(Circular) (Six-base) MAPSORT of: pFN21ASDA1044.seq Check: 3490 from: 1 to: 6619 pFN21ASDA1044 6619 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3471 3614 3471 Size: 143 6476 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2877 4429 278 Size: 53 83 186 936 1341 1552 2468 Fragments arranged by size: 2468 1552 1341 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3110 3679 3962 1937 Size: 1173 569 283 4594 Fragments arranged by size: 4594 1173 569 283 Acc65I G'GTAC_C Cuts at: 3940 3940 Size: 6619 AclI AA'CG_TT Cuts at: 1563 2203 4746 5119 1563 Size: 640 2543 373 3063 Fragments arranged by size: 3063 2543 640 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2899 2993 3232 4682 6066 6334 1632 Size: 1267 94 239 1450 1384 268 1917 Fragments arranged by size: 1917 1450 1384 1267 268 239 94 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6422 AflIII A'CryG_T Cuts at: 1203 5518 6495 1203 Size: 4315 977 1327 Fragments arranged by size: 4315 1327 977 AgeI A'CCGG_T Cuts at: 5450 5450 Size: 6619 AhdI GACnn_n'nnGTC Cuts at: 5348 5348 Size: 6619 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6619 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6619 AlwNI CAG_nnn'CTG Cuts at: 3606 4362 5934 3606 Size: 756 1572 4291 Fragments arranged by size: 4291 1572 756 ApaLI G'TGCA_C Cuts at: 4676 5832 4676 Size: 1156 5463 ApoI r'AATT_y Cuts at: 1493 2311 3928 4154 4306 6415 1493 Size: 818 1617 226 152 2109 1697 Fragments arranged by size: 2109 1697 1617 818 226 152 AseI AT'TA_AT Cuts at: 160 1032 5173 160 Size: 872 4141 1606 Fragments arranged by size: 4141 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6619 AvaI C'yCGr_G Cuts at: 1870 1943 2092 2113 3944 1870 Size: 73 149 21 1831 4545 Fragments arranged by size: 4545 1831 149 73 21 BamHI G'GATC_C Cuts at: 3318 3949 4316 3318 Size: 631 367 5621 Fragments arranged by size: 5621 631 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2168 3151 3940 5395 618 Size: 325 217 627 381 983 789 1455 1842 Fragments arranged by size: 1842 1455 983 789 627 381 325 217 BanII G_rGCy'C Cuts at: 1391 3938 1391 Size: 2547 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2896 2905 3060 3573 928 Size: 971 997 9 155 513 3974 Fragments arranged by size: 3974 997 971 513 155 9 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4810 4844 1903 Size: 34 2873 34 3678 Fragments arranged by size: 3678 2873 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2473 3250 4511 5721 2473 Size: 777 1261 1210 3371 Fragments arranged by size: 3371 1261 1210 777 BclI T'GATC_A Cuts at: 1548 1548 Size: 6619 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5230 136 Size: 107 122 71 631 605 3558 1525 Fragments arranged by size: 3558 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6614 6614 Size: 6619 BlpI GC'TnA_GC Cuts at: 4036 4036 Size: 6619 Bme1580I G_kGCm'C Cuts at: 1790 3295 4680 5836 1790 Size: 1505 1385 1156 2573 Fragments arranged by size: 2573 1505 1385 1156 BmgBI CAC'GTC Cuts at: 1318 2184 2821 1318 Size: 866 637 5116 Fragments arranged by size: 5116 866 637 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2231 2334 4350 Size: 416 53 83 399 24 814 103 2016 Cuts at: 4350 5308 442 Size: 958 1753 Fragments arranged by size: 2016 1753 958 814 416 399 103 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3078 1056 Size: 2022 4597 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2374 3741 2374 Size: 1367 5252 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2557 3421 3816 5279 1239 Size: 1318 864 395 1463 2579 Fragments arranged by size: 2579 1463 1318 864 395 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2928 4744 5609 5907 2928 Size: 1816 865 298 3640 Fragments arranged by size: 3640 1816 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5282 882 Size: 617 3783 2219 Fragments arranged by size: 3783 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3916 4410 493 Size: 711 2712 494 2702 Fragments arranged by size: 2712 2702 711 494 BsaBI GATnn'nnATC Cuts at: 3701 6613 3701 Size: 2912 3707 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2874 4426 4808 5443 Size: 53 83 186 936 1341 1552 382 635 Cuts at: 5443 275 Size: 1451 Fragments arranged by size: 1552 1451 1341 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3229 5052 5450 5724 5871 6282 3229 Size: 1823 398 274 147 411 3566 Fragments arranged by size: 3566 1823 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2874 2904 1178 Size: 30 165 30 1471 30 4893 Fragments arranged by size: 4893 1471 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2441 1186 Size: 1255 5364 BseYI C'CCAG_C Cuts at: 2492 3450 5822 6318 2492 Size: 958 2372 496 2793 Fragments arranged by size: 2793 2372 958 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6046 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4069 4830 5858 1540 Size: 275 186 2068 761 1028 2301 Fragments arranged by size: 2301 2068 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4680 4765 5836 4680 Size: 85 1071 5463 Fragments arranged by size: 5463 1071 85 BsmI GAATG_Cn' Cuts at: 4130 4223 4130 Size: 93 6526 BsmBI CGTCTCn'nnnn_ Cuts at: 2617 3846 2617 Size: 1229 5390 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3295 3938 4680 4765 5836 1391 Size: 399 1505 643 742 85 1071 2174 Fragments arranged by size: 2174 1505 1071 742 643 399 85 BspEI T'CCGG_A Cuts at: 6282 6282 Size: 6619 BspHI T'CATG_A Cuts at: 2733 2805 4508 6238 2733 Size: 72 1703 1730 3114 Fragments arranged by size: 3114 1730 1703 72 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3471 3614 3974 844 Size: 2627 143 360 3489 Fragments arranged by size: 3489 2627 360 143 BsrBI CCG'CTC Cuts at: 1394 1823 2692 4506 1394 Size: 429 869 1814 3507 Fragments arranged by size: 3507 1814 869 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2604 3213 5114 5288 65 Size: 1183 1356 609 1901 174 1396 Fragments arranged by size: 1901 1396 1356 1183 609 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2104 5263 5450 1700 Size: 110 294 3159 187 2869 Fragments arranged by size: 3159 2869 294 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6619 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6619 BssSI C'ACGA_G Cuts at: 2348 3494 4679 5691 2348 Size: 1146 1185 1012 3276 Fragments arranged by size: 3276 1185 1146 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6619 BstBI TT'CG_AA Cuts at: 2007 5423 2007 Size: 3416 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6619 BstXI CCAn_nnnn'nTGG Cuts at: 2569 2569 Size: 6619 BstYI r'GATC_y Cuts at: 1972 2919 3318 3949 4316 4701 4718 6170 6256 Size: 947 399 631 367 385 17 1452 86 Cuts at: 6256 6268 6614 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 947 631 399 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3111 3680 3111 Size: 569 6050 BtgI C'CryG_G Cuts at: 513 1065 2022 3018 513 Size: 552 957 996 4114 Fragments arranged by size: 4114 996 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2605 487 Size: 683 56 1379 4501 Fragments arranged by size: 4501 1379 683 56 BtsI GCAGTG_nn' Cuts at: 3469 4135 4929 4949 3469 Size: 666 794 20 5139 Fragments arranged by size: 5139 794 666 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6619 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6584 DraI TTT'AAA Cuts at: 3750 3923 3750 Size: 173 6446 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3512 5626 809 Size: 507 2196 2114 1802 Fragments arranged by size: 2196 2114 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2826 3312 4066 Size: 54 1425 50 275 18 996 486 754 Cuts at: 4066 4955 6584 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 996 889 754 486 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4066 1537 Size: 275 2254 4090 Fragments arranged by size: 4090 2254 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2969 3759 4549 1353 Size: 1616 790 790 3423 Fragments arranged by size: 3423 1616 790 790 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2288 5206 5580 5726 387 Size: 1064 138 699 2918 374 146 1280 Fragments arranged by size: 2918 1280 1064 699 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2557 2899 2993 3232 3421 3816 4682 Size: 393 925 342 94 239 189 395 866 Cuts at: 4682 5279 6066 6334 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 925 866 787 597 395 393 342 268 239 189 94 EcoO109I rG'GnC_Cy Cuts at: 2096 2133 2541 2096 Size: 37 408 6174 Fragments arranged by size: 6174 408 37 EcoRI G'AATT_C Cuts at: 3928 4306 3928 Size: 378 6241 FspI TGC'GCA Cuts at: 2954 5125 2954 Size: 2171 4448 HaeII r_GCGC'y Cuts at: 1678 2464 2505 2777 5766 1678 Size: 786 41 272 2989 2531 Fragments arranged by size: 2989 2531 786 272 41 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2874 2906 3050 3082 5260 Size: 32 135 32 1302 32 144 32 2178 Cuts at: 5260 5292 5334 5366 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2178 1302 144 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3963 4209 669 Size: 988 281 2025 246 3079 Fragments arranged by size: 3079 2025 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6619 HpaI GTT'AAC Cuts at: 4209 4209 Size: 6619 KpnI G_GTAC'C Cuts at: 3944 3944 Size: 6619 MfeI C'AATT_G Cuts at: 4218 4218 Size: 6619 MluI A'CGCG_T Cuts at: 6495 6495 Size: 6619 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3267 5733 5917 1305 Size: 1962 2466 184 2007 Fragments arranged by size: 2466 2007 1962 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2828 3314 10 Size: 54 1425 343 996 486 3315 Fragments arranged by size: 3315 1425 996 486 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2819 4577 4936 5095 518 Size: 648 1653 1758 359 159 2042 Fragments arranged by size: 2042 1758 1653 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2066 2492 2880 3391 4035 4712 5860 6105 Size: 460 426 388 511 644 677 1148 245 Cuts at: 6105 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 644 511 460 426 388 245 NaeI GCC'GGC Cuts at: 2106 2106 Size: 6619 NcoI C'CATG_G Cuts at: 513 1065 2022 3018 513 Size: 552 957 996 4114 Fragments arranged by size: 4114 996 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6619 NgoMIV G'CCGG_C Cuts at: 2104 2104 Size: 6619 NheI G'CTAG_C Cuts at: 1052 3074 1052 Size: 2022 4597 NotI GC'GGCC_GC Cuts at: 4066 4066 Size: 6619 NruI TCG'CGA Cuts at: 2624 2624 Size: 6619 NspI r_CATG'y Cuts at: 1132 1229 3358 3977 5522 1132 Size: 97 2129 619 1545 2229 Fragments arranged by size: 2229 2129 1545 619 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6619 PciI A'CATG_T Cuts at: 5518 5518 Size: 6619 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6619 PfoI T'CCnGG_A Cuts at: 2397 2397 Size: 6619 PmeI GTTT'AAAC Cuts at: 3923 3923 Size: 6619 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4688 4720 6201 6233 4688 Size: 32 1481 32 5074 Fragments arranged by size: 5074 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2133 2133 Size: 6619 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6619 PsiI TTA'TAA Cuts at: 4189 4189 Size: 6619 PstI C_TGCA'G Cuts at: 830 2469 2773 3100 3396 3971 830 Size: 1639 304 327 296 575 3478 Fragments arranged by size: 3478 1639 575 327 304 296 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6619 PvuII CAG'CTG Cuts at: 3391 3391 Size: 6619 SalI G'TCGA_C Cuts at: 1936 3961 1936 Size: 2025 4594 SbfI CC_TGCA'GG Cuts at: 2469 3971 2469 Size: 1502 5117 ScaI AGT'ACT Cuts at: 3878 4867 6579 3878 Size: 989 1712 3918 Fragments arranged by size: 3918 1712 989 SfcI C'TryA_G Cuts at: 826 1045 2465 2769 3039 3096 3392 3804 3967 Size: 219 1420 304 270 57 296 412 163 Cuts at: 3967 5102 5783 5974 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1420 1135 681 412 304 296 270 219 191 163 57 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6619 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6619 SmaI CCC'GGG Cuts at: 1872 3946 1872 Size: 2074 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2943 4723 5624 5886 820 Size: 197 926 1000 1780 901 262 1553 Fragments arranged by size: 1780 1553 1000 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3916 4410 493 Size: 3423 494 2702 Fragments arranged by size: 3423 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6619 SphI G_CATG'C Cuts at: 1132 3977 1132 Size: 2845 3774 SspI AAT'ATT Cuts at: 5 52 3892 4543 5 Size: 47 3840 651 2081 Fragments arranged by size: 3840 2081 651 47 StuI AGG'CCT Cuts at: 1517 2949 1517 Size: 1432 5187 StyI C'CwwG_G Cuts at: 513 1065 2022 3018 4058 513 Size: 552 957 996 1040 3074 Fragments arranged by size: 3074 1040 996 957 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2268 4646 4663 4816 5001 5446 325 Size: 1255 688 2378 17 153 185 445 1498 Fragments arranged by size: 2378 1498 1255 688 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2430 3876 4865 Size: 274 80 33 51 1443 453 1446 989 Cuts at: 4865 6577 96 Size: 1712 138 Fragments arranged by size: 1712 1446 1443 989 453 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6579 6611 6579 Size: 32 6587 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6619 XbaI T'CTAG_A Cuts at: 3955 3955 Size: 6619 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6619 XmaI C'CCGG_G Cuts at: 1870 3944 1870 Size: 2074 4545 XmnI GAAnn'nnTTC Cuts at: 4748 4748 Size: 6619 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2875 4427 276 Size: 53 83 186 936 1341 1552 2468 Fragments arranged by size: 2468 1552 1341 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI