Restriction map for
pFN21ASDA1089
(Circular) (Six-base) MAPSORT of: pFN21ASDA1089.seq Check: 6012 from: 1 to: 7777
pFN21ASDA1089 7777 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4895 4895
Size: 7777
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5587 278
Size: 53 83 186 936 4051 2468
Fragments arranged by size:
4051 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2280 3858 5120 1937
Size: 343 1578 1262 4594
Fragments arranged by size:
4594 1578 1262 343
Acc65I G'GTAC_C
Cuts at: 4743 5098 4743
Size: 355 7422
AclI AA'CG_TT
Cuts at: 1563 5904 6277 1563
Size: 4341 373 3063
Fragments arranged by size:
4341 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2840 3852 3944 4505 4727 4847 5840 7224
Size: 1208 1012 92 561 222 120 993 1384
Cuts at: 7224 7492 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1208 1012 993 561 268 222
120 92
AfeI AGC'GCT
Cuts at: 3758 3758
Size: 7777
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7580
AflIII A'CryG_T
Cuts at: 1203 6676 7653 1203
Size: 5473 977 1327
Fragments arranged by size:
5473 1327 977
AgeI A'CCGG_T
Cuts at: 6608 6608
Size: 7777
AhdI GACnn_n'nnGTC
Cuts at: 6506 6506
Size: 7777
AleI CACnn'nnGTG
Cuts at: 1166 5016 1166
Size: 3850 3927
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2385 3171 4388 5022 1184
Size: 1201 786 1217 634 3939
Fragments arranged by size:
3939 1217 1201 786 634
AlwNI CAG_nnn'CTG
Cuts at: 2495 3326 3881 3967 5520 7092 2495
Size: 831 555 86 1553 1572 3180
Fragments arranged by size:
3180 1572 1553 831 555 86
ApaI G_GGCC'C
Cuts at: 3695 4729 3695
Size: 1034 6743
ApaLI G'TGCA_C
Cuts at: 3119 5834 6990 3119
Size: 2715 1156 3906
Fragments arranged by size:
3906 2715 1156
ApoI r'AATT_y
Cuts at: 1493 5086 5312 5464 7573 1493
Size: 3593 226 152 2109 1697
Fragments arranged by size:
3593 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6331 160
Size: 872 5299 1606
Fragments arranged by size:
5299 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7777
AvaI C'yCGr_G
Cuts at: 1870 1943 3483 3648 4728 5102 1870
Size: 73 1540 165 1080 374 4545
Fragments arranged by size:
4545 1540 1080 374 165 73
BamHI G'GATC_C
Cuts at: 3140 4915 5107 5474 3140
Size: 1775 192 367 5443
Fragments arranged by size:
5443 1775 367 192
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2678 4743 5098 6553 618
Size: 325 217 627 891 2065 355 1455 1842
Fragments arranged by size:
2065 1842 1455 891 627 355 325 217
BanII G_rGCy'C
Cuts at: 1391 2061 2208 3500 3645 3695 3893 4058 4729
Size: 670 147 1292 145 50 198 165 671
Cuts at: 4729 5096 1391
Size: 367 4072
Fragments arranged by size:
4072 1292 671 670 367 198 165 147
145 50
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3994 928
Size: 971 2095 4711
Fragments arranged by size:
4711 2095 971
BbvCI CC'TCA_GC
Cuts at: 2256 3020 2256
Size: 764 7013
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5968 6002 1903
Size: 34 4031 34 3678
Fragments arranged by size:
4031 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5669 6879 5669
Size: 1210 6567
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7777
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3690 4362 4901
Size: 107 122 71 631 605 2018 672 539
Cuts at: 4901 5000 6388 136
Size: 99 1388 1525
Fragments arranged by size:
2018 1525 1388 672 631 605 539 122
107 99 71
BglII A'GATC_T
Cuts at: 4156 7772 4156
Size: 3616 4161
BlpI GC'TnA_GC
Cuts at: 3225 4289 4509 5194 3225
Size: 1064 220 685 5808
