Restriction map for
pFN21ASDA1075
(Circular) (Six-base) MAPSORT of: pFN21ASDA1075.seq Check: 4608 from: 1 to: 8956
pFN21ASDA1075 8956 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2502 3194 5194 5265 2502
Size: 692 2000 71 6193
Fragments arranged by size:
6193 2000 692 71
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6766 278
Size: 53 83 186 936 5230 2468
Fragments arranged by size:
5230 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3024 3348 6299 1937
Size: 1087 324 2951 4594
Fragments arranged by size:
4594 2951 1087 324
Acc65I G'GTAC_C
Cuts at: 4368 4590 6277 4368
Size: 222 1687 7047
Fragments arranged by size:
7047 1687 222
AclI AA'CG_TT
Cuts at: 1563 7083 7456 1563
Size: 5520 373 3063
Fragments arranged by size:
5520 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2235 2362 2866 4307 5504 7019 8403 8671
Size: 603 127 504 1441 1197 1515 1384 268
Cuts at: 8671 1632
Size: 1917
Fragments arranged by size:
1917 1515 1441 1384 1197 603 504 268
127
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8759
AflIII A'CryG_T
Cuts at: 1203 3122 7855 8832 1203
Size: 1919 4733 977 1327
Fragments arranged by size:
4733 1919 1327 977
AgeI A'CCGG_T
Cuts at: 7787 7787
Size: 8956
AhdI GACnn_n'nnGTC
Cuts at: 4810 7685 4810
Size: 2875 6081
AleI CACnn'nnGTG
Cuts at: 1166 2426 3135 5123 1166
Size: 1260 709 1988 4999
Fragments arranged by size:
4999 1988 1260 709
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2381 4275 1184
Size: 1197 1894 5865
Fragments arranged by size:
5865 1894 1197
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4389 4421 5601 5633 4389
Size: 32 1180 32 7712
Fragments arranged by size:
7712 1180 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2208 2797 3056 3629 3643 4010 4416 4840 4977
Size: 589 259 573 14 367 406 424 137
Cuts at: 4977 6699 8271 2208
Size: 1722 1572 2893
Fragments arranged by size:
2893 1722 1572 589 573 424 406 367
259 137 14
ApaI G_GGCC'C
Cuts at: 4673 5294 5646 5897 4673
Size: 621 352 251 7732
Fragments arranged by size:
7732 621 352 251
ApaLI G'TGCA_C
Cuts at: 7013 8169 7013
Size: 1156 7800
ApoI r'AATT_y
Cuts at: 1493 2819 6265 6491 6643 8752 1493
Size: 1326 3446 226 152 2109 1697
Fragments arranged by size:
3446 2109 1697 1326 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7510 160
Size: 872 6478 1606
Fragments arranged by size:
6478 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8956
AvaI C'yCGr_G
Cuts at: 1870 1943 3299 3444 4519 4704 5082 5261 5910
Size: 73 1356 145 1075 185 378 179 649
Cuts at: 5910 6281 1870
Size: 371 4545
Fragments arranged by size:
4545 1356 1075 649 378 371 185 179
145 73
AvrII C'CTAG_G
Cuts at: 2099 3655 2099
Size: 1556 7400
BamHI G'GATC_C
Cuts at: 2324 3905 6286 6653 2324
Size: 1581 2381 367 4627
Fragments arranged by size:
4627 2381 1581 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2481 3508 3945 4218 4368
Size: 325 217 627 694 1027 437 273 150
Cuts at: 4368 4487 4590 4605 4880 5015 6203 6277 7732
Size: 119 103 15 275 135 1188 74 1455
Cuts at: 7732 618
Size: 1842
Fragments arranged by size:
1842 1455 1188 1027 694 627 437 325
