Restriction map for
pFN21ASDA1066
(Circular) (Six-base) MAPSORT of: pFN21ASDA1066.seq Check: 5502 from: 1 to: 8737
pFN21ASDA1066 8737 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 6001 6001
Size: 8737
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6547 278
Size: 53 83 186 936 5011 2468
Fragments arranged by size:
5011 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3182 3366 3731 6080 1937
Size: 1245 184 365 2349 4594
Fragments arranged by size:
4594 2349 1245 365 184
Acc65I G'GTAC_C
Cuts at: 6058 6058
Size: 8737
AclI AA'CG_TT
Cuts at: 1563 6864 7237 1563
Size: 5301 373 3063
Fragments arranged by size:
5301 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2456 3733 3745 3829 5036 6800 8184 8452
Size: 824 1277 12 84 1207 1764 1384 268
Cuts at: 8452 1632
Size: 1917
Fragments arranged by size:
1917 1764 1384 1277 1207 824 268 84
12
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8540
AflIII A'CryG_T
Cuts at: 1203 2649 3078 4278 5637 7636 8613 1203
Size: 1446 429 1200 1359 1999 977 1327
Fragments arranged by size:
1999 1446 1359 1327 1200 977 429
AgeI A'CCGG_T
Cuts at: 7568 7568
Size: 8737
AhdI GACnn_n'nnGTC
Cuts at: 3034 3373 3925 7466 3034
Size: 339 552 3541 4305
Fragments arranged by size:
4305 3541 552 339
AleI CACnn'nnGTG
Cuts at: 1166 2727 3596 4409 4565 4628 4721 5100 1166
Size: 1561 869 813 156 63 93 379 4803
Fragments arranged by size:
4803 1561 869 813 379 156 93 63
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2717 5748 1184
Size: 1533 3031 4173
Fragments arranged by size:
4173 3031 1533
AlwNI CAG_nnn'CTG
Cuts at: 2905 3194 3313 4733 4856 5543 5569 5857 6480
Size: 289 119 1420 123 687 26 288 623
Cuts at: 6480 8052 2905
Size: 1572 3590
Fragments arranged by size:
3590 1572 1420 687 623 289 288 123
119 26
ApaI G_GGCC'C
Cuts at: 4740 5816 4740
Size: 1076 7661
ApaLI G'TGCA_C
Cuts at: 2679 4967 5222 6794 7950 2679
Size: 2288 255 1572 1156 3466
Fragments arranged by size:
3466 2288 1572 1156 255
ApoI r'AATT_y
Cuts at: 1493 2342 3902 6046 6272 6424 8533 1493
Size: 849 1560 2144 226 152 2109 1697
Fragments arranged by size:
2144 2109 1697 1560 849 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7291 160
Size: 872 6259 1606
Fragments arranged by size:
6259 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8737
AvaI C'yCGr_G
Cuts at: 1870 1943 2946 3696 4286 4490 4531 4614 4769
Size: 73 1003 750 590 204 41 83 155
Cuts at: 4769 5621 6026 6062 1870
Size: 852 405 36 4545
Fragments arranged by size:
4545 1003 852 750 590 405 204 155
83 73 41 36
AvrII C'CTAG_G
Cuts at: 3232 3232
Size: 8737
BamHI G'GATC_C
Cuts at: 4344 6067 6434 4344
Size: 1723 367 6647
Fragments arranged by size:
6647 1723 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2721 2831 4093 4723 5501
Size: 325 217 627 934 110 1262 630 778
Cuts at: 5501 5612 5724 5755 6058 7513 618
Size: 111 112 31 303 1455 1842
Fragments arranged by size:
1842 1455 1262 934 778 630 627 325
303 217 112 111 110 31
BanII G_rGCy'C
Cuts at: 1391 2126 2187 2927 4266 4311 4740 4890 4948
Size: 735 61 740 1339 45 429 150 58
Cuts at: 4948 5409 5577 5816 6056 1391
Size: 461 168 239 240 4072
Fragments arranged by size:
4072 1339 740 735 461 429 240 239
168 150 61 58 45
BbeI G_GCGC'C
Cuts at: 5616 5616
Size: 8737
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4783 928
Size: 971 2884 4882
Fragments arranged by size:
4882 2884 971
BbvCI CC'TCA_GC
Cuts at: 3395 3395
Size: 8737
