Restriction map for
pFN21ASDA1034
(Circular) (Six-base) MAPSORT of: pFN21ASDA1034.seq Check: 6912 from: 1 to: 6928
pFN21ASDA1034 6928 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4210 4210
Size: 6928
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2091 4738 278
Size: 53 83 186 936 555 2647 2468
Fragments arranged by size:
2647 2468 936 555 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4271 1937
Size: 2334 4594
Acc65I G'GTAC_C
Cuts at: 4023 4249 4023
Size: 226 6702
AclI AA'CG_TT
Cuts at: 1563 5055 5428 1563
Size: 3492 373 3063
Fragments arranged by size:
3492 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3158 3864 4991 6375 6643 1632
Size: 1526 706 1127 1384 268 1917
Fragments arranged by size:
1917 1526 1384 1127 706 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6731
AflIII A'CryG_T
Cuts at: 1203 3730 5827 6804 1203
Size: 2527 2097 977 1327
Fragments arranged by size:
2527 2097 1327 977
AgeI A'CCGG_T
Cuts at: 5759 5759
Size: 6928
AhdI GACnn_n'nnGTC
Cuts at: 5657 5657
Size: 6928
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6928
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2692 1184
Size: 1508 5420
AlwNI CAG_nnn'CTG
Cuts at: 3040 4202 4671 6243 3040
Size: 1162 469 1572 3725
Fragments arranged by size:
3725 1572 1162 469
ApaLI G'TGCA_C
Cuts at: 4985 6141 4985
Size: 1156 5772
ApoI r'AATT_y
Cuts at: 1493 2847 3280 4237 4463 4615 6724 1493
Size: 1354 433 957 226 152 2109 1697
Fragments arranged by size:
2109 1697 1354 957 433 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5482 160
Size: 872 4450 1606
Fragments arranged by size:
4450 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6928
AvaI C'yCGr_G
Cuts at: 1870 1943 4253 1870
Size: 73 2310 4545
Fragments arranged by size:
4545 2310 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3622 3655 3622
Size: 33 6895
BamHI G'GATC_C
Cuts at: 3903 4258 4625 3903
Size: 355 367 6206
Fragments arranged by size:
6206 367 355
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2168 3455 4023 4249 5704
Size: 325 217 627 381 1287 568 226 1455
Cuts at: 5704 618
Size: 1842
Fragments arranged by size:
1842 1455 1287 627 568 381 325 226
217
BanII G_rGCy'C
Cuts at: 1391 2045 2152 2906 4067 4247 1391
Size: 654 107 754 1161 180 4072
Fragments arranged by size:
4072 1161 754 654 180 107
BbeI G_GCGC'C
Cuts at: 2172 3459 2172
Size: 1287 5641
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2517 2903 3234 3852 928
Size: 971 618 386 331 618 4004
Fragments arranged by size:
4004 971 618 618 386 331
BbvCI CC'TCA_GC
Cuts at: 4167 4167
Size: 6928
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5119 5153 1903
Size: 34 3182 34 3678
Fragments arranged by size:
3678 3182 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4820 6030 4820
Size: 1210 5718
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6928
BfrBI ATG'CAT
Cuts at: 2782 2782
Size: 6928
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3545 5539 136
Size: 107 122 71 631 605 1873 1994 1525
Fragments arranged by size:
1994 1873 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3882 6923 3882
Size: 3041 3887
BlpI GC'TnA_GC
Cuts at: 3972 4345 3972
Size: 373 6555
Bme1580I G_kGCm'C
Cuts at: 1790 2883 4989 6145 1790
Size: 1093 2106 1156 2573
Fragments arranged by size:
2573 2106 1156 1093
BmgBI CAC'GTC
Cuts at: 1318 4078 1318
Size: 2760 4168
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2099 4659 5617
Size: 416 53 83 399 24 682 2560 958
Cuts at: 5617 442
Size: 1753
Fragments arranged by size:
2560 1753 958 682 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6928
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3822 4156 3822
Size: 334 6594
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2144 3090 3361 5588 1239
Size: 905 946 271 2227 2579
Fragments arranged by size:
2579 2227 946 905 271
Bpu10I CC'TnA_GC
Cuts at: 3750 4167 3750
Size: 417 6511
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3003 3510 5053 5918 6216 3003
Size: 507 1543 865 298 3715
Fragments arranged by size:
3715 1543 865 507 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3370 3876 5591 882
Size: 617 1871 506 1715 2219
Fragments arranged by size:
2219 1871 1715 617 506
BsaAI yAC'GTr
Cuts at: 493 1204 3733 4030 4225 4719 493
Size: 711 2529 297 195 494 2702
Fragments arranged by size:
2702 2529 711 494 297 195
BsaBI GATnn'nnATC
Cuts at: 2460 6922 2460
Size: 4462 2466
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2088 2169 3456 4735
Size: 53 83 186 936 555 81 1287 1279
Cuts at: 4735 5117 5752 275
Size: 382 635 1451
Fragments arranged by size:
1451 1287 1279 936 635 555 382 186
83 81 53
BsaWI w'CCGG_w
Cuts at: 5361 5759 6033 6180 6591 5361
Size: 398 274 147 411 5698
Fragments arranged by size:
5698 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4049 4079 1178
Size: 30 165 30 2646 30 4027
Fragments arranged by size:
4027 2646 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2826 3358 3382 3707 3710 3825 4112 4133
Size: 1640 532 24 325 3 115 287 21
Cuts at: 4133 4160 4184 1186
Size: 27 24 3930
Fragments arranged by size:
3930 1640 532 325 287 115 27 24
24 21 3
BseYI C'CCAG_C
Cuts at: 2380 2922 3386 3666 6131 6627 2380
Size: 542 464 280 2465 496 2681
Fragments arranged by size:
2681 2465 542 496 464 280
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3779 1163
Size: 573 2043 4312
Fragments arranged by size:
4312 2043 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2068 4378 5139 6167 1540
Size: 275 186 67 2310 761 1028 2301
Fragments arranged by size:
2310 2301 1028 761 275 186 67
BsiHKAI G_wGCw'C
Cuts at: 2591 4059 4989 5074 6145 2591
Size: 1468 930 85 1071 3374
Fragments arranged by size:
3374 1468 1071 930 85
BsmI GAATG_Cn'
Cuts at: 2609 3179 4439 4532 2609
Size: 570 1260 93 5005
Fragments arranged by size:
5005 1260 570 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2045 2152 2591 2883 2906 4059 4067
Size: 399 255 107 439 292 23 1153 8
Cuts at: 4067 4247 4989 5074 6145 1391
Size: 180 742 85 1071 2174
Fragments arranged by size:
2174 1153 1071 742 439 399 292 255
180 107 85 23 8
BspEI T'CCGG_A
Cuts at: 6591 6591
Size: 6928
BspHI T'CATG_A
Cuts at: 2898 3922 4817 6547 2898
Size: 1024 895 1730 3279
Fragments arranged by size:
3279 1730 1024 895
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3751 4210 4283 844
Size: 2907 459 73 3489
Fragments arranged by size:
3489 2907 459 73
BsrBI CCG'CTC
Cuts at: 1394 1823 2029 2076 3337 4815 1394
Size: 429 206 47 1261 1478 3507
Fragments arranged by size:
3507 1478 1261 429 206 47
BsrDI GCAATG_nn'
Cuts at: 65 1248 2557 5423 5597 65
Size: 1183 1309 2866 174 1396
Fragments arranged by size:
2866 1396 1309 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2067 2994 3754 5572 5759 1700
Size: 110 257 927 760 1818 187 2869
Fragments arranged by size:
2869 1818 927 760 257 187 110
BsrGI T'GTAC_A
Cuts at: 96 4152 96
Size: 4056 2872
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6928
BssSI C'ACGA_G
Cuts at: 4988 6000 4988
Size: 1012 5916
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4202 794
Size: 3408 3520
BstBI TT'CG_AA
Cuts at: 2007 5732 2007
Size: 3725 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6928
BstXI CCAn_nnnn'nTGG
Cuts at: 2185 2687 2185
Size: 502 6426
BstYI r'GATC_y
Cuts at: 1972 3492 3882 3903 3979 4258 4625 5010 5027
Size: 1520 390 21 76 279 367 385 17
Cuts at: 5027 6479 6565 6577 6923 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1520 1452 390 385 367 346 279
86 76 21 17 12
Bsu36I CC'TnA_GG
Cuts at: 3266 3405 3266
Size: 139 6789
BtgI C'CryG_G
Cuts at: 513 1065 2022 2142 2152 2172 2184 2958 3093
Size: 552 957 120 10 20 12 774 135
Cuts at: 3093 4039 4068 513
Size: 946 29 3373
Fragments arranged by size:
3373 957 946 774 552 135 120 29
20 12 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6189
Fragments arranged by size:
6189 683 56
BtsI GCAGTG_nn'
Cuts at: 2534 4444 5238 5258 2534
Size: 1910 794 20 4204
Fragments arranged by size:
4204 1910 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6928
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6893
DraI TTT'AAA
Cuts at: 4232 4232
Size: 6928
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5935 809
Size: 507 4619 1802
Fragments arranged by size:
4619 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2175 2439 4375
Size: 54 1425 50 275 18 345 264 1936
Cuts at: 4375 5264 6893 8
Size: 889 1629 43
Fragments arranged by size:
1936 1629 1425 889 345 275 264 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4375 1537
Size: 275 2563 4090
Fragments arranged by size:
4090 2563 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3094 3134 3258 4858 1353
Size: 1741 40 124 1600 3423
Fragments arranged by size:
3423 1741 1600 124 40
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2047 2086 5515 5889 6035 387
Size: 1064 138 458 39 3429 374 146 1280
Fragments arranged by size:
3429 1280 1064 458 374 146 138 39
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2144 3090 3158 3361 3864 4991 5588
Size: 393 512 946 68 203 503 1127 597
Cuts at: 5588 6375 6643 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1127 946 787 597 512 503 393
268 203 68
EcoO109I rG'GnC_Cy
Cuts at: 2096 2096
Size: 6928
EcoRI G'AATT_C
Cuts at: 4237 4615 4237
Size: 378 6550
EcoRV GAT'ATC
Cuts at: 3112 3112
Size: 6928
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2312 3904 2312
Size: 1592 5336
FspI TGC'GCA
Cuts at: 5434 5434
Size: 6928
HaeII r_GCGC'y
Cuts at: 1678 2172 3459 3824 6075 1678
Size: 494 1287 365 2251 2531
Fragments arranged by size:
2531 2251 1287 494 365
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2032 2064 2893 2925 3229
Size: 32 135 32 460 32 829 32 304
Cuts at: 3229 3261 3300 3331 3332 3363 3683 3715 5569
Size: 32 39 31 1 31 320 32 1854
Cuts at: 5569 5601 5643 5675 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1854 829 460 320 304 135 42
39 32 32 32 32 32 32 32
32 31 31 1
HincII GTy'rAC
Cuts at: 669 1657 1938 4272 4518 669
Size: 988 281 2334 246 3079
Fragments arranged by size:
3079 2334 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3913 748
Size: 3165 3763
HpaI GTT'AAC
Cuts at: 4518 4518
Size: 6928
KasI G'GCGC_C
Cuts at: 2168 3455 2168
Size: 1287 5641
KpnI G_GTAC'C
Cuts at: 4027 4253 4027
Size: 226 6702
MfeI C'AATT_G
Cuts at: 4527 4527
Size: 6928
MluI A'CGCG_T
Cuts at: 6804 6804
Size: 6928
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2208 2487 3520 3863 6042 6226 1305
Size: 903 279 1033 343 2179 184 2007
Fragments arranged by size:
2179 2007 1033 903 343 279 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2177 10
Size: 54 1425 343 345 4761
Fragments arranged by size:
4761 1425 345 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4886 5245 5404 518
Size: 648 3720 359 159 2042
Fragments arranged by size:
3720 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2154 2308 2803 3476 3588 3976 4021 4070
Size: 548 154 495 673 112 388 45 49
Cuts at: 4070 4344 5021 6169 6414 1606
Size: 274 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 673 548 495 388 274
245 154 112 49 45
NaeI GCC'GGC
Cuts at: 2996 2996
Size: 6928
NarI GG'CG_CC
Cuts at: 2169 3456 2169
Size: 1287 5641
NcoI C'CATG_G
Cuts at: 513 1065 2022 2142 2958 513
Size: 552 957 120 816 4483
Fragments arranged by size:
4483 957 816 552 120
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6928
NgoMIV G'CCGG_C
Cuts at: 2994 2994
Size: 6928
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6928
NotI GC'GGCC_GC
Cuts at: 4375 4375
Size: 6928
NsiI A_TGCA'T
Cuts at: 2784 2784
Size: 6928
NspI r_CATG'y
Cuts at: 1132 1229 2449 3727 4286 5831 1132
Size: 97 1220 1278 559 1545 2229
Fragments arranged by size:
2229 1545 1278 1220 559 97
PasI CC'CwG_GG
Cuts at: 1348 2332 2812 3898 1348
Size: 984 480 1086 4378
Fragments arranged by size:
4378 1086 984 480
PciI A'CATG_T
Cuts at: 5827 5827
Size: 6928
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2311 1108
Size: 1203 5725
PmeI GTTT'AAAC
Cuts at: 4232 4232
Size: 6928
PmlI CAC'GTG
Cuts at: 3733 4030 3733
Size: 297 6631
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4997 5029 6510 6542 4997
Size: 32 1481 32 5383
Fragments arranged by size:
5383 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3778 1613
Size: 2165 4763
PsiI TTA'TAA
Cuts at: 4498 4498
Size: 6928
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4007 4039 4007
Size: 32 6896
PstI C_TGCA'G
Cuts at: 830 4280 830
Size: 3450 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6928
PvuII CAG'CTG
Cuts at: 2308 2803 3476 3976 2308
Size: 495 673 500 5260
Fragments arranged by size:
5260 673 500 495
SacII CC_GC'GG
Cuts at: 2155 4071 2155
Size: 1916 5012
SalI G'TCGA_C
Cuts at: 1936 4270 1936
Size: 2334 4594
SbfI CC_TGCA'GG
Cuts at: 4280 4280
Size: 6928
ScaI AGT'ACT
Cuts at: 2860 5176 6888 2860
Size: 2316 1712 2900
Fragments arranged by size:
2900 2316 1712
SfcI C'TryA_G
Cuts at: 826 1045 4276 5411 6092 6283 826
Size: 219 3231 1135 681 191 1471
Fragments arranged by size:
3231 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2170 3457 2170
Size: 1287 5641
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6928
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6928
SmaI CCC'GGG
Cuts at: 1872 4255 1872
Size: 2383 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3018 3525 5032 5933 6195 820
Size: 197 926 1075 507 1507 901 262 1553
Fragments arranged by size:
1553 1507 1075 926 901 507 262 197
SnaBI TAC'GTA
Cuts at: 493 4225 4719 493
Size: 3732 494 2702
Fragments arranged by size:
3732 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6928
SphI G_CATG'C
Cuts at: 1132 3727 4286 1132
Size: 2595 559 3774
Fragments arranged by size:
3774 2595 559
SspI AAT'ATT
Cuts at: 5 52 4852 5
Size: 47 4800 2081
Fragments arranged by size:
4800 2081 47
StuI AGG'CCT
Cuts at: 1517 3933 1517
Size: 2416 4512
StyI C'CwwG_G
Cuts at: 513 1065 2022 2142 2178 2365 2958 3516 3792
Size: 552 957 120 36 187 593 558 276
Cuts at: 3792 4367 513
Size: 575 3074
Fragments arranged by size:
3074 957 593 575 558 552 276 187
120 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3067 4124 4955 4972 5125 5310 5755
Size: 1255 1487 1057 831 17 153 185 445
Cuts at: 5755 325
Size: 1498
Fragments arranged by size:
1498 1487 1255 1057 831 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2706 2858 4152
Size: 274 80 33 51 1443 729 152 1294
Cuts at: 4152 5174 6886 96
Size: 1022 1712 138
Fragments arranged by size:
1712 1443 1294 1022 729 274 152 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4049 4081 6888 6920 4049
Size: 32 2807 32 4057
Fragments arranged by size:
4057 2807 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4111 1316
Size: 2795 4133
XbaI T'CTAG_A
Cuts at: 4264 4264
Size: 6928
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2170 3091 3358 2170
Size: 921 267 5740
Fragments arranged by size:
5740 921 267
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6928
XmaI C'CCGG_G
Cuts at: 1870 4253 1870
Size: 2383 4545
XmnI GAAnn'nnTTC
Cuts at: 5057 5057
Size: 6928
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2089 4736 276
Size: 53 83 186 936 555 2647 2468
Fragments arranged by size:
2647 2468 936 555 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PsrI PstI PvuI PvuII SacII
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BsiWI BsmBI BstZ17I
DraIII EcoICRI EcoNI FseI FspAI NruI PacI PfoI
PpuMI PspOMI PspXI RsrII SacI SanDI SapI SexAI
SfiI SrfI SwaI