Restriction map for
pFN21ASDA1095
(Circular) (Six-base) MAPSORT of: pFN21ASDA1095.seq Check: 3473 from: 1 to: 7927
pFN21ASDA1095 7927 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3013 3851 3013
Size: 838 7089
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5737 278
Size: 53 83 186 936 4201 2468
Fragments arranged by size:
4201 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5190 5220 5270 1937
Size: 3253 30 50 4594
Fragments arranged by size:
4594 3253 50 30
Acc65I G'GTAC_C
Cuts at: 5248 5248
Size: 7927
AclI AA'CG_TT
Cuts at: 1563 6054 6427 1563
Size: 4491 373 3063
Fragments arranged by size:
4491 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2090 3865 4520 4886 5990 7374 7642 1632
Size: 458 1775 655 366 1104 1384 268 1917
Fragments arranged by size:
1917 1775 1384 1104 655 458 366 268
AfeI AGC'GCT
Cuts at: 2333 2517 3956 2333
Size: 184 1439 6304
Fragments arranged by size:
6304 1439 184
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7730
AflIII A'CryG_T
Cuts at: 1203 2418 6826 7803 1203
Size: 1215 4408 977 1327
Fragments arranged by size:
4408 1327 1215 977
AgeI A'CCGG_T
Cuts at: 6758 6758
Size: 7927
AhdI GACnn_n'nnGTC
Cuts at: 6656 6656
Size: 7927
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7927
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4241 1184
Size: 3057 4870
AlwNI CAG_nnn'CTG
Cuts at: 2314 2383 2718 4525 4585 4966 5670 7242 2314
Size: 69 335 1807 60 381 704 1572 2999
Fragments arranged by size:
2999 1807 1572 704 381 335 69 60
ApaLI G'TGCA_C
Cuts at: 5984 7140 5984
Size: 1156 6771
ApoI r'AATT_y
Cuts at: 1493 2678 2919 5236 5462 5614 7723 1493
Size: 1185 241 2317 226 152 2109 1697
Fragments arranged by size:
2317 2109 1697 1185 241 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3441 6481 160
Size: 872 2409 3040 1606
Fragments arranged by size:
3040 2409 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7927
AvaI C'yCGr_G
Cuts at: 1870 1943 2327 4285 4387 5252 1870
Size: 73 384 1958 102 865 4545
Fragments arranged by size:
4545 1958 865 384 102 73
BamHI G'GATC_C
Cuts at: 3166 3933 5257 5624 3166
Size: 767 1324 367 5469
Fragments arranged by size:
5469 1324 767 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4475 5248 6703 618
Size: 325 217 627 2688 773 1455 1842
Fragments arranged by size:
2688 1842 1455 773 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2217 3012 3773 3969 3978 4242 4260 4568
Size: 826 795 761 196 9 264 18 308
Cuts at: 4568 4754 5246 1391
Size: 186 492 4072
Fragments arranged by size:
4072 826 795 761 492 308 264 196
186 18 9
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2122 3354 3678 928
Size: 971 223 1232 324 5177
Fragments arranged by size:
5177 1232 971 324 223
BbvCI CC'TCA_GC
Cuts at: 3196 3196
Size: 7927
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6118 6152 1903
Size: 34 4181 34 3678
Fragments arranged by size:
4181 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2881 5819 7029 2881
Size: 2938 1210 3779
Fragments arranged by size:
3779 2938 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7927
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2454 2627 6538
Size: 107 122 71 631 605 782 173 3911
Cuts at: 6538 136
Size: 1525
Fragments arranged by size:
3911 1525 782 631 605 173 122 107
71
BglII A'GATC_T
Cuts at: 4338 7922 4338
Size: 3584 4343
BlpI GC'TnA_GC
Cuts at: 2711 5344 2711
Size: 2633 5294
Bme1580I G_kGCm'C
Cuts at: 1790 4478 5988 7144 1790
Size: 2688 1510 1156 2573
Fragments arranged by size:
2688 2573 1510 1156
BmgBI CAC'GTC
Cuts at: 1318 2056 2128 2224 2398 4225 1318
Size: 738 72 96 174 1827 5020
Fragments arranged by size:
5020 1827 738 174 96 72
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5658 6616 442
Size: 416 53 83 399 24 4241 958 1753
Fragments arranged by size:
4241 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3645 1056
Size: 2589 5338
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3211 3211
Size: 7927
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2117 2525 3287 3293 3521 3626 3992 4097
Size: 878 408 762 6 228 105 366 105
Cuts at: 4097 4133 4355 4960 6587 1239
Size: 36 222 605 1627 2579
Fragments arranged by size:
2579 1627 878 762 605 408 366 228
222 105 105 36 6
Bpu10I CC'TnA_GC
Cuts at: 3196 4121 4748 3196
Size: 925 627 6375
Fragments arranged by size:
6375 925 627
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2219 2234 2558 2663 3962 4139 4378 5015 6052
Size: 15 324 105 1299 177 239 637 1037
Cuts at: 6052 6917 7215 2219
Size: 865 298 2931
Fragments arranged by size:
2931 1299 1037 865 637 324 298 239
177 105 15
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3417 6590 882
Size: 617 1918 3173 2219
Fragments arranged by size:
3173 2219 1918 617
BsaAI yAC'GTr
Cuts at: 493 1204 5224 5718 493
Size: 711 4020 494 2702
Fragments arranged by size:
4020 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 3171 3365 5138 7921 3171
Size: 194 1773 2783 3177
Fragments arranged by size:
3177 2783 1773 194
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3798 3918 4038 5734
Size: 53 83 186 936 2265 120 120 1696
Cuts at: 5734 6116 6751 275
Size: 382 635 1451
Fragments arranged by size:
2265 1696 1451 936 635 382 186 120
120 83 53
BsaWI w'CCGG_w
Cuts at: 3333 6360 6758 7032 7179 7590 3333
Size: 3027 398 274 147 411 3670
Fragments arranged by size:
3670 3027 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2777 2807 3965 3995 4589
Size: 30 165 30 1374 30 1158 30 594
Cuts at: 4589 4619 1178
Size: 30 4486
Fragments arranged by size:
4486 1374 1158 594 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3200 3275 3623 4375 4964 1186
Size: 2014 75 348 752 589 4149
Fragments arranged by size:
4149 2014 752 589 348 75
BseYI C'CCAG_C
Cuts at: 2594 2622 3149 3313 4574 4585 4653 7130 7626
Size: 28 527 164 1261 11 68 2477 496
Cuts at: 7626 2594
Size: 2895
Fragments arranged by size:
2895 2477 1261 527 496 164 68 28
11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2183 2546 3041 3476 3575 3895 4169
Size: 573 447 363 495 435 99 320 274
Cuts at: 4169 1163
Size: 4921
Fragments arranged by size:
4921 573 495 447 435 363 320 274
99
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2197 2293 2825 3906 4698 5377
Size: 275 186 196 96 532 1081 792 679
Cuts at: 5377 6138 7166 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1081 1028 792 761 679 532 275
196 186 96
BsiHKAI G_wGCw'C
Cuts at: 2217 2325 3755 3773 3969 3978 4242 4260 4754
Size: 108 1430 18 196 9 264 18 494
Cuts at: 4754 5988 6073 7144 2217
Size: 1234 85 1071 3000
Fragments arranged by size:
3000 1430 1234 1071 494 264 196 108
85 18 18 9
BsiWI C'GTAC_G
Cuts at: 2271 2271
Size: 7927
BsmI GAATG_Cn'
Cuts at: 5438 5531 5438
Size: 93 7834
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2217 2325 3012 3755 3773 3969 3978
Size: 399 427 108 687 743 18 196 9
Cuts at: 3978 4242 4260 4478 4568 4754 5246 5988 6073
Size: 264 18 218 90 186 492 742 85
Cuts at: 6073 7144 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 743 742 687 492 427 399
264 218 196 186 108 90 85 18
18 9
BspEI T'CCGG_A
Cuts at: 7590 7590
Size: 7927
BspHI T'CATG_A
Cuts at: 2923 2971 5004 5816 7546 2923
Size: 48 2033 812 1730 3304
Fragments arranged by size:
3304 2033 1730 812 48
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3013 3597 3851 3882 4608 5007 5282 844
Size: 2169 584 254 31 726 399 275 3489
Fragments arranged by size:
3489 2169 726 584 399 275 254 31
BsrBI CCG'CTC
Cuts at: 1394 1823 2233 4327 4367 4540 5814 1394
Size: 429 410 2094 40 173 1274 3507
Fragments arranged by size:
3507 2094 1274 429 410 173 40
BsrDI GCAATG_nn'
Cuts at: 65 1248 3390 3512 6422 6596 65
Size: 1183 2142 122 2910 174 1396
Fragments arranged by size:
2910 2142 1396 1183 174 122
BsrFI r'CCGG_y
Cuts at: 1700 1810 2138 2820 4040 6571 6758 1700
Size: 110 328 682 1220 2531 187 2869
Fragments arranged by size:
2869 2531 1220 682 328 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7927
BssHII G'CGCG_C
Cuts at: 1925 2076 2183 2351 2488 2508 2535 2556 2590
Size: 151 107 168 137 20 27 21 34
Cuts at: 2590 2694 2696 4132 4858 1925
Size: 104 2 1436 726 4994
Fragments arranged by size:
4994 1436 726 168 151 137 107 104
34 27 21 20 2
BssSI C'ACGA_G
Cuts at: 3716 4235 5987 6999 3716
Size: 519 1752 1012 4644
Fragments arranged by size:
4644 1752 1012 519
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2314 4383 794
Size: 1520 2069 4338
Fragments arranged by size:
4338 2069 1520
BstBI TT'CG_AA
Cuts at: 2007 6731 2007
Size: 4724 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7927
BstXI CCAn_nnnn'nTGG
Cuts at: 3443 3820 3443
Size: 377 7550
BstYI r'GATC_y
Cuts at: 1972 3166 3933 4338 4462 4794 4869 5257 5624
Size: 1194 767 405 124 332 75 388 367
Cuts at: 5624 6009 6026 7478 7564 7576 7922 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1194 767 405 388 385 367
346 332 124 86 75 17 12
BstZ17I GTA'TAC
Cuts at: 5191 5221 5191
Size: 30 7897
BtgI C'CryG_G
Cuts at: 513 1065 2022 2191 2631 4413 4698 5158 513
Size: 552 957 169 440 1782 285 460 3282
Fragments arranged by size:
3282 1782 957 552 460 440 285 169
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7188
Fragments arranged by size:
7188 683 56
BtsI GCAGTG_nn'
Cuts at: 2500 5443 6237 6257 2500
Size: 2943 794 20 4170
Fragments arranged by size:
4170 2943 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7927
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2351 2386 529
Size: 35 1787 35 6070
Fragments arranged by size:
6070 1787 35 35
DraI TTT'AAA
Cuts at: 5231 5231
Size: 7927
DraIII CAC_nnn'GTG
Cuts at: 2118 3327 4969 2118
Size: 1209 1642 5076
Fragments arranged by size:
5076 1642 1209
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6934 809
Size: 507 5618 1802
Fragments arranged by size:
5618 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2122 2205 2290
Size: 54 1425 50 275 18 292 83 85
Cuts at: 2290 2374 2432 2497 2628 2724 2818 2822 3903
Size: 84 58 65 131 96 94 4 1081
Cuts at: 3903 4695 5374 6263 7892 8
Size: 792 679 889 1629 43
Fragments arranged by size:
1629 1425 1081 889 792 679 292 275
131 96 94 85 84 83 65 58
54 50 43 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 2290 2822 3903 4695 5374 1537
Size: 275 478 532 1081 792 679 4090
Fragments arranged by size:
4090 1081 792 679 532 478 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3462 3486 3986 4086 4872 5099 5857 1353
Size: 2109 24 500 100 786 227 758 3423
Fragments arranged by size:
3423 2109 786 758 500 227 100 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3754 4385 6514 6888 7034 387
Size: 1064 138 2165 631 2129 374 146 1280
Fragments arranged by size:
2165 2129 1280 1064 631 374 146 138
EcoICRI GAG'CTC
Cuts at: 2215 3771 3967 3976 4240 4258 4752 2215
Size: 1556 196 9 264 18 494 5390
Fragments arranged by size:
5390 1556 494 264 196 18 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2090 2117 2525 3287 3293 3521 3626
Size: 393 458 27 408 762 6 228 105
Cuts at: 3626 3865 3992 4097 4133 4355 4520 4886 4960
Size: 239 127 105 36 222 165 366 74
Cuts at: 4960 5990 6587 7374 7642 1239
Size: 1030 597 787 268 1524
Fragments arranged by size:
1524 1030 787 762 597 458 408 393
366 268 239 228 222 165 127 105
105 74 36 27 6
EcoNI CCTnn'n_nnAGG
Cuts at: 4970 4970
Size: 7927
EcoO109I rG'GnC_Cy
Cuts at: 2092 2101 4744 2092
Size: 9 2643 5275
Fragments arranged by size:
5275 2643 9
EcoRI G'AATT_C
Cuts at: 5236 5614 5236
Size: 378 7549
EcoRV GAT'ATC
Cuts at: 4249 4249
Size: 7927
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5137 5137
Size: 7927
FseI GG_CCGG'CC
Cuts at: 2824 2824
Size: 7927
FspI TGC'GCA
Cuts at: 4100 4229 6433 4100
Size: 129 2204 5594
Fragments arranged by size:
5594 2204 129
HaeII r_GCGC'y
Cuts at: 1678 2242 2335 2519 2573 2588 3958 3999 4293
Size: 564 93 184 54 15 1370 41 294
Cuts at: 4293 4596 4936 7074 1678
Size: 303 340 2138 2531
Fragments arranged by size:
2531 2138 1370 564 340 303 294 184
93 54 41 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2777 2809 2834 2866 3130
Size: 32 135 32 1205 32 25 32 264
Cuts at: 3130 3162 3755 3787 4224 4256 4736 4768 6568
Size: 32 593 32 437 32 480 32 1800
Cuts at: 6568 6600 6642 6674 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1800 1205 593 480 437 264 135
42 32 32 32 32 32 32 32
32 32 32 25
HincII GTy'rAC
Cuts at: 669 1657 1938 2884 2944 5271 5517 669
Size: 988 281 946 60 2327 246 3079
Fragments arranged by size:
3079 2327 988 946 281 246 60
HindIII A'AGCT_T
Cuts at: 748 2988 748
Size: 2240 5687
HpaI GTT'AAC
Cuts at: 5517 5517
Size: 7927
KpnI G_GTAC'C
Cuts at: 5252 5252
Size: 7927
MfeI C'AATT_G
Cuts at: 5526 5526
Size: 7927
MluI A'CGCG_T
Cuts at: 2418 7803 2418
Size: 5385 2542
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3732 3837 4296 4746 5001 7041 7225 1305
Size: 2427 105 459 450 255 2040 184 2007
Fragments arranged by size:
2427 2040 2007 459 450 255 184 105
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6105
Fragments arranged by size:
6105 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3017 3137 3599 4223 5885 6244 6403
Size: 648 1851 120 462 624 1662 359 159
Cuts at: 6403 518
Size: 2042
Fragments arranged by size:
2042 1851 1662 648 624 462 359 159
120
MspA1I CmG'CkG
Cuts at: 1606 2107 2175 2193 2314 2445 2626 2633 2718
Size: 501 68 18 121 131 181 7 85
Cuts at: 2718 3079 3541 3729 3820 3867 4194 4383 4678
Size: 361 462 188 91 47 327 189 295
Cuts at: 4678 4887 4987 5343 6020 7168 7413 1606
Size: 209 100 356 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 501 462 361 356 327
295 245 209 189 188 181 131 121
100 91 85 68 47 18 7
NaeI GCC'GGC
Cuts at: 2140 2822 4042 2140
Size: 682 1220 6025
Fragments arranged by size:
6025 1220 682
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 6418
Fragments arranged by size:
6418 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7927
NgoMIV G'CCGG_C
Cuts at: 2138 2820 4040 2138
Size: 682 1220 6025
Fragments arranged by size:
6025 1220 682
NheI G'CTAG_C
Cuts at: 1052 3641 1052
Size: 2589 5338
NotI GC'GGCC_GC
Cuts at: 5374 5374
Size: 7927
NspI r_CATG'y
Cuts at: 1132 1229 2413 3604 4675 4738 5285 6830 1132
Size: 97 1184 1191 1071 63 547 1545 2229
Fragments arranged by size:
2229 1545 1191 1184 1071 547 97 63
PasI CC'CwG_GG
Cuts at: 1348 2154 2797 1348
Size: 806 643 6478
Fragments arranged by size:
6478 806 643
PciI A'CATG_T
Cuts at: 6826 6826
Size: 7927
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2983 4525 4585 1108
Size: 1875 1542 60 4450
Fragments arranged by size:
4450 1875 1542 60
PfoI T'CCnGG_A
Cuts at: 2094 3969 4175 4260 4872 2094
Size: 1875 206 85 612 5149
Fragments arranged by size:
5149 1875 612 206 85
PmeI GTTT'AAAC
Cuts at: 5231 5231
Size: 7927
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5996 6028 7509 7541 5996
Size: 32 1481 32 6382
Fragments arranged by size:
6382 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2092 2101 4744 2092
Size: 9 2643 5275
Fragments arranged by size:
5275 2643 9
PshAI GACnn'nnGTC
Cuts at: 1613 2771 1613
Size: 1158 6769
PsiI TTA'TAA
Cuts at: 5497 5497
Size: 7927
PspXI vC'TCGA_Gb
Cuts at: 2327 4285 2327
Size: 1958 5969
PstI C_TGCA'G
Cuts at: 830 2310 2652 2899 3654 3848 5279 830
Size: 1480 342 247 755 194 1431 3478
Fragments arranged by size:
3478 1480 1431 755 342 247 194
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7927
PvuII CAG'CTG
Cuts at: 2175 2314 2626 3079 3541 4383 4678 2175
Size: 139 312 453 462 842 295 5424
Fragments arranged by size:
5424 842 462 453 312 295 139
RsrII CG'GwC_CG
Cuts at: 3741 3741
Size: 7927
SacI G_AGCT'C
Cuts at: 2217 3773 3969 3978 4242 4260 4754 2217
Size: 1556 196 9 264 18 494 5390
Fragments arranged by size:
5390 1556 494 264 196 18 9
SacII CC_GC'GG
Cuts at: 2194 2634 2194
Size: 440 7487
SalI G'TCGA_C
Cuts at: 1936 5269 1936
Size: 3333 4594
SapI GCTCTTCn'nnn_
Cuts at: 3486 3986 4086 4872 3486
Size: 500 100 786 6541
Fragments arranged by size:
6541 786 500 100
SbfI CC_TGCA'GG
Cuts at: 5279 5279
Size: 7927
ScaI AGT'ACT
Cuts at: 6175 7887 6175
Size: 1712 6215
SexAI A'CCwGG_T
Cuts at: 4965 4965
Size: 7927
SfcI C'TryA_G
Cuts at: 826 1045 2306 2648 2895 3289 3650 3844 4486
Size: 219 1261 342 247 394 361 194 642
Cuts at: 4486 5275 6410 7091 7282 826
Size: 789 1135 681 191 1471
Fragments arranged by size:
1471 1261 1135 789 681 642 394 361
342 247 219 194 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2627 2627
Size: 7927
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7927
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7927
SmaI CCC'GGG
Cuts at: 1872 5254 1872
Size: 3382 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2234 2249 2327 2573 2642 3977
Size: 197 926 291 15 78 246 69 1335
Cuts at: 3977 4154 4285 4357 5030 6031 6932 7194 820
Size: 177 131 72 673 1001 901 262 1553
Fragments arranged by size:
1553 1335 1001 926 901 673 291 262
246 197 177 131 78 72 69 15
SnaBI TAC'GTA
Cuts at: 493 5224 5718 493
Size: 4731 494 2702
Fragments arranged by size:
4731 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7927
SphI G_CATG'C
Cuts at: 1132 5285 1132
Size: 4153 3774
SspI AAT'ATT
Cuts at: 5 52 2809 5851 5
Size: 47 2757 3042 2081
Fragments arranged by size:
3042 2757 2081 47
StuI AGG'CCT
Cuts at: 1517 2913 3529 1517
Size: 1396 616 5915
Fragments arranged by size:
5915 1396 616
StyI C'CwwG_G
Cuts at: 513 1065 2022 2901 3003 3041 3399 3858 4977
Size: 552 957 879 102 38 358 459 1119
Cuts at: 4977 5366 513
Size: 389 3074
Fragments arranged by size:
3074 1119 957 879 552 459 389 358
102 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2146 3758 5954 5971 6124 6309 6754
Size: 1255 566 1612 2196 17 153 185 445
Cuts at: 6754 325
Size: 1498
Fragments arranged by size:
2196 1612 1498 1255 566 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4014 6173 7885
Size: 274 80 33 51 1443 2037 2159 1712
Cuts at: 7885 96
Size: 138
Fragments arranged by size:
2159 2037 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2057 2089 2249 2281 3965 3997 4447 4479 7887
Size: 32 160 32 1684 32 450 32 3408
Cuts at: 7887 7919 2057
Size: 32 2065
Fragments arranged by size:
3408 2065 1684 450 160 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7927
XbaI T'CTAG_A
Cuts at: 5263 5263
Size: 7927
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4522 4582 4522
Size: 60 7867
XhoI C'TCGA_G
Cuts at: 1943 2327 4285 1943
Size: 384 1958 5585
Fragments arranged by size:
5585 1958 384
XmaI C'CCGG_G
Cuts at: 1870 5252 1870
Size: 3382 4545
XmnI GAAnn'nnTTC
Cuts at: 2678 5120 6056 2678
Size: 2442 936 4549
Fragments arranged by size:
4549 2442 936
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5735 276
Size: 53 83 186 936 4201 2468
Fragments arranged by size:
4201 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BfrBI BsmBI
Bsu36I FspAI KasI NarI NruI NsiI PacI PmlI
PspOMI PsrI SanDI SfoI SrfI SwaI