Restriction map for
pFN21ASDA1044
(Circular) (Six-base) MAPSORT of: pFN21ASDA1044.seq Check: 3490 from: 1 to: 6619
pFN21ASDA1044 6619 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3471 3614 3471
Size: 143 6476
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2877 4429 278
Size: 53 83 186 936 1341 1552 2468
Fragments arranged by size:
2468 1552 1341 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3110 3679 3962 1937
Size: 1173 569 283 4594
Fragments arranged by size:
4594 1173 569 283
Acc65I G'GTAC_C
Cuts at: 3940 3940
Size: 6619
AclI AA'CG_TT
Cuts at: 1563 2203 4746 5119 1563
Size: 640 2543 373 3063
Fragments arranged by size:
3063 2543 640 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2899 2993 3232 4682 6066 6334 1632
Size: 1267 94 239 1450 1384 268 1917
Fragments arranged by size:
1917 1450 1384 1267 268 239 94
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6422
AflIII A'CryG_T
Cuts at: 1203 5518 6495 1203
Size: 4315 977 1327
Fragments arranged by size:
4315 1327 977
AgeI A'CCGG_T
Cuts at: 5450 5450
Size: 6619
AhdI GACnn_n'nnGTC
Cuts at: 5348 5348
Size: 6619
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6619
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6619
AlwNI CAG_nnn'CTG
Cuts at: 3606 4362 5934 3606
Size: 756 1572 4291
Fragments arranged by size:
4291 1572 756
ApaLI G'TGCA_C
Cuts at: 4676 5832 4676
Size: 1156 5463
ApoI r'AATT_y
Cuts at: 1493 2311 3928 4154 4306 6415 1493
Size: 818 1617 226 152 2109 1697
Fragments arranged by size:
2109 1697 1617 818 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5173 160
Size: 872 4141 1606
Fragments arranged by size:
4141 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6619
AvaI C'yCGr_G
Cuts at: 1870 1943 2092 2113 3944 1870
Size: 73 149 21 1831 4545
Fragments arranged by size:
4545 1831 149 73 21
BamHI G'GATC_C
Cuts at: 3318 3949 4316 3318
Size: 631 367 5621
Fragments arranged by size:
5621 631 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2168 3151 3940 5395 618
Size: 325 217 627 381 983 789 1455 1842
Fragments arranged by size:
1842 1455 983 789 627 381 325 217
BanII G_rGCy'C
Cuts at: 1391 3938 1391
Size: 2547 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2896 2905 3060 3573 928
Size: 971 997 9 155 513 3974
Fragments arranged by size:
3974 997 971 513 155 9
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4810 4844 1903
Size: 34 2873 34 3678
Fragments arranged by size:
3678 2873 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2473 3250 4511 5721 2473
Size: 777 1261 1210 3371
Fragments arranged by size:
3371 1261 1210 777
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6619
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5230 136
Size: 107 122 71 631 605 3558 1525
Fragments arranged by size:
3558 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6614 6614
Size: 6619
BlpI GC'TnA_GC
Cuts at: 4036 4036
Size: 6619
Bme1580I G_kGCm'C
Cuts at: 1790 3295 4680 5836 1790
Size: 1505 1385 1156 2573
Fragments arranged by size:
2573 1505 1385 1156
BmgBI CAC'GTC
Cuts at: 1318 2184 2821 1318
Size: 866 637 5116
Fragments arranged by size:
5116 866 637
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2231 2334 4350
Size: 416 53 83 399 24 814 103 2016
Cuts at: 4350 5308 442
Size: 958 1753
Fragments arranged by size:
2016 1753 958 814 416 399 103 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3078 1056
Size: 2022 4597
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2374 3741 2374
Size: 1367 5252
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2557 3421 3816 5279 1239
Size: 1318 864 395 1463 2579
Fragments arranged by size:
2579 1463 1318 864 395
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2928 4744 5609 5907 2928
Size: 1816 865 298 3640
Fragments arranged by size:
3640 1816 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5282 882
Size: 617 3783 2219
Fragments arranged by size:
3783 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3916 4410 493
Size: 711 2712 494 2702
Fragments arranged by size:
2712 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 3701 6613 3701
Size: 2912 3707
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2874 4426 4808 5443
Size: 53 83 186 936 1341 1552 382 635
Cuts at: 5443 275
Size: 1451
Fragments arranged by size:
1552 1451 1341 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3229 5052 5450 5724 5871 6282 3229
Size: 1823 398 274 147 411 3566
Fragments arranged by size:
3566 1823 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2874 2904 1178
Size: 30 165 30 1471 30 4893
Fragments arranged by size:
4893 1471 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2441 1186
Size: 1255 5364
BseYI C'CCAG_C
Cuts at: 2492 3450 5822 6318 2492
Size: 958 2372 496 2793
Fragments arranged by size:
2793 2372 958 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6046
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4069 4830 5858 1540
Size: 275 186 2068 761 1028 2301
Fragments arranged by size:
2301 2068 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4680 4765 5836 4680
Size: 85 1071 5463
Fragments arranged by size:
5463 1071 85
BsmI GAATG_Cn'
Cuts at: 4130 4223 4130
Size: 93 6526
BsmBI CGTCTCn'nnnn_
Cuts at: 2617 3846 2617
Size: 1229 5390
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3295 3938 4680 4765 5836 1391
Size: 399 1505 643 742 85 1071 2174
Fragments arranged by size:
2174 1505 1071 742 643 399 85
BspEI T'CCGG_A
Cuts at: 6282 6282
Size: 6619
BspHI T'CATG_A
Cuts at: 2733 2805 4508 6238 2733
Size: 72 1703 1730 3114
Fragments arranged by size:
3114 1730 1703 72
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3471 3614 3974 844
Size: 2627 143 360 3489
Fragments arranged by size:
3489 2627 360 143
BsrBI CCG'CTC
Cuts at: 1394 1823 2692 4506 1394
Size: 429 869 1814 3507
Fragments arranged by size:
3507 1814 869 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2604 3213 5114 5288 65
Size: 1183 1356 609 1901 174 1396
Fragments arranged by size:
1901 1396 1356 1183 609 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2104 5263 5450 1700
Size: 110 294 3159 187 2869
Fragments arranged by size:
3159 2869 294 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6619
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6619
BssSI C'ACGA_G
Cuts at: 2348 3494 4679 5691 2348
Size: 1146 1185 1012 3276
Fragments arranged by size:
3276 1185 1146 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6619
BstBI TT'CG_AA
Cuts at: 2007 5423 2007
Size: 3416 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6619
BstXI CCAn_nnnn'nTGG
Cuts at: 2569 2569
Size: 6619
BstYI r'GATC_y
Cuts at: 1972 2919 3318 3949 4316 4701 4718 6170 6256
Size: 947 399 631 367 385 17 1452 86
Cuts at: 6256 6268 6614 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 947 631 399 385 367 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 3111 3680 3111
Size: 569 6050
BtgI C'CryG_G
Cuts at: 513 1065 2022 3018 513
Size: 552 957 996 4114
Fragments arranged by size:
4114 996 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2605 487
Size: 683 56 1379 4501
Fragments arranged by size:
4501 1379 683 56
BtsI GCAGTG_nn'
Cuts at: 3469 4135 4929 4949 3469
Size: 666 794 20 5139
Fragments arranged by size:
5139 794 666 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6619
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6584
DraI TTT'AAA
Cuts at: 3750 3923 3750
Size: 173 6446
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3512 5626 809
Size: 507 2196 2114 1802
Fragments arranged by size:
2196 2114 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2826 3312 4066
Size: 54 1425 50 275 18 996 486 754
Cuts at: 4066 4955 6584 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 996 889 754 486 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4066 1537
Size: 275 2254 4090
Fragments arranged by size:
4090 2254 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2969 3759 4549 1353
Size: 1616 790 790 3423
Fragments arranged by size:
3423 1616 790 790
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2288 5206 5580 5726 387
Size: 1064 138 699 2918 374 146 1280
Fragments arranged by size:
2918 1280 1064 699 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2557 2899 2993 3232 3421 3816 4682
Size: 393 925 342 94 239 189 395 866
Cuts at: 4682 5279 6066 6334 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 925 866 787 597 395 393 342
268 239 189 94
EcoO109I rG'GnC_Cy
Cuts at: 2096 2133 2541 2096
Size: 37 408 6174
Fragments arranged by size:
6174 408 37
EcoRI G'AATT_C
Cuts at: 3928 4306 3928
Size: 378 6241
FspI TGC'GCA
Cuts at: 2954 5125 2954
Size: 2171 4448
HaeII r_GCGC'y
Cuts at: 1678 2464 2505 2777 5766 1678
Size: 786 41 272 2989 2531
Fragments arranged by size:
2989 2531 786 272 41
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2874 2906 3050 3082 5260
Size: 32 135 32 1302 32 144 32 2178
Cuts at: 5260 5292 5334 5366 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2178 1302 144 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3963 4209 669
Size: 988 281 2025 246 3079
Fragments arranged by size:
3079 2025 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6619
HpaI GTT'AAC
Cuts at: 4209 4209
Size: 6619
KpnI G_GTAC'C
Cuts at: 3944 3944
Size: 6619
MfeI C'AATT_G
Cuts at: 4218 4218
Size: 6619
MluI A'CGCG_T
Cuts at: 6495 6495
Size: 6619
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3267 5733 5917 1305
Size: 1962 2466 184 2007
Fragments arranged by size:
2466 2007 1962 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2828 3314 10
Size: 54 1425 343 996 486 3315
Fragments arranged by size:
3315 1425 996 486 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2819 4577 4936 5095 518
Size: 648 1653 1758 359 159 2042
Fragments arranged by size:
2042 1758 1653 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2066 2492 2880 3391 4035 4712 5860 6105
Size: 460 426 388 511 644 677 1148 245
Cuts at: 6105 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 644 511 460 426 388
245
NaeI GCC'GGC
Cuts at: 2106 2106
Size: 6619
NcoI C'CATG_G
Cuts at: 513 1065 2022 3018 513
Size: 552 957 996 4114
Fragments arranged by size:
4114 996 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6619
NgoMIV G'CCGG_C
Cuts at: 2104 2104
Size: 6619
NheI G'CTAG_C
Cuts at: 1052 3074 1052
Size: 2022 4597
NotI GC'GGCC_GC
Cuts at: 4066 4066
Size: 6619
NruI TCG'CGA
Cuts at: 2624 2624
Size: 6619
NspI r_CATG'y
Cuts at: 1132 1229 3358 3977 5522 1132
Size: 97 2129 619 1545 2229
Fragments arranged by size:
2229 2129 1545 619 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6619
PciI A'CATG_T
Cuts at: 5518 5518
Size: 6619
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6619
PfoI T'CCnGG_A
Cuts at: 2397 2397
Size: 6619
PmeI GTTT'AAAC
Cuts at: 3923 3923
Size: 6619
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4688 4720 6201 6233 4688
Size: 32 1481 32 5074
Fragments arranged by size:
5074 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2133 2133
Size: 6619
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6619
PsiI TTA'TAA
Cuts at: 4189 4189
Size: 6619
PstI C_TGCA'G
Cuts at: 830 2469 2773 3100 3396 3971 830
Size: 1639 304 327 296 575 3478
Fragments arranged by size:
3478 1639 575 327 304 296
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6619
PvuII CAG'CTG
Cuts at: 3391 3391
Size: 6619
SalI G'TCGA_C
Cuts at: 1936 3961 1936
Size: 2025 4594
SbfI CC_TGCA'GG
Cuts at: 2469 3971 2469
Size: 1502 5117
ScaI AGT'ACT
Cuts at: 3878 4867 6579 3878
Size: 989 1712 3918
Fragments arranged by size:
3918 1712 989
SfcI C'TryA_G
Cuts at: 826 1045 2465 2769 3039 3096 3392 3804 3967
Size: 219 1420 304 270 57 296 412 163
Cuts at: 3967 5102 5783 5974 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1420 1135 681 412 304 296 270
219 191 163 57
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6619
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6619
SmaI CCC'GGG
Cuts at: 1872 3946 1872
Size: 2074 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2943 4723 5624 5886 820
Size: 197 926 1000 1780 901 262 1553
Fragments arranged by size:
1780 1553 1000 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3916 4410 493
Size: 3423 494 2702
Fragments arranged by size:
3423 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6619
SphI G_CATG'C
Cuts at: 1132 3977 1132
Size: 2845 3774
SspI AAT'ATT
Cuts at: 5 52 3892 4543 5
Size: 47 3840 651 2081
Fragments arranged by size:
3840 2081 651 47
StuI AGG'CCT
Cuts at: 1517 2949 1517
Size: 1432 5187
StyI C'CwwG_G
Cuts at: 513 1065 2022 3018 4058 513
Size: 552 957 996 1040 3074
Fragments arranged by size:
3074 1040 996 957 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2268 4646 4663 4816 5001 5446 325
Size: 1255 688 2378 17 153 185 445 1498
Fragments arranged by size:
2378 1498 1255 688 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2430 3876 4865
Size: 274 80 33 51 1443 453 1446 989
Cuts at: 4865 6577 96
Size: 1712 138
Fragments arranged by size:
1712 1446 1443 989 453 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6579 6611 6579
Size: 32 6587
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6619
XbaI T'CTAG_A
Cuts at: 3955 3955
Size: 6619
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6619
XmaI C'CCGG_G
Cuts at: 1870 3944 1870
Size: 2074 4545
XmnI GAAnn'nnTTC
Cuts at: 4748 4748
Size: 6619
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2875 4427 276
Size: 53 83 186 936 1341 1552 2468
Fragments arranged by size:
2468 1552 1341 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NruI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NarI NsiI PacI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI