936 3367 1936
Size: 1431 4594
SbfI CC_TGCA'GG
Cuts at: 3377 3377
Size: 6025
ScaI AGT'ACT
Cuts at: 4273 5985 4273
Size: 1712 4313
SfcI C'TryA_G
Cuts at: 826 1045 2388 2410 2639 2873 3373 4508 5189
Size: 219 1343 22 229 234 500 1135 681
Cuts at: 5189 5380 826
Size: 191 1471
Fragments arranged by size:
1471 1343 1135 681 500 234 229 219
191 22
SfiI GGCCn_nnn'nGGCC
Cuts at: 2142 2142
Size: 6025
SfoI GGC'GCC
Cuts at: 2616 2616
Size: 6025
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6025
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6025
SmaI CCC'GGG
Cuts at: 1872 2112 2168 3080 3352 1872
Size: 240 56 912 272 4545
Fragments arranged by size:
4545 912 272 240 56
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2138 4129 5030 5292 820
Size: 197 926 195 1991 901 262 1553
Fragments arranged by size:
1991 1553 926 901 262 197 195
SnaBI TAC'GTA
Cuts at: 493 3322 3816 493
Size: 2829 494 2702
Fragments arranged by size:
2829 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6025
SphI G_CATG'C
Cuts at: 1132 2436 3383 1132
Size: 1304 947 3774
Fragments arranged by size:
3774 1304 947
SspI AAT'ATT
Cuts at: 5 52 3949 5
Size: 47 3897 2081
Fragments arranged by size:
3897 2081 47
StuI AGG'CCT
Cuts at: 1517 2534 1517
Size: 1017 5008
StyI C'CwwG_G
Cuts at: 513 1065 2030 2371 3464 513
Size: 552 965 341 1093 3074
Fragments arranged by size:
3074 1093 965 552 341
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2289 2297 2621 2873 4052 4069 4222
Size: 1255 709 8 324 252 1179 17 153
Cuts at: 4222 4407 4852 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1179 709 445 324 252 185
153 17 8
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4271 5983 96
Size: 274 80 33 51 1443 2294 1712 138
Fragments arranged by size:
2294 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2534 2566 5985 6017 2534
Size: 32 3419 32 2542
Fragments arranged by size:
3419 2542 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6025
XbaI T'CTAG_A
Cuts at: 3361 3361
Size: 6025
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2402 2992 2402
Size: 590 5435
XhoI C'TCGA_G
Cuts at: 1943 2138 1943
Size: 195 5830
XmaI C'CCGG_G
Cuts at: 1870 2110 2166 3078 3350 1870
Size: 240 56 912 272 4545
Fragments arranged by size:
4545 912 272 240 56
XmnI GAAnn'nnTTC
Cuts at: 4154 4154
Size: 6025
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3833 276
Size: 53 83 186 936 2299 2468
Fragments arranged by size:
2468 2299 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SalI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI
FseI FspAI NruI NsiI PacI PfoI PmlI PsrI
RsrII SacI SacII SanDI SapI SexAI SrfI SwaI