Restriction map for
pFN21ASDA1056
(Circular) (Six-base) MAPSORT of: pFN21ASDA1056.seq Check: 752 from: 1 to: 7315
pFN21ASDA1056 7315 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4048 4101 4404 4491 4048
Size: 53 303 87 6872
Fragments arranged by size:
6872 303 87 53
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3558 5125 278
Size: 53 83 186 936 2022 1567 2468
Fragments arranged by size:
2468 2022 1567 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4658 1937
Size: 2721 4594
Acc65I G'GTAC_C
Cuts at: 2054 4636 2054
Size: 2582 4733
AclI AA'CG_TT
Cuts at: 1563 5442 5815 1563
Size: 3879 373 3063
Fragments arranged by size:
3879 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4186 4190 5378 6762 7030 1632
Size: 2554 4 1188 1384 268 1917
Fragments arranged by size:
2554 1917 1384 1188 268 4
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7118
AflIII A'CryG_T
Cuts at: 1203 3342 6214 7191 1203
Size: 2139 2872 977 1327
Fragments arranged by size:
2872 2139 1327 977
AgeI A'CCGG_T
Cuts at: 6146 6146
Size: 7315
AhdI GACnn_n'nnGTC
Cuts at: 4162 4507 6044 4162
Size: 345 1537 5433
Fragments arranged by size:
5433 1537 345
AleI CACnn'nnGTG
Cuts at: 1166 3382 1166
Size: 2216 5099
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3617 4554 1184
Size: 2433 937 3945
Fragments arranged by size:
3945 2433 937
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2162 2194 2162
Size: 32 7283
AlwNI CAG_nnn'CTG
Cuts at: 2203 2833 3308 3424 3468 3477 3832 4111 4592
Size: 630 475 116 44 9 355 279 481
Cuts at: 4592 5058 6630 2203
Size: 466 1572 2888
Fragments arranged by size:
2888 1572 630 481 475 466 355 279
116 44 9
ApaI G_GGCC'C
Cuts at: 3650 3933 3650
Size: 283 7032
ApaLI G'TGCA_C
Cuts at: 3431 5372 6528 3431
Size: 1941 1156 4218
Fragments arranged by size:
4218 1941 1156
ApoI r'AATT_y
Cuts at: 1493 2580 4624 4850 5002 7111 1493
Size: 1087 2044 226 152 2109 1697
Fragments arranged by size:
2109 2044 1697 1087 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5869 160
Size: 872 4837 1606
Fragments arranged by size:
4837 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7315
AvaI C'yCGr_G
Cuts at: 1870 1943 3970 4640 1870
Size: 73 2027 670 4545
Fragments arranged by size:
4545 2027 670 73
AvrII C'CTAG_G
Cuts at: 3940 3940
Size: 7315
BamHI G'GATC_C
Cuts at: 4645 5012 4645
Size: 367 6948
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2054 3491 4319 4421 4636
Size: 325 217 627 267 1437 828 102 215
Cuts at: 4636 6091 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1437 828 627 325 267 217
215 102
BanII G_rGCy'C
Cuts at: 1391 2250 2337 2481 3177 3245 3650 3933 4456
Size: 859 87 144 696 68 405 283 523
Cuts at: 4456 4634 1391
Size: 178 4072
Fragments arranged by size:
4072 859 696 523 405 283 178 144
87 68
BbeI G_GCGC'C
Cuts at: 3495 3495
Size: 7315
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2719 3258 928
Size: 971 820 539 4985
Fragments arranged by size:
4985 971 820 539
BbvCI CC'TCA_GC
Cuts at: 2599 3050 3326 3590 3977 2599
Size: 451 276 264 387 5937
Fragments arranged by size:
5937 451 387 276 264
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5506 5540 1903
Size: 34 3569 34 3678
Fragments arranged by size:
3678 3569 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5207 6417 5207
Size: 1210 6105
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7315
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5926 136
Size: 107 122 71 631 605 4254 1525
Fragments arranged by size:
4254 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7310 7310
Size: 7315
BlpI GC'TnA_GC
Cuts at: 4732 4732
Size: 7315
Bme1580I G_kGCm'C
Cuts at: 1790 2076 3224 3435 3650 3933 4424 5376 6532
Size: 286 1148 211 215 283 491 952 1156
Cuts at: 6532 1790
Size: 2573
Fragments arranged by size:
2573 1156 1148 952 491 286 283 215
211
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7315
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2475 3230 3252
Size: 416 53 83 399 24 1058 755 22
Cuts at: 3252 5046 6004 442
Size: 1794 958 1753
Fragments arranged by size:
1794 1753 1058 958 755 416 399 83
53 24 22
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7315
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2095 2225 2397 2665 2840 2918 4238 5975
Size: 856 130 172 268 175 78 1320 1737
Cuts at: 5975 1239
Size: 2579
Fragments arranged by size:
2579 1737 1320 856 268 175 172 130
78
Bpu10I CC'TnA_GC
Cuts at: 2351 2599 3050 3326 3590 3977 2351
Size: 248 451 276 264 387 5689
Fragments arranged by size:
5689 451 387 276 264 248
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3365 4313 4337 5440 6305 6603 3365
Size: 948 24 1103 865 298 4077
Fragments arranged by size:
4077 1103 948 865 298 24
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5978 882
Size: 617 4479 2219
Fragments arranged by size:
4479 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2719 4030 4612 5106 493
Size: 711 1515 1311 582 494 2702
Fragments arranged by size:
2702 1515 1311 711 582 494
BsaBI GATnn'nnATC
Cuts at: 7309 7309
Size: 7315
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3492 3555 5122 5504
Size: 53 83 186 936 1959 63 1567 382
Cuts at: 5504 6139 275
Size: 635 1451
Fragments arranged by size:
1959 1567 1451 936 635 382 186 83
63 53
BsaWI w'CCGG_w
Cuts at: 5748 6146 6420 6567 6978 5748
Size: 398 274 147 411 6085
Fragments arranged by size:
6085 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2090 2120 1178
Size: 30 165 30 687 30 6373
Fragments arranged by size:
6373 687 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2098 2146 2333 3864 1186
Size: 912 48 187 1531 4637
Fragments arranged by size:
4637 1531 912 187 48
BseYI C'CCAG_C
Cuts at: 2845 3086 3270 3301 3485 3838 4002 4323 4432
Size: 241 184 31 184 353 164 321 109
Cuts at: 4432 6518 7014 2845
Size: 2086 496 3146
Fragments arranged by size:
3146 2086 496 353 321 241 184 184
164 109 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3763 3806 4569 1163
Size: 573 2027 43 763 3909
Fragments arranged by size:
3909 2027 763 573 43
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4765 5526 6554 1540
Size: 275 186 2764 761 1028 2301
Fragments arranged by size:
2764 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3435 4039 5376 5461 6532 3435
Size: 604 1337 85 1071 4218
Fragments arranged by size:
4218 1337 1071 604 85
BsmI GAATG_Cn'
Cuts at: 4826 4919 4826
Size: 93 7222
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2076 2250 2337 2481 3177 3224 3245
Size: 399 286 174 87 144 696 47 21
Cuts at: 3245 3435 3650 3933 4039 4424 4456 4634 5376
Size: 190 215 283 106 385 32 178 742
Cuts at: 5376 5461 6532 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 696 399 385 286 283
215 190 178 174 144 106 87 85
47 32 21
BspEI T'CCGG_A
Cuts at: 6978 6978
Size: 7315
BspHI T'CATG_A
Cuts at: 5204 6934 5204
Size: 1730 5585
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3780 4048 4101 4404 4491 4670 844
Size: 2936 268 53 303 87 179 3489
Fragments arranged by size:
3489 2936 303 268 179 87 53
BsrBI CCG'CTC
Cuts at: 1394 1823 5202 1394
Size: 429 3379 3507
Fragments arranged by size:
3507 3379 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5810 5984 65
Size: 1183 4562 174 1396
Fragments arranged by size:
4562 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5959 6146 1700
Size: 110 4149 187 2869
Fragments arranged by size:
4149 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7315
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7315
BssSI C'ACGA_G
Cuts at: 3510 5375 6387 3510
Size: 1865 1012 4438
Fragments arranged by size:
4438 1865 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3369 3477 3741 4308 794
Size: 2575 108 264 567 3801
Fragments arranged by size:
3801 2575 567 264 108
BstBI TT'CG_AA
Cuts at: 2007 6119 2007
Size: 4112 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7315
BstXI CCAn_nnnn'nTGG
Cuts at: 3926 3926
Size: 7315
BstYI r'GATC_y
Cuts at: 1972 2140 2677 4645 5012 5397 5414 6866 6952
Size: 168 537 1968 367 385 17 1452 86
Cuts at: 6952 6964 7310 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1968 1452 537 385 367 346 168
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3000 3150 3000
Size: 150 7165
BtgI C'CryG_G
Cuts at: 513 1065 2022 2343 2694 3693 4416 513
Size: 552 957 321 351 999 723 3412
Fragments arranged by size:
3412 999 957 723 552 351 321
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6576
Fragments arranged by size:
6576 683 56
BtsI GCAGTG_nn'
Cuts at: 2428 2520 3466 3487 3737 4234 4831 5625 5645
Size: 92 946 21 250 497 597 794 20
Cuts at: 5645 2428
Size: 4098
Fragments arranged by size:
4098 946 794 597 497 250 92 21
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7315
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3837 3872 529
Size: 35 3273 35 3972
Fragments arranged by size:
3972 3273 35 35
DraI TTT'AAA
Cuts at: 4619 4619
Size: 7315
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6322 809
Size: 507 5006 1802
Fragments arranged by size:
5006 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2025 2397 3621
Size: 54 1425 50 275 18 195 372 1224
Cuts at: 3621 4762 5651 7280 8
Size: 1141 889 1629 43
Fragments arranged by size:
1629 1425 1224 1141 889 372 275 195
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4762 1537
Size: 275 2950 4090
Fragments arranged by size:
4090 2950 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2469 2575 2929 3156 3920 5245 1353
Size: 1116 106 354 227 764 1325 3423
Fragments arranged by size:
3423 1325 1116 764 354 227 106
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4051 5902 6276 6422 387
Size: 1064 138 2462 1851 374 146 1280
Fragments arranged by size:
2462 1851 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2095 2225 2397 2665 2840 2918 4186
Size: 393 463 130 172 268 175 78 1268
Cuts at: 4186 4190 4238 5378 5975 6762 7030 1239
Size: 4 48 1140 597 787 268 1524
Fragments arranged by size:
1524 1268 1140 787 597 463 393 268
268 175 172 130 78 48 4
EcoNI CCTnn'n_nnAGG
Cuts at: 4400 4400
Size: 7315
EcoO109I rG'GnC_Cy
Cuts at: 2178 2258 2830 2881 3016 3082 3322 3647 3869
Size: 80 572 51 135 66 240 325 222
Cuts at: 3869 3930 3937 2178
Size: 61 7 5556
Fragments arranged by size:
5556 572 325 240 222 135 80 66
61 51 7
EcoRI G'AATT_C
Cuts at: 4624 5002 4624
Size: 378 6937
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4354 4354
Size: 7315
FspI TGC'GCA
Cuts at: 3776 5821 3776
Size: 2045 5270
HaeII r_GCGC'y
Cuts at: 1678 3495 6462 1678
Size: 1817 2967 2531
Fragments arranged by size:
2967 2531 1817
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2090 2122 3448 3480 4148
Size: 32 135 32 518 32 1326 32 668
Cuts at: 4148 4180 4493 4525 5956 5988 6030 6062 1373
Size: 32 313 32 1431 32 42 32 2626
Fragments arranged by size:
2626 1431 1326 668 518 313 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4659 4905 669
Size: 988 281 2721 246 3079
Fragments arranged by size:
3079 2721 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2890 4304 748
Size: 2142 1414 3759
Fragments arranged by size:
3759 2142 1414
HpaI GTT'AAC
Cuts at: 4905 4905
Size: 7315
KasI G'GCGC_C
Cuts at: 3491 3491
Size: 7315
KpnI G_GTAC'C
Cuts at: 2058 4640 2058
Size: 2582 4733
MfeI C'AATT_G
Cuts at: 4914 4914
Size: 7315
MluI A'CGCG_T
Cuts at: 7191 7191
Size: 7315
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2560 3692 4366 4536 6429 6613 1305
Size: 1255 1132 674 170 1893 184 2007
Fragments arranged by size:
2007 1893 1255 1132 674 184 170
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2027 2399 3623 10
Size: 54 1425 343 195 372 1224 3702
Fragments arranged by size:
3702 1425 1224 372 343 195 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3382 3599 3782 5273 5632 5791 518
Size: 648 2216 217 183 1491 359 159 2042
Fragments arranged by size:
2216 2042 1491 648 359 217 183 159
MspA1I CmG'CkG
Cuts at: 1606 2375 2656 2706 2849 3549 3695 3838 4182
Size: 769 281 50 143 700 146 143 344
Cuts at: 4182 4299 4384 4534 4731 5408 6556 6801 1606
Size: 117 85 150 197 677 1148 245 2120
Fragments arranged by size:
2120 1148 769 700 677 344 281 245
197 150 146 143 143 117 85 50
NarI GG'CG_CC
Cuts at: 3492 3492
Size: 7315
NcoI C'CATG_G
Cuts at: 513 1065 2022 2343 2694 4416 513
Size: 552 957 321 351 1722 3412
Fragments arranged by size:
3412 1722 957 552 351 321
NdeI CA'TA_TG
Cuts at: 387 3600 3783 387
Size: 3213 183 3919
Fragments arranged by size:
3919 3213 183
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7315
NotI GC'GGCC_GC
Cuts at: 4762 4762
Size: 7315
NspI r_CATG'y
Cuts at: 1132 1229 3346 3439 3805 4370 4541 4673 6218
Size: 97 2117 93 366 565 171 132 1545
Cuts at: 6218 1132
Size: 2229
Fragments arranged by size:
2229 2117 1545 565 366 171 132 97
93
PasI CC'CwG_GG
Cuts at: 1348 2069 2291 2451 4441 1348
Size: 721 222 160 1990 4222
Fragments arranged by size:
4222 1990 721 222 160
PciI A'CATG_T
Cuts at: 3342 6214 3342
Size: 2872 4443
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7315
PfoI T'CCnGG_A
Cuts at: 2202 2856 3027 4257 2202
Size: 654 171 1230 5260
Fragments arranged by size:
5260 1230 654 171
PmeI GTTT'AAAC
Cuts at: 4619 4619
Size: 7315
PmlI CAC'GTG
Cuts at: 2719 2719
Size: 7315
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5384 5416 6897 6929 5384
Size: 32 1481 32 5770
Fragments arranged by size:
5770 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2178 3082 2178
Size: 904 6411
PshAI GACnn'nnGTC
Cuts at: 1613 2268 1613
Size: 655 6660
PsiI TTA'TAA
Cuts at: 4885 4885
Size: 7315
PspOMI G'GGCC_C
Cuts at: 3646 3929 3646
Size: 283 7032
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2418 2450 2418
Size: 32 7283
PstI C_TGCA'G
Cuts at: 830 3313 3473 3482 4241 4667 830
Size: 2483 160 9 759 426 3478
Fragments arranged by size:
3478 2483 759 426 160 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7315
PvuII CAG'CTG
Cuts at: 2375 2656 2849 4384 4534 2375
Size: 281 193 1535 150 5156
Fragments arranged by size:
5156 1535 281 193 150
SacII CC_GC'GG
Cuts at: 3696 3696
Size: 7315
SalI G'TCGA_C
Cuts at: 1936 4657 1936
Size: 2721 4594
SanDI GG'GwC_CC
Cuts at: 3082 3082
Size: 7315
SapI GCTCTTCn'nnn_
Cuts at: 2469 2575 2469
Size: 106 7209
SbfI CC_TGCA'GG
Cuts at: 4667 4667
Size: 7315
ScaI AGT'ACT
Cuts at: 3923 5563 7275 3923
Size: 1640 1712 3963
Fragments arranged by size:
3963 1712 1640
SfcI C'TryA_G
Cuts at: 826 1045 3309 3469 3478 3629 4237 4663 5798
Size: 219 2264 160 9 151 608 426 1135
Cuts at: 5798 6479 6670 826
Size: 681 191 1471
Fragments arranged by size:
2264 1471 1135 681 608 426 219 191
160 151 9
SfoI GGC'GCC
Cuts at: 3493 3493
Size: 7315
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7315
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7315
SmaI CCC'GGG
Cuts at: 1872 3972 4642 1872
Size: 2100 670 4545
Fragments arranged by size:
4545 2100 670
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3380 4328 4352 5419 6320 6582
Size: 197 926 1437 948 24 1067 901 262
Cuts at: 6582 820
Size: 1553
Fragments arranged by size:
1553 1437 1067 948 926 901 262 197
24
SnaBI TAC'GTA
Cuts at: 493 4612 5106 493
Size: 4119 494 2702
Fragments arranged by size:
4119 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7315
SphI G_CATG'C
Cuts at: 1132 4541 4673 1132
Size: 3409 132 3774
Fragments arranged by size:
3774 3409 132
SspI AAT'ATT
Cuts at: 5 52 5239 5
Size: 47 5187 2081
Fragments arranged by size:
5187 2081 47
StuI AGG'CCT
Cuts at: 1517 2387 2774 4199 1517
Size: 870 387 1425 4633
Fragments arranged by size:
4633 1425 870 387
StyI C'CwwG_G
Cuts at: 513 1065 2022 2343 2557 2694 3210 3940 3960
Size: 552 957 321 214 137 516 730 20
Cuts at: 3960 4416 4754 513
Size: 456 338 3074
Fragments arranged by size:
3074 957 730 552 516 456 338 321
214 137 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3082 3217 4307 4337 5342 5359 5512
Size: 1255 1502 135 1090 30 1005 17 153
Cuts at: 5512 5697 6142 325
Size: 185 445 1498
Fragments arranged by size:
1502 1498 1255 1090 1005 445 185 153
135 30 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2851 3921 5561
Size: 274 80 33 51 1443 874 1070 1640
Cuts at: 5561 7273 96
Size: 1712 138
Fragments arranged by size:
1712 1640 1443 1070 874 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2125 2157 7275 7307 2125
Size: 32 5118 32 2133
Fragments arranged by size:
5118 2133 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7315
XbaI T'CTAG_A
Cuts at: 4651 4651
Size: 7315
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2987 3647 2987
Size: 660 6655
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7315
XmaI C'CCGG_G
Cuts at: 1870 3970 4640 1870
Size: 2100 670 4545
Fragments arranged by size:
4545 2100 670
XmnI GAAnn'nnTTC
Cuts at: 4175 5444 4175
Size: 1269 6046
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3556 5123 276
Size: 53 83 186 936 2022 1567 2468
Fragments arranged by size:
2468 2022 1567 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacII
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BfrBI BplI BsiWI BsmBI BstZ17I
DraIII EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI
NsiI PacI PspXI RsrII SacI SexAI SfiI SrfI
SwaI