Restriction map for
pFN21ASDA1072
(Circular) (Six-base) MAPSORT of: pFN21ASDA1072.seq Check: 7788 from: 1 to: 7000
pFN21ASDA1072 7000 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4810 278
Size: 53 83 186 936 3274 2468
Fragments arranged by size:
3274 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4343 1937
Size: 2406 4594
Acc65I G'GTAC_C
Cuts at: 4321 4321
Size: 7000
AclI AA'CG_TT
Cuts at: 1563 5127 5500 1563
Size: 3564 373 3063
Fragments arranged by size:
3564 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2534 2629 5063 6447 6715 1632
Size: 902 95 2434 1384 268 1917
Fragments arranged by size:
2434 1917 1384 902 268 95
AflII C'TTAA_G
Cuts at: 820 1017 3004 820
Size: 197 1987 4816
Fragments arranged by size:
4816 1987 197
AflIII A'CryG_T
Cuts at: 1203 2770 3055 5899 6876 1203
Size: 1567 285 2844 977 1327
Fragments arranged by size:
2844 1567 1327 977 285
AgeI A'CCGG_T
Cuts at: 5831 5831
Size: 7000
AhdI GACnn_n'nnGTC
Cuts at: 5729 5729
Size: 7000
AleI CACnn'nnGTG
Cuts at: 1166 3023 1166
Size: 1857 5143
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7000
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4165 4197 4165
Size: 32 6968
AlwNI CAG_nnn'CTG
Cuts at: 3281 4743 6315 3281
Size: 1462 1572 3966
Fragments arranged by size:
3966 1572 1462
ApaLI G'TGCA_C
Cuts at: 3813 5057 6213 3813
Size: 1244 1156 4600
Fragments arranged by size:
4600 1244 1156
ApoI r'AATT_y
Cuts at: 1493 4309 4535 4687 6796 1493
Size: 2816 226 152 2109 1697
Fragments arranged by size:
2816 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3837 5554 160
Size: 872 2805 1717 1606
Fragments arranged by size:
2805 1717 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7000
AvaI C'yCGr_G
Cuts at: 1870 1943 2156 2168 2225 2288 2353 4006 4325
Size: 73 213 12 57 63 65 1653 319
Cuts at: 4325 1870
Size: 4545
Fragments arranged by size:
4545 1653 319 213 73 65 63 57
12
AvrII C'CTAG_G
Cuts at: 3646 3646
Size: 7000
BamHI G'GATC_C
Cuts at: 4330 4697 4330
Size: 367 6633
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3194 4321 5776 618
Size: 325 217 627 1407 1127 1455 1842
Fragments arranged by size:
1842 1455 1407 1127 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2169 2175 2289 3698 4319 1391
Size: 778 6 114 1409 621 4072
Fragments arranged by size:
4072 1409 778 621 114 6
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2635 3315 3729 928
Size: 971 736 680 414 4199
Fragments arranged by size:
4199 971 736 680 414
BbvCI CC'TCA_GC
Cuts at: 2196 2937 3618 2196
Size: 741 681 5578
Fragments arranged by size:
5578 741 681
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5191 5225 1903
Size: 34 3254 34 3678
Fragments arranged by size:
3678 3254 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4892 6102 4892
Size: 1210 5790
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7000
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4195 5611 136
Size: 107 122 71 631 605 2523 1416 1525
Fragments arranged by size:
2523 1525 1416 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3739 4267 6995 3739
Size: 528 2728 3744
Fragments arranged by size:
3744 2728 528
BlpI GC'TnA_GC
Cuts at: 3631 4417 3631
Size: 786 6214
Bme1580I G_kGCm'C
Cuts at: 1790 2361 3778 3817 5061 6217 1790
Size: 571 1417 39 1244 1156 2573
Fragments arranged by size:
2573 1417 1244 1156 571 39
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7000
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3772 4731 5689
Size: 416 53 83 399 24 2355 959 958
Cuts at: 5689 442
Size: 1753
Fragments arranged by size:
2355 1753 959 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7000
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3981 3981
Size: 7000
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2087 3220 3918 5660 1239