Fragments arranged by size:
5808 1064 685 220
Bme1580I G_kGCm'C
Cuts at: 1790 2524 2681 3123 3695 4729 5838 6994 1790
Size: 734 157 442 572 1034 1109 1156 2573
Fragments arranged by size:
2573 1156 1109 1034 734 572 442 157
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7777
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2228 3690 3698
Size: 416 53 83 399 24 811 1462 8
Cuts at: 3698 4408 4564 5508 6466 442
Size: 710 156 944 958 1753
Fragments arranged by size:
1753 1462 958 944 811 710 416 399
156 83 53 24 8
BmtI G_CTAG'C
Cuts at: 1056 2612 3708 1056
Size: 1556 1096 5125
Fragments arranged by size:
5125 1556 1096
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2968 3895 3907 2968
Size: 927 12 6838
Fragments arranged by size:
6838 927 12
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2993 3659 3782 4103 4562 4769 6437 1239
Size: 1754 666 123 321 459 207 1668 2579
Fragments arranged by size:
2579 1754 1668 666 459 321 207 123
Bpu10I CC'TnA_GC
Cuts at: 2256 3020 3327 2256
Size: 764 307 6706
Fragments arranged by size:
6706 764 307
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2762 3451 5902 6767 7065 2762
Size: 689 2451 865 298 3474
Fragments arranged by size:
3474 2451 865 689 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6440 882
Size: 617 4941 2219
Fragments arranged by size:
4941 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5074 5568 493
Size: 711 3870 494 2702
Fragments arranged by size:
3870 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 7771 7771
Size: 7777
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5584 5966 6601 275
Size: 53 83 186 936 4051 382 635 1451
Fragments arranged by size:
4051 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6210 6608 6882 7029 7440 6210
Size: 398 274 147 411 6547
Fragments arranged by size:
6547 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2252 2282 2990 3020 4742
Size: 30 165 30 849 30 708 30 1722
Cuts at: 4742 4772 1178
Size: 30 4183
Fragments arranged by size:
4183 1722 849 708 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2273 2637 2998 3056 3059 3324 3326 3329
Size: 1087 364 361 58 3 265 2 3
Cuts at: 3329 3629 3785 3899 4316 4925 1186
Size: 300 156 114 417 609 4038
Fragments arranged by size:
4038 1087 609 417 364 361 300 265
156 114 58 3 3 2
BseYI C'CCAG_C
Cuts at: 2674 4964 6980 7476 2674
Size: 2290 2016 496 2975
Fragments arranged by size:
2975 2290 2016 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2213 3177 1163
Size: 573 477 964 5763
Fragments arranged by size:
5763 964 573 477
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5227 5988 7016 1540
Size: 275 186 3226 761 1028 2301
Fragments arranged by size:
3226 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2208 2339 3123 4058 5838 5923 6994 2208
Size: 131 784 935 1780 85 1071 2991
Fragments arranged by size:
2991 1780 1071 935 784 131 85
BsmI GAATG_Cn'
Cuts at: 2664 3257 5288 5381 2664
Size: 593 2031 93 5060
Fragments arranged by size:
5060 2031 593 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2061 2208 2339 2524 2681 3123 3500
Size: 399 271 147 131 185 157 442 377
Cuts at: 3500 3645 3695 3893 4058 4729 5096 5838 5923
Size: 145 50 198 165 671 367 742 85
Cuts at: 5923 6994 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 671 442 399 377 367
271 198 185 165 157 147 145 131
85 50
BspEI T'CCGG_A
Cuts at: 7440 7440
Size: 7777
BspHI T'CATG_A
Cuts at: 4494 5666 7396 4494
Size: 1172 1730 4875