275 273 217 150 135 119 103 74
15
BanII G_rGCy'C
Cuts at: 1391 2062 3825 4673 5033 5177 5294 5646 5682
Size: 671 1763 848 360 144 117 352 36
Cuts at: 5682 5897 6275 1391
Size: 215 378 4072
Fragments arranged by size:
4072 1763 848 671 378 360 352 215
144 117 36
BbeI G_GCGC'C
Cuts at: 3949 5019 6207 3949
Size: 1070 1188 6698
Fragments arranged by size:
6698 1188 1070
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3277 928
Size: 971 1378 6607
Fragments arranged by size:
6607 1378 971
BbvCI CC'TCA_GC
Cuts at: 3785 4615 4822 3785
Size: 830 207 7919
Fragments arranged by size:
7919 830 207
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5063 5097 7147 7181 1903
Size: 34 3126 34 2050 34 3678
Fragments arranged by size:
3678 3126 2050 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3774 4485 5419 6848 8058 3774
Size: 711 934 1429 1210 4672
Fragments arranged by size:
4672 1429 1210 934 711
BclI T'GATC_A
Cuts at: 1548 5511 1548
Size: 3963 4993
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2261 3493 3810
Size: 107 122 71 631 605 589 1232 317
Cuts at: 3810 4347 5085 7567 136
Size: 537 738 2482 1525
Fragments arranged by size:
2482 1525 1232 738 631 605 589 537
317 122 107 71
BglII A'GATC_T
Cuts at: 2772 6114 8951 2772
Size: 3342 2837 2777
Fragments arranged by size:
3342 2837 2777
BlpI GC'TnA_GC
Cuts at: 2109 4553 6373 2109
Size: 2444 1820 4692
Fragments arranged by size:
4692 2444 1820
Bme1580I G_kGCm'C
Cuts at: 1790 3149 4221 4317 4343 4492 4608 4673 4683
Size: 1359 1072 96 26 149 116 65 10
Cuts at: 4683 5040 5294 5584 5646 5802 5897 7017 8173
Size: 357 254 290 62 156 95 1120 1156
Cuts at: 8173 1790
Size: 2573
Fragments arranged by size:
2573 1359 1156 1120 1072 357 290 254
156 149 116 96 95 65 62 26
10
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8956
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2294 4020 4199
Size: 416 53 83 399 24 877 1726 179
Cuts at: 4199 4404 4668 4934 4965 5571 5649 5667 6245
Size: 205 264 266 31 606 78 18 578
Cuts at: 6245 6687 7645 442
Size: 442 958 1753
Fragments arranged by size:
1753 1726 958 877 606 578 442 416
399 266 264 205 179 83 78 53
31 24 18
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8956
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4342 4342
Size: 8956
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2136 3054 3104 3277 3501 3890 4079 4169
Size: 897 918 50 173 224 389 189 90
Cuts at: 4169 4182 4440 4667 4681 5778 6043 7616 1239
Size: 13 258 227 14 1097 265 1573 2579
Fragments arranged by size:
2579 1573 1097 918 897 389 265 258
227 224 189 173 90 50 14 13
Bpu10I CC'TnA_GC
Cuts at: 2616 3785 4615 4822 4896 4920 5225 2616
Size: 1169 830 207 74 24 305 6347
Fragments arranged by size:
6347 1169 830 305 207 74 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5540 6057 7081 7946 8244 5540
Size: 517 1024 865 298 6252
Fragments arranged by size:
6252 1024 865 517 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3346 4704 5115 5713 5774 7619 882
Size: 617 1847 1358 411 598 61 1845 2219
Fragments arranged by size:
2219 1847 1845 1358 617 598 411 61
BsaAI yAC'GTr
Cuts at: 493 1204 2428 2698 6253 6747 493
Size: 711 1224 270 3555 494 2702
Fragments arranged by size:
3555 2702 1224 711 494 270
BsaBI GATnn'nnATC
Cuts at: 8950 8950
Size: 8956
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3946 5016 6204 6763
Size: 53 83 186 936 2413 1070 1188 559
Cuts at: 6763 7145 7780 275
Size: 382 635 1451
Fragments arranged by size:
2413 1451 1188 1070 936 635 559 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2139 3153 4883 6078 7389 7787 8061 8208 8619
Size: 1014 1730 1195 1311 398 274 147 411
Cuts at: 8619 2139
Size: 2476
Fragments arranged by size:
2476 1730 1311 1195 1014 411 398 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4389 4419 4640 4670 4942
Size: 30 165 30 2986 30 221 30 272
Cuts at: 4942 4972 5708 5738 5867 5897 6146 6176 1178
Size: 30 736 30 129 30 249 30 3958
Fragments arranged by size:
3958 2986 736 272 249 221 165 129
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2083 3086 3089 3280 3889 4118 4413 4589
Size: 897 1003 3 191 609 229 295 176
Cuts at: 4589 1186
Size: 5553
Fragments arranged by size:
5553 1003 897 609 295 229 191 176
3
BseYI C'CCAG_C
Cuts at: 2406 2859 3063 3378 3692 4093 4420 4478 4961
Size: 453 204 315 314 401 327 58 483
Cuts at: 4961 5583 5712 8159 8655 2406
Size: 622 129 2447 496 2707
Fragments arranged by size:
2707 2447 622 496 483 453 401 327
315 314 204 129 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2181 2221 2918 3172 3500 4184 4742
Size: 573 445 40 697 254 328 684 558
Cuts at: 4742 1163
Size: 5377
Fragments arranged by size:
5377 697 684 573 558 445 328 254
40
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3614 3959 6406 7167 8195 1540
Size: 275 186 1613 345 2447 761 1028 2301
Fragments arranged by size:
2447 2301 1613 1028 761 345 275 186
BsiHKAI G_wGCw'C
Cuts at: 2319 2651 2946 5177 5816 5859 7017 7102 8173
Size: 332 295 2231 639 43 1158 85 1071
Cuts at: 8173 2319
Size: 3102
Fragments arranged by size:
3102 2231 1158 1071 639 332 295 85
43
BsmI GAATG_Cn'
Cuts at: 5754 6467 6560 5754
Size: 713 93 8150
Fragments arranged by size:
8150 713 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3695 3695
Size: 8956
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2062 2319 2651 2946 3149 3825 4221
Size: 399 272 257 332 295 203 676 396
Cuts at: 4221 4317 4343 4492 4608 4673 4683 5033 5040
Size: 96 26 149 116 65 10 350 7
Cuts at: 5040 5177 5294 5584 5646 5682 5802 5816 5859
Size: 137 117 290 62 36 120 14 43
Cuts at: 5859 5897 6275 7017 7102 8173 1391
Size: 38 378 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 676 399 396 378 350
332 295 290 272 257 203 149 137
120 117 116 96 85 65 62 43
38 36 26 14 10 7
BspEI T'CCGG_A
Cuts at: 2139 8619 2139
Size: 6480 2476
BspHI T'CATG_A
Cuts at: 6845 8575 6845
Size: 1730 7226
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2502 2550 3194 4341 5194 5265 6311 844
Size: 1658 48 644 1147 853 71 1046 3489
Fragments arranged by size:
3489 1658 1147 1046 853 644 71 48
BsrBI CCG'CTC
Cuts at: 1394 1823 2392 2401 6843 1394
Size: 429 569 9 4442 3507
Fragments arranged by size:
4442 3507 569 429 9
BsrDI GCAATG_nn'
Cuts at: 65 1248 3045 4595 5413 5472 7451 7625 65
Size: 1183 1797 1550 818 59 1979 174 1396
Fragments arranged by size:
1979 1797 1550 1396 1183 818 174 59
BsrFI r'CCGG_y
Cuts at: 1700 1810 3589 3609 3852 4651 7600 7787 1700
Size: 110 1779 20 243 799 2949 187 2869
Fragments arranged by size:
2949 2869 1779 799 243 187 110 20
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8956
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8956
BssSI C'ACGA_G
Cuts at: 3161 3410 4316 7016 8028 3161
Size: 249 906 2700 1012 4089
Fragments arranged by size:
4089 2700 1012 906 249
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2208 2941 4207 4225 794
Size: 1414 733 1266 18 5525
Fragments arranged by size:
5525 1414 1266 733 18
BstBI TT'CG_AA
Cuts at: 2007 7760 2007
Size: 5753 3203
BstEII G'GTnAC_C
Cuts at: 1184 2173 1184
Size: 989 7967
BstXI CCAn_nnnn'nTGG
Cuts at: 3779 5012 5286 3779
Size: 1233 274 7449
Fragments arranged by size:
7449 1233 274
BstYI r'GATC_y
Cuts at: 1972 2324 2772 3905 4563 5767 6114 6286 6653
Size: 352 448 1133 658 1204 347 172 367
Cuts at: 6653 7038 7055 8507 8593 8605 8951 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1204 1133 658 448 385 367
352 347 346 172 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4983 4983
Size: 8956
BtgI C'CryG_G
Cuts at: 513 1065 2274 2664 3196 3273 3323 4499 5604
Size: 552 1209 390 532 77 50 1176 1105
Cuts at: 5604 513
Size: 3865
Fragments arranged by size:
3865 1209 1176 1105 552 532 390 77
50
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3123 5292 487
Size: 683 56 1897 2169 4151
Fragments arranged by size:
4151 2169 1897 683 56
BtsI GCAGTG_nn'
Cuts at: 3793 4688 4726 6472 7266 7286 3793
Size: 895 38 1746 794 20 5463
Fragments arranged by size:
5463 1746 895 794 38 20
ClaI AT'CG_AT
Cuts at: 1554 2181 1554
Size: 627 8329
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8921
DraI TTT'AAA
Cuts at: 6260 6260
Size: 8956
DraIII CAC_nnn'GTG
Cuts at: 2941 4207 4338 5579 2941
Size: 1266 131 1241 6318
Fragments arranged by size:
6318 1266 1241 131
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3338 7963 809
Size: 507 2022 4625 1802
Fragments arranged by size:
4625 2022 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2445 2799 2838
Size: 54 1425 50 275 18 615 354 39
Cuts at: 2838 3139 3607 3611 3672 3705 3854 4429 5151
Size: 301 468 4 61 33 149 575 722
Cuts at: 5151 5562 6129 6403 7292 8921 8
Size: 411 567 274 889 1629 43
Fragments arranged by size:
1629 1425 889 722 615 575 567 468
411 354 301 275 274 149 61 54
50 43 39 33 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 3611 6403 1537
Size: 275 1799 2792 4090
Fragments arranged by size:
4090 2792 1799 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2552 2933 3717 3935 5772 6023 6886 1353
Size: 1199 381 784 218 1837 251 863 3423
Fragments arranged by size:
3423 1837 1199 863 784 381 251 218
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2801 3756 5048 5609 5689 7543
Size: 1064 138 1212 955 1292 561 80 1854
Cuts at: 7543 7917 8063 387
Size: 374 146 1280
Fragments arranged by size:
1854 1292 1280 1212 1064 955 561 374
146 138 80
EcoICRI GAG'CTC
Cuts at: 5175 5175
Size: 8956
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2136 2235 2362 2866 3054 3104 3277
Size: 393 504 99 127 504 188 50 173
Cuts at: 3277 3501 3890 4079 4169 4182 4307 4440 4667
Size: 224 389 189 90 13 125 133 227
Cuts at: 4667 4681 5504 5778 6043 7019 7616 8403 8671
Size: 14 823 274 265 976 597 787 268
Cuts at: 8671 1239
Size: 1524
Fragments arranged by size:
1524 976 823 787 597 504 504 393
389 274 268 265 227 224 189 188
173 133 127 125 99 90 50 14
13
EcoNI CCTnn'n_nnAGG
Cuts at: 2976 5483 5834 2976
Size: 2507 351 6098
Fragments arranged by size:
6098 2507 351
EcoO109I rG'GnC_Cy
Cuts at: 3053 3449 3746 4063 4622 4669 4670 4812 4891
Size: 396 297 317 559 47 1 142 79
Cuts at: 4891 5078 5087 5282 5290 5494 5502 5599 5894
Size: 187 9 195 8 204 8 97 295
Cuts at: 5894 3053
Size: 6115
Fragments arranged by size:
6115 559 396 317 297 295 204 195
187 142 97 79 47 9 8 8
1
EcoRI G'AATT_C
Cuts at: 6265 6643 6265
Size: 378 8578
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 6037 6037
Size: 8956
FseI GG_CCGG'CC
Cuts at: 3613 3613
Size: 8956
FspI TGC'GCA
Cuts at: 5753 7462 5753
Size: 1709 7247
HaeII r_GCGC'y
Cuts at: 1678 2407 3949 4118 4829 5019 6207 8103 1678
Size: 729 1542 169 711 190 1188 1896 2531
Fragments arranged by size:
2531 1896 1542 1188 729 711 190 169
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3192 3224 4640 4672 4796
Size: 32 135 32 1620 32 1416 32 124
Cuts at: 4796 4828 4860 4889 4921 4942 4974 5288 5320
Size: 32 32 29 32 21 32 314 32
Cuts at: 5320 5867 5899 7597 7629 7671 7703 1373
Size: 547 32 1698 32 42 32 2626
Fragments arranged by size:
2626 1698 1620 1416 547 314 135 124
42 32 32 32 32 32 32 32
32 32 32 32 32 29 21
HincII GTy'rAC
Cuts at: 669 1657 1938 3349 6300 6546 669
Size: 988 281 1411 2951 246 3079
Fragments arranged by size:
3079 2951 1411 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2732 748
Size: 1984 6972
HpaI GTT'AAC
Cuts at: 6546 6546
Size: 8956
KasI G'GCGC_C
Cuts at: 3945 5015 6203 3945
Size: 1070 1188 6698
Fragments arranged by size:
6698 1188 1070
KpnI G_GTAC'C
Cuts at: 4372 4594 6281 4372
Size: 222 1687 7047
Fragments arranged by size:
7047 1687 222
MfeI C'AATT_G
Cuts at: 6555 6555
Size: 8956
MluI A'CGCG_T
Cuts at: 8832 8832
Size: 8956
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4973 8070 8254 1305
Size: 3668 3097 184 2007
Fragments arranged by size:
3668 3097 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2801 2840 3674 5153 5564
Size: 54 1425 343 969 39 834 1479 411
Cuts at: 5564 6131 10
Size: 567 2835
Fragments arranged by size:
2835 1479 1425 969 834 567 411 343
54 39
MslI CAynn'nnrTG
Cuts at: 518 1166 2426 2909 2951 3135 4257 4446 5010
Size: 648 1260 483 42 184 1122 189 564
Cuts at: 5010 5123 5807 6914 7273 7432 518
Size: 113 684 1107 359 159 2042
Fragments arranged by size:
2042 1260 1122 1107 684 648 564 483
359 189 184 159 113 42
MspA1I CmG'CkG
Cuts at: 1606 2034 2479 3484 3626 4225 4281 5614 5819
Size: 428 445 1005 142 599 56 1333 205
Cuts at: 5819 5957 6372 7049 8197 8442 1606
Size: 138 415 677 1148 245 2120
Fragments arranged by size:
2120 1333 1148 1005 677 599 445 428
415 245 205 142 138 56
NaeI GCC'GGC
Cuts at: 3591 3611 3591
Size: 20 8936
NarI GG'CG_CC
Cuts at: 3946 5016 6204 3946
Size: 1070 1188 6698
Fragments arranged by size:
6698 1188 1070
NcoI C'CATG_G
Cuts at: 513 1065 2664 513
Size: 552 1599 6805
Fragments arranged by size:
6805 1599 552
NdeI CA'TA_TG
Cuts at: 387 4426 387
Size: 4039 4917
NgoMIV G'CCGG_C
Cuts at: 3589 3609 3589
Size: 20 8936
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8956
NotI GC'GGCC_GC
Cuts at: 6403 6403
Size: 8956
NspI r_CATG'y
Cuts at: 1132 1229 2627 2761 3126 6314 7859 1132
Size: 97 1398 134 365 3188 1545 2229
Fragments arranged by size:
3188 2229 1545 1398 365 134 97
PasI CC'CwG_GG
Cuts at: 1348 2062 3913 4067 5286 5498 5983 6150 1348
Size: 714 1851 154 1219 212 485 167 4154
Fragments arranged by size:
4154 1851 1219 714 485 212 167 154
PciI A'CATG_T
Cuts at: 3122 7855 3122
Size: 4733 4223
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2797 4010 4427 4505 4601 4977 5439 5905
Size: 1689 1213 417 78 96 376 462 466
Cuts at: 5905 1108
Size: 4159
Fragments arranged by size:
4159 1689 1213 466 462 417 376 96
78
PfoI T'CCnGG_A
Cuts at: 2124 4056 4456 4658 5421 2124
Size: 1932 400 202 763 5659
Fragments arranged by size:
5659 1932 763 400 202
PmeI GTTT'AAAC
Cuts at: 6260 6260
Size: 8956
PmlI CAC'GTG
Cuts at: 2698 2698
Size: 8956
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3320 3352 4389 4421 7025 7057 8538 8570 3320
Size: 32 1037 32 2604 32 1481 32 3706
Fragments arranged by size:
3706 2604 1481 1037 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3449 4063 4622 4812 5282 5494 5599 3449
Size: 614 559 190 470 212 105 6806
Fragments arranged by size:
6806 614 559 470 212 190 105
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8956
PsiI TTA'TAA
Cuts at: 6526 6526
Size: 8956
PspOMI G'GGCC_C
Cuts at: 4669 5290 5642 5893 4669
Size: 621 352 251 7732
Fragments arranged by size:
7732 621 352 251
PspXI vC'TCGA_Gb
Cuts at: 5082 5082
Size: 8956
PstI C_TGCA'G
Cuts at: 830 2782 2853 4639 5852 5926 6308 830
Size: 1952 71 1786 1213 74 382 3478
Fragments arranged by size:
3478 1952 1786 1213 382 74 71
PvuI CG_AT'CG
Cuts at: 2001 3959 2001
Size: 1958 6998
PvuII CAG'CTG
Cuts at: 3626 4281 5614 5957 3626
Size: 655 1333 343 6625
Fragments arranged by size:
6625 1333 655 343
SacI G_AGCT'C
Cuts at: 5177 5177
Size: 8956
SalI G'TCGA_C
Cuts at: 1936 3347 6298 1936
Size: 1411 2951 4594
Fragments arranged by size:
4594 2951 1411
SanDI GG'GwC_CC
Cuts at: 3449 5599 3449
Size: 2150 6806
SapI GCTCTTCn'nnn_
Cuts at: 2552 3717 6023 2552
Size: 1165 2306 5485
Fragments arranged by size:
5485 2306 1165
SbfI CC_TGCA'GG
Cuts at: 6308 6308
Size: 8956
ScaI AGT'ACT
Cuts at: 7204 8916 7204
Size: 1712 7244
SexAI A'CCwGG_T
Cuts at: 4808 4808
Size: 8956
SfcI C'TryA_G
Cuts at: 826 1045 2764 2778 2849 3621 4122 4276 4635
Size: 219 1719 14 71 772 501 154 359
Cuts at: 4635 5848 5922 5935 6304 7439 8120 8311 826
Size: 1213 74 13 369 1135 681 191 1471
Fragments arranged by size:
1719 1471 1213 1135 772 681 501 369
359 219 191 154 74 71 14 13
SfiI GGCCn_nnn'nGGCC
Cuts at: 5085 5085
Size: 8956
SfoI GGC'GCC
Cuts at: 3947 5017 6205 3947
Size: 1070 1188 6698
Fragments arranged by size:
6698 1188 1070
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8956
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8956
SmaI CCC'GGG
Cuts at: 1872 3446 4521 5263 5912 6283 1872
Size: 1574 1075 742 649 371 4545
Fragments arranged by size:
4545 1574 1075 742 649 371
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5082 5555 6072 7060 7961 8223
Size: 197 926 3139 473 517 988 901 262
Cuts at: 8223 820
Size: 1553
Fragments arranged by size:
3139 1553 988 926 901 517 473 262
197
SnaBI TAC'GTA
Cuts at: 493 6253 6747 493
Size: 5760 494 2702
Fragments arranged by size:
5760 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8956
SphI G_CATG'C
Cuts at: 1132 2627 6314 1132
Size: 1495 3687 3774
Fragments arranged by size:
3774 3687 1495
SrfI GCCC'GGGC
Cuts at: 5263 5912 5263
Size: 649 8307
SspI AAT'ATT
Cuts at: 5 52 6880 5
Size: 47 6828 2081
Fragments arranged by size:
6828 2081 47
StuI AGG'CCT
Cuts at: 1517 2098 2264 3239 3778 3929 4856 1517
Size: 581 166 975 539 151 927 5617
Fragments arranged by size:
5617 975 927 581 539 166 151
StyI C'CwwG_G
Cuts at: 513 1065 2099 2592 2664 3219 3655 4296 5533
Size: 552 1034 493 72 555 436 641 1237
Cuts at: 5533 5703 6395 513
Size: 170 692 3074
Fragments arranged by size:
3074 1237 1034 692 641 555 552 493
436 170 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3328 4218 4309 4686 5590 6983 7000
Size: 1255 1748 890 91 377 904 1393 17
Cuts at: 7000 7153 7338 7783 325
Size: 153 185 445 1498
Fragments arranged by size:
1748 1498 1393 1255 904 890 445 377
185 153 91 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 7202 8914 96
Size: 274 80 33 51 1443 5225 1712 138
Fragments arranged by size:
5225 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2309 2341 2630 2662 3433 3465 4753 4785 5032
Size: 32 289 32 771 32 1288 32 247
Cuts at: 5032 5064 5175 5207 5708 5740 6146 6178 8916
Size: 32 111 32 501 32 406 32 2738
Cuts at: 8916 8948 2309
Size: 32 2317
Fragments arranged by size:
2738 2317 1288 771 501 406 289 247
111 32 32 32 32 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4842 5881 1316
Size: 3526 1039 4391
Fragments arranged by size:
4391 3526 1039
XbaI T'CTAG_A
Cuts at: 6292 6292
Size: 8956
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2038 3375 5540 6232 2038
Size: 1337 2165 692 4762
Fragments arranged by size:
4762 2165 1337 692
XhoI C'TCGA_G
Cuts at: 1943 5082 1943
Size: 3139 5817
XmaI C'CCGG_G
Cuts at: 1870 3444 4519 5261 5910 6281 1870
Size: 1574 1075 742 649 371 4545
Fragments arranged by size:
4545 1574 1075 742 649 371
XmnI GAAnn'nnTTC
Cuts at: 2135 6017 7085 2135
Size: 3882 1068 4006
Fragments arranged by size:
4006 3882 1068
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6764 276
Size: 53 83 186 936 5230 2468
Fragments arranged by size:
5230 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BfrBI BsiWI BstZ17I EcoRV FspAI
NruI NsiI PacI PsrI RsrII SacII SwaI