Size: 848 1133 698 1742 2579
Fragments arranged by size:
2579 1742 1133 848 698
Bpu10I CC'TnA_GC
Cuts at: 2196 2937 3618 3692 2196
Size: 741 681 74 5504
Fragments arranged by size:
5504 741 681 74
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4092 5125 5990 6288 4092
Size: 1033 865 298 4804
Fragments arranged by size:
4804 1033 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2695 5663 882
Size: 617 1196 2968 2219
Fragments arranged by size:
2968 2219 1196 617
BsaAI yAC'GTr
Cuts at: 493 1204 4297 4791 493
Size: 711 3093 494 2702
Fragments arranged by size:
3093 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 6994 6994
Size: 7000
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4807 5189 5824 275
Size: 53 83 186 936 3274 382 635 1451
Fragments arranged by size:
3274 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5433 5831 6105 6252 6663 5433
Size: 398 274 147 411 5770
Fragments arranged by size:
5770 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3165 3195 1178
Size: 30 165 30 1762 30 4983
Fragments arranged by size:
4983 1762 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2057 2246 2273 2306 2309 2330 2333 2336
Size: 871 189 27 33 3 21 3 3
Cuts at: 2336 2339 2456 1186
Size: 3 117 5730
Fragments arranged by size:
5730 871 189 117 33 27 21 3
3 3 3
BseYI C'CCAG_C
Cuts at: 2385 2673 2706 3452 6203 6699 2385
Size: 288 33 746 2751 496 2686
Fragments arranged by size:
2751 2686 746 496 288 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2550 2968 3002 1163
Size: 573 814 418 34 5161
Fragments arranged by size:
5161 814 573 418 34
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2205 2367 2417 4450 5211 6239
Size: 275 186 204 162 50 2033 761 1028
Cuts at: 6239 1540
Size: 2301
Fragments arranged by size:
2301 2033 1028 761 275 204 186 162
50
BsiHKAI G_wGCw'C
Cuts at: 3632 3817 5061 5146 6217 3632
Size: 185 1244 85 1071 4415
Fragments arranged by size:
4415 1244 1071 185 85
BsmI GAATG_Cn'
Cuts at: 2778 3957 4511 4604 2778
Size: 1179 554 93 5174
Fragments arranged by size:
5174 1179 554 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2169 2175 2289 2361 3632 3698 3778
Size: 399 379 6 114 72 1271 66 80
Cuts at: 3778 3817 4319 5061 5146 6217 1391
Size: 39 502 742 85 1071 2174
Fragments arranged by size:
2174 1271 1071 742 502 399 379 114
85 80 72 66 39 6
BspEI T'CCGG_A
Cuts at: 6663 6663
Size: 7000
BspHI T'CATG_A
Cuts at: 4249 4889 6619 4249
Size: 640 1730 4630
Fragments arranged by size:
4630 1730 640
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4355 844
Size: 3511 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2407 2974 4887 1394
Size: 429 584 567 1913 3507
Fragments arranged by size:
3507 1913 584 567 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3875 5495 5669 65
Size: 1183 2627 1620 174 1396
Fragments arranged by size:
2627 1620 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2027 2055 5644 5831 1700
Size: 110 217 28 3589 187 2869
Fragments arranged by size:
3589 2869 217 187 110 28
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7000
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7000
BssSI C'ACGA_G
Cuts at: 2657 3444 5060 6072 2657
Size: 787 1616 1012 3585
Fragments arranged by size:
3585 1616 1012 787
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3281 794
Size: 2487 4513
BstBI TT'CG_AA
Cuts at: 2007 5804 2007
Size: 3797 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7000
BstXI CCAn_nnnn'nTGG
Cuts at: 3025 3295 3025
Size: 270 6730
BstYI r'GATC_y
Cuts at: 1972 3739 4267 4330 4697 5082 5099 6551 6637
Size: 1767 528 63 367 385 17 1452 86
Cuts at: 6637 6649 6995 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1767 1452 528 385 367 346 86
63 17 12
Bsu36I CC'TnA_GG
Cuts at: 2040 3491 2040
Size: 1451 5549
BtgI C'CryG_G
Cuts at: 513 1065 2022 2241 2851 4190 513
Size: 552 957 219 610 1339 3323
Fragments arranged by size:
3323 1339 957 610 552 219
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6261
Fragments arranged by size:
6261 683 56
BtsI GCAGTG_nn'
Cuts at: 2844 3372 3909 4516 5310 5330 2844
Size: 528 537 607 794 20 4514
Fragments arranged by size:
4514 794 607 537 528 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7000
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6965
DraI TTT'AAA
Cuts at: 4304 4304
Size: 7000
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2771 6007 809
Size: 507 1455 3236 1802
Fragments arranged by size:
3236 1802 1455 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2025 2202 2238
Size: 54 1425 50 275 18 195 177 36
Cuts at: 2238 2364 2848 4447 5336 6965 8
Size: 126 484 1599 889 1629 43
Fragments arranged by size:
1629 1599 1425 889 484 275 195 177
126 54 50 43 36 18
EagI C'GGCC_G
Cuts at: 1537 1812 2202 2364 4447 1537
Size: 275 390 162 2083 4090
Fragments arranged by size:
4090 2083 390 275 162
EarI CTCTTCn'nnn_
Cuts at: 1353 2804 4157 4930 1353
Size: 1451 1353 773 3423
Fragments arranged by size:
3423 1451 1353 773
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2897 5587 5961 6107 387
Size: 1064 138 1308 2690 374 146 1280
Fragments arranged by size:
2690 1308 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2087 2534 2629 3220 3918 5063 5660
Size: 393 455 447 95 591 698 1145 597
Cuts at: 5660 6447 6715 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1145 787 698 597 591 455 447
393 268 95
EcoO109I rG'GnC_Cy
Cuts at: 2941 2952 3166 3702 2941
Size: 11 214 536 6239
Fragments arranged by size:
6239 536 214 11
EcoRI G'AATT_C
Cuts at: 4309 4687 4309
Size: 378 6622
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3848 3848
Size: 7000
FspI TGC'GCA
Cuts at: 2750 5506 2750
Size: 2756 4244
HaeII r_GCGC'y
Cuts at: 1678 2062 6147 1678
Size: 384 4085 2531
Fragments arranged by size:
4085 2531 384
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3269 3301 3440 3472 3790
Size: 32 135 32 1697 32 139 32 318
Cuts at: 3790 3822 3845 3877 5641 5673 5715 5747 1373
Size: 32 23 32 1764 32 42 32 2626
Fragments arranged by size:
2626 1764 1697 318 139 135 42 32
32 32 32 32 32 32 32 23
HincII GTy'rAC
Cuts at: 669 1657 1938 2899 4344 4590 669
Size: 988 281 961 1445 246 3079
Fragments arranged by size:
3079 1445 988 961 281 246
HindIII A'AGCT_T
Cuts at: 748 2826 748
Size: 2078 4922
HpaI GTT'AAC
Cuts at: 2899 4590 2899
Size: 1691 5309
KpnI G_GTAC'C
Cuts at: 4325 4325
Size: 7000
MfeI C'AATT_G
Cuts at: 4599 4599
Size: 7000
MluI A'CGCG_T
Cuts at: 6876 6876
Size: 7000
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3405 3963 4187 6114 6298 1305
Size: 2100 558 224 1927 184 2007
Fragments arranged by size:
2100 2007 1927 558 224 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2850 10
Size: 54 1425 343 1018 4160
Fragments arranged by size:
4160 1425 1018 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3023 4958 5317 5476 518
Size: 648 1857 1935 359 159 2042
Fragments arranged by size:
2042 1935 1857 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2389 2419 2673 4416 5093 6241 6486 1606
Size: 783 30 254 1743 677 1148 245 2120
Fragments arranged by size:
2120 1743 1148 783 677 254 245 30
NaeI GCC'GGC
Cuts at: 2029 2029
Size: 7000
NcoI C'CATG_G
Cuts at: 513 1065 2022 2851 4190 513
Size: 552 957 829 1339 3323
Fragments arranged by size:
3323 1339 957 829 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7000
NgoMIV G'CCGG_C
Cuts at: 2027 2027
Size: 7000
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7000
NotI GC'GGCC_GC
Cuts at: 4447 4447
Size: 7000
NspI r_CATG'y
Cuts at: 1132 1229 2749 2774 3052 3059 4358 5903 1132
Size: 97 1520 25 278 7 1299 1545 2229
