Restriction map for
pFN21ASDA1024
(Circular) (Six-base) MAPSORT of: pFN21ASDA1024.seq Check: 7542 from: 1 to: 7477
pFN21ASDA1024 7477 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4384 4384
Size: 7477
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5287 278
Size: 53 83 186 936 3751 2468
Fragments arranged by size:
3751 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4820 1937
Size: 2883 4594
Acc65I G'GTAC_C
Cuts at: 4798 4798
Size: 7477
AclI AA'CG_TT
Cuts at: 1563 5604 5977 1563
Size: 4041 373 3063
Fragments arranged by size:
4041 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3818 5540 6924 7192 1632
Size: 2186 1722 1384 268 1917
Fragments arranged by size:
2186 1917 1722 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7280
AflIII A'CryG_T
Cuts at: 1203 6376 7353 1203
Size: 5173 977 1327
Fragments arranged by size:
5173 1327 977
AgeI A'CCGG_T
Cuts at: 6308 6308
Size: 7477
AhdI GACnn_n'nnGTC
Cuts at: 6206 6206
Size: 7477
AleI CACnn'nnGTG
Cuts at: 1166 3053 3626 1166
Size: 1887 573 5017
Fragments arranged by size:
5017 1887 573
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3836 1184
Size: 2652 4825
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4382 4414 4382
Size: 32 7445
AlwNI CAG_nnn'CTG
Cuts at: 2110 2614 2959 3081 4218 5220 6792 2110
Size: 504 345 122 1137 1002 1572 2795
Fragments arranged by size:
2795 1572 1137 1002 504 345 122
ApaI G_GGCC'C
Cuts at: 3048 3048
Size: 7477
ApaLI G'TGCA_C
Cuts at: 3493 5534 6690 3493
Size: 2041 1156 4280
Fragments arranged by size:
4280 2041 1156
ApoI r'AATT_y
Cuts at: 1493 4670 4786 5012 5164 7273 1493
Size: 3177 116 226 152 2109 1697
Fragments arranged by size:
3177 2109 1697 226 152 116
AseI AT'TA_AT
Cuts at: 160 1032 6031 160
Size: 872 4999 1606
Fragments arranged by size:
4999 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7477
AvaI C'yCGr_G
Cuts at: 1870 1943 4802 1870
Size: 73 2859 4545
Fragments arranged by size:
4545 2859 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3623 3656 3623
Size: 33 7444
BamHI G'GATC_C
Cuts at: 3659 4807 5174 3659
Size: 1148 367 5962
Fragments arranged by size:
5962 1148 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3252 3446 4798 6253 618
Size: 325 217 627 1465 194 1352 1455 1842
Fragments arranged by size:
1842 1465 1455 1352 627 325 217 194
BanII G_rGCy'C
Cuts at: 1391 2430 3048 3327 3474 4796 1391
Size: 1039 618 279 147 1322 4072
Fragments arranged by size:
4072 1322 1039 618 279 147
BbeI G_GCGC'C
Cuts at: 3256 3256
Size: 7477
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3806 4626 928
Size: 971 1907 820 3779
Fragments arranged by size:
3779 1907 971 820
BbvCI CC'TCA_GC
Cuts at: 2640 2640
Size: 7477
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5668 5702 1903
Size: 34 3731 34 3678
Fragments arranged by size:
3731 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4631 5369 6579 4631
Size: 738 1210 5529
Fragments arranged by size:
5529 1210 738
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7477
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6088 136
Size: 107 122 71 631 605 4416 1525
Fragments arranged by size:
4416 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2061 2403 3339 3955 7472 2061
Size: 342 936 616 3517 2066
Fragments arranged by size:
3517 2066 936 616 342
BlpI GC'TnA_GC
Cuts at: 3422 4894 3422
Size: 1472 6005
Bme1580I G_kGCm'C
Cuts at: 1790 2131 3048 3449 3497 4752 5538 6694 1790
Size: 341 917 401 48 1255 786 1156 2573
Fragments arranged by size:
2573 1255 1156 917 786 401 341 48
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7477
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2832 5208 6166
Size: 416 53 83 399 24 1415 2376 958
Cuts at: 6166 442
Size: 1753
Fragments arranged by size:
2376 1753 1415 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 4083 1056
Size: 3027 4450
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3148 3380 3488 4617 6137 1239
Size: 1909 232 108 1129 1520 2579