Fragments arranged by size:
4875 1730 1172
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2736 3009 3798 4239 4895 5132 844
Size: 1892 273 789 441 656 237 3489
Fragments arranged by size:
3489 1892 789 656 441 273 237
BsrBI CCG'CTC
Cuts at: 1394 1823 4240 4816 5664 1394
Size: 429 2417 576 848 3507
Fragments arranged by size:
3507 2417 848 576 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3162 3708 4407 6272 6446 65
Size: 1183 1914 546 699 1865 174 1396
Fragments arranged by size:
1914 1865 1396 1183 699 546 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6421 6608 1700
Size: 110 4611 187 2869
Fragments arranged by size:
4611 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7777
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7777
BssSI C'ACGA_G
Cuts at: 5837 6849 5837
Size: 1012 6765
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3164 4323 794
Size: 2370 1159 4248
Fragments arranged by size:
4248 2370 1159
BstBI TT'CG_AA
Cuts at: 2007 6581 2007
Size: 4574 3203
BstEII G'GTnAC_C
Cuts at: 1184 3627 4930 1184
Size: 2443 1303 4031
Fragments arranged by size:
4031 2443 1303
BstXI CCAn_nnnn'nTGG
Cuts at: 3269 4127 3269
Size: 858 6919
BstYI r'GATC_y
Cuts at: 1972 3140 3322 4156 4863 4915 5107 5474 5859
Size: 1168 182 834 707 52 192 367 385
Cuts at: 5859 5876 7328 7414 7426 7772 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1168 834 707 385 367 346
192 182 86 52 17 12
BstZ17I GTA'TAC
Cuts at: 2281 2281
Size: 7777
Bsu36I CC'TnA_GG
Cuts at: 2186 2500 4146 2186
Size: 314 1646 5817
Fragments arranged by size:
5817 1646 314
BtgI C'CryG_G
Cuts at: 513 1065 2934 513
Size: 552 1869 5356
Fragments arranged by size:
5356 1869 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2875 4333 487
Size: 683 56 1649 1458 3931
Fragments arranged by size:
3931 1649 1458 683 56
BtsI GCAGTG_nn'
Cuts at: 2441 5060 5293 6087 6107 2441
Size: 2619 233 794 20 4111
Fragments arranged by size:
4111 2619 794 233 20
ClaI AT'CG_AT
Cuts at: 1554 4689 1554
Size: 3135 4642
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3821 3856 4049 4084 529
Size: 35 3257 35 193 35 4222
Fragments arranged by size:
4222 3257 193 35 35 35
DraI TTT'AAA
Cuts at: 4762 5081 4762
Size: 319 7458
DraIII CAC_nnn'GTG
Cuts at: 2236 2236
Size: 7777
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3927 6784 809
Size: 507 2611 2857 1802
Fragments arranged by size:
2857 2611 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3027 3825 3972
Size: 54 1425 50 275 18 1197 798 147
Cuts at: 3972 4644 4654 5224 6113 7742 8
Size: 672 10 570 889 1629 43
Fragments arranged by size:
1629 1425 1197 889 798 672 570 275
147 54 50 43 18 10
EagI C'GGCC_G
Cuts at: 1537 1812 5224 1537
Size: 275 3412 4090
Fragments arranged by size:
4090 3412 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2999 3228 3606 4227 4823 5707 1353
Size: 1646 229 378 621 596 884 3423
Fragments arranged by size:
3423 1646 884 621 596 378 229
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3389 4625 6364 6738 6884 387
Size: 1064 138 1800 1236 1739 374 146 1280
Fragments arranged by size:
1800 1739 1280 1236 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2206 4056 2206
Size: 1850 5927
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2840 2993 3659 3782 3852 3944 4103
Size: 393 1208 153 666 123 70 92 159
Cuts at: 4103 4505 4562 4727 4769 4847 5840 6437 7224
Size: 402 57 165 42 78 993 597 787
Cuts at: 7224 7492 1239
Size: 268 1524
Fragments arranged by size:
1524 1208 993 787 666 597 402 393
268 165 159 153 123 92 78 70
57 42
EcoO109I rG'GnC_Cy
Cuts at: 2033 3692 3964 4609 4725 4979 2033
Size: 1659 272 645 116 254 4831
Fragments arranged by size:
4831 1659 645 272 254 116
EcoRI G'AATT_C
Cuts at: 5086 5464 5086
Size: 378 7399
EcoRV GAT'ATC
Cuts at: 2573 2573
Size: 7777
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3778 4001 4178 3778
Size: 223 177 7377
Fragments arranged by size:
7377 223 177
FspI TGC'GCA
Cuts at: 4050 4316 6283 4050
Size: 266 1967 5544
Fragments arranged by size:
5544 1967 266
FspAI rTGC'GCAy
Cuts at: 4316 4316
Size: 7777
HaeII r_GCGC'y
Cuts at: 1678 3760 6924 1678
Size: 2082 3164 2531
Fragments arranged by size:
3164 2531 2082
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2112 2144 2518 2550 3494
Size: 32 135 32 540 32 374 32 944
Cuts at: 3494 3526 3572 3604 4175 4207 4509 4541 6418
Size: 32 46 32 571 32 302 32 1877
Cuts at: 6418 6450 6492 6524 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1877 944 571 540 374 302 135
46 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3208 5121 5367 669
Size: 988 281 1270 1913 246 3079
Fragments arranged by size:
3079 1913 1270 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2216 3956 748
Size: 1468 1740 4569
Fragments arranged by size:
4569 1740 1468
HpaI GTT'AAC
Cuts at: 5367 5367
Size: 7777
KpnI G_GTAC'C
Cuts at: 4747 5102 4747
Size: 355 7422
MfeI C'AATT_G
Cuts at: 5376 5376
Size: 7777
MluI A'CGCG_T
Cuts at: 7653 7653
Size: 7777
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3790 3879 3911 4463 6891 7075 1305
Size: 2485 89 32 552 2428 184 2007
Fragments arranged by size:
2485 2428 2007 552 184 89 32
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3029 4646 10
Size: 54 1425 343 1197 1617 3141
Fragments arranged by size:
3141 1617 1425 1197 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3740 4040 5016 5735 6094 6253 518
Size: 648 2574 300 976 719 359 159 2042
Fragments arranged by size:
2574 2042 976 719 648 359 300 159
MspA1I CmG'CkG
Cuts at: 1606 2425 2674 4540 4875 4885 5006 5193 5870
Size: 819 249 1866 335 10 121 187 677
Cuts at: 5870 7018 7263 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1866 1148 819 677 335 249 245
187 121 10
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7225
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7777
NheI G'CTAG_C
Cuts at: 1052 2608 3704 1052
Size: 1556 1096 5125
Fragments arranged by size:
5125 1556 1096
NotI GC'GGCC_GC
Cuts at: 5224 5224
Size: 7777
NspI r_CATG'y
Cuts at: 1132 1229 2535 5135 6680 1132
Size: 97 1306 2600 1545 2229
Fragments arranged by size:
2600 2229 1545 1306 97
PasI CC'CwG_GG
Cuts at: 1348 4721 4897 1348
Size: 3373 176 4228
Fragments arranged by size:
4228 3373 176
PciI A'CATG_T
Cuts at: 6676 6676
Size: 7777
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4391 1108
Size: 3283 4494
PfoI T'CCnGG_A
Cuts at: 2970 3072 3948 4005 4119 2970
Size: 102 876 57 114 6628
Fragments arranged by size:
6628 876 114 102 57
PmeI GTTT'AAAC
Cuts at: 5081 5081
Size: 7777
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5846 5878 7359 7391 5846
Size: 32 1481 32 6232
Fragments arranged by size:
6232 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3964 3964
Size: 7777
PshAI GACnn'nnGTC
Cuts at: 1613 2456 3519 1613
Size: 843 1063 5871
Fragments arranged by size:
5871 1063 843
PsiI TTA'TAA
Cuts at: 5347 5347
Size: 7777
PspOMI G'GGCC_C
Cuts at: 3691 4725 3691
Size: 1034 6743
PstI C_TGCA'G
Cuts at: 830 2436 3222 4377 4440 4557 5001 5129 830
Size: 1606 786 1155 63 117 444 128 3478
Fragments arranged by size:
3478 1606 1155 786 444 128 117 63
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7777
PvuII CAG'CTG
Cuts at: 2425 2674 4540 4875 4885 2425
Size: 249 1866 335 10 5317
Fragments arranged by size:
5317 1866 335 249 10
SacI G_AGCT'C
Cuts at: 2208 4058 2208
Size: 1850 5927
SalI G'TCGA_C
Cuts at: 1936 5119 1936
Size: 3183 4594
SapI GCTCTTCn'nnn_
Cuts at: 4227 4227
Size: 7777
SbfI CC_TGCA'GG
Cuts at: 5001 5129 5001
Size: 128 7649
ScaI AGT'ACT
Cuts at: 2699 6025 7737 2699
Size: 3326 1712 2739
Fragments arranged by size:
3326 2739 1712
SexAI A'CCwGG_T
Cuts at: 2364 2364
Size: 7777
SfcI C'TryA_G
Cuts at: 826 1045 2353 2432 2915 3218 4373 4436 4553
Size: 219 1308 79 483 303 1155 63 117
Cuts at: 4553 4997 5125 6260 6941 7132 826
Size: 444 128 1135 681 191 1471
Fragments arranged by size:
1471 1308 1155 1135 681 483 444 303
219 191 128 117 79 63
SfiI GGCCn_nnn'nGGCC
Cuts at: 3690 3690
Size: 7777
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7777
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7777
SmaI CCC'GGG
Cuts at: 1872 3485 4730 5104 1872
Size: 1613 1245 374 4545
Fragments arranged by size:
4545 1613 1245 374
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2777 3466 5881 6782 7044 820
Size: 197 926 834 689 2415 901 262 1553
Fragments arranged by size:
2415 1553 926 901 834 689 262 197
SnaBI TAC'GTA
Cuts at: 493 5074 5568 493
Size: 4581 494 2702
Fragments arranged by size:
4581 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7777
SphI G_CATG'C
Cuts at: 1132 5135 1132
Size: 4003 3774
SrfI GCCC'GGGC
Cuts at: 3485 3485
Size: 7777
SspI AAT'ATT
Cuts at: 5 52 5701 5
Size: 47 5649 2081
Fragments arranged by size:
5649 2081 47
StuI AGG'CCT
Cuts at: 1517 3838 4947 1517
Size: 2321 1109 4347
Fragments arranged by size:
4347 2321 1109
StyI C'CwwG_G
Cuts at: 513 1065 2029 3291 4563 4676 5216 513
Size: 552 964 1262 1272 113 540 3074
Fragments arranged by size:
3074 1272 1262 964 552 540 113
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2247 3028 3344 3429 3433 3752 4619
Size: 1255 667 781 316 85 4 319 867
Cuts at: 4619 4734 5804 5821 5974 6159 6604 325
Size: 115 1070 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1070 867 781 667 445 319
316 185 153 115 85 17 4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2697 3150 6023
Size: 274 80 33 51 1443 720 453 2873
Cuts at: 6023 7735 96
Size: 1712 138
Fragments arranged by size:
2873 1712 1443 720 453 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2252 2284 2420 2452 4933 4965 7737 7769 2252
Size: 32 136 32 2481 32 2772 32 2260
Fragments arranged by size:
2772 2481 2260 136 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3625 1316
Size: 2309 5468
XbaI T'CTAG_A
Cuts at: 5113 5113
Size: 7777
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3022 3298 3022
Size: 276 7501
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7777
XmaI C'CCGG_G
Cuts at: 1870 3483 4728 5102 1870
Size: 1613 1245 374 4545
Fragments arranged by size:
4545 1613 1245 374
XmnI GAAnn'nnTTC
Cuts at: 4001 5906 4001
Size: 1905 5872
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5585 276
Size: 53 83 186 936 4051 2468
Fragments arranged by size:
4051 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI
EcoNI FseI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspXI PsrI RsrII SacII SanDI SfoI
SwaI