Restriction map for
pFN21ASDA1028
(Circular) (Six-base) MAPSORT of: pFN21ASDA1028.seq Check: 5712 from: 1 to: 6235
pFN21ASDA1028 6235 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3239 3239
Size: 6235
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4045 278
Size: 53 83 186 936 2509 2468
Fragments arranged by size:
2509 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2127 3578 1937
Size: 190 1451 4594
Fragments arranged by size:
4594 1451 190
Acc65I G'GTAC_C
Cuts at: 3556 3556
Size: 6235
AclI AA'CG_TT
Cuts at: 1563 4362 4735 1563
Size: 2799 373 3063
Fragments arranged by size:
3063 2799 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2942 3135 3339 4298 5682 5950 1632
Size: 1310 193 204 959 1384 268 1917
Fragments arranged by size:
1917 1384 1310 959 268 204 193
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6038
AflIII A'CryG_T
Cuts at: 1203 5134 6111 1203
Size: 3931 977 1327
Fragments arranged by size:
3931 1327 977
AgeI A'CCGG_T
Cuts at: 3522 5066 3522
Size: 1544 4691
AhdI GACnn_n'nnGTC
Cuts at: 4964 4964
Size: 6235
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6235
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6235
AlwNI CAG_nnn'CTG
Cuts at: 3978 5550 3978
Size: 1572 4663
ApaLI G'TGCA_C
Cuts at: 4292 5448 4292
Size: 1156 5079
ApoI r'AATT_y
Cuts at: 1493 3092 3544 3770 3922 6031 1493
Size: 1599 452 226 152 2109 1697
Fragments arranged by size:
2109 1697 1599 452 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4789 160
Size: 872 3757 1606
Fragments arranged by size:
3757 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6235
AvaI C'yCGr_G
Cuts at: 1870 1943 3560 1870
Size: 73 1617 4545
Fragments arranged by size:
4545 1617 73
BamHI G'GATC_C
Cuts at: 2593 2977 3016 3440 3565 3932 2593
Size: 384 39 424 125 367 4896
Fragments arranged by size:
4896 424 384 367 125 39
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2111 3205 3275 3322 3556
Size: 325 217 627 324 1094 70 47 234
Cuts at: 3556 5011 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1094 627 325 324 234 217
70 47
BanII G_rGCy'C
Cuts at: 1391 2626 3494 3554 1391
Size: 1235 868 60 4072
Fragments arranged by size:
4072 1235 868 60
BbeI G_GCGC'C
Cuts at: 3209 3209
Size: 6235
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5264
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4426 4460 1903
Size: 34 2489 34 3678
Fragments arranged by size:
3678 2489 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2447 4127 5337 2447
Size: 1680 1210 3345
Fragments arranged by size:
3345 1680 1210
BclI T'GATC_A
Cuts at: 1548 2770 1548
Size: 1222 5013
BfrBI ATG'CAT
Cuts at: 2171 2171
Size: 6235
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4846 136
Size: 107 122 71 631 605 3174 1525
Fragments arranged by size:
3174 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2396 6230 2396
Size: 3834 2401
BlpI GC'TnA_GC
Cuts at: 3652 3652
Size: 6235
Bme1580I G_kGCm'C
Cuts at: 1790 3278 4296 5452 1790
Size: 1488 1018 1156 2573
Fragments arranged by size:
2573 1488 1156 1018
BmgBI CAC'GTC
Cuts at: 1318 2125 1318
Size: 807 5428
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2830 3966 4924
Size: 416 53 83 399 24 1413 1136 958
Cuts at: 4924 442
Size: 1753
Fragments arranged by size:
1753 1413 1136 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6235
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2610 3292 3388 4895 1239
Size: 1371 682 96 1507 2579
Fragments arranged by size:
2579 1507 1371 682 96
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4360 5225 5523 4360
Size: 865 298 5072
Fragments arranged by size:
5072 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4898 882
Size: 617 3399 2219
Fragments arranged by size:
3399 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3532 4026 493
Size: 711 2328 494 2702
Fragments arranged by size:
2702 2328 711 494
BsaBI GATnn'nnATC
Cuts at: 2592 6229 2592
Size: 3637 2598
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3206 4042 4424 5059
Size: 53 83 186 936 1673 836 382 635
Cuts at: 5059 275
Size: 1451
Fragments arranged by size:
1673 1451 936 836 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3278 3522 4668 5066 5340 5487 5898 3278
Size: 244 1146 398 274 147 411 3615
Fragments arranged by size:
3615 1146 411 398 274 244 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2063 2093 3160 3190 1178
Size: 30 165 30 660 30 1067 30 4223
Fragments arranged by size:
4223 1067 660 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2559 1186
Size: 1373 4862
BseYI C'CCAG_C
Cuts at: 3257 5438 5934 3257
Size: 2181 496 3558
Fragments arranged by size:
3558 2181 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5662
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3685 4446 5474 1540
Size: 275 186 1684 761 1028 2301
Fragments arranged by size:
2301 1684 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3461 3494 4296 4381 5452 3461
Size: 33 802 85 1071 4244
Fragments arranged by size:
4244 1071 802 85 33
BsmI GAATG_Cn'
Cuts at: 3746 3839 3746
Size: 93 6142
BsmBI CGTCTCn'nnnn_
Cuts at: 3486 3486
Size: 6235
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2626 3278 3461 3494 3554 4296 4381
Size: 399 836 652 183 33 60 742 85
Cuts at: 4381 5452 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 836 742 652 399 183 85
60 33
BspEI T'CCGG_A
Cuts at: 5898 5898
Size: 6235
BspHI T'CATG_A
Cuts at: 2767 4124 5854 2767
Size: 1357 1730 3148
Fragments arranged by size:
3148 1730 1357
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3239 3590 844
Size: 2395 351 3489
Fragments arranged by size:
3489 2395 351
BsrBI CCG'CTC
Cuts at: 1394 1823 2059 2065 4122 1394
Size: 429 236 6 2057 3507
Fragments arranged by size:
3507 2057 429 236 6
BsrDI GCAATG_nn'
Cuts at: 65 1248 2261 4730 4904 65
Size: 1183 1013 2469 174 1396
Fragments arranged by size:
2469 1396 1183 1013 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3062 3522 4879 5066 1700
Size: 110 1252 460 1357 187 2869
Fragments arranged by size:
2869 1357 1252 460 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6235
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6235
BssSI C'ACGA_G
Cuts at: 4295 5307 4295
Size: 1012 5223
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3010 794
Size: 2216 4019
BstBI TT'CG_AA
Cuts at: 2007 5039 2007
Size: 3032 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6235
BstYI r'GATC_y
Cuts at: 1972 2396 2593 2977 3016 3440 3565 3932 4317
Size: 424 197 384 39 424 125 367 385
Cuts at: 4317 4334 5786 5872 5884 6230 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 424 424 385 384 367 346
197 125 86 39 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2973 3341 513
Size: 552 957 951 368 3407
Fragments arranged by size:
3407 957 951 552 368
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5496
Fragments arranged by size:
5496 683 56
BtsI GCAGTG_nn'
Cuts at: 3751 4545 4565 3751
Size: 794 20 5421
Fragments arranged by size:
5421 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6235
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6200
DraI TTT'AAA
Cuts at: 3539 3539
Size: 6235
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5242 809
Size: 507 3926 1802
Fragments arranged by size:
3926 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3682 4571 6200
Size: 54 1425 50 275 18 1852 889 1629
Cuts at: 6200 8
Size: 43
Fragments arranged by size:
1852 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3682 1537
Size: 275 1870 4090
Fragments arranged by size:
4090 1870 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3478 4165 1353
Size: 2125 687 3423
Fragments arranged by size:
3423 2125 687
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2069 2155 4822 5196 5342 387
Size: 1064 138 480 86 2667 374 146 1280
Fragments arranged by size:
2667 1280 1064 480 374 146 138 86
EcoICRI GAG'CTC
Cuts at: 3492 3492
Size: 6235
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2610 2942 3135 3292 3339 3388 4298
Size: 393 978 332 193 157 47 49 910
Cuts at: 4298 4895 5682 5950 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 978 910 787 597 393 332 268
193 157 49 47
EcoNI CCTnn'n_nnAGG
Cuts at: 3317 3317
Size: 6235
EcoO109I rG'GnC_Cy
Cuts at: 2510 3034 3350 2510
Size: 524 316 5395
Fragments arranged by size:
5395 524 316
EcoRI G'AATT_C
Cuts at: 3092 3544 3922 3092
Size: 452 378 5405
Fragments arranged by size:
5405 452 378
FspI TGC'GCA
Cuts at: 4741 4741
Size: 6235
HaeII r_GCGC'y
Cuts at: 1678 3209 5382 1678
Size: 1531 2173 2531
Fragments arranged by size:
2531 2173 1531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2962 2994 4876 4908 4950
Size: 32 135 32 1390 32 1882 32 42
Cuts at: 4950 4982 1373
Size: 32 2626
Fragments arranged by size:
2626 1882 1390 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2706 3579 3825 669
Size: 988 281 768 873 246 3079
Fragments arranged by size:
3079 988 873 768 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6235
HpaI GTT'AAC
Cuts at: 3825 3825
Size: 6235
KasI G'GCGC_C
Cuts at: 3205 3205
Size: 6235
KpnI G_GTAC'C
Cuts at: 3560 3560
Size: 6235
MfeI C'AATT_G
Cuts at: 3834 3834
Size: 6235
MluI A'CGCG_T
Cuts at: 6111 6111
Size: 6235
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3005 5349 5533 1305
Size: 1700 2344 184 2007
Fragments arranged by size:
2344 2007 1700 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4413
Fragments arranged by size:
4413 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2250 4193 4552 4711 518
Size: 648 1084 1943 359 159 2042
Fragments arranged by size:
2042 1943 1084 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3651 4328 5476 5721 1606
Size: 2045 677 1148 245 2120
Fragments arranged by size:
2120 2045 1148 677 245
NaeI GCC'GGC
Cuts at: 3064 3064
Size: 6235
NarI GG'CG_CC
Cuts at: 3206 3206
Size: 6235
NcoI C'CATG_G
Cuts at: 513 1065 2022 2973 513
Size: 552 957 951 3775
Fragments arranged by size:
3775 957 951 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6235
NgoMIV G'CCGG_C
Cuts at: 3062 3062
Size: 6235
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6235
NotI GC'GGCC_GC
Cuts at: 3682 3682
Size: 6235
NsiI A_TGCA'T
Cuts at: 2173 2173
Size: 6235
NspI r_CATG'y
Cuts at: 1132 1229 3593 5138 1132
Size: 97 2364 1545 2229
Fragments arranged by size:
2364 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6235
PciI A'CATG_T
Cuts at: 5134 5134
Size: 6235
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6235
PmeI GTTT'AAAC
Cuts at: 3539 3539
Size: 6235
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4304 4336 5817 5849 4304
Size: 32 1481 32 4690
Fragments arranged by size:
4690 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2510 3034 2510
Size: 524 5711
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6235
PsiI TTA'TAA
Cuts at: 3805 3805
Size: 6235
PstI C_TGCA'G
Cuts at: 830 2812 3015 3587 830
Size: 1982 203 572 3478
Fragments arranged by size:
3478 1982 572 203
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6235
SacI G_AGCT'C
Cuts at: 3494 3494
Size: 6235
SalI G'TCGA_C
Cuts at: 1936 3577 1936
Size: 1641 4594
SbfI CC_TGCA'GG
Cuts at: 3587 3587
Size: 6235
ScaI AGT'ACT
Cuts at: 4483 6195 4483
Size: 1712 4523
SexAI A'CCwGG_T
Cuts at: 3318 3318
Size: 6235
SfcI C'TryA_G
Cuts at: 826 1045 2808 3011 3583 4718 5399 5590 826
Size: 219 1763 203 572 1135 681 191 1471
Fragments arranged by size:
1763 1471 1135 681 572 219 203 191
SfoI GGC'GCC
Cuts at: 3207 3207
Size: 6235
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6235
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6235
SmaI CCC'GGG
Cuts at: 1872 3562 1872
Size: 1690 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4339 5240 5502 820
Size: 197 926 2396 901 262 1553
Fragments arranged by size:
2396 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3532 4026 493
Size: 3039 494 2702
Fragments arranged by size:
3039 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6235
SphI G_CATG'C
Cuts at: 1132 3593 1132
Size: 2461 3774
SspI AAT'ATT
Cuts at: 5 52 4159 5
Size: 47 4107 2081
Fragments arranged by size:
4107 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6235
StyI C'CwwG_G
Cuts at: 513 1065 2022 2973 3674 513
Size: 552 957 951 701 3074
Fragments arranged by size:
3074 957 951 701 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2449 3245 3519 4262 4279 4432 4617
Size: 1255 869 796 274 743 17 153 185
Cuts at: 4617 5062 325
Size: 445 1498
Fragments arranged by size:
1498 1255 869 796 743 445 274 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2907 4481 6193
Size: 274 80 33 51 1443 930 1574 1712
Cuts at: 6193 96
Size: 138
Fragments arranged by size:
1712 1574 1443 930 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2776 2808 3160 3192 3235 3267 6195 6227 2776
Size: 32 352 32 43 32 2928 32 2784
Fragments arranged by size:
2928 2784 352 43 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6235
XbaI T'CTAG_A
Cuts at: 3571 3571
Size: 6235
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2429 3461 2429
Size: 1032 5203
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6235
XmaI C'CCGG_G
Cuts at: 1870 3560 1870
Size: 1690 4545
XmnI GAAnn'nnTTC
Cuts at: 2273 4364 2273
Size: 2091 4144
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4043 276
Size: 53 83 186 936 2509 2468
Fragments arranged by size:
2509 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BplI
Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI
FseI FspAI NruI PacI PfoI PmlI PspOMI PspXI
PsrI PvuII RsrII SacII SanDI SapI SfiI SrfI
SwaI