Restriction map for
pFN21ASDA1059
(Circular) (Six-base) MAPSORT of: pFN21ASDA1059.seq Check: 825 from: 1 to: 8395
pFN21ASDA1059 8395 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2317 3140 3684 4146 2317
Size: 823 544 462 6566
Fragments arranged by size:
6566 823 544 462
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6205 278
Size: 53 83 186 936 4669 2468
Fragments arranged by size:
4669 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2099 2720 5738 1937
Size: 162 621 3018 4594
Fragments arranged by size:
4594 3018 621 162
Acc65I G'GTAC_C
Cuts at: 5716 5716
Size: 8395
AclI AA'CG_TT
Cuts at: 1563 6522 6895 1563
Size: 4959 373 3063
Fragments arranged by size:
4959 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2577 4030 4081 4621 5169 5333 6458 7842
Size: 945 1453 51 540 548 164 1125 1384
Cuts at: 7842 8110 1632
Size: 268 1917
Fragments arranged by size:
1917 1453 1384 1125 945 548 540 268
164 51
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8198
AflIII A'CryG_T
Cuts at: 1203 3264 3393 3419 4665 7294 8271 1203
Size: 2061 129 26 1246 2629 977 1327
Fragments arranged by size:
2629 2061 1327 1246 977 129 26
AgeI A'CCGG_T
Cuts at: 7226 7226
Size: 8395
AhdI GACnn_n'nnGTC
Cuts at: 2145 7124 2145
Size: 4979 3416
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8395
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8395
AlwNI CAG_nnn'CTG
Cuts at: 2387 2920 6138 7710 2387
Size: 533 3218 1572 3072
Fragments arranged by size:
3218 3072 1572 533
ApaLI G'TGCA_C
Cuts at: 2624 3086 3638 6452 7608 2624
Size: 462 552 2814 1156 3411
Fragments arranged by size:
3411 2814 1156 552 462
ApoI r'AATT_y
Cuts at: 1493 5293 5704 5930 6082 8191 1493
Size: 3800 411 226 152 2109 1697
Fragments arranged by size:
3800 2109 1697 411 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6949 160
Size: 872 5917 1606
Fragments arranged by size:
5917 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8395
AvaI C'yCGr_G
Cuts at: 1870 1943 2514 4485 5720 1870
Size: 73 571 1971 1235 4545
Fragments arranged by size:
4545 1971 1235 571 73
BamHI G'GATC_C
Cuts at: 3138 5725 6092 3138
Size: 2587 367 5441
Fragments arranged by size:
5441 2587 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2065 2117 2268 2530 2732
Size: 325 217 627 278 52 151 262 202
Cuts at: 2732 3594 3875 4134 5716 7171 618
Size: 862 281 259 1582 1455 1842
Fragments arranged by size:
1842 1582 1455 862 627 325 281 278
262 259 217 202 151 52
BanII G_rGCy'C
Cuts at: 1391 2088 3774 3997 4207 5119 5714 1391
Size: 697 1686 223 210 912 595 4072
Fragments arranged by size:
4072 1686 912 697 595 223 210
BbeI G_GCGC'C
Cuts at: 2069 2121 2272 2534 3598 2069
Size: 52 151 262 1064 6866
Fragments arranged by size:
6866 1064 262 151 52
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2565 2777 3007 3566 928
Size: 971 666 212 230 559 5757
Fragments arranged by size:
5757 971 666 559 230 212
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2566 2600 6586 6620 1903
Size: 34 629 34 3986 34 3678
Fragments arranged by size:
3986 3678 629 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6287 7497 6287
Size: 1210 7185
BclI T'GATC_A
Cuts at: 1548 2859 1548
Size: 1311 7084
BfrBI ATG'CAT
Cuts at: 3019 3019
Size: 8395
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2052 3884 3913
Size: 107 122 71 631 605 380 1832 29
Cuts at: 3913 7006 136
Size: 3093 1525
Fragments arranged by size:
3093 1832 1525 631 605 380 122 107
71 29
BglII A'GATC_T
Cuts at: 3831 8390 3831
Size: 4559 3836
BlpI GC'TnA_GC
Cuts at: 5395 5812 5395
Size: 417 7978
Bme1580I G_kGCm'C
Cuts at: 1790 2628 3090 3642 3880 4216 4472 4539 5388
Size: 838 462 552 238 336 256 67 849
Cuts at: 5388 6456 7612 1790
Size: 1068 1156 2573
Fragments arranged by size:
2573 1156 1068 849 838 552 462 336
256 238 67
BmgBI CAC'GTC
Cuts at: 1318 2103 1318
Size: 785 7610
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2956 3629 3877
Size: 416 53 83 399 24 1539 673 248
Cuts at: 3877 4404 4833 4856 6126 7084 442
Size: 527 429 23 1270 958 1753
Fragments arranged by size:
1753 1539 1270 958 673 527 429 416
399 248 83 53 24 23
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8395
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3961 3961
Size: 8395
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5359 7055 1239
Size: 4120 1696 2579
Fragments arranged by size:
4120 2579 1696
Bpu10I CC'TnA_GC
Cuts at: 2505 4436 2505
Size: 1931 6464
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5478 6520 7385 7683 5478
Size: 1042 865 298 6190
Fragments arranged by size:
6190 1042 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4010 4218 4249 5180 7058 882
Size: 617 2511 208 31 931 1878 2219
Fragments arranged by size:
2511 2219 1878 931 617 208 31
BsaAI yAC'GTr
Cuts at: 493 1204 3265 3396 4666 5692 6186 493
Size: 711 2061 131 1270 1026 494 2702
Fragments arranged by size:
2702 2061 1270 1026 711 494 131
BsaBI GATnn'nnATC
Cuts at: 5483 8389 5483
Size: 2906 5489
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2066 2118 2155 2240
Size: 53 83 186 936 533 52 37 85
Cuts at: 2240 2269 2454 2531 3595 6202 6584 7219 275
Size: 29 185 77 1064 2607 382 635 1451
Fragments arranged by size:
2607 1451 1064 936 635 533 382 186
185 85 83 77 53 52 37 29
BsaWI w'CCGG_w
Cuts at: 2076 3610 6828 7226 7500 7647 8058 2076
Size: 1534 3218 398 274 147 411 2413
Fragments arranged by size:
3218 2413 1534 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2225 2255 4301 4331 5344
Size: 30 165 30 822 30 2046 30 1013
Cuts at: 5344 5374 1178
Size: 30 4199
Fragments arranged by size:
4199 2046 1013 822 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2246 2422 2425 2534 3793 4580 5242 5603
Size: 1060 176 3 109 1259 787 662 361
Cuts at: 5603 1186
Size: 3978
Fragments arranged by size:
3978 1259 1060 787 662 361 176 109
3
BseYI C'CCAG_C
Cuts at: 2263 2295 2380 2540 2573 4324 4970 5028 5381
Size: 32 85 160 33 1751 646 58 353
Cuts at: 5381 7598 8094 2263
Size: 2217 496 2564
Fragments arranged by size:
2564 2217 1751 646 496 353 160 85
58 33 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3656 4408 4845 5662 1163
Size: 573 1920 752 437 817 3896
Fragments arranged by size:
3896 1920 817 752 573 437
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2475 4509 5845 6606 7634 1540
Size: 275 186 474 2034 1336 761 1028 2301
Fragments arranged by size:
2301 2034 1336 1028 761 474 275 186
BsiHKAI G_wGCw'C
Cuts at: 2628 3090 3642 3774 4207 5119 6456 6541 7612
Size: 462 552 132 433 912 1337 85 1071
Cuts at: 7612 2628
Size: 3411
Fragments arranged by size:
3411 1337 1071 912 552 462 433 132
85
BsmI GAATG_Cn'
Cuts at: 4981 5906 5999 4981
Size: 925 93 7377
Fragments arranged by size:
7377 925 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2088 2628 3090 3642 3774 3880 3997
Size: 399 298 540 462 552 132 106 117
Cuts at: 3997 4207 4216 4472 4539 5119 5388 5714 6456
Size: 210 9 256 67 580 269 326 742
Cuts at: 6456 6541 7612 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 580 552 540 462 399
326 298 269 256 210 132 117 106
85 67 9
BspEI T'CCGG_A
Cuts at: 2076 3610 8058 2076
Size: 1534 4448 2413
Fragments arranged by size:
4448 2413 1534
BspHI T'CATG_A
Cuts at: 4026 5011 6284 8014 4026
Size: 985 1273 1730 4407
Fragments arranged by size:
4407 1730 1273 985
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2317 3140 3500 3684 4146 5750 844
Size: 1473 823 360 184 462 1604 3489
Fragments arranged by size:
3489 1604 1473 823 462 360 184
BsrBI CCG'CTC
Cuts at: 1394 1823 2362 3183 4056 6282 1394
Size: 429 539 821 873 2226 3507
Fragments arranged by size:
3507 2226 873 821 539 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4105 5107 5157 5245 5631 6890 7064
Size: 1183 2857 1002 50 88 386 1259 174
Cuts at: 7064 65
Size: 1396
Fragments arranged by size:
2857 1396 1259 1183 1002 386 174 88
50
BsrFI r'CCGG_y
Cuts at: 1700 1810 2114 2182 2350 4101 7039 7226 1700
Size: 110 304 68 168 1751 2938 187 2869
Fragments arranged by size:
2938 2869 1751 304 187 168 110 68
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8395
BssHII G'CGCG_C
Cuts at: 1925 2033 1925
Size: 108 8287
BssSI C'ACGA_G
Cuts at: 3881 6455 7467 3881
Size: 2574 1012 4809
Fragments arranged by size:
4809 2574 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3016 3646 794
Size: 2222 630 5543
Fragments arranged by size:
5543 2222 630
BstBI TT'CG_AA
Cuts at: 2007 7199 2007
Size: 5192 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8395
BstXI CCAn_nnnn'nTGG
Cuts at: 4332 4846 4332
Size: 514 7881
BstYI r'GATC_y
Cuts at: 1972 3138 3831 4683 5151 5667 5725 6092 6477
Size: 1166 693 852 468 516 58 367 385
Cuts at: 6477 6494 7946 8032 8044 8390 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1166 852 693 516 468 385
367 346 86 58 17 12
Bsu36I CC'TnA_GG
Cuts at: 3703 5586 3703
Size: 1883 6512
BtgI C'CryG_G
Cuts at: 513 1065 2022 2204 2534 2619 2988 3105 3444
Size: 552 957 182 330 85 369 117 339
Cuts at: 3444 513
Size: 5464
Fragments arranged by size:
5464 957 552 369 339 330 182 117
85
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7656
Fragments arranged by size:
7656 683 56
BtsI GCAGTG_nn'
Cuts at: 2217 2463 3640 5911 6705 6725 2217
Size: 246 1177 2271 794 20 3887
Fragments arranged by size:
3887 2271 1177 794 246 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8395
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2169 2204 529
Size: 35 1605 35 6720
Fragments arranged by size:
6720 1605 35 35
DraI TTT'AAA
Cuts at: 5461 5699 5461
Size: 238 8157
DraIII CAC_nnn'GTG
Cuts at: 5570 5570
Size: 8395
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4733 5618 7402 809
Size: 507 3417 885 1784 1802
Fragments arranged by size:
3417 1802 1784 885 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2112 2177 2214
Size: 54 1425 50 275 18 282 65 37
Cuts at: 2214 2225 2472 3057 3441 3885 4039 5842 6731
Size: 11 247 585 384 444 154 1803 889
Cuts at: 6731 8360 8
Size: 1629 43
Fragments arranged by size:
1803 1629 1425 889 585 444 384 282
275 247 154 65 54 50 43 37
18 11
EagI C'GGCC_G
Cuts at: 1537 1812 2472 5842 1537
Size: 275 660 3370 4090
Fragments arranged by size:
4090 3370 660 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3244 3271 3837 4745 6325 1353
Size: 1891 27 566 908 1580 3423
Fragments arranged by size:
3423 1891 1580 908 566 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3092 4420 6982 7356 7502 387
Size: 1064 138 1503 1328 2562 374 146 1280
Fragments arranged by size:
2562 1503 1328 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3772 4205 5117 3772
Size: 433 912 7050
Fragments arranged by size:
7050 912 433
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2577 4030 4081 4621 5169 5333 5359
Size: 393 945 1453 51 540 548 164 26
Cuts at: 5359 6458 7055 7842 8110 1239
Size: 1099 597 787 268 1524
Fragments arranged by size:
1524 1453 1099 945 787 597 548 540
393 268 164 51 26
EcoNI CCTnn'n_nnAGG
Cuts at: 2504 4562 4985 2504
Size: 2058 423 5914
Fragments arranged by size:
5914 2058 423
EcoO109I rG'GnC_Cy
Cuts at: 2107 4489 4851 5248 2107
Size: 2382 362 397 5254
Fragments arranged by size:
5254 2382 397 362
EcoRI G'AATT_C
Cuts at: 5704 6082 5704
Size: 378 8017
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3189 3189
Size: 8395
FspI TGC'GCA
Cuts at: 6901 6901
Size: 8395
HaeII r_GCGC'y
Cuts at: 1678 2047 2069 2121 2272 2534 3598 7542 1678
Size: 369 22 52 151 262 1064 3944 2531
Fragments arranged by size:
3944 2531 1064 369 262 151 52 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2131 2163 2225 2257 2398
Size: 32 135 32 559 32 62 32 141
Cuts at: 2398 2430 4073 4105 4227 4259 5344 5376 7036
Size: 32 1643 32 122 32 1085 32 1660
Cuts at: 7036 7068 7110 7142 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1660 1643 1085 559 141 135 122
62 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2100 2721 3988 4816 4844 5739
Size: 988 281 162 621 1267 828 28 895
Cuts at: 5739 5985 669
Size: 246 3079
Fragments arranged by size:
3079 1267 988 895 828 621 281 246
162 28
HindIII A'AGCT_T
Cuts at: 748 2393 748
Size: 1645 6750
HpaI GTT'AAC
Cuts at: 5985 5985
Size: 8395
KasI G'GCGC_C
Cuts at: 2065 2117 2268 2530 3594 2065
Size: 52 151 262 1064 6866
Fragments arranged by size:
6866 1064 262 151 52
KpnI G_GTAC'C
Cuts at: 5720 5720
Size: 8395
MfeI C'AATT_G
Cuts at: 4067 4267 5994 4067
Size: 200 1727 6468
Fragments arranged by size:
6468 1727 200
MluI A'CGCG_T
Cuts at: 3419 8271 3419
Size: 4852 3543
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2825 4440 5524 7509 7693 1305
Size: 1520 1615 1084 1985 184 2007
Fragments arranged by size:
2007 1985 1615 1520 1084 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2216 3443 3887 4041 10
Size: 54 1425 343 384 1227 444 154 4364
Fragments arranged by size:
4364 1425 1227 444 384 343 154 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3095 4559 5162 5405 6353 6712 6871
Size: 648 1929 1464 603 243 948 359 159
Cuts at: 6871 518
Size: 2042
Fragments arranged by size:
2042 1929 1464 948 648 603 359 243
159
MspA1I CmG'CkG
Cuts at: 1606 2206 2536 4733 5350 5811 6488 7636 7881
Size: 600 330 2197 617 461 677 1148 245
Cuts at: 7881 1606
Size: 2120
Fragments arranged by size:
2197 2120 1148 677 617 600 461 330
245
NaeI GCC'GGC
Cuts at: 2116 4103 2116
Size: 1987 6408
NarI GG'CG_CC
Cuts at: 2066 2118 2269 2531 3595 2066
Size: 52 151 262 1064 6866
Fragments arranged by size:
6866 1064 262 151 52
NcoI C'CATG_G
Cuts at: 513 1065 2022 2619 2988 3444 513
Size: 552 957 597 369 456 5464
Fragments arranged by size:
5464 957 597 552 456 369
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8395
NgoMIV G'CCGG_C
Cuts at: 2114 4101 2114
Size: 1987 6408
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8395
NotI GC'GGCC_GC
Cuts at: 5842 5842
Size: 8395
NsiI A_TGCA'T
Cuts at: 3021 3021
Size: 8395
NspI r_CATG'y
Cuts at: 1132 1229 3331 4751 4772 5753 7298 1132
Size: 97 2102 1420 21 981 1545 2229
Fragments arranged by size:
2229 2102 1545 1420 981 97 21
PasI CC'CwG_GG
Cuts at: 1348 4216 1348
Size: 2868 5527
PciI A'CATG_T
Cuts at: 7294 7294
Size: 8395
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2223 2620 5296 1108
Size: 1115 397 2676 4207
Fragments arranged by size:
4207 2676 1115 397
PfoI T'CCnGG_A
Cuts at: 2149 3141 3210 4986 2149
Size: 992 69 1776 5558
Fragments arranged by size:
5558 1776 992 69
PmeI GTTT'AAAC
Cuts at: 5699 5699
Size: 8395
PmlI CAC'GTG
Cuts at: 4666 4666
Size: 8395
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6464 6496 7977 8009 6464
Size: 32 1481 32 6850
Fragments arranged by size:
6850 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2107 4851 5248 2107
Size: 2744 397 5254
Fragments arranged by size:
5254 2744 397
PshAI GACnn'nnGTC
Cuts at: 1613 2105 1613
Size: 492 7903
PsiI TTA'TAA
Cuts at: 5965 5965
Size: 8395
PstI C_TGCA'G
Cuts at: 830 3137 4367 5747 830
Size: 2307 1230 1380 3478
Fragments arranged by size:
3478 2307 1380 1230
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8395
PvuII CAG'CTG
Cuts at: 4733 5350 4733
Size: 617 7778
SacI G_AGCT'C
Cuts at: 3774 4207 5119 3774
Size: 433 912 7050
Fragments arranged by size:
7050 912 433
SacII CC_GC'GG
Cuts at: 2207 2537 2207
Size: 330 8065
SalI G'TCGA_C
Cuts at: 1936 2098 2719 5737 1936
Size: 162 621 3018 4594
Fragments arranged by size:
4594 3018 621 162
SanDI GG'GwC_CC
Cuts at: 4851 4851
Size: 8395
SapI GCTCTTCn'nnn_
Cuts at: 3244 3244
Size: 8395
SbfI CC_TGCA'GG
Cuts at: 3137 5747 3137
Size: 2610 5785
ScaI AGT'ACT
Cuts at: 3311 6643 8355 3311
Size: 3332 1712 3351
Fragments arranged by size:
3351 3332 1712
SexAI A'CCwGG_T
Cuts at: 3081 3552 3081
Size: 471 7924
SfcI C'TryA_G
Cuts at: 826 1045 2959 2980 3133 3750 4363 5743 6878
Size: 219 1914 21 153 617 613 1380 1135
Cuts at: 6878 7559 7750 826
Size: 681 191 1471
Fragments arranged by size:
1914 1471 1380 1135 681 617 613 219
191 153 21
SfoI GGC'GCC
Cuts at: 2067 2119 2270 2532 3596 2067
Size: 52 151 262 1064 6866
Fragments arranged by size:
6866 1064 262 151 52
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8395
SgrAI Cr'CCGG_yG
Cuts at: 1700 2182 1700
Size: 482 7913
SmaI CCC'GGG
Cuts at: 1872 2516 5722 1872
Size: 644 3206 4545
Fragments arranged by size:
4545 3206 644
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5493 6499 7400 7662 820
Size: 197 926 3550 1006 901 262 1553
Fragments arranged by size:
3550 1553 1006 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5692 6186 493
Size: 5199 494 2702
Fragments arranged by size:
5199 2702 494
SpeI A'CTAG_T
Cuts at: 152 3242 5681 152
Size: 3090 2439 2866
Fragments arranged by size:
3090 2866 2439
SphI G_CATG'C
Cuts at: 1132 5753 1132
Size: 4621 3774
SspI AAT'ATT
Cuts at: 5 52 4182 5018 6319 5
Size: 47 4130 836 1301 2081
Fragments arranged by size:
4130 2081 1301 836 47
StuI AGG'CCT
Cuts at: 1517 3284 1517
Size: 1767 6628
StyI C'CwwG_G
Cuts at: 513 1065 2022 2619 2988 3444 4113 4369 4455
Size: 552 957 597 369 456 669 256 86
Cuts at: 4455 4717 5474 5834 513
Size: 262 757 360 3074
Fragments arranged by size:
3074 957 757 669 597 552 456 369
360 262 256 86
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2453 2938 3049 3913 4523 5224 6422
Size: 1255 873 485 111 864 610 701 1198
Cuts at: 6422 6439 6592 6777 7222 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1198 873 864 701 610 485
445 185 153 111 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3309 3341 3846
Size: 274 80 33 51 1443 1332 32 505
Cuts at: 3846 4446 6641 8353 96
Size: 600 2195 1712 138
Fragments arranged by size:
2195 1712 1443 1332 600 505 274 138
80 51 33 32
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3621 3653 3729 3761 3958 3990 4265 4297 4301
Size: 32 76 32 197 32 275 32 4
Cuts at: 4301 4333 8355 8387 3621
Size: 32 4022 32 3629
Fragments arranged by size:
4022 3629 275 197 76 32 32 32
32 32 32 4
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8395
XbaI T'CTAG_A
Cuts at: 5731 5731
Size: 8395
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4469 4601 4967 4469
Size: 132 366 7897
Fragments arranged by size:
7897 366 132
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8395
XmaI C'CCGG_G
Cuts at: 1870 2514 5720 1870
Size: 644 3206 4545
Fragments arranged by size:
4545 3206 644
XmnI GAAnn'nnTTC
Cuts at: 4181 6524 4181
Size: 2343 6052
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6203 276
Size: 53 83 186 936 4669 2468
Fragments arranged by size:
4669 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BsiWI
BsmBI BstZ17I EcoRV FseI FspAI NruI PacI PspOMI
PspXI PsrI RsrII SfiI SrfI SwaI