Restriction map for
pFN21ASDA1093
(Circular) (Six-base) MAPSORT of: pFN21ASDA1093.seq Check: 443 from: 1 to: 9895
pFN21ASDA1093 9895 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 6901 6901
Size: 9895
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7705 278
Size: 53 83 186 936 6169 2468
Fragments arranged by size:
6169 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2598 3458 7238 1937
Size: 661 860 3780 4594
Fragments arranged by size:
4594 3780 860 661
Acc65I G'GTAC_C
Cuts at: 5740 7216 5740
Size: 1476 8419
AclI AA'CG_TT
Cuts at: 1563 4572 6351 8022 8395 1563
Size: 3009 1779 1671 373 3063
Fragments arranged by size:
3063 3009 1779 1671 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2902 4686 5109 5797 6348 7958 9342 9610
Size: 1270 1784 423 688 551 1610 1384 268
Cuts at: 9610 1632
Size: 1917
Fragments arranged by size:
1917 1784 1610 1384 1270 688 551 423
268
AfeI AGC'GCT
Cuts at: 2448 3988 7024 2448
Size: 1540 3036 5319
Fragments arranged by size:
5319 3036 1540
AflII C'TTAA_G
Cuts at: 820 1017 3169 820
Size: 197 2152 7546
Fragments arranged by size:
7546 2152 197
AflIII A'CryG_T
Cuts at: 1203 6801 8794 9771 1203
Size: 5598 1993 977 1327
Fragments arranged by size:
5598 1993 1327 977
AgeI A'CCGG_T
Cuts at: 8726 8726
Size: 9895
AhdI GACnn_n'nnGTC
Cuts at: 8624 8624
Size: 9895
AleI CACnn'nnGTG
Cuts at: 1166 5333 6804 1166
Size: 4167 1471 4257
Fragments arranged by size:
4257 4167 1471
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9895
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3390 3422 3390
Size: 32 9863
AlwNI CAG_nnn'CTG
Cuts at: 2411 3335 5629 6934 7638 9210 2411
Size: 924 2294 1305 704 1572 3096
Fragments arranged by size:
3096 2294 1572 1305 924 704
ApaI G_GGCC'C
Cuts at: 4775 5469 5718 7074 4775
Size: 694 249 1356 7596
Fragments arranged by size:
7596 1356 694 249
ApaLI G'TGCA_C
Cuts at: 3021 5646 7952 9108 3021
Size: 2625 2306 1156 3808
Fragments arranged by size:
3808 2625 2306 1156
ApoI r'AATT_y
Cuts at: 1493 2518 3120 3616 4759 5144 5347 5514 6125
Size: 1025 602 496 1143 385 203 167 611
Cuts at: 6125 7204 7430 7582 9691 1493
Size: 1079 226 152 2109 1697
Fragments arranged by size:
2109 1697 1143 1079 1025 611 602 496
385 226 203 167 152
AseI AT'TA_AT
Cuts at: 160 1032 8449 160
Size: 872 7417 1606
Fragments arranged by size:
7417 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9895
AvaI C'yCGr_G
Cuts at: 1870 1943 2286 2533 4782 5174 5973 7004 7220
Size: 73 343 247 2249 392 799 1031 216
Cuts at: 7220 1870
Size: 4545
Fragments arranged by size:
4545 2249 1031 799 392 343 247 216
73
BamHI G'GATC_C
Cuts at: 7225 7592 7225
Size: 367 9528
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2293 2555 3937 5740 6311
Size: 325 217 627 506 262 1382 1803 571
Cuts at: 6311 7216 8671 618
Size: 905 1455 1842
Fragments arranged by size:
1842 1803 1455 1382 905 627 571 506
325 262 217
BanII G_rGCy'C
Cuts at: 1391 2534 4071 4775 4885 5469 5718 5766 7005
Size: 1143 1537 704 110 584 249 48 1239
Cuts at: 7005 7074 7214 1391
Size: 69 140 4072
Fragments arranged by size:
4072 1537 1239 1143 704 584 249 140
110 69 48
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2070 4242 928
Size: 971 171 2172 6581
Fragments arranged by size:
6581 2172 971 171
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2292 2326 5305 5339 5615 5649 6676
Size: 34 355 34 2979 34 276 34 1027
Cuts at: 6676 6710 8086 8120 1903
Size: 34 1376 34 3678
Fragments arranged by size:
3678 2979 1376 1027 355 276 34 34
34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6164 7787 8997 6164
Size: 1623 1210 7062
Fragments arranged by size:
7062 1623 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9895
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2190 8506 136
Size: 107 122 71 631 605 518 6316 1525
Fragments arranged by size:
6316 1525 631 605 518 122 107 71
BglII A'GATC_T
Cuts at: 3701 9890 3701
Size: 6189 3706
BlpI GC'TnA_GC
Cuts at: 5498 6879 7312 5498
Size: 1381 433 8081
Fragments arranged by size:
8081 1381 433
Bme1580I G_kGCm'C
Cuts at: 1790 2142 3025 4775 5469 5650 5718 6743 7074
Size: 352 883 1750 694 181 68 1025 331
Cuts at: 7074 7956 9112 1790
Size: 882 1156 2573
Fragments arranged by size:
2573 1750 1156 1025 883 882 694 352
331 181 68
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9895
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3288 3429 3576
Size: 416 53 83 399 24 1871 141 147
Cuts at: 3576 4156 4632 5352 5377 5464 5515 6032 7008
Size: 580 476 720 25 87 51 517 976
Cuts at: 7008 7626 8584 442
Size: 618 958 1753
Fragments arranged by size:
1871 1753 976 958 720 618 580 517
476 416 399 147 141 87 83 53
51 25 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9895
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2433 3217 3285 3648 4548 4615 6105 6448
Size: 1194 784 68 363 900 67 1490 343
Cuts at: 6448 6550 6622 6956 8555 1239
Size: 102 72 334 1599 2579
Fragments arranged by size:
2579 1599 1490 1194 900 784 363 343
334 102 72 68 67
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5402 5583 6700 8020 8885 9183 5402
Size: 181 1117 1320 865 298 6114
Fragments arranged by size:
6114 1320 1117 865 298 181
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3544 4707 6932 8558 882
Size: 617 2045 1163 2225 1626 2219
Fragments arranged by size:
2225 2219 2045 1626 1163 617
BsaAI yAC'GTr
Cuts at: 493 1204 7192 7686 493
Size: 711 5988 494 2702
Fragments arranged by size:
5988 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 9889 9889
Size: 9895
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4228 7702 8084 8719
Size: 53 83 186 936 2695 3474 382 635
Cuts at: 8719 275
Size: 1451
Fragments arranged by size:
3474 2695 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 8328 8726 9000 9147 9558 8328
Size: 398 274 147 411 8665
Fragments arranged by size:
8665 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2543 2573 3143 3173 3390
Size: 30 165 30 1140 30 570 30 217
Cuts at: 3390 3420 6595 6625 1178
Size: 30 3175 30 4448
Fragments arranged by size:
4448 3175 1140 570 217 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2385 2533 4167 4919 4922 4932 5469 7185
Size: 1199 148 1634 752 3 10 537 1716
Cuts at: 7185 1186
Size: 3896
Fragments arranged by size:
3896 1716 1634 1199 752 537 148 10
3
BseYI C'CCAG_C
Cuts at: 3888 4017 4031 4199 4432 4774 4851 4892 4896
Size: 129 14 168 233 342 77 41 4
Cuts at: 4896 4974 6205 9098 9594 3888
Size: 78 1231 2893 496 4189
Fragments arranged by size:
4189 2893 1231 496 342 233 168 129
78 77 41 14 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2386 3321 5309 5357 6777 6782 1163
Size: 573 650 935 1988 48 1420 5 4276
Fragments arranged by size:
4276 1988 1420 935 650 573 48 5
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2178 3797 7345 8106 9134 1540
Size: 275 186 177 1619 3548 761 1028 2301
Fragments arranged by size:
3548 2301 1619 1028 761 275 186 177
BsiHKAI G_wGCw'C
Cuts at: 2711 3025 3242 3876 4071 4885 5009 5650 7956
Size: 314 217 634 195 814 124 641 2306
Cuts at: 7956 8041 9112 2711
Size: 85 1071 3494
Fragments arranged by size:
3494 2306 1071 814 641 634 314 217
195 124 85
BsmI GAATG_Cn'
Cuts at: 3207 7406 7499 3207
Size: 4199 93 5603
Fragments arranged by size:
5603 4199 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4439 4439
Size: 9895
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2142 2534 2711 3025 3242 3876 4071
Size: 399 352 392 177 314 217 634 195
Cuts at: 4071 4775 4885 5009 5469 5650 5718 5766 6743
Size: 704 110 124 460 181 68 48 977
Cuts at: 6743 7005 7074 7214 7956 8041 9112 1391
Size: 262 69 140 742 85 1071 2174
Fragments arranged by size:
2174 1071 977 742 704 634 460 399
392 352 314 262 217 195 181 177
140 124 110 85 69 68 48
BspEI T'CCGG_A
Cuts at: 9558 9558
Size: 9895
BspHI T'CATG_A
Cuts at: 5193 7784 9514 5193
Size: 2591 1730 5574
Fragments arranged by size:
5574 2591 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4803 6070 6901 6979 7250 844
Size: 3959 1267 831 78 271 3489
Fragments arranged by size:
3959 3489 1267 831 271 78
BsrBI CCG'CTC
Cuts at: 1394 1823 7782 1394
Size: 429 5959 3507
Fragments arranged by size:
5959 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3682 4390 6112 8390 8564 65
Size: 1183 2434 708 1722 2278 174 1396
Fragments arranged by size:
2434 2278 1722 1396 1183 708 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6941 8539 8726 1700
Size: 110 5131 1598 187 2869
Fragments arranged by size:
5131 2869 1598 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9895
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9895
BssSI C'ACGA_G
Cuts at: 7955 8967 7955
Size: 1012 8883
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 9895
BstBI TT'CG_AA
Cuts at: 2007 8699 2007
Size: 6692 3203
BstEII G'GTnAC_C
Cuts at: 1184 5982 6057 7157 1184
Size: 4798 75 1100 3922
Fragments arranged by size:
4798 3922 1100 75
BstXI CCAn_nnnn'nTGG
Cuts at: 2239 2952 5239 2239
Size: 713 2287 6895
Fragments arranged by size:
6895 2287 713
BstYI r'GATC_y
Cuts at: 1972 3290 3701 4427 6403 6958 7117 7225 7592
Size: 1318 411 726 1976 555 159 108 367
Cuts at: 7592 7977 7994 9446 9532 9544 9890 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1976 1452 1318 726 555 411 385
367 346 159 108 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2811 3948 4623 2811
Size: 1137 675 8083
Fragments arranged by size:
8083 1137 675
BtgI C'CryG_G
Cuts at: 513 1065 3186 6143 6963 513
Size: 552 2121 2957 820 3445
Fragments arranged by size:
3445 2957 2121 820 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2187 5180 487
Size: 683 56 961 2993 5202
Fragments arranged by size:
5202 2993 961 683 56
BtsI GCAGTG_nn'
Cuts at: 2250 4954 5648 6707 6741 6794 7016 7411 8205
Size: 2704 694 1059 34 53 222 395 794
Cuts at: 8205 8225 2250
Size: 20 3920
Fragments arranged by size:
3920 2704 1059 794 694 395 222 53
34 20
ClaI AT'CG_AT
Cuts at: 1554 5206 1554
Size: 3652 6243
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9860
DraI TTT'AAA
Cuts at: 5878 7199 5878
Size: 1321 8574
DraIII CAC_nnn'GTG
Cuts at: 2329 3921 6026 6311 2329
Size: 1592 2105 285 5913
Fragments arranged by size:
5913 2105 1592 285
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4871 6185 8902 809
Size: 507 3555 1314 2717 1802
Fragments arranged by size:
3555 2717 1802 1314 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2175 2954 3787
Size: 54 1425 50 275 18 345 779 833
Cuts at: 3787 6028 6777 6943 7342 8231 9860 8
Size: 2241 749 166 399 889 1629 43
Fragments arranged by size:
2241 1629 1425 889 833 779 749 399
345 275 166 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2175 7342 1537
Size: 275 363 5167 4090
Fragments arranged by size:
5167 4090 363 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2610 2699 3927 4144 4644 4896 7825 1353
Size: 1257 89 1228 217 500 252 2929 3423
Fragments arranged by size:
3423 2929 1257 1228 500 252 217 89
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 8482 8856 9002 387
Size: 1064 138 6893 374 146 1280
Fragments arranged by size:
6893 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4069 4883 4069
Size: 814 9081
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2433 2902 3217 3285 3648 4548 4615
Size: 393 801 469 315 68 363 900 67
Cuts at: 4615 4686 5109 5797 6105 6348 6448 6550 6622
Size: 71 423 688 308 243 100 102 72
Cuts at: 6622 6956 7958 8555 9342 9610 1239
Size: 334 1002 597 787 268 1524
Fragments arranged by size:
1524 1002 900 801 787 688 597 469
423 393 363 334 315 308 268 243
102 100 72 71 68 67
EcoO109I rG'GnC_Cy
Cuts at: 2390 3311 3468 4771 5465 5466 6038 6968 7070
Size: 921 157 1303 694 1 572 930 102
Cuts at: 7070 7071 2390
Size: 1 5214
Fragments arranged by size:
5214 1303 930 921 694 572 157 102
1 1
EcoRI G'AATT_C
Cuts at: 4759 6125 7204 7582 4759
Size: 1366 1079 378 7072
Fragments arranged by size:
7072 1366 1079 378
EcoRV GAT'ATC
Cuts at: 5191 6004 5191
Size: 813 9082
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4413 4413
Size: 9895
FspI TGC'GCA
Cuts at: 8401 8401
Size: 9895
HaeII r_GCGC'y
Cuts at: 1678 2271 2450 3990 5952 7026 7059 9042 1678
Size: 593 179 1540 1962 1074 33 1983 2531
Fragments arranged by size:
2531 1983 1962 1540 1074 593 179 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2633 2665 3317 3349 3941
Size: 32 135 32 1061 32 652 32 592
Cuts at: 3941 3973 4820 4852 5029 5061 5188 5220 5434
Size: 32 847 32 177 32 127 32 214
Cuts at: 5434 5466 6158 6190 6371 6403 6599 6631 6706
Size: 32 692 32 181 32 196 32 75
Cuts at: 6706 6738 8536 8568 8610 8642 1373
Size: 32 1798 32 42 32 2626
Fragments arranged by size:
2626 1798 1061 847 692 652 592 214
196 181 177 135 127 75 42 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 6675 7239 7485 669
Size: 988 281 4737 564 246 3079
Fragments arranged by size:
4737 3079 988 564 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9895
HpaI GTT'AAC
Cuts at: 7485 7485
Size: 9895
KpnI G_GTAC'C
Cuts at: 5744 7220 5744
Size: 1476 8419
MfeI C'AATT_G
Cuts at: 2187 4300 7494 2187
Size: 2113 3194 4588
Fragments arranged by size:
4588 3194 2113
MluI A'CGCG_T
Cuts at: 9771 9771
Size: 9895
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2571 2859 3268 3709 4167 4655 6154 6459
Size: 1266 288 409 441 458 488 1499 305
Cuts at: 6459 7158 9009 9193 1305
Size: 699 1851 184 2007
Fragments arranged by size:
2007 1851 1499 1266 699 488 458 441
409 305 288 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2956 6030 10
Size: 54 1425 343 1124 3074 3875
Fragments arranged by size:
3875 3074 1425 1124 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4805 5333 5943 6804 6806 7853 8212
Size: 648 3639 528 610 861 2 1047 359
Cuts at: 8212 8371 518
Size: 159 2042
Fragments arranged by size:
3639 2042 1047 861 648 610 528 359
159 2
MspA1I CmG'CkG
Cuts at: 1606 4593 4602 4896 5629 6481 6917 7038 7311
Size: 2987 9 294 733 852 436 121 273
Cuts at: 7311 7988 9136 9381 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2987 2120 1148 852 733 677 436 294
273 245 121 9
NcoI C'CATG_G
Cuts at: 513 1065 3186 6143 513
Size: 552 2121 2957 4265
Fragments arranged by size:
4265 2957 2121 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9895
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9895
NotI GC'GGCC_GC
Cuts at: 7342 7342
Size: 9895
NspI r_CATG'y
Cuts at: 1132 1229 2284 2371 5538 6697 6805 7253 8798
Size: 97 1055 87 3167 1159 108 448 1545
Cuts at: 8798 1132
Size: 2229
Fragments arranged by size:
3167 2229 1545 1159 1055 448 108 97
87
PasI CC'CwG_GG
Cuts at: 1348 3867 6736 1348
Size: 2519 2869 4507
Fragments arranged by size:
4507 2869 2519
PciI A'CATG_T
Cuts at: 6801 8794 6801
Size: 1993 7902
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2216 3146 4467 4644 5795 6497 7129 1108
Size: 1108 930 1321 177 1151 702 632 3874
Fragments arranged by size:
3874 1321 1151 1108 930 702 632 177
PfoI T'CCnGG_A
Cuts at: 3233 3389 3470 4466 6464 3233
Size: 156 81 996 1998 6664
Fragments arranged by size:
6664 1998 996 156 81
PmeI GTTT'AAAC
Cuts at: 7199 7199
Size: 9895
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2681 2713 3390 3422 4121 4153 5854 5886 7964
Size: 32 677 32 699 32 1701 32 2078
Cuts at: 7964 7996 9477 9509 2681
Size: 32 1481 32 3067
Fragments arranged by size:
3067 2078 1701 1481 699 677 32 32
32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3468 6038 6968 3468
Size: 2570 930 6395
Fragments arranged by size:
6395 2570 930
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9895
PsiI TTA'TAA
Cuts at: 4728 7465 4728
Size: 2737 7158
PspOMI G'GGCC_C
Cuts at: 4771 5465 5714 7070 4771
Size: 694 249 1356 7596
Fragments arranged by size:
7596 1356 694 249
PstI C_TGCA'G
Cuts at: 830 2457 5655 6898 7247 830
Size: 1627 3198 1243 349 3478
Fragments arranged by size:
3478 3198 1627 1243 349
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9895
PvuII CAG'CTG
Cuts at: 4593 4896 5629 6917 4593
Size: 303 733 1288 7571
Fragments arranged by size:
7571 1288 733 303
SacI G_AGCT'C
Cuts at: 4071 4885 4071
Size: 814 9081
SalI G'TCGA_C
Cuts at: 1936 7237 1936
Size: 5301 4594
SapI GCTCTTCn'nnn_
Cuts at: 2699 4644 2699
Size: 1945 7950
SbfI CC_TGCA'GG
Cuts at: 7247 7247
Size: 9895
ScaI AGT'ACT
Cuts at: 8143 9855 8143
Size: 1712 8183
SexAI A'CCwGG_T
Cuts at: 6307 7153 6307
Size: 846 9049
SfcI C'TryA_G
Cuts at: 826 1045 2453 2844 4080 5121 5651 5913 5952
Size: 219 1408 391 1236 1041 530 262 39
Cuts at: 5952 6322 6894 7243 8378 9059 9250 826
Size: 370 572 349 1135 681 191 1471
Fragments arranged by size:
1471 1408 1236 1135 1041 681 572 530
391 370 349 262 219 191 39
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9895
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9895
SmaI CCC'GGG
Cuts at: 1872 5975 7222 1872
Size: 4103 1247 4545
Fragments arranged by size:
4545 4103 1247
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3169 4782 5417 5598 6679 7999
Size: 197 926 1226 1613 635 181 1081 1320
Cuts at: 7999 8900 9162 820
Size: 901 262 1553
Fragments arranged by size:
1613 1553 1320 1226 1081 926 901 635
262 197 181
SnaBI TAC'GTA
Cuts at: 493 7192 7686 493
Size: 6699 494 2702
Fragments arranged by size:
6699 2702 494
SpeI A'CTAG_T
Cuts at: 152 2729 152
Size: 2577 7318
SphI G_CATG'C
Cuts at: 1132 2371 6697 7253 1132
Size: 1239 4326 556 3774
Fragments arranged by size:
4326 3774 1239 556
SspI AAT'ATT
Cuts at: 5 52 7819 5
Size: 47 7767 2081
Fragments arranged by size:
7767 2081 47
StuI AGG'CCT
Cuts at: 1517 3946 1517
Size: 2429 7466
StyI C'CwwG_G
Cuts at: 513 1065 2374 2488 3186 3670 3941 4765 6143
Size: 552 1309 114 698 484 271 824 1378
Cuts at: 6143 6780 7334 513
Size: 637 554 3074
Fragments arranged by size:
3074 1378 1309 824 698 637 554 552
484 271 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3783 4045 4323 4398 6014 7922 7939
Size: 1255 2203 262 278 75 1616 1908 17
Cuts at: 7939 8092 8277 8722 325
Size: 153 185 445 1498
Fragments arranged by size:
2203 1908 1616 1498 1255 445 278 262
185 153 75 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 8141 9853 96
Size: 274 80 33 51 1443 6164 1712 138
Fragments arranged by size:
6164 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2869 2901 3905 3937 4199 4231 6595 6627 9855
Size: 32 1004 32 262 32 2364 32 3228
Cuts at: 9855 9887 2869
Size: 32 2877
Fragments arranged by size:
3228 2877 2364 1004 262 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3329 3568 1316
Size: 2013 239 7643
Fragments arranged by size:
7643 2013 239
XbaI T'CTAG_A
Cuts at: 7231 7231
Size: 9895
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2577 5725 6141 6525 6921 2577
Size: 3148 416 384 396 5551
Fragments arranged by size:
5551 3148 416 396 384
XhoI C'TCGA_G
Cuts at: 1943 4782 1943
Size: 2839 7056
XmaI C'CCGG_G
Cuts at: 1870 5973 7220 1870
Size: 4103 1247 4545
Fragments arranged by size:
4545 4103 1247
XmnI GAAnn'nnTTC
Cuts at: 3703 5131 5593 8024 3703
Size: 1428 462 2431 5574
Fragments arranged by size:
5574 2431 1428 462
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7703 276
Size: 53 83 186 936 6169 2468
Fragments arranged by size:
6169 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I
BsiWI BstZ17I EcoNI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII
SacII SanDI SfiI SfoI SrfI SwaI