Restriction map for
pFN21ASDA1020
(Circular) (Six-base) MAPSORT of: pFN21ASDA1020.seq Check: 9450 from: 1 to: 6574
pFN21ASDA1020 6574 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3852 3852
Size: 6574
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2166 4384 278
Size: 53 83 186 936 630 2218 2468
Fragments arranged by size:
2468 2218 936 630 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3917 1937
Size: 1980 4594
Acc65I G'GTAC_C
Cuts at: 3895 3895
Size: 6574
AclI AA'CG_TT
Cuts at: 1563 4701 5074 1563
Size: 3138 373 3063
Fragments arranged by size:
3138 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2353 3716 4637 6021 6289 1632
Size: 721 1363 921 1384 268 1917
Fragments arranged by size:
1917 1384 1363 921 721 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6377
AflIII A'CryG_T
Cuts at: 1203 3297 3668 5473 6450 1203
Size: 2094 371 1805 977 1327
Fragments arranged by size:
2094 1805 1327 977 371
AgeI A'CCGG_T
Cuts at: 5405 5405
Size: 6574
AhdI GACnn_n'nnGTC
Cuts at: 5303 5303
Size: 6574
AleI CACnn'nnGTG
Cuts at: 1166 2047 3762 1166
Size: 881 1715 3978
Fragments arranged by size:
3978 1715 881
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2354 2440 3411 1184
Size: 1170 86 971 4347
Fragments arranged by size:
4347 1170 971 86
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3413 3445 3413
Size: 32 6542
AlwNI CAG_nnn'CTG
Cuts at: 2119 2515 2755 4317 5889 2119
Size: 396 240 1562 1572 2804
Fragments arranged by size:
2804 1572 1562 396 240
ApaI G_GGCC'C
Cuts at: 2037 2085 2200 2578 3093 3183 2037
Size: 48 115 378 515 90 5428
Fragments arranged by size:
5428 515 378 115 90 48
ApaLI G'TGCA_C
Cuts at: 3767 4631 5787 3767
Size: 864 1156 4554
Fragments arranged by size:
4554 1156 864
ApoI r'AATT_y
Cuts at: 1493 3883 4109 4261 6370 1493
Size: 2390 226 152 2109 1697
Fragments arranged by size:
2390 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5128 160
Size: 872 4096 1606
Fragments arranged by size:
4096 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6574
AvaI C'yCGr_G
Cuts at: 1870 1943 2408 3106 3772 3899 1870
Size: 73 465 698 666 127 4545
Fragments arranged by size:
4545 698 666 465 127 73
BamHI G'GATC_C
Cuts at: 3904 4271 3904
Size: 367 6207
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2921 2983 3470 3659 3895
Size: 325 217 627 1134 62 487 189 236
Cuts at: 3895 5350 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1134 627 487 325 236 217
189 62
BanII G_rGCy'C
Cuts at: 1391 2037 2085 2200 2364 2556 2578 2595 2937
Size: 646 48 115 164 192 22 17 342
Cuts at: 2937 3093 3183 3195 3528 3893 1391
Size: 156 90 12 333 365 4072
Fragments arranged by size:
4072 646 365 342 333 192 164 156
115 90 48 22 17 12
BbeI G_GCGC'C
Cuts at: 2987 2987
Size: 6574
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3249 3563 3602 928
Size: 971 1350 314 39 3900
Fragments arranged by size:
3900 1350 971 314 39
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3005 3039 4765 4799 1903
Size: 34 1068 34 1726 34 3678
Fragments arranged by size:
3678 1726 1068 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4466 5676 4466
Size: 1210 5364
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6574
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2685 2856 2992
Size: 107 122 71 631 605 1013 171 136
Cuts at: 2992 3518 3690 5185 136
Size: 526 172 1495 1525
Fragments arranged by size:
1525 1495 1013 631 605 526 172 171
136 122 107 71
BglII A'GATC_T
Cuts at: 2307 6569 2307
Size: 4262 2312
BlpI GC'TnA_GC
Cuts at: 2840 3522 3991 2840
Size: 682 469 5423
Fragments arranged by size:
5423 682 469
Bme1580I G_kGCm'C
Cuts at: 1790 2037 2085 2200 2578 2924 3093 3183 3771
Size: 247 48 115 378 346 169 90 588
Cuts at: 3771 4635 5791 1790
Size: 864 1156 2573
Fragments arranged by size:
2573 1156 864 588 378 346 247 169
115 90 48
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6574
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3498 4305 5263
Size: 416 53 83 399 24 2081 807 958
Cuts at: 5263 442
Size: 1753
Fragments arranged by size:
2081 1753 958 807 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6574
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2384 2966 2999 5234 1239
Size: 1145 582 33 2235 2579
Fragments arranged by size:
2579 2235 1145 582 33
Bpu10I CC'TnA_GC
Cuts at: 2969 2969
Size: 6574
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2423 4699 5564 5862 2423
Size: 2276 865 298 3135
Fragments arranged by size:
3135 2276 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5237 882
Size: 617 3738 2219
Fragments arranged by size:
3738 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3403 3871 4365 493
Size: 711 2199 468 494 2702
Fragments arranged by size:
2702 2199 711 494 468
BsaBI GATnn'nnATC
Cuts at: 6568 6568
Size: 6574
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2163 2984 4381 4763
Size: 53 83 186 936 630 821 1397 382
Cuts at: 4763 5398 275
Size: 635 1451
Fragments arranged by size:
1451 1397 936 821 635 630 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2046 5007 5405 5679 5826 6237 2046
Size: 2961 398 274 147 411 2383
Fragments arranged by size:
2961 2383 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6349
Fragments arranged by size:
6349 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2369 3128 3227 3230 3233 3491 3494 1186
Size: 1183 759 99 3 3 258 3 4266
Fragments arranged by size:
4266 1183 759 258 99 3 3 3
BseYI C'CCAG_C
Cuts at: 2099 2342 2680 2755 2835 2914 3377 3571 3819
Size: 243 338 75 80 79 463 194 248
Cuts at: 3819 5777 6273 2099
Size: 1958 496 2400
Fragments arranged by size:
2400 1958 496 463 338 248 243 194
80 79 75
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2234 1163
Size: 573 498 5503
Fragments arranged by size:
5503 573 498
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4024 4785 5813 1540
Size: 275 186 2023 761 1028 2301
Fragments arranged by size:
2301 2023 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2595 3759 3771 4635 4720 5791 2595
Size: 1164 12 864 85 1071 3378
Fragments arranged by size:
3378 1164 1071 864 85 12
BsmI GAATG_Cn'
Cuts at: 3354 4085 4178 3354
Size: 731 93 5750
Fragments arranged by size:
5750 731 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3314 3314
Size: 6574
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2037 2085 2200 2364 2556 2578 2595
Size: 399 247 48 115 164 192 22 17
Cuts at: 2595 2924 2937 3093 3183 3195 3528 3759 3771
Size: 329 13 156 90 12 333 231 12
Cuts at: 3771 3893 4635 4720 5791 1391
Size: 122 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 333 329 247 231
192 164 156 122 115 90 85 48
22 17 13 12 12
BspEI T'CCGG_A
Cuts at: 6237 6237
Size: 6574
BspHI T'CATG_A
Cuts at: 4463 6193 4463
Size: 1730 4844
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3852 3929 844
Size: 3008 77 3489
Fragments arranged by size:
3489 3008 77
BsrBI CCG'CTC
Cuts at: 1394 1823 3076 3258 4461 1394
Size: 429 1253 182 1203 3507
Fragments arranged by size:
3507 1253 1203 429 182
BsrDI GCAATG_nn'
Cuts at: 65 1248 5069 5243 65
Size: 1183 3821 174 1396
Fragments arranged by size:
3821 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2447 3303 5218 5405 1700
Size: 110 637 856 1915 187 2869
Fragments arranged by size:
2869 1915 856 637 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6574
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6574
BssSI C'ACGA_G
Cuts at: 2555 3590 4634 5646 2555
Size: 1035 1044 1012 3483
Fragments arranged by size:
3483 1044 1035 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2119 3360 3506 794
Size: 1325 1241 146 3862
Fragments arranged by size:
3862 1325 1241 146
BstBI TT'CG_AA
Cuts at: 2007 5378 2007
Size: 3371 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6574
BstXI CCAn_nnnn'nTGG
Cuts at: 2488 2944 2488
Size: 456 6118
BstYI r'GATC_y
Cuts at: 1972 2307 2941 3904 4271 4656 4673 6125 6211
Size: 335 634 963 367 385 17 1452 86
Cuts at: 6211 6223 6569 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 963 634 385 367 346 335
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2520 2937 513
Size: 552 957 498 417 4150
Fragments arranged by size:
4150 957 552 498 417
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3726 487
Size: 683 56 2500 3335
Fragments arranged by size:
3335 2500 683 56
BtsI GCAGTG_nn'
Cuts at: 2382 3469 4090 4884 4904 2382
Size: 1087 621 794 20 4052
Fragments arranged by size:
4052 1087 794 621 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6574
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3616 3651 529
Size: 35 3052 35 3452
Fragments arranged by size:
3452 3052 35 35
DraI TTT'AAA
Cuts at: 3878 3878
Size: 6574
DraIII CAC_nnn'GTG
Cuts at: 3506 3506
Size: 6574
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5581 809
Size: 507 4265 1802
Fragments arranged by size:
4265 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3277 4021 4910
Size: 54 1425 50 275 18 1447 744 889
Cuts at: 4910 6539 8
Size: 1629 43
Fragments arranged by size:
1629 1447 1425 889 744 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4021 1537
Size: 275 2209 4090
Fragments arranged by size:
4090 2209 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2999 3417 3435 4504 1353
Size: 1646 418 18 1069 3423
Fragments arranged by size:
3423 1646 1069 418 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5161 5535 5681 387
Size: 1064 138 3572 374 146 1280
Fragments arranged by size:
3572 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2593 2593
Size: 6574
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2353 2384 2966 2999 3716 4637 5234
Size: 393 721 31 582 33 717 921 597
Cuts at: 5234 6021 6289 1239
Size: 787 268 1524
Fragments arranged by size:
1524 921 787 721 717 597 582 393
268 33 31
EcoNI CCTnn'n_nnAGG
Cuts at: 2978 2978
Size: 6574
EcoO109I rG'GnC_Cy
Cuts at: 2034 2081 2574 2807 3089 3098 3180 3447 3490
Size: 47 493 233 282 9 82 267 43
Cuts at: 3490 3568 2034
Size: 78 5040
Fragments arranged by size:
5040 493 282 267 233 82 78 47
43 9
EcoRI G'AATT_C
Cuts at: 3883 4261 3883
Size: 378 6196
FspI TGC'GCA
Cuts at: 3252 3399 3848 5080 3252
Size: 147 449 1232 4746
Fragments arranged by size:
4746 1232 449 147
FspAI rTGC'GCAy
Cuts at: 3399 3399
Size: 6574
HaeII r_GCGC'y
Cuts at: 1678 2987 3291 5721 1678
Size: 1309 304 2430 2531
Fragments arranged by size:
2531 2430 1309 304
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3311 3343 3479 3511 5215
Size: 32 135 32 1739 32 136 32 1704
Cuts at: 5215 5247 5289 5321 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1739 1704 136 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3918 4164 669
Size: 988 281 1980 246 3079
Fragments arranged by size:
3079 1980 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6574
HpaI GTT'AAC
Cuts at: 4164 4164
Size: 6574
KasI G'GCGC_C
Cuts at: 2983 2983
Size: 6574
KpnI G_GTAC'C
Cuts at: 3899 3899
Size: 6574
MfeI C'AATT_G
Cuts at: 4173 4173
Size: 6574
MluI A'CGCG_T
Cuts at: 3668 6450 3668
Size: 2782 3792
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5688 5872 1305
Size: 4383 184 2007
Fragments arranged by size:
4383 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4752
Fragments arranged by size:
4752 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2047 2171 2174 2279 2486 3762 3764
Size: 648 881 124 3 105 207 1276 2
Cuts at: 3764 4532 4891 5050 518
Size: 768 359 159 2042
Fragments arranged by size:
2042 1276 881 768 648 359 207 159
124 105 3 2
MspA1I CmG'CkG
Cuts at: 1606 2128 2404 2512 2684 2736 2839 3276 3799
Size: 522 276 108 172 52 103 437 523
Cuts at: 3799 3990 4667 5815 6060 1606
Size: 191 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 523 522 437 276 245
191 172 108 103 52
NaeI GCC'GGC
Cuts at: 2449 2449
Size: 6574
NarI GG'CG_CC
Cuts at: 2984 2984
Size: 6574
NcoI C'CATG_G
Cuts at: 513 1065 2022 2937 513
Size: 552 957 915 4150
Fragments arranged by size:
4150 957 915 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6574
NgoMIV G'CCGG_C
Cuts at: 2447 2447
Size: 6574
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6574
NotI GC'GGCC_GC
Cuts at: 4021 4021
Size: 6574
NspI r_CATG'y
Cuts at: 1132 1229 3301 3679 3727 3763 3853 3932 5477
Size: 97 2072 378 48 36 90 79 1545
Cuts at: 5477 1132
Size: 2229
Fragments arranged by size:
2229 2072 1545 378 97 90 79 48
36
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6574
PciI A'CATG_T
Cuts at: 3297 5473 3297
Size: 2176 4398
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6574
PmeI GTTT'AAAC
Cuts at: 3878 3878
Size: 6574
PmlI CAC'GTG
Cuts at: 3403 3403
Size: 6574
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4643 4675 6156 6188 4643
Size: 32 1481 32 5029
Fragments arranged by size:
5029 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2807 3098 3490 3568 2807
Size: 291 392 78 5813
Fragments arranged by size:
5813 392 291 78
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6574
PsiI TTA'TAA
Cuts at: 4144 4144
Size: 6574
PspOMI G'GGCC_C
Cuts at: 2033 2081 2196 2574 3089 3179 2033
Size: 48 115 378 515 90 5428
Fragments arranged by size:
5428 515 378 115 90 48
PstI C_TGCA'G
Cuts at: 830 2127 2714 3926 830
Size: 1297 587 1212 3478
Fragments arranged by size:
3478 1297 1212 587
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6574
PvuII CAG'CTG
Cuts at: 2128 2404 2684 2839 3799 2128
Size: 276 280 155 960 4903
Fragments arranged by size:
4903 960 280 276 155
SacI G_AGCT'C
Cuts at: 2595 2595
Size: 6574
SalI G'TCGA_C
Cuts at: 1936 3916 1936
Size: 1980 4594
SanDI GG'GwC_CC
Cuts at: 3098 3098
Size: 6574
SapI GCTCTTCn'nnn_
Cuts at: 2999 3417 2999
Size: 418 6156
SbfI CC_TGCA'GG
Cuts at: 3926 3926
Size: 6574
ScaI AGT'ACT
Cuts at: 4822 6534 4822
Size: 1712 4862
SfcI C'TryA_G
Cuts at: 826 1045 2123 2710 3922 5057 5738 5929 826
Size: 219 1078 587 1212 1135 681 191 1471
Fragments arranged by size:
1471 1212 1135 1078 681 587 219 191
SfoI GGC'GCC
Cuts at: 2985 2985
Size: 6574
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6574
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6574
SmaI CCC'GGG
Cuts at: 1872 3108 3901 1872
Size: 1236 793 4545
Fragments arranged by size:
4545 1236 793
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2438 4678 5579 5841 820
Size: 197 926 495 2240 901 262 1553
Fragments arranged by size:
2240 1553 926 901 495 262 197
SnaBI TAC'GTA
Cuts at: 493 3871 4365 493
Size: 3378 494 2702
Fragments arranged by size:
3378 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6574
SphI G_CATG'C
Cuts at: 1132 3727 3853 3932 1132
Size: 2595 126 79 3774
Fragments arranged by size:
3774 2595 126 79
SspI AAT'ATT
Cuts at: 5 52 4498 5
Size: 47 4446 2081
Fragments arranged by size:
4446 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6574
StyI C'CwwG_G
Cuts at: 513 1065 2022 2037 2145 2937 3093 3366 4013
Size: 552 957 15 108 792 156 273 647
Cuts at: 4013 513
Size: 3074
Fragments arranged by size:
3074 957 792 647 552 273 156 108
15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3517 3535 3833 4601 4618 4771 4956
Size: 1255 1937 18 298 768 17 153 185
Cuts at: 4956 5401 325
Size: 445 1498
Fragments arranged by size:
1937 1498 1255 768 445 298 185 153
18 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4820 6532 96
Size: 274 80 33 51 1443 2843 1712 138
Fragments arranged by size:
2843 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2791 2823 3603 3635 6534 6566 2791
Size: 32 780 32 2899 32 2799
Fragments arranged by size:
2899 2799 780 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2282 3325 1316
Size: 966 1043 4565
Fragments arranged by size:
4565 1043 966
XbaI T'CTAG_A
Cuts at: 3910 3910
Size: 6574
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3151 3151
Size: 6574
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6574
XmaI C'CCGG_G
Cuts at: 1870 3106 3899 1870
Size: 1236 793 4545
Fragments arranged by size:
4545 1236 793
XmnI GAAnn'nnTTC
Cuts at: 2625 3701 4703 2625
Size: 1076 1002 4496
Fragments arranged by size:
4496 1076 1002
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2164 4382 276
Size: 53 83 186 936 630 2218 2468
Fragments arranged by size:
2468 2218 936 630 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI
BstZ17I Bsu36I EcoRV FalI FseI NruI NsiI PacI
PfoI PspXI PsrI RsrII SacII SexAI SfiI SrfI
SwaI