Fragments arranged by size:
2229 1545 1520 1299 278 97 25 7
PasI CC'CwG_GG
Cuts at: 1348 3698 1348
Size: 2350 4650
PciI A'CATG_T
Cuts at: 2770 3055 5899 2770
Size: 285 2844 3871
Fragments arranged by size:
3871 2844 285
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7000
PfoI T'CCnGG_A
Cuts at: 2064 2064
Size: 7000
PmeI GTTT'AAAC
Cuts at: 4304 4304
Size: 7000
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5069 5101 6582 6614 5069
Size: 32 1481 32 5455
Fragments arranged by size:
5455 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3702 3702
Size: 7000
PshAI GACnn'nnGTC
Cuts at: 1613 2419 1613
Size: 806 6194
PsiI TTA'TAA
Cuts at: 4570 4570
Size: 7000
PstI C_TGCA'G
Cuts at: 830 2478 2672 4352 830
Size: 1648 194 1680 3478
Fragments arranged by size:
3478 1680 1648 194
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7000
PvuII CAG'CTG
Cuts at: 2389 2673 2389
Size: 284 6716
RsrII CG'GwC_CG
Cuts at: 2093 2093
Size: 7000
SalI G'TCGA_C
Cuts at: 1936 4342 1936
Size: 2406 4594
SanDI GG'GwC_CC
Cuts at: 3702 3702
Size: 7000
SapI GCTCTTCn'nnn_
Cuts at: 2804 2804
Size: 7000
SbfI CC_TGCA'GG
Cuts at: 4352 4352
Size: 7000
ScaI AGT'ACT
Cuts at: 5248 6960 5248
Size: 1712 5288
SfcI C'TryA_G
Cuts at: 826 1045 2474 2668 4348 5483 6164 6355 826
Size: 219 1429 194 1680 1135 681 191 1471
Fragments arranged by size:
1680 1471 1429 1135 681 219 194 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7000
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7000
SmaI CCC'GGG
Cuts at: 1872 2227 2355 4008 4327 1872
Size: 355 128 1653 319 4545
Fragments arranged by size:
4545 1653 355 319 128
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3004 4107 5104 6005 6267 820
Size: 197 926 1061 1103 997 901 262 1553
Fragments arranged by size:
1553 1103 1061 997 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4297 4791 493
Size: 3804 494 2702
Fragments arranged by size:
3804 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7000
SphI G_CATG'C
Cuts at: 1132 4358 1132
Size: 3226 3774
SspI AAT'ATT
Cuts at: 5 52 4924 5
Size: 47 4872 2081
Fragments arranged by size:
4872 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7000
StyI C'CwwG_G
Cuts at: 513 1065 2022 2851 3169 3611 3646 4190 4274
Size: 552 957 829 318 442 35 544 84
Cuts at: 4274 4439 513
Size: 165 3074
Fragments arranged by size:
3074 957 829 552 544 442 318 165
84 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2665 3055 3059 3320 5027 5044 5197
Size: 1255 1085 390 4 261 1707 17 153
Cuts at: 5197 5382 5827 325
Size: 185 445 1498
Fragments arranged by size:
1707 1498 1255 1085 445 390 261 185
153 17 4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5246 6958 96
Size: 274 80 33 51 1443 3269 1712 138
Fragments arranged by size:
3269 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2559 2591 6960 6992 2559
Size: 32 4369 32 2567
Fragments arranged by size:
4369 2567 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7000
XbaI T'CTAG_A
Cuts at: 4022 4336 4022
Size: 314 6686
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4272 4272
Size: 7000
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7000
XmaI C'CCGG_G
Cuts at: 1870 2225 2353 4006 4325 1870
Size: 355 128 1653 319 4545
Fragments arranged by size:
4545 1653 355 319 128
XmnI GAAnn'nnTTC
Cuts at: 2547 2684 5129 2547
Size: 137 2445 4418
Fragments arranged by size:
4418 2445 137
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4808 276
Size: 53 83 186 936 3274 2468
Fragments arranged by size:
3274 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII RsrII SalI SanDI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BaeI BbeI BfrBI BsiWI
BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI
KasI NarI NruI NsiI PacI PmlI PspOMI PspXI
PsrI SacI SacII SexAI SfiI SfoI SrfI SwaI