Restriction map for
pFN21ASDA1073
(Circular) (Six-base) MAPSORT of: pFN21ASDA1073.seq Check: 9251 from: 1 to: 6658
pFN21ASDA1073 6658 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3257 4468 278
Size: 53 83 186 936 1721 1211 2468
Fragments arranged by size:
2468 1721 1211 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3951 4001 1937
Size: 2014 50 4594
Fragments arranged by size:
4594 2014 50
Acc65I G'GTAC_C
Cuts at: 3979 3979
Size: 6658
AclI AA'CG_TT
Cuts at: 1563 4785 5158 1563
Size: 3222 373 3063
Fragments arranged by size:
3222 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2788 3827 4721 6105 6373 1632
Size: 1156 1039 894 1384 268 1917
Fragments arranged by size:
1917 1384 1156 1039 894 268
AflII C'TTAA_G
Cuts at: 820 1017 3119 820
Size: 197 2102 4359
Fragments arranged by size:
4359 2102 197
AflIII A'CryG_T
Cuts at: 1203 5557 6534 1203
Size: 4354 977 1327
Fragments arranged by size:
4354 1327 977
AgeI A'CCGG_T
Cuts at: 5489 5489
Size: 6658
AhdI GACnn_n'nnGTC
Cuts at: 2095 5387 2095
Size: 3292 3366
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6658
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6658
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3054 3086 3054
Size: 32 6626
AlwNI CAG_nnn'CTG
Cuts at: 2150 4401 5973 2150
Size: 2251 1572 2835
Fragments arranged by size:
2835 2251 1572
ApaLI G'TGCA_C
Cuts at: 4715 5871 4715
Size: 1156 5502
ApoI r'AATT_y
Cuts at: 1493 2125 2461 2500 2679 3518 3967 4193 4345
Size: 632 336 39 179 839 449 226 152
Cuts at: 4345 6454 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 839 632 449 336 226 179
152 39
AseI AT'TA_AT
Cuts at: 160 1032 5212 160
Size: 872 4180 1606
Fragments arranged by size:
4180 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6658
AvaI C'yCGr_G
Cuts at: 1870 1943 2035 3983 1870
Size: 73 92 1948 4545
Fragments arranged by size:
4545 1948 92 73
BamHI G'GATC_C
Cuts at: 2380 3988 4355 2380
Size: 1608 367 4683
Fragments arranged by size:
4683 1608 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3979 5434 618
Size: 325 217 627 2192 1455 1842
Fragments arranged by size:
2192 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2036 3220 3747 3977 1391
Size: 645 1184 527 230 4072
Fragments arranged by size:
4072 1184 645 527 230
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3257 3675 928
Size: 971 1358 418 3911
Fragments arranged by size:
3911 1358 971 418
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3432 3466 4849 4883 1903
Size: 34 1495 34 1383 34 3678
Fragments arranged by size:
3678 1495 1383 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2831 3326 4550 5760 2831
Size: 495 1224 1210 3729
Fragments arranged by size:
3729 1224 1210 495
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6658
BfrBI ATG'CAT
Cuts at: 2570 2570
Size: 6658
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3014 5269 136
Size: 107 122 71 631 605 1342 2255 1525
Fragments arranged by size:
2255 1525 1342 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6653 6653
Size: 6658
BlpI GC'TnA_GC
Cuts at: 3297 4075 3297
Size: 778 5880
Bme1580I G_kGCm'C
Cuts at: 1790 4719 5875 1790
Size: 2929 1156 2573
Fragments arranged by size:
2929 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6658
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2819 2950 4389
Size: 416 53 83 399 24 1402 131 1439
Cuts at: 4389 5347 442
Size: 958 1753
Fragments arranged by size:
1753 1439 1402 958 416 399 131 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6658
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2050 2050
Size: 6658
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2716 5318 1239
Size: 1477 2602 2579
Fragments arranged by size:
2602 2579 1477
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4783 5648 5946 4783
Size: 865 298 5495
Fragments arranged by size:
5495 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5321 882
Size: 617 3822 2219
Fragments arranged by size:
3822 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3955 4449 493
Size: 711 2751 494 2702
Fragments arranged by size:
2751 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 3048 6652 3048
Size: 3604 3054
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3254 4465 4847 5482
Size: 53 83 186 936 1721 1211 382 635
Cuts at: 5482 275
Size: 1451
Fragments arranged by size:
1721 1451 1211 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5091 5489 5763 5910 6321 5091
Size: 398 274 147 411 5428
Fragments arranged by size:
5428 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3116 3146 1178
Size: 30 165 30 1713 30 4690
Fragments arranged by size:
4690 1713 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6658
BseYI C'CCAG_C
Cuts at: 2058 2081 5861 6357 2058
Size: 23 3780 496 2359
Fragments arranged by size:
3780 2359 496 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3903 1163
Size: 573 2167 3918
Fragments arranged by size:
3918 2167 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2078 4108 4869 5897 1540
Size: 275 186 77 2030 761 1028 2301
Fragments arranged by size:
2301 2030 1028 761 275 186 77
BsiHKAI G_wGCw'C
Cuts at: 2036 3747 4719 4804 5875 2036
Size: 1711 972 85 1071 2819
Fragments arranged by size:
2819 1711 1071 972 85
BsmI GAATG_Cn'
Cuts at: 4169 4262 4169
Size: 93 6565
BsmBI CGTCTCn'nnnn_
Cuts at: 2021 2021
Size: 6658
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2036 3220 3747 3977 4719 4804 5875
Size: 399 246 1184 527 230 742 85 1071
Cuts at: 5875 1391
Size: 2174
Fragments arranged by size:
2174 1184 1071 742 527 399 246 230
85
BspEI T'CCGG_A
Cuts at: 6321 6321
Size: 6658
BspHI T'CATG_A
Cuts at: 4547 6277 4547
Size: 1730 4928
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2598 4013 844
Size: 1754 1415 3489
Fragments arranged by size:
3489 1754 1415
BsrBI CCG'CTC
Cuts at: 1394 1823 4545 1394
Size: 429 2722 3507
Fragments arranged by size:
3507 2722 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3268 5153 5327 65
Size: 1183 2020 1885 174 1396
Fragments arranged by size:
2020 1885 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2062 5302 5489 1700
Size: 110 252 3240 187 2869
Fragments arranged by size:
3240 2869 252 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6658
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6658
BssSI C'ACGA_G
Cuts at: 4718 5730 4718
Size: 1012 5646
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6658
BstBI TT'CG_AA
Cuts at: 2007 5462 2007
Size: 3455 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6658
BstYI r'GATC_y
Cuts at: 1972 2380 2540 3988 4355 4740 4757 6209 6295
Size: 408 160 1448 367 385 17 1452 86
Cuts at: 6295 6307 6653 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1448 408 385 367 346 160
86 17 12
BstZ17I GTA'TAC
Cuts at: 3952 3952
Size: 6658
Bsu36I CC'TnA_GG
Cuts at: 2213 2523 2213
Size: 310 6348
BtgI C'CryG_G
Cuts at: 513 1065 2022 2883 3418 3776 513
Size: 552 957 861 535 358 3395
Fragments arranged by size:
3395 957 861 552 535 358
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5919
Fragments arranged by size:
5919 683 56
BtsI GCAGTG_nn'
Cuts at: 3280 4174 4968 4988 3280
Size: 894 794 20 4950
Fragments arranged by size:
4950 894 794 20
ClaI AT'CG_AT
Cuts at: 1554 3397 1554
Size: 1843 4815
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6623
DraI TTT'AAA
Cuts at: 3962 3962
Size: 6658
DraIII CAC_nnn'GTG
Cuts at: 2871 3926 2871
Size: 1055 5603
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5665 809
Size: 507 4349 1802
Fragments arranged by size:
4349 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2075 2283 2987
Size: 54 1425 50 275 18 245 208 704
Cuts at: 2987 3415 4105 4994 6623 8
Size: 428 690 889 1629 43
Fragments arranged by size:
1629 1425 889 704 690 428 275 245
208 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2075 4105 1537
Size: 275 263 2030 4090
Fragments arranged by size:
4090 2030 275 263
EarI CTCTTCn'nnn_
Cuts at: 1353 4588 1353
Size: 3235 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2545 5245 5619 5765 387
Size: 1064 138 956 2700 374 146 1280
Fragments arranged by size:
2700 1280 1064 956 374 146 138
EcoICRI GAG'CTC
Cuts at: 2034 3745 2034
Size: 1711 4947
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2716 2788 3827 4721 5318 6105 6373
Size: 393 1084 72 1039 894 597 787 268
Cuts at: 6373 1239
Size: 1524
Fragments arranged by size:
1524 1084 1039 894 787 597 393 268
72
EcoO109I rG'GnC_Cy
Cuts at: 2209 2209
Size: 6658
EcoRI G'AATT_C
Cuts at: 2125 2679 3518 3967 4345 2125
Size: 554 839 449 378 4438
Fragments arranged by size:
4438 839 554 449 378
EcoRV GAT'ATC
Cuts at: 3079 3079
Size: 6658
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3635 3839 3635
Size: 204 6454
FspI TGC'GCA
Cuts at: 5164 5164
Size: 6658
HaeII r_GCGC'y
Cuts at: 1678 2322 5805 1678
Size: 644 3483 2531
Fragments arranged by size:
3483 2531 644
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2081 2113 2805 2837 3345
Size: 32 135 32 509 32 692 32 508
Cuts at: 3345 3377 5299 5331 5373 5405 1373
Size: 32 1922 32 42 32 2626
Fragments arranged by size:
2626 1922 692 509 508 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4002 4248 669
Size: 988 281 2064 246 3079
Fragments arranged by size:
3079 2064 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3200 748
Size: 2452 4206
HpaI GTT'AAC
Cuts at: 4248 4248
Size: 6658
KpnI G_GTAC'C
Cuts at: 3983 3983
Size: 6658
MfeI C'AATT_G
Cuts at: 4257 4257
Size: 6658
MluI A'CGCG_T
Cuts at: 6534 6534
Size: 6658
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2869 3306 5772 5956 1305
Size: 1564 437 2466 184 2007
Fragments arranged by size:
2466 2007 1564 437 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2285 3417 10
Size: 54 1425 343 453 1132 3251
Fragments arranged by size:
3251 1425 1132 453 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3273 3320 3438 4616 4975 5134 518
Size: 648 2107 47 118 1178 359 159 2042
Fragments arranged by size:
2107 2042 1178 648 359 159 118 47
MspA1I CmG'CkG
Cuts at: 1606 3564 3664 4074 4751 5899 6144 1606
Size: 1958 100 410 677 1148 245 2120
Fragments arranged by size:
2120 1958 1148 677 410 245 100
NaeI GCC'GGC
Cuts at: 2064 2064
Size: 6658
NcoI C'CATG_G
Cuts at: 513 1065 2022 2883 3418 513
Size: 552 957 861 535 3753
Fragments arranged by size:
3753 957 861 552 535
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6658
NgoMIV G'CCGG_C
Cuts at: 2062 2062
Size: 6658
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6658
NotI GC'GGCC_GC
Cuts at: 4105 4105
Size: 6658
NsiI A_TGCA'T
Cuts at: 2572 2572
Size: 6658
NspI r_CATG'y
