Restriction map for
pFN21ASDA1064
(Circular) (Six-base) MAPSORT of: pFN21ASDA1064.seq Check: 4138 from: 1 to: 8638
pFN21ASDA1064 8638 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3476 3476
Size: 8638
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4508 6448 278
Size: 53 83 186 936 2972 1940 2468
Fragments arranged by size:
2972 2468 1940 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5981 1937
Size: 4044 4594
Acc65I G'GTAC_C
Cuts at: 5959 5959
Size: 8638
AclI AA'CG_TT
Cuts at: 1563 6765 7138 1563
Size: 5202 373 3063
Fragments arranged by size:
5202 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2351 3539 4505 4509 5903 6701 8085 8353
Size: 719 1188 966 4 1394 798 1384 268
Cuts at: 8353 1632
Size: 1917
Fragments arranged by size:
1917 1394 1384 1188 966 798 719 268
4
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8441
AflIII A'CryG_T
Cuts at: 1203 3744 7537 8514 1203
Size: 2541 3793 977 1327
Fragments arranged by size:
3793 2541 1327 977
AgeI A'CCGG_T
Cuts at: 7469 7469
Size: 8638
AhdI GACnn_n'nnGTC
Cuts at: 4879 7367 4879
Size: 2488 6150
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8638
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3647 5082 1184
Size: 2463 1435 4740
Fragments arranged by size:
4740 2463 1435
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5573 5605 5573
Size: 32 8606
AlwNI CAG_nnn'CTG
Cuts at: 3730 6381 7953 3730
Size: 2651 1572 4415
Fragments arranged by size:
4415 2651 1572
ApaI G_GGCC'C
Cuts at: 3684 3863 3870 3989 4019 4266 4327 4757 4816
Size: 179 7 119 30 247 61 430 59
Cuts at: 4816 5082 3684
Size: 266 7240
Fragments arranged by size:
7240 430 266 247 179 119 61 59
30 7
ApaLI G'TGCA_C
Cuts at: 5762 6695 7851 5762
Size: 933 1156 6549
Fragments arranged by size:
6549 1156 933
ApoI r'AATT_y
Cuts at: 1493 5947 6173 6325 8434 1493
Size: 4454 226 152 2109 1697
Fragments arranged by size:
4454 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7192 160
Size: 872 6160 1606
Fragments arranged by size:
6160 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8638
AvaI C'yCGr_G
Cuts at: 1870 1943 2036 3018 3677 4856 4964 5101 5235
Size: 73 93 982 659 1179 108 137 134
Cuts at: 5235 5298 5347 5473 5735 5756 5963 1870
Size: 63 49 126 262 21 207 4545
Fragments arranged by size:
4545 1179 982 659 262 207 137 134
126 108 93 73 63 49 21
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4429 4462 4429
Size: 33 8605
BamHI G'GATC_C
Cuts at: 4338 5968 6335 4338
Size: 1630 367 6641
Fragments arranged by size:
6641 1630 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3488 4286 4860 5105 5132
Size: 325 217 627 1701 798 574 245 27
Cuts at: 5132 5326 5525 5648 5741 5959 7414 618
Size: 194 199 123 93 218 1455 1842
Fragments arranged by size:
1842 1701 1455 798 627 574 325 245
218 217 199 194 123 93 27
BanII G_rGCy'C
Cuts at: 1391 3017 3453 3684 3863 3870 3989 4019 4149
Size: 1626 436 231 179 7 119 30 130
Cuts at: 4149 4242 4266 4327 4757 4816 5082 5564 5586
Size: 93 24 61 430 59 266 482 22
Cuts at: 5586 5822 5957 1391
Size: 236 135 4072
Fragments arranged by size:
4072 1626 482 436 430 266 236 231
179 135 130 119 93 61 59 30
24 22 7
BbeI G_GCGC'C
Cuts at: 4290 5109 5136 5330 4290
Size: 819 27 194 7598
Fragments arranged by size:
7598 819 194 27
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3111 4493 928
Size: 971 1212 1382 5073
Fragments arranged by size:
