Restriction map for
pFN21ASDA1083
(Circular) (Six-base) MAPSORT of: pFN21ASDA1083.seq Check: 1187 from: 1 to: 6481
pFN21ASDA1083 6481 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2326 2326
Size: 6481
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4291 278
Size: 53 83 186 936 2755 2468
Fragments arranged by size:
2755 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3301 3824 1937
Size: 1364 523 4594
Fragments arranged by size:
4594 1364 523
Acc65I G'GTAC_C
Cuts at: 3802 3802
Size: 6481
AclI AA'CG_TT
Cuts at: 1563 4608 4981 1563
Size: 3045 373 3063
Fragments arranged by size:
3063 3045 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4544 5928 6196 1632
Size: 2912 1384 268 1917
Fragments arranged by size:
2912 1917 1384 268
AfeI AGC'GCT
Cuts at: 2264 2264
Size: 6481
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6284
AflIII A'CryG_T
Cuts at: 1203 5380 6357 1203
Size: 4177 977 1327
Fragments arranged by size:
4177 1327 977
AgeI A'CCGG_T
Cuts at: 5312 5312
Size: 6481
AhdI GACnn_n'nnGTC
Cuts at: 5210 5210
Size: 6481
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6481
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2096 1184
Size: 912 5569
AlwNI CAG_nnn'CTG
Cuts at: 2318 3113 4224 5796 2318
Size: 795 1111 1572 3003
Fragments arranged by size:
3003 1572 1111 795
ApaLI G'TGCA_C
Cuts at: 4538 5694 4538
Size: 1156 5325
ApoI r'AATT_y
Cuts at: 1493 2026 2872 2945 3317 3329 3790 4016 4168
Size: 533 846 73 372 12 461 226 152
Cuts at: 4168 6277 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 846 533 461 372 226 152
73 12
AseI AT'TA_AT
Cuts at: 160 1032 5035 160
Size: 872 4003 1606
Fragments arranged by size:
4003 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6481
AvaI C'yCGr_G
Cuts at: 1870 1943 2147 3216 3806 1870
Size: 73 204 1069 590 4545
Fragments arranged by size:
4545 1069 590 204 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3756 3789 3756
Size: 33 6448
BamHI G'GATC_C
Cuts at: 3811 4178 3811
Size: 367 6114
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2066 2080 2338 2344 3802
Size: 325 217 627 279 14 258 6 1458
Cuts at: 3802 5257 618
Size: 1455 1842
Fragments arranged by size:
1842 1458 1455 627 325 279 258 217
14 6
BanII G_rGCy'C
Cuts at: 1391 2241 2300 3046 3202 3800 1391
Size: 850 59 746 156 598 4072
Fragments arranged by size:
4072 850 746 598 156 59
BbeI G_GCGC'C
Cuts at: 2070 2342 2070
Size: 272 6209
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2368 3095 928
Size: 971 469 727 4314
Fragments arranged by size:
4314 971 727 469
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2304 2338 4672 4706 1903
Size: 34 367 34 2334 34 3678
Fragments arranged by size:
3678 2334 367 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3548 4373 5583 3548
Size: 825 1210 4446
Fragments arranged by size:
4446 1210 825
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6481
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2089 2135 2282
Size: 107 122 71 631 605 417 46 147
Cuts at: 2282 2709 5092 136
Size: 427 2383 1525
Fragments arranged by size:
2383 1525 631 605 427 417 147 122
107 71 46
BglII A'GATC_T
Cuts at: 6476 6476
Size: 6481
BlpI GC'TnA_GC
Cuts at: 3898 3898
Size: 6481
Bme1580I G_kGCm'C
Cuts at: 1790 4542 5698 1790
Size: 2752 1156 2573
Fragments arranged by size:
2752 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6481
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3608 4212 5170
Size: 416 53 83 399 24 2191 604 958
Cuts at: 5170 442
Size: 1753
Fragments arranged by size:
2191 1753 958 604 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6481
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2419 2419
Size: 6481
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2775 3372 5141 1239
Size: 1536 597 1769 2579
Fragments arranged by size:
2579 1769 1536 597
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4606 5471 5769 4606
Size: 865 298 5318
Fragments arranged by size:
5318 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5144 882
Size: 617 3645 2219
Fragments arranged by size:
3645 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3172 3778 4272 493
Size: 711 1968 606 494 2702
Fragments arranged by size:
2702 1968 711 606 494
BsaBI GATnn'nnATC
Cuts at: 6475 6475
Size: 6481
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2067 2339 2548 3304
Size: 53 83 186 936 534 272 209 756
Cuts at: 3304 4288 4670 5305 275
Size: 984 382 635 1451
Fragments arranged by size:
1451 984 936 756 635 534 382 272
209 186 83 53
BsaWI w'CCGG_w
Cuts at: 4914 5312 5586 5733 6144 4914
Size: 398 274 147 411 5251
Fragments arranged by size:
5251 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2165 2195 1178