Cuts at: 1132 1229 3733 4016 5561 1132
Size: 97 2504 283 1545 2229
Fragments arranged by size:
2504 2229 1545 283 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6658
PciI A'CATG_T
Cuts at: 5557 5557
Size: 6658
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2315 2884 1108
Size: 1207 569 4882
Fragments arranged by size:
4882 1207 569
PfoI T'CCnGG_A
Cuts at: 2260 3072 2260
Size: 812 5846
PmeI GTTT'AAAC
Cuts at: 3962 3962
Size: 6658
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4727 4759 6240 6272 4727
Size: 32 1481 32 5113
Fragments arranged by size:
5113 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2209 2209
Size: 6658
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6658
PsiI TTA'TAA
Cuts at: 4228 4228
Size: 6658
PspXI vC'TCGA_Gb
Cuts at: 2035 2035
Size: 6658
PstI C_TGCA'G
Cuts at: 830 4010 830
Size: 3180 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6658
PvuII CAG'CTG
Cuts at: 3564 3664 3564
Size: 100 6558
SacI G_AGCT'C
Cuts at: 2036 3747 2036
Size: 1711 4947
SalI G'TCGA_C
Cuts at: 1936 4000 1936
Size: 2064 4594
SbfI CC_TGCA'GG
Cuts at: 4010 4010
Size: 6658
ScaI AGT'ACT
Cuts at: 4906 6618 4906
Size: 1712 4946
SfcI C'TryA_G
Cuts at: 826 1045 3863 4006 5141 5822 6013 826
Size: 219 2818 143 1135 681 191 1471
Fragments arranged by size:
2818 1471 1135 681 219 191 143
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6658
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6658
SmaI CCC'GGG
Cuts at: 1872 3985 1872
Size: 2113 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2035 3119 4762 5663 5925 820
Size: 197 926 92 1084 1643 901 262 1553
Fragments arranged by size:
1643 1553 1084 926 901 262 197 92
SnaBI TAC'GTA
Cuts at: 493 3955 4449 493
Size: 3462 494 2702
Fragments arranged by size:
3462 2702 494
SpeI A'CTAG_T
Cuts at: 152 3064 152
Size: 2912 3746
SphI G_CATG'C
Cuts at: 1132 3733 4016 1132
Size: 2601 283 3774
Fragments arranged by size:
3774 2601 283
SspI AAT'ATT
Cuts at: 5 52 2272 2770 3088 4582 5
Size: 47 2220 498 318 1494 2081
Fragments arranged by size:
2220 2081 1494 498 318 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6658
StyI C'CwwG_G
Cuts at: 513 1065 2022 2407 2883 3418 4097 513
Size: 552 957 385 476 535 679 3074
Fragments arranged by size:
3074 957 679 552 535 476 385
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2625 3739 4685 4702 4855 5040 5485
Size: 1255 1045 1114 946 17 153 185 445
Cuts at: 5485 325
Size: 1498
Fragments arranged by size:
1498 1255 1114 1045 946 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4904 6616 96
Size: 274 80 33 51 1443 2927 1712 138
Fragments arranged by size:
2927 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6618 6650 6618
Size: 32 6626
Tth111I GACn'n_nGTC
Cuts at: 1316 3479 3634 1316
Size: 2163 155 4340
Fragments arranged by size:
4340 2163 155
XbaI T'CTAG_A
Cuts at: 3994 3994
Size: 6658
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2691 2832 2885 3322 2691
Size: 141 53 437 6027
Fragments arranged by size:
6027 437 141 53
XhoI C'TCGA_G
Cuts at: 1943 2035 1943
Size: 92 6566
XmaI C'CCGG_G
Cuts at: 1870 3983 1870
Size: 2113 4545
XmnI GAAnn'nnTTC
Cuts at: 3798 4787 3798
Size: 989 5669
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3255 4466 276
Size: 53 83 186 936 1721 1211 2468
Fragments arranged by size:
2468 1721 1211 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspXI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsiWI BstXI EcoNI FseI FspAI KasI NarI
NruI PacI PmlI PspOMI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI