Restriction map for
pFN21ASDA1067
(Circular) (Six-base) MAPSORT of: pFN21ASDA1067.seq Check: 9213 from: 1 to: 6781
pFN21ASDA1067 6781 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2601 4024 2601
Size: 1423 5358
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4591 278
Size: 53 83 186 936 3055 2468
Fragments arranged by size:
3055 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2676 4124 1937
Size: 739 1448 4594
Fragments arranged by size:
4594 1448 739
Acc65I G'GTAC_C
Cuts at: 4102 4102
Size: 6781
AclI AA'CG_TT
Cuts at: 1563 4908 5281 1563
Size: 3345 373 3063
Fragments arranged by size:
3345 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2096 2744 4844 6228 6496 1632
Size: 464 648 2100 1384 268 1917
Fragments arranged by size:
2100 1917 1384 648 464 268
AfeI AGC'GCT
Cuts at: 3294 3686 3294
Size: 392 6389
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6584
AflIII A'CryG_T
Cuts at: 1203 5680 6657 1203
Size: 4477 977 1327
Fragments arranged by size:
4477 1327 977
AgeI A'CCGG_T
Cuts at: 5612 5612
Size: 6781
AhdI GACnn_n'nnGTC
Cuts at: 5510 5510
Size: 6781
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6781
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3515 1184
Size: 2331 4450
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2011 2043 2011
Size: 32 6749
AlwNI CAG_nnn'CTG
Cuts at: 2239 3013 4524 6096 2239
Size: 774 1511 1572 2924
Fragments arranged by size:
2924 1572 1511 774
ApaI G_GGCC'C
Cuts at: 2336 2336
Size: 6781
ApaLI G'TGCA_C
Cuts at: 2210 4838 5994 2210
Size: 2628 1156 2997
Fragments arranged by size:
2997 2628 1156
ApoI r'AATT_y
Cuts at: 1493 2480 4090 4316 4468 6577 1493
Size: 987 1610 226 152 2109 1697
Fragments arranged by size:
2109 1697 1610 987 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5335 160
Size: 872 4303 1606
Fragments arranged by size:
4303 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6781
AvaI C'yCGr_G
Cuts at: 1870 1943 2597 3029 4106 1870
Size: 73 654 432 1077 4545
Fragments arranged by size:
4545 1077 654 432 73
BamHI G'GATC_C
Cuts at: 4111 4478 4111
Size: 367 6414
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3284 3359 3431 4102 5557
Size: 325 217 627 1497 75 72 671 1455
Cuts at: 5557 618
Size: 1842
Fragments arranged by size:
1842 1497 1455 671 627 325 217 75
72
BanII G_rGCy'C
Cuts at: 1391 2336 2903 3375 4100 1391
Size: 945 567 472 725 4072
Fragments arranged by size:
4072 945 725 567 472
BbeI G_GCGC'C
Cuts at: 3435 3435
Size: 6781
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2264 3053 3157 3680 3941 928
Size: 971 365 789 104 523 261 3768
Fragments arranged by size:
3768 971 789 523 365 261 104
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4052 4086 4972 5006 1903
Size: 34 2115 34 886 34 3678
Fragments arranged by size:
3678 2115 886 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4673 5883 4673
Size: 1210 5571
BclI T'GATC_A
Cuts at: 1548 2541 1548
Size: 993 5788
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3125 3440 5392
Size: 107 122 71 631 605 1453 315 1952
Cuts at: 5392 136
Size: 1525
Fragments arranged by size:
1952 1525 1453 631 605 315 122 107
71
BglII A'GATC_T
Cuts at: 6776 6776
Size: 6781
BlpI GC'TnA_GC
Cuts at: 3503 3551 3681 4198 3503
Size: 48 130 517 6086
Fragments arranged by size:
6086 517 130 48
Bme1580I G_kGCm'C
Cuts at: 1790 2214 2336 3227 3990 4842 5998 1790
Size: 424 122 891 763 852 1156 2573
Fragments arranged by size:
2573 1156 891 852 763 424 122
BmgBI CAC'GTC
Cuts at: 1318 2578 2944 1318
Size: 1260 366 5155
Fragments arranged by size:
5155 1260 366
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4512 5470 442
Size: 416 53 83 399 24 3095 958 1753
Fragments arranged by size:
3095 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6781
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3475 3815 3475
Size: 340 6441
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2138 2210 2492 2582 2594 2606 2677 3203
Size: 899 72 282 90 12 12 71 526
Cuts at: 3203 3317 3653 5441 1239
Size: 114 336 1788 2579
Fragments arranged by size:
2579 1788 899 526 336 282 114 90
72 71 12 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3170 3608 4906 5771 6069 3170
Size: 438 1298 865 298 3882
Fragments arranged by size:
3882 1298 865 438 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2942 3269 3446 3944 5444 882
Size: 617 1443 327 177 498 1500 2219
Fragments arranged by size:
2219 1500 1443 617 498 327 177
BsaAI yAC'GTr
Cuts at: 493 1204 4078 4572 493
Size: 711 2874 494 2702
Fragments arranged by size:
2874 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 6775 6775
Size: 6781
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3432 4588 4970 5605
Size: 53 83 186 936 1899 1156 382 635
Cuts at: 5605 275
Size: 1451
Fragments arranged by size:
1899 1451 1156 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5214 5612 5886 6033 6444 5214
Size: 398 274 147 411 5551
Fragments arranged by size:
5551 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2081 2111 3131 3161 1178
Size: 30 165 30 678 30 1020 30 4798
Fragments arranged by size:
4798 1020 678 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2099 2102 1186
Size: 913 3 5865
Fragments arranged by size:
5865 913 3
BseYI C'CCAG_C
Cuts at: 2871 3526 3577 5984 6480 2871
Size: 655 51 2407 496 3172
Fragments arranged by size:
3172 2407 655 496 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3584 1163
Size: 573 1848 4360
Fragments arranged by size:
4360 1848 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4054 4231 4992 6020 1540
Size: 275 186 2053 177 761 1028 2301
Fragments arranged by size:
2301 2053 1028 761 275 186 177
BsiHKAI G_wGCw'C
Cuts at: 2214 2529 2592 2610 3036 3159 3219 3375 3702
Size: 315 63 18 426 123 60 156 327
Cuts at: 3702 4842 4927 5998 2214
Size: 1140 85 1071 2997
Fragments arranged by size:
2997 1140 1071 426 327 315 156 123
85 63 60 18
BsmI GAATG_Cn'
Cuts at: 4292 4385 4292
Size: 93 6688
BsmBI CGTCTCn'nnnn_
Cuts at: 2521 3416 2521
Size: 895 5886
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2214 2336 2529 2592 2610 2903 3036
Size: 399 424 122 193 63 18 293 133
Cuts at: 3036 3159 3219 3227 3375 3702 3990 4100 4842
Size: 123 60 8 148 327 288 110 742
Cuts at: 4842 4927 5998 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 424 399 327 293 288
193 148 133 123 122 110 85 63
60 18 8
BspEI T'CCGG_A
Cuts at: 6444 6444
Size: 6781
BspHI T'CATG_A
Cuts at: 2667 4670 6400 2667
Size: 2003 1730 3048
Fragments arranged by size:
3048 2003 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2601 3543 4024 4136 844
Size: 1757 942 481 112 3489
Fragments arranged by size:
3489 1757 942 481 112
BsrBI CCG'CTC
Cuts at: 1394 1823 2048 2709 3814 4668 1394
Size: 429 225 661 1105 854 3507
Fragments arranged by size:
3507 1105 854 661 429 225
BsrDI GCAATG_nn'
Cuts at: 65 1248 3388 5276 5450 65
Size: 1183 2140 1888 174 1396
Fragments arranged by size:
2140 1888 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5425 5612 1700
Size: 110 3615 187 2869
Fragments arranged by size:
3615 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6781
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6781
BssSI C'ACGA_G
Cuts at: 3368 4841 5853 3368
Size: 1473 1012 4296
Fragments arranged by size:
4296 1473 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2239 794
Size: 1445 5336
BstBI TT'CG_AA
Cuts at: 2007 5585 2007
Size: 3578 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6781
BstXI CCAn_nnnn'nTGG
Cuts at: 2701 3295 2701
Size: 594 6187
BstYI r'GATC_y
Cuts at: 1972 2536 3714 4111 4478 4863 4880 6332 6418
Size: 564 1178 397 367 385 17 1452 86
Cuts at: 6418 6430 6776 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1178 564 397 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3150 3270 513
Size: 552 2085 120 4024
Fragments arranged by size:
4024 2085 552 120
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3742 487
Size: 683 56 2516 3526
Fragments arranged by size:
3526 2516 683 56
BtsI GCAGTG_nn'
Cuts at: 2394 2965 3669 4297 5091 5111 2394
Size: 571 704 628 794 20 4064
Fragments arranged by size:
4064 794 704 628 571 20
ClaI AT'CG_AT
Cuts at: 1554 4050 1554
Size: 2496 4285
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6746
DraI TTT'AAA
Cuts at: 4085 4085
Size: 6781
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5788 809
Size: 507 4472 1802
Fragments arranged by size:
4472 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2301 2370 2650
Size: 54 1425 50 275 18 471 69 280
Cuts at: 2650 2825 3015 3465 4228 5117 6746 8
Size: 175 190 450 763 889 1629 43
Fragments arranged by size:
1629 1425 889 763 471 450 280 275
190 175 69 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4228 1537
Size: 275 2416 4090
Fragments arranged by size:
4090 2416 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2117 2346 3398 3466 4711 1353
Size: 764 229 1052 68 1245 3423
Fragments arranged by size:
3423 1245 1052 764 229 68
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5368 5742 5888 387
Size: 1064 138 3779 374 146 1280
Fragments arranged by size:
3779 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3373 3373
Size: 6781
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2096 2138 2210 2492 2582 2594 2606
Size: 393 464 42 72 282 90 12 12
Cuts at: 2606 2677 2744 3203 3317 3653 4844 5441 6228
Size: 71 67 459 114 336 1191 597 787
Cuts at: 6228 6496 1239
Size: 268 1524
Fragments arranged by size:
1524 1191 787 597 464 459 393 336
282 268 114 90 72 71 67 42
12 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2919 3065 2919
Size: 146 6635
EcoO109I rG'GnC_Cy
Cuts at: 2332 2333 2467 3574 3960 2332
Size: 1 134 1107 386 5153
Fragments arranged by size:
5153 1107 386 134 1
EcoRI G'AATT_C
Cuts at: 4090 4468 4090
Size: 378 6403
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3067 3067
Size: 6781
FspI TGC'GCA
Cuts at: 5287 5287
Size: 6781
HaeII r_GCGC'y
Cuts at: 1678 3296 3435 3591 3688 5928 1678
Size: 1618 139 156 97 2240 2531
Fragments arranged by size:
2531 2240 1618 156 139 97
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2081 2113 2212 2244 5422
Size: 32 135 32 509 32 99 32 3178
Cuts at: 5422 5454 5496 5528 1373
Size: 32 42 32 2626
Fragments arranged by size:
3178 2626 509 135 99 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4125 4371 669
Size: 988 281 2187 246 3079
Fragments arranged by size:
3079 2187 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6781
HpaI GTT'AAC
Cuts at: 4371 4371
Size: 6781
KasI G'GCGC_C
Cuts at: 3431 3431
Size: 6781
KpnI G_GTAC'C
Cuts at: 4106 4106
Size: 6781
MfeI C'AATT_G
Cuts at: 4380 4380
Size: 6781
MluI A'CGCG_T
Cuts at: 6657 6657
Size: 6781
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5895 6079 1305
Size: 4590 184 2007
Fragments arranged by size:
4590 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2303 2372 10
Size: 54 1425 343 471 69 4419
Fragments arranged by size:
4419 1425 471 343 69 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2207 3269 4739 5098 5257 518
Size: 648 1041 1062 1470 359 159 2042
Fragments arranged by size:
2042 1470 1062 1041 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2236 2634 2701 3010 3655 3735 4197 4874
Size: 630 398 67 309 645 80 462 677
Cuts at: 4874 6022 6267 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 645 630 462 398 309
245 80 67
NarI GG'CG_CC
Cuts at: 3432 3432
Size: 6781
NcoI C'CATG_G
Cuts at: 513 1065 3270 513
Size: 552 2205 4024
Fragments arranged by size:
4024 2205 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6781
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6781
NotI GC'GGCC_GC
Cuts at: 4228 4228
Size: 6781
NspI r_CATG'y
Cuts at: 1132 1229 2938 3349 4139 5684 1132
Size: 97 1709 411 790 1545 2229
Fragments arranged by size:
2229 1709 1545 790 411 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6781
PciI A'CATG_T
Cuts at: 5680 5680
Size: 6781
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3013 3658 1108
Size: 1905 645 4231
Fragments arranged by size:
4231 1905 645
PfoI T'CCnGG_A
Cuts at: 2400 2610 3417 3801 2400
Size: 210 807 384 5380
Fragments arranged by size:
5380 807 384 210
PmeI GTTT'AAAC
Cuts at: 4085 4085
Size: 6781
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4850 4882 6363 6395 4850
Size: 32 1481 32 5236
Fragments arranged by size:
5236 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3574 3574
Size: 6781
PshAI GACnn'nnGTC
Cuts at: 1613 3941 1613
Size: 2328 4453
PsiI TTA'TAA
Cuts at: 4351 4351
Size: 6781
PspOMI G'GGCC_C
Cuts at: 2332 2332
Size: 6781
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2663 2695 2663
Size: 32 6749
PstI C_TGCA'G
Cuts at: 830 2235 2244 3402 3540 4133 830
Size: 1405 9 1158 138 593 3478
Fragments arranged by size:
3478 1405 1158 593 138 9
PvuI CG_AT'CG
Cuts at: 2001 4054 2001
Size: 2053 4728
PvuII CAG'CTG
Cuts at: 2701 3010 3655 3735 2701
Size: 309 645 80 5747
Fragments arranged by size:
5747 645 309 80
SacI G_AGCT'C
Cuts at: 3375 3375
Size: 6781
SalI G'TCGA_C
Cuts at: 1936 4123 1936
Size: 2187 4594
SapI GCTCTTCn'nnn_
Cuts at: 2117 3466 2117
Size: 1349 5432
SbfI CC_TGCA'GG
Cuts at: 4133 4133
Size: 6781
ScaI AGT'ACT
Cuts at: 5029 6741 5029
Size: 1712 5069
SexAI A'CCwGG_T
Cuts at: 3210 3210
Size: 6781
SfcI C'TryA_G
Cuts at: 826 1045 2231 2240 3398 3475 3536 3968 4129
Size: 219 1186 9 1158 77 61 432 161
Cuts at: 4129 5264 5945 6136 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1186 1158 1135 681 432 219 191
161 77 61 9
SfoI GGC'GCC
Cuts at: 3433 3433
Size: 6781
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6781
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6781
SmaI CCC'GGG
Cuts at: 1872 4108 1872
Size: 2236 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3185 3623 4885 5786 6048 820
Size: 197 926 1242 438 1262 901 262 1553
Fragments arranged by size:
1553 1262 1242 926 901 438 262 197
SnaBI TAC'GTA
Cuts at: 493 4078 4572 493
Size: 3585 494 2702
Fragments arranged by size:
3585 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6781
SphI G_CATG'C
Cuts at: 1132 4139 1132
Size: 3007 3774
SspI AAT'ATT
Cuts at: 5 52 4705 5
Size: 47 4653 2081
Fragments arranged by size:
4653 2081 47
StuI AGG'CCT
Cuts at: 1517 3884 1517
Size: 2367 4414
StyI C'CwwG_G
Cuts at: 513 1065 3270 3561 4220 513
Size: 552 2205 291 659 3074
Fragments arranged by size:
3074 2205 659 552 291
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2966 4808 4825 4978 5163 5608 325
Size: 1255 1386 1842 17 153 185 445 1498
Fragments arranged by size:
1842 1498 1386 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3339 4011 5027
Size: 274 80 33 51 1443 1362 672 1016
Cuts at: 5027 6739 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1362 1016 672 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3131 3163 6741 6773 3131
Size: 32 3578 32 3139
Fragments arranged by size:
3578 3139 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2095 2263 2279 1316
Size: 779 168 16 5818
Fragments arranged by size:
5818 779 168 16
XbaI T'CTAG_A
Cuts at: 2881 4117 2881
Size: 1236 5545
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2344 2344
Size: 6781
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6781
XmaI C'CCGG_G
Cuts at: 1870 4106 1870
Size: 2236 4545
XmnI GAAnn'nnTTC
Cuts at: 3050 4910 3050
Size: 1860 4921
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4589 276
Size: 53 83 186 936 3055 2468
Fragments arranged by size:
3055 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstZ17I
Bsu36I DraIII EcoRV FseI FspAI NaeI NgoMIV NruI
NsiI PacI PmlI PspXI RsrII SacII SanDI SfiI
SrfI SwaI