Fragments arranged by size:
2579 1909 1520 1129 232 108
Bpu10I CC'TnA_GC
Cuts at: 2640 3702 3824 2640
Size: 1062 122 6293
Fragments arranged by size:
6293 1062 122
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4412 5602 6467 6765 4412
Size: 1190 865 298 5124
Fragments arranged by size:
5124 1190 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2021 2732 3480 6140 882
Size: 617 522 711 748 2660 2219
Fragments arranged by size:
2660 2219 748 711 617 522
BsaAI yAC'GTr
Cuts at: 493 1204 3463 4774 5268 493
Size: 711 2259 1311 494 2702
Fragments arranged by size:
2702 2259 1311 711 494
BsaBI GATnn'nnATC
Cuts at: 7471 7471
Size: 7477
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3253 5284 5666 6301
Size: 53 83 186 936 1720 2031 382 635
Cuts at: 6301 275
Size: 1451
Fragments arranged by size:
2031 1720 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5910 6308 6582 6729 7140 5910
Size: 398 274 147 411 6247
Fragments arranged by size:
6247 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2693 2723 4013 4043 4078
Size: 30 165 30 1290 30 1290 30 35
Cuts at: 4078 4108 4382 4406 4412 4436 1178
Size: 30 274 24 6 24 4219
Fragments arranged by size:
4219 1290 1290 274 165 35 30 30
30 30 30 24 24 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2771 3206 4178 4274 4277 4535 1186
Size: 1585 435 972 96 3 258 4128
Fragments arranged by size:
4128 1585 972 435 258 96 3
BseYI C'CCAG_C
Cuts at: 3238 6680 7176 3238
Size: 3442 496 3539
Fragments arranged by size:
3539 3442 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3391 3968 4741 1163
Size: 573 1655 577 773 3899
Fragments arranged by size:
3899 1655 773 577 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4927 5688 6716 1540
Size: 275 186 2926 761 1028 2301
Fragments arranged by size:
2926 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2172 2877 3462 3497 3708 3825 5538 5623 6694
Size: 705 585 35 211 117 1713 85 1071
Cuts at: 6694 2172
Size: 2955
Fragments arranged by size:
2955 1713 1071 705 585 211 117 85
35
BsmI GAATG_Cn'
Cuts at: 4540 4988 5081 4540
Size: 448 93 6936
Fragments arranged by size:
6936 448 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2131 2172 2430 2877 3048 3327 3449
Size: 399 341 41 258 447 171 279 122
Cuts at: 3449 3462 3474 3497 3708 3825 4752 4796 5538
Size: 13 12 23 211 117 927 44 742
Cuts at: 5538 5623 6694 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 927 742 447 399 341 279
258 211 171 122 117 85 44 41
23 13 12
BspEI T'CCGG_A
Cuts at: 7140 7140
Size: 7477
BspHI T'CATG_A
Cuts at: 5366 7096 5366
Size: 1730 5747
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4384 4578 4832 844
Size: 3540 194 254 3489
Fragments arranged by size:
3540 3489 254 194
BsrBI CCG'CTC
Cuts at: 1394 1823 5364 1394
Size: 429 3541 3507
Fragments arranged by size:
3541 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2416 3297 5972 6146 65
Size: 1183 1168 881 2675 174 1396
Fragments arranged by size:
2675 1396 1183 1168 881 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3762 6121 6308 1700
Size: 110 1952 2359 187 2869
Fragments arranged by size:
2869 2359 1952 187 110
BsrGI T'GTAC_A
Cuts at: 96 4455 96
Size: 4359 3118
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7477
BssSI C'ACGA_G
Cuts at: 5537 6549 5537
Size: 1012 6465
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2517 3405 4357 4535 794
Size: 1723 888 952 178 3736
Fragments arranged by size:
3736 1723 952 888 178
BstBI TT'CG_AA
Cuts at: 2007 6281 2007
Size: 4274 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7477
BstXI CCAn_nnnn'nTGG
Cuts at: 2312 3442 4246 2312
Size: 1130 804 5543
Fragments arranged by size:
5543 1130 804
BstYI r'GATC_y
Cuts at: 1972 2061 2403 3339 3659 3955 4807 5174 5559
Size: 89 342 936 320 296 852 367 385
Cuts at: 5559 5576 7028 7114 7126 7472 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 936 852 385 367 346 342
320 296 89 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2340 3621 4033 513
Size: 552 957 318 1281 412 3957
Fragments arranged by size:
3957 1281 957 552 412 318
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6738
Fragments arranged by size:
6738 683 56
BtsI GCAGTG_nn'
Cuts at: 2835 4993 5787 5807 2835
Size: 2158 794 20 4505
Fragments arranged by size:
4505 2158 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7477
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7442
DraI TTT'AAA
Cuts at: 3388 4590 4781 3388
Size: 1202 191 6084
Fragments arranged by size:
6084 1202 191
DraIII CAC_nnn'GTG
Cuts at: 4025 4025
Size: 7477
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6484 809
Size: 507 5168 1802
Fragments arranged by size:
5168 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3113 4482 4924
Size: 54 1425 50 275 18 1283 1369 442
Cuts at: 4924 5813 7442 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1369 1283 889 442 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4924 1537
Size: 275 3112 4090
Fragments arranged by size:
4090 3112 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2054 2177 3786 4136 4436 5407 1353
Size: 701 123 1609 350 300 971 3423
Fragments arranged by size:
3423 1609 971 701 350 300 123
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6064 6438 6584 387
Size: 1064 138 4475 374 146 1280
Fragments arranged by size:
4475 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3148 3380 3488 3818 4617 5540 6137
Size: 393 1516 232 108 330 799 923 597
Cuts at: 6137 6924 7192 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1516 923 799 787 597 393 330
268 232 108
EcoNI CCTnn'n_nnAGG
Cuts at: 4160 4256 4160
Size: 96 7381
EcoO109I rG'GnC_Cy
Cuts at: 2107 2611 2661 3733 4152 2107
Size: 504 50 1072 419 5432
Fragments arranged by size:
5432 1072 504 419 50
EcoRI G'AATT_C
Cuts at: 4670 4786 5164 4670
Size: 116 378 6983
Fragments arranged by size:
6983 378 116
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2758 2932 2758
Size: 174 7303
FspI TGC'GCA
Cuts at: 5983 5983
Size: 7477
HaeII r_GCGC'y
Cuts at: 1678 3082 3256 6624 1678
Size: 1404 174 3368 2531
Fragments arranged by size:
3368 2531 1404 174
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2623 2655 2693 2725 3329
Size: 32 135 32 1051 32 38 32 604
Cuts at: 3329 3361 3536 3568 3866 3898 3947 3968 3979
Size: 32 175 32 298 32 49 21 11
Cuts at: 3979 3983 4000 4015 4671 4687 4703 4719 6118
Size: 4 17 15 656 16 16 16 1399
Cuts at: 6118 6150 6192 6224 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1399 1051 656 604 298 175 135
49 42 38 32 32 32 32 32
32 32 32 32 21 17 16 16
16 15 11 4
HincII GTy'rAC
Cuts at: 669 1657 1938 3355 4821 5067 669
Size: 988 281 1417 1466 246 3079
Fragments arranged by size:
3079 1466 1417 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3010 748
Size: 2262 5215
HpaI GTT'AAC
Cuts at: 5067 5067
Size: 7477
KasI G'GCGC_C
Cuts at: 3252 3252
Size: 7477
KpnI G_GTAC'C
Cuts at: 4802 4802
Size: 7477
MfeI C'AATT_G
Cuts at: 5076 5076
Size: 7477
MluI A'CGCG_T
Cuts at: 7353 7353
Size: 7477
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2373 4227 6591 6775 1305
Size: 1068 1854 2364 184 2007
Fragments arranged by size:
2364 2007 1854 1068 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3115 10
Size: 54 1425 343 1283 4372
Fragments arranged by size:
4372 1425 1283 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3053 3626 4463 5435 5794 5953 518
Size: 648 1887 573 837 972 359 159 2042
Fragments arranged by size:
2042 1887 972 837 648 573 359 159
MspA1I CmG'CkG
Cuts at: 1606 2417 4893 5570 6718 6963 1606
Size: 811 2476 677 1148 245 2120
Fragments arranged by size:
2476 2120 1148 811 677 245
NarI GG'CG_CC
Cuts at: 3253 3253
Size: 7477
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5968
Fragments arranged by size:
5968 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7477
NheI G'CTAG_C
Cuts at: 1052 4079 1052
Size: 3027 4450
NotI GC'GGCC_GC
Cuts at: 4924 4924
Size: 7477
NspI r_CATG'y
Cuts at: 1132 1229 4468 4725 4835 6380 1132
Size: 97 3239 257 110 1545 2229
Fragments arranged by size:
3239 2229 1545 257 110 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7477
PciI A'CATG_T
Cuts at: 6376 6376
Size: 7477
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3126 3892 4218 1108
Size: 2018 766 326 4367
Fragments arranged by size:
4367 2018 766 326
PfoI T'CCnGG_A
Cuts at: 4633 4633
Size: 7477
PmeI GTTT'AAAC
Cuts at: 4781 4781
Size: 7477
PmlI CAC'GTG
Cuts at: 3463 3463
Size: 7477
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4382 4414 5546 5578 7059 7091 4382
Size: 32 1132 32 1481 32 4768
Fragments arranged by size:
4768 1481 1132 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2107 2661 3733 4152 2107
Size: 554 1072 419 5432
Fragments arranged by size:
5432 1072 554 419
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7477
PsiI TTA'TAA
Cuts at: 5047 5047
Size: 7477
PspOMI G'GGCC_C
Cuts at: 3044 3044
Size: 7477
PstI C_TGCA'G
Cuts at: 830 4575 4829 830
Size: 3745 254 3478
Fragments arranged by size:
3745 3478 254
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7477
PvuII CAG'CTG
Cuts at: 2417 2417
Size: 7477
SalI G'TCGA_C
Cuts at: 1936 4819 1936
Size: 2883 4594
SanDI GG'GwC_CC
Cuts at: 3733 3733
Size: 7477
SapI GCTCTTCn'nnn_
Cuts at: 2177 3786 4436 2177
Size: 1609 650 5218
Fragments arranged by size:
5218 1609 650
SbfI CC_TGCA'GG
Cuts at: 4829 4829
Size: 7477
ScaI AGT'ACT
Cuts at: 4610 5725 7437 4610
Size: 1115 1712 4650
Fragments arranged by size:
4650 1712 1115
SfcI C'TryA_G
Cuts at: 826 1045 4571 4825 5960 6641 6832 826
Size: 219 3526 254 1135 681 191 1471
Fragments arranged by size:
3526 1471 1135 681 254 219 191
SfoI GGC'GCC
Cuts at: 3254 3254
Size: 7477
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7477
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7477
SmaI CCC'GGG
Cuts at: 1872 4804 1872
Size: 2932 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4427 5581 6482 6744 820
Size: 197 926 2484 1154 901 262 1553
Fragments arranged by size:
2484 1553 1154 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4774 5268 493
Size: 4281 494 2702
Fragments arranged by size:
4281 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7477
SphI G_CATG'C
Cuts at: 1132 4725 4835 1132
Size: 3593 110 3774
Fragments arranged by size:
3774 3593 110
SspI AAT'ATT
Cuts at: 5 52 5401 5
Size: 47 5349 2081
Fragments arranged by size:
5349 2081 47
StuI AGG'CCT
Cuts at: 1517 4115 1517
Size: 2598 4879
StyI C'CwwG_G
Cuts at: 513 1065 2022 2325 4683 4916 513
Size: 552 957 303 2358 233 3074
Fragments arranged by size:
3074 2358 957 552 303 233
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3464 3791 4490 5504 5521 5674 5859
Size: 1255 1884 327 699 1014 17 153 185
Cuts at: 5859 6304 325
Size: 445 1498
Fragments arranged by size:
1884 1498 1255 1014 699 445 327 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3513 4455 4608
Size: 274 80 33 51 1443 1536 942 153
Cuts at: 4608 5723 7435 96
Size: 1115 1712 138
Fragments arranged by size:
1712 1536 1443 1115 942 274 153 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2514 2546 4013 4045 4078 4110 7437 7469 2514
Size: 32 1467 32 33 32 3327 32 2522
Fragments arranged by size:
3327 2522 1467 33 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7477
XbaI T'CTAG_A
Cuts at: 4813 4813
Size: 7477
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3055 3123 4022 3055
Size: 68 899 6510
Fragments arranged by size:
6510 899 68
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7477
XmaI C'CCGG_G
Cuts at: 1870 4802 1870
Size: 2932 4545
XmnI GAAnn'nnTTC
Cuts at: 2798 5606 2798
Size: 2808 4669
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5285 276
Size: 53 83 186 936 3751 2468
Fragments arranged by size:
3751 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AscI AvrII BfrBI BplI BsiWI BsmBI BstZ17I
Bsu36I EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI
NsiI PacI PspXI PsrI RsrII SacI SacII SexAI
SfiI SrfI SwaI