Size: 30 165 30 762 30 5464
Fragments arranged by size:
5464 762 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2231 2308 3473 1186
Size: 1045 77 1165 4194
Fragments arranged by size:
4194 1165 1045 77
BseYI C'CCAG_C
Cuts at: 5684 6180 5684
Size: 496 5985
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5908
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3931 4692 5720 1540
Size: 275 186 1930 761 1028 2301
Fragments arranged by size:
2301 1930 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2300 2725 3046 3291 4542 4627 5698 2300
Size: 425 321 245 1251 85 1071 3083
Fragments arranged by size:
3083 1251 1071 425 321 245 85
BsmI GAATG_Cn'
Cuts at: 3130 3992 4085 3130
Size: 862 93 5526
Fragments arranged by size:
5526 862 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2241 2300 2725 3046 3202 3291 3800
Size: 399 451 59 425 321 156 89 509
Cuts at: 3800 4542 4627 5698 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 509 451 425 399 321
156 89 85 59
BspEI T'CCGG_A
Cuts at: 6144 6144
Size: 6481
BspHI T'CATG_A
Cuts at: 4370 6100 4370
Size: 1730 4751
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2326 3836 844
Size: 1482 1510 3489
Fragments arranged by size:
3489 1510 1482
BsrBI CCG'CTC
Cuts at: 1394 1823 4368 1394
Size: 429 2545 3507
Fragments arranged by size:
3507 2545 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2406 2984 4976 5150 65
Size: 1183 1158 578 1992 174 1396
Fragments arranged by size:
1992 1396 1183 1158 578 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2134 2341 5125 5312 1700
Size: 110 324 207 2784 187 2869
Fragments arranged by size:
2869 2784 324 207 187 110
BsrGI T'GTAC_A
Cuts at: 96 3340 96
Size: 3244 3237
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6481
BssSI C'ACGA_G
Cuts at: 4541 5553 4541
Size: 1012 5469
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3113 3536 3590 794
Size: 2319 423 54 3685
Fragments arranged by size:
3685 2319 423 54
BstBI TT'CG_AA
Cuts at: 2007 5285 2007
Size: 3278 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6481
BstYI r'GATC_y
Cuts at: 1972 2693 2768 3811 4178 4563 4580 6032 6118
Size: 721 75 1043 367 385 17 1452 86
Cuts at: 6118 6130 6476 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1043 721 385 367 346 86
75 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2745 513
Size: 552 1680 4249
Fragments arranged by size:
4249 1680 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5742
Fragments arranged by size:
5742 683 56
BtsI GCAGTG_nn'
Cuts at: 2660 3997 4791 4811 2660
Size: 1337 794 20 4330
Fragments arranged by size:
4330 1337 794 20
ClaI AT'CG_AT
Cuts at: 1554 2421 1554
Size: 867 5614
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3153 3188 529
Size: 35 2589 35 3822
Fragments arranged by size:
3822 2589 35 35
DraI TTT'AAA
Cuts at: 3785 3785
Size: 6481
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5488 809
Size: 507 4172 1802
Fragments arranged by size:
4172 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2583 3928 4817
Size: 54 1425 50 275 18 753 1345 889
Cuts at: 4817 6446 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1345 889 753 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3928 1537
Size: 275 2116 4090
Fragments arranged by size:
4090 2116 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2249 3271 4411 1353
Size: 896 1022 1140 3423
Fragments arranged by size:
3423 1140 1022 896
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2085 2133 5068 5442 5588 387
Size: 1064 138 496 48 2935 374 146 1280
Fragments arranged by size:
2935 1280 1064 496 374 146 138 48
EcoICRI GAG'CTC
Cuts at: 2298 3044 2298
Size: 746 5735
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2775 3372 4544 5141 5928 6196 1239
Size: 393 1143 597 1172 597 787 268 1524
Fragments arranged by size:
1524 1172 1143 787 597 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3051 3051
Size: 6481
EcoO109I rG'GnC_Cy
Cuts at: 3602 3602
Size: 6481
EcoRI G'AATT_C
Cuts at: 2026 3790 4168 2026
Size: 1764 378 4339
Fragments arranged by size:
4339 1764 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2611 3177 2611
Size: 566 5915
FseI GG_CCGG'CC
Cuts at: 2138 2138
Size: 6481
FspI TGC'GCA
Cuts at: 2417 4987 2417
Size: 2570 3911
HaeII r_GCGC'y
Cuts at: 1678 2070 2266 2342 5628 1678
Size: 392 196 76 3286 2531
Fragments arranged by size:
3286 2531 392 196 76
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3542 3574 5122 5154 5196
Size: 32 135 32 1970 32 1548 32 42
Cuts at: 5196 5228 1373
Size: 32 2626
Fragments arranged by size:
2626 1970 1548 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3825 4071 669
Size: 988 281 1887 246 3079
Fragments arranged by size:
3079 1887 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6481
HpaI GTT'AAC
Cuts at: 4071 4071
Size: 6481
KasI G'GCGC_C
Cuts at: 2066 2338 2066
Size: 272 6209
KpnI G_GTAC'C
Cuts at: 3806 3806
Size: 6481
MfeI C'AATT_G
Cuts at: 4080 4080
Size: 6481
MluI A'CGCG_T
Cuts at: 6357 6357
Size: 6481
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3725 5595 5779 1305
Size: 2420 1870 184 2007
Fragments arranged by size:
2420 2007 1870 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2585 10
Size: 54 1425 343 753 3906
Fragments arranged by size:
3906 1425 753 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4439 4798 4957 518
Size: 648 3273 359 159 2042
Fragments arranged by size:
3273 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2191 2939 3897 4574 5722 5967 1606
Size: 585 748 958 677 1148 245 2120
Fragments arranged by size:
2120 1148 958 748 677 585 245
NaeI GCC'GGC
Cuts at: 2136 2136
Size: 6481
NarI GG'CG_CC
Cuts at: 2067 2339 2067
Size: 272 6209
NcoI C'CATG_G
Cuts at: 513 1065 2745 513
Size: 552 1680 4249
Fragments arranged by size:
4249 1680 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6481
NgoMIV G'CCGG_C
Cuts at: 2134 2134
Size: 6481
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6481
NotI GC'GGCC_GC
Cuts at: 3928 3928
Size: 6481
NspI r_CATG'y
Cuts at: 1132 1229 3839 5384 1132
Size: 97 2610 1545 2229
Fragments arranged by size:
2610 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6481
PciI A'CATG_T
Cuts at: 5380 5380
Size: 6481
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6481
PmeI GTTT'AAAC
Cuts at: 3785 3785
Size: 6481
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4550 4582 6063 6095 4550
Size: 32 1481 32 4936
Fragments arranged by size:
4936 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3602 3602
Size: 6481
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6481
PsiI TTA'TAA
Cuts at: 2909 4051 2909
Size: 1142 5339
PstI C_TGCA'G
Cuts at: 830 2701 3118 3833 830
Size: 1871 417 715 3478
Fragments arranged by size:
3478 1871 715 417
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6481
PvuII CAG'CTG
Cuts at: 2939 2939
Size: 6481
SacI G_AGCT'C
Cuts at: 2300 3046 2300
Size: 746 5735
SalI G'TCGA_C
Cuts at: 1936 3823 1936
Size: 1887 4594
SbfI CC_TGCA'GG
Cuts at: 3833 3833
Size: 6481
ScaI AGT'ACT
Cuts at: 3366 4729 6441 3366
Size: 1363 1712 3406
Fragments arranged by size:
3406 1712 1363
SfcI C'TryA_G
Cuts at: 826 1045 2697 3114 3829 4964 5645 5836 826
Size: 219 1652 417 715 1135 681 191 1471
Fragments arranged by size:
1652 1471 1135 715 681 417 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2709 2709
Size: 6481
SfoI GGC'GCC
Cuts at: 2068 2340 2068
Size: 272 6209
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6481
SgrAI Cr'CCGG_yG
Cuts at: 1700 2341 1700
Size: 641 5840
SmaI CCC'GGG
Cuts at: 1872 3808 1872
Size: 1936 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3216 4585 5486 5748 820
Size: 197 926 1273 1369 901 262 1553
Fragments arranged by size:
1553 1369 1273 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3778 4272 493
Size: 3285 494 2702
Fragments arranged by size:
3285 2702 494
SpeI A'CTAG_T
Cuts at: 152 2651 152
Size: 2499 3982
SphI G_CATG'C
Cuts at: 1132 3839 1132
Size: 2707 3774
SspI AAT'ATT
Cuts at: 5 52 3664 4405 5
Size: 47 3612 741 2081
Fragments arranged by size:
3612 2081 741 47
StuI AGG'CCT
Cuts at: 1517 2092 2390 2703 3019 1517
Size: 575 298 313 316 4979
Fragments arranged by size:
4979 575 316 313 298
StyI C'CwwG_G
Cuts at: 513 1065 2586 2745 3920 513
Size: 552 1521 159 1175 3074
Fragments arranged by size:
3074 1521 1175 552 159
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3365 4508 4525 4678 4863 5308 325
Size: 1255 1785 1143 17 153 185 445 1498
Fragments arranged by size:
1785 1498 1255 1143 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2172 2397 3340
Size: 274 80 33 51 1443 195 225 943
Cuts at: 3340 3364 4727 6439 96
Size: 24 1363 1712 138
Fragments arranged by size:
1712 1443 1363 943 274 225 195 138
80 51 33 24
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6441 6473 6441
Size: 32 6449
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6481
XbaI T'CTAG_A
Cuts at: 2605 3817 2605
Size: 1212 5269
XhoI C'TCGA_G
Cuts at: 1943 3216 1943
Size: 1273 5208
XmaI C'CCGG_G
Cuts at: 1870 3806 1870
Size: 1936 4545
XmnI GAAnn'nnTTC
Cuts at: 4610 4610
Size: 6481
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4289 276
Size: 53 83 186 936 2755 2468
Fragments arranged by size:
2755 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BbvCI BfrBI Bpu10I BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FspAI NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SexAI SrfI SwaI XcmI