5073 1382 1212 971
BbvCI CC'TCA_GC
Cuts at: 4312 4613 5322 5544 4312
Size: 301 709 222 7406
Fragments arranged by size:
7406 709 301 222
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3199 3233 3276 3310 6829 6863 1903
Size: 34 1262 34 43 34 3519 34 3678
Fragments arranged by size:
3678 3519 1262 43 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2236 6530 7740 2236
Size: 4294 1210 3134
Fragments arranged by size:
4294 3134 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8638
BfrBI ATG'CAT
Cuts at: 3907 3907
Size: 8638
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2135 3060 4023
Size: 107 122 71 631 605 463 925 963
Cuts at: 4023 4322 4752 5573 7249 136
Size: 299 430 821 1676 1525
Fragments arranged by size:
1676 1525 963 925 821 631 605 463
430 299 122 107 71
BglII A'GATC_T
Cuts at: 8633 8633
Size: 8638
BlpI GC'TnA_GC
Cuts at: 4538 5450 6055 4538
Size: 912 605 7121
Fragments arranged by size:
7121 912 605
Bme1580I G_kGCm'C
Cuts at: 1790 3684 3863 3870 3989 4019 4182 4266 4327
Size: 1894 179 7 119 30 163 84 61
Cuts at: 4327 4757 4816 4863 5010 5019 5082 5530 5651
Size: 430 59 47 147 9 63 448 121
Cuts at: 5651 5746 5766 5781 6699 7855 1790
Size: 95 20 15 918 1156 2573
Fragments arranged by size:
2573 1894 1156 918 448 430 179 163
147 121 119 95 84 63 61 59
47 30 20 15 9 7
BmgBI CAC'GTC
Cuts at: 1318 4918 1318
Size: 3600 5038
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2457 3393 5220
Size: 416 53 83 399 24 1040 936 1827
Cuts at: 5220 5423 6369 7327 442
Size: 203 946 958 1753
Fragments arranged by size:
1827 1753 1040 958 946 936 416 399
203 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8638
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5347 5347
Size: 8638
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4071 4256 4553 7298 1239
Size: 2832 185 297 2745 2579
Fragments arranged by size:
2832 2745 2579 297 185
Bpu10I CC'TnA_GC
Cuts at: 4224 4312 4613 4925 5322 5544 5577 5821 4224
Size: 88 301 312 397 222 33 244 7041
Fragments arranged by size:
7041 397 312 301 244 222 88 33
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4426 4513 5156 6763 7628 7926 4426
Size: 87 643 1607 865 298 5138
Fragments arranged by size:
5138 1607 865 643 298 87
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2228 2841 3237 7301 882
Size: 617 729 613 396 4064 2219
Fragments arranged by size:
4064 2219 729 617 613 396
BsaAI yAC'GTr
Cuts at: 493 1204 5935 6429 493
Size: 711 4731 494 2702
Fragments arranged by size:
4731 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 8632 8632
Size: 8638
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2127 4287 4505 5106
Size: 53 83 186 936 594 2160 218 601
Cuts at: 5106 5133 5327 6445 6827 7462 275
Size: 27 194 1118 382 635 1451
Fragments arranged by size:
2160 1451 1118 936 635 601 594 382
218 194 186 83 53 27
BsaWI w'CCGG_w
Cuts at: 2249 3150 4427 7071 7469 7743 7890 8301 2249
Size: 901 1277 2644 398 274 147 411 2586
Fragments arranged by size:
2644 2586 1277 901 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2924 2951 2954 2981 5573
Size: 30 165 30 1521 27 3 27 2592
Cuts at: 5573 5603 1178
Size: 30 4213
Fragments arranged by size:
4213 2592 1521 165 30 30 30 27
27 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2100 2141 2246 2324 2398 2660 2663 2859
Size: 914 41 105 78 74 262 3 196
Cuts at: 2859 2921 3084 3573 4373 4376 4379 4390 4808
Size: 62 163 489 800 3 3 11 418
Cuts at: 4808 4811 5185 5284 1186
Size: 3 374 99 4540
Fragments arranged by size:
4540 914 800 489 418 374 262 196
163 105 99 78 74 62 41 11
3 3 3 3
BseYI C'CCAG_C
Cuts at: 3814 4552 4757 4934 5529 5751 5837 7841 8337
Size: 738 205 177 595 222 86 2004 496
Cuts at: 8337 3814
Size: 4115
Fragments arranged by size:
4115 2004 738 595 496 222 205 177
86
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3217 5042 1163
Size: 573 1481 1825 4759
Fragments arranged by size:
4759 1825 1481 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2734 4277 5202 5406 6088 6849
Size: 275 186 733 1543 925 204 682 761
Cuts at: 6849 7877 1540
Size: 1028 2301
Fragments arranged by size:
2301 1543 1028 925 761 733 682 275
204 186
BsiHKAI G_wGCw'C
Cuts at: 3453 4215 4230 4956 5564 5766 6699 6784 7855
Size: 762 15 726 608 202 933 85 1071
Cuts at: 7855 3453
Size: 4236
Fragments arranged by size:
4236 1071 933 762 726 608 202 85
15
BsmI GAATG_Cn'
Cuts at: 6149 6242 6149
Size: 93 8545
BsmBI CGTCTCn'nnnn_
Cuts at: 2913 3434 2913
Size: 521 8117
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3017 3453 3684 3863 3870 3989 4019
Size: 399 1227 436 231 179 7 119 30
Cuts at: 4019 4149 4182 4215 4230 4242 4266 4327 4757
Size: 130 33 33 15 12 24 61 430
Cuts at: 4757 4816 4863 4956 5010 5019 5082 5530 5564
Size: 59 47 93 54 9 63 448 34
Cuts at: 5564 5586 5651 5746 5766 5781 5822 5957 6699
Size: 22 65 95 20 15 41 135 742
Cuts at: 6699 6784 7855 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1227 1071 742 448 436 430 399
231 179 135 130 119 95 93 85
65 63 61 59 54 47 41 34
33 33 30 24 22 20 15 15
12 9 7
BspEI T'CCGG_A
Cuts at: 2249 8301 2249
Size: 6052 2586
BspHI T'CATG_A
Cuts at: 6527 8257 6527
Size: 1730 6908
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3476 5778 5993 844
Size: 2632 2302 215 3489
Fragments arranged by size:
3489 2632 2302 215
BsrBI CCG'CTC
Cuts at: 1394 1823 2170 2353 2967 4810 5378 5454 6525
Size: 429 347 183 614 1843 568 76 1071
Cuts at: 6525 1394
Size: 3507
Fragments arranged by size:
3507 1843 1071 614 568 429 347 183
76
BsrDI GCAATG_nn'
Cuts at: 65 1248 5557 7133 7307 65
Size: 1183 4309 1576 174 1396
Fragments arranged by size:
4309 1576 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2703 3700 4272 4289 4593 5189 5405
Size: 110 893 997 572 17 304 596 216
Cuts at: 5405 5509 5633 5679 7282 7469 1700
Size: 104 124 46 1603 187 2869
Fragments arranged by size:
2869 1603 997 893 596 572 304 216
187 124 110 104 46 17
BsrGI T'GTAC_A
Cuts at: 96 3747 96
Size: 3651 4987
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8638
BssSI C'ACGA_G
Cuts at: 6698 7710 6698
Size: 1012 7626
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3730 4867 5035 5770 794
Size: 2936 1137 168 735 3662
Fragments arranged by size:
3662 2936 1137 735 168
BstBI TT'CG_AA
Cuts at: 2007 7442 2007
Size: 5435 3203
BstEII G'GTnAC_C
Cuts at: 1184 2474 1184
Size: 1290 7348
BstXI CCAn_nnnn'nTGG
Cuts at: 3383 4750 3383
Size: 1367 7271
BstYI r'GATC_y
Cuts at: 1972 3759 4338 5968 6335 6720 6737 8189 8275
Size: 1787 579 1630 367 385 17 1452 86
Cuts at: 8275 8287 8633 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1787 1630 1452 579 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4334 4334
Size: 8638
BtgI C'CryG_G
Cuts at: 513 1065 2022 2728 2779 2797 3004 3792 4011
Size: 552 957 706 51 18 207 788 219
Cuts at: 4011 4877 5019 5070 5112 5655 5910 513
Size: 866 142 51 42 543 255 3241
Fragments arranged by size:
3241 957 866 788 706 552 543 255
219 207 142 51 51 42 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2064 2436 2520 487
Size: 683 56 838 372 84 6605
Fragments arranged by size:
6605 838 683 372 84 56
BtsI GCAGTG_nn'
Cuts at: 4474 4552 5010 5449 6154 6948 6968 4474
Size: 78 458 439 705 794 20 6144
Fragments arranged by size:
6144 794 705 458 439 78 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8638
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8603
DraI TTT'AAA
Cuts at: 5896 5942 5896
Size: 46 8592
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7645 809
Size: 507 6329 1802
Fragments arranged by size:
6329 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2136 2731 3468
Size: 54 1425 50 275 18 306 595 737
Cuts at: 3468 4274 5199 5403 6085 6974 8603 8
Size: 806 925 204 682 889 1629 43
Fragments arranged by size:
1629 1425 925 889 806 737 682 595
306 275 204 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2731 4274 5199 5403 6085 1537
Size: 275 919 1543 925 204 682 4090
Fragments arranged by size:
4090 1543 925 919 682 275 204
EarI CTCTTCn'nnn_
Cuts at: 1353 2375 2859 2877 3432 4182 4223 5792 6568
Size: 1022 484 18 555 750 41 1569 776
Cuts at: 6568 1353
Size: 3423
Fragments arranged by size:
3423 1569 1022 776 750 555 484 41
18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3102 5439 7225 7599 7745 387
Size: 1064 138 1513 2337 1786 374 146 1280
Fragments arranged by size:
2337 1786 1513 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3451 5562 3451
Size: 2111 6527
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2351 3539 4071 4256 4505 4509 4553
Size: 393 719 1188 532 185 249 4 44
Cuts at: 4553 5903 6701 7298 8085 8353 1239
Size: 1350 798 597 787 268 1524
Fragments arranged by size:
1524 1350 1188 798 787 719 597 532
393 268 249 185 44 4
EcoNI CCTnn'n_nnAGG
Cuts at: 4571 4571
Size: 8638
EcoO109I rG'GnC_Cy
Cuts at: 2040 2255 3681 3860 3878 3986 4016 4262 4263
Size: 215 1426 179 18 108 30 246 1
Cuts at: 4263 4323 4324 4558 4621 4679 4754 4813 4873
Size: 60 1 234 63 58 75 59 60
Cuts at: 4873 4969 5078 5079 5248 2040
Size: 96 109 1 169 5430
Fragments arranged by size:
5430 1426 246 234 215 179 169 109
108 96 75 63 60 60 59 58
30 18 1 1 1
EcoRI G'AATT_C
Cuts at: 5947 6325 5947
Size: 378 8260
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4751 4954 4751
Size: 203 8435
FseI GG_CCGG'CC
Cuts at: 4276 4276
Size: 8638
FspI TGC'GCA
Cuts at: 7144 7144
Size: 8638
HaeII r_GCGC'y
Cuts at: 1678 3232 4290 5101 5109 5136 5330 7785 1678
Size: 1554 1058 811 8 27 194 2455 2531
Fragments arranged by size:
2531 2455 1554 1058 811 194 27 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2300 2332 2414 2446 2924
Size: 32 135 32 728 32 82 32 478
Cuts at: 2924 2956 3701 3733 4235 4267 4865 4897 5861
Size: 32 745 32 502 32 598 32 964
Cuts at: 5861 5893 7279 7311 7353 7385 1373
Size: 32 1386 32 42 32 2626
Fragments arranged by size:
2626 1386 964 745 728 598 502 478
135 82 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5982 6228 669
Size: 988 281 4044 246 3079
Fragments arranged by size:
4044 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8638
HpaI GTT'AAC
Cuts at: 6228 6228
Size: 8638
KasI G'GCGC_C
Cuts at: 4286 5105 5132 5326 4286
Size: 819 27 194 7598
Fragments arranged by size:
7598 819 194 27
KpnI G_GTAC'C
Cuts at: 5963 5963
Size: 8638
MfeI C'AATT_G
Cuts at: 6237 6237
Size: 8638
MluI A'CGCG_T
Cuts at: 8514 8514
Size: 8638
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2178 3165 3632 7752 7936 1305
Size: 873 987 467 4120 184 2007
Fragments arranged by size:
4120 2007 987 873 467 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6816
Fragments arranged by size:
6816 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2295 3701 3980 4139 6596 6955 7114
Size: 648 1129 1406 279 159 2457 359 159
Cuts at: 7114 518
Size: 2042
Fragments arranged by size:
2457 2042 1406 1129 648 359 279 159
159
MspA1I CmG'CkG
Cuts at: 1606 2730 2799 3730 3802 4888 5021 5711 6054
Size: 1124 69 931 72 1086 133 690 343
Cuts at: 6054 6731 7879 8124 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 1124 1086 931 690 677 343
245 133 72 69
NaeI GCC'GGC
Cuts at: 4274 4595 5511 4274
Size: 321 916 7401
Fragments arranged by size:
7401 916 321
NarI GG'CG_CC
Cuts at: 4287 5106 5133 5327 4287
Size: 819 27 194 7598
Fragments arranged by size:
7598 819 194 27
NcoI C'CATG_G
Cuts at: 513 1065 2022 4011 513
Size: 552 957 1989 5140
Fragments arranged by size:
5140 1989 957 552
NdeI CA'TA_TG
Cuts at: 387 2506 387
Size: 2119 6519
NgoMIV G'CCGG_C
Cuts at: 4272 4593 5509 4272
Size: 321 916 7401
Fragments arranged by size:
7401 916 321
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8638
NotI GC'GGCC_GC
Cuts at: 6085 6085
Size: 8638
NruI TCG'CGA
Cuts at: 2791 2791
Size: 8638
NsiI A_TGCA'T
Cuts at: 3909 3909
Size: 8638
NspI r_CATG'y
Cuts at: 1132 1229 2543 2599 3415 3748 3919 3931 3943
Size: 97 1314 56 816 333 171 12 12
Cuts at: 3943 3955 3967 3979 5996 7541 1132
Size: 12 12 12 2017 1545 2229
Fragments arranged by size:
2229 2017 1545 1314 816 333 171 97
56 12 12 12 12 12
PasI CC'CwG_GG
Cuts at: 1348 3848 3863 3884 1348
Size: 2500 15 21 6102
Fragments arranged by size:
6102 2500 21 15
PciI A'CATG_T
Cuts at: 3744 7537 3744
Size: 3793 4845
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4067 5837 1108
Size: 2959 1770 3909
Fragments arranged by size:
3909 2959 1770
PmeI GTTT'AAAC
Cuts at: 5942 5942
Size: 8638
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5573 5605 6707 6739 8220 8252 5573
Size: 32 1102 32 1481 32 5959
Fragments arranged by size:
5959 1481 1102 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2040 3878 4558 4621 4679 4873 4969 2040
Size: 1838 680 63 58 194 96 5709
Fragments arranged by size:
5709 1838 680 194 96 63 58
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8638
PsiI TTA'TAA
Cuts at: 6208 6208
Size: 8638
PspOMI G'GGCC_C
Cuts at: 3680 3859 3866 3985 4015 4262 4323 4753 4812
Size: 179 7 119 30 247 61 430 59
Cuts at: 4812 5078 3680
Size: 266 7240
Fragments arranged by size:
7240 430 266 247 179 119 61 59
30 7
PstI C_TGCA'G
Cuts at: 830 3735 4893 5271 5704 5990 830
Size: 2905 1158 378 433 286 3478
Fragments arranged by size:
3478 2905 1158 433 378 286
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8638
PvuII CAG'CTG
Cuts at: 3730 3802 4888 3730
Size: 72 1086 7480
Fragments arranged by size:
7480 1086 72
SacI G_AGCT'C
Cuts at: 3453 5564 3453
Size: 2111 6527
SacII CC_GC'GG
Cuts at: 2731 2800 5022 2731
Size: 69 2222 6347
Fragments arranged by size:
6347 2222 69
SalI G'TCGA_C
Cuts at: 1936 5980 1936
Size: 4044 4594
SanDI GG'GwC_CC
Cuts at: 2040 3878 4558 4621 4679 4873 2040
Size: 1838 680 63 58 194 5805
Fragments arranged by size:
5805 1838 680 194 63 58
SapI GCTCTTCn'nnn_
Cuts at: 4182 4182
Size: 8638
SbfI CC_TGCA'GG
Cuts at: 5990 5990
Size: 8638
ScaI AGT'ACT
Cuts at: 2939 3215 6886 8598 2939
Size: 276 3671 1712 2979
Fragments arranged by size:
3671 2979 1712 276
SfcI C'TryA_G
Cuts at: 826 1045 2761 3731 4889 5267 5700 5986 7121
Size: 219 1716 970 1158 378 433 286 1135
Cuts at: 7121 7802 7993 826
Size: 681 191 1471
Fragments arranged by size:
1716 1471 1158 1135 970 681 433 378
286 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 4023 4023
Size: 8638
SfoI GGC'GCC
Cuts at: 4288 5107 5134 5328 4288
Size: 819 27 194 7598
Fragments arranged by size:
7598 819 194 27
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8638
SgrAI Cr'CCGG_yG
Cuts at: 1700 4289 1700
Size: 2589 6049
SmaI CCC'GGG
Cuts at: 1872 2038 3679 4858 5103 5237 5300 5758 5965
Size: 166 1641 1179 245 134 63 458 207
Cuts at: 5965 1872
Size: 4545
Fragments arranged by size:
4545 1641 1179 458 245 207 166 134
63
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4441 4492 5171 6742 7643 7905
Size: 197 926 2498 51 679 1571 901 262
Cuts at: 7905 820
Size: 1553
Fragments arranged by size:
2498 1571 1553 926 901 679 262 197
51
SnaBI TAC'GTA
Cuts at: 493 5935 6429 493
Size: 5442 494 2702
Fragments arranged by size:
5442 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8638
SphI G_CATG'C
Cuts at: 1132 2543 5996 1132
Size: 1411 3453 3774
Fragments arranged by size:
3774 3453 1411
SrfI GCCC'GGGC
Cuts at: 3679 5103 3679
Size: 1424 7214
SspI AAT'ATT
Cuts at: 5 52 6562 5
Size: 47 6510 2081
Fragments arranged by size:
6510 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8638
StyI C'CwwG_G
Cuts at: 513 1065 2022 2712 2943 2970 4011 4767 5535
Size: 552 957 690 231 27 1041 756 768
Cuts at: 5535 5571 5691 6077 513
Size: 36 120 386 3074
Fragments arranged by size:
3074 1041 957 768 756 690 552 386
231 120 36 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3449 3806 4024 5824 6665 6682 6835
Size: 1255 1869 357 218 1800 841 17 153
Cuts at: 6835 7020 7465 325
Size: 185 445 1498
Fragments arranged by size:
1869 1800 1498 1255 841 445 357 218
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2484 2493 2937
Size: 274 80 33 51 1443 507 9 444
Cuts at: 2937 3213 3747 6884 8596 96
Size: 276 534 3137 1712 138
Fragments arranged by size:
3137 1712 1443 534 507 444 276 274
138 80 51 33 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5620 5652 8598 8630 5620
Size: 32 2946 32 5628
Fragments arranged by size:
5628 2946 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8638
XbaI T'CTAG_A
Cuts at: 5974 5974
Size: 8638
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3799 5041 3799
Size: 1242 7396
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8638
XmaI C'CCGG_G
Cuts at: 1870 2036 3677 4856 5101 5235 5298 5756 5963
Size: 166 1641 1179 245 134 63 458 207
Cuts at: 5963 1870
Size: 4545
Fragments arranged by size:
4545 1641 1179 458 245 207 166 134
63
XmnI GAAnn'nnTTC
Cuts at: 6767 6767
Size: 8638
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4506 6446 276
Size: 53 83 186 936 2972 1940 2468
Fragments arranged by size:
2972 2468 1940 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BsiWI BstZ17I DraIII EcoRV FspAI
PacI PfoI PmlI PspXI PsrI RsrII SexAI SwaI