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6928 6962 1903
Size: 34 4991 34 3678
Fragments arranged by size:
4991 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6629 7839 6629
Size: 1210 7527
BclI T'GATC_A
Cuts at: 1548 3684 4380 1548
Size: 2136 696 5905
Fragments arranged by size:
5905 2136 696
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2121 4712 7348
Size: 107 122 71 631 605 449 2591 2636
Cuts at: 7348 136
Size: 1525
Fragments arranged by size:
2636 2591 1525 631 605 449 122 107
71
BglII A'GATC_T
Cuts at: 8732 8732
Size: 8737
BlpI GC'TnA_GC
Cuts at: 2231 3417 5030 5876 6154 2231
Size: 1186 1613 846 278 4814
Fragments arranged by size:
4814 1613 1186 846 278
Bme1580I G_kGCm'C
Cuts at: 1790 2683 2865 2947 3875 4406 4740 4971 5226
Size: 893 182 82 928 531 334 231 255
Cuts at: 5226 5504 5727 5816 6798 7954 1790
Size: 278 223 89 982 1156 2573
Fragments arranged by size:
2573 1156 982 928 893 531 334 278
255 231 223 182 89 82
BmgBI CAC'GTC
Cuts at: 1318 3927 4281 4516 4789 5227 5395 1318
Size: 2609 354 235 273 438 168 4660
Fragments arranged by size:
4660 2609 438 354 273 235 168
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2293 4622 4684
Size: 416 53 83 399 24 876 2329 62
Cuts at: 4684 6468 7426 442
Size: 1784 958 1753
Fragments arranged by size:
2329 1784 1753 958 876 416 399 83
62 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8737
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5582 5582
Size: 8737
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2231 2282 2450 2740 3757 4039 4315 4438
Size: 992 51 168 290 1017 282 276 123
Cuts at: 4438 7397 1239
Size: 2959 2579
Fragments arranged by size:
2959 2579 1017 992 290 282 276 168
123 51
Bpu10I CC'TnA_GC
Cuts at: 3395 4166 3395
Size: 771 7966
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6862 7727 8025 6862
Size: 865 298 7574
Fragments arranged by size:
7574 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3083 7400 882
Size: 617 1584 4317 2219
Fragments arranged by size:
4317 2219 1584 617
BsaAI yAC'GTr
Cuts at: 493 1204 2548 2678 3982 4441 5638 6034 6528
Size: 711 1344 130 1304 459 1197 396 494
Cuts at: 6528 493
Size: 2702
Fragments arranged by size:
2702 1344 1304 1197 711 494 459 396
130
BsaBI GATnn'nnATC
Cuts at: 2030 8731 2030
Size: 6701 2036
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4158 4323 4389 5268
Size: 53 83 186 936 2625 165 66 879
Cuts at: 5268 5613 6544 6926 7561 275
Size: 345 931 382 635 1451
Fragments arranged by size:
2625 1451 936 931 879 635 382 345
186 165 83 66 53
BsaWI w'CCGG_w
Cuts at: 7170 7568 7842 7989 8400 7170
Size: 398 274 147 411 7507
Fragments arranged by size:
7507 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2066 2093 2096 2123 2468
Size: 30 165 30 663 27 3 27 345
Cuts at: 2468 2498 3146 3176 4313 4343 4595 4625 1178
Size: 30 648 30 1137 30 252 30 5290
Fragments arranged by size:
5290 1137 663 648 345 252 165 30
30 30 30 30 30 27 27 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2183 2351 2489 4600 4715 5951 1186
Size: 997 168 138 2111 115 1236 3972
Fragments arranged by size:
3972 2111 1236 997 168 138 115
BseYI C'CCAG_C
Cuts at: 2819 3576 4196 4739 4801 4827 4889 4939 5440
Size: 757 620 543 62 26 62 50 501
Cuts at: 5440 5706 5803 7940 8436 2819
Size: 266 97 2137 496 3120
Fragments arranged by size:
3120 2137 757 620 543 501 496 266
97 62 62 50 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2498 3907 4908 4985 5537 1163
Size: 573 762 1409 1001 77 552 4363
Fragments arranged by size:
4363 1409 1001 762 573 552 77
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2811 2871 4000 4246 5194 6187
Size: 275 186 810 60 1129 246 948 993
Cuts at: 6187 6948 7976 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1129 1028 993 948 810 761 275
246 186 60
BsiHKAI G_wGCw'C
Cuts at: 2187 2232 2250 2406 2556 2683 3618 3705 3981
Size: 45 18 156 150 127 935 87 276
Cuts at: 3981 4311 4788 4971 5226 6798 6883 7954 2187
Size: 330 477 183 255 1572 85 1071 2970
Fragments arranged by size:
2970 1572 1071 935 477 330 276 255
183 156 150 127 87 85 45 18
BsiWI C'GTAC_G
Cuts at: 5455 5455
Size: 8737
BsmI GAATG_Cn'
Cuts at: 6248 6341 6248
Size: 93 8644
BsmBI CGTCTCn'nnnn_
Cuts at: 4248 4287 5907 5924 4248
Size: 39 1620 17 7061
Fragments arranged by size:
7061 1620 39 17
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2126 2187 2232 2250 2406 2556 2683
Size: 399 336 61 45 18 156 150 127
Cuts at: 2683 2865 2927 2947 3618 3705 3875 3981 4266
Size: 182 62 20 671 87 170 106 285
Cuts at: 4266 4311 4406 4740 4788 4890 4948 4971 5226
Size: 45 95 334 48 102 58 23 255
Cuts at: 5226 5409 5504 5577 5727 5816 6056 6798 6883
Size: 183 95 73 150 89 240 742 85
Cuts at: 6883 7954 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 671 399 336 334 285
255 240 183 182 170 156 150 150
127 106 102 95 95 89 87 85
73 62 61 58 48 45 45 23
20 18
BspEI T'CCGG_A
Cuts at: 8400 8400
Size: 8737
BspHI T'CATG_A
Cuts at: 6626 8356 6626
Size: 1730 7007
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2569 4247 6001 6092 844
Size: 1725 1678 1754 91 3489
Fragments arranged by size:
3489 1754 1725 1678 91
BsrBI CCG'CTC
Cuts at: 1394 1823 2104 2479 3625 3643 4170 5880 6624
Size: 429 281 375 1146 18 527 1710 744
Cuts at: 6624 1394
Size: 3507
Fragments arranged by size:
3507 1710 1146 744 527 429 375 281
18
BsrDI GCAATG_nn'
Cuts at: 65 1248 4760 7232 7406 65
Size: 1183 3512 2472 174 1396
Fragments arranged by size:
3512 2472 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3839 3891 4090 4096 4553 5487 5504
Size: 110 2029 52 199 6 457 934 17
Cuts at: 5504 7381 7568 1700
Size: 1877 187 2869
Fragments arranged by size:
2869 2029 1877 934 457 199 187 110
52 17 6
BsrGI T'GTAC_A
Cuts at: 96 3548 96
Size: 3452 5285
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8737
BssSI C'ACGA_G
Cuts at: 2249 2699 6797 7809 2249
Size: 450 4098 1012 3177
Fragments arranged by size:
4098 3177 1012 450
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3391 794
Size: 2597 6140
BstBI TT'CG_AA
Cuts at: 2007 7541 2007
Size: 5534 3203
BstEII G'GTnAC_C
Cuts at: 1184 2983 1184
Size: 1799 6938
BstXI CCAn_nnnn'nTGG
Cuts at: 4375 4939 5341 5642 4375
Size: 564 402 301 7470
Fragments arranged by size:
7470 564 402 301
BstYI r'GATC_y
Cuts at: 1972 2031 4344 5119 6067 6434 6819 6836 8288
Size: 59 2313 775 948 367 385 17 1452
Cuts at: 8288 8374 8386 8732 1972
Size: 86 12 346 1977
Fragments arranged by size:
2313 1977 1452 948 775 385 367 346
86 59 17 12
Bsu36I CC'TnA_GG
Cuts at: 5354 5354
Size: 8737
BtgI C'CryG_G
Cuts at: 513 1065 3570 4152 4464 4595 4716 4902 5098
Size: 552 2505 582 312 131 121 186 196
Cuts at: 5098 5276 5694 5719 513
Size: 178 418 25 3531
Fragments arranged by size:
3531 2505 582 552 418 312 196 186
178 131 121 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3267 3481 4648 5331 5661 5745
Size: 683 56 2041 214 1167 683 330 84
Cuts at: 5745 487
Size: 3479
Fragments arranged by size:
3479 2041 1167 683 683 330 214 84
56
BtsI GCAGTG_nn'
Cuts at: 2155 3031 3834 4924 5662 5794 6253 7047 7067
Size: 876 803 1090 738 132 459 794 20
Cuts at: 7067 2155
Size: 3825
Fragments arranged by size:
3825 1090 876 803 794 738 459 132
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8737
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8702
DraI TTT'AAA
Cuts at: 6041 6041
Size: 8737
DraIII CAC_nnn'GTG
Cuts at: 2254 4935 2254
Size: 2681 6056
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3190 7744 809
Size: 507 1874 4554 1802
Fragments arranged by size:
4554 1874 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2737 2808 2868
Size: 54 1425 50 275 18 907 71 60
Cuts at: 2868 3786 3997 4467 4551 4656 4874 4980 5085
Size: 918 211 470 84 105 218 106 105
Cuts at: 5085 5110 5667 5772 6184 7073 8702 8
Size: 25 557 105 412 889 1629 43
Fragments arranged by size:
1629 1425 918 907 889 557 470 412
275 218 211 106 105 105 105 84
71 60 54 50 43 25 18
EagI C'GGCC_G
Cuts at: 1537 1812 2808 2868 3997 6184 1537
Size: 275 996 60 1129 2187 4090
Fragments arranged by size:
4090 2187 1129 996 275 60
EarI CTCTTCn'nnn_
Cuts at: 1353 3456 3513 3764 3772 6667 1353
Size: 2103 57 251 8 2895 3423
Fragments arranged by size:
3423 2895 2103 251 57 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2348 2635 3539 3922 5087 5327
Size: 1064 138 759 287 904 383 1165 240
Cuts at: 5327 7324 7698 7844 387
Size: 1997 374 146 1280
Fragments arranged by size:
1997 1280 1165 1064 904 759 383 374
287 240 146 138
EcoICRI GAG'CTC
Cuts at: 2185 4309 2185
Size: 2124 6613
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2231 2282 2450 2456 2740 3733 3745
Size: 393 599 51 168 6 284 993 12
Cuts at: 3745 3757 3829 4039 4315 4438 5036 6800 7397
Size: 12 72 210 276 123 598 1764 597
Cuts at: 7397 8184 8452 1239
Size: 787 268 1524
Fragments arranged by size:
1764 1524 993 787 599 598 597 393
284 276 268 210 168 123 72 51
12 12 6
EcoO109I rG'GnC_Cy
Cuts at: 4126 4528 4897 5079 5812 5813 4126
Size: 402 369 182 733 1 7050
Fragments arranged by size:
7050 733 402 369 182 1
EcoRI G'AATT_C
Cuts at: 3902 6046 6424 3902
Size: 2144 378 6215
Fragments arranged by size:
6215 2144 378
FspI TGC'GCA
Cuts at: 2324 2999 7243 2324
Size: 675 4244 3818
Fragments arranged by size:
4244 3818 675
HaeII r_GCGC'y
Cuts at: 1678 2149 2321 2635 3583 3796 3856 4220 4275
Size: 471 172 314 948 213 60 364 55
Cuts at: 4275 5616 7884 1678
Size: 1341 2268 2531
Fragments arranged by size:
2531 2268 1341 948 471 364 314 213
172 60 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2066 2093 2098 2125 2468
Size: 32 135 32 494 27 5 27 343
Cuts at: 2468 2500 2768 2800 3020 3052 3359 3391 3692
Size: 32 268 32 220 32 307 32 301
Cuts at: 3692 3724 3911 3943 4313 4345 5116 5148 5505
Size: 32 187 32 370 32 771 32 357
Cuts at: 5505 5537 7378 7410 7452 7484 1373
Size: 32 1841 32 42 32 2626
Fragments arranged by size:
2626 1841 771 494 370 357 343 307
301 268 220 187 135 42 32 32
32 32 32 32 32 32 32 32
32 32 32 27 27 5
HincII GTy'rAC
Cuts at: 669 1657 1938 3183 3367 3732 4162 4681 6081
Size: 988 281 1245 184 365 430 519 1400
Cuts at: 6081 6327 669
Size: 246 3079
Fragments arranged by size:
3079 1400 1245 988 519 430 365 281
246 184
HindIII A'AGCT_T
Cuts at: 748 3141 748
Size: 2393 6344
HpaI GTT'AAC
Cuts at: 6327 6327
Size: 8737
