Restriction map for
pFN21ASDA1027
(Circular) (Six-base) MAPSORT of: pFN21ASDA1027.seq Check: 4307 from: 1 to: 12352
pFN21ASDA1027 12352 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5387 8527 5387
Size: 3140 9212
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5450 10162 278
Size: 53 83 186 936 3914 4712 2468
Fragments arranged by size:
4712 3914 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2087 3272 7036 8910 9695 1937
Size: 150 1185 3764 1874 785 4594
Fragments arranged by size:
4594 3764 1874 1185 785 150
Acc65I G'GTAC_C
Cuts at: 9673 9673
Size: 12352
AclI AA'CG_TT
Cuts at: 1563 10479 10852 1563
Size: 8916 373 3063
Fragments arranged by size:
8916 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2060 2306 2828 3192 3242 3474 4100 5052
Size: 428 246 522 364 50 232 626 952
Cuts at: 5052 5839 7209 8102 8837 8904 9020 9374 9609
Size: 787 1370 893 735 67 116 354 235
Cuts at: 9609 10415 11799 12067 1632
Size: 806 1384 268 1917
Fragments arranged by size:
1917 1384 1370 952 893 806 787 735
626 522 428 364 354 268 246 235
232 116 67 50
AfeI AGC'GCT
Cuts at: 4966 7972 4966
Size: 3006 9346
AflII C'TTAA_G
Cuts at: 820 1017 2455 4591 8408 820
Size: 197 1438 2136 3817 4764
Fragments arranged by size:
4764 3817 2136 1438 197
AflIII A'CryG_T
Cuts at: 1203 6180 11251 12228 1203
Size: 4977 5071 977 1327
Fragments arranged by size:
5071 4977 1327 977
AgeI A'CCGG_T
Cuts at: 11183 11183
Size: 12352
AhdI GACnn_n'nnGTC
Cuts at: 3292 11081 3292
Size: 7789 4563
AleI CACnn'nnGTG
Cuts at: 1166 6512 1166
Size: 5346 7006
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5592 9333 1184
Size: 4408 3741 4203
Fragments arranged by size:
4408 4203 3741
AlwNI CAG_nnn'CTG
Cuts at: 3931 4397 4690 4771 4873 5293 5338 5456 5608
Size: 466 293 81 102 420 45 118 152
Cuts at: 5608 5821 6050 7334 7789 8491 10095 11667 3931
Size: 213 229 1284 455 702 1604 1572 4616
Fragments arranged by size:
4616 1604 1572 1284 702 466 455 420
293 229 213 152 118 102 81 45
ApaI G_GGCC'C
Cuts at: 2769 6880 7673 8458 2769
Size: 4111 793 785 6663
Fragments arranged by size:
6663 4111 793 785
ApaLI G'TGCA_C
Cuts at: 6079 9093 10409 11565 6079
Size: 3014 1316 1156 6866
Fragments arranged by size:
6866 3014 1316 1156
ApoI r'AATT_y
Cuts at: 1493 3406 9661 9887 10039 12148 1493
Size: 1913 6255 226 152 2109 1697
Fragments arranged by size:
6255 2109 1913 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 10906 160
Size: 872 9874 1606
Fragments arranged by size:
9874 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12352
AvaI C'yCGr_G
Cuts at: 1870 1943 2640 4108 5235 5385 5802 5826 6873
Size: 73 697 1468 1127 150 417 24 1047
Cuts at: 6873 6894 6904 7182 8451 9006 9677 1870
Size: 21 10 278 1269 555 671 4545
Fragments arranged by size:
4545 1468 1269 1127 1047 697 671 555
417 278 150 73 24 21 10
BamHI G'GATC_C
Cuts at: 2127 9682 10049 2127
Size: 7555 367 4430
Fragments arranged by size:
7555 4430 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4192 5105 6091 7826 7943
Size: 325 217 627 2405 913 986 1735 117
Cuts at: 7943 9152 9673 11128 618
Size: 1209 521 1455 1842
Fragments arranged by size:
2405 1842 1735 1455 1209 986 913 627
521 325 217 117
BanII G_rGCy'C
Cuts at: 1391 2169 2769 3921 5321 5920 6414 6816 6880
Size: 778 600 1152 1400 599 494 402 64
Cuts at: 6880 7673 7872 8458 8731 8871 8949 9671 1391
Size: 793 199 586 273 140 78 722 4072
Fragments arranged by size:
4072 1400 1152 793 778 722 600 599
586 494 402 273 199 140 78 64
BbeI G_GCGC'C
Cuts at: 7830 7830
Size: 12352
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2072 2321 3056 5946 8090 8547 928
Size: 971 173 249 735 2890 2144 457 4733
Fragments arranged by size:
4733 2890 2144 971 735 457 249 173
BbvCI CC'TCA_GC
Cuts at: 4554 4704 4801 8235 4554
Size: 150 97 3434 8671
Fragments arranged by size:
8671 3434 150 97
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5783 5817 6323 6357 10543 10577 1903
Size: 34 3846 34 506 34 4186 34 3678
Fragments arranged by size:
4186 3846 3678 506 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 10244 11454 10244
Size: 1210 11142
BclI T'GATC_A
Cuts at: 1548 1548
Size: 12352
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3500 3542 3608
Size: 107 122 71 631 605 1828 42 66
Cuts at: 3608 4286 4394 4544 4790 5141 5585 5634 5663
Size: 678 108 150 246 351 444 49 29
Cuts at: 5663 6314 7364 7718 8462 8972 10963 136
Size: 651 1050 354 744 510 1991 1525
Fragments arranged by size:
1991 1828 1525 1050 744 678 651 631
605 510 444 354 351 246 150 122
108 107 71 66 49 42 29
BglII A'GATC_T
Cuts at: 2856 5209 7146 12347 2856
Size: 2353 1937 5201 2861
Fragments arranged by size:
5201 2861 2353 1937
BlpI GC'TnA_GC
Cuts at: 3915 4827 4968 5320 9769 3915
Size: 912 141 352 4449 6498
Fragments arranged by size:
6498 4449 912 352 141
Bme1580I G_kGCm'C
Cuts at: 1790 2374 2769 3003 3901 4153 5108 6083 6691
Size: 584 395 234 898 252 955 975 608
Cuts at: 6691 6880 7673 7885 8458 9097 10413 11569 1790
Size: 189 793 212 573 639 1316 1156 2573
Fragments arranged by size:
2573 1316 1156 975 955 898 793 639
608 584 573 395 252 234 212 189
BmgBI CAC'GTC
Cuts at: 1318 7912 1318
Size: 6594 5758
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5348 5727 6155
Size: 416 53 83 399 24 3931 379 428
Cuts at: 6155 6685 7839 8161 10083 11041 442
Size: 530 1154 322 1922 958 1753
Fragments arranged by size:
3931 1922 1753 1154 958 530 428 416
399 379 322 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2927 1056
Size: 1871 10481
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3373 3670 7117 7921 3373
Size: 297 3447 804 7804
Fragments arranged by size:
7804 3447 804 297
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2228 3302 3408 3508 3836 4592 4793 6173
Size: 989 1074 106 100 328 756 201 1380
Cuts at: 6173 6740 6751 6972 7019 7430 7910 8480 11012
Size: 567 11 221 47 411 480 570 2532
Cuts at: 11012 1239
Size: 2579
Fragments arranged by size:
2579 2532 1380 1074 989 756 570 567
480 411 328 221 201 106 100 47
11
Bpu10I CC'TnA_GC
Cuts at: 4554 4704 4801 5145 7280 8235 4554
Size: 150 97 344 2135 955 8671
Fragments arranged by size:
8671 2135 955 344 150 97
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2921 4058 5999 7089 10477 11342 11640 2921
Size: 1137 1941 1090 3388 865 298 3633
Fragments arranged by size:
3633 3388 1941 1137 1090 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2272 7909 8240 8292 11015 882
Size: 617 773 5637 331 52 2723 2219
Fragments arranged by size:
5637 2723 2219 773 617 331 52
BsaAI yAC'GTr
Cuts at: 493 1204 2917 9649 10143 493
Size: 711 1713 6732 494 2702
Fragments arranged by size:
6732 2702 1713 711 494
BsaBI GATnn'nnATC
Cuts at: 12346 12346
Size: 12352
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2103 4929 5447 7827
Size: 53 83 186 936 570 2826 518 2380
Cuts at: 7827 10159 10541 11176 275
Size: 2332 382 635 1451
Fragments arranged by size:
2826 2380 2332 1451 936 635 570 518
382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5124 10785 11183 11457 11604 12015 5124
Size: 5661 398 274 147 411 5461
Fragments arranged by size:
5661 5461 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2696 2726 2936 2966 1178
Size: 30 165 30 1293 30 210 30 10564
Fragments arranged by size:
10564 1293 210 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2594 3281 3471 3754 4688 5537 6692 7427
Size: 1408 687 190 283 934 849 1155 735
Cuts at: 7427 7676 9062 1186
Size: 249 1386 4476
Fragments arranged by size:
4476 1408 1386 1155 934 849 735 687
283 249 190
BseYI C'CCAG_C
Cuts at: 2140 2153 2213 3096 3435 3495 3944 4092 4397
Size: 13 60 883 339 60 449 148 305
Cuts at: 4397 4478 4866 4947 4961 5331 5762 6084 6229
Size: 81 388 81 14 370 431 322 145
Cuts at: 6229 7197 7201 7484 7666 7789 7931 8135 8212
Size: 968 4 283 182 123 142 204 77
Cuts at: 8212 9330 11555 12051 2140
Size: 1118 2225 496 2441
Fragments arranged by size:
2441 2225 1118 968 883 496 449 431
388 370 339 322 305 283 204 182
148 145 142 123 81 81 77 60
60 14 13 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2693 3560 3768 4784 4901 4943 5163
Size: 573 957 867 208 1016 117 42 220
Cuts at: 5163 5424 5483 5813 6327 6516 7442 7858 8238
Size: 261 59 330 514 189 926 416 380
Cuts at: 8238 8717 1163
Size: 479 4798
Fragments arranged by size:
4798 1016 957 926 867 573 514 479
416 380 330 261 220 208 189 117
59 42
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 9802 10563 11591 1540
Size: 275 186 7801 761 1028 2301
Fragments arranged by size:
7801 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2586 5321 6083 6498 7872 8871 8949 9097 9645
Size: 2735 762 415 1374 999 78 148 548
Cuts at: 9645 10413 10498 11569 2586
Size: 768 85 1071 3369
Fragments arranged by size:
3369 2735 1374 1071 999 768 762 548
415 148 85 78
BsmI GAATG_Cn'
Cuts at: 4747 6771 9863 9956 4747
Size: 2024 3092 93 7143
Fragments arranged by size:
7143 3092 2024 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2582 3111 6128 2582
Size: 529 3017 8806
Fragments arranged by size:
8806 3017 529
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2169 2374 2586 2769 3003 3901 3921
Size: 399 379 205 212 183 234 898 20
Cuts at: 3921 4153 5108 5321 5920 6083 6414 6498 6691
Size: 232 955 213 599 163 331 84 193
Cuts at: 6691 6816 6880 7673 7872 7885 8458 8731 8871
Size: 125 64 793 199 13 573 273 140
Cuts at: 8871 8949 9097 9645 9671 10413 10498 11569 1391
Size: 78 148 548 26 742 85 1071 2174
Fragments arranged by size:
2174 1071 955 898 793 742 599 573
548 399 379 331 273 234 232 213
212 205 199 193 183 163 148 140
125 85 84 78 64 26 20 13
BspEI T'CCGG_A
Cuts at: 5124 12015 5124
Size: 6891 5461
BspHI T'CATG_A
Cuts at: 2358 2392 10241 11971 2358
Size: 34 7849 1730 2739
Fragments arranged by size:
7849 2739 1730 34
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3939 5363 5387 6285 8527 9301 9707 844
Size: 3095 1424 24 898 2242 774 406 3489
Fragments arranged by size:
3489 3095 2242 1424 898 774 406 24
BsrBI CCG'CTC
Cuts at: 1394 1823 2125 7243 7808 10239 1394
Size: 429 302 5118 565 2431 3507
Fragments arranged by size:
5118 3507 2431 565 429 302
BsrDI GCAATG_nn'
Cuts at: 65 1248 2471 3195 4980 5365 7014 7611 7986
Size: 1183 1223 724 1785 385 1649 597 375
Cuts at: 7986 8402 8625 8931 9075 9384 9513 10847 11021
Size: 416 223 306 144 309 129 1334 174
Cuts at: 11021 65
Size: 1396
Fragments arranged by size:
1785 1649 1396 1334 1223 1183 724 597
416 385 375 309 306 223 174 144
129
BsrFI r'CCGG_y
Cuts at: 1700 1810 3200 4859 5742 6923 10996 11183 1700
Size: 110 1390 1659 883 1181 4073 187 2869
Fragments arranged by size:
4073 2869 1659 1390 1181 883 187 110
BsrGI T'GTAC_A
Cuts at: 96 2650 6598 9265 96
Size: 2554 3948 2667 3183
Fragments arranged by size:
3948 3183 2667 2554
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 12352
BssSI C'ACGA_G
Cuts at: 2585 2679 10412 11424 2585
Size: 94 7733 1012 3513
Fragments arranged by size:
7733 3513 1012 94
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2515 3538 4055 4312 4690 5378 6350 7262
Size: 1721 1023 517 257 378 688 972 912
Cuts at: 7262 7334 7478 9257 794
Size: 72 144 1779 3889
Fragments arranged by size:
3889 1779 1721 1023 972 912 688 517
378 257 144 72
BstBI TT'CG_AA
Cuts at: 2007 11156 2007
Size: 9149 3203
BstEII G'GTnAC_C
Cuts at: 1184 7373 7636 7813 8386 1184
Size: 6189 263 177 573 5150
Fragments arranged by size:
6189 5150 573 263 177
BstXI CCAn_nnnn'nTGG
Cuts at: 3010 3965 4045 4087 4261 5101 5788 6514 6616
Size: 955 80 42 174 840 687 726 102
Cuts at: 6616 8119 8293 8383 9097 9172 3010
Size: 1503 174 90 714 75 6190
Fragments arranged by size:
6190 1503 955 840 726 714 687 174
174 102 90 80 75 42
BstYI r'GATC_y
Cuts at: 1972 2127 2289 2754 2856 3648 4605 5209 5452
Size: 155 162 465 102 792 957 604 243
Cuts at: 5452 7146 9594 9682 10049 10434 10451 11903 11989
Size: 1694 2448 88 367 385 17 1452 86
Cuts at: 11989 12001 12347 1972
Size: 12 346 1977
Fragments arranged by size:
2448 1977 1694 1452 957 792 604 465
385 367 346 243 162 155 102 88
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 5660 5814 5833 6792 5660
Size: 154 19 959 11220
Fragments arranged by size:
11220 959 154 19
BtgI C'CryG_G
Cuts at: 513 1065 2022 2091 2548 3787 4260 4467 5991
Size: 552 957 69 457 1239 473 207 1524
Cuts at: 5991 6807 7795 8175 8424 8571 513
Size: 816 988 380 249 147 4294
Fragments arranged by size:
4294 1524 1239 988 957 816 552 473
457 380 249 207 147 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4801 5311 5658 9511 487
Size: 683 56 3575 510 347 3853 3328
Fragments arranged by size:
3853 3575 3328 683 510 347 56
BtsI GCAGTG_nn'
Cuts at: 3744 3838 3882 3901 4902 4913 5466 5601 7131
Size: 94 44 19 1001 11 553 135 1530
Cuts at: 7131 7263 7353 7623 7841 8019 8277 9191 9414
Size: 132 90 270 218 178 258 914 223
Cuts at: 9414 9868 10662 10682 3744
Size: 454 794 20 5414
Fragments arranged by size:
5414 1530 1001 914 794 553 454 270
258 223 218 178 135 132 94 90
44 20 19 11
ClaI AT'CG_AT
Cuts at: 1554 3210 1554
Size: 1656 10696
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2860 2895 6403 6438 8829 8864 8914
Size: 35 2296 35 3508 35 2391 35 50
Cuts at: 8914 8949 529
Size: 35 3932
Fragments arranged by size:
3932 3508 2391 2296 50 35 35 35
35 35
DraI TTT'AAA
Cuts at: 9656 9656
Size: 12352
DraIII CAC_nnn'GTG
Cuts at: 4148 5186 6078 6323 7213 4148
Size: 1038 892 245 890 9287
Fragments arranged by size:
9287 1038 892 890 245
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3781 8114 8927 11359 809
Size: 507 2465 4333 813 2432 1802
Fragments arranged by size:
4333 2465 2432 1802 813 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3454 3589 4408
Size: 54 1425 50 275 18 1624 135 819
Cuts at: 4408 4719 5484 5542 5635 6097 6618 6921 6925
Size: 311 765 58 93 462 521 303 4
Cuts at: 6925 7011 7170 7702 8740 9228 9799 10688 12317
Size: 86 159 532 1038 488 571 889 1629
Cuts at: 12317 8
Size: 43
Fragments arranged by size:
1629 1624 1425 1038 889 819 765 571
532 521 488 462 311 303 275 159
135 93 86 58 54 50 43 18
4
EagI C'GGCC_G
Cuts at: 1537 1812 9799 1537
Size: 275 7987 4090
Fragments arranged by size:
7987 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2421 7746 9459 9564 10282 1353
Size: 1068 5325 1713 105 718 3423
Fragments arranged by size:
5325 3423 1713 1068 718 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 10939 11313 11459 387
Size: 1064 138 9350 374 146 1280
Fragments arranged by size:
9350 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5319 7870 8869 8947 5319
Size: 2551 999 78 8724
Fragments arranged by size:
8724 2551 999 78
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2060 2228 2306 2828 3192 3242 3302
Size: 393 428 168 78 522 364 50 60
Cuts at: 3302 3408 3474 3508 3836 4100 4592 4793 5052
Size: 106 66 34 328 264 492 201 259
Cuts at: 5052 5839 6173 6740 6751 6972 7019 7209 7430
Size: 787 334 567 11 221 47 190 221
Cuts at: 7430 7910 8102 8480 8837 8904 9020 9374 9609
Size: 480 192 378 357 67 116 354 235
Cuts at: 9609 10415 11012 11799 12067 1239
Size: 806 597 787 268 1524
Fragments arranged by size:
1524 806 787 787 597 567 522 492
480 428 393 378 364 357 354 334
328 268 264 259 235 221 221 201
192 190 168 116 106 78 67 66
60 50 47 34 11
EcoNI CCTnn'n_nnAGG
Cuts at: 2822 2966 3848 5519 7049 7412 7466 2822
Size: 144 882 1671 1530 363 54 7708
Fragments arranged by size:
7708 1671 1530 882 363 144 54
EcoO109I rG'GnC_Cy
Cuts at: 2137 2451 2644 2766 3712 3862 4351 5290 5554
Size: 314 193 122 946 150 489 939 264
Cuts at: 5554 5818 5983 6666 6877 6898 7042 7447 7670
Size: 264 165 683 211 21 144 405 223
Cuts at: 7670 8116 8239 8455 8920 9196 2137
Size: 446 123 216 465 276 5293
Fragments arranged by size:
5293 946 939 683 489 465 446 405
314 276 264 264 223 216 211 193
165 150 144 123 122 21
EcoRI G'AATT_C
Cuts at: 9661 10039 9661
Size: 378 11974
EcoRV GAT'ATC
Cuts at: 3214 3214
Size: 12352
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2812 3262 9563 2812
Size: 450 6301 5601
Fragments arranged by size:
6301 5601 450
FseI GG_CCGG'CC
Cuts at: 6927 6927
Size: 12352
FspI TGC'GCA
Cuts at: 3893 4715 4737 10858 3893
Size: 822 22 6121 5387
Fragments arranged by size:
6121 5387 822 22
HaeII r_GCGC'y
Cuts at: 1678 4771 4968 6592 6659 7830 7974 11499 1678
Size: 3093 197 1624 67 1171 144 3525 2531
Fragments arranged by size:
3525 3093 2531 1624 1171 197 144 67
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2673 2705 3254 3278 3286
Size: 32 135 32 1101 32 549 24 8
Cuts at: 3286 3310 3587 3619 3640 3672 5437 5469 5590
Size: 24 277 32 21 32 1765 32 121
Cuts at: 5590 5622 7138 7170 8289 8321 9353 9385 10993
Size: 32 1516 32 1119 32 1032 32 1608
Cuts at: 10993 11025 11067 11099 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1765 1608 1516 1119 1101 1032 549
277 135 121 42 32 32 32 32
32 32 32 32 32 32 32 32
24 24 21 8
HincII GTy'rAC
Cuts at: 669 1657 1938 3309 8829 9696 9942 669
Size: 988 281 1371 5520 867 246 3079
Fragments arranged by size:
5520 3079 1371 988 867 281 246
HindIII A'AGCT_T
Cuts at: 748 2220 3115 5246 748
Size: 1472 895 2131 7854
Fragments arranged by size:
7854 2131 1472 895
HpaI GTT'AAC
Cuts at: 9942 9942
Size: 12352
KasI G'GCGC_C
Cuts at: 7826 7826
Size: 12352
KpnI G_GTAC'C
Cuts at: 9677 9677
Size: 12352
MfeI C'AATT_G
Cuts at: 9951 9951
Size: 12352
MluI A'CGCG_T
Cuts at: 12228 12228
Size: 12352
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2534 3792 5650 5955 6777 8317 11466 11650
Size: 1229 1258 1858 305 822 1540 3149 184
Cuts at: 11650 1305
Size: 2007
Fragments arranged by size:
3149 2007 1858 1540 1258 1229 822 305
184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3591 4721 5544 5637 6620
Size: 54 1425 343 1759 1130 823 93 983
Cuts at: 6620 7172 10
Size: 552 5190
Fragments arranged by size:
5190 1759 1425 1130 983 823 552 343
93 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2351 2357 2523 5948 6074 6512 7124
Size: 648 1185 6 166 3425 126 438 612
Cuts at: 7124 7616 7800 8009 8552 10310 10669 10828 518
Size: 492 184 209 543 1758 359 159 2042
Fragments arranged by size:
3425 2042 1758 1185 648 612 543 492
438 359 209 184 166 159 126 6
MspA1I CmG'CkG
Cuts at: 1606 2144 2656 3453 3677 3944 4061 4096 4340
Size: 538 512 797 224 267 117 35 244
Cuts at: 4340 4655 4687 4703 4733 5030 5080 5540 5572
Size: 315 32 16 30 297 50 460 32
Cuts at: 5572 5616 5625 6025 6035 6341 6546 6997 7168
Size: 44 9 400 10 306 205 451 171
Cuts at: 7168 7201 7687 7733 8135 8327 8998 9263 9768
Size: 33 486 46 402 192 671 265 505
Cuts at: 9768 10445 11593 11838 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 797 677 671 538 512 505
486 460 451 402 400 315 306 297
267 265 245 244 224 205 192 171
117 50 46 44 35 33 32 32
30 16 10 9
NaeI GCC'GGC
Cuts at: 3202 4861 6925 3202
Size: 1659 2064 8629
Fragments arranged by size:
8629 2064 1659
NarI GG'CG_CC
Cuts at: 7827 7827
Size: 12352
NcoI C'CATG_G
Cuts at: 513 1065 2022 2091 2548 4467 6807 7795 8571
Size: 552 957 69 457 1919 2340 988 776
Cuts at: 8571 513
Size: 4294
Fragments arranged by size:
4294 2340 1919 988 957 776 552 457
69
NdeI CA'TA_TG
Cuts at: 387 387
Size: 12352
NgoMIV G'CCGG_C
Cuts at: 3200 4859 6923 3200
Size: 1659 2064 8629
Fragments arranged by size:
8629 2064 1659
NheI G'CTAG_C
Cuts at: 1052 2923 1052
Size: 1871 10481
NotI GC'GGCC_GC
Cuts at: 9799 9799
Size: 12352
NspI r_CATG'y
Cuts at: 1132 1229 2239 2356 3427 3478 3532 4376 4781
Size: 97 1010 117 1071 51 54 844 405
Cuts at: 4781 5157 6184 9710 11255 1132
Size: 376 1027 3526 1545 2229
Fragments arranged by size:
3526 2229 1545 1071 1027 1010 844 405
376 117 97 54 51
PasI CC'CwG_GG
Cuts at: 1348 5353 8293 9193 1348
Size: 4005 2940 900 4507
Fragments arranged by size:
4507 4005 2940 900
PciI A'CATG_T
Cuts at: 6180 11251 6180
Size: 5071 7281
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2632 4171 5293 5338 6287 6463 7915 1108
Size: 1524 1539 1122 45 949 176 1452 5545
Fragments arranged by size:
5545 1539 1524 1452 1122 949 176 45
PfoI T'CCnGG_A
Cuts at: 3066 4014 5865 6141 6150 7305 3066
Size: 948 1851 276 9 1155 8113
Fragments arranged by size:
8113 1851 1155 948 276 9
PmeI GTTT'AAAC
Cuts at: 9656 9656
Size: 12352
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 10421 10453 11934 11966 10421
Size: 32 1481 32 10807
Fragments arranged by size:
10807 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2451 2644 3712 5290 5554 5818 6898 7042 7447
Size: 193 1068 1578 264 264 1080 144 405
Cuts at: 7447 8116 8239 8920 2451
Size: 669 123 681 5883
Fragments arranged by size:
5883 1578 1080 1068 681 669 405 264
264 193 144 123
PshAI GACnn'nnGTC
Cuts at: 1613 2550 1613
Size: 937 11415
PsiI TTA'TAA
Cuts at: 9922 9922
Size: 12352
PspOMI G'GGCC_C
Cuts at: 2765 6876 7669 8454 2765
Size: 4111 793 785 6663
Fragments arranged by size:
6663 4111 793 785
PspXI vC'TCGA_Gb
Cuts at: 5826 5826
Size: 12352
PstI C_TGCA'G
Cuts at: 830 3321 4060 4138 4371 4798 4825 5007 5461
Size: 2491 739 78 233 427 27 182 454
Cuts at: 5461 6034 6505 7069 7138 7258 7344 7732 7836
Size: 573 471 564 69 120 86 388 104
Cuts at: 7836 8326 8799 8962 8980 9457 9704 830
Size: 490 473 163 18 477 247 3478
Fragments arranged by size:
3478 2491 739 573 564 490 477 473
471 454 427 388 247 233 182 163
120 104 86 78 69 27 18
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 12352
PvuII CAG'CTG
Cuts at: 2144 2656 3677 3944 4061 4340 4655 4703 4733
Size: 512 1021 267 117 279 315 48 30
Cuts at: 4733 5030 5080 5540 5625 6025 6035 6341 6546
Size: 297 50 460 85 400 10 306 205
Cuts at: 6546 6997 7201 7687 8135 8327 8998 9263 2144
Size: 451 204 486 448 192 671 265 5233
Fragments arranged by size:
5233 1021 671 512 486 460 451 448
400 315 306 297 279 267 265 205
204 192 117 85 50 48 30 10
RsrII CG'GwC_CG
Cuts at: 6139 6139
Size: 12352
SacI G_AGCT'C
Cuts at: 5321 7872 8871 8949 5321
Size: 2551 999 78 8724
Fragments arranged by size:
8724 2551 999 78
SalI G'TCGA_C
Cuts at: 1936 9694 1936
Size: 7758 4594
SanDI GG'GwC_CC
Cuts at: 2644 3712 5554 6898 2644
Size: 1068 1842 1344 8098
Fragments arranged by size:
8098 1842 1344 1068
SapI GCTCTTCn'nnn_
Cuts at: 9459 9459
Size: 12352
SbfI CC_TGCA'GG
Cuts at: 9704 9704
Size: 12352
ScaI AGT'ACT
Cuts at: 7232 10600 12312 7232
Size: 3368 1712 7272
Fragments arranged by size:
7272 3368 1712
SexAI A'CCwGG_T
Cuts at: 4527 4527
Size: 12352
SfcI C'TryA_G
Cuts at: 826 1045 3317 4056 4134 4293 4367 4794 4821
Size: 219 2272 739 78 159 74 427 27
Cuts at: 4821 5003 5213 5457 6030 6501 7065 7134 7254
Size: 182 210 244 573 471 564 69 120
Cuts at: 7254 7340 7728 7832 8322 8795 8958 8976 9453
Size: 86 388 104 490 473 163 18 477
Cuts at: 9453 9700 10835 11516 11707 826
Size: 247 1135 681 191 1471
Fragments arranged by size:
2272 1471 1135 739 681 573 564 490
477 473 471 427 388 247 244 219
210 191 182 163 159 120 104 86
78 74 69 27 18
SfiI GGCCn_nnn'nGGCC
Cuts at: 7718 7718
Size: 12352
SfoI GGC'GCC
Cuts at: 7828 7828
Size: 12352
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12352
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 12352
SmaI CCC'GGG
Cuts at: 1872 2642 6875 6896 7184 9008 9679 1872
Size: 770 4233 21 288 1824 671 4545
Fragments arranged by size:
4545 4233 1824 770 671 288 21
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2455 2936 4073 4591 5235 5826
Size: 197 926 512 481 1137 518 644 591
Cuts at: 5826 6014 7104 8408 10456 11357 11619 820
Size: 188 1090 1304 2048 901 262 1553
Fragments arranged by size:
2048 1553 1304 1137 1090 926 901 644
591 518 512 481 262 197 188
SnaBI TAC'GTA
Cuts at: 493 9649 10143 493
Size: 9156 494 2702
Fragments arranged by size:
9156 2702 494
SpeI A'CTAG_T
Cuts at: 152 4168 9130 152
Size: 4016 4962 3374
Fragments arranged by size:
4962 4016 3374
SphI G_CATG'C
Cuts at: 1132 3427 3532 4781 9710 1132
Size: 2295 105 1249 4929 3774
Fragments arranged by size:
4929 3774 2295 1249 105
SrfI GCCC'GGGC
Cuts at: 6875 7184 6875
Size: 309 12043
SspI AAT'ATT
Cuts at: 5 52 2402 10276 5
Size: 47 2350 7874 2081
Fragments arranged by size:
7874 2350 2081 47
StuI AGG'CCT
Cuts at: 1517 2726 3611 4853 5039 5798 7112 7952 1517
Size: 1209 885 1242 186 759 1314 840 5917
Fragments arranged by size:
5917 1314 1242 1209 885 840 759 186
StyI C'CwwG_G
Cuts at: 513 1065 2022 2091 2548 2823 2904 3021 3054
Size: 552 957 69 457 275 81 117 33
Cuts at: 3054 4467 4644 4848 5979 6609 6807 7359 7605
Size: 1413 177 204 1131 630 198 552 246
Cuts at: 7605 7795 7818 8112 8376 8559 8571 8583 9018
Size: 190 23 294 264 183 12 12 435
Cuts at: 9018 9139 9791 513
Size: 121 652 3074
Fragments arranged by size:
3074 1413 1131 957 652 630 552 552
457 435 294 275 264 246 204 198
190 183 177 121 117 81 69 33
23 12 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4361 4460 5928 6098 7250 7412 7448
Size: 1255 2781 99 1468 170 1152 162 36
Cuts at: 7448 7484 7532 7541 7961 8204 8712 9140 10379
Size: 36 48 9 420 243 508 428 1239
Cuts at: 10379 10396 10549 10734 11179 325
Size: 17 153 185 445 1498
Fragments arranged by size:
2781 1498 1468 1255 1239 1152 508 445
428 420 243 185 170 162 153 99
48 36 36 17 9
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2262 2650 3160
Size: 274 80 33 51 1443 285 388 510
Cuts at: 3160 4178 5473 6598 7230 8782 9265 9609 10598
Size: 1018 1295 1125 632 1552 483 344 989
Cuts at: 10598 12310 96
Size: 1712 138
Fragments arranged by size:
1712 1552 1443 1295 1125 1018 989 632
510 483 388 344 285 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2696 2728 7197 7199 7229 7231 9136 9168 12312
Size: 32 4469 2 30 2 1905 32 3144
Cuts at: 12312 12344 2696
Size: 32 2704
Fragments arranged by size:
4469 3144 2704 1905 32 32 32 30
2 2
Tth111I GACn'n_nGTC
Cuts at: 1316 4529 1316
Size: 3213 9139
XbaI T'CTAG_A
Cuts at: 5200 9688 5200
Size: 4488 7864
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2759 4525 4627 5494 8761 9094 2759
Size: 1766 102 867 3267 333 6017
Fragments arranged by size:
6017 3267 1766 867 333 102
XhoI C'TCGA_G
Cuts at: 1943 5235 5826 1943
Size: 3292 591 8469
Fragments arranged by size:
8469 3292 591
XmaI C'CCGG_G
Cuts at: 1870 2640 6873 6894 7182 9006 9677 1870
Size: 770 4233 21 288 1824 671 4545
Fragments arranged by size:
4545 4233 1824 770 671 288 21
XmnI GAAnn'nnTTC
Cuts at: 2813 9546 10481 2813
Size: 6733 935 4684
Fragments arranged by size:
6733 4684 935
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5448 10160 276
Size: 53 83 186 936 3914 4712 2468
Fragments arranged by size:
4712 3914 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I FspAI
NruI NsiI PacI PmlI PsrI SacII SwaI