KasI G'GCGC_C
Cuts at: 5612 5612
Size: 8737
KpnI G_GTAC'C
Cuts at: 6062 6062
Size: 8737
MfeI C'AATT_G
Cuts at: 6336 6336
Size: 8737
MluI A'CGCG_T
Cuts at: 8613 8613
Size: 8737
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2079 2451 2466 2563 2865 3387 7851 8035
Size: 774 372 15 97 302 522 4464 184
Cuts at: 8035 1305
Size: 2007
Fragments arranged by size:
4464 2007 774 522 372 302 184 97
15
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4658 4982 5087 5112 5774
Size: 54 1425 343 2826 324 105 25 662
Cuts at: 5774 10
Size: 2973
Fragments arranged by size:
2973 2826 1425 662 343 324 105 54
25
MslI CAynn'nnrTG
Cuts at: 518 1166 2531 2727 3596 4076 4409 4565 4628
Size: 648 1365 196 869 480 333 156 63
Cuts at: 4628 4721 5100 5636 5640 5730 6002 6695 7054
Size: 93 379 536 4 90 272 693 359
Cuts at: 7054 7213 518
Size: 159 2042
Fragments arranged by size:
2042 1365 869 693 648 536 480 379
359 333 272 196 159 156 93 90
63 4
MspA1I CmG'CkG
Cuts at: 1606 2194 2290 2428 2521 2743 3373 4045 4154
Size: 588 96 138 93 222 630 672 109
Cuts at: 4154 4466 4584 4597 4893 5172 5278 5302 5825
Size: 312 118 13 296 279 106 24 523
Cuts at: 5825 6153 6830 7978 8223 1606
Size: 328 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 672 630 588 523 328
312 296 279 245 222 138 118 109
106 96 93 24 13
NaeI GCC'GGC
Cuts at: 3841 3893 5489 3841
Size: 52 1596 7089
Fragments arranged by size:
7089 1596 52
NarI GG'CG_CC
Cuts at: 5613 5613
Size: 8737
NcoI C'CATG_G
Cuts at: 513 1065 3570 5694 5719 513
Size: 552 2505 2124 25 3531
Fragments arranged by size:
3531 2505 2124 552 25
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8737
NgoMIV G'CCGG_C
Cuts at: 3839 3891 5487 3839
Size: 52 1596 7089
Fragments arranged by size:
7089 1596 52
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8737
NotI GC'GGCC_GC
Cuts at: 2808 6184 2808
Size: 3376 5361
NspI r_CATG'y
Cuts at: 1132 1229 3082 5045 6095 7640 1132
Size: 97 1853 1963 1050 1545 2229
Fragments arranged by size:
2229 1963 1853 1545 1050 97
PasI CC'CwG_GG
Cuts at: 1348 2905 3124 4733 5816 1348
Size: 1557 219 1609 1083 4269
Fragments arranged by size:
4269 1609 1557 1083 219
PciI A'CATG_T
Cuts at: 3078 7636 3078
Size: 4558 4179
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4733 4935 5569 1108
Size: 3625 202 634 4276
Fragments arranged by size:
4276 3625 634 202
PfoI T'CCnGG_A
Cuts at: 3714 4503 3714
Size: 789 7948
PmeI GTTT'AAAC
Cuts at: 6041 6041
Size: 8737
PmlI CAC'GTG
Cuts at: 3982 4441 5638 3982
Size: 459 1197 7081
Fragments arranged by size:
7081 1197 459
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2207 2239 6806 6838 8319 8351 2207
Size: 32 4567 32 1481 32 2593
Fragments arranged by size:
4567 2593 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4126 4528 4897 5079 4126
Size: 402 369 182 7784
Fragments arranged by size:
7784 402 369 182
PshAI GACnn'nnGTC
Cuts at: 1613 3081 3999 5546 1613
Size: 1468 918 1547 4804
Fragments arranged by size:
4804 1547 1468 918
PsiI TTA'TAA
Cuts at: 6307 6307
Size: 8737
PspOMI G'GGCC_C
Cuts at: 4736 5812 4736
Size: 1076 7661
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3803 3835 3803
Size: 32 8705
PstI C_TGCA'G
Cuts at: 830 2748 2901 3660 4008 6089 830
Size: 1918 153 759 348 2081 3478
Fragments arranged by size:
3478 2081 1918 759 348 153
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8737
PvuII CAG'CTG
Cuts at: 2290 2428 2743 3373 4045 5302 5825 2290
Size: 138 315 630 672 1257 523 5202
Fragments arranged by size:
5202 1257 672 630 523 315 138
SacI G_AGCT'C
Cuts at: 2187 4311 2187
Size: 2124 6613
SacII CC_GC'GG
Cuts at: 4155 4467 4598 5279 4155
Size: 312 131 681 7613
Fragments arranged by size:
7613 681 312 131
SalI G'TCGA_C
Cuts at: 1936 3181 3365 3730 6079 1936
Size: 1245 184 365 2349 4594
Fragments arranged by size:
4594 2349 1245 365 184
SanDI GG'GwC_CC
Cuts at: 4897 5079 4897
Size: 182 8555
SapI GCTCTTCn'nnn_
Cuts at: 3772 3772
Size: 8737
SbfI CC_TGCA'GG
Cuts at: 6089 6089
Size: 8737
ScaI AGT'ACT
Cuts at: 6985 8697 6985
Size: 1712 7025
SfcI C'TryA_G
Cuts at: 826 1045 2744 2897 3656 4004 5387 6085 7220
Size: 219 1699 153 759 348 1383 698 1135
Cuts at: 7220 7901 8092 826
Size: 681 191 1471
Fragments arranged by size:
1699 1471 1383 1135 759 698 681 348
219 191 153
SfiI GGCCn_nnn'nGGCC
Cuts at: 4712 4712
Size: 8737
SfoI GGC'GCC
Cuts at: 5614 5614
Size: 8737
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8737
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8737
SmaI CCC'GGG
Cuts at: 1872 2948 4492 4533 4771 5623 6064 1872
Size: 1076 1544 41 238 852 441 4545
Fragments arranged by size:
4545 1544 1076 852 441 238 41
SmlI C'TyrA_G
Cuts at: 820 1017 1943 6841 7742 8004 820
Size: 197 926 4898 901 262 1553
Fragments arranged by size:
4898 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6034 6528 493
Size: 5541 494 2702
Fragments arranged by size:
5541 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8737
SphI G_CATG'C
Cuts at: 1132 6095 1132
Size: 4963 3774
SrfI GCCC'GGGC
Cuts at: 4771 4771
Size: 8737
SspI AAT'ATT
Cuts at: 5 52 6661 5
Size: 47 6609 2081
Fragments arranged by size:
6609 2081 47
StuI AGG'CCT
Cuts at: 1517 4010 1517
Size: 2493 6244
StyI C'CwwG_G
Cuts at: 513 1065 3232 3360 3570 4302 5694 5719 5979
Size: 552 2167 128 210 732 1392 25 260
Cuts at: 5979 6176 513
Size: 197 3074
Fragments arranged by size:
3074 2167 1392 732 552 260 210 197
128 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2312 2615 2833 3002 3720 3844 4260
Size: 1255 732 303 218 169 718 124 416
Cuts at: 4260 4942 5317 5485 6764 6781 6934 7119 7564
Size: 682 375 168 1279 17 153 185 445
Cuts at: 7564 325
Size: 1498
Fragments arranged by size:
1498 1279 1255 732 718 682 445 416
375 303 218 185 169 168 153 124
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2502 2977 3548
Size: 274 80 33 51 1443 525 475 571
Cuts at: 3548 3633 4035 4101 6983 8695 96
Size: 85 402 66 2882 1712 138
Fragments arranged by size:
2882 1712 1443 571 525 475 402 274
138 85 80 66 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8697 8729 8697
Size: 32 8705
Tth111I GACn'n_nGTC
Cuts at: 1316 4813 1316
Size: 3497 5240
XbaI T'CTAG_A
Cuts at: 6073 6073
Size: 8737
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3070 3433 4372 4936 5173 3070
Size: 363 939 564 237 6634
Fragments arranged by size:
6634 939 564 363 237
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8737
XmaI C'CCGG_G
Cuts at: 1870 2946 4490 4531 4769 5621 6062 1870
Size: 1076 1544 41 238 852 441 4545
Fragments arranged by size:
4545 1544 1076 852 441 238 41
XmnI GAAnn'nnTTC
Cuts at: 2651 6866 2651
Size: 4215 4522
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6545 276
Size: 53 83 186 936 5011 2468
Fragments arranged by size:
5011 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BfrBI BstZ17I EcoNI EcoRV
FalI FseI FspAI NruI NsiI PacI PspXI RsrII
SexAI SwaI